Cyclic nucleotide-gated channels (CNGCs) are non-selective cation channels that were first identified in vertebrate photosensory and olfactory neurons. Although the physiological roles and biophysical properties of animal CNGCs have been well studied, much less is known about these channels in plants. The Arabidopsis genome encodes twenty putative CNGC subunits that are postulated to form channel complexes that mediate various physiological processes involving abiotic and biotic stress responses, ion homeostasis and development. The identification of Arabidopsis autoimmune CNGC mutants, such as defense no death class (dnd1 and dnd2), and the constitutive expressor of pathogenesis related genes 22 (cpr22) implicate AtCNGC2, 4, 11 and 12 in plant immunity. Here, I present a comprehensive study of the molecular mechanisms involved in CNGC-mediated signaling pathways with emphasis on pathogen defense. Previously, a forward genetics approach aimed to identify suppressor mutants of the rare gain-of-function autoimmune mutant, cpr22, identified key residues that are important for CNGC subunit interactions and channel function. First, I present a structure-function analysis of one of these suppressor mutants (S58) that revealed a key residue in the cyclic nucleotide binding domain involved in the stable regulation of CNGCs. Second, I present a new suppressor screen using AtCNGC2 T-DNA knockout mutants that specifically aimed to identify novel downstream components of CNGC-mediated pathogen defense signaling. In this screen, I successfully isolated and characterized the novel Arabidopsis mutant, repressor of defense no death 1 (rdd1), and expanded this study to demonstrate its involvement in AtCNGC2 and AtCNGC4-mediated signal transduction. Additionally, I demonstrated for the first time, the physical interaction of AtCNGC2 and AtCNGC4 subunits in planta. The findings presented in this thesis broaden our current knowledge of CNGCs in plants, and provide a new foundation for future elucidation of the structure-function relationships and signal transduction mediated by these channels.

Cyclic nucleotide-gated channels (CNGCs) are non-selective cation channels that were first identified in vertebrate photosensory and olfactory neurons. Although the physiological roles and biophysical properties of animal CNGCs have been well studied, much less is known about these channels in plants. The Arabidopsis genome encodes twenty putative CNGC subunits that are postulated to form channel complexes that mediate various physiological processes involving abiotic and biotic stress responses, ion homeostasis and development. The identification of Arabidopsis autoimmune CNGC mutants, such as defense no death class (dnd1 and dnd2), and the constitutive expressor of pathogenesis related genes 22 (cpr22) implicate AtCNGC2, 4, 11 and 12 in plant immunity. Here, I present a comprehensive study of the molecular mechanisms involved in CNGC-mediated signaling pathways with emphasis on pathogen defense. Previously, a forward genetics approach aimed to identify suppressor mutants of the rare gain-of-function autoimmune mutant, cpr22, identified key residues that are important for CNGC subunit interactions and channel function. First, I present a structure-function analysis of one of these suppressor mutants (S58) that revealed a key residue in the cyclic nucleotide binding domain involved in the stable regulation of CNGCs. Second, I present a new suppressor screen using AtCNGC2 T-DNA knockout mutants that specifically aimed to identify novel downstream components of CNGC-mediated pathogen defense signaling. In this screen, I successfully isolated and characterized the novel Arabidopsis mutant, repressor of defense no death 1 (rdd1), and expanded this study to demonstrate its involvement in AtCNGC2 and AtCNGC4-mediated signal transduction. Additionally, I demonstrated for the first time, the physical interaction of AtCNGC2 and AtCNGC4 subunits in planta. The findings presented in this thesis broaden our current knowledge of CNGCs in plants, and provide a new foundation for future elucidation of the structure-function relationships and signal transduction mediated by these channels.

Analyses of the evolution and function of voltage-gated sodium channel proteins (Navs) have largely been limited to mutations from individual people with diagnosed neuromuscular disease. This project investigates the carboxyl-terminus of the Nav paralog (locus scn4aa 3’) that is preferentially expressed in electric organs of Neotropical weakly-electric fishes (Order Gymnotiformes). As a model system, I used the genus Gymnotus, a diverse clade of fishes that produce species-specific electric organ discharges (EODs). I clarified evolutionary relationships among Gymnotus species using mitochondrial (cytochrome b, and 16S ribosome) and nuclear (rag2, and scn4aa) gene sequences (3739 nucleotide positions from 28 Gymnotus species). I analyzed the molecular evolution of scn4aa 3’, and detected evidence for positive selection at eight amino acid sites in seven Gymnotus lineages. These eight amino acid sites are located in motifs that may be important for modulation of EOD frequencies.

