Die muskelspezifischen RING-Finger Ubiquitin E3 Ligasen MuRF1, MuRF2 und MuRF3 werden mit verschiedenen zellulären Prozessen in Verbindung gebracht. MuRF1 und MuRF3 beteiligen sich am Abbau mehrerer Muskelstrukturproteine über das Ubiquitin Proteasom System (UPS) und spielen somit eine wichtige Rolle bei der Aufrechterhaltung der Skelett- und Herzmuskelstruktur und -funktion. MuRF1 wurde als Atrophie-Marker identifiziert, da seine Expression während der Muskelatrophie ansteigt, und MuRF2 und MuRF3 wirken bei der Stabilisierung von Mikrotubuli und Differenzierung von Myozyten mit. Dennoch sind bisher viele Aspekte der Funktion von MuRF-Proteinen ungeklärt. Die Domänenstruktur der MuRF-Proteine zeigt mehrere hochkonservierte Domänen, die sich an Protein-Protein Interaktionen beteiligen. Die Identifizierung und Charakterisierung ihres Interaktoms ermöglicht ein besseres Verständnis ihrer Funktionen. Aus diesem Grund wurden quantitative massenspektrometrische Analysen durchgeführt, um neue Interaktionspartner und Substrate für MuRF1, 2 und 3 zu identifizieren. Sorting nexin 5 (SNX5), eine Untereinheit des Retromers in Säugetieren, wurde als Interaktionspartner von MuRF3 identifiziert. SNX5, das eine wichtige Rolle in subzellulären Transport-Signalwegen spielt, interagierte über seine BAR-Domäne mit MuRF3. SNX5 und MuRF3 co-lokalisierten und assoziierten mit vesikulären Strukturen des subzellulären Transport-Signalweges. SNX5 wurde außerdem als Substrat von MuRF2 identifiziert. MuRF2 band und ubiquitinierte SNX5 in vivo und vermittelte damit dessen Abbau über das UPS. MuRF3 stabilisierte SNX5 durch die Inhibierung dieses Abbaus. Somit konnten MuRF2 und MuRF3 mit einem in subzellulärem Transport aktiven Protein in Verbindung gebracht werden, das direkt mit Mikrotubuli assoziiert und funktionell von einem stabilen Mikrotubuli-Netzwerk abhängig ist. Dies legt eine mögliche regulatorische Rolle von MuRF2 und MuRF3 in Mikrotubuli-abhängigen subzellulären Transportwegen nahe. / Muscle specific RING-Finger ubiquitin E3 ligases MuRF1, MuRF2 and MuRF3 have been implicated in several cellular functions. MuRF1 and MuRF3 have been shown to bind and degrade muscle contractile and structural proteins via the ubiquitin proteasome system (UPS), thus playing an important role in the maintenance of skeletal and cardiac muscle structure and function. MuRF1 is considered an atrophy marker since its expression increases during muscle atrophy. MuRF2 and MuRF3 are involved in myocyte differentiation and both bind to and stabilize microtubules. Nevertheless, many aspects of the functions of the MuRF-family are unknown. The domain structure of the MuRF family implicates several highly conserved domains involved in protein-protein interaction. Accordingly, one way to better understand the role of MuRF proteins in myocyte function and protein homeostasis is to identify and characterize their interactome. Therefore, quantitative mass spectrometric analysis was used to identify novel interaction partners and target proteins of MuRF1, 2 and 3. Sorting nexin 5 (SNX5), a mammalian retromer subunit which plays an important role in subcellular trafficking pathways, was identified as a novel interaction partner of MuRF3, with which it interacted via its Bin/Amphiphysin/Rvs (BAR)-domain. SNX5 and MuRF3 co-localized and associated with early endosomes, connecting the microtubule-binding MuRF3 to structures of subcellular trafficking pathway. SNX5 was also identified as a substrate of MuRF2, which interacted with and ubiquitinated SNX5 in vivo, mediating its degradation in a UPS-dependent manner. This MuRF2-mediated degradation was inhibited by MuRF3, which stabilized SNX5. Thus, MuRF2 and MuRF3 were linked to a subcellular trafficking protein, SNX5, which is directly associated with microtubules and functionally dependent on a stable microtubule network, suggesting a possible regulatory role of MuRF2 and MuRF3 in microtubule-dependent subcellular trafficking pathways.

