Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-07-11T17:13:30Z No. of bitstreams: 1 MaraLuanaBatistaSevero_DISSERT.pdf: 1279812 bytes, checksum: d55a74719b46a51718b7399d61a2a556 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-07-15T18:45:17Z (GMT) No. of bitstreams: 1 MaraLuanaBatistaSevero_DISSERT.pdf: 1279812 bytes, checksum: d55a74719b46a51718b7399d61a2a556 (MD5) / Made available in DSpace on 2016-07-15T18:45:17Z (GMT). No. of bitstreams: 1 MaraLuanaBatistaSevero_DISSERT.pdf: 1279812 bytes, checksum: d55a74719b46a51718b7399d61a2a556 (MD5) Previous issue date: 2016-02-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Objetivo desse estudo foi analisar a imunoexpress?o de receptores de calcitonina (CTRs) e glicocorticoides (GCRs) em les?es centrais de c?lulas gigantes (LCCGs) agressivas e n?o agressivas. Trata-se de um estudo imuno-histoqu?mico (t?cnica da imunoperoxidase), quantitativo e descritivo de 52 casos de LCCGs dos ossos maxilares, nos quais 13 pacientes portadores de LCCG foram tratados com triancinolona intralesional ou calcitonina spray intranasal. A m?dia de imunomarca??o foi comparada entre os tipos celulares e subtipo cl?nico da les?o. O teste de Mann-Whitney foi realizado para essas compara??es. Dos 52 casos de LCCGs, 53.8% eram do g?nero feminino, com uma m?dia de idade de 25.69 anos. A mand?bula foi o s?tio anat?mico mais acometido. Trinta casos (57.7%) foram de LCCGs agressivas e 22 (42.3%) de n?o agressivas. A cirurgia foi o tratamento de escolha em 75% das LCCGs estudadas. Em 56.7% das LCCGs agressivas foi realizada cirurgia, enquanto 43.4% foram submetidas ao tratamento conservador. Dos submetidos ao tratamento conservador, a maioria (n = 8; 61.5%) respondeu bem ao tratamento. A express?o de CTR foi evidenciada em 67.3% da amostra e para o GCR em 96.15% dos casos. N?o houve diferen?a estatisticamente significante entre a express?o de CTRs e GCRs em c?lulas mononucleares e multinucleadas das LCCGs em rela??o ? agressividade, em rela??o ao tratamento realizado para os casos de les?es agressivas e em rela??o ? resposta ao tratamento conservador realizado nos casos de LCCGs agressivas (p>0.05). Os resultados da nossa pesquisa sugerem que a imunoexpress?o dos CTRs e GCRs n?o influenciou na resposta ao tratamento cl?nico com calcitonina ou triancinolona na amostra estudada e exibiu uma express?o variada independente da agressividade da les?o. / The aim of this study was to analyze the immunoexpression of calcitonin (CTR) and glucorticoid (GCR) receptors in aggressive and non-aggressive central giant cell lesions (CGCL). This is an immunohistochemistry study (immunoperoxidase technique) of 52 cases of CGCL of the jaws, in which 12 patients were treated with intralesional triamcinolone injections and one with calcitonin nasal spray. The mean of immunostaining was compared between the cell types and clinical subtype of the lesion. The correlations among means were analyzed by Mann-Whitney test. Of the 52 cases studied, 53.8% were females, with a mean of 25.69 years. Most lesions were located in the mandible. Thirty patients (57.7%) had aggressive lesions and 22 (42.3%) of the cases consisted of non-aggressive lesions. Surgery was the treatment of choice in 75% of the cases. In 56.7% of the aggressive CGCL surgery was performed, while 43.4% of patients were submitted to conservative treatment. Among cases submitted to conservative treatment, the majority (n = 8; 61.5%) responded well to treatment. CTR expression was observed in 67.3% and GCR in 96.15% of cases. There was no significant statistical difference between the expression of CTRs and GCRs in mononuclear and multinucleated CGCLscells, regarding aggressiveness, treatment performed for aggressive lesions and the response to conservative treatment (p>0.05). The results of our research suggest that the immunoreactivity of CTRs and GCRs did not influence the response to clinical treatment with calcitonin or triamcinolone in the sample studied and it exhibited a varied expression regardless of the aggressiveness of the lesion.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-07-25T23:14:34Z No. of bitstreams: 1 AngelicaLopesCordeiroMandu_DISSERT.pdf: 8297335 bytes, checksum: a571100e33585465a7dd9329c7047ab6 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-08-04T20:58:54Z (GMT) No. of bitstreams: 1 AngelicaLopesCordeiroMandu_DISSERT.pdf: 8297335 bytes, checksum: a571100e33585465a7dd9329c7047ab6 (MD5) / Made available in DSpace on 2016-08-04T20:58:54Z (GMT). No. of bitstreams: 1 AngelicaLopesCordeiroMandu_DISSERT.pdf: 8297335 bytes, checksum: a571100e33585465a7dd9329c7047ab6 (MD5) Previous issue date: 2016-02-18 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Os nevos melanoc?ticos (NMs) s?o prolifera??es benignas de c?lulas n?vicas, que podem serencontradas na pele e em mucosas de revestimento, incluindo a mucosa oral. Contudo, osNMs cut?neos s?o mais comuns, quando comparados os que acometem a mucosa oral. Osmecanismos moleculares envolvidos no desenvolvimento dos nevos e os fatores que podeminfluenciar no padr?o de migra??o das c?lulas n?vicas s?o pouco explorados. O objetivo destapesquisa foi analisar a express?o imuno-histoqu?mica das prote?nas E-caderina e Bcl-2 emNMs orais/cut?neos e relacion?