Orientador: Wirley Gonçalves Assunção / Coorientador: Cristiane Duque / Banca: Leonardo Perez Faverani / Banca: Valetin Adelino Ricardo Barão / Resumo: O objetivo desse estudo foi avaliar, in vitro, a corrosão em diferentes superfícies de Titânio comercialmente puro-Grau 4 (Ticp-G4) em função do efeito de biofilme bacteriano de Enterococcus faecalis. 57 discos foram utilizados divididos aleatoriamente de acordo com a superfície (n=19): Grupo I superfície usinada (USI); Grupo II superfície texturizada por duplo ataque ácido (D.A.) e Grupo III superfície texturizada por duplo ataque ácido e jateamento por zircônia (D.A.Zir). Para análise microbiológica (n=9), os discos foram posicionados em placas de 24 poços contendo cultura de Enterococcus faecalis à 1.5 x 105 células/mL em meio BHI caldo e incubados por 168 horas (7 dias) a 37° C. Após este período os discos foram removidos e as bactérias aderidas em sua superfície foram avaliadas por meio de contagem de Unidades Formadora de Colônias/mL (UFC/mL). Para análise corrosiva (n=5), novos espécimes foram inoculados da mesma forma em cultura de E. faecalis e após 7 dias, estes foram destinados ao ensaio eletroquímico por meio de espectroscopia de impedância eletroquímica (EIE), utilizando como eletrólitos BHI e biofilme e BHI caldo estéril. Os resultados foram submetidos à análise estatística: microbiológico (ANOVA com pós teste Tukey; 5%) e ensaio eletroquímico (ANOVA 2 Fatores e pós teste Tukey 5%). Na avaliação da contagem celular bacteriana, somente D.A.Zir (média 34 UFC/ml) diferiu de USI (média 16 UFC/ml) (p = 0,034), mas não de D.A. (24 UFC/ml). Na espectroscopia de impedân... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The objective of this study was to evaluate, in vitro, the corrosion in different surfaces of Titanium commercially pure-Grade 4 (Ticp-G4) as a function of the bacterial biofilm effect of Enterococcus faecalis. 57 disks were randomly divided according to the surface (n = 19): Group I machined surface (USI); Group IIsurface textured by double acid attack (D.A.) and Group III surface textured by double acid etching and zirconia (D.A.Zir). For microbiological analysis (n = 9), the discs were positioned in 24-well plates containing Enterococcus faecalis culture at 1.5 x 10 5 cells / ml in broth BHI medium and incubated for 168 hours (7 days) at 37 ° C. period the discs were removed and the bacteria adhered on its surface were evaluated by means of Colony Forming Units / mL (CFU / mL) counts. For corrosive analysis (n = 5), new specimens were inoculated in the same way in E. faecalis culture and after 7 days, they were destined to the electrochemical test by means of electrochemical impedance spectroscopy (IEE) using BHI and biofilm electrolytes and BHI sterile broth. The results were submitted to statistical analysis: microbiological (ANOVA with Tukey post test, 5%) and electrochemical test (ANOVA 2 Factors and post test Tukey 5%). In the evaluation of bacterialcell count, only D.A.Zir (mean 34 CFU / ml) differed from USI (mean 16 CFU / ml) (p = 0.034), but not D.A. (24 CFU / ml). In electrochemical impedance spectroscopy, for Nyquist and BODE, the D.A.Zir (Biofilm) group showed ... (Complete abstract click electronic access below) / Mestre