Fehlgefaltete Proteine des sekretorischen Weges werden aus dem Endoplasmatischen Retikulum (ER) in das Zytosol transportiert und dort durch das Ubiquitin-Proteasom-System abgebaut. Dieser Qualitätskontrollmechanismus wird als Endoplasmatisches Retikulum-assoziierte Proteindegradation bezeichnet (ERAD). In der Bäckerhefe Saccharomyces cerevisiae stellt die HRD-Ubiquitinligase eine zentrale Komponente dieses Abbausystems dar. Eine Untereinheit dieses Multienzymkomplexes ist das ER-ständige Membranprotein Der1, das über den Faktor Usa1 an die Ubiquitinligase Hrd1 rekrutiert wird und ausschließlich für den Abbau löslicher luminaler ERAD-Substrate notwendig ist. Im Rahmen dieser Arbeit konnte gezeigt werden, dass der C-Terminus von Der1 die Interaktion zu Usa1 und damit die Rekrutierung des Proteins zur HRD-Ligase vermittelt. Usa1 wirkt nicht nur als Rekrutierungsfaktor, sondern induziert auch die Der1-Oligomerisierung. Punktmutationen in den Transmembrandomänen von Der1 beeinträchtigen die Dislokation luminaler Substratproteine aus dem ER. Um weitere Hinweise für eine Beteiligung von Der1 beim Substrattransport zu erhalten, wurde die Methode des zielgerichtetem in vivo photocrosslinking für Der1 angewendet. Hierbei wurden bestimmte Positionen von Der1 mit dem photoreaktiven Aminosäureanalogon p-Benzoylphenylalanin markiert, was die Ausbildung von Quervernetzungen von Der1 zu Interaktionspartnern nach einer UV-Bestrahlung ermöglichte. Schließlich konnte auf diese Weise eine räumliche Nähe der luminal exponierten Bereiche von Der1 zum Substratrezeptor Hrd3 gezeigt werden, während die Transmembransegmente Quervernetzungen zu Hrd1 ausbildeten. Beide Bereiche von Der1 konnten zudem mit einem luminalen ERAD-Substrat quervernetzt werden. Anhand dieser Ergebnisse wurde somit erstmals eine direkte Beteiligung von Der1 insbesondere in den ersten Schritten der Substratdislokation gezeigt, was eine Funktion von Der1 als zentrale Komponente des Exportkomplexes nahelegt. / Newly synthesized proteins of the secretory pathway are subjected to an efficient quality control system in the endoplasmic reticulum. In order to prevent a harmful aggregation misfolded proteins are exported via a largely unknown mechanism into the cytosol and degraded by the ubiquitin-proteasome system in a process termed ER associated degradation (ERAD). In the yeast Saccharomyces cerevisiae the HRD-ligase constitutes a central component of ERAD. A subunit of this multi-enzyme complex is the small multispanning membrane protein Der1, which is exclusively required for the degradation of misfolded ER luminal proteins but dispensable for the turnover of membrane-bound substrates. In this study a short conserved motif in the cytosolic carboxyterminus of Der1 was identified that mediates the binding to the HRD-ligase. Moreover, co-immunoprecipitation experiments show that Der1 forms oligomers, which relies on its assembly into the degradation complex. Mutations in the transmembrane domains of Der1 block the export of soluble proteins across the ER-membrane. To further investigate the function of Der1 in substrate dislocation an in vivo site-specific photocrosslinking approach was applied. Various positions of Der1 were labelled with the photoreactive amino acid analogue p-benzoyl-phenylalanine followed by UV irradiation of living cells expressing these Der1 constructs. The crosslinking experiments reveal a spatial proximity of ER luminal exposed parts of Der1 to the substrate receptor Hrd3. By contrast, the membrane-embedded domains of Der1 reside adjacent to the ubiquitin ligase Hrd1. Intriguingly, both regions also form crosslinks to a client protein. In summary the data of this work imply that multimeric Der1 initiates the export of aberrant polypeptides from the ER-lumen by threading such molecules into the ER-membrane and routing them to Hrd1 for ubiquitylation.

