O fenômeno metafórico, caro às ciências da linguagem, tem recentemente adentrado também os estudos cognitivos, visto que se tem advogado que a metáfora não ocorre apenas na linguagem, mas constitui processos do pensamento. Argumento aqui que a metaforização articula singularidades que operam na contiguidade entre linguagem e cognição e, nela, na invenção de si e do mundo. Para tanto, um projeto de pesquisa-intervenção foi cartografado, através da criação de um método com retalhos, denominado pat(c)hwork. No projeto, desenvolvido pelo Núcleo de Pesquisas em Ecologias e Políticas Cognitivas (NUCOGS-UFRGS), um jogo digital móvel baseado em localização foi produzido para ser jogado no Parque Jardim Botânico de Porto Alegre, em um contexto não formal de aprendizagem. O jogo foi testado por estudantes do 4º e 5º ano do ensino fundamental de uma escola pública conveniada com o parque e com o grupo de pesquisa, através de oficinas. O foco cartográfico enfatizou o linguajar como agir humano na linguagem, com especial atenção à hipótese de que o mesmo caracteriza-se também pela metaforicidade O referencial teórico apóia-se na teoria autopoiética, por meio da concepção de linguagem como linguajar, entrelaçada ao emocionar e ao conversar, compondo redes de conversação; na teoria da enação, mediante a noção de cognição incorporada; na linguística cognitiva, especialmente na teoria da metáfora conceitual, como processo de fabricação e organização que pressupõe a corporeidade; e na cognição inventiva, pela costura dos conceitos de políticas, ecologias e tecnologias cognitivas. Ao cartografarmos retalhos linguageiros de conexões entre os domínios físico e digital, necessários à jogabilidade de um jogo móvel de localização, esperamos contribuir para uma compreensão do fenômeno metafórico como modulador da linguagem e da cognição como ações corporificadas, emergentes e acentradas, processos e produtos de recorrências e recursões operadas no entrelaçamento linguajar-conhecer ao longo do viver. / The metaphorical phenomenon, dear to the sciences of language, has also recently entered the cognitive studies, since it has been argued that metaphor does not occur only in language, but constitutes processes of thought. I argue here that metaphorization articulates singularities that operate in the contiguity between language and cognition and, within it, in the invention of oneself and of the world. For this, a research-intervention project was mapped through the creation of a patchwork method, called pat(c)hwork. In the project, developed by the research group Núcleo de Ecologias e Políticas Cognitivas (NUCOGS-UFRGS), a location-based mobile game was produced to be played at the Botanical Garden of Porto Alegre, in a non-formal learning context. The game was tested by students from the 4th and 5th grades of an elementary public school that entered a partnership with the park and the research group, through workshops. The cartographic focus emphasized languaging as human action in language, with special attention to the hypothesis that it is also characterized by metaphoricity The theoretical research framework is based on autopoietic theory, through the conception of language as languaging, intertwined with emotion and conversation, composing conversation networks; on enactivism, through the notion of incorporated cognition; on cognitive linguistics, especially on the theory of conceptual metaphor, as a process of fabrication and organization that presupposes corporeality; and on inventive cognition, by sewing the concepts of cognitive policies, ecologies and technologies. By mapping patches of connections between the physical and the digital domains, necessary for the gameplay of a location-based mobile game, we hope to contribute to an understanding of the metaphorical phenomenon as a modulator of embodied, emerging and decentralized language and cognition as modes of actions, processes and products of recurrences and recursions operated in the intertwining of languaging and knowing along living.