Les travaux présentés dans ce manuscrit ont permis dans un premier temps de développer avec succès deux nouvelles méthodes pour réaliser des cyclisations radicalaires 5-endo et dans un second temps d'illustrer le potentiel synthétique de la chimie radicalaire des xanthates, mise au point au sein du laboratoire, par son application à la synthèse de substances biologiquement actives. Dans la première partie, différentes approches permettant un accès aux γ-lactames par cyclisation radicalaire 5-endo sans l'utilisation de métaux lourds toxiques tel que l'hydrure de tributylétain ont été étudiées. L'originalité des deux systèmes développés réside dans l'oxydation du radical issu de la cyclisation. Ainsi, contrairement aux réactions induites par l'hydrure de tributylétain, différents γ-lactames insaturés peuvent être obtenus après déprotonation. Les travaux réalisés dans la deuxième partie ont permis l'élaboration de nombreux analogues des duocarmycines, qui ont fait l'objet de tests biologiques. Ces différents composés ont pu être préparés grâce à une synthèse convergente et efficace qui consiste à coupler un noyau alkylant avec un bras intercalant par formation d'une hydrazone. Différentes parties alkylantes ont été synthétisées rapidement grâce à l'application de la séquence d'addition-cyclisation radicalaire à partir de xanthate, précédemment développée au laboratoire. Une étude a également été menée pour tenter d'apporter des modifications à ces molécules. Enfin, nous avons préparé divers bras intercalants selon plusieurs méthodes. Nous avons ensuite couplé les différentes parties. Les molécules ainsi synthétisées ont révélé des activités cytotoxiques très prometteuses (IC50 ~ 0.5-1.2 µM). Cette étude prouve donc l'intérêt synthétique de la chimie radicalaire des xanthates.

In the last century, no less than five nobel prizes have been awarded for the construction of carbon-carbon bonds : The Grignard reaction (1912), the Diels-Alder reaction (1950), the Wittig reaction (1979), Olefin metathesis (2005) and palladium cross-coupling reactions (2011). The latter two are transition metal catalyzed transformations and their impact on the synthesis of pharmaceutically active compounds, bulk chemicals, fine chemicals, high tech materials as well as agricultural chemicals has been phenomenal. These reactions have changed the way the scientific community views the science of synthesis. Unlike palladium, gold has long been considered to be an expensive and inert metal and therefore, research on Au catalysis was scarse until the begining of the new millenium. Once the scientific community realized the treasure trove of reactivity that gold had to offer, the number of chemical transformations as well as total syntheses involving Au(I)/Au(III) catalysis has sky rocketed. A methodology initially developped by Toste and coworkers has shown that intramolecular addition of a silyl enol ether on alkynes proceeds via a 5-exo¬-dig¬ process. In the first part of this thesis, we will discuss how the ancilary ligand on Au(I) species can influence pathway selectivity for these cyclizations, therefore opening the door to selective 6-endo-dig cyclizations to generate fused carbocycles. With biological processes as well as other competing processes becoming ever more efficient, the future of chemical synthesis is threatened. If it is to survive, the focus of new chemical transformations will have to be on the cost and the greeness of the process. In the second part of this thesis, we will demonstrate how Ag(I) and Cu(I) complexes can offer even better 6-endo-dig¬ selectivity than analogous Au(I) complexes. Silver is about 56 times less expensive than gold, and copper is about 453 times less expensive than gold. Due to the greatly increased selectivity as well as the diminished cost of the catalysts, we have provided access to an attractive 6-endo-dig¬ cyclization process.

