L'interférence ARN est un ensemble de mécanismes de régulation essentiel pour denombreux processus au cours du développement. Ces mécanismes sont caractérisés par lʼinhibition séquence-spécifique de lʼexpression de gènes via lʼaction de petites molécules dʼARN. Parmi les différentes voies de l'interférence ARN, la voie des trans-acting siRNAs dérivés du précurseurTAS3,qui combine des caractéristiques des voies des miRNAs et siRNAs, est spécifique des plantes et en contrôle divers aspects essentiels du développement. Dans cette voie, AGO7, un membre de la famille des protéines ARGONAUTE, interagit spécifiquement avec miR390 pour cibler et cliver le transcrit TAS3 amorçant ainsi la production de tasiARFs par lʼaction de SGS3, RDR6 et DCL4. Cette voie est conservée chez toutes les plantes terrestres. Par leur activité de répression sur des membres des facteurs de réponse à l'auxine ARF2, ARF3 et ARF4, les tasiARFS contrôlent la transition de phase et la régionalisation des feuilles. Notre laboratoire et dʼautres ont montré que TAS3 était également exprimé dans la racine dʼArabidopsis, posant la question du rôle joué par la voie TAS3dans le développement des racines. Nous avons établi que la voie TAS3, par lʼaction des tasiARFs, joue un rôle essentiel dans le contrôle de la croissance des racines latérales. Nous avons démontré que la voie TAS3 agit en aval de lʼauxine pour maintenir la correcte zone et abondance des facteurs de réponse à l'auxine, ARF2, ARF3 et ARF4. De plus nous avons élucidé un ensemble complexe de rétroactions de ces ARFs sur lʼexpression de miR390. Bien que les mécanismes de la voie TAS3 ont été identifié par divers screen génétiques, notre connaissance de lʼorganisation subcellulaire de cette voie reste essentiellement méconnue. Pour cette raison, nous avons choisi dʼétudier la localisation subcellulaire de la voie TAS3, en nous focalisant sur AGO7 qui en constitue un composant spécifique. Nous avons montré que AGO7, RDR6 et SGS3 sʼaccumulent dans des focicytoplasmiques spécifiques, les siRNA bodies. Nous avons observé une colocalisation entre cessiRNA bodies et des marqueurs des stress granules ainsi qu'une protéine virale associée aux membranes. Finalement nous avons démontré lʼimportance de la localisation cytoplasmique dʼAGO7pour la biogenèse des tasiARFs. Notre travail a permis de mieux comprendre les mécanismes de lʼaction de la voie TAS3 au cours du développement dʼArabidopsis. / RNA silencing is a regulatory mechanism essential for many processes during development. This mechanism is characterized by the sequence-specific inhibition of gene expression by small RNA molecules. Among the several pathways of RNA silencing, the TAS3 trans-acting small interfering RNA (ta-siRNA) pathway, which combines features of both micro (mi)RNA and siRNA pathways, is unique to plants and controls several key aspects of plant development. In this pathwayAGO7, a member of the ARGONAUTE family of RNAse, interacts specifically with miR390 to target and cut the TAS3 transcript, priming it for production of tasiARFs by SGS3, RDR6 and DCL4 action. This pathway is conserved across all land plants. By their repressing activity on Auxin Response Factors members, ARF2, ARF3 and ARF4, the tasiARFs control phase change and leaf patterning. Our lab and others have shown that TAS3 is also expressed in the root of Arabidopsis, raising the question of the role played by the TAS3 pathway in root development. We have shown that theTAS3 pathway, through the tasiARFs action, plays an essential role in the control of lateral rootgrowth. We have demonstrated that the TAS3 pathway acts downstream of auxin, to maintain the proper zonation and abundance of the Auxin Response Factors ARF2, ARF3 and ARF4. In addition,we unravelled a complex set of feedbacks of these ARFs on miR390 expression. Although the mechanisms of TAS3 processing have been identified through various genetic screens our knowledge of the subcellular organization of this pathway remains essentially unknown. For this reason we have chosen to study the subcellular localization of the TAS3 pathway, and focused on AGO7 which represents a specific element of this pathway. We have shown that AGO7, RDR6 and SGS3 accumulate in cytoplasmic foci, dubbed siRNA bodies. We have observed colocalization between these siRNA bodies and markers of the stress granules and a membrane-associated viral protein. Finally we have demonstrated the functional relevance of the cytoplasmic localization of AGO7 for the biogenesis of tasiARFs. Our work has led to a better understanding of the mechanisms underlying the action the TAS3 pathway during the development of Arabidopsis.

