Objective: To describe the prevalence of dengue virus serotypes, as well as other viral and bacterial pathogens that cause acute febrile illness during an outbreak in Cajamarca in 2016. Results: Dengue virus (DENV) was the most frequent etiologic agent detected in 25.8% of samples (32/124), followed by Rickettsia spp. in 8.1% (10/124), Zika virus in 4.8% (6/124), Chikungunya virus 2.4% (3/124) and Bartonella bacilliformis 1.6% (2/124) cases. No positive cases were detected of Oropouche virus and Leptospira spp. DENV serotypes identification was only achieved in 23% of the total positive for DENV, two samples for DENV-2 and four samples for DENV-4. During the 2016 outbreak in Cajamarca-Peru, it was observed that in a large percentage of positive samples for DENV, the infecting serotype could not be determined by conventional detection assays. This represents a problem for the national surveillance system and for public health due to its epidemiological and clinical implications. Other viral and bacterial pathogens responsible for acute febrile syndrome were less frequently identified. / Revisión por pares

archives@tulane.edu / Immune signatures are patterns of gene and protein expression in immune cells that characterize states of activation and response. As such, signatures indicative of viral control during natural infection may guide vaccine development efforts to achieve similar patterns of protection. Here, we used nonhuman primate (NHP) models of Zika virus (ZIKV) and simian immunodeficiency virus (SIV, as a model for HIV) to explore outcomes of infection in these important human pathogens. We employed a multifaceted approach including high dimensional flow cytometry and RNA sequencing to understand cellular responses to ZIKV generally and during pregnancy, as well as to identify the impacts of infection in astrocytes, a neuroglial target of ZIKV thought to be important in the development of neurologic disease. We found that CD8 T cells may restrict ZIKV persistence in tissues but ultimately have a minimal role in protection to either primary or secondary challenge. However, we showed that immune manipulation, either naturally through pregnancy or artificially through depletion experiments, can skew metabolic and innate immune pathways in unexpected ways. While cellular immunity appeared to minimally impact ZIKV infection, such responses in SIV are important in controlling viral replication, which we inversely showed by tracking patterns of viral mutation to evade CD8 responses. We also identified transcriptional signatures in ZIKV infection that may underlie the development of neurologic diseases and found that different virus lineages have unique impacts on gene expression. Together, these experiments showcase the utility of profiling approaches in understanding the immune complexity that accompanies viral infection. / 1 / Blake Schouest

No description available.

Le virus Zika est un pathogène qui a récemment retenu l’attention de la communauté scientifique suite à une importante éclosion en Amérique du Sud. Ce virus, peu étudié auparavant, peut causer des pathologies parfois graves chez les personnes infectées. Nous avons démontré que le virus Zika peut infecter les macrophages dérivés de monocytes. Plusieurs souches virales peuvent infecter les macrophages, mais certaines différences existent entre ces souches quant au taux d’infection et la cinétique de réplication. La réplication virale serait localisée autour du noyau de la cellule, comme ce qui est connu des autres membres de la famille des Flaviviridae. Les macrophages sont résistants aux effets cytopathiques du virus et peuvent donc jouer un rôle de réservoir viral à long terme. L’autophagie est un mécanisme nécessaire et important à la complétion du cycle viral du virus Zika dans les macrophages. Lors d’une co-infection entre le virus Zika et le VIH-1 dans les macrophages, il n’y a pas d’effet observé sur le taux d’infection et la réplication virale du VIH-1. Cependant, il y a une diminution de la réplication virale du virus Zika lors de ces co-infections. L’ajout de B18R, un inhibiteur de la réponse interféron de type I vient contrecarrer cette modulation, démontrant le mécanisme d’action de cette modulation. L’ajout d’un inhibiteur de la signalisation de la voie de l’interféron permet d’augmenter le taux d’infection des macrophages par le virus Zika. En résumé, le virus Zika peut infecter les macrophages et ces cellules pourraient être un réservoir viral à long terme, le virus a besoin de l’autophagie pour se répliquer et la production d’interféron de type I par les cellules qui sont infectées par un autre virus a un effet important sur la diminution de la réplication du virus Zika. / Zika virus is a pathogenic agent who recently gathered attention from the scientific community following a major outbreak in South America. This virus, who received little attention prior to this outbreak, can induce major pathologies in infected individuals. Our results indicate that Zika virus can infect monocyte-derived macrophages. Many viral strains can infect macrophages, but some differences were put in evidence, particularly regarding the infection rate and replication kinetic of the different strains. Viral replication occurs around the nucleus, which is expected and known from other viruses of the Flaviviridae family. Macrophages are resistant to the cytopathic effect of the virus, and can produce virus for many days. Autophagy is a necessary, and important cellular mechanism in completion of the virus life cycle. In the context of confections with Zika virus and HIV1, there is no effect observed in the infection rate and replication level of HIV-1. However, there is a decrease observed in the overall replication of Zika virus. The presence of B18R, a type I interferon inhibitor, restores a normal viral replication, demonstrating the mechanism behind this modulation. Addition of an inhibitor targeting the interferonmediated signalling pathway increases the infection rate of macrophages by Zika virus. In summary, Zika virus can infect macrophages, requires the autophagy signalling pathway to successfully replicate in macrophages, and is negatively impacted by interferon produced by its host cells infected by another virus.

