O objetivo deste estudo foi investigar os efeitos do condicionamento radicular em diferentes tempos de aplicação na descontaminação das superfícies radiculares in vitro e avaliar seus efeitos no recobrimento radicular em seres humanos. Para o estudo in vitro, 132 fragmentos radiculares foram divididos em 11 grupos (n=12), de acordo com o tratamento: AF180 Raspagem e Alisamento Radicular (RAR) + aplicação de ácido fosfórico (AF) por 180 segundos; AF90 RAR + AF por 90 segundos; EDTA180 RAR + EDTA por 180 segundos; EDTA90 RAR + EDTA por 90 segundos; ACT180 RAR + ácido cítrico associado à tetraciclina (AC+T) por 180 segundos; ACT90 RAR + [AC+T] por 90 segundos; AC180 RAR + ácido cítrico (AC) por 180 segundos; AC90 RAR + AC por 90 segundos; T180 RAR + Tetraciclina ácida (T) por 180 segundos; T90 RAR + T por 90 segundos; Controle RAR. Os fragmentos foram analisados por meio de microscopia eletrônica de varredura para determinar o grau de descontaminação da superfície radicular, de acordo com os índices de rugosidade superficial (IRS), cálculo residual (ICR), perda de substância dentária (IPSD), presença de restos teciduais (IPRT) e remoção da smear layer (IRSL). Os resultados obtidos foram analisados estatisticamente pelo método de Kruskal-Wallis. No estudo clínico randomizado, 27 pacientes com idade variável entre 23 e 60 anos (39,9 ± 13,22 anos), de ambos os sexos, apresentando recessão gengival classes I ou II de Miller, foram aleatoriamente alocados em três diferentes grupos para avaliar os efeitos do condicionamento ácido no recobrimento radicular: G180- aplicação de ácido cítrico associado à tetraciclina (ACT) durante 3 minutos após RAR (n= 20 sítios); G90- aplicação de ACT durante 90 segundos após RAR (n= 20 sítios); G0- RAR (n= 20 sítios). Todos os sítios foram tratados por meio de enxerto de tecido conjuntivo subepitelial. As medidas de profundidade de sondagem (PS), nível de inserção clínica (NIC), altura da recessão (AR), quantidade de gengiva ceratinizada (GC), índice de sangramento à sondagem (ISS) e índice de placa (IPl) foram investigadas nos exames inicial e pós-operatórios de 1, 3, 6 e 12 meses. Os pacientes responderam a questionário para determinar a intensidade da sensibilidade radicular segundo escala de dor variável de 0 (ausente) a 10 (máximo de dor) no exame inicial, aos 7 e 14 dias, 1, 3, 6 e 12 meses pós-operatórios. O grupo controle apresentou os maiores índices de rugosidade superficial (2,0 ± 1,02) e remoção da smear layer (3,83 ± 1,19), enquanto que o grupo EDTA180 apresentou os menores índices de cálculo residual (1,33 ± 0,63), IPSD (1,0 ± 0,0), IPRT (1,08 ± 0,28) e IRSL (1,08 ± 0,28). Não houve diferença estatisticamente significante (p>0.05) entre os grupos EDTA180, EDTA 90, AC90, AC180, ACT90 e ACT180 em todos os índices investigados. No estudo clínico, observou-se ganho significante (p<0.0001) de inserção clínica, redução da altura da recessão e aumento da faixa de gengiva ceratinizada em todos os grupos, sem alteração significante da profundidade de sondagem (p> 0.05). O percentual médio de recobrimento radicular foi maior no G180 (59,58% ± 34,76%), porém sem diferenças significantes (p= 0.31) em relação aos grupos G90 (52,12% ± 30,98%) e G0 (40,87% ± 36,96%). Houve maior redução média da recessão no G90 (1,95 ± 1,27) do que no grupo controle (0,95 ± 1,05), porém sem diferenças significantes (p> 0.05) em relação ao G180 (1,20 ± 0,69). Observou-se maior quantidade de GC nos grupos G90 (3,50 ± 1,90) e G180 (4,05 ± 0,44) após 12 meses, com diferenças estatisticamente significantes (p=0.0018) em relação ao G0 (2,0 ± 1,41). Houve redução significante da sensibilidade radicular em todos os grupos (p< 0.0001), sem diferenças entre os grupos (p> 0.05). Esses achados sugerem que o uso de condicionamento da superfície radicular favorece o aumento da faixa de gengiva ceratinizada e a redução da recessão, com diminuição da sensibilidade