Few studies have quantified resting mouse muscle metabolism and even fewer studies have separated the contribution of sarcoplasmic reticulum (SR) Ca2+ pumping to resting metabolic rate. Furthermore, the studies that have attempted to quantify the contribution of Ca2+ pumping have used indirect methods to inhibit SR Ca2+ ATPase activity. The purpose of this study is to directly quantify resting muscle oxygen consumption and the contribution of SR Ca2+ pumping to resting oxygen consumption in mouse hindlimb muscles by using CPA to specifically inhibit Ca2+ pump activity in intact muscles at rest. The TIOX system was used to measure resting muscle VO2 of extensor digitorum longus (EDL) and soleus (SOL) muscles at 30oC and 20oC. C57BL mice aged 8-12 weeks were used with an average whole body mass of 23.8 g and EDL and SOL dry weights averaging 1.88 mg and 1.8 mg, respectively. All muscle VO2 measurements are expressed per gram dry weight. There were no differences (P>0.1) in resting muscle VO2 between EDL and SOL muscles at either 30oC (EDL, 2.05 µL/g/s; SOL, 2.27 µL/g/s) or 20oC (EDL, 0.62 µL/g/s; SOL, 0.71 µL/g/s). The average Q10 (3.1) was determined from EDL and SOL VO2 measures at 20oC and 30oC. The contribution of Ca2+ pumping by the sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) was measured at 30oC using a range of CPA concentrations (1-15 µM) . There was a concentration-dependent effect of CPA on oxygen consumption with increasing CPA concentrations up to 10 µM resulting in progressively greater reductions in muscle oxygen consumption. Specifically, 1, 5, 10, and 15 µM CPA caused an 11, 35.4, 49.5, and 50.3% reduction in VO2. There were no differences (P>0.1) between 10 and 15 µM CPA indicating that 10 µM CPA induces maximal inhibition of SERCA in isolated muscle preparations. The results indicate that the Ca2+ pumping by SERCA is responsible for ~50% of oxygen consumption in resting mouse EDL and SOL muscle. This is the first study to use a direct inhibitor of SERCA to quantify the contribution of Ca2+ cycling to resting oxygen consumption and therefore is a more accurate reflection of the actual contribution of SERCA to resting muscle oxygen consumption compared to previous findings. These results suggest that SERCA energy consumption accounts for a large portion of resting muscle metabolism and may represent a potential therapeutic target for metabolic alterations to oppose obesity.

Bacillus sp VLSH08 screened from sea wetland in Nam Dinh province produces an extracellular endo-1,4-beta-glucanase. According to the results of the classified Kit API 50/CHB as well as sequence of 1500 bp fragment coding for 16S rRNA gene of the Bacillus sp VLSH 08 strain showed that the taxonomical characteristics between the strain VLSH 08 and Bacillus amyloliquefaciene JN999857 are similar of 98%. Culture supernatant of this strain showed optimal cellulase activity at pH 5.8 and 60 Celsius degree and that was enhanced 2.03 times in the presence of 5 mM Co2+. Moreover, the gene encoding endo-1,4-beta-glucanase from this strain was cloned in Escherichia coli using pCR2.1 vector. The entire gene for the enzyme contained a 1500-bp single open reading frame encoding 500 amino acids, including a 29-amino acid signal peptide. The amino acid sequence of this enzyme is very close to that of an EG of Bacillus subtilis (EU022560.1) and an EG of Bacillus amyloliquefaciene (EU022559.1) which all belong to the cellulase family E2. A cocktail of enzyme containing this endo-1,4-beta-glucanase used for biomass hydrolysis indicated that the cellulose conversion attained to 72.76% cellulose after 48 hours. / Chủng vi khuẩn Bacillus sp VLSH08 được tuyển chọn từ tập hợp chủng vi khuẩn phân lập ở vùng ngập mặn tỉnh Nam Định có khả năng sinh tổng hợp enzyme endo-1,4-beta-glucanase ngoại bào. Kết quả phân loại chủng vi khuẩn Bacillus sp VLSH08 bằng Kit hóa sinh API 50/CHB cũng như trình tự gen mã hóa 16S rRNA cho thấy độ tương đồng của chủng Bacillus sp VLSH08 và chủng Bacillus amyloliquefaciene JN999857 đạt 98%. Dịch lên men của chủng được sử dụng làm nguồn enzyme thô để nghiên cứu hoạt độ tối ưu của enzyme ở pH 5,8 và nhiệt đô 60oC. Hoạt tính enzyme tăng 2,03 lần khi có mặt 5 mM ion Co2+. Đồng thời, gen mã hóa cho enzyme endo-1,4-betaglucanase cũng được tách dòng trong tế bào Escherichia coli sử dụng vector pCR 2.1. Gen mã hóa cho enzyme này có chiều dài 1500 bp, mã hóa cho 500 axit amin, bao gồm 29 axit amin của chuỗi peptid tín hiệu. So sánh cho thấy trình tự gen endo-1,4-beta-glucanase của chủng Bacillus sp VLSH08 có độ tương đồng cao với enzyme này của chủng Bacillus subtilis (EU022560.1) và của chủng Bacillus amyloliquefaciene (EU022559.1). Tất cả các enzyme nhóm này đều thuộc họ cellulase E2. Enzyme của chủng này cũng đã được phối trộn với các enzyme khác tạo thành cocktail để thủy phân sinh khối cho kết quả cellulose bị thủy phân 72,76% sau 48 giờ.