INTRODUÇÃO: Houve uma epidemia de infecção pelo vírus Zika (ZIKV) no Brasil, entre 2015 e 2016, que refletiu no aumento de casos de microcefalia neonatal secundária à infecção congênita pelo ZIKV. Para compreensão do padrão de acometimento neurológico dessas crianças foi realizado um projeto de pesquisa pelo Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (HCFMRP-USP), conhecido como ZIG, e realizado seguimento neurológico desses pacientes por meio do Exame Neurológico de Hammersmith Neonatal (HINE-N). OBJETIVOS: Avaliar pacientes com microcefalia secundária à infecção congênita pelo ZIKV por meio do HINE- N, descrever as características clínicas e de neuroimagem dos pacientes estudados e identificar fatores pré-natais, perinatais e demográficos potencialmente relacionados ao grau de comprometimento do exame neurológico. METODOLOGIA: O presente estudo faz parte do Núcleo de Estudos sobre Infecção Materna, Perinatal e Infantil (NEIMPI) do HCFMRP-USP e trata-se de um estudo não experimental, transversal, com intuito de correlacionar variáveis qualitativas e quantitativas. Participaram do estudo crianças com microcefalia secundária à infecção congênita pelo ZKV nascidas no município de Ribeirão Preto e seu Departamento Regional de Saúde (DRS XIII), no período de outubro de 2015 a dezembro de 2016. Os dados foram coletados no período de outubro de 2015 a janeiro de 2017. Realizou-se avaliação do perímetro craniano (PC), aplicação do HINE e coleta de dados em prontuário. Os dados foram processados e analisados com a utilização do Pacote Estatístico Statistical Package for the Social Sciences (SPSS), versão 22.0. RESULTADOS: Foram avaliadas 18 crianças, sendo predomínio do sexo feminino (55,6%), nascidas a termo, com exceção de uma que nasceu com 32 semanas, maioria originária de Ribeirão Preto (66,7%), via de parto normal (72,2%), ocorrido no Centro Obstétrico do HCFMRP - USP (83,3%), todas as crianças tiveram APGAR maior que 7. O peso de nascimento foi entre 1228 e 3200 gramas, com PC entre 26 e 31,5 cm. 72,2% das crianças fizeram ultrassom transfontanela e 100% fizeram ressonância magnética de encéfalo, com associação entre os resultados encontrados neles (ambos com p<ou=0,001). O score HINE-N foi baixo, porém não foram encontradas associações entre o score e as variáveis clínicas (ambos com p>0,05). Evidenciou-se associação entre a realização de pré-natal e a cidade de residência, local de nascimento, diagnóstico de microcefalia e de infecção pelo ZIKV no pré-natal, tipo de parto (p< ou = 0,001). A medida do PC apresentou associação direta com a presença de crise convulsiva (p<0,05) e houve associação entre crise convulsiva, ventriculomegalia e calcificação (p< ou= 0,001). CONCLUSÃO: O padrão de acometimento neurológico das crianças com microcefalia secundária à infecção congênita pelo ZIKV é muito severo, com presença de sinais precoces de paralisia cerebral desde o exame neonatal. Este estudo sugere seguimento prospectivo desses pacientes para melhor avaliação prognóstica e associação do score HINE com condições clínicas pré e perinatais / INTRODUCTION: There was an epidemic of Zika virus infection (ZIKV) in Brazil between 2015 and 2016, which reflected the increase in cases of neonatal microcephaly secondary to congenital ZIKV infection. A study was carried out for the Hospital das Clínicas of the University of São Paulo at Ribeirão Preto Medical School (HCFMRP-USP), and the followup of these patients was performed using the Neonatal Hammersmith Neurological Exam (HINE-N) aiming to understand the neurological involvement pattern of these children. OBJECTIVES: To evaluate patients with microcephaly secondary to ZIKV congenital infection using HINE-N, to describe the clinical and neuroimaging characteristics of the patients studied, and to identify prenatal, perinatal and demographic factors potentially related to the degree of impairment of neurological examination. METHODOLOGY: This study is part of the Center for Studies on Maternal, Perinatal and Child Infection (NEIMPI) of the HCFMRP-USP. It is a non-experimental, cross-sectional study aimed at correlating qualitative and quantitative variables. Children with microcephaly secondary to congenital ZKV infection born in the city of Ribeirão Preto and its Regional Health Department (DRS XIII), from October 2015 to December 2016, participated in the study. Data were collected in the period of October 2015 to January 2017. An evaluation of the cranial perimeter (PC), the application of HINE and data collection in medical