radicular. Dentre os diferentes tipos de agentes condicionantes investigados, os melhores resultados foram observados com EDTA a 24%, AC pH 1,0 e ACT, podendo ser empregados pelos períodos de 90 segundos ou 3 minutos. / The aim of this study was to investigate the effects of root conditioning in different periods of time in decontamination of root surfaces in vitro and to evaluate the effects in root coverage in humans. For the in vitro study, 132 root fragments were separated in 11 groups, according to treatment: PA180 scaling and root planing (SRP) + phosphoric acid (PA) application for 180 seconds; PA90 SRP + PA application for 90 seconds; EDTA180 SRP + EDTA application for 180 seconds; EDTA90 SRP + EDTA application for 90 seconds; ACT180 SRP + citric acid associated to tetracycline (ACT) for 180 seconds; ACT90 SRP + ACT for 90 seconds; AC180 SRP + citric acid (CA) for 180 seconds; AC90 SRP + CA for 90 seconds; T180 SRP + acid tetracycline (T) for 180 seconds, T90 SRP + T for 90 seconds; Control SRP. Fragments were analysed by scanning electron microscopy to determine the degree of root surface decontamination, according to surface rugosity index (SRI), residual calculus index (RCI), loss of tooth substance index (LTSI), presence of tissue remnants index (PTRI) and removal of smear layer index (RSLI). The results obtained were statistically analysed according to Kruskal Wallis method. At the randomized clinical trial, 27 patients aged 23-60 years (39,9 ± 13,22 years), both genders, presenting Millers class I or II recessions, were randomly allocated into one of the following groups to investigate the effects of acid conditioning in root coverage: G180- application of citric acid associated to tetracycline (ACT) for 3 minutes after SRP(n= 20 sites); G90- application of ACT for 90 seconds after SRP (n= 20 sites); G0- RAR (n= 20 sites).All sites were treated by subepithelial connective tissue graft. Probing depth (PD), clinical attachment level (CAL), recession heigth (RH), width of keratinized gingiva (KGW), sulcular bleeding index and plaque index were investigated at baseline and at 1, 3, 6 and 12 months postoperative. Patients have answered a questionnaire to determine the intensity of hypersensitivity according to a pain scale varying from 0 (absent) to 10 (highest pain) at baseline and 7 and 14 days, 1, 3, 6 and 12 months postoperative. Control group showed the highest RSI (2.0 ± 1.02) and RSLI (3.83 ± 1.19), while EDTA180 showed the lowest RCI (1.33 ± 0.63), LTSI (1.0 ± 0.0), PTRI (1.08 ± 0.28) and RSLI (1.08 ± 0.28). No significant differences (p> 0.05) were observed among EDTA180, EDTA90, AC90, AC180, ACT90 and ACT180 at all investigated indexes. At randomized clinical trial, a significant (p< 0.0001) gain of attachment level, reduction of recession height and increase in keratinized gingiva width in all groups, along with no significant alteration in PD (p> 0.05).The mean percentage of root coverage was greater at G180 (59.58% ± 34.76%), but with no significant differences (p= 0.31) when compared to G90 (52.12% ± 30.98%) and G0 (40.87% ± 36.96%). A higher mean reduction of recession height was observed at G90 (1.95 ± 1.27) than at control group (0.95 ± 1.05), but with no significant differences (p> 0.05) when compared to G180 (1.20 ± 0.69). A wider keratinized gingiva was observed at G90 (3.50 ± 1.90) and G180 (4.05 ± 0.44) after 12 months, with significant differences (p=0.0018) when compared to G0 (2.0 ± 1.41). A significant reduction of hypersensitivity was observed at all groups (p< 0.0001), with no differences among groups (p> 0.05). These findings suggest that root conditioning favors an increase in keratinized gingiva width and reduction of recession height, along with reduction of hypersensitivity. Among the different types of conditioning agents, the best results were observed with 24% EDTA, CA pH 1.0 and ACT applied for 90 seconds or 3 minutes.

