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Molecular Pathogenesis of Cervical Carcinoma : Analysis of Clonality, HPV16 Sequence Variations and Loss of HeterozygosityHu, Xinrong January 2001 (has links)
<p>A previous model of morphological pathogenesis assumed that cervical carcinoma is of monoclonal origin and progresses through multiple steps from normal epithelium via CINS into invasive carcinomas. The aim of this study was to investigate the molecular mechanism of pathogenesis of cervical neoplasia. </p><p>In the clonality study, we found that 75% (6/8) of informative cases of cervical carcinoma had identical patterns of loss of heterozygosity (LOH) in the multiple synchronous lesions, while the remaining cases had different LOU patterns. In an extensively studied "golden case", the multiple carcinoma and cervical intraepithelial neoplasia (CIN) lesions could be divided into several different clonal groups by the X-chromosome inactivation patterns, HPV 16 mutations and LOH patterns. The biggest clonal family included one CIN II, one CIN III and four carcinoma samples, while four other monoclonal families of carcinoma did not include CIN lesions. These results suggested that cervical carcinoma can be either monoclonal or polygonal and contains clones developing either directly or via multiple steps. In the study of HPV types and HPV16 variations, the results confirmed that specific HPV types are the cause of cervical carcinoma but failed to support the previous opinion that HPV16 E6 variants are more malignant than the prototype. We established a novel classification called oncogene lineage of HPV16, and found that additional variations of HPV 16 oncogenes might be a weak further risk factor for cervical carcinoma. In the study of LOH, we found that interstitial deletion of two common regions of chromosome 3p, i.e., 3p2l.1-3p2l.3, and 3p22, was an early event in the development of cervical carcinoma. The results showed that the hMLH1 gene, located in 3p22 and showing LOH in 43% of the studied cases, was not involved in the development of cervical carcinoma because neither the expression level of protein nor the gene sequence was altered in these cases. </p><p>In summary, a suggested model of molecular pathogenesis of cervical carcinoma is as follows. Specific types of HPV infect one or more committed stem cells in the basal layer of the epithelium. Fully efficient LOH events turn one (monoclonal origin) or more (polyclonal origin) HPV-infected stem cells into carcinoma cells without CIN steps. Less efficient LOH events would lead to CIN steps where some other unknown factors require to be added to facilitate the formation of carcinoma. In the absence of LOH events no carcinoma develops from the HPV-infected stem cells.</p>
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Molecular Pathogenesis of Cervical Carcinoma : Analysis of Clonality, HPV16 Sequence Variations and Loss of HeterozygosityHu, Xinrong January 2001 (has links)
A previous model of morphological pathogenesis assumed that cervical carcinoma is of monoclonal origin and progresses through multiple steps from normal epithelium via CINS into invasive carcinomas. The aim of this study was to investigate the molecular mechanism of pathogenesis of cervical neoplasia. In the clonality study, we found that 75% (6/8) of informative cases of cervical carcinoma had identical patterns of loss of heterozygosity (LOH) in the multiple synchronous lesions, while the remaining cases had different LOU patterns. In an extensively studied "golden case", the multiple carcinoma and cervical intraepithelial neoplasia (CIN) lesions could be divided into several different clonal groups by the X-chromosome inactivation patterns, HPV 16 mutations and LOH patterns. The biggest clonal family included one CIN II, one CIN III and four carcinoma samples, while four other monoclonal families of carcinoma did not include CIN lesions. These results suggested that cervical carcinoma can be either monoclonal or polygonal and contains clones developing either directly or via multiple steps. In the study of HPV types and HPV16 variations, the results confirmed that specific HPV types are the cause of cervical carcinoma but failed to support the previous opinion that HPV16 E6 variants are more malignant than the prototype. We established a novel classification called oncogene lineage of HPV16, and found that additional variations of HPV 16 oncogenes might be a weak further risk factor for cervical carcinoma. In the study of LOH, we found that interstitial deletion of two common regions of chromosome 3p, i.e., 3p2l.1-3p2l.3, and 3p22, was an early event in the development of cervical carcinoma. The results showed that the hMLH1 gene, located in 3p22 and showing LOH in 43% of the studied cases, was not involved in the development of cervical carcinoma because neither the expression level of protein nor the gene sequence was altered in these cases. In summary, a suggested model of molecular pathogenesis of cervical carcinoma is as follows. Specific types of HPV infect one or more committed stem cells in the basal layer of the epithelium. Fully efficient LOH events turn one (monoclonal origin) or more (polyclonal origin) HPV-infected stem cells into carcinoma cells without CIN steps. Less efficient LOH events would lead to CIN steps where some other unknown factors require to be added to facilitate the formation of carcinoma. In the absence of LOH events no carcinoma develops from the HPV-infected stem cells.