Analyses of the evolution and function of voltage-gated sodium channel proteins (Navs) have largely been limited to mutations from individual people with diagnosed neuromuscular disease. This project investigates the carboxyl-terminus of the Nav paralog (locus scn4aa 3’) that is preferentially expressed in electric organs of Neotropical weakly-electric fishes (Order Gymnotiformes). As a model system, I used the genus Gymnotus, a diverse clade of fishes that produce species-specific electric organ discharges (EODs). I clarified evolutionary relationships among Gymnotus species using mitochondrial (cytochrome b, and 16S ribosome) and nuclear (rag2, and scn4aa) gene sequences (3739 nucleotide positions from 28 Gymnotus species). I analyzed the molecular evolution of scn4aa 3’, and detected evidence for positive selection at eight amino acid sites in seven Gymnotus lineages. These eight amino acid sites are located in motifs that may be important for modulation of EOD frequencies.

Ce projet de recherche s'articule sur la validation de notre hypothèse qui affirme que l’introversion résidentielle dans la communauté métropolitaine de Montréal (CMM), ce n’est qu'une étape ultime vers la fermeture résidentielle. Ainsi, que ces lotissements prennent d’autres formes d’enclosure, qui diffèrent du modèle des Gated Communities (GCs) étatsuniennes. Ce dernier se caractérise par la clôture, la gouvernance privée, le marquage d’entrée par les portails et le flicage à travers la vidéosurveillance. Cette enclosure introvertie se réalise par la forme urbaine ̶ semi-fermée ̶ des ensembles résidentiels. Cependant, ces enclaves montréalaises se dirigent vers le modèle d’enclavement francilien; qui se caractérise par le passage doux (inaperçu) vers la fermeture résidentielle (Paquot, 2009). Précisément, nous viserons à étudier la typo-morphologie du phénomène des GCs et ses impacts par la rupture physico-spatiale, et par la ségrégation socio-spatiale sur le territoire urbain et suburbain. Notre champ d’expérimentation est la CMM. Cependant, une revue critique de la littérature sur le phénomène dans quatre territoires des pays suivants : au Canada, dans l’Ouest canadien, en France, dans l’Île-de-France, au Mexique, dans la région métropolitaine de Puebla et aux États-Unis à Los Angeles, nous a permis de tirer des conclusions en matière de définition et de divergence. La définition des GCs selon la littérature actuelle est très restreinte, elle exclut d’autres types enclos qui ne se renferment pas avec leurs clôtures, mais beaucoup plus avec leurs formes urbaines semi-fermées. Ces derniers types nous l’avions destinée à une nouvelle désignation : enclaves résidentielles introverties (ERI). Cela est après avoir réfuté la désignation d'« enclave résidentielle » utilisée par les auteurs francophones. Cette dernière n’est pas précise, elle inclut notamment tous les ensembles résidentiels qui se distinguent avec leur style individualisé par apport à leurs alentours, voire même si leur forme urbaine est ouverte. En outre, nous voulons démontrer que ces formes urbaines semi-fermées sont vraiment des lacunes au niveau des ententes conclues entre la ville (autorité locale) et les promoteurs au Québec. Ces formes urbaines sont en antinomie avec les principes du développement durable. Elles rendent la possibilité d’offrir un transport écologique impossible, leur structure est irréversible sauf si l'on va recourir à la démolition de certaines maisons pour désenclaver l’ensemble. Ces lotissements semi-fermés empêchent la circulation en éliminant la connexion de leurs rues intérieures avec le reste des voies passantes qui tissent le tissu urbain toutes autour. Parfois le panneau « cul-de-sac » garantit l’exclusivité des rues intérieures de l’ensemble résidentiel. Cette forme urbaine encourage les déplacements massifs en automobile vers les lieux de travail, étant donné qu'elle est localisée souvent loin des voies desservies par le transport en commun. En outre, cette forme semi-fermée complique l’intervention des pompiers en cas d’incendie. / This research focuses on the validation of our hypothesis which states that residential introversion in the metropolitan community of Montreal (MCM) is only the last step of residential closure. These developments are taking other forms of enclosure which differ from the design of American gated communities (GCs). These communities are characterized by the enclosure, private governance, policing through video surveillance and the marking of entry by gates. The introverted enclosure is achieved by the urban form semi-closed of residential development. However, these enclaves are like the model of closed developments in Île-de-France. This later is characterized by the uncontrolled progression to closed residential development (Paquot, 2009). Precisely, we shall study the typo-morphology of the phenomenon of GCs and their impacts of physical rupture and social-space segregation of urban and suburban territory. Our area