This diploma paper focuses on progress of the pharish in Horažďovice in years 1251 {--} 1850. It was the incorporate pharish of the Sovereign Order of Malta, to wich was not given the research interest before now. This thesis tries to discover the chronologic progress and changes of the parish in the watched period, based primarily on the resources of archives. Further tries to see into the situation of incorporated pharish in terms of dioceses and archidioceses. The work is divided into nine chapters. After the introduction with definition the theme theme of the research and evaluation the used sources succeeds a short chapter about the Order, its history and hierarchy abroad also in the czech province. Following four chapters (forming the main part of the thesis) inscribe the six-hundred long progress of the pharish in Horažďovice. The next part is devoted to the parsons, the parish church St. Peter and Paul and its asset. The ninth chapter dissertates about the situation of the monastic incorporated pharish in the arcidiocese of Prague and the diocese of České Budějovice. This situation is illustrated on example of the Maltese pharish in Horažďovice. The keynotes of the archival research summarizes the conclusion, wich is followed by a list of the abbreviations used, sources and the literature and the annexes.

The United States and South Africa, exemplars of "archsegregation," have been constituted within an arc of historical racialized delineations which began with the centering, and subsequent overrepresentation, of European maleness and whiteness as the sole definition of Man. Globally present and persistent, these racialized delineations have been localized and spatially embedded through the tools of urban planning. This arc of racialized otherness, ineffectively erased, continues to inform the racially differentiated geospatial, health, social, and economic outcomes in contemporary urban form and functions for Black communities. It is within this historical arc, and against these differentiated outcomes, that contemporary urban discourse and contestation between individuals and institutions are situated. This historical othering provides not just a racialized geo-historical contextualization, but also works to preclude the recognition of the some of the most vulnerable urban community members. As urbanists and advocates strive to co-create urban space and place with municipalities, meeting the needs of these residents is imperative. In order to meet these needs, their lived experiences, and voices must be fully recognized and engaged in the processes and programs of urban co-creation, including in digital spaces and forums. Critical to achieving recognition acknowledging and situating contemporary digital discourses between local municipalities, Black residents, and Black networks within this historically racialized arc is necessary. In doing so, explore if, and how, race, specifically Blackness, is enacted in municipal digital discourse, whether these enactments serve to advance or impede resident recognition and participation, and how Black users, as residents and social network curators, engage and respond to these municipal discursive enactments. This exploratory research is a geographically and digitally multi-sited incorporated comparison of Chicago, Illinois, and Johannesburg South Africa. Using Twitter and ethnographic data collected between December 1, 2019, and March 31, 2020, this research layers digital ethnographic mixed methods and qualitive mixed methods, including traditional ethnographic, digital ethnographic, grounded theory, social change and discourse analysis, and frame analysis to explore three research goals. First, explore the digital discursive practices and frames employed by municipalities to inform, communicate with, and engage Black communities, and, if and how, these frames are situated within a historically racialized arc. Second, identify the ways in which Black residents, in dual discursive engagements with local municipalities and their own social networks, interact and engage with the municipal frames centering on Blackness. Third, through ethnographic narratives, acknowledge the marginalized residents of the Central Business District of Johannesburg, South Africa as "agents of knowledge," with critical and valuable knowledge claims which arise from their lived experiences anchored within racialized place and space. In doing so, support the efforts of these residents in recentering the validity of their knowledge claims in the co-creation of urban place and space. Additionally, in situating the city within a historically racialized arc develop novel frameworks, the racialized palimpsest city and syndemic segregation, through which to explore contemporary urban interactions and engagements. / Doctor of Philosophy / The United States and South Africa, exemplars of "archsegregation," have been constituted within an arc of historical racialized delineations which began with the centering, and subsequent overrepresentation, of European maleness and whiteness as the sole definition of Man. Globally present and