O efeito dos hormônios glicocorticóides (GC) de suprimir o crescimento celular é exercido através de cascatas celulares nas quais a transcrição de genes de resposta primária, mediada direta ou indiretamente pelo receptor de GC, regulam a transcrição e a atividade de um conjunto de genes, incluindo fatores importantes para a progressão no ciclo celular. Entretanto, a conexão funcional entre diversos dos genes ativados ou inibidos por GC e a inibição da proliferação de determinados tipos celulares ainda não é completamente conhecida. Nosso laboratório isolou a variante ST1, a partir da linhagem C6 de glioma de rato, utilizando-a como modelo de estudo do mecanismo de ação de GC como agentes anti-tumorais. O tratamento com GC confere, às células ST1, um crescimento totalmente dependente de soro e ancoragem, morfologia em cultura semelhante à de fibroblastos normais e incapacidade de gerar tumor em camundongos da linhagem \"nude\", caracterizando uma completa reversão fenotípica tanto in vitro como in vivo. Como abordagens para o entendimento do mecanismo molecular da ação de GCs, o laboratório vem buscando a clonagem de produtos gênicos diferencialmente expressos através do uso de técnicas que permitam a análise da abundância relativa dos mRNAs, e mais recentemente, empregando-se a análise da abundância relativa das proteínas através da eletroforese bidimensional (2D-PAGE). As seqüências isoladas por estas metodologias são alvos potenciais para uma posterior análise funcional. Os objetivos gerais deste trabalho foram: a) identificar genes que estão diferencialmente expressos durante a reversão fenotípica das células ST1 induzida por GC, através da análise proteômica (2D-PAGE e espectrometria de massas) e b) determinar a função de um dos genes (Ciclina G) identificado anteriormente no laboratório como sendo induzido durante o tratamento das células ST1 com GC. A metodologia empregada se baseou na comparação dos perfis 2D de proteínas nucleares das células ST1 tratadas ou não (controle) com GC por 5 e 24h. Após análise de imagem, 33 polipeptídios foram considerados como diferencialmente representados após 5h de tratamento, 16 dos quais foram também identificados após 24h de tratamento. Seis destes polipeptídios foram identificados através da análise dos seus perfis de digestão tríptica (PMF). Evidências obtidas por ensaios de Western blot sugerem que um destes polipeptídeos, a Anexina 2 (ANX2), possui a sua localização sub-celular modulada pela ação de GC nas células ST1. A análise do papel da Ciclina G na reversão fenotípica tumoral-normal das células ST1 induzida por GC, foi feita através da sua super-expressão nestas células, utilizando um sistema retroviral, e avaliando-se os efeitos desta super-expressão sobre a resposta das células ST1 a GC, através de ensaios de curva de crescimento e citometria de fluxo. Os dados sugerem que a super-expressão da Ciclina G nas células ST1 intensifica e prolonga o efeito de GC nestas células. / The glucocorticoid (GC) growth suppression response is controlled through cellular cascades in which the transcription of primary response genes regulates the expression and activity of a diverse set of genes including important factors for cell cycle progression. However, the functional connection between the GC-regulated transcriptional events and cell cycle arrest of determined cells is poorly understood at the molecular level. In this context, our lab has isolated the ST1 variant of the C6 rat glioma cell line to study the mechanism of action of GC as anti-tumor agents. GC treatment leads ST1 cells to a dramatic tumoral to normal phenotypic reversion, characterized by inhibition of their growth rate in monolayer, loss of their ability to form colonies in semi-solid medium and to induce tumor formation in nude mice, and morphological changes (flattening). As part of the strategy to understand the anti-tumor action of GC, differentially represented proteins, associated with the phenotypic reversion displayed by ST1 cells have been isolated through mRNA-based blind cDNA cloning and, more recently, by two dimensional electrophoresis (2D-PAGE). The sequences isolated by these two methodologies are potential targets for functional analysis. In the present study, we aimed at a) identifying genes which are differentially expressed during the GC-induced phenotypic reversion of ST1 cells, using the proteomic approach (2D-PAGE and mass spectrometry) and b) determining the functional role of a gene (Cyclin G) which had previously being identified as being induced during GC-treatment of the ST1 cells. The analytical methodology used relied on the comparison of sets of 2D nuclear protein profiles of ST1 cells, maintained in the absence (control) and in the presence of GC for 5 and 24h. After image analysis and visual validation, 33 polypeptides were considered as differentially represented 5 h after treatment, 16 of which were also differentially represented after 24 h. Six of those polypeptides were identified by peptide mass fingerprinting (PMF). Evidence obtained by Western blot analysis indicates that one of those polypeptides, Annexin 2 (ANX2), has its sub-cellular location modulated by GC-treatment of ST1 cells. The role of Cyclin G in the tumoral to normal phenotypic reversion induced by GC in ST1 cells was analyzed by over-expression of Cyclin G using a retroviral system and evaluation of the effects of this over-expression over ST1 cells response to GC, by growth curve and flow cytometry assays. The data suggest that Cyclin G over-expression leads to a more intense and prolonged effect of GC on ST1 cells.