L'accumulation de gaz à effet de serre dans l'atmosphère, produite par des activités anthropiques notamment dans le secteur de l’énergie est une des causes principales du changement climatique. Par conséquent, réaliser une véritable transition énergétique par une décarbonisation des réseaux électriques est devenue un besoin urgent pour atténuer les effets du réchauffement climatique. Dans cette transition, l’introduction des énergies renouvelables a été initiée depuis plusieurs années, principalement en raison de la participation de nouveaux acteurs à ce marché. Aujourd’hui, l’un des grands défis est de maintenir l’équilibre et la sécurité du réseau électrique en tenant compte de la diversité et de la variabilité des ressources énergétiques renouvelables connectées au réseau. L’une des approches permettant de régler ce problème et d’accroître la flexibilité du réseau électrique par ce que l’on désigne comme la Réponse à la Demande (RD). Cette thèse examine précisément ces nouvelles approches et montre l’intérêt de repenser les relations entre les différentes partie-prenantes pour faire émerger des nouveaux modèles d’affaires afin de déployer de nouvelles innovations au service de la transition énergétique. La méthodologie de recherche mise en œuvre de cette thèse consiste en une revue systématique de la littérature et une étude des données empiriques de 15 jeunes entreprises européennes du secteur de l’énergie. En conséquence, la thèse fournit à la communauté de la recherche (1) une méthode de classification pour catégoriser les différents modèles d’affaires de l’énergie (MAEs) et présente une première synthèse des MAE identifiés dans la littérature; (2) un cadre d’analyse des start-ups dans le secteur de l’énergie, complété par l’analyse de 15 start-ups de ce domaine; (3) un outil conceptuel pour l'innovation en matière de RD, appelé Canevas de Modèle d'Affaires de Réponse de Demande (CMARD), qui comprend 12 éléments interreliés. Ce canevas vise à évaluer les activités des offres de RD et à soutenir l'émergence de nouveau modèles d'affaires de RD. Ces résultats permettent de proposer un cadre analytique simplifié des pratiques existantes et peuvent également aider des entrepreneurs ou décideurs à explorer et concevoir de nouvelles offres sur le marché de la réponse à la demande. / The accumulation of greenhouse gases in the atmosphere, produced by human activities in the energy sector is one of the main causes of climate change. Therefore, the decarbonization of power systems has become an urgent need the mitigate to the effects of climate change and achieve the energy transition. The share of renewable energy technologies has been increasing mainly due to the participation of new market players. Today, however, one of the great challenges is to maintain the electricity system’s balance and security despite the large amount of renewable energy resources connected to the grid. One of the approaches to deal with this issue and to increase power system flexibility is the Demand Response (DR). Moreover, scholars argue that business model innovation can act as an energy transition driver. This thesis examines business model innovations in the context of the energy transition by investigating emerging start-up business models. The implemented research methodology in this thesis consists of a systematic literature review and an investigation of empirical data of 15 European energy start-ups. As a result, the thesis provides the research community with (1) a grouping method to classify different Energy Business Models (EBMs) and an initial synthesis of the EBMs identified in the literature; (2) a framework to analyse start-ups in the energy sector, completed with the analysis of 15 energy start-ups; (3) and a conceptual tool for DR innovation, named as the Demand Response Business Model Canvas (DRBMC), which includes 12 interrelated elements. This canvas aims at evaluating DR activities and supporting the emergence of new DR business models. These results can also help entrepreneurs explore new demand response market opportunities, enabling a better understanding and providing a simplified analytic framework of existing business practices.