persistent, these racialized delineations have been localized and spatially embedded through the tools of urban planning. This arc of racialized otherness, ineffectively erased, continues to inform the racially differentiated geospatial, health, social, and economic outcomes in contemporary urban form and functions for Black communities. It is within this historical arc, and against these differentiated outcomes, that contemporary urban discourse and contestation between individuals and institutions are situated. This historical othering provides not just a racialized geo-historical contextualization, but also works to preclude the recognition of the some of the most vulnerable urban community members. As urbanists and advocates strive to co-create urban space and place with municipalities, meeting the needs of these residents is imperative. In order to meet these needs, their lived experiences, and voices must be fully recognized and engaged in the processes and programs of urban co-creation, including in digital spaces and forums. Critical to achieving recognition acknowledging and situating contemporary digital discourses between local municipalities, Black residents, and Black networks within this historically racialized arc is necessary. In doing so, explore if, and how, race, specifically Blackness, is enacted in municipal digital discourse, whether these enactments serve to advance or impede resident recognition and participation, and how Black users, as residents and social network curators, engage and respond to these municipal discursive enactments. This exploratory research is a geographically and digitally multi-sited incorporated comparison of Chicago, Illinois, and Johannesburg South Africa. Using Twitter and ethnographic data collected between December 1, 2019, and March 31, 2020, this research layers digital ethnographic mixed methods and qualitive mixed methods, including traditional ethnographic, digital ethnographic, grounded theory, social change and discourse analysis, and frame analysis to explore three research goals. First, explore the digital discursive practices and frames employed by municipalities to inform, communicate with, and engage Black communities, and, if and how, these frames are situated within a historically racialized arc. Second, identify the ways in which Black residents, in dual discursive engagements with local municipalities and their own social networks, interact and engage with the municipal frames centering on Blackness. Third, through ethnographic narratives, acknowledge the marginalized residents of the Central Business District of Johannesburg, South Africa as "agents of knowledge," with critical and valuable knowledge claims which arise from their lived experiences anchored within racialized place and space. In doing so, support the efforts of these residents in recentering the validity of their knowledge claims in the co-creation of urban place and space. Additionally, in situating the city within a historically racialized arc develop novel frameworks, the racialized palimpsest city and syndemic segregation, through which to explore contemporary urban interactions and engagements.

Cette thèse interroge le rôle de l'école dans la construction identitaire des adolescents. La langue est au cœur de cette construction identitaire. Cependant, dans le paysage scolaire français, les frontières entre la langue de scolarisation, les langues des familles, le FLE et les LVE sont quasi hermétiques et contribuent au morcellement identitaire. Les didactiques compartimentées induisent de nombreuses dissociations (langue et culture, corps et esprit) qui sont un frein à l'élaboration de liens entre l'élève et son environnement. Les élèves rendus vulnérables finissent par accumuler des difficultés et l'école devient un lieu d'exclusion sociale.Face à la nécessité de penser une pédagogie permettant aux élèves de vivre l'altérité linguistique et culturelle en termes de reliance, cette thèse propose l'analyse de l'impact de scénarios artistiques en classe d'anglais. Ces scénarios qui ont été conçus avec des élèves de troisième dans un collège de Bobigny s'appuient sur leurs expériences interculturelles et transculturelles, plurilingues ainsi que leurs interprétations du monde. Ils sont fondés sur la co-réflexion, la co-écriture, la co-création et le co-sentir à travers la danse et le théâtre et ouvrent sur un espace qui offre la possibilité de verbaliser ses émotions.L'analyse des scénarios pédagogico-didactiques qui font l'objet de ce travail s'appuie sur un triple ancrage : ethnopsychanalytique (clinique transculturelle, MR. Moro) psychologique (création d'une niche sécurisante à l'école (B. Cyrulnik) au travers d'un apprentissage coopératif, (D. Johnson et R. Johnson) et didactique, au travers d'une médiation artistique (apprentissage incorporé au travers des arts, J. Aden). Ces trois champs disciplinaires sont complémentaires et les scénarios visent à rétablir un lien entre les processus cognitifs et émotionnels.Une pédagogie transculturelle permet de repenser la classe en communauté « expérientielle » (communauté