The multi-million-dollar medical uniform industry has not utilized advancements in garment and textile technology that could positively impact the protection of healthcare professionals and patients. In most cases the uniforms meet basic requirements – they clothe the professional in a recognizable way. Little innovation in design, function and performance, has been applied to these garments. This is particularly evident in the case of the stereotypical white lab coat worn by many physicians, despite evidence indicating that these lab coats may carry contamination and play a role in the spread of deadly bacteria. Healthcare Associated Infections (HAIs) are among the most serious problems facing modern medical care, costing millions of lives and dollars annually worldwide. This research investigates the design and use of the physician’s lab coat – an immediately recognizable symbol of Western medicine. The research identifies the medical, functional, cultural and symbolic roles of the lab coat within the hospital environment and beyond, to the larger the global society. This thesis examines the extent to which the design of medical wear can impact the effect of hospital-acquired infections, support doctor/patient relationships and enhance the performance and behavior of the healthcare professional by envisioning a future lab coat which offers increased protection for physician and patient, aids in communication and enhances the performance of the doctor by utilizing digital technologies incorporated into the lab coat whereby the lab coat becomes the only tool necessary for the physician.

The industry is facing major challenges today. The challenges are tougher global competition, customers who require individualized products and shorter product lifecycles. The predicted industrial revolution is a way to deal with these challenges. Industry 4.0 includes strategies linked to several technologies that will meet the new needs. Smart factory is a central concept in industry 4.0, which involves connected technologies of various kinds. Such as digital manufacturing technology, network communication technology, computer technology, automation technology and several other areas. In this work, these were defined as smart factory technologies. Implementing such technologies will result in improved flexibility, resource productivity and efficiency, quality, etc. But, implementing smart factory technologies poses major challenges for the companies. Laboratory environments can be utilized to address the challenges. This results in a new problem, how to transfer a smart factory technology developed in a laboratory environment to a full-scale production system. In the literature study no, structured approach was identified to handle this challenge. Therefore, the purpose of this work was to: create a work process that supports the technology transfer from a smart factory compliant laboratory environment to a full-scale production system. To justify the purpose, the following research questions were answered: RQ1: What are the differences in the operating environment between the laboratory and the full-scale production system? RQ2: How is a smart factory technology determined ready to be implemented into a full-scale production system? RQ3: What critical factors should a work process for the implementation of smart factory technologies include? The research questions were answered by conducting a multiple-case study in collaboration with Scania CV AB. During the case studies, interviews, observations and other relevant types of data collection were conducted. The results were as follows: RQ1: How difficult it is to transfer a technology from a laboratory environment to a full-scale production system depends on how large the differences between these are. The general difference is that laboratory environments are used to experiment and develop technologies and a full-scale production system is used to produce products. Some want the laboratory environment to be an exact copy of a full-scale production system, but this is not appropriate because it means you lose the freedom of experimentation and it would be much more expensive. RQ2: Determining whether a smart factory technology is ready consists of two parts, laboratory activities and pilot testing. A structured assessment method has been developed. The laboratory operations reduce the risks and contribute to raising the degree of maturity of the technology. In pilot testing, it is important not to interfere with the full-scale production system stability. This is the reason for doing pilot testing in a delimited area first and checking that the technology works as desired. RQ3: The critical factors identified were: competence and knowledge, technology contributing to improvements, considering risks with implementation, cost versus potential improvement, clear goals and reason for implementation and communication.

