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Peptídeos sintéticos no estudo do sistema toxina-antitoxina ParE/ParD / Synthetic peptides in the study of the toxin-antitoxine system ParE/ParDBenites, Thais Azevedo [UNESP] 27 June 2017 (has links)
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Previous issue date: 2017-06-27 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O sistema ParE-ParD é um sistema Toxina-Antitoxina (TA) do tipo II (composto por duas proteínas) encontrado no plasmídeo RK2 de uma gama de bactérias. A antitoxina ParD (9kDa) é capaz de neutralizar a citotoxicidade da toxina ParE, pela formação de um complexo estável, e também é eficaz na auto-repressão do operon parDE. A toxina (12kDa) apresenta atividade citotóxica no processo de replicação do DNA por interferir diretamente na ação da DNA girase. Estudos prévios sugeriram que a região C-terminal da antitoxina é responsável pelo processo de interação com ParE. Embora esta toxina possa ser encontrada em um grande número de microrganismos, ainda apresenta mecanismos de citotoxicidade e funções celulares a serem elucidadas. Neste contexto, este trabalho teve como objetivo a tentativa de expressão das duas proteínas ParE e ParD, bem como o design e a síntese de peptídeos análogos da antitoxina, para a realização de estudos de interação molecular, a fim de encontrar uma estrutura mínima de ParD capaz de inativar a função toxica de ParE. Com base nas informações estruturais, obtidas por modelagem e dinâmica molecular, quatro sequências peptídicas análogas de ParD foram projetadas e sintetizadas pela metodologia da fase sólida. As sequências foram analisadas e purificadas por cromatografia líquida de alta eficiência e caracterizadas por espectrometria de massas. Os estudos de interação foram realizados através de ensaios de cromatografia de afinidade e supressão de fluorescência. A fluorescência intrínseca de ParEAC2 foi suprimida pelos análogos de ParD (ParDTB1, ParDTB3, ParDTB5 e ParDTB6), evidenciando a formação de complexos estáveis entre as espécies, resultados confirmados pelos ensaios de cromatografia de afinidade. Resultados semelhantes foram obtidos empregando a proteína ParD obtida por expressão heteróloga. Com base nos resultados obtidos, foi possível concluir que o análogo ParDTB1 representa uma estrutura peptídica mínima com potencial para neutralizar o efeito da toxina ParE. / The ParE-ParD system is a toxin-antitoxin (TA) type II module (composed of two proteins) of the plasmid RK2 of a range of bacteria. The ParD antitoxin (9 kDa) is able to neutralize the cytotoxicity of the ParE toxin by forming a stable complex and is effective in the auto repression of the parDE operon. The toxin (12 kDa) exhibits cytotoxic activity by blocking DNA replication, acting directly in the DNA gyrase action. Previous studies have been suggest that the C-terminal region of the antitoxin is responsible for the interaction process with ParE. Although this toxin can be find in a large number of microorganisms, still have cytotoxicity mechanisms and cellular functions to be elucidate. In this context, this work aimed at the expression of ParE and ParD proteins, as well as the design and synthesis of antitoxin analog peptides, to perform molecular interaction studies in order to find a minimum ParD structure able to inactivate the toxic function of ParE. Based on the structural information obtained by modeling and molecular dynamics, four analogous peptide sequences of ParD were designed and synthesized by the solid phase methodology. The peptide sequences were analyzed and purified by high performance liquid chromatography and characterized by mass spectrometry. Interaction studies were performed by affinity chromatography and fluorescence suppression assays. The intrinsic fluorescence of ParEAC2 was suppressed by ParD analogs (ParDTB1, ParDTB3, ParDTB5 and ParDTB6) addition, evidencing the formation of stable complexes between the species, results confirmed by the affinity chromatography assays. Similar results were obtained using ParD protein obtained by heterologous expression. Based on the results obtained, it was possible to conclude that the ParDTB1 analog represents a minimal peptide structure with potential to neutralize the effect of the ParE toxin.
