The aim of the Diploma thesis is to consider an influence of soil management (rotation of crops on arable land and grassland) to quantity and quality of humic substances. These properties are depended not only upon way of soil management, but also upon climatic effects, such are rainfall, temperature, elevation above sea-level, level of underground water and content of clay. Significantly influenced factors are content and form of organic carbon which is very tightly bonded to humic substances. In this work the humic acids content and properties progression was studied in given time period. Also the effect of crop rotations to changes of physical and chemical properties of humic acids in soil environment was observed. Diploma thesis was realized in co-operation with Mendel University in Brno, which supplied elaborating samples. Studied soil samples were taken from humus horizon Cambisol modal Vatín during years 1999–2006, always in autumn. Individual samples were characterized by available analytical methods, which are generally used in humic substances research area. They are especially fluorescence spectrometry, UV-VIS and infrared spectrometry and acid-base and conductometric titrations.

This work is focused on methods of real-time vein imaging using a camera in the near infrared region. The theoretical section describes the dependence of reflectance and absorbance of blood and tissue on the wavelength and suggests appropriate ways of illumination and viewing the bloodstream. The paper describes methods for real-time venous processing and projection. Based on theoretical knowledge a device sensing and projecting an image of real time examination of the venous system and a user-friendly programme for operating this device were created. The created device has been tested on a number of people to confirm proper operation.

This diploma thesis is focused on measurements of nanoparticles infrared absorption. In project is made literar research of sensors in part of IR area, principal design of detector controled by microcontroller for measurement absorption properties of solutions of nanoparticles and microparticles in IR area with diode detector and bank of optical filtres. According to this design of device microcontroller and display device was chosen. This work contains program design for microcontroler and display unit.

This diploma thesis is focused on the design of IP blocks for thermal camera. This termal camera is intended for plant research. The main part of the thermal camera is an infrared detector that detects a frequency in the range of 8 – 14 m. The analogue signal obtained is modified and subsequently digitized. The detector also includes a Peltier device which is designed for both detector cooling and heating, and is controlled by an external signal. The MicroZed development board implements IP blocks. The thermal camera is designed to be controlled from the MicroZed board. Device analysis is first described from the theoretical part to the design of IP blocks.