Few studies have quantified resting mouse muscle metabolism and even fewer studies have separated the contribution of sarcoplasmic reticulum (SR) Ca2+ pumping to resting metabolic rate. Furthermore, the studies that have attempted to quantify the contribution of Ca2+ pumping have used indirect methods to inhibit SR Ca2+ ATPase activity. The purpose of this study is to directly quantify resting muscle oxygen consumption and the contribution of SR Ca2+ pumping to resting oxygen consumption in mouse hindlimb muscles by using CPA to specifically inhibit Ca2+ pump activity in intact muscles at rest. The TIOX system was used to measure resting muscle VO2 of extensor digitorum longus (EDL) and soleus (SOL) muscles at 30oC and 20oC. C57BL mice aged 8-12 weeks were used with an average whole body mass of 23.8 g and EDL and SOL dry weights averaging 1.88 mg and 1.8 mg, respectively. All muscle VO2 measurements are expressed per gram dry weight. There were no differences (P>0.1) in resting muscle VO2 between EDL and SOL muscles at either 30oC (EDL, 2.05 µL/g/s; SOL, 2.27 µL/g/s) or 20oC (EDL, 0.62 µL/g/s; SOL, 0.71 µL/g/s). The average Q10 (3.1) was determined from EDL and SOL VO2 measures at 20oC and 30oC. The contribution of Ca2+ pumping by the sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) was measured at 30oC using a range of CPA concentrations (1-15 µM) . There was a concentration-dependent effect of CPA on oxygen consumption with increasing CPA concentrations up to 10 µM resulting in progressively greater reductions in muscle oxygen consumption. Specifically, 1, 5, 10, and 15 µM CPA caused an 11, 35.4, 49.5, and 50.3% reduction in VO2. There were no differences (P>0.1) between 10 and 15 µM CPA indicating that 10 µM CPA induces maximal inhibition of SERCA in isolated muscle preparations. The results indicate that the Ca2+ pumping by SERCA is responsible for ~50% of oxygen consumption in resting mouse EDL and SOL muscle. This is the first study to use a direct inhibitor of SERCA to quantify the contribution of Ca2+ cycling to resting oxygen consumption and therefore is a more accurate reflection of the actual contribution of SERCA to resting muscle oxygen consumption compared to previous findings. These results suggest that SERCA energy consumption accounts for a large portion of resting muscle metabolism and may represent a potential therapeutic target for metabolic alterations to oppose obesity.