Molibdênio é um elemento traço envolvido na fixação de nitrogênio, enxofre e carbono. Oligopeptídeos estão envolvidos na nutrição bacteriana e diversos outros processos de sinalização intercelular. O objetivo do presente estudo foi investigar o papel funcional das proteínas ligadoras dos sistemas de captação de molibdato (ModA) e oligopeptídeo (OppA) em Xanthomonas axonopodis pv. citri em condições in vitro e in vivo. O mutante ModA mostrou uma produção diminuída de goma xantana, alteração do biofilme e adesão prejudicada em condições in vitro. In vivo a interação do mutante modA mostrou alterações nas lesões causadas em folhas de grapefruit possivelmente resultado da baixa expressão do gene gumB. O mutante na proteína OppA apresentou células mais co-agregadas alterando a estrutura do biofilme e consequentemente diminuindo sua capacidade de adesão. In vivo, a linhagem mutante não alterou o fenótipo de patogenicidade, mas a sua capacidade de crescimento foi afetada no início da fase estacionária sugerindo que o sistema Opp desempenha um papel nutricional. / Molybdenum is a trace element involved in nitrogen fixation, sulfur and carbon. Oligopeptides are involved in bacterial nutrition and several other intercellular signaling processes. The aim of this study was to investigate the functional role of binding proteins of molybdate (ModA) and oligopeptide (OppA) uptake systems of Xanthomonas campestris pv. citri in in vitro and in vivo conditions. ModA mutant showed a decreased production of xanthan gum, altered biofilm and adhesion impaired in vitro conditions. In vivo ModA mutant interaction showed changes in injuries on leaves of grapefruit possibly due to low expression of the gumB gene. The OppA mutant showed more cells co-aggregated by changing the structure of the biofilm and consequently reducing their capacity to adhere. In vivo, the mutant strain did not modify the phenotype of pathogenicity, but its ability for growth was affected at the early stationary phase suggesting that the Opp system plays a nutritional role.

Orientador: Laura Maria Mariscal Ottoboni / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-25T23:22:56Z (GMT). No. of bitstreams: 1 Rodrigues_VivianeDrumond_D.pdf: 5631358 bytes, checksum: 6072655858120e417b92336ddd538828 (MD5) Previous issue date: 2014 / Resumo: O conhecimento acerca da diversidade microbiana associada a ambientes de mina é limitado, apesar da importância que alguns micro-organismos podem ter no processo de biolixiviação e biorremediação ambiental. Adicionalmente, micro-organismos que vivem em condições inóspitas, como os diferentes ambientes de mina, vêm despertando interesse cada vez maior por possuírem enzimas de interesse industrial. Neste sendido, a análise da biodiversidade funcional e estrutural de micro-organismos presentes em ambientes de mina é de fundamental importância para entender a estrutura e a complexidade das comunidades microbianas em ambientes extremos. Neste trabalho a diversidade microbiana foi analisada em diversos ambientes da mina de cobre do Sossego, localizada em Canaã dos Carajás, sudeste do Pará por abordagens dependentes e independentes de cultivo. A composição taxonômica associada a ambientes da mina do Sossego: taludes (estruturas geotécnicas) e entorno da drenagem dos depósitos de Sossego (T-SO1, T-SO2, ED-SO1, ED-SO2) e Sequeirinho (T-SE1, T-SE2, ED-SE1, ED-SE2) foi avaliada por pirosequenciamento do gene de rRNA 16S. Os resultados indicaram que a comunidade de bactérias de talude é distinta do entorno da drenagem e o conteúdo de matéria orgânica e maior disponibilidade de água foram os principais fatores para as diferenças. Os principais táxons responsáveis pelas diferenças foram Acidobacteria, Chloroflexi, Gammaproteobacteria e Firmicutes. Por meio de técnicas dependentes de cultivo, 64 bactérias heterotróficas foram isoladas a partir das amostras SO5, SO6, SO7 e SO9. Estes isolados foram identificados e avaliados quanto à capacidade de produção de enzimas (hidrolases, monoxigenases, sulfoxidases e betalactamase) e compostos (sideróforos, biossurfactantes e antimicrobianos). Foram identificadas bactérias afiliadas aos seguintes gêneros: Acidovorax, Acinetobacter, Brevundimonas, Cupriavidus, Curtobacterium, Kocuria, Lysinibacillus, Pseudomonas, Roseomonas, Ralstonia, Stenotrophomonas e Bacillus, sendo o último respresentado por 43 isolados. Com relação à triagem funcional, 