This book, a short and easy read with common sense coaching and easy to follow exercises, takes beginning acting students through techniques and improvisations that will help them overcome perhaps their greatest fear: making “The Big Choice.” Using sound examples from classical as well as contemporary plays, Herb Parker makes it easier for the theatrical neophyte by making the case that a play is “about human beings caught in an outrageous situation, caused by love.” “Actor, director, teacher Herb Parker has given us a splendid new book about acting. He analyzes this ancient art in clear, precise, engaging prose while dispensing acute, methodical and perceptive advice. Brilliant actor that he is, Herb understands the craft, the exceptional director gently leads the actor toward a fully realized performance and the thoughtful, caring teacher explains it all to the reader (student). This is the book of acting you need and one you will cherish.” / https://dc.etsu.edu/etsu_books/1052/thumbnail.jpg

Interaction between people can be seen as a distinctive feature of 'post-industrial society'. In this study I investigate some of the conditions for this encounter in private service work in Sweden. I start by discussing some important concepts: service, service encounter and emotional labour. Three parties in an interactional triangle can be perceived: the service enterprise, the service worker and the customer. The service encounter is embedded in organisational frames. Recruiting for social competence and training for selling interaction are two facets of these frames. In interactive service work, control is complicated by the fact that a third party, the customer, is involved and that the borders between worker, work process and result are somewhat indistinct. Indirect forms of control can be used to affect workers' attitudes and thinking, as well as behaviour. Service work can be described as a form of acting. Different service workers will identify differently with their work role. In my study I observe both positive and negative experiences of work. A majority report that they sometimes are so tired of people that they want to be alone after work. I distinguish two dimensions of interactive service work: type of interaction and sales situation. Interaction can be more or less important, and the sales situation can be more or less concealed. Based on these dimensions I suggest a typology to illustrate some differences between different service occupations. Four types are suggested: (1) Work first, and customer later; (2) Personalised services; (3) Routine selling; and (4) Persuasive selling. In the second group we find the experts of interaction, but also the strongest signs of social strain.

Human Resource personnel as part of their daily jobs provide a service to other employees within a mining industry. These service workers may experience dissonance between their actual feelings and the feelings they are expected to display. For these service workers to be more engaged at work, emotional intelligence and social support is vital. If these factors are not in place, their well-being may be in jeopardy. The objective of this research was to determine the relationship between Emotion Work, Emotional Intelligence, Well-being and Social Support of service workers in a human resource field within a mining industry. A cross-sectional survey design was used. The study population (n = 229) consisted of human resource personnel in the Limpopo and North West Province. The Greek Emotional Intelligence Scale (GEIS), Frankfurt Emotion Work Scales, Utrecht Work Engagement Scale, Oldenburg Burnout Inventory and Social Support Scale, as well as a biographical questionnaire, were used as measuring instruments. Cronbach alpha coefficients, factor analysis, inter-item correlation coefficients, Pearson product moment correlation coefficient and stepwise multiple regression analysis were used to analyse the data. An analysis of the data indicated that correlations between the following constructs are statistically and practically significant. The results show that Positive Display is statistically and positively practically significantly related (medium effect) to Interaction Control. Caring/Empathy is positively practically significantly related to Positive Display (medium effect). Furthermore the Control of Emotions (medium effect) and Emotion Management (large effect) are both positively practically significantly related to Caring/Empathy. Emotional Resilience however is negatively practically significantly related to Caring and Empathy (medium effect). Emotion Expression Recognition is positively practically significantly related to Control of Emotion (medium effect). However, both Exhaustion (medium effect) and Emotional Resilience (medium effect) are negatively practically significantly related to Control of Emotions. Engagement is positively practically significant (medium effect) to Emotion Management. Emotion Resilience (medium effect) positively correlates with Exhaustion while Engagement (medium effect) negatively correlates with Exhaustion. Engagement positively practically correlates with Resilience (medium effect). Social Support of both supervisor and co-workers positively relates to engagement to a medium effect. Principal component analysis performed on the GEIS resulted in a four-factor solution. The first factor was Caring and Empathy, which includes the willingness of an individual to help other people and understand others' feelings. The second factor was Control of Emotion, which is the ability of the individual to control and regulate emotions within themselves and others. Emotion Expression/Recognition, which is the ability of the individual to express and recognise his or her own emotional reactions, was the third factor, and the fourth was Emotion Management, which is the ability of an individual to process emotional information with regard to perception, assimilation, understanding and management of emotions. All four factors correlate with that of the GEIS originally developed by Tsaousis (2007) and accounted