interprétative, coopérative et une communauté de pratique) tout en respectant et valorisant la singularité de chacun. Au cœur de cette pédagogie, la notion d'expérience et de parole partagée est centrale. Le dispositif analysé est à l'interface d'expériences esthétiques collectives et singulières et permet d'opérer un glissement de perspective sur : la représentation de soi, la représentation de l'autre, la relation entre soi et l'autre, la relation entre soi et son environnement, la relation entre l'autre et son environnement et, in fine, la prise de conscience de soi au sein de la matrice sociale.Cette thèse à visée exploratoire repose sur une méthodologie mixte à l'interface de la clinique transculturelle, de la psychologie et de la didactique des langues-cultures ; elle analyse les effets d'une pédagogie transculturelle au travers de la parole des élèves recueillie pendant le projet et à plus long terme, trois années après l'expérimentation. Le corpus est composé d'interactions de classe en anglais et en français, de pièces en anglais écrites et jouées par les élèves à la fin de l'année d'expérimentation ainsi que des entretiens qualitatifs réalisés a postériori visant à mesurer les impacts à moyen terme de cette expérience d'acteurs au sein d'une communauté expérientielle à un moment précis de l'adolescence. / This thesis questions the contribution of the school system to adolescent identity formation. Language is at the heart of this construction. However, in the French school landscape, the boundaries between the language of schooling, language families, the ELF and LVE are near hermetic and contribute to the fragmentation of identity. Compartmentalized teaching induces many dissociations (language and culture, body and mind) that hinder the development of ties between students and their environment. Vulnerable pupils accumulate difficulties and school becomes a place of social exclusion.Faced with the necessity of thinking pedagogy for pupils to experiencelinguistic and cultural otherness in terms of interrelatedness, this thesis proposes an analysis of the impact of artistic scenarios in English class. These scenarios were designed for students in a secondary school in Bobigny and they are based on their intercultural, transcultural and multilingual experiences and their interpretations of the world. They are built on co-reflection, co-writing, co- creation and co-feeling through dance and drama and open onto a space that offers the opportunity to verbalize their emotions. The analysis of pedagogical-artistic scenarios that are the subject of this work is based on a triple settings: ethnopsychological (transcultural psychotherapy, MR. Moro) psychological (creating a reassuring atmosphere which makes adolescents feel secure, B. Cyrulnik and cooperative learning, D. Johnson and R. Johnson) and pedagogical (incorporated learning, J. Aden). This triple setting offers a transcultural mediation as a method, to use the potential of artistic expression and creativity of adolescents. These three disciplines are complementary and the scenarios are designed among other things to restore a link between cognitive and emotional processes.A transcultural pedagogy allows us to rethink the class into an experiential community (interpretative and cooperative community, and a community of practice) while respecting and valuing the uniqueness of each pupil. At the heart of this pedagogy, the notion of shared experience and speech is central. The device is analyzed at the interface of collective and singular aesthetic experience and allows a shift in perspective on: self-representation, the representation of the other, the relationship between self and other, the relationship between oneself and his or her environment, the relationship between the others and their environment and in fine the awareness of self within the social matrix.This thesis is based on a mixed methodology and is at the interface of transcultural clinic, psychology and teaching of languages and cultures. It analyzes the impact of cross-cultural education through the voices of students collected during the project and in the longer term, three years after the experiment. The corpus is composed of class interactions in English and French, plays in English written and performed by students at the end of the year of the experimentation and qualitative interviews conducted afterwards.

This diploma thesis discusses the implementation of a GCC target for the Texas Instruments TMS320C6000 DSP platform. To this end, it makes use of mechanisms offered by GCC for porting to new target architectures. GCC internals such as the handling of conditional jumps and the layout of stack frames are investigated and applied to the new architecture. / Diese Diplomarbeit behandelt die Implementierung eines GCC-Targets für die DSP-Plattform TMS320C6000 von Texas Instruments. Dazu werden Mechanismen genutzt, die GCC für die Portierung auf neue Zielplattformen anbietet. GCC-Interna, wie die Behandlung bedingter Sprünge und das Layout von Stack-Frames, werden untersucht und auf die neue Architektur angewendet.