95% das bactérias foram capazes de produzir sideróforos, 58% biossurfactantes, 69% betalactamases, 50% antimicrobianos, 53% proteases, 75% esterases, 20% monoxigenases e três isolados (SO5.4, SO5.9 e SO6.2) apresentaram oxidação seletiva para sulfetos orgânicos. A partir de amostras de drenagem (SO5, SO6 e SO7) foram obtidos consórcios de micro-organismos oxidantes de ferro. Estes consórcios foram testados com relação à capacidade de biolixiviação da calcopirita e foram mais eficientes para a dissolução do cobre do que Acidithiobacillus ferrooxidans LR. A identificação dos micro-organismos presentes nos consórcios foi realizada por eletroforese em gel de gradiente desnaturante (DGGE) e as bandas mais evidentes foram classificadas em Bacillus sp., Delftia sp., Phenylobacterium sp. e Methylobacterium sp. A comunidade de bactérias na mina de cobre do Sossego foi diversa e complexa. Estes resultados mostram um inventário da microbiota em diferentes ambientes da mina do Sossego e as enzimas e compostos obtidos destas bactérias poderão ser utilizadas em processos e tecnologias que permitam a recuperação de metais, como a biolixiviação e biorremediação ou em outras aplicações industriais / Abstract: The knowledge concerning microbial diversity associated with mine environments is limited, despite the importance that some microorganisms can have on environmental bioremediation and bioleaching process. Additionally, microorganisms that live in inhospitable conditions, such as different mine environments, have attracted growing interest because they could have enzymes with industrial applications. In this way, structural and functional biodiversity analysis in mine environments is an important issue to understand the structure and complexity of the microbial communities in extreme environments. The present work shows a microbial diversity analyses in some cooper mine environments of Sossego Mine localized in Canaã dos Carajás mineral province, Pará state, Brazil. The bacterial taxonomic composition associated with Sossego cooper mine: slopes (geotechnical structures) and surrounding drainage of Sossego and Sequeirinho deposits was evaluated using pyrosequencing of 16S rRNA gene. The results indicated slope bacterial community differs from surrounding drainage and organic matter content and higher water availably were the main factors of these differences. The foremost taxons accountable by those differences were Acidobacteria, Chloroflexi, Gammaproteobacteria and Firmicutes. Sixty four bacteria were isolated using culture-dependent methods from SO5, SO6, SO7 and SO9 samples. These bacteria were identified and evaluated concerning the capability of enzyme production (hydrolase, betalactamase, monooxygenase and sulphoxidases) and compounds (siderophore, biosurfactants and antimicrobials). It was identified bacteria related with the followed genera: Acidovorax, Acinetobacter, Brevundimonas, Cupriavidus, Curtobacterium, Kocuria, Lysinibacillus, Pseudomonas, Roseomonas, Ralstonia, Stenotrophomonas and Bacillus, the last one showed 43 isolates. In relation with functional screening, 95% of bacteria were capable to produce siderophores, 58% to produce biosurfactants, 69% betalactamases, 50% antimicrobials, 53% proteases, 75% sterases, 20% monooxygenases and three strains (SO5.4, SO5.9 and SO6.2) exhibited selective oxidation for organic sulphides. Iron oxidizing microorganism consortia were obtained from drainage samples and were tested according with its ability for bioleaching of chalcopyrite. The consortia obtained from SO5, SO6, and SO7 samples were more efficient than Acidithiobacillus ferrooxidans LR regarding bioleaching of copper from chalcopyrite. The identification of microorganism presented in the consortia was performed using DGGE technique and the more evident bands were classified as Bacillus sp., Delftia sp., Phenylobacterium sp. and Methylobacterium sp. The bacterial community in Sossego cooper mine was diverse and complex. These results showed a microbiota inventory in distinct mine environments and enzymes and compounds obtained from those bacteria could be used in new processes and technologies that allow to recovery metals as bioleaching, bioremediation or others industrial applications / Doutorado / Genetica de Microorganismos / Doutora em Genética e Biologia Molecular

