This thesis concerns Swedish translations of the Dutch independently used modal auxiliary verbs moeten, kunnen and willen in six Dutch novels from the period 1973-2000. Previous research has shown that independent use occurs frequently in Dutch but less frequently in Swedish. But there is still a need for large-scale comparative research.  For this analysis, I have conducted a corpus study based on systematically repeated searches for the three verbs. The goal of the study was to examine how these independently used modal auxiliary verbs were translated into Swedish and what patterns could be seen through this comparative study. Through this comparative study, I wanted to better highlight the differences and similarities between the languages regarding independently used modal auxiliary verbs.  The results showed that independently used modal auxiliary verbs occur more frequently in the corpus in Dutch than in Swedish and that the Dutch independently used modal auxiliary verbs were translated with a corresponding independently used modal in Swedish in about a fourth of the cases. But the results also show similarities in this area. / Deze scriptie betreft Zweedse vertalingen van de Nederlandse zelfstandig gebruikte modale hulpwerkwoorden moeten, kunnen en willen in zes Nederlandstalige romans uit de periode 1973-2000. Eerder onderzoek heeft vastgesteld dat zelfstandig gebruik vlot toegelaten is in het Nederlands maar minder frequent voorkomt in het Zweeds. Toch ontbreekt er grootschalig contrastief onderzoek.  Voor deze analyse werd een corpusonderzoek verricht op basis van systematisch herhaalde zoekopdrachten voor de drie modalen. Het doel van het onderzoek was om na te gaan hoe deze zelfstandig gebruikte modale hulpwerkwoorden in het Zweeds vertaald werden en wat voor patronen men kon zien door dit contrastief onderzoek. Met dit contrastief onderzoek wilde ik de verschillen en gelijkenissen tussen de talen wat betreft zelfstandig gebruikte modale hulpwerkwoorden beter belichten.  De resultaten lieten zien dat zelfstandig gebruikte modale werkwoorden in het corpus vaker voorkomen in het Nederlands dan in het Zweeds en dat de Nederlandse zelfstandig gebruikte modale hulpwerkwoorden in ongeveer een vierde van de gevallen met een corresponderende zelfstandig gebruikte modaal in het Zweeds vertaald werden. Maar de resultaten vertonen ook dat er toch gelijkenissen zijn op dit gebied.

The development ofNursing and Nursing Education in Venda is intimately related to the work of the early missionaries, Western education and medical developments. The growth of nursing and health services had a reciprocal relationship. One was not possible without the other, viz the history of missionary education, hospitals, health services, sociocultural and political development. Each stage ofthese developments was an important step in laying the foundation of modem professional nursing and nursing education. Before contact with Western medicine, health and midwifery services were provided by traditional healers, nurses and midwives. Divination was used to: diagnose disease, identify the witch, determine medicines to cure the disease. Western nursing developed through stages of: lay nursing, hospital certificated, auxiliary nurses and midwives, registered nurses and midwives, comprehensive general nurse and midwife. The history of Nursing and Nursing Education is thus part and parcel of the social developments of the country it serves. / Health Studies / M.A. (Nursing Science)

The purpose of this study was to determine the contribution of nursing auxiliaries towards health care services against their scope of practice. Nursing auxiliaries practising within the Elim, Letaba, Malamulele, Nkhensani, Shiluvana and Tintswalo hospitals in the Gazankulu Health Administration, in the Northern Transvaal Province, constituted the target population. The findings revealed that nursing auxiliaries are not functioning strictly according to their scope of practice. They are an essential component of nursing services in Gazankulu by rendering a major contribution towards health care services in fulfilling their scope of practice-role. Apart from their prescribed practice-role, they are also engaged in activities that should be performed by enrolled and professional nurses as well as doctors and general assistants. There appears to be a need for education for all categories of nursing staff regarding the scope of practice of nursing auxiliaries for improving the effective utilisation of this category of nursing personnel / M.A. (Nursing Science) / Health Studies