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Mental retardation in children : an epidemiological and etiological study of mentally retarded children born 1959-1970 in a northern Swedish countyK:son Blomquist, Hans January 1982 (has links)
In an unselected series of mentally retarded children in the county of Västerbotten, Sweden, the annual incidence of children with severe mental retardation (SMR) (IQ < 50) and alive at the age of one year decreased from 5.3 per 1,000 in 1959 - 1963 to 3.1 per 1,000 in 1967 -1970. This was mainly due to a decrease in the incidence of Down's syndrome. In parallel the proportion of mothers 35 years of age or more at the birth of the child decreased significantly. The prevalence of children with SMR in 1976 was 3.5 per 1,000. The main cause of the SMR was prenatal in 70 percent, perinatal in 8 percent and postnatal in 1 percent. The cause of the SMR was untraceable in 20 percent of the cases. Associated CNS-handicaps occurred in 52 percent of the cases. The annual incidence of mildly mentally retarded children (IQ 50 - 69) registered at the Bureau for Provision and Services for Mentally Retarded was 4.2 per 1,000 and the prevalence in 1979 was 3.8 per 1,000. The cause of the mild mental retardation (MMR) was untraceable in 43 percent. Prenatal causes were identified in as many as 43 percent. Perinatal causes were found in 7 percent and postnatal causes in 5 percent of the cases. Associated CNS-handicaps occurred in 30 percent of the cases.A syndrome of mental retardation with X-linked inheritance not recognized previously in Sweden was characterized clinically (mainly in boys, machro-orchidism, verbal disabilities) and cytogenetically (a fragile site on the X-chromosomes seen after cui turing in special folic acid deficient media) and found to have a prevalence of 6 percent in the population of severely mentally retarded boys. This makes this syndrome the next most common cause of SMR in boys after Down's syndrome. The chromosomal fragility was also identified in female carriers, which has implications for genetic counselling.Through identification of an untreated Phenylketonurie mother giving birth to five severely mentally retarded children, attention was focused on the risks for the fetus of the growing number of Phenylketonurie women identified neonatally and treated di etarily but untreated after the age of 10 - 15 years.Great improvement in intellectual and social ability was seen in a boy with phenylketonuria although the dietary treatment was not introduced until the age of eight years.Heavy irradiation of a fetus late in gestation caused mental retardation, microcephaly, stunted growth, and eye and teeth abnormalities, although such abnormalities are thought not to result from irradiation after 20 weeks of pregnancy. / <p>Endast s.1-71: sammanfattningen (kappan) i fulltexten.</p><p>Ej med i fulltexten s.72-145: 7 delar.</p> / digitalisering@umu
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Cell autonomous and cell non-autonomous effects of mosaic Mecp2 expression on layer V pyramidal cell morphology in a mouse model of Rett SyndromeRietveld, Leslie A. 19 December 2012 (has links)
Rett Syndrome (RTT) is a neurodevelopmental disorder primarily caused by mutations in the X-linked gene methyl-CpG-binding protein 2 (MECP2). The mosaic brain environment in heterozygous (MECP2+/-) females consists of both MeCP2-wildtype (MeCP2+) and Mecp2-mutant (MeCP2-) neurons. To separate possible cell autonomous and cell non-autonomous effects three-dimensional morphological analysis was performed on individually genotyped layer V pyramidal neurons in the primary motor cortex of heterozygous (Mecp2+/-) and wild-type (Mecp2+/+) mature female mice (>8 months old) from the Mecp2tm1.1Jae line. Mecp2+/+ neurons and Mecp2+ were found to be indistinguishable while Mecp2- neurons have significantly reduced basal dendritic length (p<0.05), predominantly in the region 70-130 μm from the cell body, culminating in a total reduction of 15%. Mecp2- neurons have three (17%) fewer total branch points, lost specifically at the second and third branch orders. Thus the reduced total dendritic length in Mecp2- neurons is a result of fewer higher-order branches. Soma and nuclear areas of 30 Mecp2+/- female mice (5-21 months) with X chromosome inactivation (XCI) ratios ranging from 12% to 56% were analyzed. On average Mecp2- somata and nuclei were 15% and 13% smaller than Mecp2+ neurons respectively. The variation observed in the soma and nuclear sizes of Mecp2- neurons was not due to age, but was found to be correlated with the XCI ratio. Animals with a balanced XCI ratio (approximately 50% Mecp2-) were found to have Mecp2- neurons with a less severe cellular phenotype (11-17% smaller than Mecp2+). Animals with a highly skewed XCI ratio favouring expression of the wild-type allele (less than 30% Mecp2-) were found to have a more severe Mecp2- cellular phenotype (17-22% smaller than Mecp2+). These data support indicate that mutations in Mecp2 exert both cell autonomous and cell non- autonomous effects on neuronal morphology. / Graduate