Dermatan sulphate (DS) is a sulphated glycosaminoglycan (GAG) that is widely distributed as proteoglycan throughout the extracellular matrix and at cell surfaces where it plays an important role in many key biological processes. The intra-cellular catabolism of DS commences with endohydrolysis of the polysaccharide chains to oligosaccharides, which are then sequentially degraded from the non-reducing terminus by lysosomal exoenzymes to monosaccharides and inorganic sulphate for transport out of the lysosome and re-utilisation by the cell. Both endo-β-N-acetylhexosaminidase (Hyal-1 hyaluronidase) and endo-β-glucuronidase activities towards DS have been proposed. The present study was undertaken to: 1) determine the substrate specificities and sub-cellular locations of these endoglycosidase activities; and 2) compare endoglycosidase activities and substrate specificities in the mucopolysaccharidoses, where a defect in one of the lysosomal exoenzymes required to degrade DS results in the lysosomal accumulation of partially degraded DS oligosaccharide fragments. To this end, a series of oligosaccharide substrates designed to represent aspects of the physiological substrate was prepared, and an assay was developed to measure endoglycosidase activities and determine their substrate specificities by quantifying specific oligosaccharide products. Assay substrates rich in glucuronic acid (GlcA) or iduronic acid (IdoA) were prepared by limited chondroitinase ABC digestion of chondroitin sulphate A and DS, respectively. The resulting tetra-to hexadecasaccharides were separated by size-exclusion chromatography and characterised by electrospray ionisation-tandem mass spectrometry (ESI-MS/MS). These substrates, which were not susceptible to degradation by lysosomal exoenzymes, were then incubated with Chinese hamster ovary (CHO)-K1 cell homogenate (source of endoglycosidase activity), and the oligosaccharide products generated from the non-reducing end of the substrate were measured by ESI-MS/MS. Endo-β-N-acetylhexosaminidase and endohexuronidase activities were detected towards the oligosaccharide substrates, with both activities preferentially degrading the GlcA-rich substrates and only minor activity observed towards IdoA-rich substrate. The endo-β-N-acetylhexosaminidase activity had a minimum-sized substrate requirement of a hexasaccharide and was observed to sequentially remove tetrasaccharides from the non-reducing end of oligosaccharides, whereas the endohexuronidase activity had a minimum substrate of an octasaccharide, acted randomly and was comparatively low. The activities displayed the same acidic pH optimum and responded in the same manner to changes in buffer composition and substrate concentration, and to the presence of divalent cations, NaCl, detergent and protease inhibitors. Both activities were modestly affected by the hyaluronidase inhibitor, apigenin. Percoll density gradient sub-cellular fractionation confirmed that the activities were primarily in the lysosomes and late endosomes. The endo-β-N-acetylhexosaminidase and endohexuronidase activities detected here in CHO-K1 cells are consistent with the Hyal-1 and endo-β-glucuronidase enzymes described previously. These data suggest that Hyal-1 and endo-β-glucuronidase are predominantly lysosomal enzymes that act in concert to degrade the low-sulphate, GlcA-rich domains of DS, but are less active towards the highly sulphated regions containing IdoA. To test the hypothesis that endoglycosidase activities are altered in the mucopolysaccharidoses, an attempt was made to compare Hyal-1- and endo-β-glucuronidase-like activities and their substrate specificities in mucopolysaccharidosis (MPS)-affected and unaffected control skin fibroblasts. However, no activity was detected towards octa- to hexadecasaccharide substrates in control fibroblast homogenates, and in homogenates of MPS fibroblasts deficient in the lysosomal exoenzymes α-L-iduronidase and N-acetylgalactosamine-4-sulphatase, despite the fact that: 1) what appear to be the products of Hyal-1 and endo-β-glucuronidase activities towards endogenous DS could be detected