隨著全球化趨勢以及企業間越加激烈之競爭關係，生產者為滿足消費者之即時需求，即常透過多式運送模式將商品跨國快速遞送。物流服務之涵蓋範圍因而越發廣泛並持續擴展，除了實體之商品配送服務，並完善整合不同運送模式，且將高效率之管理、關務與其他商業活動納入範疇。此種整合型物流服務之出現，除了免除生產者與貿易商物流管理之額外負擔，以利其專注本業外，高品質之物流服務尚可提昇運輸服務業之生產力，同時並增進全球經濟福利之發展。 鑑於物流服務以及供應鏈管理服務均非屬W/120中之獨立部門，WTO會員即以CPC為基礎發展出物流服務清單與相關提案，以期提昇物流服務在市場開放與國民待遇之承諾情形。 本文首先介紹物流服務之貿易實務，概述物流服務之貿易概況、產業趨勢與貿易障礙；其次探討GATS 架構下物流服務之相關規範與會員提案，並分別就相關之GATS條文與會員提案發展為剖析。此外，本文並分析WTO會員就物流服務之現行承諾狀況與新回合之開放情形。最後，本文以物流服務之分類議題、定義議題與相關服務之新回合談判進展為重點，進行深入之分析並嘗試提出可行之解決之道。 / In the wake of globalization and increasingly fierce competition, goods often need to be transported through several countries (by road, rail, air or water) as quickly as possible in order to respond "just in time" to customer demand. Logistics services comprise a wide range of services and are constantly evolving In addition to the physical transportation of goods, based on an optimized combination of available modes of transport, it involves the efficient handling of management, customs and other business matters. The existence of integrated logistics services relieve manufacturers and traders of the burden of logistics management and allow them to focus on their core competencies. High-quality logistics services could also increase the productivity of the transport sector, and improve global economic welfare at the same time. Since neither logistics nor supply chain management is identified as a distinct industry on the W/120, WTO members that rely on the CPC have advocated developing a freight logistics checklist and related proposals in the hope of improving meaningful market access and national treatment commitments for logistics services. The thesis first introduces the practices of trade in logistics services, including the profiles, trends of industrial development, and trade barriers on trade in logistics services. Then the thesis discusses the related regulations and proposals of logistics services under the GATS framework, explaining the related GATS articles for logistics servies and clarifying their influence as well. Besides, the thesis also exam members’ existing commitments and offers on the logistics services. Finally, the thesis focuses on the classification, definition and negotiation issues on the logistics services, analyzing existing difficulties and trying to submit possible solutions.

Approved for public release, distribution is unlimited / Commander, Logistics Group, Western Pacific (COMLOGWESTPAC) is concerned with the delivery of high priority material, ordnance, and passengers to U.S. Navy ships due to a very large operations area and limited Combat Logistics Force (CLF) assets. High-speed vessels (HSVs) may have the potential to improve the delivery of these materials when used to complement existing logistics shuttle ships. This thesis quantifies current levels of traditional naval logistics support and provides comparison to HSV-based alternatives in various scenarios. The CLF Scenario Analysis Tool (CLFSAT), a newly developed discrete event simulation model of naval logistics support, performs the analysis. Given a scenario depicting combatant movements and operations, CLFSAT provides insight into the comparative performance of different supporting naval logistics force structures. This analysis determines that HSVs can be effective logistics platforms in specific scenarios when distributing high priority material, ordnance, and stores. HSVs are very effective in small theaters with short transit distances, but for larger theaters, their effectiveness is inversely proportional to distance from the Forward Logistics Site. Regardless of theater size, HSVs show significant improvements in theater distribution of "low density, high priority" cargo, such as precision guided munitions (PGMs) or critical repair parts when customers are outside COD range. / Lieutenant Commander, United States Navy

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal.