Die nur durchschnittlichen Ergebnisse vor allem in den Naturwissenschaften deutscher Schüler in internationalen Vergleichsstudien motivierten vielfältige Initiativen zur Gründung außerschulischer Lernorte. Diese haben u. a. das Ziel, dem schwindenden Interesse z. B. an Physik entgegenzutreten und so neben der Schule einen Beitrag zur naturwissenschaftlichen Grundbildung der Schüler zu leisten. Eine Variante dieser Lernorte sind so genannte Schülerlabore, in denen Schüler die Möglichkeit haben, sich intensiv mit naturwissenschaftlichen Fragestellungen zu beschäftigen und selbständig Experimente durchzuführen. Wenig erforscht ist jedoch die Wirksamkeit dieser Einrichtungen bezogen auf kognitive und affektive Ziele. Die vorliegende explorative Arbeit geht der Frage nach, wie sich das Interesse von Schülern der fünften und achten Jahrgangsstufe an Physik bei dreimaligen Besuchen eines Schülerlabors innerhalb eines Schulhalbjahres entwickelt. Zusätzlich ergründet sie am Beispiel der Anfangsoptik, wie sich eine von fachdidaktischer Seite geforderte Einbindung der Besuche in das laufende Curriculum auf die Interessenentwicklung auswirkt. Die Ergebnisse der auf Fragebogenerhebungen gestützten Studie lassen die Vermutung zu, dass Besuche eines Schülerlabors altersübergreifend lediglich kurzfristig positive Effekte hervorrufen. Das durch den Besuch gesteigerte Interesse geht bereits innerhalb weniger Wochen wieder verloren. Ein anfängliches Interessenniveau kann nur durch einen erneuten Besuch annähernd wieder erreicht werden. Die Besuche können somit nur den aus der psychologischen Forschung bekannten „Catch“-Faktoren zugeordnet werden. Diese können zwar als wirksames Mittel dienen, kurzfristig das Interesse zu wecken, sie sind jedoch nicht imstande, dieses mittelfristig aufrecht zu halten. Für eine Genese langfristig wirkender Interessen sind so genannte „Hold“-Komponenten erforderlich, die für eine Stabilisierung sorgen. Die vorliegende Arbeit gibt Hinweise darauf, dass dies durch eine thematische und methodische Einbindung der Besuche in das laufende Curriculum gewährleistet werden kann. Lehrer und Betreiber von außerschulischen Lernorten sollten dies daher zum Anlass nehmen, ihre Bildungsabsichten stärker miteinander abzustimmen. / International assessment studies revealed mediocre results of german students especially in science and thus motivated several initiatives to establish extracurricular learning facilities. Among others these facilities aspire to overcome the decreasing interest as for instance in physics and to contribute to students’ scientific literacy. One type of these informal learning settings are so-called science labs for school students. There, students have the chance to occupy themselves with scientific problems and to conduct experiments autonomously. Research concerning the effectiveness of these facilities regarding both cognitive and affective aims is scarce though. The present explorative study examines the impact of three visits to a science lab for school students on the development of interest in physics of 5th- and 8th-grade students. In addition, taking optics as an example it investigates the effectiveness of an integration of the visits into the current school curriculum as demanded frequently. Surveyed by questionnaires the results of the study give rise to the assumption that visits to a science lab cause positive effects only on a short time scale for all investigated age groups. The increased interest immediately after the visit declines within a few weeks. An initial level can only be reached by a subsequent visit. Hence, visits can only be assigned as “catch”-facets as known in psychological research. Therefore they can serve as an effective means to arouse interest but they are not capable of sustaining it on a mid term time scale. The genesis of long term interest requires so called “hold”-facets, which in turn stabilise the interest. The present study indicates that this stabilisation can be obtained by a thematic and methodical integration of the visits into the ongoing curriculum. Thus, teachers and carriers of extracurricular learning facilities should strive for a coordination of their educational objectives.

The rapid development of optofluidics, the combination of microfluidics and integrated optics, since its formal conception in the early 2000's has aided in the advance of single-molecule analysis. The optofluidic platform discussed in this dissertation is called the liquid core anti-resonant reflecting optical waveguide (LC-ARROW). This platform uses ARROW waveguides to orthogonally intersect a liquid core waveguide with solid core rib waveguides for the excitation of specifically labeled molecules and collection of fluorescence signal. Since conception, the LC-ARROW platform has demonstrated its effectiveness as a lab-on-a-chip fluorescence biosensor. However, until the addition of optical multiplexing excitation waveguides, the platform lacked a critical functionality for use in rapid disease diagnostics, namely the ability to simultaneously detect different types of molecules and particles. In disease diagnostics, the ability to multiplex, detect and identify multiple biomarkers simultaneously is paramount for a sensor to be used as a rapid diagnostic system. This work brings optofluidic multiplexing to the sensor through the implementation of three specific designs: (1) the Y-splitter was the first multi-spot excitation design implemented on the platform, although it did not have the ability to multiplex it served as a critical stepping stone and showed that multi-spot excitation could improve the signal-to-noise ratio of the platform by ~50,000 times; (2) a multimode interference (MMI) waveguide which took the multi-spot idea and then demonstrated spectral multiplexing capable of correctly identifying multiple diverse biomarkers simultaneously; and, (3) a Triple-Core design which incorporates excitation and collection along multiple liquid cores, enabling spatial multiplexing which increases the number of individual molecules to be identified concurrently with the MMI waveguide excitation. In addition to describing the development of optical multiplexing, this dissertation includes an investigation of another LC-ARROW based design that enables 2D bioparticle trapping, the Anti-Brownian Electrokinetic (ABEL) trap. This design demonstrates two-dimensional compensation of a particle's Brownian motion in solution. The capability to maintain a molecule suspended in solution over time enables the ability to gain a deeper understanding of cellular function and therapies based on molecular functions.