in the lysosomes of the MPS cells by sub-cellular fractionation; and 2) the ESI-MS/MS assay was demonstrated sensitive enough to detect endoglycosidase activities in homogenates of a number of different mouse tissues (including whole skin). We hypothesise that this absence of detectable endoglycosidase activity in skin fibroblasts results from enzyme non-recognition of the exogenous assay substrates tested, and hence that these cells contain heretofore undescribed Hyal-1 and endo-β-glucuronidase isoforms with unique substrate specificities. In conclusion, the development of an ESI-MS/MS assay to measure the products of endoglycosidase activities has enabled the characterisation of these activities towards DS. This strategy may be useful for the future study of endoglycosidase activities towards a variety of other GAGs such as heparan sulphate, where particular oligosaccharide structures have been shown to possess unique biological activities. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1374435 / Thesis (Ph.D.) - University of Adelaide, School of Paediatrics and Reproductive Health, 2009

Submitted by Vinícius Lírio (vinicius.lirio@gmail.com) on 2014-08-05T00:22:38Z No. of bitstreams: 1 Tese - Vinícius_Lírio.pdf: 4176343 bytes, checksum: d8f60e7c70598d53b51d5a726bf2824b (MD5) / Approved for entry into archive by Ednaide Gondim Magalhães (ednaide@ufba.br) on 2014-08-05T11:37:19Z (GMT) No. of bitstreams: 1 Tese - Vinícius_Lírio.pdf: 4176343 bytes, checksum: d8f60e7c70598d53b51d5a726bf2824b (MD5) / Made available in DSpace on 2014-08-05T11:37:19Z (GMT). No. of bitstreams: 1 Tese - Vinícius_Lírio.pdf: 4176343 bytes, checksum: d8f60e7c70598d53b51d5a726bf2824b (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / O presente estudo apresenta uma série de reflexões deflagradas no contato com a poética do espetáculo DÔ, do Bando de Teatro Olodum com direção do mestre de butô Tadashi Endo. Desenvolvido durante o ano de 2012, esse processo criativo constituiu-se como espaço-tempo de entrecruzamento e atualização de matizes culturais diversos, por meio de um projeto dinâmico que hibridizou princípios de uma prática específica de butô, a de Tadashi Endo, e os traços de uma teatralidade profundamente marcada pelas culturas que a atravessam, aquela do Bando. Considerando essa rede transcultural, as articulações desenvolvidas no decorrer dessa tese sinalizam alguns traços e desdobramentos daquilo que se reconheceu, aqui, como Poéticas Híbridas. Para tanto, foi realizada uma abordagem dessa poética enquanto ato criativo em processo, a partir da apreciação dos rastros da criação, sua maior parte em registro audiovisual, entrevistas com os sujeitos dessa obra, materiais publicados pelos mesmos e expectação do espetáculo em si, a fim de identificar os fenômenos implicados nessa montagem. Junto a isso, os pensamentos de alguns estudiosos das Artes Cênicas, dos campos das culturas e Ciências Humanas, bem como, filósofos, foram basilares para a abordagem e os desdobramentos reflexivos implicados nesta tese, a citar: Christine Greiner (1998; 2000; 2005), no que tange aos estudos do corpo e do butô; Sônia Azevedo (2009) e Lúcia Romano (2008), no que se refere às discussões acerca do lugar do corpo e de seus fenômenos na cena; Stuart Hall (2003a; 2003b; 2006), Homi Bhabha (1996; 2010) e Nestor G. Canclini (1982; 2011), cujos estudos versam sobre os processos identitários e culturais no contexto dos campos híbridos e das nações pós-modernas; Michel Maffesoli (2008; 2007), cujo pensamento sugere diferentes formas de lidar com os fenômenos socioculturais; estudiosos como Jean-Pierre Ryngaert (1995; 1998), Patrice Pavis (2010a; 2010b; 2008) e Sílvia Fernandes (2010), que trazem reflexões importantes para pensar os projetos poéticos na cena contemporânea; por fim, dentre outros, vale citar o nome de Cecília de Almeida Salles (2008; 2009), cujos estudos contribuíram em muito para a abordagem metodológica dessa pesquisa. As articulações desenvolvidas no corpo dessa tese foram desencadeadas a partir de questões relacionadas à poética em si, isto é, seus processos e procedimentos, seu espaço-tempo