for 31% of the total variance in emotional intelligence. A Multiple Regression Analysis with Exhaustion as dependent variable was carried out. The results show that Emotion Work factors accounted for 2% of the total variance and Emotional Intelligence factors for 12% of the total variance. More specifically it seems that the lack of Caring and Empathy and Emotion Management predicted Exhaustion in this regard. However, when Emotional Intelligence factors were entered into the model, an increase of 10% variance was shown of the variance explained in Exhaustion. Emotion Work, Emotional Intelligence and Social Support predicted 14% of the variance explained in the level of Exhaustion by participants. A Multiple Regression analysis with Emotional Resilience as dependent variable was carried out. The results show that Emotion Work factors accounted for 6% of the total variance. More specifically; it seems that Dissonance predicted the level of Emotional Resilience. When Emotional Intelligence factors were entered into the model, an increase of 15% was shown. Caring and Empathy and Control of Emotions predicted Emotional Intelligence the best. Lastly, when Social Support factors were entered into the regression analysis, the variance explained showed an increase of 5%. Support of Family and Others predicted Emotional Resilience the best. In total, Emotion Work, Emotional Intelligence and Social Support factors explained 20% of the variance in Emotional Resilience. A Multiple Regression Analysis with Engagement as dependent variable with Emotion Work factors, Emotional Intelligence factors and Social Support as predictors of Engagement was done. Entry of Emotion Work factors at the first step of the regression analysis did not produce a statistically significant model and only accounted for 1% of the variance. However, when Emotional Intelligence factors were entered in the second step of the analysis, it accounted for approximately 7% of the variance. More specifically, it seems that Caring and Empathy predicted Engagement. When Social Support factors were entered into the third step of the analysis, an increase of 27% was found. All the Social Support factors (Social Support of Family and Others, Supervisors and Co-workers) accounted for 27% of the variance explained in Engagement. Emotion Work, Emotional Intelligence and Social Support predicted 33% of the total variance explained in the level of Engagement. Limitations within the study were identified, and recommendations were made for human resource personnel in a mining industry, as well as for future research. / Thesis (M.A. (Industrial Psychology))--North-West University, Potchefstroom Campus, 2009.

Human Resource personnel as part of their daily jobs provide a service to other employees within a mining industry. These service workers may experience dissonance between their actual feelings and the feelings they are expected to display. For these service workers to be more engaged at work, emotional intelligence and social support is vital. If these factors are not in place, their well-being may be in jeopardy. The objective of this research was to determine the relationship between Emotion Work, Emotional Intelligence, Well-being and Social Support of service workers in a human resource field within a mining industry. A cross-sectional survey design was used. The study population (n = 229) consisted of human resource personnel in the Limpopo and North West Province. The Greek Emotional Intelligence Scale (GEIS), Frankfurt Emotion Work Scales, Utrecht Work Engagement Scale, Oldenburg Burnout Inventory and Social Support Scale, as well as a biographical questionnaire, were used as measuring instruments. Cronbach alpha coefficients, factor analysis, inter-item correlation coefficients, Pearson product moment correlation coefficient and stepwise multiple regression analysis were used to analyse the data. An analysis of the data indicated that correlations between the following constructs are statistically and practically significant. The results show that Positive Display is statistically and positively practically significantly related (medium effect) to Interaction Control. Caring/Empathy is positively practically significantly related to Positive Display (medium effect). Furthermore the Control of Emotions (medium effect) and Emotion Management (large effect) are both positively practically significantly related to Caring/Empathy. Emotional Resilience however is negatively practically significantly related to Caring and Empathy (medium effect). Emotion Expression Recognition is positively practically significantly related to Control of Emotion (medium effect). However, both Exhaustion (medium effect) and Emotional Resilience (medium effect) are negatively practically significantly related to Control of Emotions. Engagement is positively practically significant (medium effect) to Emotion Management. Emotion Resilience (medium effect) positively correlates with Exhaustion while Engagement (medium effect) negatively correlates with Exhaustion. Engagement positively practically correlates with Resilience (medium effect). Social Support of both supervisor and co-workers positively relates to engagement to a medium effect. Principal component analysis performed on the GEIS resulted in a four-factor solution. The first factor was Caring and Empathy, which includes the willingness of an individual to help other people and understand others' feelings. The second factor was Control of Emotion, which is the ability of the individual to control and regulate emotions within themselves and others. Emotion Expression/Recognition, which is the ability of the individual to express and recognise his or her own emotional reactions, was the third factor, and the fourth was Emotion Management, which is the ability of an individual to process emotional information with regard to perception, assimilation, understanding and management of emotions. All four factors correlate with that of the GEIS originally developed by Tsaousis (2007) and accounted for 31% of the total variance in