-las com as caracter?sticas cl?nicas (sexo, idade, localiza??o,exposi??o ? radia??o solar) e tipos histopatol?gicos. Foram analisados 36 casos de NMs orais34 de NMs cut?neos. Foi utilizada a t?cnina de imuno-histoqu?mica das prote?nas E-caderinae bcl-2, na qual foram analisados a intensidade (fraca, intermedi?ria e forte) e distribui??o demarca??o (focal e difusa). A imunoexpress?o tamb?m foi analisada quanto aos tipos de c?lulasn?vicas (A, B e C). A an?lise estat?stica foi realizada atrav?s dos testes de Qui-Quadradode Pearson e Correla??o de Spearman com n?vel de signific?ncia estabelecido em 5%. Dos 70casos de NMs, 82,9% eram do sexo feminino, 48,6% com idade entre 26-50 anos, 60% eramda ra?a branca, 51,4% foram diagnosticados histopatologicamente como nevos intrad?rmicos/intramucosos e 80% eram NMs adquiridos. A express?o imuno-histoqu?mica da bcl2 e Ecaderinaforam vari?veis na amostra e n?o exibiram associa??o com os par?metros cl?nicos. Aexpress?o da bcl-2 e E-caderina foram vari?veis de acordo com os tipos de c?lulas n?vicas (A,B e C) (p=0,001). A express?o da bcl-2 foi mais difusa em NMs cong?nitos (p=0,002). A Ecaderinafoi positiva em 83,3% dos NMs <1cm (p=0,001) e em exibiu uma fraca marca??oem 73,9% dos NMs que se encontravam em ?reas expostas (p=0,010). Com base nestes resultados,sugere-se que a E-caderina tenha um controle na determina??o dos tipos histopatol?gicosdos NMs, e que a bcl-2 seja um poss?vel marcador de NMs com maior susceptibilidade aodesenvolvimento de les?es malignas. / Melanocytic nevi (MNs) are benign melanocytic proliferations of cells, which can be found in the skin and mucous coat, including the oral mucosa. However, skin NMs are more common when compared to those that affect the oral mucosa. The molecular mechanisms involved in the development of nevi and the factors that can influence the migration pattern of the nevus cells are little explored. The aim of this study was to analyze the immunohistochemical expression of E-cadherin protein and Bcl-2 in oral / skin NMs and relate them to the clinical characteristics (gender, age, location, exposure to solar radiation) and histopathological types. 36 cases of oral NMs and 34 Skin NMs were analyzed. The immunohistochemistry was used of the protein E-cadherin and bcl-2, which were analyzed the intensity (weak, moderate and strong) and distribution marking (diffuse and focal). The immunoreactivity also analyzed as to the types of nevus cells (epithelioid cells -A, -B lymphocyte and fibroblast-like -C). Statistical analysis was performed using the chi-square tests of Pearson and Spearman correlation with significance level set at 5%. Of the 70 cases of NMs, 82.9% were female, 48.6% aged 26-50 years, 51.4% were diagnosed histologically as intradermal / intramucosal nevi and 80% were NMs acquired. Immunohistochemical expression of BCL2 and E-cadherin were variables in the sample and showed no association with clinical parameters. The expression of bcl-2 and E-cadherin were variable according to the types of nevus cells (A, B and C) (P = 0.001). The expression of bcl-2 was more diffuse in congenital MNs (p = 0.002). E-cadherin was positive in 83.3% of MNs <1cm (p = 0.001) and exhibited weak staining in 73.9% of MNs that were in exposed areas (p = 0.010). Based on these results, it is suggested that the E-cadherin has a modulating effect on the migratory properties of NMs, and bcl-2 is a marker of MNs with increased proliferative capacity.

Made available in DSpace on 2014-12-17T15:32:28Z (GMT). No. of bitstreams: 1 PollianaMA.pdf: 3849977 bytes, checksum: 1bd9caa4d33368bb137c3e70ddabe0df (MD5) Previous issue date: 2009-03-06 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Several studies are carried out with aim to establish parameters to determine biologic behavior of oral squamous cell carcinoma, in order this neoplasm presents high rates of morbidity and mortality. The purpose of present research was to performe a clinic, morphologic and immunohistochemical analysis by the expression of galectins 1, 3, 4 and 7 in 65 cases of tongue squamous cell carcinoma, correlating this expression with clinics (outcome of the disease, metastasis and clinical staging) and morphologic parameters (malignancy histologic gradation system). The clinical and morphologic parameters analysed and expression of galectins 1, 3, 4 and 7 were submitted to statistical analysis (Qui2 test), observing that can be utilized as indicators of the biological behavior of the tongue squamous cell carcinoma. The galectin 1 was expressed in 87,7% of cases studied and it exhibit statistically significant correlation with metastasis (p=0,033) and clinical staging (p=0,016), it is located mostly in the citoplasm of the stomal cells. The immunoexpression of galectin 3 in 87,7% of cases was correlated with the presence of metastasis (p=0,033) and malignancy histological gradation system (p=0,031), observed, mostly of cases, in tongue squamous cell carcinoma of malignancy high grading. The galectin 4 showed no statistical significance to any of the parameters evaluated. The