Le traitement des infections intracellulaires est compliqué par la capacité des bactéries à «se cacher» à l’intérieur des cellules de l’hôte, en particulier celles du système immunitaire, entravant ainsi l’action de nombreux agents antimicrobiens. La diffusion croissante de souches résistantes est très inquiétante. Dans ce cadre, les nanoparticules (NPs) constituent une stratégie prometteuse pour administrer de manière optimisée des agents antimicrobiens.Ce travail de thèse, réalisé dans le cadre du projet européen ITN Cyclon Hit, visait à développer et caractériser des NPs biodégradables et biocompatibles chargées en antibiotiques, composés d’acide polylactique (PLA), d’acide poly (lactique-co-glycolique) (PLGA) et de polycaprolactone (PCL) ou de cyclodextrines polymérisées (pCD).Les deux premiers chapitres sont consacrés aux verrous technologiques liés à l'encapsulation de certains médicaments puissants dans les NPs polymériques. Tout d'abord, ces vecteurs ont été utilisés pour la délivrance simultanée d'une combinaison de molécules actives récemment découverte, l'éthionamide (ETH) et son Booster, pour le traitement de la tuberculose. Deuxièmement, ils ont été employés pour relever les défis liés à l'incorporation d'une quinolone de première génération, l'acide pipémidique (PIP), dans le but d'optimiser sa distribution intracellulaire dans des infections telles que la salmonellose.La co-incorporation efficace de l'ETH et du booster a dû surmonter de nombreuses difficultés liées à des problèmes de solubilité, de cristallisation et de biodisponibilité. Nos NPs en PLA et en pCD ont montré leur capacité de co-encapsuler efficacement les deux molécules et tout particulièrement celles en pCD. Elles incorporent les médicaments à la fois dans les cavités des CD et dans des microdomaines hydrophobes. Les NPs en pCD, non toxiques après administration pulmonaire répétée de fortes doses, ont été administrés in vivo par voie endotrachéale directement au site d'infection. Elles ont permis une diminution de 3-log de la charge bactérienne pulmonaire des animaux infectés après seulement 6 administrations. De même, l'incorporation de PIP a été confrontée à des défis liés à la cristallisation de PIP et à sa libération incontrôlée. Malheureusement, le PIP présentait une faible affinité pour tous les matériaux polymériques étudiés et son encapsulation physique était infructueuse. Ainsi, une approche alternative a été développée en couplant le PIP au PCL via une réaction sans catalyseur initiée par le médicament. Le conjugué PCL-PIP se auto-assemble en forme de NPs avec une charge en PIP de 27%. Cependant, le PCL-PIP n'a pas pu être dégradé in vitro, mais l’approche de synthèse de conjugués est séduisante pour obtenir de particules stables et avec un contenu important en PIP.La compréhension approfondie de la structure et de la composition du noyau et de la couronne des nanostructures contenant une ou deux molécules actives est cruciale pour leur optimisation. Les deux derniers chapitres sont donc consacrés à l'application innovante de l'AFM-IR, une méthode nanospectroscopique originale combinant la microscopie à force atomique (AFM) avec la spectroscopie infrarouge (IR), à l'analyse chimique des NPs en PLGA ou à leur détection sans marquage après internalisation dans les cellules.L’AFM-IR est capable de fournir une caractérisation chimique à l'échelle nanométrique (résolution ~10 nm). Une avancée majeure du travail est l'application du mode tapping permettant l'investigation individuelle de chaque NP. Le signal IR spécifique des composants des NPs a été utilisé pour appréhender la composition chimique de leur cœur et couronne ainsi que pour localiser précisément le médicament. De plus, l'AFM-IR en mode contact a permis pour la première fois la localisation sans marquage et l'identification chimique des NP à l'intérieur des cellules. Ce travail ouvre la voie à d'innombrables applications de cette technique dans le domaine de la nanomedecine. / The treatment of intracellular infections is very challenging given the ability of bacteria to “hide” inside the cells of the host, especially the ones of the immune system, thus hampering the action of many antimicrobial agents. The battle against these bacteria has been further exacerbated by the increasing diffusion of antimicrobial resistant strains. In this frame, nanoparticles (NPs) are a very promising strategy to overcome the limitations of free antimicrobial agents by administering them in an optimized manner.This PhD work, performed as part of the European Project ITN Cyclon Hit, aimed at the development and advanced characterisation of antibiotic-loaded biodegradable and biocompatible NPs made of poly (lactic acid) (PLA), poly (lactic-co-glycolic) (PLGA) and polycaprolactone (PCL) or of polymerised cyclodextrins (pCDs).The first two chapters are dedicated to the encapsulation of powerful but challenging drugs in polymeric NPs. Firstly, these carriers were employed for the simultaneous delivery of a potent drug combination recently discovered, ethionamide (ETH) and its booster, for tuberculosis therapy. Secondly, they were used to address the challenges related to the incorporation of a first-generation quinolone, pipemidic acid (PIP), with the aim of optimising its intracellular delivery in infections such as salmonellosis.The efficient co-incorporation of ETH and booster had to overcome several technological barriers. These drugs presented solubility, crystallisation and bioavailability-related problems which were overcome thanks to the developed NPs. Our engineered PLA and pCD NPs were both able to efficiently co-encapsulate the two molecules. Among the in depth-characterised formulations, pCDs NPs displayed the best physico-chemical properties and were shown to host the drugs both in the CD cavities and in confined spaces inside NPs crosslinked polymer. The pCD NPs were administered in vivo by endotracheal route directly to the infection site. Empty NPs were shown non-toxic after repeated pulmonary administration of high doses. Moreover, loaded pCD NPs led to a 3-log decrease in the pulmonary bacterial load of infected animals after only 6 administrations. Similarly, the incorporation of PIP faced challenges mainly related to PIP crystallization and burst release. Unfortunately, PIP displayed poor affinity for all the studied polymeric materials and its physical encapsulation was unsuccessful. Thus, an alternative approach was developed by coupling PIP to PCL by using an original catalyst-free drug-initiated reaction. The PCL-PIP conjugate self-assembled in NPs with up to 27 wt% PIP which were thoroughly characterised. However, the conjugate couldn’t be enzymatically degraded. With the design of novel PCL-PIP conjugates, this self-assembly approach could represent a promising strategy.The deep understanding of the structure and composition of complex core-corona nanocarriers containing one or two active molecules is crucial for their optimisation. The last two chapters are devoted to the innovative application of AFM-IR, an original nanospectroscopic method combining atomic force microscopy (AFM) with infrared (IR) spectroscopy, to the chemical analysis of PLGA NPs or to their label-free detection after cell internalisation.AFM-IR is able to provide chemical characterisation at the nanometer scale (resolution ~10nm). One main breakthrough here is the application of the recently developed tapping mode allowing the investigation of single polymeric NPs. The specific IR signal of NPs constituents was used to unravel the chemical composition of their core and corona as well as to precisely locate the drug. Moreover, the AFM-IR in contact mode enabled for the first time the label-free localisation and unambiguous chemical identification of NPs inside cells using the polymer IR specific response as a fingerprint. This work paves the way for countless application of this technique in the field of drug delivery.