Orientador: Fernando Roberto Martins / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-23T02:32:50Z (GMT). No. of bitstreams: 1 VanMelis_Juliano_D.pdf: 2552550 bytes, checksum: 8227a941fa221a10cce8b272ae92449f (MD5) Previous issue date: 2013 / Resumo: Apesar da importância que as trepadeiras apresentam em florestas tropicais, estudos sobre a montagem da comunidade de lianas (trepadeiras lenhosas e sublenhosas) que investiguem desde a contribuição dos fatores abióticos e bióticos até fatores intrínsecos (coexistência entre indivíduos) são escassos. O objetivo geral desta tese é pesquisar a estruturação da comunidade das espécies de lianas em uma Floresta Estacional Semidecídua (FES), investigando (1) a importância relativa dos fatores ambientais e espaciais para diferentes espécies de lianas, (2) a estruturação filogenética da comunidade de trepadeiras em diferentes ambientes, e (3) os efeitos diretos ou mediados das árvores e arbustos para o número de espécies e indivíduos de trepadeiras. Mostramos que (1) grande parte da variação na composição de espécies de lianas em uma FES é devido a fatores não investigados (fatores estocásticos) e o espaço (autocorrelação espacial). Portanto, concluímos que os maiores determinantes na variação da composição de espécies de lianas em uma FES é a aleatoriedade (sendo reflexo da variação estocástica das populações) e a limitação por dispersão (demonstrada pela alta autocorrelação espacial). No segundo capítulo (2), encontramos que uma maioria discreta das parcelas apresentou maior aproximação filogenética do que o esperado ao acaso na comunidade de trepadeiras amostrada. Houve pouca influência de variáveis relacionadas à dinâmica florestal na variação da aproximação filogenética, sendo que áreas com árvores mais altas e maior proporção de árvores do presente apresentavam maior aproximação filogenética que outras áreas. Concluímos que em áreas de dossel mais baixo e menor proporção de árvores do presente (clareiras) não apresentam menor sinal filogenético, pois todas as espécies de lianas apresentariam potencial de existirem nestas áreas, enquanto que nas áreas de floresta madura haveria a existência de filtros ambientais para a existência de poucos ramos filogenéticos. Por último (3), encontramos que os atributos da comunidade de árvores e arbustos são fatores importantes na variação dos atributos da comunidade de lianas, sendo parte dele decorrente do distúrbio no dossel. Mas o distúrbio no dossel como fator direto é mais importante na variação da abundância e número de espécies de lianas em uma Floresta Estacional Semidecídua / Abstract: Despite the fact that climbing plants present in tropical forests, studies which investigate the contribution of abiotic and biotic factors or intrinsic factors (coexistence between individuals) on community assembly of lianas (woody and sub-woody climbers) are scarce. The overall objective of this thesis is to research the community structure of liana species in a Seasonal Semideciduous Forest (SSF), investigating (1) the relative importance of environmental and spatial factors on community assembly of lianas, (2) the phylogenetic structure of climbing plants community along the forest development (treefall gaps to old-growth forest), and (3) the direct or indirect effects of trees and shrubs for the number of species and individuals of climbing plants. We show that (1) much of the variation in species composition of lianas in a SSF is due to stochastic factors and space. Therefore, we conclude that the major determinants of variation in lianas' species composition in a TSF are stochastic variance of populations, shown by the unexplained factors, and dispersion limitation, shown by spatial autocorrelation. In the second chapter (2), we found that a slight majority of the sample plots showed cluster phylogenetic structure in the climbing plants community. There was a slight influence of variables related to forest dynamics in the variation of the phylogenetic structure, and areas with tall trees and higher proportion of present trees had higher values of clustering in phylogenetic structure than other areas. We conclude that in areas of lower canopy and smaller proportion of present trees (treefall gaps) showed few phylogenetic branches, since all species of climbing plants would be existing in these areas, while areas of old-growth forest would demonstrate environmental filters for the climbing plants. Finally, we also found (3) that the community of trees and shrubs' attributes (abundance and species richness) are important factors in the variation of attributes liana community (species richness and abundance), being part of it due to the canopy disturbance. But canopy disturbance was the more important direct factor in variance of abundance and species richness of lianas in a Seasonal Semideciduous Forest / Doutorado / Doutor em Biologia Vegetal