records were performed. Data were processed and analyzed using the Statistical Package for Social Statistical Package (SPSS), version 22.0. RESULTS: A total of 18 children were evaluated, being 55,6% female, all to term with the exception of one who was born at 32 weeks, most of them from Ribeirão Preto (66.7%), normal delivery (72.2%), occurred in the Obstetric Center of the HCFMRP - USP (83.3%), all children had APGAR greater than 7. The birth weight was between 1228 and 3200 grams, with PC between 26 and 31.5 cm. 72.2% of the children had transfontanel ultrasonography and 100% had brain magnetic resonance imaging, with an association between the results found in them (both with p <or = 0.001). The HINE-N score was low, but no associations were found between the score and the clinical variables (both with p> 0.05). It was evidenced an association between prenatal and city of residence, place of birth, diagnosis of microcephaly and ZIKV infection in the prenatal period, type of delivery (p <or = 0.001). The PC measurement presented a direct association with the presence of seizures (p <0.05) and there was an association between seizure, ventriculomegaly and calcification (p <or = 0.001). CONCLUSION: The pattern of neurological involvement of children with microcephaly secondary to congenital ZIKV infection is very severe, with early signs of cerebral palsy since the neonatal examination. This study suggests a prospective follow-up of these patients for better prognostic evaluation and association of the HINE score with pre and perinatal clinical conditions

Zika vírus (ZIKV) é um arbovírus que ganhou grande relevância nos últimos anos. Zika vírus (ZIKV) é um arbovírus que ganhou grande relevância nos últimos anos. Zika Vírus (ZIKV) é um arbovírus que ganhou grande relevância nos últimos anos. Embora sua infecção geralmente induza sintomas leves, em alguns casos, a está associada à microcefalia em recém-nascidos e síndrome de Guillain-Barré em adultos. RNAs longos não codificadores (lncRNAs) desempenham um papel fundamental na modulação do sistema imunológico, mas pouco se sabe sobre sua função na infecção aguda por ZIKV. Portanto, o objetivo deste projeto foi estudar o transcritoma de macacos Rhesus durante a infecção e como os lncRNAs podem alterar a resposta em diferentes mamíferos infectados por ZIKV. Foram infectados quatro macacos Rhesus com a cepa HS-2015-BA-01 e o sangue foi coletado antes e depois de 1, 3, 5, 7, 10 e 14 dias de infecção. Em seguida, foi realizado o RNA-seq com amostras de sangue total para avaliar as alterações transcricionais durante a infecção. Nossas análises revelaram uma regulação da resposta imune, incluindo a identificação de lncRNAs que foram induzidos na infecção por ZIKV. Identificamos cerca de 9,210 lncRNAs, dos quais 3,246 ainda não foram anotados na base de dados ENSEMBL e 140 deles foram diferencialmente expressos em alguma comparação. Alguns genes associados à resposta imune, como STAT1, JAK1 e IFNGR2, parecem ser regulados negativamente. Análises de módulos de co-expressão e de redes revelaram as possíveis vias e genes afetados por alguns lncRNAs identificados. Os lncRNAs PROAP1, 2 e 3, ainda não depositados em bancos públicos, foram altamente correlacionados com BCL2, CYBA e MYCT1, que podem potencialmente regular a resposta pró-apoptótica. Além disso, outros conjuntos de dados (camundongos, neonatos e cultura celular) foram utilizados para avaliar a importância e a interferência dos lncRNAs na infecção. Com este estudo, encontramos lncRNAs previamente não descritos no genoma de M. mulatta, alguns dos quais estão correlacionados com genes importantes. Comparando todos os resultados, percebemos que alguns lncRNAs podem desempenhar papéis semelhantes, ainda que em diferentes animais. No geral, nossos resultados sugerem que a infecção por ZIKV modifica a expressão de genes codificadores e lncRNAs, quanto à mecanismos apoptóticos de resposta contra o vírus. / Zika Virus (ZIKV) is an arbovirus that has gained high relevance in recent years. Although ZIKV infection generally induces mild symptoms, in some cases, the infection is associated to microcephaly in newborns and Guillain-Barré syndrome in adults. Long non-coding RNAs (lncRNAs) play a key role in modulating the immune system but nothing is known about their function in acute ZIKV infection. Therefore, the aim of this project was to study the transcriptome of infected Rhesus monkeys throughout the infection and how lncRNAs can alter the response in different mammals infected