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Mosaicismo e evolução do perfil epigenético durante a gravidez / Mosaicism and evolution of epigenetic profile during pregnancyKarina Bezerra Salomão 06 March 2013 (has links)
O imprinting genômico, processo regulado epigeneticamente segundo o qual os genes se expressam de acordo com sua origem parental (paterna ou materna), está envolvido no desenvolvimento placentário. Na região cromossômica 11p15.5 encontram-se vários genes importantes para o desenvolvimento fetal e da placenta, os quais são regulados por duas principais regiões controladoras de imprinting (ICR1 e 2) onde se encontram as regiões diferencialmente metiladas H19DMR e KvDMR1, respectivamente. O imprinting genômico e a inativação aleatória do cromossomo X são processos epigenéticos presentes em mamíferos placentários. O presente trabalho teve como objetivo principal verificar a presença de mosaicismo do perfil epigenético entre tecidos extraembrionários de estágios precoces da gravidez (primeiro trimestre), e em vilosidade coriônica de placentas a termo (terceiro trimestre). Foram coletadas amostras de 10 gestações de primeiro trimestre (vilosidade coriônica, âmion, membrana de cordão umbilical e tecido embrionário) e 14 de terceiro trimestre (vilosidade coriônica), das quais 10 foram consideradas como controles e quatro utilizadas para estudo de mosaicismo restrito à vilosidade coriônica (coleta de amostras de todos os cotilédones). Após extração do DNA, foi utilizado o Método de Digestão Enzimática Sensível à Metilação Associada à PCR em Tempo Real para o estudo do padrão de metilação da KvDMR1 e da H19DMR em diferentes tecidos do primeiro trimestre gestacional e em tecido placentário do terceiro trimestre. O padrão de inativação do cromossomo X foi avaliado em todos os cotilédones de duas placentas a termo, de fetos do sexo feminino, por meio do ensaio do receptor de andrógeno humano (HUMARA assay), utilizando eletroforese capilar, e com acréscimo de um novo marcador de inativação do cromossomo X (ICX1). Na análise estatística foram utilizados o teste t não pareado, teste de Turkey e teste t pareado. A média de metilação da KvDMR1 das amostras de vilosidade coriônica do primeiro trimestre gestacional foi estatisticamente diferente da média de metilação do terceiro trimestre. Enquanto que a metilação da H19DMR não apresentou diferença estatística entre amostras de vilosidade coriônica do primeiro e do terceiro trimestre gestacionais. Com relação ao mosaicismo, a KvDMR1 não apresentou variação com relação ao tamanho ou a posição dos cotilédones, enquanto que a H19DMR apresentou diferença estatisticamente significativa na média de metilação com relação ao tamanho dos cotilédones e ao posicionamento nos quadrantes; em consequência da hipometilação em cotilédones pertencentes a uma das placentas estudadas. Não foram observadas diferenças estatisticamente significativas na média de metilação da KvDMR1 e da H19DMR entre diferentes tecidos das amostras do primeiro trimestre gestacional. No entanto, a comparação entre tecidos pareados de um mesmo indivíduo mostrou que a metilação não é correspondente entre os tecidos. Os dados obtidos mostram que o imprinting genômico provavelmente é um processo dinâmico, que evolui ao longo da gestação, estando relacionado a formação e ao amadurecimento da placenta. No presente estudo foi possível verificar que cotilédones de uma mesma placenta apresentam diferentes padrões de inativação do cromossomo X. Diferenças que podem ser explicadas pela expansão clonal das células trofoblásticas progenitoras com o cromossomo X paterno ou o cromossomo X materno inativo. Devido à variabilidade epigenética, exames em tecidos placentários devem considerar as diferenças intra-placentárias e as diferenças entre tecidos embrionários e extraembrionários. / Genomic imprinting, a mechanism of allele-specific expression depending on parental origin, is an epigenetic process that regulates the expression of many genes involved in placental development. Several important genes for fetal and placental growth are located on the human chromosome region 11p15.5, which are regulated by two imprinting control regions (ICR1 e 2), which have the differentially methylated regions H19DMR and KvDMR1, respectively. Genomic imprinting and random inactivation of X chromosome are