Orientador: Konstantin Georgiev Kostov / Resumo: Nos últimos anos, tem intensificado o emprego de plasmas em pressão atmosférica para diferentes aplicações. Com o desenvolvimento dos jatos de plasma em pressão atmosférica, alguns tratamentos precisos, como no campo biomédico ou em específicos processamentos de superfícies, tornaram-se mais frequentes. No entanto, a aplicação de plasma à objetos irregulares, dentro de tubos ou mesmo dentro de órgãos ocos é limitada quando se utilizam configurações convencionais de jatos de plasma. Portanto, essas limitações podem ser superadas com o desenvolvimento de jatos de plasma alongados ou gerados remotamente. Neste trabalho, duas configurações de jato de plasma longo visando diferentes campos de aplicação foram aperfeiçoadas e caracterizadas. Inicialmente foi desenvolvido um jato de plasma endoscópico (plasma endoscope) operando em configuração de descarga por barreira dielétrica (DBD) com dimensões milimétricas, versátil ao acoplamento em endoscópios típicos. Este jato de plasma pode operar com hélio ou neônio e conta com um canal externo e concêntrico de gás que permite a introdução de uma cortina de gás eletronegativo ao redor da pluma de plasma. A cortina de proteção a gás preserva a forma do jato de plasma quando operado dentro de cavidades fechadas. As dificuldades advindas do desenvolvimento deste foram investigadas quando diferentes gases foram testados como cortina de proteção dele, dentre estes, o dióxido de carbono se mostrou uma boa opção evitando a formação de descargas ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The use of atmospheric pressure plasmas for different purposes has increased in recent years. With the development of atmospheric pressure plasma jets, some precise treatments such as in the biomedical field or specific surface processing became more often. However, the delivery of plasma to irregular shaped objects, inside tubes or even hollow organs is limited with the use of conventional plasma jet configurations. Therefore, those limitations can be surpassed with the development of elongated or remotely generated plasma jets. In this work, two extended plasma jet configurations aiming at different application fields were further developed and characterized. Firstly, an endoscopic plasma jet (plasma endoscope) operating with a dielectric barrier discharge (DBD) configuration in millimeter dimensions that can be coupled to a typical endoscope was developed. This plasma jet can operate with helium or neon and counts with an external concentric shielding gas channel that provides the introduction of an electronegative gas curtain around the plasma plume. The shielding gas allows the preservation of the plasma jet shape when operated inside closed cavities. The construction difficulties arisen from the use of different feed and shielding gases were explored. Carbon dioxide was proven to be a good option for the curtain gas around the plasma plume avoiding the formation of parasitic discharges inside the shielding gas tube and the endoscopic housing. When operated with neon, th... (Complete abstract click electronic access below) / Doutor

Des études dans le domaine plasma-biologie ont démontré l'effet biocide des jets plasma à pression atmosphérique (JPPAs), faisant de ces dispositifs une alternative aux traitements classiques de décontamination microbiologique. Le gaz (ou mélange de gaz) utilisé et la puissance injectée dans le plasma ont été identifiés comme des paramètres importants influençant l'efficacité biocide des jets plasmas. Récemment, il a été rapporté qu'un autre facteur pouvait influencer l'effet biocide des jets plasmas : la nature de la cible en interaction avec le jet. Cette thèse propose d'étudier l'influence de la nature d'une cible sur les propriétés physiques et biocides des jets plasmas. Dans un premier temps, le jet plasma a été caractérisé en l'absence de cible à l'aide d'outils de diagnostics électriques (sonde de tension, sonde de courant, oscilloscope) et optiques (caméra ICCD, spectromètre optique). Ainsi, la caméra ICCD a permis l'observation de la propagation d'une onde d'ionisation à des vitesses très élevées (de l'ordre du km.s-1). Dans un second temps, la caractérisation a porté sur l'interaction du jet plasma avec d'une part une cible diélectrique (10-10 S.m-1) et d'autre part une cible conductrice (6.0 107 S.m-1). Les résultats montrent que lorsque l'onde d'ionisation atteint la cible diélectrique, celle-ci se propage de manière radiale sur la surface de la cible formant une onde d'ionisation surfacique. Pour la cible conductrice, un canal s'établit entre la source plasma et la surface de la cible, suivi éventuellement par la propagation d'une onde d'ionisation secondaire de la surface de la cible en direction de la sortie de la source plasma. Enfin, afin d'évaluer l'influence de la nature de la cible sur l'efficacité