expression of galectin 7 in 73,8% of cases showed statistically significant correlation with the malignancy histologic grading (p=0,005), which is marking exclusively found in neoplastic epithelial cells, in the mostly of cases, it is found in cytoplasm and membrane (50%). The expressive immunopositivy of the galectins 1, 3 and 7, observed in this research, leads us to suggest a broad participation of these proteins in oral carcinogenesis, and its possible use as markers of biological behavior and tumor progression in cases of squamous cell carcinoma of the tongue / Diversos estudos s?o desenvolvidos com o intuito de se estabelecer par?metros para determinar o comportamento biol?gico do carcinoma epiderm?ide oral, tendo em vista que esta neoplasia apresenta altas taxas de morbidade e mortalidade. O objetivo do presente trabalho foi realizar uma an?lise cl?nica, morfol?gica e imuno-histoqu?mica atrav?s da express?o das galectinas 1, 3, 4 e 7 em 65 casos de carcinoma epiderm?ide de l?ngua, correlacionando essa express?o ? par?metros cl?nicos (desfecho da doen?a, met?stase, estadiamento cl?nico) e morfol?gicos (sistema de grada??o histol?gica de malignidade). Os par?metros cl?nicos e morfol?gicos analisados e a express?o das galectinas 1, 3, 4 e 7 foram submetidos a an?lise estat?stica (teste do Qui 2), observando-se que os mesmos podem ser utilizados como indicadores do comportamento biol?gico do carcinoma epiderm?ide de l?ngua. A galectina 1 foi expressa em 87,7% dos casos, apresentando correla??o estatisticamente significativa com a met?stase (p=0,033) e o estadiamento cl?nico (p=0,016), localizando-se principalmente no citoplasma das c?lulas estromais. A imunomarca??o da galectina 3, em 87,7% dos casos, correlacionou-se com a presen?a de met?stases (p=0,033) e a grada??o histol?gica de malignidade (p=0,031), sendo encontrada, na maioria dos casos, em carcinoma epiderm?ide de l?ngua de alto grau de malignidade. A galectina 4 n?o exibiu signific?ncia estat?stica com nenhum dos par?metros avaliados. A marca??o da galectina 7, em 73,8% dos casos, exibiu correla??o estatisticamente significativa com a grada??o histol?gica de malignidade (p=0,005), sendo esta marca??o exclusivamente encontrada nas c?lulas neopl?sicas, e na maioria dos casos, encontrada no citoplasma e membrana (50%). A expressiva imunomarca??o das galectinas 1, 3 e 7, verificada em nossa pesquisa, nos leva a sugerir uma ampla participa??o dessas prote?nas na carcinog?nese oral, bem como a sua poss?vel utiliza??o como marcadores do comportamento biol?gico e da progress?o tumoral em carcinoma epiderm?ide de l?ngua

Made available in DSpace on 2014-12-17T15:32:31Z (GMT). No. of bitstreams: 1 GustavoPG_tese.pdf: 864388 bytes, checksum: f88b048184bdf52b12d2e542ee44707e (MD5) Previous issue date: 2005-08-19 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Uma das grandes controv?rsias encontradas na literatura cient?fica consiste no estabelecimento de crit?rios para distin??o entre um fol?culo pericoron?rio espessado e um cisto dent?gero incipiente. O objetivo do presente estudo consistiu em avaliar a express?o imuno-histoqu?mica das integrinas &#945;2&#946;1, &#945;3&#946;1e &#945;5&#946;1 nas referidas entidades, onde foram selecionados 23 casos de fol?culos pericoron?rios espessados e 21casos de cistos dent?geros incipientes. Analisou-se a presen?a ou aus?ncia de express?o destas integrinas nas ilhotas de epit?lio odontog?nico e nos epit?lios constituintes de cada entidade, enfatizando a localiza??o, intensidade e padr?o de distribui??o para compara??o entre as mesmas. Todas as integrinas apresentaram marca??o nos casos analisados. Foi observada uma diferen?a estatisticamente significativa (p<0,0001) para a integrina &#945;2&#946;1 entre as duas entidades, apresentando os cistos dent?geros incipientes uma marca??o mais intensa. Tamb?m se verificou diferen?a entre a camada basal e a suprabasal no epit?lio c?stico (p<0,0034). A integrina &#945;3&#946;1 apresentou uma diferen?a estatisticamente significativa (p<0,013) entre as duas entidades, com os cistos dent?geros incipientes apresentando uma tend?ncia de marca??o intensa. Em rela??o a integrina &#945;5&#946;1, n?o se observou diferen?a de express?o entre os dois grupos, ressaltando-se entretanto a intensa marca??o desta integrina na maioria dos casos aqui avaliados, refor?ando o entendimento da participa??o da mesma na diferencia??o celular. Concluiu-se portanto que a maior express?o da integrina &#945;2&#946;1 em cistos dent?geros incipientes, bem como nas c?lulas da camada basal do epit?lio deste cisto, pode estar relacionada com a maior atividade proliferativa das referidas c?lulas, enquanto que a tend?ncia de express?o mais intensa da integrina &#945;3&#946;1 nos cistos dent?geros incipientes se deva ? participa??o desta integrina na organiza??o da estratifica??o epitelial bem como na expans?o c?stica por poss?vel ativa??o de metaloproteinases. Adicionalmente, verificou-se que estes achados corroboram a possibilidade de distin??o histopatol?gica entre um fol?culo pericoron?rio espessado e um cisto dent?gero incipiente, onde a metaplasia escamosa do epit?lio reduzido do ?rg?o do esmalte para um epit?lio pavimentoso estratificado seria o primeiro sinal vis?vel de transforma??o c?stica