two epigenetic processes present in placental mammals. The present study aimed to verify the presence of epigenetic mosaicism between extra-embryonic and embryonic tissues from early stages of pregnancy (first trimester), and in chorionic villi of term placentas (third trimester). Samples were collected from 10 pregnancies in the first trimester (chorionic villous, amnion, umbilical cord membrane, and embryonic tissue) and 14 from third trimester (chorionic villus sampling), of which 10 were considered as controls and four used to study mosaicism restricted to chorionic villi (sampling of all cotyledons). After DNA extraction, we used real time PCR associated to enzymatic restriction with a methylation sensitive enzyme to study the methylation pattern of KvDMR1 and H19DMR in different tissues from first trimester and placental third trimester tissue. The pattern of X chromosome inactivation was evaluated in all cotyledons from two term placentas of female fetuses, using the human androgen receptor (HUMARA) assay, capillary electrophoresis, and adding a new X chromosome inactivation (ICX1) marker. Unpaired and paired t and Turkey tests were used in statistical analysis. The average methylation of KvDMR1 of chorionic villi samples in first trimester was statistically different from average methylation of the third trimester. While the methylation of H19DMR showed no statistically significant difference between chorionic villi samples in the first and third trimester of pregnancy. In relation to the mosaicism, the KvDMR1 methylation did not vary in respect to the size or position of the cotyledons, while H19DMR showed statistically significant difference in average methylation relative to the size of the cotyledons, to the position in quadrants, due to the hypomethylation in cotyledons from one studied placenta. There were no statistically significant differences in the mean methylation KvDMR1 and H19DMR among different tissues from the first trimester of pregnancy, however, the comparison between paired tissues from the same individual showed that the methylation is different between tissues. The data from this study showed that genomic imprinting is probably a dynamic process and evolved across human pregnancy. This process is probable connected to placenta formation and maturation. We observed different patterns of X chromosome inactivation in cotyledons from the same placenta. This difference could be explained by clonal expansion of a limited number of trophoblastic progenitor cells with either an inactive maternal or paternal X chromosome. Due to the epigenetic variability, placental tissue examinations must consider the differences intra-placental and differences between embryonic and extra-embryonic tissues.
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Étude de la reprogrammation du chromosome X dans les cellules souches embryonnaires et extra-embryonnaires au cours du développement préimplantatoire murin / Study of X chromosome reprogrammation in embryonic and extra-embryonic stem cells during mouse preimplantation developmentPrudhomme, Julie 26 September 2014 (has links)
Chez les Mammifères femelles, l’extinction transcriptionnelle d’un des deux chromosomes X pendant l’embryogénèse précoce compense le déséquilibre de dose des gènes liés à l’X entre les sexes. L’inactivation aléatoire du chromosome X est mise en place dans la masse cellulaire interne du blastocyste et maintenue jusqu’à l’âge adulte dans le soma. Chez certains Euthériens incluant la souris, les tissus extra-embryonnaires (trophectoderme et endoderme primitif) montrent une inactivation soumise à empreinte du X paternel. Le statut inactif du Xp peut être étudié ex vivo dans les cellules souches trophoblastiques (TS) dérivées du trophectoderme. Nous avons pu sélectionner des cellules TS montrant une réactivation partielle du Xp ou bien une inversion complète du profil d’inactivation. Ceci révèle une plasticité épigénétique accrue de l’inactivation dans le trophectoderme par au soma.L’inactivation aléatoire du chromosome X est récapitulée pendant la différenciation des cellules souches embryonnaires (ES), qui servent de modèle cellulaire. Ce processus est déclenché par l’accumulation en cis du long ARN non codant Xist qui crée un domaine nucléaire répresseur autour du futur chromosome X inactif. Avant la différenciation, l’accumulation de Xist est réprimée par un autre long ARN non codant, Tsix, qui est transcrit en antisens de Xist. Afin d’adresser la dynamique fonctionnelle des ARN Xist et Tsix, nous avons inséré différents motifs d’étiquetage au locus Xist/Tsix endogène. Incorporés dans l’ARN sens ou antisens, ces étiquettes sont reconnues spécifiquement par des molécules fluorescentes, permettant