Ce travail porte sur l’étude des interfaces semi-conducteur/diélectrique et semiconducteur/électrode dans les transistors organiques à effet de champ (OFETs). En effet, le transport et l’injection des charges se trouvent affectés par la qualité de ces interfaces.L’objectif est donc la compréhension de l’influence des caractéristiques morphologiques(rugosité, énergie de surface) et électroniques (travail de sortie) du diélectrique ou del’électrode sur les performances des OFETs.Dans un premier temps, des OFETs sur substrats de silicium à base de pentacène ontété fabriqués et les interfaces traitées à l’aide de monocouches auto-assemblées (SAMs). Legreffage des SAMs tels que l’OTS8 ou l’OTS, en neutralisant les groupes hydroxyles et enprésentant une surface apolaire, conduit à une réduction de la densité de pièges en surface. Deplus, les pièges présents dans la couche active et dus aux joints de grains sont moinsnombreux grâce à une croissance 2D en larges grains du pentacène sur OTS. Cesmodifications de l’interface sont mises en évidence par une réduction de la tension de seuil,de la pente sous le seuil ainsi que de l’hystérésis. Le transport ainsi favorisé des chargespermet une amélioration de la mobilité jusqu’à 0,6 cm2/Vs.Nous nous sommes également intéressés à l’interface semi-conducteur/électrode et àsa modification par des SAMs fluorés tels que le PFBT, le PFHT et le PFDT. L’influence desSAMs est présente tant au niveau morphologique, en améliorant la continuité de croissance dupentacène à la jonction diélectrique/électrode qu’au niveau électronique en augmentant letravail de sortie de l’électrode. La réduction de la résistance de contact RC souligne clairementces modifications et conduit à des mobilités maximales de 0,6 cm2/Vs. Par la suite, nousavons choisi de modifier ces deux interfaces dans un même dispositif, ce qui nous a permisd’atteindre des mobilités moyennes élevées de 1,3 cm2/Vs.La dernière partie de ces travaux a été dédiée à la fabrication d’OFETs basse tension àbase de pentacène ou de C60 sur substrats de verre. Le caractère basse tension de cesdispositifs est rempli grâce à l’utilisation d’un diélectrique composé de deux couches : undiélectrique à forte constante diélectrique, l’oxyde d’aluminium, et une fine couche d’undiélectrique à faible constante diélectrique comme les SAMs (C8-PA ou C18-PA) ou lespolymères (PMMA ou PVT). Cette combinaison permet d’atteindre des mobilités(m = 0,4 cm2/Vs) encourageantes pour des OFETs de type n ainsi que de faibles hystérésis(<0,1 V) dans le cas d’OFETs de type p. / The charge transport and injection are strongly dependant of the semiconductor/dielectric and semi-conductor/electrode interfaces quality. Therefore, this studyfocuses on these interfaces in organic field effect transistors (OFETs). The goal is theunderstanding of the relation between the dielectric (roughness, surface energy) or electrode(work function) characteristics and the OFETs performances.First, we investigate the influence of the interfaces modification by SAMs (SelfAssembled Monolayers) in pentacene based OFETs on silicon substrates. Due to the SAMsgrafting such as OTS8 or OTS, the hydroxyls groups are neutralized and the dielectric showsan apolar surface leading to the reduction of the charge traps density. Moreover, a 2Dpentacene growth with large grains on OTS surface contributes to the decrease of the chargetraps density in the bulk. The threshold voltage, subthreshold swing and hysteresis decreasesgive rise to these modifications. The improvement of the charge transport allows us to reachmobility up to 0.6 cm2/Vs.Then, we investigate the electrode surface treatment by fluorinated SAMs such asPFBT, PFHT or PFDT. The better pentacene layer continuity and the increased electrodework function emphasize the morphologic and electronic influences of the SAMs. Thesemodifications lead to the contact resistance reducing and in consequence to an enhancedmobility up to 0.6 cm2/Vs. Finally, devices with a combination of the interfaces treatmentpresent high mean mobility of 1.3 cm2/Vs.On the final part of this study, we concentrate on low voltage C60 or pentacene basedOFETs on glass substrates. Using a dielectric composed of a high-k dielectric as AlOx and athin layer of a low-k dielectric such as phosphonic SAMs (C8-PA or C18-PA) or polymers(PMMA or PVT) allow us to achieve this low voltage condition. The mobility obtained withn-type OFETs (m = 0.4 cm2/Vs) and the small hysteresis (<0.1 V) in p-type OFETs arepromising.