This diploma thesis discusses the applicability of GCC optimization algorithms for the TI TMS320C6x processor family. Conditional and Parallel Execution is used to speed up the resulting code. It describes the optimization framework of the GCC version 4.0 and the implementation details. / Diese Diplomarbeit behandelt die Anwendbarkeit der verschiedenen GCC Optimierungsalgorithmen für die TI TMS320C6x Prozessorfamilie. Bedingte und parallele Ausführbarkeit werden zur Beschleunigung eingesetzt. Sie beschreibt den Rahmen in dem die Optimierungen in Version 4.0 des GCC stattfinden und Details zur Implementierung.

Pour compléter leur cycle de vie, les virus interagissent avec de nombreux facteurs de la cellule-hôte. Le virus Herpès simplex de type 1 (HSV-1) ne fait pas exception. Une récente étude protéomique du virus effectuée par notre laboratoire a permis d’identifier 49protéines cellulaires potentiellement incorporées dans les virions matures d’HSV-1 [1]. Étant donné que certaines de ces protéines peuvent jouer des rôles importants au cours du cycle de vie du virus, elles constituent des cibles de choix pour identifier et caractériser de nouvelles interactions hôte-pathogène dans le contexte d’HSV-1. D’ailleurs le laboratoire a été effectué un criblage aux petits ARN d’interférence qui a démontré qu'au moins 15 des protéines incorporées sont impliqués dans le cycle de réplication de HSV-1 en culture cellulaire (Annexe 1). Des nombreuses études rapportent l'incorporation des protéines de l'hôte dans les virions matures mais très peu abordent l'importance de la fraction des protéines cellulaires incorporée dans les virions pour le cycle virale. Pour vérifier ça, nous avons déplété ces protéines des virions matures extracellulaires en utilisant des petits ARN d’interférence. Par la suite, nous avons utilisé ces virus déplétés pour réinfecter des cellules déplétées ou normales. Cette méthode nous a permis d'identifier pour la première fois 8 protéines (DDX3X, HSPA8, KRT10, MIF, Rab5A, Rab6A, Rab10 et 14-3-3ζ) dont l'absence dans les virions réduit la production virale d'au moins 50%. Pour mieux comprendre à quelle étape du cycle viral ces protéines sont nécessaires, nous avons aussi quantifié les virus intracellulaires, produits des cellules déplétées individuellement des quinze protéines cellulaires. Ainsi, nous avons trouvé que dans nos conditions 7 de ces 8 protéines cellulaires (DDX3X, HSPA8, KRT10, MIF, Rab5A, Rab6A et Rab10) semblent impliquées dans la production des virus intracellulaires, ce qui nous a stimulés à débuter une série de tests plus approfondis de l’entrée d’HSV-1. Les résultats préliminaires, démontrent l’implication dans l’entrée d’HSV-1 d’au moins 3 à 4 de ces protéines (HSPA8, KRT10, Rab5A et Rab10). / To complete their life cycle viruses interact with many factors of the host cell. Herpes simplex virus type 1 (HSV-1) is no exception. A recent proteomic study of the virus carried by our laboratory has identified up to 49 cellular proteins potentially incorporated into the mature virions of HSV-1[1]. Since some of these proteins may play important roles during the viral life cycle, they are interesting targets for identification and characterization of new host-pathogen interactions in the context of HSV-1. To target the proteins that are relevant to the viral life cycle of Herpes, the laboratory performed a screening with small interfering RNAs (siRNAs), which showed that at least 15 incorporated proteins are involved in the replication cycle of HSV- 1 in cell culture (Appendix 1). Numerous studies report the incorporation of host proteins in mature virions but few addresses the importance for the viral infectivity of the fractions of cellular proteins incorporated into the virions. To verify this, we depleted these proteins from the mature extracellular virions using siRNAs. Subsequently, we used these viruses to re-infect depleted or normal cells. This method allowed us to identify for the first time eight proteins (DDX3X, HSPA8, KRT10, MIF, Rab5A, Rab6A, Rab10 and 14-3-3ζ) whose absence in virions reduced viral production by at least 50%. As part of understanding at what stage of the life cycle these proteins are necessary for HSV-1, we tested the infectivity of intracellular depleted viruses. Thus, we found at least seven cellular proteins (DDX3X, HSPA8, KRT10, MIF, Rab5A, Rab6A and Rab10) to have a pronounced effect on the replication of herpes virus, which has stimulated us to begin a series of more in-depth tests of the entry of HSV-1. Preliminary results demonstrate the involvement in the entry of HSV-1 of at least three to four proteins (HSPA8, KRT10, Rab5A and Rab10).