of experimentation is the MCM, however, a criticized review of literature that analyses the phenomenon in four countries (the Canadian West; Île-de-France, France; Puebla, Mexico; and Los Angeles, United States of America) allowed us to draw conclusions regarding the divergence of definitions. The definition of GCs according to the current literature is very limited. It excludes other types of residential closing that are not characterised by their fences, but more with their semi-enclosed urban forms. So, we have refuting the label of "residential enclave" used by French authors, and then we have assigned these types a new designation: introverted residential enclaves (IRE). Our resentment is that the term of enclave residential is not precise: it includes the residential development that stands out with individual style by contributing to its surroundings, even if the urban form is open. Furthermore, we show that these semi-enclosed urban forms are indeed gaps in conventions between the local authorities and developers in Quebec. These urban forms are in contradiction with the principles of sustainable development. They imped the possibility of providing an ecological transport. Their structure is irreversible, without the demolition of certain houses in order to open up the whole. These shapes prevent also pedestrian traffic. Sometimes the panel "cul-de-sac" guarantees exclusivity of internal streets to their resident. / Plusieurs logiciels utilisés dans la réalisation des pièces graphiques incluses dans ce mémoire : ArcGIS 10; Autocad 2012; GIS Consortium de la Ville de Chicago; PowerPoint 2010, Photoshop... etc. Notre recherche est la première et la seule qui aborde le phénomène de l'introversion résidentielle au Québec. Elle se focalise sur le postulat de la représentation du phénomène de l’introversion résidentielle autant qu’une forme urbaine fermée ou semi-fermée d’une descendance accouplée aux GCs étatsuniennes. De la gated community américaine vers l’enclave francilienne, à l’introversion résidentielle montréalaise. À l'aide de notre analyse typo-morphologique, basée principalement sur la théorie de Rossi (1966), nous avons dévoilé sur un nouveau fait urbain qui existe dans la communauté métropolitaine de Montréal. Nous l'avions désigné par : enclaves résidentielles introverties (ERI).

Las enfermedades cardiovasculares (ECVs) son la principal causa de mortalidad en el mundo Occidental. El interés de prevenir y tratar las ECVs ha desencadenado un rápido desarrollo de los sistemas de adquisición de imágenes médicas. Por este motivo, la cantidad de datos de imagen recolectados en las instituciones de salud se ha incrementado considerablemente. Este hecho ha aumentado la necesidad de herramientas automatizadas para dar soporte al diagnóstico, mediante una interpretación de imagen confiable y reproducible. La tarea de interpretación requiere traducir los datos crudos de imagen en parámetros cuantitativos, los cuales son considerados relevantes para clasificar la condición cardiaca de un paciente. Para realizar tal tarea, los métodos basados en modelos estadísticos de forma han recibido favoritismo dada la naturaleza tridimensional (o 3D+t) de las imágenes cardiovasculares. Deformando el modelo estadístico de forma a la imagen de un paciente, el corazón puede analizarse de manera integral. Actualmente, el campo de las imágenes cardiovasculares esta constituido por diferentes modalidades. Cada modalidad explota diferentes fenómenos físicos, lo cual nos permite observar el órgano cardiaco desde diferentes ángulos. El personal clínico recopila todas estas piezas de información y las ensambla mentalmente en un modelo integral. Este modelo integral incluye información anatómica y funcional que muestra un cuadro completo del corazón del paciente. Es de alto interés transformar este modelo mental en un modelo computacional capaz de integrar la información de manera global. La generación de un modelo como tal no es simplemente un reto de visualización. Requiere una metodología capaz de extraer los parámetros cuantitativos relevantes basados en los mismos principios técnicos. Esto nos asegura que las mediciones se pueden comparar directamente. Tal metodología debe ser capaz de: 1) segmentar con precisión las cavidades cardiacas a partir de datos multimodales, 2) proporcionar un marco de referencia único para integrar múltiples fuentes de información, y 3) asistir la clasificación de la condición cardiaca del paciente. Esta tesis se basa en que los modelos estadísticos de forma, y en particular los Modelos Activos de Forma, son un método robusto y preciso con el potencial de incluir todos estos requerimientos. Para procesar múltiples modalidades de imagen, separamos la información estadística de forma de la información de apariencia. Obtenemos la información estadística de forma a partir de una modalidad de alta resolución y aprendemos la apariencia simulando la física de adquisición de otras modalidades. Las contribuciones