ainsi la visualisation de ces transcrits dans les cellules vivantes. / In female Mammals, the transcriptional silencing of one of the two X chromosomes during early embryogenesis compensates the dosage disequilibrium of X-linked genes between sexes. Random X chromosome inactivation occurs in the inner cell mass of the blastocyst and is maintained through adult life in the soma. In some Eutherian species including mice, extraembryonic tissues (trophectoderm and primitive endoderm) exhibit imprinted inactivation of the paternal X. The inactive state of the Xp can be extensively studied ex vivo in Trophoblast Stem (TS) cells derived from the trophectoderm. We were able to select from the general cell population, TS cells exhibiting partial reactivation of the Xp or showing a complete switch of imprinted X-inactivation pattern. This reveals an accrued epigenetic plasticity of imprinted X-inactivation in the trophectoderm as compared to random X-inactivation in the soma.Random X-chromosome inactivation is recapitulated during the differentiation of female Embryonic Stem (ES) cells – which serves as cellular model. This process is triggered by the cis-accumulation of Xist long non coding RNA molecules which create a nuclear repressive domain around the future inactive X chromosome. Before differentiation, the accumulation of Xist is repressed by another lncRNA, Tsix, that is transcribed antisense to Xist. In order to address the functional dynamics of Xist and Tsix RNAs, we inserted different types of tag sequences in the endogenous Xist/Tsix locus. Incorporated in the sense or antisense RNA, these tags are specifically recognized by fluorescent molecules, thereby allowing live cell imaging of these transcripts.
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Investigação de microrrearranjos no cromossomo X pela técnica de MLPA em indivíduos do sexo masculino com deficiência intelectual de causa indeterminada / Investigation of microimbalances on the X chromosome by MLPA technique in male individuals with intellectual disability of unknown causesHenrique, Pamela Pontes, 1990- 30 August 2018 (has links)
Orientadores: Antonia Paula Marques de Faria, Maricilda Palandi de Mello / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-08-30T05:34:46Z (GMT). No. of bitstreams: 1
Henrique_PamelaPontes_M.pdf: 8320960 bytes, checksum: 1d8ff3e1253bf81cc18b4afd51572860 (MD5)
Previous issue date: 2015 / Resumo: A deficiência intelectual ligada ao X (DILX) é uma das causas genéticas mais frequentes de deficiência intelectual (DI), ocorrendo em 10 a 12% de todos os homens afetados, provavelmente pelo maior número de genes identificados no cromossomo X em comparação a qualquer segmento autossômico. Cerca de 100 genes seriam determinantes de DILX, porém mesmo com o conhecimento do papel de vários deles, há aspectos a serem elucidados, como a contribuição de cada um na determinação da DI ou ainda as correlações genótipo-fenótipo, cuja análise depende da investigação genética em indivíduos com DI idiopática. Entre os métodos que permitem a investigação molecular dessa condição destaca-se a Multiplex Ligation-dependent Probe Amplification (MLPA) por sua rapidez, sensibilidade e baixo custo. O objetivo do presente estudo foi investigar alterações em genes do cromossomo X pela técnica de MLPA em pacientes do sexo masculino com atraso global do desenvolvimento ou DI de origem indeterminada. Foram investigados 107 indivíduos com o kit SALSA MLPA P106 MRX probemix (MRC-Holland), 104 deles apresentaram resultado na faixa de normalidade e em três foram identificadas alterações do número de cópias interpretadas como duplicações. O paciente P13 apresentou alteração no gene HUWE1, que atua no controle da diferenciação neural e tem mutações descritas em algumas famílias com DI de moderada a grave; no paciente P139 foram identificadas alterações nos genes SCL6A8 e GDI, ambas confirmadas pela análise por Real Time Polymerase Chain Reaction (qPCR); mutações no primeiro são incluídas entre as síndromes de deficiência de creatina, com fenótipos variando de DI leve e atraso de fala até DI grave, convulsões e alterações de comportamento no sexo masculino, enquanto no segundo se associam à DILX inespecífica; já no paciente P39 foi detectada alteração no gene ARX, relacionado a mais de uma condição classificada como DILX sindrômica, que não foi confirmada. Como apenas alguns éxons relacionados à DILX foram investigados, não se afasta a eventual ocorrência de rearranjos localizados em regiões não abordadas pelo kit utilizado. Contudo, a técnica utilizada se mostrou uma opção de custo relativamente baixo e fácil reprodutibilidade, sendo viável para aplicação em algoritmos de investigação da DI. Os resultados