Strikt anaerobe Bakterien wie Clostridium difficile und C. scatologenes verwenden GRE, um die chemisch ungünstige Decarboxylierung von 4-Hydroxyphenylacetat zu p-Cresol zu katalysieren. Das Enzymsystem besteht aus einer Decarboxylase und dem zugehörigen Aktivierungsenzym. Die 4-Hydroxyphenylacetat-Decarboxylase (4Hpad) besitzt zusätzlich zum Protein-basierten Glycinradikal eine weitere Untereinheit mit bis zu zwei [4Fe-4S] Clustern und repräsentiert hierdurch eine neue Klasse von Fe/S-Cluster-haltigen GREs, die aromatische Verbindungen umsetzen. Das Aktivierungsenzym (4Hpad-AE) weicht vom Standardtypus ab, indem es zusätzlich zum S-Adenosylmethionin(SAM)-bindenden [4Fe-4S]-Cluster (RS-Cluster) mindestens einen weiteren [4Fe-4S]-Cluster bindet. In dieser Studie wurden heterologe Expressions- und Reinigungsprotokolle für 4Hpad und 4Hpad-AE entwickelt. Kristallstrukturen von 4Hpad cokristallisiert mit den Substraten (4-Hydroxyphenylacetat, 3,4-Dihydroxyphenylacetat) und dem Inhibitor (4-Hydroxyphenylacetamid) zeigten geringe strukturelle Änderungen im aktiven Zentrum des Proteins. Die Radikalbildung am 4Hpad-AE wurde durch die Überprüfung einer klassischen reduktiven Spaltung von SAM zu den Reaktionsprodukten 5’-Deoxyadenosin und Methionin bestätigt. EPR- und Mössbauer-Spektroskopische Analysen zeigten, dass 4Hpad-AE mindestens einen zusätzlichen [4Fe-4S] Cluster neben dem einzelnen RS-Cluster enthält. Die katalytische Notwendigkeit eines zusätzlichen Clusters wurde durch eine Mutationsanalyse untersucht, wobei eine verkürzte Version des Enzyms ohne die zusätzliche Cystein-reiche Insertion konstruiert wurde. Das verkürzte Mutante ohne die Bindungsmotive für die zusätzlichen Cluster gekennzeichnet, die Konfiguration, Stöchiometrie und die Funktion der zusätzlichen Cluster diagnostizieren. / 4-hydroxyphenylacetate decarboxylase (4Hpad) is a two [4Fe-4S] cluster containing glycyl radical enzyme proposed to use a glycyl/thiyl radical dyad to catalyze the last step of tyrosine fermentation in Clostridium difficile and C. scatologenes by a Kolbe-type decarboxylation. The decarboxylation product p-cresol is a virulence factor of the human pathogen C. difficile. The small subunit of 4Hpad may have a regulatory function with the Fe/S clusters involved in complex formation and radical dissipation in the absence of substrate. The respective activating enzyme (4Hpad-AE) has one or two [4Fe-4S] cluster(s) in addition to the SAM-binding [4Fe-4S] cluster (RS cluster). The role of these auxiliary clusters is still under debate with proposed functions including structural integrity and conduit for electron transfer to the RS cluster. This study shows the optimized expression and purification protocols for the decarboxylase and the co-crystallization experiments and binding studies with 4-hydroxy-phenylacetate and 3,4-dihydroxyphenylacetate and with the inhibitor 4-hydroxy-phenylacetamide. The purification and characterization of active site mutants of decarboxylase are also done. Concerning 4-HPAD-AE, we report on the purification of code-optimized variants, and on spectroscopic and kinetic studies to characterize the respective i) SAM binding enthalpies, ii) rates for reductive cleavage of SAM and iii) putative functions of the additional Fe/S clusters. The truncated mutant lacking the binding motifs for the auxiliary clusters is characterized to diagnose the configuration, stoichiometry and function of the auxiliary clusters.