biocide du jet plasma, des suspensions d'endospores de Bacillus atrophaeus et de bactéries Escherichia Coli ont été utilisées comme indicateur biologique. Les endospores et les bactéries ont été inoculées sur la surface des cibles diélectriques et conductrices, puis exposées au jet plasma. Les résultats montrent des modifications différentes sur le manteau des spores et une efficacité biocide plus élevée pour la cible conductrice comparée à la cible diélectrique. En résumé, cette thèse montre que les effets biocides des jets plasmas doivent être évalués dans les conditions expérimentales qui correspondent à l'application envisagée. / Studies in the plasma-biology field have demonstrated the biocidal effect of atmospheric pressure plasma jets (JPPAs), making these devices an alternative to conventional biological decontamination treatments. The gas (or gas mixture) used and the power injected into the plasma were identified as the parameters influencing the biocidal efficacy of plasma jets. Recently, it has been reported that another factor may influence the biocidal effect of plasma jets: the nature of the target in interaction with the jet. This thesis proposes to study the influence of the nature of a target on the biocidal properties of plasma jets. Initially, the plasma jet was characterized in the absence of a target using electrical (voltage probe, current probe, oscilloscope) and optical (ICCD camera, optical spectrometer) diagnostic tools. The ICCD camera allowed the observation of the propagation of an ionization wave at very high velocities (of the order of km.s-1). Secondly, the characterization focused on the interaction of the plasma jet with a dielectric target (10-10 S.m-1) and a conductive target (6.0 107 S.m-1). The results show that when the ionization wave reaches the dielectric target, it propagates radially on the surface of the target forming a surface ionization wave. For the conductive target, a channel is established between the plasma source and the target surface, possibly followed by propagation of a secondary ionization wave from the target surface towards the plasma source outlet. Finally, the influence of the target nature on the biocidal efficacy of the plasma jet was studied. Endospores suspensions of Bacillus atrophaeus and Escherichia coli bacteria were used as biological indicator. Endospores and bacteria were inoculated on the surface of the dielectric and conductive target and exposed to the plasma jet. The results show different changes on the spores coat's and higher biocidal efficiency for the conductive target compared to the dielectric target. In summary, this thesis shows that the biocide effects of plasma jets should be evaluated under the experimental conditions corresponding to the intended applications.

Since the undertaking of urban planning as a prescriptive discipline, landscape projects have demonstrated their ability to integrate valuable cultural spaces with the construction of complex infrastructural systems, including systems that manage urban waste outflows. By the twenty-first century, urban planners have been tasked with the reclamation of derelict post-industrial sites and their abandoned infrastructural networks. The reclamation of these sites typically deploys complex operations in order to salvage and recycle valuable materials. These operations are also tasked with the disposal, stabilization or treatment of hazardous waste, contaminated soils and waterborne pollutants. Urban practitioners and theorists increasingly recognize the suitability of landscape as an interdisciplinary medium to expedite the reurbanization of these sites, assembling expertise from multiple engineering disciplines, horticultural and zoological science, and architectural design. The thesis proposition is a masterplan for the post-industrial Lachine Canal in Montréal, Québec. The masterplan integrates government plans to rehabilitate aging highway infrastructure through the adjacent, now defunct Turcot Rail Yard. Using the analytical mappings defined by Alan Berger in Drosscsape and Pierre Belanger’s “infrastructural landscapes” as a point of departure, the masterplan outlines a strategy to coordinate emergent waste diversion industries along the canal. Proposed interventions include a hybridized infrastructural landscape upon the abandoned rail yard to manage municipal organic waste, the effluents of brownfield reclamation, and construction debris. The application of phytoremediation landfarming and constructed wetlands comprise new landscapes that facilitate decontamination of existing brownfields along the Canal, promoting their reintegration with the surrounding urban environment. The thesis illustrates a speculative evolution of the site as an adaptively managed landscape, valued for its diverse biological wildlife habitat and for its recreational use by the citizens of Montréal.