Orientador: Aluísio de Souza Pinheiro / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Matemática, Estatística e Computação Científica / Made available in DSpace on 2018-08-19T09:39:03Z (GMT). No. of bitstreams: 1 Kist_Airton_D.pdf: 4639620 bytes, checksum: 2a0cc586e73dd5d71aa0eacf07be101d (MD5) Previous issue date: 2011 / Resumo: O problema de estimação funcional vem sendo estudado de formas variadas na literatura. Uma possibilidade bastante promissora se dá pela utilização de bases ortonormais de wavelets (ondaletas). Essa solução _e interessante por sua: frugalidade; otimalidade assintótica; e velocidade computacional. O objetivo principal do trabalho é estender os testes do modelo FANOVA de efeitos fixos, com erros i.i.d., baseados em ondaletas propostos em Abramovich et al. (2004), para modelos FANOVA de efeitos fixos com erros dependentes. Propomos um procedimento iterativo tipo Cocharane-Orcutt para estimar os parâmetros e a função. A função é estimada de forma não paramétrica via estimador ondaleta que limiariza termo a termo ou estimador linear núcleo ondaleta. Mostramos que, com erros i.i.d., a convergência individual do estimador núcleo ondaleta em pontos diádicos para uma variável aleatória com distribuição normal implica na convergência conjunta deste vetor para uma variável aleatória com distribuição normal multivariada. Além disso, mostramos a convergência em erro quadrático do estimador nos pontos diádicos. Sob uma restrição é possível mostrar que este estimador converge nos pontos diádicos para uma variável com distribuição normal mesmo quando os erros são correlacionados. O vetor das convergências individuais também converge para uma variável normal multivariada / Abstract: The functional estimation problem has been studied variously in the literature. A promising possibility is by use of orthonormal bases of wavelets. This solution is appealing because of its: frugality, asymptotic optimality, and computational speed. The main objective of the work is to extend the tests of fixed effects FANOVA model with iid errors, based on wavelet proposed in Abramovich et al. (2004) to fixed effects FANOVA models with dependent errors. We propose an iterative procedure Cocharane-Orcutt type to estimate the parameters and function. The function is estimated through a nonparametric wavelet estimator that thresholded term by term or wavelet kernel linear estimator. We show that, with iid errors, the individual convergence of the wavelet kernel estimator in dyadic points for a random variable with normal distribution implies the joint convergence of this vector to a random variable with multivariate normal distribution. Furthermore, we show the convergence of the squared error estimator in the dyadic points. Under a restriction is possible to show that this estimator converges in dyadic points to a variable with normal distribution even when errors are correlated. The vector of individual convergences also converges to a multivariate normal variable / Doutorado / Estatistica / Doutor em Estatística

Dans cette thèse, nous avons étudié l'influence de plusieurs variants génétiques situés dans les régions chromosomiques 5q31-q33, 6p21, et 17p12, pour lesquelles une liaison génétique avec des phénotypes de paludisme a été montrée.Les gènes NCR3 et TNF, qui sont situés dans la région chromosomique 6p21, ont été associés au paludisme dans une population vivant au Burkina Faso. Nous avons répliqué ces études dans une population congolaise afin deconfirmer les associations des polymorphismes avec les accès palustres simples et la parasitémie symptomatique. Nos résultats montrent que le polymorphismeNCR3-412 est associé avec les accès palustres simples au Congo, et que les polymorphismes TNF-308, TNF-244, et TNF-238 sont associés avec les accès palustres simples ou la parasitémie symptomatique. En outre, nos analyses bioinformatiques suggèrent que les polymorphismes TNF-244 et TNF-238 agissent en synergie pour modifier le site de fixation pour au moins un facteur de