emotional intelligence. A Multiple Regression Analysis with Exhaustion as dependent variable was carried out. The results show that Emotion Work factors accounted for 2% of the total variance and Emotional Intelligence factors for 12% of the total variance. More specifically it seems that the lack of Caring and Empathy and Emotion Management predicted Exhaustion in this regard. However, when Emotional Intelligence factors were entered into the model, an increase of 10% variance was shown of the variance explained in Exhaustion. Emotion Work, Emotional Intelligence and Social Support predicted 14% of the variance explained in the level of Exhaustion by participants. A Multiple Regression analysis with Emotional Resilience as dependent variable was carried out. The results show that Emotion Work factors accounted for 6% of the total variance. More specifically; it seems that Dissonance predicted the level of Emotional Resilience. When Emotional Intelligence factors were entered into the model, an increase of 15% was shown. Caring and Empathy and Control of Emotions predicted Emotional Intelligence the best. Lastly, when Social Support factors were entered into the regression analysis, the variance explained showed an increase of 5%. Support of Family and Others predicted Emotional Resilience the best. In total, Emotion Work, Emotional Intelligence and Social Support factors explained 20% of the variance in Emotional Resilience. A Multiple Regression Analysis with Engagement as dependent variable with Emotion Work factors, Emotional Intelligence factors and Social Support as predictors of Engagement was done. Entry of Emotion Work factors at the first step of the regression analysis did not produce a statistically significant model and only accounted for 1% of the variance. However, when Emotional Intelligence factors were entered in the second step of the analysis, it accounted for approximately 7% of the variance. More specifically, it seems that Caring and Empathy predicted Engagement. When Social Support factors were entered into the third step of the analysis, an increase of 27% was found. All the Social Support factors (Social Support of Family and Others, Supervisors and Co-workers) accounted for 27% of the variance explained in Engagement. Emotion Work, Emotional Intelligence and Social Support predicted 33% of the total variance explained in the level of Engagement. Limitations within the study were identified, and recommendations were made for human resource personnel in a mining industry, as well as for future research. / Thesis (M.A. (Industrial Psychology))--North-West University, Potchefstroom Campus, 2009.

Abstract Ph.D. Thesis School of Education, The University of Queensland Ira Hal Seidenstein What is the nature of embodied practice, creativity and integral learning in Professional Learning for Acting (PLA) in Australia? An overall purpose of the research and thesis is to bring illumination to a lively heterodoxy in PLA. Heterodoxy is Pierre Bourdieu‘s definition of new or alternative influences in a field. The heterodoxy in PLA now has a rich body of techniques, principles, and knowledge to revitalize the field and environment of PLA. The research and thesis encounter the complexity of Professional Learning in Acting to understand the potential of new paradigms. To locate the context of the research, Bourdieu‘s Social Theory of Education related to his definition of culture and fields as dependent on doxa - and legitimacy - have provided the semiotics for the research. The body of the research and data collection was via ethnography and a primary practice of participant-observation. An inter-connected trilogy of topics was selected as priority issues in the field of PLA. The trilogy is: Embodied Practice, Creativity, and Integral Learning. The three areas have been often developed separately, initially stemming from theorist-practitioners Konstantin Stanislavsky and Jacques Copeau in the early twentieth-century. Since that time, biomechanical research and understanding in the scope of creativity to the mind-body continuum has blossomed in other fields. Yet the frameworks for dialogue with issues related to social theory in education are based on utilitarian, industrial, didactic, and Cartesian semiotics, so that little discussion integrates the complexity of, for example, the trilogy focus of the research. Marranca, founder and editor of Performing Arts Journal since 1976, has critiqued performing arts education where PLA is located. A central criticism she offers is this, ―At its core theatre study lacks sustained intellectual rigor and breadth‖ (Marranca, 1995, p. 1). This concern for the intellectual encounter may be engaged through Embodied Practices, Creativity, and Integral Learning. Some recent developments into complex encounters includes research into Vygotsky‘s value for adult education and creativity (John-Steiner & Moran, 2002), and research into consciousness paradigms for PLA reveal new potentialities into PLA (Meyer-Dinkgrafë, 2001; Yarrow 2002). Complexity science can be used to iii create such paradigms in PLA or in Education (Davis, 2004; Davis, Sumara, & Luce-Kapler, 2000; Morowitz, 2002; Wolfram, 2002) There is a body of research, techniques and knowledge that brings together East and West theatre and acting practices. This body of information is generally outside the perimeter of curriculum in Professional Learning for Acting. The knowledge has been gathered by the luminaries of acting, yet it is rarely allowed legitimacy in academia, in practice. The East-West practices may allow for greater embodied learning as they make use of integral body-mind practices. The research had quite physical, active levels of participation-observation components in the three Case Studies that dealt with a nationally accredited course in acting, an independent actor/artist in the process of creating a performance, and an established professional acting company. The participant-observation took place within ten theatre productions, extensive physical, and vocal training, performances, and touring.