Since the undertaking of urban planning as a prescriptive discipline, landscape projects have demonstrated their ability to integrate valuable cultural spaces with the construction of complex infrastructural systems, including systems that manage urban waste outflows. By the twenty-first century, urban planners have been tasked with the reclamation of derelict post-industrial sites and their abandoned infrastructural networks. The reclamation of these sites typically deploys complex operations in order to salvage and recycle valuable materials. These operations are also tasked with the disposal, stabilization or treatment of hazardous waste, contaminated soils and waterborne pollutants. Urban practitioners and theorists increasingly recognize the suitability of landscape as an interdisciplinary medium to expedite the reurbanization of these sites, assembling expertise from multiple engineering disciplines, horticultural and zoological science, and architectural design. The thesis proposition is a masterplan for the post-industrial Lachine Canal in Montréal, Québec. The masterplan integrates government plans to rehabilitate aging highway infrastructure through the adjacent, now defunct Turcot Rail Yard. Using the analytical mappings defined by Alan Berger in Drosscsape and Pierre Belanger’s “infrastructural landscapes” as a point of departure, the masterplan outlines a strategy to coordinate emergent waste diversion industries along the canal. Proposed interventions include a hybridized infrastructural landscape upon the abandoned rail yard to manage municipal organic waste, the effluents of brownfield reclamation, and construction debris. The application of phytoremediation landfarming and constructed wetlands comprise new landscapes that facilitate decontamination of existing brownfields along the Canal, promoting their reintegration with the surrounding urban environment. The thesis illustrates a speculative evolution of the site as an adaptively managed landscape, valued for its diverse biological wildlife habitat and for its recreational use by the citizens of Montréal.

Good soil condition belongs to the basic requirements of well-ballanced environment. That is why the soil has to correspond to hygienic requirements and be protected against substances harmful to human health, e.g. toxic materials, germs of transmissible illnesses and parasites. The most common cause of soil contamination is anthropogenic incidence. Contaminated soil belongs to waste and has to be removed in a proper way. is A special way of waste removal is soil decontamination. Its goal is to remove harmful substances and regain the original use of the soil.

Nosocomial infections, which do not often relate to the diseases are increasing nowadays. Clostridium difficile belongs to the frequent nosocomial infections and it is known as post-antibiotic colitis. The main reason of colitis is the usage of antibiotics, especially broad-spectrum antibiotics. The thesis is divided into the theoretical part and practical, as well. Theoretical part describes the division of the nosocomial infections, infection of the intestinal tract, anatomy, physiology of the intestines and infectious diarrhoeal diseases. The thesis is subsequently aimed to the clostridial infections and precautions against the spread of the disease. Practical part is aimed to the knowledge of the nurses, skills and attitude towards this issue.The thesis uses quantitative investigation and technique of the questionnaires, hidden observation of the nurses working on the selected wards and additional interviews with head nurses. The research was conducted in hospital in Tábor, a.s. The questionnaires were distributed on the surgery, orthopaedics, surgical JIP, ARO, ONP, infective ward, rehabilitative ward, TRN, cardio JIP, internal ward-cardio, internal ward-gastro. The thesis was formed from 143 questionnaires and 171 questionnaires were distributed. Hidden observation was made by head nurses from individual wards and it was logged to the relevant observation sheets.From existing findings we can say that there exist specifics of nursing care at the patient with the clostridium difficile. Among to these specifics we can cite the barrier nursing care where we can include the isolation of the patient, disinfection and hygiene of hands,using protectors, appropriate usage of laundries and infectious waste, location of the patient according to the epidemiological perpective and individualization of the tools for the patients. From another investigation ensue that the nurses keep barrier nursing care, superficial disinfecion, decontamination of the tools. From the results is evident that the nurses do not know the methods of the transmission of the clostridial infection. On the base of another investigations we have found out that the nurses do not know principles of the barrier nursing care. In conclusion is it possible to say that the nurses do not have so extensive information, that are essential for care for the patiens with clotridium difficile. In order to care for these patients in right way is neccessary to know principles of the barrier nursing care and keep them all. Keeping the