Data de aprova??o ausente. / Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-02-13T18:51:06Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) tatiana_fernandes_araujo_almeida.pdf: 2531556 bytes, checksum: 11a2583c0414dc55d6f907a2566a4de5 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-03-06T12:21:52Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) tatiana_fernandes_araujo_almeida.pdf: 2531556 bytes, checksum: 11a2583c0414dc55d6f907a2566a4de5 (MD5) / Made available in DSpace on 2017-03-06T12:21:52Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) tatiana_fernandes_araujo_almeida.pdf: 2531556 bytes, checksum: 11a2583c0414dc55d6f907a2566a4de5 (MD5) Previous issue date: 2016 / O carcinoma espinocelular oral (CECO) ? uma neoplasia maligna que acomete principalmente idosos. O desencadeamento da doen?a ? relacionado ? exposi??o aos fatores de risco e ao decl?nio da fun??o imune associada ao envelhecimento cronol?gico. Alguns estudos t?m demonstrado um aumento na incid?ncia de CECO em adultos jovens, sugerindo envolvimento de outros fatores na etiologia da doen?a, como uma altera??o do sistema imunol?gico. As c?lulas dendr?ticas (CDs) s?o c?lulas apresentadoras de ant?geno profissionais e estimuladoras eficazes para a expans?o clonal de linf?citos. Acredita-se que uma altera??o da fun??o das CDs em pacientes com c?ncer contribui para a falha da resposta antitumoral, levando a uma consequente progress?o da doen?a. O objetivo deste estudo foi avaliar, atrav?s da imunoistoqu?mica, se h? diferen?a na quantifica??o tissular dos subgrupos de CDs, associada ? idade, em esp?cimes de bi?psias de CECO. Para isso, foram selecionados casos de CECO de pacientes em tr?s diferentes faixas et?rias: G1 (< 40 anos de idade, n = 12), G2 (? 40 at? < 60 anos de idade, n = 15) e G3 (? 60 anos de idade, n = 14). Os marcadores utilizados foram S100, CD1a, CD207 (para CDs imaturas, imCDs), CD83 e CD208 (para CDs maduras, mCDs). As imagens foram capturadas dos campos com maior intensidade de marca??o nas l?minas histol?gicas e a quantifica??o celular foi realizada com o aux?lio do software Image J. A frequ?ncia e localiza??o das CDs foram avaliadas e analizadas estatisticamente nas regi?es intratumoral (intertumoral e ou estromal) e extratumoral. No geral, imCDs foram significativamente mais frequentes que mCDs em todos os grupos. ImCDs e mCDs mostraram preferencialmente localiza??o intratumoral e extratumoral respectivamente. Comparando G1 versus G2 / G3 foi observada um significativo menor n?mero de mCDs em G1. Na compara??o de G1 em rela??o a G2 ou G3 houve um n?mero significativamente menor de ambas, imCDs e mCDs. Nossos resultados mostram que existe uma menor quantidade de imCDs e mCDs em CECO afetando pacientes jovens em compara??o com idosos, sugerindo um comprometimento da resposta imune antitumoral em G1e permitindo a progress?o do tumor. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, [2016]. / Oral squamous cell carcinoma (OSCC) is a malignant neoplasm that mainly affects elderly people. The onset of the disease is related with exposure to risk factors and the decline of immune function associated with chronological aging. Previous studies have demonstrated an increased incidence of CECO in young adults, suggesting involvement of other factors in the etiology of the disease, such as a change in the immune system. Dendritic cells (DCs) are professional antigen presenting cells and effective for stimulating clonal expansion of lymphocytes. It is believed that a change in the function of DC in cancer patients contributes to the failure of the anti-tumor response, leading to a consequent progression of the disease. The aim of this study was to evaluate, by immunohistochemistry, if there were differences in tissue quantification of DCs subsets, associated with age in specimens of OSCC biopsies. For this OSCC cases were selected at three different ages: G1 (< 40 years old, n = 12), G2 (? 40 to <60 years old, n = 15) and G3 (? 60 years old, n = 14). The markers used were S100, CD1a, CD207 (for immature DCs, imDCs), CD83 and CD208 (for mature DCs, mDCs). Images were captured from fields with higher intensity staining in histological sections and cell quantification was performed with Image J software help. The frequency and localization of immunostained DCs were analyzed in intratumoral (intranestal and/or extranestal) and extratumoral areas and statistically compared. Overall, imDCs than mDCs were significantly more frequent in all groups. ImDCs and mDCs showed preferential intratumoral and extratumoral localization, respectivel. Comparing G1 than G2/G3 showed a significant lesser number of mDCs. G1 versus G2 or G3 there is a significantly lower number of both imDCs and mDCs. Our results show a lower number of both imDCs and mDCs in OSCC affecting younger than elderly patients, suggesting impairment of an effective antitumor immune response in G1 and enabling tumor progression, showing a gradual establishment of the antitumor immune response mediated by DCs according to the age, but with defects in quality.