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / This Master s Dissertation is proposes the development of a charge management system of a bank of 16 lead-acid batteries with 7Ah load capacity, commonly used in Uninterruptible Power Supplies (UPS). This system aims to ensure that these batteries reach a full charge status faster than the conventional way. To this, it was chosen to make the modification of the pulsed current charging method, in order to reduce problems, such as elevation of bus voltage to return the energy to it, or dissipation of that energy in a resistance, resulting in lower system efficiency, inherent in the method taken as a basis when the battery power withdrawal interval occurs. For the development of the suggested charging system the static converter Full-Bridge Isolated with modulation Phase Shift was chosen. To implement the digital control as well as its practical development in the same laboratory the simplified modeling of this converter was made. The results of simulation and experimental to validate the method are presented. / Esta dissertação de mestrado tem como proposta o desenvolvimento de um sistema de gerenciamento de carga de um banco de 16 baterias de chumbo ácido, com capacidade de carga de 7Ah, comumente utilizado em Fontes Ininterruptas de Energia (UPS). Este sistema visa garantir que estas baterias cheguem a um estado de carga completo e de forma mais rápida que a convencional. Para isto, foi escolhido fazer a modificação do método de carga de corrente pulsada, com o objetivo de reduzir problemas inerentes do método tomado como base, quando ocorre o intervalo de retirada de energia da bateria, tais como: elevação da tensão do barramento ao retornar a energia para ele, ou, dissipar esta energia em uma resistência, o que acarreta em baixa eficiência do sistema. Para o desenvolvimento do sistema de carga sugerido foi escolhido o conversor estático Full-Bridge Isolado com modulação Phase Shift. Foi feita a modelagem simplificada deste conversor, para a implementação do controle digital, assim como o desenvolvimento prático do mesmo em laboratório. São apresentados os resultados de simulação e experimentais para validar o método.

Chez Arabidopsis thaliana, les protéines SIAMESE-RELATED (SIM/SMR1 à 13) forment une famille plante-spécifique d’Inhibiteurs de Kinase Cycline-dépendante (CKI), homologue des Kip-Related Proteins. SIM et SMR1 sont des régulateurs positifs de la transition du cycle mitotique vers l’endoréplication. L’expression des gènes SIM/SMR est induite en réponse àdes stress. L’un des stress abiotiques majeurs pour les plantes est la sécheresse. Les SIM/SMR pourraient être dégradées par la voie de la protéolyse spécifique de l’Ubiquitin Proteasome System (UPS). Les SIM/SMR sont de bons candidats pour relier l'activité du cycle cellulaire aux stimuli de l'environnement. Ce travail a démontré l’implication de la protéolyse UPS dans le contrôle posttraductionnel de tous membres SIM/SMR testés. Il démontre que SIM, SMR2 et SMR1 sont nécessaires à l’endoréplication des cellules foliaires. Lors d’un stress hydrique, l’expression des gènes SIM, SMR1, SMR3 et SMR5 est induite. Le profil spatio-temporel de ces inductions a mis en évidence deux groupes de gènes avec des fonctions distinctes. Les mutants sim, smr5 et sim.smr1.smr2 sont hypersensibles au stress hydrique. / In Arabidopsis thaliana, the SIAMESE-RELATED proteins (SIM/SMR1 to 13) are a plantspecific family of Cyclin-dependent Kinase Inhibitors (CKIs), homologous to the Kip-Related Proteins. SIM and SMR1 are positive regulators of the switch from mitotic cycle to endoreplication. The expression of SIM/SMRs genes is induced in response to stress. One of the major abiotic stress for plants is the drought stress. The SIM/SMRs could be degraded through the specific proteolysis of Ubiquitin Proteasome System (UPS). The SIM/SMRs proteins are good candidates to link cell cycle activity with environmental stimuli.This research work has shown the involvement of the UPS proteolysis in the posttranslational control of all the tested members of the SIM/SMR family. It also shows that SIM, SMR2 and mostly SMR1 are required in endoreplication of leaf cells. During drought stress, the expression of SIM, SMR1, SMR3 and SMR5 genes is induced. The spaciotemporal pattern of those inductions revealed two groups of genes with distinct functions. In addition, the sim, smr5 and sim.smr1.smr2 loss-of-function mutants tested are hypersensitive to drought stress.