Loess deposits are terrestrial clastic sediments derived from aeolian deposition. Loess is transported in periglacial conditions and was mainly deposited in cold environments around glaciers during the Quaternary period. To be able to understand and interpret past climate and how it was changing, loess deposits can be used as an indicator for example wind circulation and dustiness in the atmosphere. However, good independent chronological control is essential for using loess as a past climate archive. This study has examined loess from Lowland Point in the southwest of England. The loess at Lowland Point is relatively thin at 180 cm compared with more studied loess deposits further east on continental Europe. To be able to date the k-feldspar mineral grains from the loess deposit at Lowland Point, two values should be constrained for each sample: the equivalent dose (DE), a quantitative factor of the amount of energy accumulated by the mineral grain during burial from ionising radiation, and the dose rate (DR), a rate of ionising radiation absorbed from the surrounding environment per year. By using the post infrared-infrared stimulated luminescence (post-IR IRSL) method, the DE values were obtained, and by examine radioactive isotopes in the samples, the DR values were attained. The results of each average age per sample depth was then identified and the loess deposit could be determined to the age about 10.8-22.4 ka, which is suggested to have been deposited by meltwater from the British Irish Ice Sheet and permafrost. / Lössjordar är avlagringar av markbundna klastiska sediment härledda från eolisk deposition och transporteras under periglaciala förhållanden för att huvudsakligen deponeras i kalla miljöer runt glaciärer under kvartärperioden. För att kunna förstå och tolka tidigare klimat och hur det förändrades kan lössavlagringar användas som en indikation över till exempel vindcirkulation och dammighet i atmosfären. Denna studie har undersökt löss från Lowland Point i sydvästra England. Lössen vid Lowland Point är relativt tunn, med en tjocklek på 180 cm, jämfört med mer studerade lössjordar. För att kunna datera kalifältspat från lössjorden vid Lowland Point var förhållandet mellan två komponenter av betydelse; ekvivalentdosen (DE), en kvantitativ faktor för den mängd energi som mineralkornet ackumulerar vid sedimentering, och doshastigheten (DR), en hastighet av den mängd energi som absorberas i den omgivande miljön per år. Genom att använda metoden post infraröd-infraröd stimulerad luminescens (post-IR IRSL) erhölls DE-värden och genom att undersöka radioaktiva isotoper i proverna hittades DR-värden. Resultaten av varje medelålder per provdjup identifierades sedan och lössavlagringen kunde bestämmas till en ålder av cirka 10,8–22,4 ka, vilket indikerar en möjlig avsättning av smältvatten från det brittiska irländska istäcket och permafrost.

The power and energy optimization of a device can lead to a reduced cost, smaller area, better temperature performance, and higher lifetime. Furthermore, in systems that have limited power budget, it allows running simultaneously more functionalities or using features that require higher power demand.Therefore, both from the user and the company perspective, the value of a product increases as the energy optimization improves. For nighttime surveillance video recording, it is common to use infrared illumination to light the targetscene, which draws a significant portion of the total camera energy consumption. This master thesis examines and discusses how stroboscopic infrared illumination can enhance the energy efficiency in videorecording cameras with rolling shutter image sensors. This report analyzes LED driver circuits, recommends methodologies, and sorts the most relevant parameters to help to dimension and design the illumination system for a light-strobing system. A promising field of use for this technique has been found to be the license-plate recognition (LPR) scenario, for which this thesis dedicates a chapter in this document. This project has been developed at AXIS Communications, where a prototype has been built for one of their network security cameras. The prototype has been tested for LPR for both strobing light systems and conventional IR lighting systems. The results obtained prove that the energy efficiency of the illumination system can be improved more than 95% when stroboscopic illumination is used. / Effektförbrukning och energioptimering av en produkt kan leda till lägre kostnad, mindre storlek, bättre temperaturprestanda och högre livslängd. I system med begränsad effektbudget möjliggör detta dessutom aktivering av fler funktioner samtidigt, eller användning av funktioner med högre strömförbrukning. Därmed gör energioptimering att produktens värde ökar både för användaren och för företaget som tillverkar den. För videoinspelning med övervakningskamera nattetid är det vanligt att använda infraröd belysning för att belysa scenen, vilket ofta förbrukar en betydande del av kamerans totala effektbudget. Detta examensarbete undersöker och diskuterar hur blixtrande (Eng. strobed) infraröd belysning kan förbättra energieffektiviteten vid videoinspelning med bildsensorer med rullande slutare. I denna rapport analyseras LED-drivkretsarna, metodik rekommenderas samt att de mest relevanta parametrarna för att dimensionera och designa ett belysningssystem baserad på strobed IR-belysning sorteras ut. Ett lovande användningsområde för denna teknik har visat sig vara LPR-scenariot (License Plate Recognition), vilket diskuteras i ett eget kapitel i denna rapport. Projektet har genomförts på AXIS Communications, där en prototyp har byggts baserat på en av dess nätverkskameror. Prototypen har utvärderats LPR-sammanhang med både strobed och konventionellt IR belysningssystem. De erhållna resultaten visar att energieffektiviteten hos belysningssystemet kan förbättras med mer än 95% när blixtrande belysning används.