Made available in DSpace on 2015-03-03T15:38:42Z (GMT). No. of bitstreams: 1 BrunaRMS_TESE.pdf: 1789056 bytes, checksum: 3971348cc480ce2ec7b8b377a649483e (MD5) Previous issue date: 2011-02-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Periodontal disease is an inflammatory condition of infectious nature characterized by destruction of protecting and supporting dental tissues. It happens as a response produced by the host when attacked by microorganisms. Several factors are involved in this process. Among them, cytokines are key regulatory molecules in this immune response, playing a role either protective and/or destructive in lesion progression. Thus, this study investigated the immunohistochemical expression of IFN- , GATA-3, IL-17, IL-23, IL-6 and TGF- in gingival tissues of humans, in an attempt to gain a better understanding of the participation of Th1, Th2 and Th17 immune responses in the development of periodontal disease processes. To this end, eighty-two samples of gingival tissues were divided into three groups: Group 1 = 15 (samples of healthy gum tissue as controls), Group 2 = 36 (samples with chronic gingivitis) and Group 3 = 31 (samples with chronic periodontitis). All cases were submitted to morphological analysis from sections stained with hematoxylin and eosin and then subjected to staining by immunohistochemistry using the streptavidin-biotin method. Results showed positive labeling for all proteins. Nonetheless, we observed a greater expression of Th1 cytokines and Th17 cells in group 3. We found statistically significant difference between TGF- expression and the clinical condition of the samples (p=0.02). We conclude that Th1 and Th17 responses may act synergistically in the destructive process of periodontal tissue, overlapping the Th2 response that was also present in these tissues / A doen?a periodontal ? uma condi??o inflamat?ria de car?ter infeccioso, caracterizada pela destrui??o dos tecidos de prote??o e sustenta??o dent?rios, face ? resposta produzida pelo hospedeiro frente ?s agress?es sofridas pelos microrganismos. V?rios fatores est?o envolvidos nesse processo, sendo as citocinas as principais mol?culas reguladoras dessa resposta imune, desempenhando um papel protetor e/ou destrutivo na progress?o da les?o. Diante disso, este experimento investigou a express?o imuno-histoqu?mica de IFN- , GATA-3, IL-17, IL-23, IL-6 e TGF- em tecidos gengivais de humanos, na tentativa de se obter um maior entendimento da participa??o das respostas imunes Th1, Th2 e Th17 no desenvolvimento destes processos patol?gicos. Para tanto, oitenta e duas amostras de tecidos gengivais foram subdivididas em tr?s grupos: Grupo 1=15 (amostras de tecido gengival saud?vel-controle), Grupo 2=36 (amostras com gengivite cr?nica) e Grupo 3=31 (amostras com periodontite cr?nica). Todos os casos foram submetidos ? an?lise morfol?gica a partir de cortes corados em hematoxilina e eosina e, posteriormente, submetidas ? t?cnica de colora??o pela imuno-histoqu?mica atrav?s do m?todo da Estreptoavidina-Biotina. Os resultados mostraram positividade de marca??o para todas as prote?nas, sendo observada uma maior tend?ncia de marca??o para as citocinas das respostas Th1 e Th17 no grupo 3. Diferen?a estatisticamente significativa foi verificada entre a express?o de TGF- e a condi??o cl?nica das amostras (p=0,02). Assim, podemos concluir que as respostas Th1 e Th17 podem atuar sinergicamente no processo destrutivo dos tecidos periodontais, sobrepondo-se ? resposta Th2 que tamb?m se encontrou presente nestes tecidos

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-02-01T21:57:42Z No. of bitstreams: 1 RodrigoGadelhaVasconcelos_TESE.pdf: 1777603 bytes, checksum: 142978c0b438c9eba25a0468ff97275f (MD5) / Approved for entry into archive by Elisangela Moura (lilaalves@gmail.com) on 2016-03-07T23:32:33Z (GMT) No. of bitstreams: 1 RodrigoGadelhaVasconcelos_TESE.pdf: 1777603 bytes, checksum: 142978c0b438c9eba25a0468ff97275f (MD5) / Made available in DSpace on 2016-03-07T23:32:33Z (GMT). No. of bitstreams: 1 RodrigoGadelhaVasconcelos_TESE.pdf: 1777603 bytes, checksum: 142978c0b438c9eba25a0468ff97275f (MD5) Previous issue date: 2014-12-18 / A les?o perif?rica de c?lulas gigantes (LPCG) e a les?o central de c?lulas gigantes (LCCG) s?o les?es histologicamente semelhantes que acometem a regi?o de cabe?a e pesco?o. O estudo teve a finalidade de analisar a express?o imuno-histoqu?mica atrav?s dos marcadores GLUT-1, GLUT-3 e M-CSF em uma s?rie de casos de les?o perif?rica e central de c?lulas gigantes, na tentativa de estabelecer poss?veis associa??es e correla??es entre a express?o destas prote?nas nessas les?es, buscando uma melhor compreens?o do diferente comportamento biol?gico dessas entidades patol?gicas. A amostra foi constitu?da por 20 esp?cimes teciduais emblocados em parafina de LPCG, 20 de LCCG n?o agressivo e 20 de LCCG agressivo, oriundos do Servi?o de Anatomia Patol?gica da Disciplina de Patologia Oral do Departamento de Odontologia da UFRN. Em rela??o ao GLUT-1, verificou-se uma diferen?a estatisticamente significante (p< 0,05) na quantidade de c?lulas mononucleares imunomarcadas entre a les?o perif?rica (LP) e a les?o central n?o agressiva (LCNA) e entre a LP e a les?o central agressiva (LCA). Em rela??o ? intensidade da marca??o tamb?m foi verificado uma diferen?a estatisticamente significante tanto para as c?lulas mononucleares quanto para as c?lulas gigantes entre LP e LCNA e entre LP e LCA, nas c?lulas gigantes tamb?m ocorreu uma diferen?a estatisticamente significante entre a LCNA e a LCA. Em rela??o ao GLUT-3, foi encontrada uma diferen?a estatisticamente significante entre LP e LCA e entre LCNA e LCA na quantidade de c?lulas mononucleares imunomarcadas. No que concerne ? intensidade de marca??o para a referida prote?na foi verificado uma diferen?a