Made available in DSpace on 2014-08-20T13:25:43Z (GMT). No. of bitstreams: 1 Tese_Clauber_Mateus_Priebe_Bervald.pdf: 1713348 bytes, checksum: c9ae92634bc72e4a44156ded5d351214 (MD5) Previous issue date: 2009-08-09 / The use of sequences of DNA has been the base of comparative genomics for evolutionary studies. The transfer of information from model species to agricultural species has been revolutionizing the molecular genetics and strategies of crop improvement. The combination of classic methods of genetics and breeding with molecular technologies of genomic analysis opens a new perspective for the increase of the understanding of the genetic basis and the acceleration of breeding programs. Bioinformatics is becoming a tool that will be an essential part of the plant scientific research contributing to this challenge, and allowing inferences of function, structure and evolution of genes and genomes, search of markers, primers design, among other possibilities. In this sense, three studies of comparative genomics in grasses were accomplished, using bioinformatics tools. In the first work, the objective was to analyze the microsatellite abundance in genome fractions of 13 species of the genus Oryza, describing the rates, frequencies and pattern of distribution of the different microsatellites. The microsatellite frequency varies inversely proportional to the size of the genome of the specie of the genus Oryza. The A genome species presented the highest occurrences and frequencies for all types and total microsatellites. The trimers composed by cytosines and guanines presented the percentile largest of occurrence among the species, without direct relationship with the GC content of the species. In the second work, the promoter region of 1,000 bases pairs of the genes OsNramp of Oryza sativa subsp japonica cv. Nipponbare was investigated as for the abundance of cis-acting elements. The sequences were analyzed using the software Signal Scan Search of the website Plant Cis-acting Regulatory DNA Elements (PLACE) to the identification of different cis-acting elements present in each one of the promoter regions. Were detected 170 different cis-acting elements in the upstream region of the genes members of the family OsNramp subsp japonica cv. Nipponbare. A total of 14 elements were common to the eight members of the family OsNramp subsp japonica cv. Nipponbare. The element CACTFTPPCA1 was the most frequent motif in the promoter region of the genes of the family OsNramp of Oryza sativa subsp japonica cv. Nipponbare. The objective of the last work was to compare the sequences of oats with the species Arabidopsis, barley, rice, sugarcane, Sorghum, wheat and corn, looking for to infer evolutionary relationships of those species with the oats. Sequences of eight species of plants evaluable at the present moment, in the Unigene data bank in the National Center for Biotechnology Information - NCBI were downloaded and comparisons with oat made using the BLASTn and tBLASTx tools. It was observed that the bank of ESTs of oats UNIGENE-NCBI consists from many sequences a little similar to other species compared to nucleotides (46,72%) and aminoacids (74,97%). Only 4,56% of the sequences present in the UNIGENE-NCBI oat EST bank presents high similarity with sequences of mono and dicotyledonous species. / O uso de seqüências de DNA tem sido à base da genômica comparativa para estudos evolucionários e a transferência de informações de espécies modelo para espécies agrícolas tem revolucionado a genética molecular e estratégias de melhoramento das culturas. A combinação de métodos clássicos de genética e melhoramento com tecnologias moleculares de análise genômica abre uma nova perspectiva para a ampliação do conhecimento das bases genéticas e aceleração de programas de melhoramento. A bioinformática está se tornando em uma ferramenta que será parte essencial na pesquisa científica de plantas e que poderá contribuir muito para este desafio, permitindo fazer inferências de função, estrutura e evolução de genes e genomas, busca de marcadores, desenho de primers, entre outras possibilidades. Neste sentido, três estudos de genômica comparativa em gramíneas foram realizados, utilizando a bioinformática como ferramenta. No primeiro trabalho, o objetivo do trabalho foi analisar a abundância de