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Analyse de la corrélation conditionnelle dérivée de la coévolution d’un système de trois gènes par un modèle du maximum de vraisemblanceBenoit Bouvrette, Louis Philip 08 1900 (has links)
Les gènes codant pour des protéines peuvent souvent être regroupés et intégrés en modules fonctionnels par rapport à un organelle. Ces modules peuvent avoir des composantes qui suivent une évolution corrélée pouvant être conditionnelle à un phénotype donné. Les gènes liés à la motilité possèdent cette caractéristique, car ils se suivent en cascade en réponse à des stimuli extérieurs. L’hyperthermophilie, d’autre part, est interreliée à la reverse gyrase, cependant aucun autre élément qui pourrait y être associé avec
certitude n’est connu. Ceci peut être dû à un déplacement de gènes non orthologues encore non résolu. En utilisant une approche bio-informatique, une modélisation mathématique d’évolution conditionnelle corrélée pour trois gènes a été développée et appliquée sur des profils phylétiques d’archaea. Ceci a permis d’établir des théories quant à la fonction potentielle du gène du flagelle FlaD/E ainsi que l’histoire évolutive des gènes lui étant liés et ayant contribué à sa formation. De plus, une histoire évolutive théorique a été établie pour une ligase liée à l’hyperthermophilie. / Protein coding gene may often be grouped and integrated in functional modules with respect to an organelle. These modules may have constituents that follow a conditional correlated evolution to a given phenotype. Genes linked to motility posses this characteristic as they follow a cascade in response to external stimuli. Similarly, hyperthermophily is related to reverse gyrase, however no other element that could be associated with certainty is known. This may be caused by an unresolved case of non-orthologous gene displacement. Using a bioinformatic approach, a mathematical model for conditional correlated evolution for three genes has been developed and applied to the phyletic
profiles of archaea. This has helped to develop theories about the potential functions of the flagellar gene FlaD/E and the evolutionary history of the genes that are linked to it and that may have contributed to its formation. In addition, a theoretical evolutionary history has been established for a ligase associated with hyperthermophily.
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Analyse de la corrélation conditionnelle dérivée de la coévolution d’un système de trois gènes par un modèle du maximum de vraisemblanceBenoit Bouvrette, Louis Philip 08 1900 (has links)
Les gènes codant pour des protéines peuvent souvent être regroupés et intégrés en modules fonctionnels par rapport à un organelle. Ces modules peuvent avoir des composantes qui suivent une évolution corrélée pouvant être conditionnelle à un phénotype donné. Les gènes liés à la motilité possèdent cette caractéristique, car ils se suivent en cascade en réponse à des stimuli extérieurs. L’hyperthermophilie, d’autre part, est interreliée à la reverse gyrase, cependant aucun autre élément qui pourrait y être associé avec
certitude n’est connu. Ceci peut être dû à un déplacement de gènes non orthologues encore non résolu. En utilisant une approche bio-informatique, une modélisation mathématique d’évolution conditionnelle corrélée pour trois gènes a été développée et appliquée sur des profils phylétiques d’archaea. Ceci a permis d’établir des théories quant à la fonction potentielle du gène du flagelle FlaD/E ainsi que l’histoire évolutive des gènes lui étant liés et ayant contribué à sa formation. De plus, une histoire évolutive théorique a été établie pour une ligase liée à l’hyperthermophilie. / Protein coding gene may often be grouped and integrated in functional modules with respect to an organelle. These modules may have constituents that follow a conditional correlated evolution to a given phenotype. Genes linked to motility posses this characteristic as they follow a cascade in response to external stimuli. Similarly, hyperthermophily is related to reverse gyrase, however no other element that could be associated with certainty is known. This may be caused by an unresolved case of non-orthologous gene displacement. Using a bioinformatic approach, a mathematical model for conditional correlated evolution for three genes has been developed and applied to the phyletic
profiles of archaea. This has helped to develop theories about the potential functions of the flagellar gene FlaD/E and the evolutionary history of the genes that are linked to it and that may have contributed to its formation. In addition, a theoretical evolutionary history has been established for a ligase associated with hyperthermophily.
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Mechanisms of resistance to ciprofloxacin in Neisseria gonorrhoeae /Lindbäck, Emma, January 2006 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2006. / Härtill 5 uppsatser.