estatisticamente significante nas c?lulas gigantes entre LP e LCA. Para o M-CSF foi observado apenas uma diferen?a estatisticamente significante na intensidade de marca??o nas c?lulas mononucleares entre LP e LCNA e entre LP e LCA. Com base nestes resultados, pode-se concluir a participa??o do GLUT-1, GLUT-3 e do M-CSF na patog?nese das les?es estudadas. Os transportadores de glicose estariam envolvidos no fornecimento de energia, para o metabolismo energ?tico das c?lulas e a prote?na osteoclastog?nica estaria envolvida no mecanismo de reabsor??o ?ssea encontrada nessas les?es. / The peripheral giant cell lesion ( PG CL ) and the central giant cell lesion ( CGC L) are lesions histologically similar affecting the head and neck region . The study aimed to analyze the immunohistochemical expression of markers GLUT - 1 , GLUT - 3 and M - CSF in a series of cases of PGCL and CGCL , in trying to understand the different biological behavior of these pathologies . The sample consisted of 20 tissue specimens of PGCL 20 central lesion of not aggressive giant cell ( CLNAGC) and 20 central lesi on of aggressive giant cell ( CLAGC), coming from the Pathology Unit of Oral Pathology of the Department of Dentistry of UFRN . W as performed the s emi - quantitative and qualitative analysis of immunohistochemical expression of the markers in giant cells and m ononuclear cells . In relation to the GLUT - 1, it was found a statistically significant difference (p < 0.05) in the number of mononuclear cells immunomarked between the PGCL and the CLNAGC and between the PGCL and CLAGC . Regarding the intensity of staining w as also observed a statistically significant difference both at the mononuclear cells as in giant cells between PL and CLNAGC and between PGCL and CLAGC , at the giant cells there was also a statistically significant difference between the CLNAGC and CLAGC . In relation to GLUT - 3 , was found a statistically significant difference between PGCL and CLAGC and between CLAGC and CLNAGC in amount of mononuclear cells immunomarked . Regarding the intensity of labeling for such protein was found a statistically signifi cant difference at the giant cells between PL and CLAGC . To the M - CSF was observed only a statistically significant difference in the intensity of labeling at the mononuclear cells between PGCL and CLNAGC and between PGCL and CLAGC . Based on these results, we can conclude the participation of GLUT - 1, GLUT - 3 and M - CSF in the pathogenesis of the lesions studied. The bigger immunostaining of these proteins in mononuclear cells show that these cells perform a higher metabolic activity and osteoclastogenic, espe cially in CLAGC . It was found that the mononuclear cells were more related to the pathogenesis of the studied lesions than properly the giant s cell s.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-02-29T22:55:50Z No. of bitstreams: 1 EmiliaBeatrizDasNevesSilvaMaiaPimentel_TESE.pdf: 2954281 bytes, checksum: 39a346f6b0d47bccce334327ee69b78b (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-02T19:34:04Z (GMT) No. of bitstreams: 1 EmiliaBeatrizDasNevesSilvaMaiaPimentel_TESE.pdf: 2954281 bytes, checksum: 39a346f6b0d47bccce334327ee69b78b (MD5) / Made available in DSpace on 2016-03-02T19:34:04Z (GMT). No. of bitstreams: 1 EmiliaBeatrizDasNevesSilvaMaiaPimentel_TESE.pdf: 2954281 bytes, checksum: 39a346f6b0d47bccce334327ee69b78b (MD5) Previous issue date: 2015-02-27 / Tumores odontog?nicos prov?m de tecidos dent?rios por prolifera??o de tecido epitelial e/ou mesenquimal. Biologicamente, estas les?es poder ter naturezas distintas, sendo caracterizadas como altera??es no desenvolvimento tecidual (hamartomas), tumores benignos n?o agressivos ou agressivo e tumores malignos. No desenvolvimento e na progress?o desses tumores odontog?nicos, diferentes rela??es de intera??es epit?lio/mesenquimais ocorrem originando os diferentes tipos dessas les?es. Numerosas mol?culas sinalizadoras participam dessas rela??es, dentre estas o fator de crescimento fibrobl?stico (FGF), e a prote?na (?ssea Morfog?nica (BMP) e proteoglicanos de sulfato de heparan (sindecan), as mol?culas envolvidas nos processos de transcri??o e os produtos transcritos. Diante, objetivo dessa pesquisa foi investigar a imunolocaliza??o de fatores de crescimento (BMP-4 e FGF-8) e de prote?na mesenquimal (Sindecan-1) em uma s?rie de tumores odontog?nicos apresentando comportamento biol?gicos distintos, visando contribuir para um melhor entendimento da participa??o dessas proteinas no desenvolvimento tumoral. A amostra foi constitu?da por 21 ameloblastomas do tipo s?lido, 19 cerataocistos odontog?nicos e 14 tumores odontog?nicos adenomat?ides. As c?lulas imunomarcadas por BMP-4 e FGF-8 foram quantificadas, enquanto a contagem de sindecan-1 foi semi-quantitativa, e cada caso tumoral catergorizado em escores: 0 - ausente; 1 - 1 a 10% de c?lulas positivas, 2 - 11 a 50% de c?lulas positivas; e 3 - > < 50% de c?lulas positivas. Maior imunoexpress?o da sindecan-e foi observada no epit?lio das les?es quando comparada com o mesenquima. No ameloblastoma e o ceratocisto odontog?nico essa express?o foi maior que no TOA, o que pode caracterizar um comportamento biol?gico mais agressivo dessas duas primeiras les?es. A maior express?o de BMP-4 no mesenquima de ameloblastoma comparado ao ceratocisto ( p=0,009), pode indicar uma intera??o e participa??o ativa nas c?lulas parenquimais na patogenese desses tumores, enquanto que no tecido epitelial, nenhuma diferen?a signigicativa foi observada quando comparadas as tr?s les?es. Sendo que no ameloblastoma sua express?o foi predominantemente mesenquimal (p=0,008), enquanto no ceratocisto maior express?o foi observada no epit?lio (p = 0,0046). Em todas as les?es, correla??o forte ou moderada foi observada na imunoexpress?o de BMP-4 no epit?lio e