by ZIKV. We had infected four Rhesus monkeys with HS-2015-BA-01 strain and collected their blood before and after 1, 3, 5, 7, 10 and 14 days of infection. We then performed RNA-seq with whole blood samples to assess the transcriptional changes during infection. Our systems biology analyses revealed a regulation of immune response during the infection including the identification of many lncRNAs that were induced upon ZIKV infection. We identified around 9.210 lncRNAs, 3.246 of which werent annotated on the ENSEMBL database, and 140 of which were differentially expressed in some comparison. Some genes associated with immune response such as STAT1, JAK1 and IFNGR2, appear to be negatively regulated. Co-expression modules and network analyses have revealed the putative pathways and genes affected by these lncRNAs. Such as PROAP1, 2 and 3, not yet deposited in public banks, were highly correlated with BCL2, CYBA and MYCT1, that can potentially regulate pro-apoptotic response. In addition, other datasets (mice, neonates an cell culture) were used to further assess the importance and interference of lncRNAs in the infection. With this study we found many previously undescribed lncRNAs in the M. mulatta genome, some of which are correlated with important genes. Comparing all these results we found that some lncRNAs may play similar roles although in different animals. Overall, our results suggest that ZIKV infection modifies the expression of coding genes and lncRNAs that lead to apoptotic mechanisms against the virus.

A febre Zika é uma enfermidade que afeta pessoas de países tropicais e subtropicais. O agente etiológico da doença é o vírus Zika (ZIKV), um flavivirus de genoma RNA de fita simples, transmitido principalmente por mosquitos do gênero Aedes. Devido a associação do ZIKV à microcefalia e síndrome de Guillain-Barré é necessário desenvolver estratégias para a prevenção da doença. Entre eles, a melhor medida profilática é a vacinação. A proteína NS3 tem sido relatada como um potencial ativador da imunidade celular contra o ZIKV e outros flavivírus, cuja atuação tem sido utilizada como um alvo contra infecções virais. Neste contexto, o objetivo deste projeto foi testar a eficiência da NS3 como ativadora da resposta imunológica e, em combinação com o vírus inativado, produzir uma vacina capaz de desenvolver proteção total contra a infecção viral. Uma sequência da NS3 foi clonada no vetor pET-26b, expresso em E. coli Rosetta e purificado por cromatografia de afinidade em coluna de níquel. A completa inativação do ZIKV foi realizada por meio de adição de formaldeído 0,05% e incubação por 3 dias. Combinações vacinais de NS3 e vírus inativado foram inoculados em vários grupos de camundongos 129 Sv/Ev e A129 nos dias 0 e 14. No dia 21, os camundongos A129 vacinados foram desafiados com o ZIKV selvagem e analisados segundo a perda de peso e sobrevivência por 21 dias. Os camundongos vacinados com NS3 e vírus inativado, na mesma formulação vacinal, tiveram 100% de proteção contra o ZIKV infeccioso. O sangue total e o baço foram coletados dos camundongos 129 Sv/Ev e A129 após 21 dias de uma nova imunização. Foi avaliado a produção de anticorpos específicos e neutralizantes contra ZIKV por ELISA e PRNT, mostrando que animais imunizados com a formulação vacinal \"vírus inativado com NS3\" produzem mais anticorpos contra o vírus, porém não foi detectado neutralização do vírus em nenhum grupo imunizado. O perfil de citocinas expressas por linfócitos do baço, analisado por FACS, sugere que a NS3 participa na modulação para uma resposta Th1. Esta abordagem possibilitou a avaliação de uma combinação vacinal que se mostrou capaz de prevenir a infecção com o ZIKV em camundongos susceptíveis / Zika Fever is a disease that affects many people in tropical and subtropical countries. The etiologic agent is Zika Virus (ZIKV), a single-stranded RNA flavivirus, transmitted mostly by Aedes mosquitoes, but sexual, congenital and blood transfusion transmission has also been reported. Due to the possible association of ZIKV to microcephaly and Guillain-Barré Syndrome, it is necessary to develop strategies for disease prevention. Among them, the best prophylactic measure is vaccination. The non-structural protein NS3 has been shown to stimulate cellular immunity response against others flaviviruses, and such activity has been used as a target against other flaviviruses infection. In this context, the aim of this project is to test the efficiency of NS3, in combination with the inactivated virus, to produce