transcription.Les deux gènes HS3ST3A1 et HS3ST3B1, qui sont situés dans la région chromosomique 17p12, sont impliqués dans la biosynthèse des heparanes sulfates. Dans cette étude, nous avons étudié l'association d’un polymorphisme situé dans le promoteur de HS3ST3A1 avec les accès palustres simples et la parasitémie symptomatique, et n’avons détecté aucune association. Nous avons étudié en outre le gène NDST1, situé dans la région chromosomique 5q31-q33, et qui code également pour une enzyme impliquée dans la voie héparane sulfate. Des résultats préliminaires encourageants soutiennent l'hypothèse que la variation génétique de NDST1 influence la parasitémie asymptomatique. / In this thesis, we investigated the influence of some genetic variants located within chromosomes 5q31-q33, 6p21, and 17p12, which have been shown to be linked to malaria phenotypes. The genes NCR3 and TNF, which are located in the chromosomal region 6p21, have been reported to be associated with malaria in Burkina Faso population. We have replicated those studies in Congolese population to evaluate the associations of the SNPs in those genes with mild malaria attack and Plasmodium parasitemia. The results showed that the variant NCR3-412 is associated with mild malaria in Congo, and TNF-308, TNF-244, and TNF-238 are associated with mild malaria attack, maximum parasitemia, or both. In addition, bioinformatic studies suggest that TNF-244 and TNF-238 synergise to alter the binding of transcription factors.The two genes HS3ST3A1 and HS3ST3B1, which are located in chromosomal regions 17p12, are involved in the heparan sulfate proteoglycan biosynthesis. In this study, we further investigated the association of the polymorphisms in these genes with mild malaria attack and maximum parasitemia. However no association was found. We further studied the NDST1 gene, which is located within chromosome 5q31-q33, and which encodes the bifunctional enzyme N-deacetylase/ N-sulfotransferase 1, and also participates in the heparan sulfate synthesis . Encouraging results support the hypothesis that NDST1 variation influence controlling parasitemia. Further association and functional studies are needed to validate the role of NDST1 in malaria infection. More generally, the enzymes involved in the heparan sulfate pathway might play a key role in controlling malaria infection.

L’introduction de biodiversité végétale dans les agroécosystèmes peut modifier la structure des réseaux trophiques du sol et les fonctions écosystémiques qu’ils assurent. Cette thèse fixe les bases d’une démarche expérimentale pour sélectionner des espèces végétales à partir de différents traits fonctionnels et pour évaluer leurs effets sur les réseaux trophiques et le fonctionnement des sols. Nous avons plus particulièrement cherché à sélectionner des plantes de services pouvant induire la régulation des nématodes parasites du bananier Radopholus similis et Pratylenchus coffeae et favoriser la décomposition des matières organiques durant l’interculture dans les bananeraies antillaises. C'est-à-dire la période entre la destruction de la bananeraie et l'implantation d'une nouvelle. Au cours de ce travail, les réseaux trophiques du sol ont été appréhendés par une analyse fonctionnelle des communautés de nématodes. Une étude bibliographique nous a permis de montrer que les traits fonctionnels « statut d’hôtes » vis-à-vis des nématodes phytoparasites, « composition biochimique des litières » et « productivité primaire des espèces végétales » contribuent aux effets des plantes sur les réseaux trophiques du sol et sur les deux services écosystémiques étudiés. Nous avons donc effectué une typologie des plantes de services à partir de ces traits. Puis pour étudier les effets d’apports de litières de composition biochimique contrastée sur les communautés de nématodes du sol, nous avons sélectionné les espèces végétales Paspalum notatum, Crotalaria zanzibarica et Acacia auriculiformis dont les litières présentaient des teneurs variables en hémicellulose, cellulose, azote, et lignine. Une étude en microcosmes a montré que la composition biochimique des litières détermine les successions écologiques au sein des communautés de nématodes et stimule de manière contrastée les voies de décomposition de la matière organique dans les sols. Nous avons discuté des mécanismes de régulation des nématodes parasites du bananier induits par ces apports de