mesenquima. Para FGF-8, em nenhuma les?o foi observaa diferen?a entre a imunoexprss?o no epit?lio ou mesenquima, contudo no ameloblastoma correla??o positiva foi encontrada (Correla??o Spearman, rho=0,857,p<0,001), indicando que a imunoexpress?o de FGF-8 concomitante foi indicar um pior progn?stico para ameloblastomas e tamb?m, estar a uma associado a uma maior atividade osteol?tica obsrervada nesses tumores. Concluiu-se ent?o que os tr?s biomarcadores avaliados nesse estudo (BMP-4, FGF-8 e Sindecan) participam ativamente da patogenese das les?es, sendo que maior imunoexpress?o de FGF-8 e sindecan pode estar associada a um comportamento biol?gico mais agressivo, enquanto BMP-4 apresentou padr?o de imunoexpress?o semelhante nas tr?s les?es, podendo estar associado ? diferencia??o celular e manuten??o do padr?o de crescimento da les?es. / The development and progression of odontogenic tumors have been associated with an imbalance in the activity of growth factors, adhesion molecules, extracellular matrix proteins and their degradation enzymes, angiogenic factors and osteolytic. Some studies have shown that interaction relationships inductive epithelial / mesenchymal determinants of Odontogenesis are mimicked by these tumors. The objective of this research was to investigate the immunolocalization of growth factors (BMP-4 and FGF-8) and Sindecan-1 structural protein in a series of odontogenic tumors presenting different biological behaviors, to contribute to a better understanding of the role of these proteins in tumor development. The sample consisted of 21 of the solid ameloblastoma, odontogenic keratocysts 19 and 14 odontogenic adenomatoid tumors. Increased Sindecan-1 immunostaining was seen in the epithelium of the lesions when compared with mesenchyme. In ameloblastoma and odontogenic keratocysts, this expression was higher than in AOT. Epithelial expression of BMP4 showed quantitatively similar in the three studied lesions; however, when anlisada mesenchymal immunoreactivity, was detected significant higher expression when compared to the ameloblastoma keratocysts. In ameloblastoma, mesenchymal expression was predominantly (p = 0.008), while in keratocyst higher expression in the epithelium was observed (p = 0.046). In all injuries, strong or moderate correlation was observed in the BMP-4 immunoreactivity in the epithelium and mesenchyme. FGF-8, no injury was observed difference between the immunoreactivity in the epithelium or mesenchyme, however in ameloblastoma positive correlation was found (Spearman correlation, rho = 0.857, p <0.001). The results of this study suggest that the three evaluated biomarkers actively involved in the pathogenesis of lesions, especially the expression of ameloblastomas indicating a strong interaction between parenchymal and stromal cells which may contribute to its marked aggressiveness.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-07-11T17:13:30Z No. of bitstreams: 1 MariannaSampaioSerpa_DISSERT.pdf: 1809045 bytes, checksum: 8e36684515735c29c16965b8e9759877 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-07-15T19:53:50Z (GMT) No. of bitstreams: 1 MariannaSampaioSerpa_DISSERT.pdf: 1809045 bytes, checksum: 8e36684515735c29c16965b8e9759877 (MD5) / Made available in DSpace on 2016-07-15T19:53:50Z (GMT). No. of bitstreams: 1 MariannaSampaioSerpa_DISSERT.pdf: 1809045 bytes, checksum: 8e36684515735c29c16965b8e9759877 (MD5) Previous issue date: 2016-02-12 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O carcinoma epidermoide oral (CEO) ? a neoplasia maligna mais comum da cavidade oral, apresentando uma alta taxa de mortalidade. Devido a isto, a descoberta de biomarcadores que facilitem a compreens?o do comportamento biol?gico desse tumor e aprimorem o tratamento ? necess?rio. O ativador de plasminog?nio do tipo uroquinase (uPA) e o seu receptor, uPAR, t?m se destacado por atuarem na prote?lise de estruturas da membrana basal e matriz extracelular, facilitando a invas?o tumoral. O presente estudo se prop?s a avaliar a imunoexpress?o dessas prote?nas em 46 casos de carcinoma epidermoide de l?ngua oral (CELO). Esses resultados foram relacionados com a presen?a de met?stase, estadiamento cl?nico TNM, recidiva locoregional, desfecho da les?o e grada??o histol?gica de malignidade. A imunomarca??o de cada caso foi avaliada semiquantitativamente, tanto no front de invas?o como no centro do tumor, na qual foram atribu?dos os escores: 0 (0% de c?lulas positivas), 1(1-10% de c?lulas positivas), 2 (11-50% de c?lulas positivas), 3 (mais de 50% de c?lulas positivas). A express?o do uPA foi observada em 93,5% dos casos no front de invas?o, com predom?nio do escore 2 (34,8%), e em 67,9% dos casos no centro do tumor, com predom?nio do escore 1 (32,6%). De modo geral, os par?metros cl?nicos n?o exerceram influ?ncia na imunoexpress?o do uPA. Em rela??o ? grada??o histol?gica, foi observada uma maior express?o de uPA nos casos de alto grau de malignidade em rela??o aos de baixo grau de malignidade (p=0,05). Quando analisado em rela??o aos par?metros morfol?gicos, foi identificado uma maior express?o do uPA nos casos de pior padr?o de invas?o (p=0,03). A express?o do uPAR foi observada em 73,9% dos casos no front de invas?o, com predom?nio do escore 1 (45,65%), e em 47,5% dos casos no centro do tumor, com predom?nio do escore 0 (54,35%). Embora n?o tenham sido observadas signific?ncias estat?sticas em rela??o ? met?stase linfonodal, estadiamento cl?nico TNM, desfecho e grada??o histol?gica, houve uma maior express?o do uPAR nos casos com recidiva locoregional em rela??o aos sem recidiva (p=0,04). Em rela??o ? an?lise da localiza??o tumoral, foi observada uma maior express?o de uPA e uPAR no front de invas?o em rela??o ao centro do tumor (p<0,001). Na correla??o entre uPA e uPAR, n?o foi observada signific?ncia estat?stica. Com