a vaccine capable of developing full protection against viral infection. The NS3 sequence was cloned into the pET-26b vector, expressed in E. coli Rosetta and purified by nickel column affinity chromatography. The complete inactivation of ZIKV was performed by addition of 0.05% formaldehyde and incubation for 3 days. Vaccine combinations of NS3 and inactivated virus were inoculated into 129 Sv/Ev and A129 mice on days 0 and 14. On day 21, vaccinated A129 mice were challenged with wild ZIKV and analyzed for weight loss and survival by 21 days. Mice vaccinated with NS3 and inactivated virus, in the same vaccine formulation, had 100% protection against infectious ZIKV. Whole blood and spleen cells were collected from 129 Sv/Ev and A129 mice after 21 days of new immunization. The production of specific and neutralizing antibodies against ZIKV was evaluated by Imunnofluorescence, ELISA and PRNT, showing that animals immunized with the vaccine formulation \"virus inactivated with NS3\" increased the antibodies\' level against the virus, but no virus neutralization was detected in any immunized group. The cytokine profile expressed by spleen lymphocytes, analyzed by FACS, suggests that NS3 participates in modulation for a Th1 response. This approach gave us the opportunity to evaluate this vaccinal combination that was shown to be able to prevent infection with ZIKV in susceptible mice

No Brasil a confirmação do primeiro caso de febre zika, resultante da infecção pelo vírus zika (ZIKV), foi no primeiro semestre de 2015, na região nordeste. Os achados característicos eram de uma doença que apresentava sintomas semelhantes aos sintomas causados pelo vírus dengue (DENV), porém mais amenos, sendo inicialmente denominada de \"Síndrome Dengue-Like\". Além desta semelhança no quadro clínico, ZIKV e os DENV são arboviroses endêmicas no Brasil e pertencem à mesma família viral, sendo muito próximos filogeneticamente, resultando em uma forte reação cruzada entre os anticorpos induzidos por estas infecções. O diagnóstico específico para o ZIKV requer cuidados uma vez que o DENV circula há muito mais tempo no Brasil e muitas pessoas já são imunes para, no mínimo, um sorotipo da doença. Desta forma, anticorpos contra os DENV nos testes sorológicos podem reagir inespecificamente contra o ZIKV, originando um resultado falso-positivo. As maneiras para diferenciar as duas doenças são o isolamento e a detecção do RNA viral durante a fase aguda da doença e dos anticorpos neutralizantes durante a fase de convalescença. Atualmente com a escassez de testes registrados pela ANVISA e a dificuldade em diferenciar as duas viroses usando testes sorológicos, amostras bem caracterizadas e confirmadas quanto à infecção pelo ZIKV foram utilizadas para o teste de comparação com vários kits comerciais aprovados ou não pela ANVISA, para detecção de anticorpos. Estas amostras também foram testadas por um ensaio soro-molecular padronizado e desenvolvido no Laboratório de Virologia Molecular. / In Brazil, the first case of zika fever, resulting from zika virus (ZIKV) infection, was confirmed in the first half of 2015, in the northeast region. The characteristic findings were of a disease that presented symptoms similar to the symptoms caused by dengue viruses (DENV), but milder, being initially called \"Dengue-Like Syndrome\". Although this similarity in the clinical presentation, ZIKV and (DENV) are arboviruses endemic in Brazil and belonging to the same viral family, being very close phylogenetically, and resulting in a strong cross-reaction between the antibodies induced by these infections. The specific diagnosis for ZIKV requires careful evaluation since DENV has been circulating much longer in Brazil and many people are already immune to at least one serotype of the disease. Thus, the antibodies against DENV may react non-specifically against ZIKV in serological tests, resulting in a false-positive result. The ways to differentiate the two diseases are the isolation and detection of viral RNA during the acute phase of the disease and the neutralizing antibodies during the convalescent phase. Actually, with the scarcity of tests registered by ANVISA and the difficulty in differentiating the two viruses using serological tests, well-characterized and confirmed samples for ZIKV infection were used for the comparison of several commercial kits for detection of antibodies approved or not by ANVISA. These samples were tested by a standardized serummolecular assay developed at the Molecular Virology Laboratory.