de esta tesis pueden ser resumidas así: 1) un método genérico para construir automáticamente modelos de intensidad para los Modelos Activos de Forma simulando la física de adquisición de la modalidad en cuestión, 2) la primera extensión de un simulador de Resonancia Magnética Nuclear diseñado para producir estudios cardiacos realistas, y 3) un método novedoso para el entrenamiento automático de modelos de intensidad y de fiabilidad aplicado a estudios cardiacos de Resonancia Magnética Nuclear. Cada una de estas contribuciones representa un artículo publicado o enviado a una revista técnica internacional. / Cardiovascular diseases (CVDs) are the major cause of death in the Western world. The desire to prevent and treat CVDs has triggered a rapid development of medical imaging systems. As a consequence, the amount of imaging data collected in health care institutions has increased considerably. This fact has raised the need for automated analysis tools to support diagnosis with reliable and reproducible image interpretation. The interpretation task requires to translate raw imaging data into quantitative parameters, which are considered relevant to classify the patient’s cardiac condition. To achieve this task, statistical shape model approaches have found favoritism given the 3D (or 3D+t) nature of cardiovascular imaging datasets. By deforming the statistical shape model to image data from a patient, the heart can be analyzed in a more holistic way. Currently, the field of cardiovascular imaging is constituted by different modalities. Each modality exploits distinct physical phenomena, which allows us to observe the cardiac organ from different angles. Clinicians collect all these pieces of information to form an integrated mental model. The mental model includes anatomical and functional information to display a full picture of the patient’s heart. It is highly desirable to transform this mental model into a computational model able to integrate the information in a comprehensive manner. Generating such a model is not simply a visualization challenge. It requires having a methodology able to extract relevant quantitative parameters by applying the same principle. This assures that the measurements are directly comparable. Such a methodology should be able to: 1) accurately segment the cardiac cavities from multimodal datasets, 2) provide a unified frame of reference to integrate multiple information sources, and 3) aid the classification of a patient’s cardiac condition. This thesis builds upon the idea that statistical shape models, in particular Active Shape Models, are a robust and accurate approach with the potential to incorporate all these requirements. In order to handle multiple image modalities, we separate the statistical shape information from the appearance information. We obtain the statistical shape information from a high resolution modality and include the appearance information by simulating the physics of acquisition of other modalities. The contributions of this thesis can be summarized as: 1) a generic method to automatically construct intensity models for Active Shape Models based on simulating the physics of acquisition of the given imaging modality, 2) the first extension of a Magnetic Resonance Imaging (MRI) simulator tailored to produce realistic cardiac images, and 3) a novel automatic intensity model and reliability training strategy applied to cardiac MRI studies. Each of these contributions represents an article published or submitted to a peer-review archival journal.

No description available.

Submitted by PATRICIA HELENA MILANI null (patriciah.milani@gmail.com) on 2017-01-13T22:09:53Z No. of bitstreams: 1 Tese_Patrícia_Final.pdf: 5890753 bytes, checksum: 4fc6a070cf109e1149421337cb22d0ed (MD5) / Approved for entry into archive by LUIZA DE MENEZES ROMANETTO (luizamenezes@reitoria.unesp.br) on 2017-01-17T13:48:44Z (GMT) No. of bitstreams: 1 milani_ph_dr_prud.pdf: 5890753 bytes, checksum: 4fc6a070cf109e1149421337cb22d0ed (MD5) / Made available in DSpace on 2017-01-17T13:48:44Z (GMT). No. of bitstreams: 1 milani_ph_dr_prud.pdf: 5890753 bytes, checksum: 4fc6a070cf109e1149421337cb22d0ed (MD5) Previous issue date: 2016-12-07 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / La production de la différenciation socio-spatiale est analysée, en tenant compte de leurs dimensions objectives et subjectives, à partir de la comparaison entre deux villes de taille moyenne avec de différents niveaux de complexités, Catanduva et São José do Rio Preto. Le quotidien, tandis qu’une unité de l'espace et de temps est notre dimension de l’analyse, en ayant les pratiques spatiales des sujets sociaux étudiés, alors que le plan analytique, qui nous a permis d'identifier la façon dont le processus de fragmentation socio-spatiale s’exprime dans la production de l'espace urbain, surtout à partir des espaces vécus, donnant du sens et de la signification à des pratiques, qui entourent des relations contradictoires