Chemoselektive Peptidfragmentverknüpfungsmethoden sind ein zentrales Element der chemischen Peptid- und Proteinsynthese. Auf der Suche nach einem „universellen Werkzeug für die Peptidligation“ wurde in den vergangenen zwei Jahrzenten eine Vielzahl unterschiedlicher Hilfsmolekülen (Auxiliare) entwickelt. Trotz des enormen Forschungaufwandes blieben bisher verfügbare Nα-Auxiliare jedoch in ihrer Anwendung auf glycinhaltige Ligationsstellen beschränkt. Im Fall der häufig verwendeten säurelabilen Nα-Auxiliare müssen zudem starke Säuren oder Supersäuren eingesetzt werden, um das Hilfsmolekül nach der Peptidligation zu entfernen. Dabei wurde häufig die Spaltung der zuvor aufgebauten Amidbindung als unerwünschte Nebenreaktion beobachtet. In der vorliegenden Arbeit wurde die Synthese von acht Nα-Auxiliaren erarbeitet und deren Anwendung in der erweiterten nativen chemischen Ligation untersucht. Dabei konnten sechs Hilfsmoleküle identifiziert werden, welche die Auxiliar-Abspaltung unter mild-basischen Bedingungen ermöglichten. Von besonderer Bedeutung war das 2-Mercapto-2-phenethyl-Grundgerüst, welches im Gegensatz zu bisher beschriebenen Nα-Auxiliaren erstmals den Zugang zu sterisch anspruchsvollen Verknüpfungstellen, jenseits von Glycin, gestattete. Der Nutzen des Auxiliars wurde in die chemische Totalsynthese zweier antimikrobieller Peptide demonstriert. Durch die Verwendung eines 13C-markierten Hilfsmoleküls konnte der Mechanismus der radikalischen Auxiliar-Abspaltungsreaktion in NMR-Experimenten detailliert untersucht werden. Zusätzlich wurde das 2-Mercapto-2-phenethyl-Auxiliar in einer neuen Methode der chemischen Proteinsynthese eingesetzt, welche die gewünschten Proteine ohne eine einzige HPLC-Reinigung in reiner Form lieferte. Im letzen Teil der Arbeit wurde mit dem 2-Selenol-2-phenethyl-Grundgerüst erstmals die Anwendung eines Selenol-funktionalisierten Nα-Auxiliars beschrieben. Das Hilfsmolekül ermöglichte besonders schnelle Verknüpfungsreaktionen und konnte zudem rasch mit hoher Selektivität entfernt werden. / Chemoselective ligation methods are an essential element of chemical peptide and protein synthesis. The search of a „universal tool for peptide ligation“ led to a range of different ligation auxiliaries over the last two decades. Despite the intense research in this field established Nα-auxiliaries remained limited to glycine-containing ligation sites. Furthermore, the application of frequently used acid-labile Nα-auxiliaries requires strong acids or superacids to remove the auxiliary after the ligation reaction. Under these harsh acidic conditions the cleavage of the established amide bond has been observed as an undesired side-reaction. This work describes the synthesis of eight Nα-auxiliaries and their application in extended native chemical ligation. Six helping molecules were identified enabling auxiliary cleavage under mild-basic conditions. Perhaps most important and in contrast to previously reported Nα-auxiliaries, the 2-mercapto-2-phenethyl group facilitated access to sterically demanding ligation sites, beyond glycine. The synthetic utility of the auxiliary was demonstrated by the chemical total synthesis of two antimicrobial peptides. The application of a 13C-labelled scaffold allowed a detailled study oft the radical auxiliary cleavage reaction by NMR-spectroscopy. Additionally, the 2-mercapto-2-phenethyl auxiliary was utilised for a novel method of chemical protein synthesis, which delivered the desired proteins without a single HPLC-purification in high purity. Finally, a selenol-functionalized Nα-auxiliary is described for the first time by introducing the 2-selenol-2-phenethyl mojety. This scaffold enabled execptionally rapid peptide ligations and is readily removed with high selectivity.

No description available.

No description available.