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Thermus thermophilus Argonaute Functions in the Completion of DNA ReplicationJolly, Samson M. 20 May 2020 (has links)
Argonautes (AGOs) are present in all domains of life. Like their eukaryotic counterparts, archaeal and eubacterial AGOs adopt a similar global architecture and bind small nucleic acids. In many eukaryotes, AGOs, guided by short RNA sequences, defend cells against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. We find that TtAgo also participates in DNA replication. In vivo, TtAgo binds 15–18 nt DNA guides derived from the chromosomal region where replication terminates, and TtAgo complexed to short DNA guides enhances target finding and prefers to bind targets with full complementarity. Additionally, TtAgo associates with proteins known to act in DNA replication. When gyrase, the sole T. thermophilus type II topoisomerase, is inhibited, TtAgo allows the bacterium to finish replicating its circular genome. In contrast, loss of both gyrase and TtAgo activity slows growth and produces long, segmented filaments in which the individual bacteria are linked by DNA. Furthermore, wild-type T. thermophilus outcompetes an otherwise isogenic strain lacking TtAgo. Finally, at physiologic temperature in vitro, we find TtAgo possesses highest affinity for fully complementary targets. We propose that terminus-derived guides binding in such a fashion localize TtAgo, and that the primary role of TtAgo is to help T. thermophilus disentangle the catenated circular chromosomes generated by DNA replication.
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Biased Evolution : Causes and ConsequencesBrandis, Gerrit January 2016 (has links)
In evolution alternative genetic trajectories can potentially lead to similar phenotypic outcomes. However, certain trajectories are preferred over others. These preferences bias the genomes of living organisms and the underlying processes can be observed in ongoing evolution. We have studied a variety of biases that can be found in bacterial chromosomes and determined the selective causes and functional consequences for the cell. We have quantified codon usage bias in highly expressed genes and shown that it is selected to optimise translational speed. We further demonstrated that the resulting differences in decoding speed can be used to regulate gene expression, and that the use of ‘non-optimal’ codons can be detrimental to reading frame maintenance. Biased gene location on the chromosome favours recombination between genes within gene families and leads to co-evolution. We have shown that such recombinational events can protect these gene families from inactivation by mobile genetic elements, and that chromosome organization can be selectively maintained because inversions can lead to the formation of unstable hybrid operons. We have used the development of antibiotic resistance to study how different bacterial lifestyles influence evolutionary trajectories. For this we used two distinct pairs of antibiotics and disease-causing bacteria, namely (i) Mycobacterium tuberculosis that is treated with rifampicin and (ii) Escherichia coli that is treated with ciprofloxacin. We have shown that in the slow-growing Mycobacterium tuberculosis, resistance mutations are selected for high-level resistance. Fitness is initially less important, and over time fitness costs can be ameliorated by compensatory mutations. The need for rapid growth causes the selection of ciprofloxacin resistance in Escherichia coli not only to be selected on the basis of high-level resistance but also on high fitness. Compensatory evolution is therefore not required and is not observed. Taken together, our results show that the evolution of a phenotype is the product of multiple steps and that many factors influence which trajectory is the most likely to occur and be most beneficial. Over time, selection will favour this particular trajectory and lead to biased evolution, affecting genome sequence and organization.