The oceans are an essential global resource for all living organisms but especially for us humans. However, year after year we continue to neglect proper recycling of our waste, resulting in litter ending up in our oceans. The majority of said litter comes from single use plastic items. Through fragmentation and erosion, the plastic dissolves  to smaller pieces, once they are no larger than 5 mm theyare classified as micro- and nanoplastics. Little is known about these small plastic particles impact on marine life and marine environment. As a step towards understanding this, the robot PlaCo was created. PlaCo stands for plastic collecting which is exactly what the robot does. With the help of three filters PlaCo gathers marine debris, such as plastic, from the water in which it operates. The filters have decreasing mesh size resulting in the microplastics being caught in the last one. Once emptied, the finds can be examined and logged for future referencing. With the help of a sensor, blockages of the filters can be monitored and if detected, notice will be given to the user through a LED. In order for PlaCo to move forward and for water to travel through the filters, the robot was provided with two DC motors. A microcontroller, Arduino Uno, was used to regulate PlaCo’s functions. The performance of two different sensors, an IR-sensorand an ultrasonic sensor, were investigated as well as the robot's water cleaning capacity. The results indicate that the latter of the two sensors would be preferable due to its high reliability. However, the robot’s water cleaning capacity could not be measured due to the chosen motors not being powerful enough. In future iterations of PlaCo, thiswould need to be rectified. / Globalt sett är världens hav en viktig resurs för alla levande organismer men inte minst för människan. Trots detta fortsätter vi att, år efter år, försumma återvinningen av vårt avfall vilket resulterar i att skräp i stället hamnar i haven. Majoriteten av de sopor som hamnar där är resultatet av förbrukade engångsprodukter i plast. Genom sönderfall och erosion skapas allt mindre och mindre bitar av plast. Detta resulterar i att så kallade mikro- och nanoplaster skapas. De är mindre än 5 mm i bredd och om deras påverkan på det marina djurlivet och den marina miljön vet vi mycket lite om. För att minska kunskapsluckorna och för att få en bättre förståelse för deras påverkan har nu därför PlaCo konstruerats. Med hjälp av tre sorters filter kan PlaCo samla upp marintskräp, så som plast, i vattnet där den arbetar. Filtrens finhet varierar, där det första är mycket grovt medan det sista är fint nog att klara av att samla upp mikroplaster. När PlaCo sedan töms kan mikroplasterna undersökas och dokumenteras. När en tömning behöver göras indikeras detta för användaren med hjälp utav en LED. Roboten är försedd med en sensor som läser av hur fulla filtren är. För att driva PlaCo framåt och för att underlätta filtreringsprocessen är den också försedd med två 6 V DC-motorer. Allt detta styrs med hjälp av mikrokontrollern Arduino Uno. För att uppnå bästa tänkbara funktion hos roboten undersöktes två olika sensortyper, en IR-sensor och en ultraljudssensor. Det visade sig att ultraljudssensorn var betydligt mer pålitlig än IR-sensorn och därför valde man att använda denna. Det var även av intresse att ta reda på hur mycket vatten PlaCo kunde rena per sekund. Tyvärr skulle det visa sig att de valda motorerna inte var kraftfulla nog att driva PlaCo i vattnet. Det är därför något som behöver åtgärdas i en framtida version av PlaCo.