O objetivo de colaborar com o equacionamento da problemática do crescimento populacional por meio de uma proposta viável economicamente, e com vantagens tecnológicas com vistas à durabilidade para a armazenagem de gêneros alimentícios em baixas temperaturas motivou o desenvolvimento desta pesquisa, na qual se propõe o uso do concreto como material alternativo para a construção de sistemas de guarda e conservação de alimentos. A partir de um adequado método de dosagem e da tecnologia desenvolvida pelo grupo de pesquisadores do Laboratório de Materiais Avançados à Base de Cimento, foram elaborados dois concretos diferenciados pela incorporação ou não de ar. A durabilidade do concreto para ambientes com baixas temperaturas tem sido relacionada a um sistema de vazios de ar com volume de 6 '+ OU -' 1 % e adequado espaçamento entre as bolhas de ar. O desempenho desses concretos em ambientes com baixas temperaturas foi avaliado acondicionando-os em uma câmara fria com temperatura de - 35 ± 2 graus Celsius. A sanidade dos corpos-de-prova foi monitorada por meio de ensaios não destrutivos (i.e. determinação da freqüência natural). O período de exposição foi de trinta e cinco dias, sendo que após o sétimo dia ocorreu a estabilização dos valores da freqüência natural dos corpos-de-prova. Os dois tipos de concretos ensaiados - com ar incorporado e sem ar incorporado - mostraram-se resistentes nestas condições de ensaio. Os resultados obtidos demonstram que os concretos, de acordo com o método utilizado, atendem a todos os requisitos para os quais foram projetados / The aim to collaborate with the set out of the world population increase problem, using a feasible and economical proposal, with technology advantages, destined to store foodstuff in low temperatures, motivated the development of this research, that suggests concrete as an alternative material to storage and conservation foodstuff system construction. Starting from the concrete design with an adequate method, and based on the technology developed by worker group of Laboratory of Advanced Cement Based Materials, were designed two kinds of concrete: with and without incorporated air. The durability of concrete in low temperatures environment were related to air voids system with 6 '+ OU -' 1% of air content, and to adequate spacing between air voids. The concrete behaviour in low temperatures environment was evaluated placing the specimens in a cold chamber, whose temperatures were about -35 '+ OU -' 2 Celsius degrees. The integrity of the specimens was evaluated by non-destructive method (determination of natural frequency). The exposure period of the specimens was thirty five days, but at seventh day the natural frequencies values were stabilized. Both kind of concrete were resistant in this condition. The obtained results show that concretes, in conformity to the used methodology, performed all requirement for that were designed

This master's thesis deals with the dominion of the Cistercian monastery in Osek. It describes the development of the dominion since the foundation of the monastery till the present day. Its aim is to present the dominion as a distinctive economic and cultural unit interconnected with a system of incorporated parishes and their collatures The thesis is dedicated to 9 incorporated parishes of the Osek monastery that were proved to be part of the dominion during a longer time period. The thesis also explores the issue of the Marian pilgrimage tradition, which was strongly reaffirmed after the Council of Trent. The tradition is described in the context of history of two of the incorporated parishes - Mariánské Radčice and Jeníkov - where it is still possible to find pilgrimage sites with depictions of Virgin Mary as Our Lady of Sorrows.