entre l'intérieur et à l'extérieur, avant et après, le changement et la permanence, l'espace et le temps. Sur la base de la réalisation de 22 entretiens et des observations sur le terrain, la recherche a révélé que, dans le discours d'une recherche de sécurité, les personnes interrogées, des habitants des espaces résidentiels fermés de classe moyenne et de l'élite, valorisent et produisent des stratégies de distinction socio-spaciale, auxquelles l'espace est dimension stratégique qui ne se limite pas au cadre d’habiter. Telle recherche modifie les façons de comme ces sujets sociaux connaissent l’urbain et ce qui lui est inhérente, étant la ville de plus en plus vécue et représentée dans les fragments. Cette tendance à la séparation, présente dans les deux villes étudiées, étend également aux pratiques de consommation, ce qui démontre de manière plus significative à São José do Rio Preto, une ville où il y a une plus grande présence de espaces fermés, d’ habitation et de consommation, largement valorisés par les segments de la classe moyenne et de l’élite. À Catanduva, il reste encore des pratiques de consommation «traditionnelles», dans le centre-ville traditionnel. Cependant, quand nous opérons à partir du processus de mise à l'échelle commune, en tenant compte les pratiques des consommations des interviewés de Catanduva qui fréquentent certains centres commerciaux de Sao Jose do Rio Preto, on vérifie que la production de la différenciation socio-spatiale est efficace, mais il exige une transposition de l'échelle intra-urbaine à être considérée dans le contexte des villes non métropolitaines. Nous concluons qu'il ne traite pas de mesurer dans quelle ville le processus se produit avec plus ou moins d'intensité, mais d'identifier les logiques qui guident les processus d'urbanisation dans la période contemporaine. / A produção da diferenciação socioespacial é analisada levando em conta suas dimensões objetivas e subjetivas, a partir da comparação entre duas cidades médias com diferentes níveis de complexidades, Catanduva e São José do Rio Preto-SP. O cotidiano, enquanto unidade de espaço e tempo, é nossa dimensão de análise, tendo as práticas espaciais dos sujeitos sociais pesquisados, enquanto plano analítico, o que nos permitiu identificar como o processo de fragmentação socioespacial se expressa na produção do espaço urbano, sobretudo a partir dos espaços vividos, conferindo sentidos e significados às práticas, que envolvem relações contraditórias entre dentro e fora, antes e depois, mudança e permanência, espaço e tempo. Com base na realização de 22 entrevistas e em observações de campo, a pesquisa revelou que, sob o discurso da busca por segurança, os sujeitos pesquisados, moradores de espaços residenciais fechados de classe média e elite, valorizam e produzem estratégias de distinção socioespacial, nas quais o espaço é dimensão estratégica que não se limita ao âmbito do morar. Tal busca modifica as maneiras como esses sujeitos sociais vivenciam o urbano e aquilo que lhe é inerente, sendo a cidade cada vez mais vivida e representada em fragmentos. Essa tendência à separação, presente nas duas cidades pesquisadas, estende-se também para as práticas de consumo, evidenciando-se de maneira mais significativa em São José do Rio Preto, cidade na qual há maior presença de espaços fechados de moradia e consumo, amplamente valorizados. Em Catanduva, ainda há permanências das práticas tradicionais de consumo, no centro principal da cidade. Porém, quando operamos a partir do processo de articulação escalar, levando em conta as práticas de consumo dos entrevistados de Catanduva que frequentam certos shopping centers de São José do Rio Preto, verifica-se que a produção da diferenciação socioespacial se efetiva, mas exige uma transposição da escala intraurbana para ser apreendido no contexto de cidades não metropolitanas, sobretudo cidades médias nos limiares. Concluímos, assim, que não se trata de medir em que cidade a diferenciação ocorre com maior ou menor intensidade, mas de identificar as lógicas que guiam os processos de urbanização no período contemporâneo. / The production of socio-spatial differentiation is analyzed in this paper considering its objective and subjective dimensions, through the comparison of two midsize cities with different complexity levels, Catanduva and São José do Rio Preto. The daily life, understood as a space-time unity, is our analysis dimension, and the spatial practices of the researched subjects are our analytical plan, which allowed us to identify how the social and spatial fragmentation process is expressed in the production of the urban space. This occurs mainly in relation to the lived spaces, which attributes meaning and significance to the practices that involve contradictory relations between the inside and the outside, the before and the after, the transformation and the continuity, space and time. We conduct our research through 22 interviews and field observations, which revealed