e os sujeitos nela envolvidos, com o intuito de construir uma rede de pensamentos considerando o modo como foram estabelecidas as conexões, os agenciamentos e mediações entre os desejos, discursos e culturas envolvidas no processo criativo. Esse estudo acabou por mapear os vetores (procedimentos, movimentos, memórias, discursos, práticas, etc.) que, articulados, constituíram essa poética do Bando e de Tadashi. Nesse caminho, foram realizadas uma série de reflexões sem, no entanto, encerrá-las, o que faz deste um estudo de ideias provisórias. / ABSTRACT: This study presents a series of reflections triggered by contact with the DÔ poetics, spectacle of Bando de Teatro Olodum under the direction of the Butoh master Tadashi Endo. Developed during the year 2012, this creative process was established as a space-time of crisscross and update of cultural nuances various through a dynamic project that hybridized principles of a specific practice of Butoh, the one of Tadashi Endo, and traces of a theatricality deeply marked by cultures that cross it, that one of Bando. Considering this transcultural space, the articulations developed during this thesis indicate certain traits and developments of what is recognized here as Hybrid Poetics. Therefore, an approach of this poetic as a creative act in process, from the enjoyment of creating tracks, mostly in audiovisual recordings, interviews with the participants in that work, materials published by them and expectation of the show itself, to identify the phenomena involved in this poetic. In addition, some theoretical thoughts of Performing Arts, fields of cultures and Humanities, as well as philosophers, were fundamental to the approach and reflective developments implicated in this thesis, among them: Christine Greiner (1998, 2000, 2005), in regard to studies of the body and Butoh; Sônia Azevedo (2009) and Lúcia Romano (2008), in relation to discussions on the place of the body and its phenomena in the perfomance; Stuart Hall (2003a, 2003b, 2006), Homi Bhabha (1996, 2010) and Nestor G. Canclini (1982, 2011), whose studies are related to the identity and cultural processes in the context of hybrid fields and post-modern nations; Michel Maffesoli (2008, 2007), whose ideas suggests different ways of dealing with the socio-cultural phenomena; scholars such as Jean-Pierre Ryngaert (1995, 1998), Patrice Pavis (2010a, 2010b, 2008) and Sílvia Fernandes (2010), which bring important insights to think the poetic projects in the contemporary theatre; Finally, among others, it is worth mentioning the name of Cecilia de Almeida Salles (2008; 2009), whose studies have contributed much to the methodological approach of this research. The articulations developed in the body of this thesis were triggered from issues related to the poetic itself, in other words, its processes and procedures, its space-time and the participants involved in it, aiming to build a network of thoughts considering how connections, negotiations and mediations between the desires, discourses and cultures involved were established in the creative process. This study turned out to map the vectors (procedures, movements, memories, discourses, practices, etc.) which, articulated, constituted this poetics of Bando and Tadashi. In this way, a series of reflections were performed without, however, close them, which makes this a study of provisional ideas.

Submitted by Barroso Patrícia (barroso.p2010@gmail.com) on 2014-08-21T01:51:35Z No. of bitstreams: 1 GESTEIRA, Mária de Fátima Malvar.pdf: 14332589 bytes, checksum: fab60e995789cf4073cfcc1575ba30b2 (MD5) / Made available in DSpace on 2014-08-21T01:51:35Z (GMT). No. of bitstreams: 1 GESTEIRA, Mária de Fátima Malvar.pdf: 14332589 bytes, checksum: fab60e995789cf4073cfcc1575ba30b2 (MD5) / Durante o preparo químico-cirúrgico do canal radicular, a efetividade dos instrumentos endodônticos e das soluções químicas auxiliares empregadas durante a modelagem, limpeza e desinfecção sustentam o sucesso do tratamento endodôntico. Ao seu lado, agentes quelantes têm sido utilizados ao final da instrumentação com o objetivo de remover da superfície