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Towards time-resolved cryo-EM of SARS-CoV-2 replication-transcription complex and Staphylococcus aureus DNA gyraseKrálová, Anna January 2023 (has links)
Time-resolved cryo-EM has already provided ground-breaking discoveries in various fields, including structural biology, biochemistry, and drug development. Compared to traditional structural biology methods where mostly stabilized conformations are reconstructed, the main advantage of time-resolved cryo-EM is its ability to capture dynamic processes in biological samples at near-atomic resolution, which allows for studying biological structures as they change and interact in real-time. In this project, I focused on the expression and purification of the individual proteins of two dynamic molecular complexes – Staphylococcus aureus (S. aureus) DNA gyrase and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) replication-transcription complex – and attempted to assemble them into their functional forms for cryo-EM imaging. Both of these complexes are interesting drug targets as they play an essential role in nucleic acid replication. The function of DNA gyrase is to modulate DNA supercoiling, facilitate DNA replication, and resolve intertwined DNA molecules. The replication-transcription complex of SARS-CoV-2 comprises, among other proteins, the RNA-dependent RNA polymerase, which, together with non-structural proteins 7 and 8, is responsible for the replication of the viral genome. There are still many questions about the underlying mechanisms of these key processes, and time-resolved cryo-EM studies will provide valuable information to advance our understanding of them. Here I present expression and purification protocols for S. aureus DNA gyrase subunits A and B and SARS-CoV-2 non-structural proteins 7, 8 and 12. DNA gyrase subunits A and B were expressed in Escherichia coli (E. coli) and purified in several steps, including affinity chromatography (His-Trap), ion exchange chromatography (IEX) and size exclusion chromatography (SEC). Despite many challenges with gyrase A precipitation, I obtained enough of both subunits for the intended cryo-EM. Different strategies to assemble them into a functional tetramer were tested but did not result in the expected outcome. The gained knowledge about the behaviour of the subunits in solution will serve as a basis for further optimization of the protocols before the assembly of the complex can be attempted again. Non-structural proteins 7 and 8 were expressed in E. coli as a polyprotein and successfully purified using His-Trap and SEC. I obtained a great amount of the polyprotein and established a protocol for its cleavage. Nsp12 was expressed using the baculovirus-insect cell expression system. The immunofluorescence assay data showed that the tested lipofection protocol works, and nsp12 is being produced in sufficient quantities. This result provides a solid base for further experiments to establish a purification method and assemble the nsp12-nsp7-nsp8 complex for cryo-EM imaging.
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Rôle de la topoisomérase I durant la transcription chez Escherichia coliMassé, Éric 12 1900 (has links)
Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal. / Les ARN produits chez la bactérie Escherichia coli sont généralement amenés vers deux voies distinctes. Le plus souvent, l' ARN naissant se dissocie de la matrice, durant sa synthèse, afin d'être fonctionnel. Celui-ci peut alors servir de matrice pour les ribosomes qui le traduisent en polypeptides, ou alors il peut servir de matériau de structure dans la composition des ribosomes. Le second type de transcrit trouve son utilité lorsque l' ARN demeure attaché à la matrice ADN afin d'amorcer la réplication. Ce type d' ARN est très court et il est immédiatement utilisé comme amorce par la polymérase ADN de la cellule. Récemment, il a été démontré que les hybrides ARNADN peuvent aussi être impliqués dans la recombinaison. Plusieurs travaux menés sur la formation de ces structures hybrides indiquent que celle-ci dépend généralement du surenroulement de l' ADN et de certains paramètres de transcription.
La formation d'hybrides ARN-ADN durant la transcription démontre bien que la compréhension des mécanismes de transcription serait évidemment incomplète sans y inclure la topologie de l' ADN. L'étude de la transcription révèle un mécanisme dynamique très complexe, finement régulé à plusieurs niveaux, tant à l'initiation, l'élongation, que la terminaison. La topologie de l' ADN, ainsi que les topoisomérases ADN ont été caractérisées comme des modulateurs importants dans toutes les étapes de l'expression génique, allant de la reconnaissance spécifique des promoteurs jusqu'au déplacement de l' ARN naissant ou de son attachement à la matrice ADN. De plus, la transcription est l'évènement génétique le plus fréquent dans une cellule.
Selon le travail présenté ici, un des rôles essentiels de la topoisomérase I semble être l'inhibition de la formation d'hybrides ARN-ADN durant la transcription chez Escherichia coli. Ceux-ci semblent survenir principalement au niveau des opérons ribosomaux. Le couplage entre la traduction et la transcription, chez les ARNm, aide à prévenir la formation d'hybrides ARN-ADN. La croissance à basse température favorise l'appariement de l' ARN à l' ADN. La suite des travaux décrits propose que le rôle essentiel de la topoisomérase I chez Escherichia coli n'est pas la relaxation du surenroulement global de l' ADN, mais plutôt l'élimination du surenroulement local généré durant la transcription. Ce surenroulement semble responsable de l'initiation de la formation de structures hybrides ARN-ADN, alors que la gyrase provoquerait leur élongation. Ces observations permettent de suggérer de nouveaux rôles pour la topoisomérase I et la gyrase chez les bactéries.