principles of the barrier nursing care is crucial step in preventing the transmission nosocomial infections. The results will be provided to the officials of the individual hospital´s wards as an option of improvement in caring for the patiens with clostridial infection. The results were partially presented at a conference in Tabor´s hospital in May 2014. We recommend to re-train the staff of the hospital, which would be specifically aimed towards the principles of the barrier nursing care and towards the disinfection and decontamination in related to the nosocomial infections. On the base of these findings was made a proposal of the nursing care standard, which would specify and unite the care for the patiens with clostride infection. Subsequently, it would be apropriate to repeat the research in 1 2 years and than both researches compare together.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The aim of this work was to evaluate the efficiency of ozonification oxidation for horticulture submitted to pesticide decontamination, as well as to assess ozonification‟s effects on the physical-chemical properties and shelf time of the treated crops. Two experiments were carried out in 2015, using tomato and pepper crops. The experiments were performed in accordance with the recommendations for the crops. The crops were treated with two fungicides and two insecticides. In the tomato experiment, we applied the fungicide pyraclostrobin ® and the insecticide deltamethrin ®, while in the chili experiment we applied the fungicide and insecticide deltamethrin ® difenoconazole, using a sprayer pressurized with CO2 with a cone-type jet plane (XR TeeJet 11004). The treatments were the recommended dose, twice the recommended dose, and four times the recommended dose of the pesticides. In the tomato experiment, the fruit was harvested 3 hours after application, whereas in the chili experiment the pepper crop was held after the withdrawal period of the pesticide, and less treatment than the recommended dose was performed 24 hours before the withdrawal period. After harvesting, the samples were ozonized for 0, 10, 20, or 40 minutes and chromatographic analysis was conducted to measure the levels of pesticide degradation and to evaluate the physical and chemical qualities and shelf times of samples treated with ozone / O objetivo deste trabalho foi avaliar a eficiência do uso do processo de oxidação por ozonização na descontaminação de tomate e pimentão submetidas à aplicação de agrotóxicos, bem como avaliar as qualidades (físicos-químicas) e tempo de prateleira, pós ozonização. Foram realizados dois experimentos em 2015, utilizando as culturas de tomate e pimentão, conduzidos a campo. A implantação dos experimentos foi realizada de acordo com a recomendação para as culturas. Os tratamentos foram submetidos a dois fungicidas e dois inseticidas. No experimento com a cultura do tomate foi aplicado o fungicida piraclostrobina® e o inseticida deltametrina®, enquanto no experimento com a cultura do pimentão foi aplicado o fungicida difenoconazol e o inseticida deltametrina®, utilizado um pulverizador costal pressurizado com CO2, munido com barra com duas pontas do tipo cone jato plano (XR TeeJet 11004) . Os tratamentos foram formados pela aplicação da dose, duas vezes a dose e quatro vezes a dose recomendada dos agrotóxicos. No experimento com tomate a colheita dos frutos foi realizada três horas após a aplicação. Enquanto que, no experimento com pimentão a colheita foi realizada após o intervalo de segurança do agrotóxico, menos o tratamento com a dose recomendada foi realizado a colheita desrespeitando (24 horas antes) o intervalo de segurança. Após a colheita as amostras de frutos foram ozonizadas com os tempos de 0 (zero), 10 (dez), 20 (vinte) e 40 (quarenta) minutos e posteriormente foram realizadas as análises cromatográficas. Para medir os níveis de degradação dos agrotóxicos também foram determinadas as qualidades físicos- químicas e tempo de prateleira dos frutos sobre o efeito de tratamento com ozônio. No experimento com tomate a ozonização teve efeito significativo na redução do princípio ativo piraclostrobina, em 64% quando aplicada na dose recomendada em dez minutos de ozonização, a qualidade pós-colheita e o tempo de prateleira não apresentaram diferença em relação à testemunha. No experimento com pimentão a ozonização não teve efeito significativo, no entanto, numericamente atingiu uma redução de 45% no difenoconazol, no tratamento com dez minutos de ozonização na dose de 4 vezes acima da recomendada. As principais qualidades físico-químicas e tempo de prateleira se mantiveram semelhantes com a testemunha.