microssatélites no genoma de 13 espécies do gênero Oryza obtidas do GeneBank do National Center for Biotechnology Information NCBI, com programa SSRLocator, descrevendo as taxas, freqüências e padrão de distribuição dos diferentes microssatélites. A freqüência de microssatélites varia inversamente proporcional ao tamanho do genoma da espécie no gênero Oryza. As espécies de genoma A apresentam as maiores freqüências para todos os tipos de microssatélites e totais no gênero Oryza. Os trímeros compostos por citosinas e guaninas apresentam o maior percentual de ocorrência entre as espécies de gênero Oryza, sem relação direta com o conteúdo GC da espécie. No segundo trabalho, a região promotora de 1000 pares de bases dos genes OsNramp de Oryza sativa subsp japonica cv. Nipponbare foi investigada quanto à abundância de elementos cis-acting. As seqüências foram analisadas utilizando o programa Signal Scan Search do portal Plant Cis-acting Regulatory DNA Elements (PLACE) para a identificação dos diferentes elementos cis-acting presentes em cada uma das regiões promotoras. Foram detectados 170 diferentes elementos cis-acting na região promotora dos genes membros da família OsNramp, sendo um total de 14 elementos comuns a região promotora dos oito membros da família gênica OsNramp de Oryza sativa subsp. japonica. O elemento CACTFTPPCA1 foi o motivo mais freqüente na região promotora dos genes membros da família OsNramp de Oryza sativa subsp. japonica. O objetivo do último trabalho foi comparar as seqüências de ESTs de aveia com as espécies Arabidopsis, cevada, arroz, cana-de-açúcar, sorgo, trigo e milho, buscando inferir relações evolutivas dessas espécies com a aveia. Seqüências de oito espécies de plantas disponíveis atualmente, no banco de dados Unigene no National Center for Biotechnology Information - NCBI foram baixadas e as comparações com a aveia foram feitas utilizando as ferramentas de BLASTn e tBLASTx. Observou-se que o banco de ESTs de aveia UNIGENE-NCBI consiste de muitas seqüências pouco similares a outras espécies comparados a nucleotídeos (46,72%) e aminoácidos (74,97%). Uma minoria de 4,56% das seqüências presentes no banco de ESTs de aveia UNIGENE-NCBI apresentam alta similaridade com seqüências de espécies de mono e dicotiledôneas.

Orientador: Rubens Jose Souza Brito / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Artes / Made available in DSpace on 2018-08-07T11:26:28Z (GMT). No. of bitstreams: 1 Doria_RobertoRodriguez_M.pdf: 10788175 bytes, checksum: 584dfd87e2b95ad594052728b9c20c8d (MD5) Previous issue date: 2005 / Resumo: O texto que constitui este trabalho é uma reflexão teórica sobre processo criativo através da máscara desenvolvida com os atores da Companhia Teatral Nosconosco do Rio de Janeiro. Este trabalho parte de uma experiência prática na construção de um espetáculo teatral e seu desenvolvimento criativo com objetos de uso cotidiano que levam o ator ao estado da máscara e sua reflexão teórica / Abstract: The text of the present work is a theoretical reflection about the creative process through the mask, developed with actors from the Companhia Teatral Nosconosco, from Rio de Janeiro. This work starts from a practical experience in a play making of using daily ordinary objects that take the actor into the mask state and its theoretical reflection / Mestrado / Mestre em Artes

ABSTRACT Agnes of God is the story of a nun (Agnes) who gets pregnant while living in a convent. She almost dies after giving birth to a baby, who was later found dead in a garbage can. The cast consists of three members: Agnes; Mother Miriam Ruth (Mother Superior); and Dr. Martha Livingstone, the court appointed psychiatrist. Faith is tested as Dr. Livingstone draws closer to uncovering the truth surrounding the conception, birth, and death of Agnes’ baby. I performed the role of Mother Superior in the UNO production of Agnes of God. This thesis documents my rehearsal and performance journey. It includes a rehearsal journal and an evaluation of my performance. The play was performed in the Lab Theatre of the Performing Arts Center on November 5 and 6; November 14 and 15; and on November 19 and 20. Agnes of God is based on a true story. Keywords: Mother Superior, Agnes, Pielmeier, Sacrifice, Nun, Maureen Murphy

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