Pour compléter leur cycle de vie, les virus interagissent avec de nombreux facteurs de la cellule-hôte. Le virus Herpès simplex de type 1 (HSV-1) ne fait pas exception. Une récente étude protéomique du virus effectuée par notre laboratoire a permis d’identifier 49protéines cellulaires potentiellement incorporées dans les virions matures d’HSV-1 [1]. Étant donné que certaines de ces protéines peuvent jouer des rôles importants au cours du cycle de vie du virus, elles constituent des cibles de choix pour identifier et caractériser de nouvelles interactions hôte-pathogène dans le contexte d’HSV-1. D’ailleurs le laboratoire a été effectué un criblage aux petits ARN d’interférence qui a démontré qu'au moins 15 des protéines incorporées sont impliqués dans le cycle de réplication de HSV-1 en culture cellulaire (Annexe 1). Des nombreuses études rapportent l'incorporation des protéines de l'hôte dans les virions matures mais très peu abordent l'importance de la fraction des protéines cellulaires incorporée dans les virions pour le cycle virale. Pour vérifier ça, nous avons déplété ces protéines des virions matures extracellulaires en utilisant des petits ARN d’interférence. Par la suite, nous avons utilisé ces virus déplétés pour réinfecter des cellules déplétées ou normales. Cette méthode nous a permis d'identifier pour la première fois 8 protéines (DDX3X, HSPA8, KRT10, MIF, Rab5A, Rab6A, Rab10 et 14-3-3ζ) dont l'absence dans les virions réduit la production virale d'au moins 50%. Pour mieux comprendre à quelle étape du cycle viral ces protéines sont nécessaires, nous avons aussi quantifié les virus intracellulaires, produits des cellules déplétées individuellement des quinze protéines cellulaires. Ainsi, nous avons trouvé que dans nos conditions 7 de ces 8 protéines cellulaires (DDX3X, HSPA8, KRT10, MIF, Rab5A, Rab6A et Rab10) semblent impliquées dans la production des virus intracellulaires, ce qui nous a stimulés à débuter une série de tests plus approfondis de l’entrée d’HSV-1. Les résultats préliminaires, démontrent l’implication dans l’entrée d’HSV-1 d’au moins 3 à 4 de ces protéines (HSPA8, KRT10, Rab5A et Rab10). / To complete their life cycle viruses interact with many factors of the host cell. Herpes simplex virus type 1 (HSV-1) is no exception. A recent proteomic study of the virus carried by our laboratory has identified up to 49 cellular proteins potentially incorporated into the mature virions of HSV-1[1]. Since some of these proteins may play important roles during the viral life cycle, they are interesting targets for identification and characterization of new host-pathogen interactions in the context of HSV-1. To target the proteins that are relevant to the viral life cycle of Herpes, the laboratory performed a screening with small interfering RNAs (siRNAs), which showed that at least 15 incorporated proteins are involved in the replication cycle of HSV- 1 in cell culture (Appendix 1). Numerous studies report the incorporation of host proteins in mature virions but few addresses the importance for the viral infectivity of the fractions of cellular proteins incorporated into the virions. To verify this, we depleted these proteins from the mature extracellular virions using siRNAs. Subsequently, we used these viruses to re-infect depleted or normal cells. This method allowed us to identify for the first time eight proteins (DDX3X, HSPA8, KRT10, MIF, Rab5A, Rab6A, Rab10 and 14-3-3ζ) whose absence in virions reduced viral production by at least 50%. As part of understanding at what stage of the life cycle these proteins are necessary for HSV-1, we tested the infectivity of intracellular depleted viruses. Thus, we found at least seven cellular proteins (DDX3X, HSPA8, KRT10, MIF, Rab5A, Rab6A and Rab10) to have a pronounced effect on the replication of herpes virus, which has stimulated us to begin a series of more in-depth tests of the entry of HSV-1. Preliminary results demonstrate the involvement in the entry of HSV-1 of at least three to four proteins (HSPA8, KRT10, Rab5A and Rab10).