Submitted by isabela.moljf@hotmail.com (isabela.moljf@hotmail.com) on 2017-03-02T11:39:15Z No. of bitstreams: 1 fredericotoledoghetti.pdf: 2816885 bytes, checksum: 98c4c49d919a94d51a94ff86a91c33a1 (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2017-03-06T19:32:09Z (GMT) No. of bitstreams: 1 fredericotoledoghetti.pdf: 2816885 bytes, checksum: 98c4c49d919a94d51a94ff86a91c33a1 (MD5) / Made available in DSpace on 2017-03-06T19:32:09Z (GMT). No. of bitstreams: 1 fredericotoledoghetti.pdf: 2816885 bytes, checksum: 98c4c49d919a94d51a94ff86a91c33a1 (MD5) Previous issue date: 2009-05-26 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / É apresentado, neste trabalho, um estudo sobre topologias alternativas de fontes ininterruptas de energia do tipo Delta, para o emprego em cargas monofásicas de até 1kVA. O estudo prevê a inserção de um conversor CC-CC entre o conversor shunt e o banco de baterias. Tal modificação em relação à UPS do tipo Delta convencional otimiza o projeto do banco de baterias e requisita um menor número de células em série para sua confecção. Além disso, a proposta elimina o uso de transformadores de baixa freqüência, considerados em alguns trabalhos anteriores, o que também contribui para a redução do peso e volume do sistema. Duas diferentes topologias para o conversor CC-CC foram investigadas e comparações de desempenho e viabilidade foram realizadas. A topologia que apresentou melhor desempenho nos quesitos custo, volume e peso, foi selecionada para testes em bancada. Ao final, resultados simulados e práticos são apresentados para validação do modelo. / This dissertation presents a study on single-phase Delta-UPS topological alternatives for low power and low cost applications. The proposed topology has a DC-DC converter connected between the shunt inverter and the battery bank/series converter DC terminals, respectively. This modification will allow the downsizing of the DC battery bank as well as the removing of the series converter transformer. Two different topologies of the DC DC converter were investigated and a comparative study among them will be accomplished. Simulation results are presented and experimental results will be included to validate the proposed study.

Name Relation between physical fitness and study results of primary school students Goal The goal of the thesis is to examine physical fitness of primary school students, compare it with their study results, and see if there is any relatioship between these two variables. Methods Physical fitness is tested with tests and norms of Unifittest (6-60) (Měkota, Kovář, 2002) and study results are generated with questionnaires. The answers are then matched with Unifittest's results of each student. Results are processed with the help of statistical methods. Results The results of Unifittest (6- 60) showed above-average or well above-average level of fitness with 89 % of individuals. Only 11 % of participants reached the average score. The best performance was detected with girls of the 9 ͭ ͪ grade and boys of the 7 ͭ ͪ grade. Spearman correlation coefficient showed a medium-strong relation between the Unifittest (6- 60) results and grades from Czech language, foreign language, and mathematics in 2 categories. 6 ͭ ͪ grade girls had the correlation rₛ= 0,51 and 8 ͭ ͪ grade girls rₛ= 0,56. Other categories showed very weak correlation relation. Keywords Unifittest, physical fitness, studying, youth, sit-ups, pull-ups, long jump, Cooper test

Managing the new product development (NPD) process, especially the first phase, the front end of innovation (FEI), is crucial for companies today in order to develop successful products and reach process and product sustainability. This has proved to be a significant challenge for start-ups since 90 percent of them fail (Krishna et al., 2016). This explorative study investigates how prototyping can be used in FEI, with a focus on Swedish start-up companies. A great deal of research has been published about prototyping in the later stages in the development process, and about FEI in general. Regarding prototyping in FEI, the existing literature is mainly located in the product design area. A substantial research gap has also been identified in a start-up context regarding prototyping in FEI. To fill in this gap, a qualitative approach was used collecting data form three different perspectives; start-up accelerators, start-ups employees and users. To accumulate these three perspectives, two sets of semi-structured interviews and two focus groups were conducted. Thematic analysis was used to analyze the collected data. The empirical findings align with the existing literature in several areas, but also gain new insights, contributing to filling the research gap. To conclude, there are a number of different specific prototyping purposes and methods, as well as crucial recommendations, that start-ups should consider in FEI to succeed. The thesis highlights that start-ups should use simple prototypes early in FEI, iterating the prototyping efforts, and gradually increasing the accuracy of the prototype during the FEI.