litières. Nous avons également montré que la culture de ces trois espèces induit une diminution des populations de R. similis dans les sols. Enfin, nous avons évalué les effets au champ de couverts de P. notatum, de C. zanzibarica et d’un couvert associant ces deux espèces. Pour cela, nous avons cultivé ces couverts durant neuf mois avant de les détruire et de restituer leurs litières à la surface des sols. Nos résultats montrent que ces couverts végétaux influencent différemment l’abondance en nématodes phytoparasites et le potentiel infectieux du sol. Ils induisent également des régulations bottum-up et top down dans leurs communautés. Après la restitution des litières, nous avons observé de fortes abondances de nématodes liées à la décomposition des litières dans la couche de surface du sol. Celle-ci fournit les couches sous-jacentes du sol en azote minéral et favorise le développement des nématodes omnivores et prédateurs. Il en résulte la formation de communautés de nématodes structurées et diversifiées concomitantes à la réduction des populations de R. similis et P. coffeae. Ces résultats étaient plus marqués pour les couverts monospécifiques de C. zanzibarica et les couverts mixtes. Nos travaux confirment que les traits fonctionnels « statut d’hôte », « production primaire », « composition biochimique des litières » sont déterminant pour sélectionner des plantes de services pour l’interculture en bananeraies. / Increased plant diversity may alter soil food web structure and soil function in agroecosystems. This thesis sets the basis for an experimental approach in order to select some plant species in regard to several functional traits and to assess their effect on soil food web and soil functioning. We focused on cover-crops able to induce regulation of the banana parasitic nematodes Radopholus similis and Pratylenchus coffeae and to promote decomposition of organic matter during the fallow period in banana plantations in the French West Indies (FWI). We used functional analysis of nematode communities as a soil food web bio-indicator. Bibliography studies show us that “plant host status” with respect to plant-parasitic nematodes, “biochemical characteristics of litters” and “primary productivity of plants” are functional traits involved in plant species effects on soil food web and on the ecosystemics service we targeted. We therefore use those traits to perform a typology of cover-plants. Furthermore, to study the effects of litter inputs with contrasted biochemical characteristics we pick out the plant species Paspalum notatum, Crotalaria zanzibarica and Acacia auriculiformis which show differential hemicellulose, cellulose, lignin and nitrogen content. A microcosm assay shows that biochemical characteristics of litter determine ecological successions in nematode communities and promote, in a contrasted manner, the decomposition pathway of organic matter. We discussed the mechanisms of plant parasitic nematodes suppression involved by those litter inputs. We also confirm that the culture of those three plant species can diminish the population of R.similis in soils. We also assessed the effects of plant covers composed of P. notatum and C. zanzibarica and a mix of those two plant species on nematodes communities during a field trial. In order to do so, we cultivated those cover-plants during a nine months period and then destroyed them to restitute plant litter in soils surface. Our results showed that cover-plant alters differentially plant-parasitic nematodes and the infectious potential of soils. Cover-crop cultivation also induced bottom-up and top-down regulations in plant parasitic nematode communities. After litter restitution, we observed high abundance of nematodes involved in litter decomposition within the soil surface layer. The latter provides underlying soil layers with mineral nitrogen and improves omnivore and predator nematode abundances. It results in the formation of a structured and diversified nematode community concomitantly with R. similis and P. coffeae regulation in soils. We observed greater effects with the cover-crop composed of C. zanzibarica and the mix of C. zanzibarica and P. coffeae. This study confirms that the functional traits of “host status”, “biochemical composition of litter” and “primary production” of plant species are decisive in the selection of cover-crop for the fallow period in banana plantation.