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Probing bacterial uptake of glycosylated ciprofloxacin conjugatesMilner, S.J., Carrick, C. ., Kerr, Kevin G., Snelling, Anna M., Thomas, G.H., Duhme-Klair, A-K., Routledge, A. January 2014 (has links)
No / Mono- and disaccharide-functionalised conjugates of the fluoroquinolone antibiotic ciprofloxacin have been synthesised and used as chemical probes of the bacterial uptake of glycosylated ciprofloxacin. Their antimicrobial activities against a panel of clinically relevant bacteria were determined: the ability of these conjugates to inhibit their target DNA gyrase and to be transported into the bacteria was assessed by using in vivo and in vitro assays. The data suggest a lack of active uptake through sugar transporters and that although the addition of monosaccharides is compatible with the inhibition of DNA gyrase, the addition of a disaccharide results in a significant decrease in antimicrobial activity.
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Rôle des topoisomérases de type IA dans la ségrégation des chromosomes chez Escherichia coliTanguay, Cynthia 12 1900 (has links)
Les topoisomérases I (topA) et III (topB) sont les deux topoisomérases (topos) de type IA d’Escherichia coli. La fonction principale de la topo I est la relaxation de l’excès de surenroulement négatif, tandis que peu d’information est disponible sur le rôle de la topo III. Les cellules pour lesquelles les deux topoisomérases de type IA sont manquantes souffrent d’une croissance difficile ainsi que de défauts de ségrégation sévères. Nous démontrons que ces problèmes sont majoritairement attribuables à des mutations dans la gyrase qui empêchent l’accumulation d’excès de surenroulement négatif chez les mutants sans topA. L’augmentation de l’activité de la gyrase réalisée par le remplacement de l’allèle gyrB(Ts) par le gène de type sauvage ou par l’exposition des souches gyrB(Ts) à une température permissive, permet la correction significative de la croissance et de la ségrégation des cellules topos de type IA. Nous démontrons également que les mutants topB sont hypersensibles à l’inhibition de la gyrase par la novobiocine. La réplication non-régulée en l’absence de topA et de rnhA (RNase HI) augmente la nécessité de l’activité de la topoisomérase III. De plus, en l’absence de topA et de rnhA, la surproduction de la topoisomérase III permet de réduire la dégradation importante d’ADN qui est observée en l’absence de recA (RecA). Nous proposons un rôle pour la topoisomérase III dans la ségrégation des chromosomes lorsque l’activité de la gyrase n’est pas optimale, par la réduction des collisions fourches de réplication s’observant particulièrement en l’absence de la topo I et de la RNase HI. / E. coli possesses two type IA topoisomerases (topos), namely topo I (topA) and topo III (topB). The major function of topo I is the relaxation of excess negative supercoiling. Much less is known about the function of topo III. Cells lacking both type IA topos suffer from severe chromosome segregation and growth defects. We show that these defects are mostly related to the presence of gyrase mutations that prevent excess negative supercoiling in topA null mutants. Indeed, increasing gyrase activity by spontaneous mutations, by substituting a gyrB(Ts) allele for a wild-type one or by exposing cells carrying the gyrB(Ts) allele to permissive temperatures, significantly corrected the growth and segregation defects of cells lacking type IA topo activity. We also found that topB mutants are hypersensitive to novobiocin due to gyrase inhibition. Our data also suggest that unregulated replication occurring in the absence of topA and rnhA (RNase HI) exacerbates the need for topo III activity. Moreover, when topA and rnhA were absent, we found that topo III overproduction reduced the extensive DNA degradation that took place in the absence of recA (RecA). All together, our results lead us to propose a role for topo III in chromosome segregation when gyrase activity is suboptimal, thus reducing replication forks collapse, especially when replication is unregulated due to the absence of topo I and RNase HI.
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