Chimie organométallique résonne avec catalyse homogène, et chimie des surfaces avec catalyse hétérogène. Mais la frontière établie entre ces deux domaines est en réalité très mince. Leur rapprochement aboutit dans les années 1990 au développement d’une nouvelle science : la chimie organométallique de surface, qui souligne leur complémentarité. L’objectif de cette science, dans laquelle s’inscrit ce travail de thèse, est de créer des catalyseurs hétérogènes à partir de composés organométalliques. Notre objectif est d’apporter une contribution à la compréhension de la formation de nanoparticules de platine - métal incontournable en catalyse hétérogène - sur des supports de silice amorphe, et ce grâce à la chimie organométallique. Au cours de ce travail, une palette de catalyseurs Pt/SiO2 a été préparée à partir de trois précurseurs de platine : le composé classique H2PtIVCl6.xH2O et deux composés organométalliques PtII(η 4-C8H12)Cl2 et Pt0(η 2-C7H10)3 , et de trois supports : une silice commerciale (Davison), une silice mésoporeuse SBA-15 synthétisée au laboratoire et unesilice naturelle, la diatomite. De plus, l’étude du catalyseur de référence au platine « EuroPt-1 » a permis de développer une méthodologie de suivi operando par Spectroscopie Infrarouge à Transformée de Fourier par Réflexion Diffuse (DRIFTS) de l’adsorption du monoxyde de carbone (CO) sur les différents sites d’une nanoparticule de platine. En conclusion, les catalyseurs préparés à partir de Pt0(η 2-C7H10)3 s’avèrent être plus actifs qu’EuroPt-1 pour la réaction d’oxydation du CO, ce qui démontre le potentiel d’utilisation des composés organométalliques dans le domaine de la catalyse hétérogène. / Organometallic chemistry resonates with homogeneous catalysis, and surface chemistry with heterogeneous catalysis. But the frontier between these two fields is very thin. In the 90’s, these fields approached each other and led to the development of a new science: organometallic surface chemistry, which underlines their complementarity. The goal of this science, with which this work is associated, is to create heterogeneous catalysts from organometallic compounds. Our goal is to contribute to the understanding of platinum nanoparticle formation - platinum being an important metal in heterogeneous catalysis - onto amorphous silica supports, thanks to organometallic chemistry. During this work, various Pt/SiO2 catalysts were prepared fromthree platinum precursors: the classical one, H2PtIVCl6.xH2O and two organometallic compounds PtII(η 4-C8H12)Cl2 et Pt0(η2-C7H10)3, and involved three supports: a commercial silica (Davison), a mesoporous silica SBA-15 synthesized in the laboratory, and a natural silica, the diatomite. Moreover, the study of the standard platinum reference catalyst “EuroPt-1” lead to the development of a methodology of operando Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS) of carbon monoxide (CO) adsorption on the different sites of a platinum nanoparticle. Catalysts prepared from Pt0(η 2-C7H10)3 are more active than EuroPt-1 for the CO oxidation reaction, and this work shows the potential of organometallic precursors in the domain of heterogeneous catalysis.

Das Thema Information Retrieval hat insbesondere in Form von Internetsuchmaschinen eine große Bedeutung erlangt. Retrievalsysteme werden für eine Vielzahl unterschiedlicher Rechercheszenarien eingesetzt, unter anderem für firmeninterne Supportdatenbanken, aber auch für die Organisation persönlicher E-Mails. Eine aktuelle Herausforderung besteht in der Bestimmung und Vorhersage der Leistungsfähigkeit einzelner Komponenten dieser Retrievalsysteme, insbesondere der komplexen Wechselwirkungen zwischen ihnen. Für die Implementierung und Konfiguration der Retrievalsysteme und der Retrievalkomponenten werden Fachleute benötigt. Mithilfe der webbasierten Lernanwendung Xtrieval Web Lab können Studierende praktisches Wissen über den Information Retrieval Prozess erwerben, indem sie Retrievalkomponenten zu einem Retrievalsystem zusammenstellen und evaluieren, ohne dafür eine Programmiersprache einsetzen zu müssen. Spielemechaniken leiten die Studierenden bei ihrem Entdeckungsprozess an, motivieren sie und verhindern eine Informationsüberladung durch eine Aufteilung der Lerninhalte. / Information retrieval has achieved great significance in form of search engines for the Internet. Retrieval systems are used in a variety of research scenarios, including corporate support databases, but also for the organization of personal emails. A current challenge is to determine and predict the performance of individual components of these retrieval systems, in particular the complex interactions between them. For the implementation and configuration of retrieval systems and retrieval components professionals are needed. By using the web-based learning application Xtrieval Web Lab students can gain practical knowledge about the information retrieval process by arranging retrieval components in a retrieval system and their evaluation without using a programming language. Game mechanics guide the students in their discovery process, motivate them and prevent information overload by a partition of the learning content.