base nestes resultados, sugere-se que o uPA e uPAR estejam envolvidos na progress?o do CELO, atuando principalmente na regi?o mais profunda do tumor. / Squamous cell carcinoma (SCC ) is the most common malignancy of the oral cavity (OSCC), with a high mortality rate. Due to this, the discovery of biomarkers that facilitate the understanding of the biological behavior of the tumor and improve treatment is necessary. Urokinase type plasminogen activator (uPA) and its receptor, uPAR, are responsible for the proteolysis of structures of the basement membrana and extracellular matrix, facilitating tumor invasion. This study aims to assess the immuno expression of these proteins in 46 cases of squamous cell carcinoma of the oral tongue (OTSCC). These results were related to the presence of metastasis, clinical TNM staging, locoregional recurrence, outcome of the lesion and histological grading. Immunostaining of each case was evaluated semiquantitatively, in the front of invasion and center of the tumor, in which scores were assigned: 0 (0% of positive cells), 1 (1-10% of positive cells), 2 (11 -50% positive cells) and 3 (more than 50% positive cells). The expression of uPA was observed in 93.5% (n=43) of the cases in the front of invasion, with predominance of score 2 (n=16; 34.8%) and in 67.9% (n=31) of the cases in the center of the tumor, with predominance of score 1 (n=15; 32.6%). Overall, the immunoexpression of uPA was not associated with clinical parameters. Regarding the malignant histological grading, a higher expression of uPA was observed in cases of high-grade malignancy comp ared to low-grade malignancy (p=0.05). Regarding the morphological parameters, increased expression of uPA was observed in the worst mode of invasion (p=0.03 ). The expression of uPAR was observed in 73.9% of cases in the front of invasion, with a predominance of score 1 (n=21; 45.6 %), and in 47.5% (n=21) of the cases in the center of the tumor, with a predominance of score 0 (n=25; 54.4%). Although no statistical differences were observed in relation to lymph node metastasis, clinical TNM staging, outcome, and histological grading, there was a higher expression of uPAR in cases with locoregional recurrence (p=0.04). Regarding the tumor intra -localization, it was observed an increased expression of uPA and uPAR at the front of invasion in relation to the center of the tumor (p<0.001). Regarding the correlation between uPA and uPAR, there was no statistical sign ificance. Based on these results, it is suggested that uPA and uPAR are involved in the progression of CELO, mainly in the deeper region of the tumor.

Made available in DSpace on 2014-12-17T15:32:16Z (GMT). No. of bitstreams: 1 MarceloGV.pdf: 2007167 bytes, checksum: 5ee7e9c9bd56fd72fecb1874fb64a80e (MD5) Previous issue date: 2008-02-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Angiogenesis, a fundamental mechanism in tumor development, is used for differential diagnosis and prognosis purposes in various neoplasias of the head and neck. This study proposes to assess angiogenic activity using immunohistochemical expression by anti-CD105 and anti-CD34 antibodies in 20 cases of hemangiomas and 20 cases of oral pyogenic granulomas, in addition to determining the usefulness of these markers as one of the differential diagnosis resources for these two oral lesions. The results showed no statistically significant difference between microvascular count (MVC) means determined by anti-CD105 (p = 0.803) and anti-CD34 (p = 0.279) antibodies. The mean number of vessels obtained by MVC in the oral hemangiomas immunostained by anti-CD105 and anti-CD34 was 18.75 and 59.72, respectively, whereas in the oral pyogenic granulomas, the mean number was 20.22 and 48.09 respectively. It was also shown that CD34 was more effective than CD105 in identifying blood vessels. However, it must be pointed out that the anti-CD105 antibody seems to be more related to vascular neoformation. Overall, this assay reinforces the role of angiogenic factors in the etiopathogenesis of hemangiomas and oral pyogenic granulomas, but the results showed that angiogenesis quantification cannot be used as a differential diagnosis parameter between the two lesions analyzed / A angiog?nese ? um mecanismo fundamental para o desenvolvimento tumoral, sendo utilizada para fins de diagn?stico diferencial e determina??o de progn?stico em v?rias neoplasias de cabe?a e pesco?o. Este trabalho se prop?s avaliar a atividade angiog?nica atrav?s da express?o imuno-histoqu?mica dos anticorpos anti-CD105 e anti-CD34 em 20 casos de hemangiomas e 20 casos de granulomas piog?nicos orais, al?m de averiguar a utilidade destes marcadores como um dos recursos de diagn?stico diferencial para estas duas les?es orais. Os resultados deste experimento demonstraram que n?o houve diferen?a estatisticamente significativa entre as m?dias de contagem microvascular (MVC) determinadas pelos os anticorpos anti-CD105 (p=0,803) e anti-CD34 (p=0,279). O n?mero m?dio dos vasos obtidos pela MVC nos esp?cimes de hemangiomas orais imunomarcados pelo anti-CD105 e anti-CD34 foram respectivamente 18,75 e 59,72, enquanto nos granulomas piog?nicos orais, o n?mero m?dio dos vasos obtidos pela MVC pelo anti-CD105 e anti-CD34 foram respectivamente 20,22 e 48,09. Foi verificado, tamb?m, que o CD34 foi mais efetivo na identifica??o de vasos sang??neos quando comparado com o CD105. Entretanto, faz-se necess?rio destacar, que o anticorpo anti-CD105 parece estar mais relacionado com a neoforma??o vascular. Em linhas gerais, este ensaio refor?a a participa??o dos fatores angiog?nicos na etiopatog?nese dos hemangiomas e granulomas piog?nicos orais, por?m os resultados mostraram que a quantifica??o da angiog?nese n?o pode ser utilizada como par?metro de diagn?stico diferencial entre as duas les?es analisadas