reforçam a relevância da DILX entre as causas de DI, justificando a inclusão de testes moleculares específicos para a elucidação diagnóstica dessa condição / Abstract: X-linked intellectual disability (XLID) is one of the most frequent genetic causes of intellectual disability (ID), occurring in 10-12% of all affected men, probably because the larger number of identified genes on the X chromosome related to this condition than in any other autosomal segment. Although about 100 genes have been considered as determinant of XLID, the the role of several of these genes remains yet be elucidated despite the knowledge on the function of several of them. For instance, the contribution of each gene in determining the ID and the genotype-phenotype correlation depend on the genetic investigation of affected individuals. The Multiplex Ligation-dependent Probe Amplification (MLPA) is among the methods that allow molecular investigation of this condition because it is rapid and low cost and presents high sensitivity. The aim of this study was to investigate copy number variations in X-linked genes by MLPA technique in males with global developmental delay or ID of undetermined origin. A hundred and seven individuals were investigated using SALSA MLPA P106 MRX kit (MRC-Holland) and alterations were confirmed by Real Time Polymerase Chain Reaction (qPCR). A normal invariant pattern was observed in 104 out of 107 individuals, and three showed variations that have been interpreted as duplications. Patient P13 showed increased signal for HUWE1 gene, which plays a role in the control of neural differentiation. HUWE1 mutations have been described in families with moderate or severe ID. Patient P139 showed increased signals corresponding to regions of SCL6A8 and GDI1 genes. The former is included among genes involved in the creatine deficiency syndrome whose phenotype can range from mild ID and speech delay to severe ID, convulsions and behavior changes in males, and the latter is involved with non-syndromic XLID. Conversely, the variation in ARX gene, which is associated to more than one condition classified as syndromic XLID, observed in MLPA analysis for patient P39 was not confirmed in the qPCR assay. As only a few exons related to XLID were investigated, it does not rule out the possible occurrence of rearrangements located in regions not covered by the kit used. However, the technique employed was an easily reproducible, relatively low cost option, manageable for application in ID research algorithms. The results reinforce the importance of XLID among the causes of ID, justifying the inclusion of specific molecular tests for the laboratory diagnosis of this condition / Mestrado / Ciencias Biomedicas / Mestra em Ciências Médicas
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Estudo do padrão de inativação do cromossomo X em tecido extra-embrionário humano / X-chromosome inactivation pattern in human extra-embryonic tissueJoana Carvalho Moreira de Mello 08 April 2010 (has links)
Em mamíferos a inativação do cromossomo X (ICX) consiste no silenciamento gênico de um dos dois X presentes nas células somáticas normais das fêmeas, garantindo a compensação de dose transcricional em relação aos machos. Existem duas formas de ICX: aleatória, na qual a escolha do cromossomo X inativado se dá ao acaso (X paterno ou materno); e de maneira completamente desviada, na qual a atividade do cromossomo X dependerá de sua origem parental. Nas fêmeas marsupiais a inativação ocorre de forma completamente desviada, sendo o X paterno preferencialmente inativado em todas as células, já nas células embrionárias de eutérios, o que se observa é a ICX aleatória. Entretanto, naquelas células que darão origem aos tecidos extra-embrionários, de camundongos e bovinos, a ICX se dá de forma equivalente à dos marsupiais, ou seja, o X paterno é preferencialmente inativado. Há mais de 30 anos o padrão de ICX em tecidos extra-embrionários humanos tem sido alvo de intenso debate. A crítica que se faz aqui é que tais estudos foram realizados com base na expressão de apenas um ou dois genes ligados ao X com amostras de tecidos extra-embrionários em diferentes idades gestacionais e, por vezes, em poucas amostras, o que deve ter levado às contradições entre as conclusões. O diferencial deste trabalho foi a utilização de técnicas de genotipagem de SNPs presentes em regiões codificadoras, para analisar o padrão de atividade alelo-específica de um grande número de genes presentes ao longo de todo o cromossomo X, gerando um panorama mais representativo da ICX em placenta humana. Neste estudo é comprovado o padrão aleatório de ICX em placenta humana a termo e demonstrado que este órgão se apresenta como um 65 mosaico em relação