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Constantly it s identified new areas contaminated by heavy metals. Among these metals, the copper, when it s in high concentrations in the soil can cause toxicity to the environment, being necessary the study of alternatives for the decontamination of contaminated areas. Thus, the aim of this study was to evaluate the initial development and tolerance of Senna multijuga and Erythrina crista-galli associated with the use of peat in soil contaminated with copper. To attend the objectives were conducted three experiments. The first involved the evaluation of growth, tolerance and accumulation of copper in plants of Senna multijuga and Erythrina crista-galli. In the second study evaluated the use of peat (200 mL of peat L-1 of soil) and Erythrina crista-galli in phytoremediation of soil contaminated with copper and the third evaluated the peat (200 mL of peat L-1 of soil) as ameliorating of copper in the soil and the potential of Senna multijuga as phytoremediation species. The results indicate that the copper doses reduced the morphological parameters of the plants of the species studied. The copper absorbed by plants was predominantly accumulated in the root system, being small its translocation to the shoot. Erythrina crista-galli and Senna multijuga don t presented as tolerant species to copper and had low potential metal fitoextrator the soil. The use of 200 ml of peat L-1 of soil is not as efficient as ameliorating of contamination with copper, but enabled increase in morphological parameters. / Constantemente são identificadas novas áreas contaminadas por metais pesados. Dentre estes, o cobre, quando em altas concentrações no solo, pode causar toxicidade ao meio ambiente, sendo necessário o estudo de alternativas para descontaminação de áreas contaminadas. Diante disso, o objetivo desse trabalho foi avaliar o desenvolvimento inicial e a tolerância de Senna multijuga e Erythrina crista-galli associada à utilização de turfa em solo contaminado com cobre. Para atender aos objetivos foram realizados três experimentos. O primeiro envolveu a avaliação do crescimento, tolerância e acúmulo de cobre em mudas de Senna multijuga e Erythrina crista-galli. No segundo trabalho avaliou-se o uso de turfa (200 mL de turfa L-1 de solo) e Erythrina crista-galli na fitorremediação de solo contaminado com cobre e, o terceiro avaliou-se a turfa (200 mL de turfa L-1 de solo) como amenizante de cobre no solo e o potencial da Senna multijuga como espécie fitorremediadora. Os resultados indicam que as doses de cobre reduziram os parâmetros morfológicos das mudas das espécies estudadas. O cobre absorvido pelas mudas foi acumulado predominantemente no sistema radicular, sendo pequena a sua translocação para a parte aérea. Erythrina crista-galli e Senna multijuga não se apresentaram como espécies tolerantes ao cobre e apresentaram baixo potencial fitoextrator do metal no solo. A utilização de 200 mL de turfa L-1 de solo não é eficiente como amenizante da contaminação com cobre, porém possibilitou aumento dos parâmetros morfológicos.

This paper reports the study of soils contaminated with mineral insulating oil in order to propose remediation processes that are best suited to them. The processes of soil treatment methods involve physical, chemical and biological, and in many cases necessary combination thereof. The proper study of the effects that can be caused by the contamination of the soil above a classification comprising aspects such as adsorption desorption processes, leaching, solubilization, among others. Contaminated soils were used in the Triangulo Mineiro region, the classification being conducted following the procedures described by the Brazilian Association of Technical Standards (ABNT), NBR 10004, NBR 10005, NBR 10006 and NBR 10007. From the obtained results it can be seen that the extent of contamination is limited to the area necessary to saturate the soil in the oil. Under the experimental conditions determined by Brazilian standards contamination is confined to this area, there are no processes and leaching significant. These results propose a great possibility that these remediation for contaminated soils can be implemented in the actual contaminated site without commitment and leaching processes that lead to environmental commitment. / O presente trabalho relata o estudo de solos contaminados com óleo mineral isolante com o objetivo de propor processos de remediação que sejam mais adequados aos mesmos. Os processos de tratamento do solo englobam métodos físicos, químicos e biológicos, sendo em muitos casos necessária a combinação dos mesmos. O estudo adequado dos efeitos que podem ser desencadeados pela contaminação do solo precede de uma classificação contemplando aspectos como forma de adsorção de dessorção, processos de lixiviação, solubilização entre outros. Foram utilizados solos contaminados da região do Triângulo Mineiro, considerando a classificação realizada através dos procedimentos descritos pela Associação Brasileira de Normas Técnicas (ABNT), NBR 10004, NBR 10005, NBR 10006 e NBR 10007. Pelos resultados obtidos pode ser verificado que a extensão da contaminação se restringe a área necessária para saturar o solo pelo óleo. Nas condições experimentais determinadas pelas normas brasileiras, a contaminação se restringe a esta área, não havendo processos de lixiviação e solubilização significativos. Tais resultados ensejam uma grande possibilidade de que a remediação para estes solos contaminados possa ser executada no próprio local contaminado sem comprometimento de processos de lixiviação e solubilização que levem ao comprometimento ambiental. / Doutor em Geografia