Cette thèse est consacrée aux quelques problèmes mathématiques concernant les extensions minimales de Lipschitz. Elle est organisée de manière suivante. Le chapitre 1 est dédié à l’introduction des extensions minimales de Lipschitz. Dans le chapitre 2, nous étudions la relation entre la constante de Lipschitz d’ 1-field et la constante de Lipschitz du gradient associée à ce 1-field. Nous proposons deux formules explicites Sup-Inf, qui sont des extensions extrêmes minimales de Lipschitz d’1-field. Nous expliquons comment les utiliser pour construire les extensions minimales de Lipschitz pour les applications Rmà Rn . Par ailleurs, nous montrons que les extensions de Wells d’1- fields sont les extensions absolument minimales de Lipschitz (AMLE) lorsque le domaine d’expansion d’1-field est infini. Un contreexemple est présenté afin de montrer que ce résultat n’est pas vrai en général. Dans le chapitre 3, nous étudions la version discrète de l’existence et l’unicité de l’AMLE. Nous montrons que la fonction tight introduite par Sheffield and Smart est l’extension de Kirszbraun. Dans le cas réel, nous pouvons montrer que cette extension est unique. De plus, nous proposons un algorithme qui permet de calculer efficacement la valeur de l’extension de Kirszbraun en complexité polynomiale. Pour conclure, nous décrivons quelques pistes pour la future recherche, qui sont liées au sujet présenté dans ce manuscrit. / The thesis is concerned to some mathematical problems on minimal Lipschitz extensions. Chapter 1: We introduce some basic background about minimal Lipschitz extension (MLE) problems. Chapter 2: We study the relationship between the Lipschitz constant of 1-field and the Lipschitz constant of the gradient associated with this 1-field. We produce two Sup-Inf explicit formulas which are two extremal minimal Lipschitz extensions for 1-fields. We explain how to use the Sup-Inf explicit minimal Lipschitz extensions for 1-fields to construct minimal Lipschitz extension of mappings from Rm to Rn. Moreover, we show that Wells’s extensions of 1-fields are absolutely minimal Lipschitz extensions (AMLE) when the domain of 1-field to expand is finite. We provide a counter-example showing that this result is false in general. Chapter 3: We study the discrete version of the existence and uniqueness of AMLE. We prove that the tight function introduced by Sheffield and Smart is a Kirszbraun extension. In the realvalued case, we prove that the Kirszbraun extension is unique. Moreover, we produce a simple algorithm which calculates efficiently the value of the Kirszbraun extension in polynomial time. Chapter 4: We describe some problems for future research, which are related to the subject represented in the thesis.

Nos travaux ont un double objectif : (1) analyser conjointement les indicateurs afin de qualifier la relation entre leurs développements d’après les sollicitations fonctionnelles ; (2) étudier les modes d’exécution des activités d’un ensemble funéraire collectif vauclusien, l’hypogée des Boileau. Nous avons élargi cette étude à l’échelle départementale avec quatre gisements funéraires : les hypogées du Capitaine et de Roaix et le dolmen de l’Ubac. Le but est d’appréhender l’homogénéité ou la spécificité des comportements entre sujets et séries grâce aux différents indicateurs sur la majorité des os longs. L’étude des adaptations structurales a bénéficié de l’approche tomographique. Aucune relation directement due aux sollicitations n’a été mise en évidence entre les indicateurs.Les activités des sujets de l’hypogée des Boileau sont bilatérales et la pratique d’activités spécialisées n’a pas été mise en évidence. Les femmes ont un investissement physique similaire à celui des hommes pour le membre supérieur avec toutefois quelques différences comportementales. Les hommes sont plus mobiles et la posture récurrente du membre inférieur est différente par rapport aux femmes. La posture accroupie a été observée chez les deux sexes. Nous proposons l’existence d’une division sexuelle des tâches.La comparaison inter-échantillon indique une homogénéité comportementale avec quelques nuances selon les sites concernant principalement la posture du membre inférieur et les muscles sollicités du membre supérieur. Nos travaux apportent un nouvel éclairage sur les indicateurs de l’activité et le comportement des populations du Néolithique final. / Our work has two aims. First, the joint analysis of the indicators in order to qualify the relationship between their developments based on the mechanical loads. Then, the study of the behavioral patterns of one collective funeral deposit in Vaucluse: the hypogeum of Boileau. Then, we expand the study at the departmental scale while comparing four funeral deposits: the Capitaine and Roaix hypogeums and the dolmen of the Ubac. The goal is to apprehend the homogeneity or the specificity of behaviors between subjects and samples through the various indicators thanks to the long bones. No relationship due to mechanical loads aroused between the indicators. Their developments are not influenced by the presence of another one and do not infer it. Indicators bring complementary information on a global skeleton or a smaller scale.The activities of the Boileau hypogeum subjects are bilateral and specialized activities is difficult to prove. Males have a physical investment equal to females for the upper limb with a few behavioral differences. Men are more mobile and their recurring postural mode for the lower limb is different. The squatting posture is common for both sexes. We propose a sexual division of labor.Inter-samples stressed a behavioral uniformity with a few nuances depending of the samples along the postural mode of the lower limb and the solicited muscles of the upper limb. The terrain has no real impact on the robusticity of the lower limb for all the samples. The artifacts in the burials cannot be used to reconstruct the behavioral patterns.Our work shed light on occupational markers and the data behavioral patterns of late Neolithic populations.

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