à escolha do X inativo. A análise global da atividade gênica no cromossomo X indicou ainda que a manutenção do estado epigenético do X inativo parece ser heterogêneo. Em conjunto, os dados gerados são capazes de explicar as incongruências entre as conclusões previamente publicadas. Este trabalho também ilustra as diferenças nos mecanismos de ICX entre humanos e camundongos e reforça a importância de se avaliar esse tema em outras espécies de mamíferos eutérios na tentativa de se elucidar os processos evolutivos envolvidos na compensação de dose em mamíferos / Imprinted inactivation of the paternal X chromosome in marsupials is the primordial mechanism of dosage compensation for X-linked genes between females and males in Therians. In Eutherian mammals, X chromosome inactivation (XCI) evolved into a random process in cells from the embryo proper, where either the maternal or paternal X can be inactivated. However, species like mouse and bovine maintained imprinted XCI exclusively in extraembryonic tissues. The existence of imprinted XCI in humans remains controversial, with studies based on the analyses of only one or two X-linked genes in different extraembryonic tissues. Here we readdress this issue in human term placenta by performing a robust analysis of allele-specific expression of 23 X-linked genes, including XIST, using 28 SNPs in transcribed regions. We show that XCI is random in human placenta, and that this organ is arranged in relatively large patches of cells with either maternal or paternal inactive X. In addition, this chromosome-wide analysis indicated heterogeneous maintenance of the epigenetic state along the inactive X, which combined with the extensive mosaicism found in placenta, can explain the lack of agreement among previous studies. Our results illustrate the differences of XCI mechanism between humans and mice, and highlight the importance of addressing the issue of imprinted XCI in other species in order to understand the evolution of dosage compensation in placental mammals
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Ki-67 Regulates Cell Cycle Progression and Heterochromatin OrganizationSun, Xiaoming 15 September 2017 (has links)
A subset of eukaryotic heterochromatin is located around the nucleoli, and this localization is correlated with gene silencing. Although there is some evidence for trans-acting factors organizing genomic loci around the nucleolus, the characterization of proteins and /or RNAs involved in perinculeolar heterochromatin localization and maintenance is incomplete. Notably, the mammalian female inactive X chromosome, a well-studied model of facultative heterochromatin, frequently resides in the perinucleolar regions during mid to late S phase. The disruption of the Xi–nucleolus association results in the erosion of heterochromatin compartment and silencing, which renders it a good model to investigate the mechanism and biological relevance of heterochromatin organization around the nucleolus. This dissertation will present evidence showing that Ki-67 regulates inactive X (Xi) chromosome association with nucleoli, maintains Xi heterochromatic structures, and regulates cell cycle progression, in cell-type-specific manner dependent on checkpoint proficiency.
Ki-67 protein plays roles in heterochromatin organization during interphase. Upon Ki-67 depletion, a subset of Xi in human female hTERT-RPE1 moved away from nucleolus and displayed several features of compromised heterochromatin maintenance. These chromatin alterations were limited to Xi chromosomes localized away from the nuclear lamina and were not observed in virally transformed 293T cells upon Ki-67 depletion. Furthermore, I demonstrated that the different Xi heterochromatin alteration responses result from cell-type-specific reduced proportion of cells in S phase upon Ki-67 depletion. In human hTERT-RPE1, WI-38, IMR90, hTERT-BJ cell lines, depletion of Ki-67 slowed entry into S phase and coordinately downregulated genes related to DNA replication. These cell lines are able to induce p21 expression upon Ki-67 depletion. On the contrary, alteration of transcription and cell cycle progression were not observed in tumor-derived HeLa, U2OS and 293T cell lines. These cell lines do not induce p21 expression either. I additionally examined the Ki-67 function in mouse cell cultures. Depletion of Ki-67 neither redistributes inactive X chromosome nor regulates S phase progression in primary female mouse embryonic cells.
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Differential Sexual Survival of <i>D. Melanogaster </i> on Copper SulfateBalinski, Michael A. 14 July 2016 (has links)
No description available.
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