Le virus Zika (ZIKV) est un phénomène épidémiologique sans précédent qui surprit le monde entier. Pendant de nombreuses années, il fut considéré comme un virus anodin responsable d’une poignée d’infections humaines, auto-limitées et bénignes, en Afrique et en Asie du Sud-est. Mais, après des décennies de propagation silencieuse, une première épidémie éclata en Micronésie en 2007 - tel un signal d'alarme. Quelques années plus tard, une soudaine épidémie de ZIKV de plus grande ampleur se déclara dans les îles du Pacifique avant d'atteindre le Brésil en 2015. Au cours de cette période, Zika fut associé à de graves complications neurologiques, mettant en évidence son fort potentiel pathogène pour l'homme. Depuis son émergence, plus de 80 pays et territoires ont été touchés par la pandémie de ZIKV, désormais reconnu comme un virus neurotrope et tératogène. L'association des souches contemporaines de ZIKV à des formes graves de maladie chez l'homme, qui n'ont jamais été signalées auparavant, a soulevé l'hypothèse d'une pathogénicité nouvellement acquise. Ainsi, mes travaux de doctorat visaient à déterminer si l'ampleur de l'épidémie actuelle pouvait en partie avoir été facilitée par des facteurs viraux qui auraient renforcé la fitness du ZIKV. À cette fin, mon projet de recherche s'est concentré sur l'identification des facteurs moléculaires viraux impliqués dans la pathogénicité du virus Zika chez l’homme à partir du développement de clones moléculaires. / Zika virus (ZIKV) is an unprecedented epidemiological phenomenon which surprised the world. For many years, it was considered a trivial virus responsible for only a handful of human infections, self-limited and benign, in Africa and Southeast Asia. But then, after decades of silent spread, a first epidemic broke out in Micronesia in 2007 – like a warning signal. A few years later, a sudden Zika outbreak of larger scale occurred in the Pacific islands before reaching Brazil in 2015. During this period, Zika was associated with severe neurological complications, highlighting its serious pathogenic potential for humans. Since its emergence, more than 80 countries and territories have been affected by the ZIKV pandemic, which is now recognized as a neurotropic and teratogenic virus. The association of contemporary ZIKV strains with severe forms of disease in humans, that have never been reported before, has raised the hypothesis of newly acquired pathogenicity. In this regard, my doctoral research aimed to determine whether the scope of the current epidemic was partly facilitated by viral factors that improved ZIKV fitness. To this end, my research project focused on the identification of the viral molecular factors involved in Zika virus pathogenicity in humans based on the development of molecular clones.