dentinária um extrato de aparência amorfa, superfície irregular e granulosa, denominada camada de magma dentinário. Este estudo se propôs a avaliar, in vitro, por meio da Microscopia Eletrônica de Varredura, o efeito do EDTA a 17% / NaOCl 1% e do BioPure™ MTAD® na remoção da camada de magma dentinário, em dentes preparados química e cirurgicamente com o auxílio de Endo-PTC® / NaOCl 1% e de NaOCl 1%. Incisivos e caninos superiores humanos unirradiculares (n=50) foram distribuídos por sorteio em quatro Grupos Experimentais (n=10/grupo) e dois Grupos Controle Positivo (n=5/grupo). Nos grupos Experimentais 1 e 3 e no Grupo Controle Positivo 1, a instrumentação realizou-se com o auxílio da associação Endo- PTC® / NaOCl a 1%. Nos Grupos Experimentais 2 e 4 e no Grupo Controle Positivo 2, a instrumentação realizou-se com NaOCl 1%. Concluída a instrumentação, procedeu-se à irrigação final, nos Grupos Experimentais 1 e 2, com 10 mL de EDTA a 17% / NaOCl 1% por 1 minuto e, nos Grupos Experimentais 3 e 4, com BioPure™ MTAD® conforme orientação do fabricante. Em seguida, os dentes foram clivados, e a limpeza da superfície dentinária apical foi avaliada através da Microscopia Eletrônica de Varredura. Após tratamento estatístico, o resultado deste estudo revelou que as soluções de EDTA a 17% / NaOCl 1% mostraram-se mais eficazes na remoção da camada de magma dentinário no terço apical radicular do que o BioPure™ MTAD® (p<0,05), concluindo-se, porém, que nenhuma das substâncias testadas foi capaz de remover toda a camada de magma dentinário nessa região. O creme Endo-PTC® não contribuiu, do ponto de vista estatístico, para dificultar a remoção da camada de magma dentinário. A 6 mm do ápice, as superfícies dentinárias tratadas com EDTA a 17% / NaOCl a 1% apresentaram superfícies dentinárias mais limpas do que a 3 mm.

Orientador: Idelmo Rangel Garcia Júnior / Banca: Roberta Okamoto / Banca: Luiz Antônio Salata / Banca: Antonio Carlos Guastaldi / Banca: Paulo Sérgio Perri de Carvalho / Resumo: A estabilidade primária na instalação de implantes dentários é uma das determinantes principais no processo de osseointegração. Algumas situações clínicas impossibilitam o travamento adequado do implante. A diástase provocada por sobrefresagem ou implantações pós-exodônticas indicam o uso de materiais para ocupar este espaço. O objetivo deste estudo foi analisar o uso de copolímero a base de ácido poliláctico na estabilidade primária de implantes osseointegráveis, realizando uma avaliação microscópica através de fluorocromos. Para isso, foram utilizados 14 cilindros de Ti cp GII instalados na tíbia de ratos, divididos em: Grupo Implante Travado (GIT) e Grupo Implante e Biomaterial (GIB), nos períodos de 7, 15 e 21 dias. Resultado: Os implantes suportaram 3N.cm de torque reverso e não foram encontradas diferenças estatisticamente significantes, nos períodos finais, entre as médias das áreas ao redor do implante. O copolímero de PLA/PGA proporcionou o posicionamento dos implantes e não obstou a deposição óssea. / Abstract: The primary stability in the installation of dental implants is one of the main prerequisite in the osseointegration process. Some clinical situations disable the adequate stability of the implantation. A gap occurred between the bone and the implant provoked by overdrilling or tooth extractions sockets implantations indicates the use of biomaterials to occupy this space. The aim of this study was to analyze the use of PLA/PGA copolymer in the primary stability of osseointegrated implants, through removal torque test and a microscopic evaluation with fluorescent bone markers. For this, 14 cylinders of Ti cp GII had been installed in the tibia of rats, divided in: Group Implant with Stability (GIT) and Group Implant and Biomaterial (GIB), in the periods of 7, 15 and 21 days. Result: The implantations had supported 3N.cm of removal torque and had not been found differences statistically significant, in the final periods, between the averages of the areas of the periphery of the implants. The PLA/PGA copolymer kept the implants position and did not hinder the bone deposition. / Doutor