Since the majority of emerging start-ups are not able to survive on the market, this study investigates business models in four start-up companies from two different industries in order to better understand how their business models relates to their success. This study is commissioned by a start-up who are about to begin their business in Stockholm. The purpose of this study is to make an assessment of the components in the utilized business models of growing start-ups in markets with different level of innovation. The investigation is made based on how trends and competition in the industry affect the business model and what the similarities are between the business model components of successful start-ups in two different industries. This study contribute to literature on start-ups’ business models by beginning to map differences and similarities in start-up’ business models as well as how these are affected by and adapted to the industry. It also helps start-ups understand the importance and usage of different business model components. In order to make an assessment of different business models, a multiple case study approach is adopted where each case is a start-up company. The analysis then follows a 2x2 framework and is sorted by Business Model Canvas. Three analyses are made, one within each industry and one between the two industries. The first analysis compares the business models of two companies in the industry of electricity consumption measurements and finds that trends regarding the environment and innovation affects both the industry and its’ competitors. The second analysis compares the business models of two companies in the package deliveries industry and trends regarding online shopping are found to affect this industry. The third analysis compares the two industries and both differences and similarities are found. The main findings regarding the business model components are then applied to the commissioner company and other emerging start-ups. Findings shows that the investigated start-ups have adapted the business model components to trends in the respective industry. The business model also adapts to the technological development in the associated industry and in other industries as well. The use of social channels seems to affect the customer relationships. Competition have affected the business models’ of the investigated start-ups and it is noticed that international and national goals, such as lowering  the environmental impact, also affect the industry and the business models since these goals can encourage and push for change. Furthermore, each of the start-ups’ business model components have similarities even though the B2B, B2C and B2B2C strategies create differences. Furthermore, all of the start-ups are working with the trial and error approach, sustainability and environmental impact when developing their business model. / Eftersom majoriteten av alla start-up företag inte lyckas överleva på marknaden undersöker denna studie affärsmodeller i fyra start-ups i två olika industrier för att bättre förstå hur deras affärsmodeller har påverkat deras framgång. Studien utförs i Stockholm där uppdragsgivaren verkar. Syftet med studien är att göra en utvärdering av komponenterna i den verksamma affärsmodellen i växande start-up företag som verkar på marknader med olika innovationsnivåer. Studien utförs baserat på hur affärsmodellen anpassas efter trender och konkurrens i branschen och vilka likheter som finns i dessa affärsmodellers komponenter. Denna studie kan bidra till nuvarande litteratur om start-ups affärsmodeller genom att börja kartlägga affärsmodellernas likheter och skillnader och se hur de är anpassade efter branschen. Studien kan även hjälpa start-ups förstå nyttan och användningen av de olika komponenterna i deras affärsmodell. För att utföra jämförelsen användes en flerfallsstudie där de olika fallen är olika start-up företag. Analysen utfördes med hjälp av en 2x2 modell och är strukturerad efter Business Model Canvas. Totalt är tre analyser är genomförda, en inom varje bransch och en mellan de två branscherna. Den första analysen jämför affärsmodellerna för de två start-up företagen i branschen för elkonsumtionsmätningar och finner att innovation och trender från miljöaspekter påverkar dess företag. Den andra analysen jämför affärsmodellerna för de två start-up företagen i branschen för paketleveranser och finner att dessa företag påverkas av trenden webbshopping. Tredje och sista analysen jämför likheter mellan branscherna och finner både skillnader och likheter. Dessa kopplas sedan till uppdragsgivaren och andra nya start-ups i diskussionskapitlet. Analysen visar på att de undersökta start-up företagen har anpassat sina affärsmodellers komponenter efter trender i branschen. Affärsmodellen är också anpassad efter den tekniska utvecklingen i både den egna och andras branscher. Sociala kanaler verkar påverka kundrelationerna. Vidare har konkurrensen på marknaden påverkat affärsmodellerna, liksom nationella och internationella mål inom exempelvis miljöpåverkan som uppmuntrar förändringar. Slutligen har varje enskild komponent i affärsmodellerna likheter, även om de olika strategierna B2B, B2C och B2B2C skapar skillnader. Fortsättningsvis har alla undersökta start-ups testat sig fram och jobbar med hållbarhet och miljöpåverkan för att utveckla sina affärsmodeller.