Introdução: Em 2015, a infecção pelo zika começou a chamar a atenção de profissionais de saúde da região Nordeste. Apesar dos sintomas semelhantes aos da dengue, tratava-se de uma enfermidade que até então não estivera presente no território brasileiro. No segundo semestre de 2015, houve um aumento nos casos de microcefalia em recém-nascidos e com isso, pesquisadores descobriram que essa condição neurológica estava associada à infecção pelo referido vírus em gestantes. Desta forma, a gravidez em tempos de zika tornou-se uma preocupação para mulheres. Vivenciar uma gestação nestas condições poderia resultar em sofrimento mental para as mulheres, principalmente ao considerar que as áreas mais afetadas pela epidemia são marcadas por vulnerabilidades econômicas e sociais. Grupos e instituições que defendem o direito ao aborto nos casos de infecção pelo zika colocam seus argumentos em circulação na esfera pública, ao mesmo tempo em que atores com posicionamento contrário procuram defender seus discursos. Objetivos: Buscou-se mapear a controvérsia em torno do zika e a interrupção da gravidez para gestantes infectadas pelo vírus em dois jornais brasileiros, Folha de S. Paulo e O Estado de S. Paulo. Metodologia: Trata-se de uma pesquisa qualitativa, envolvendo análise documental de notícias. Foram selecionados textos jornalísticos do período de novembro de 2015 a dezembro de 2017. Por meio da Teoria Ator-Rede de Latour, buscou-se mapear a controvérsia sobre o direito ao aborto para gestantes infectadas pelo zika, identificando os atores e os argumentos que a compõem, por meio da elaboração de categorias de análise. Resultados e discussão: Observou-se que os atores que se posicionaram diante do problema foram médicos, pesquisadores, representantes da esfera jurídica, representantes governamentais, movimentos sociais, organismos internacionais e grupos religiosos. Os argumentos favoráveis recorrentes foram a gravidade da microcefalia, negligência do Estado, sofrimento das mulheres atingidas pela epidemia, defesa da descriminalização do aborto. Enquanto que os argumentos contrários se concentraram na incerteza sobre a relação causal entre zika e microcefalia e o valor da vida humana. Além disso, a presença de vozes das mulheres afetadas pela epidemia foi menor em relação aos outros atores. Considerações finais: O debate sobre a relação entre o vírus e o direito ao aborto foi perdendo força após o ano de 2016, porém reacendeu o debate do aborto. O zika pode ser um objeto adequado para refletir como a comunidade médica e de pesquisadores constroem controvérsias nos veículos midiáticos, bem como outros atores envolvidos no tema, como movimentos sociais, grupos religiosos, representantes governamentais, jurídicos, organismos internacionais. No entanto, nos veículos de comunicação, há uma hegemonia do discurso científico, enquanto que as vozes das mulheres não ecoam neste meio. / Introduction: In 2015, the zika infection began to catch the attention of health professionals in the Northeast region. Despite the symptoms similar to those of dengue, it was a disease that had not been present in Brazil until then. In the second half of 2015, there was an increase in the cases of microcephaly in newborns and with that, researchers found that this neurological condition was associated with the infection by said virus in pregnant women. In this way, pregnancy in times of zika has become a concern for women. Experiencing a pregnancy in these conditions could result in mental suffering for women, especially considering that the areas most affected by the epidemic are marked by economic and social vulnerabilities. Groups and institutions that defend the right to abortion in cases of zika infection place their arguments in the public sphere, while opposing actors try to defend their speeches. Objectives: We aimed to map the controversy around zika and the interruption of pregnancy to pregnant women infected by the virus in two Brazilian newspapers, Folha de S. Paulo and O Estado de S. Paulo. Methodology: This is a qualitative research, involving documentary analysis of news. Journalistic texts were selected from November 2015 to December 2017. Through the Latour Actor-Network Theory, we tried to map the controversy over the right to abortion for pregnant women infected with zika, identifying the actors and the arguments that the compose, through the elaboration of categories of analysis. Results and discussion: It was observed that the actors who posed the problem were doctors, researchers, legal representatives, government representatives, social movements, international organizations and religious groups. Favorable arguments were the seriousness of microcephaly, neglect of the State, the suffering of women affected by the epidemic, and the defense of the decriminalization of abortion. While the opposing arguments focused on uncertainty about the causal relationship between zika and microcephaly and the value of human life. In addition, the voices of the women affected by the epidemic were lower than the other actors. Final Considerations: The debate over the relationship between the virus and the right to abortion was losing strength after 2016, but it rekindled the abortion debate. The zika can be an appropriate object to reflect how the medical community and researchers construct controversies in the media vehicles, as well as other actors involved in the theme, such as social movements, religious groups, government representatives, jurists, international organizations. However, in the media, there is a hegemony of scientific discourse, while the voices of women do not echo in media vehicles.