that the researched subjects – middle class and elite residents of gate communities -, under the speech of search for security, value and produce strategies of social and spatial distinction, in which the space is a strategic dimension that is not restricted to the scope of inhabiting. This quest modifies the ways these social subjects experience the urbane, with all the things that are connected to it, and in this manner the city is progressively lived and represented in fragments. This tendency to separation, observed in the two researched cities, also reaches the purchasing practices, despite being more significant in São José do Rio Preto, because there are more gated spaces of residence and purchase in this city. In Catanduva, there still are traditional practices of purchasing in the center of the city. However, when we operate in the scale articulation process, considering the purchasing practices of the interviewed subjects that live in Catanduva but attend the shopping centers of São José do Rio Preto, we observe the existence of the differentiation process as well. The difference is that this process requires a transposition of the intra-urban scale in order to be apprehended in the context of non-metropolitan cities, mainly in the case of threshold midsize cities. Our conclusion is that we should not consider the size of the city in order to analyze the process, but to identify the logics that drive the urbanization processes in the contemporary period.

Na prática intervencionista, a ultrassonografia intravascular (USIV) é usada para se obter informações quantitativas e qualitativas do acometimento aterosclerótico, de forma complementar à angiografia. Esta tese teve como objetivos explorar a característica tomográfica do exame de USIV, bem como sua dinâmica dentro do ciclo cardíaco. Para isso, desenvolvemos técnicas de processamento de imagens médicas. Primeiramente, investigamos a reconstrução tridimensional da coronária baseando-nos apenas nas imagens de USIV, ou seja, sem a angiografia, como é feita a reconstrução atualmente. Na análise da dinâmica, fizemos um estudo para dispor volumes da coronária em diferentes fases do ciclo cardíaco de forma que estivessem alinhados espacialmente. Como consequência dos tratamentos propostos anteriormente, realizamos estudos sobre a quantificação de propriedades mecânicas dentro das condições oferecidas no intervalo de um ciclo cardíaco. As metodologias propostas foram aplicadas em simulações numéricas desenvolvidas neste trabalho e em exames reais. Obtivemos resultados compatíveis com os objetivos iniciais para reconstrução tridimensional da USIV em simulações numéricas. Na análise da dinâmica, a reconstrução de volumes em diferentes fases do ciclo e o alinhamento espacial possibilitaram a quantificação da variação setorial de volume da luz do vaso durante o ciclo cardíaco / In percutaneous coronary interventions, intravascular ultrasound (IVUS) examination is used to retrieve quantitative and qualitative information about the atherosclerotic plaque progression, complementary to angiography examination. This thesis has as objectives to explore the tomographic characteristic of the IVUS examination, as well as its dynamics within a cardiac cycle. For that purpose, medical image processing techniques were developed. Firstly, we have investigated how to reconstruct the tridimensional coronary based only on IVUS images, that is, without angiography, as it is done nowadays. Regarding dynamic analysis, we have studied models to build volumes of the coronary in distinct phases of the cardiac cycle in a spatial aligned way. Conversantly, as a consequence of the previous image processing methods, we have studied the quantification of mechanical properties of the vessel wall within a cardiac cycle. The methodologies proposed were applied in numeric phantoms developed in this work and also in real IVUS examinations. As result, tridimensional reconstruction was successful in the numeric phantom approach. In dynamics analysis, the reconstruction in distinct cardiac phases and volumes spatial alignment enabled the quantification of lumen volume variation during the cardiac cycle

Over the last few years the city of Anaheim has undertaken several significant redevelopment projects designed to revitalize some of the older, more run down areas of the city. One of these projects was the redevelopment of the Jeffrey-Lynne neighborhood, an area that had been plagued by crime. The redevelopment involved the complete remodeling of the existing housing structure into lower density housing within a gated community. This study examines the impact of the redevelopment on the crime rate in this neighborhood; it employs location quotient analyses for six geographic levels on four crime categories (property, violence, disorder, drug) and five crime types (disturbance, robbery, burglary, assault, auto theft). The results reveal that the effects of the redevelopment on the crime rate were mixed.