<p>The objective of this thesis is to design and implement a small scaleInternet Service Provider (ISP) for the NetCenter sub department atMälardalen University. The ISP is intended to give NetCenter a networkseparate from the University’s network, providing them with a moreflexible environment for lab purposes. This will give their students anopportunity to experience a larger backbone with Internet accessibility,which has not been previously available. At the same time it will place theteachers in control of the network in the NetCenter lab premises.The network is designed with a layered approach including an Internetaccess layer, a larger core segment and a distribution layer with aseparated lab network. It also incorporates both a public and a privateserver network, housing servers running e.g. Windows Active Directory,external DNS services, monitoring tools and logging applications. TheInternet access is achieved by peering with SUNET providing a full BGPfeed.This thesis report presents methods, implementations and results involvedin successfully creating the NetCenter ISP as both a lab network and anInternet provider with a few inevitable shortcomings; the most prominentbeing an incomplete Windows Domain setup.</p>

<p>There is a strong trend in fabricating <i>miniaturized total analytical systems</i>, µTAS, for various biochemical and cell biology applications. These miniaturized systems could e.g. gain better separation performances, be faster, consume less expensive reagents and be used for studies that are difficult to access in the macro world. Disposable µTAS eliminate the risk of carry-over and can be fabricated to a low cost.</p><p>This work focused on the development of µTAS modules with the intentional use for miniaturized diagnostics. Modules for blood separation, desalting, enrichment, separation and ESI-MS detection were successfully fabricated. Surface coatings were additionally developed and evaluated for applications in µTAS with complex biological samples. The first heparin coating could be easily immobilized in a one-step-process, whereas the second heparin coating was aimed to form a hydrophilic surface that was able to draw blood or plasma samples into a microfluidic system by capillary forces. </p><p>The last mentioned heparin surface was further utilized when developing a chip-based sensor for performing CD4-count in human blood, an important marker to determine the stage of an HIV-infection.</p><p>All devices in this work were fabricated in PDMS, an elastomeric polymer with the advantage of rapid and less expensive prototyping of the microfabricated master. It was shown that PDMS could be considered as the material of choice for future commercial µTAS. The devices were intentionally produced using a low grade of fabrication complexity. It was however demonstrated that even with low complexity, it is possible to integrate several functional chip modules into a single microfluidic device.</p>

There is a strong trend in fabricating miniaturized total analytical systems, µTAS, for various biochemical and cell biology applications. These miniaturized systems could e.g. gain better separation performances, be faster, consume less expensive reagents and be used for studies that are difficult to access in the macro world. Disposable µTAS eliminate the risk of carry-over and can be fabricated to a low cost. This work focused on the development of µTAS modules with the intentional use for miniaturized diagnostics. Modules for blood separation, desalting, enrichment, separation and ESI-MS detection were successfully fabricated. Surface coatings were additionally developed and evaluated for applications in µTAS with complex biological samples. The first heparin coating could be easily immobilized in a one-step-process, whereas the second heparin coating was aimed to form a hydrophilic surface that was able to draw blood or plasma samples into a microfluidic system by capillary forces. The last mentioned heparin surface was further utilized when developing a chip-based sensor for performing CD4-count in human blood, an important marker to determine the stage of an HIV-infection. All devices in this work were fabricated in PDMS, an elastomeric polymer with the advantage of rapid and less expensive prototyping of the microfabricated master. It was shown that PDMS could be considered as the material of choice for future commercial µTAS. The devices were intentionally produced using a low grade of fabrication complexity. It was however demonstrated that even with low complexity, it is possible to integrate several functional chip modules into a single microfluidic device.

Laboratory work is considered important for student achievements in science education. This thesis will contribute with increased knowledge about lab work in science education in Swedish secondary school. The main purposes are to describe secondary school science teachers’ objectives for lab work and to describe how these objectives are implemented during laboratory exercises. The thesis shows and discusses, from a teacher perspective, the complexity involved in lab work.The thesis is comprised of four papers based on empirical analysis of teacher interviews, laboratory manuals and laboratory exercises. Two interview studies identified which objectives the teachers consider important and compared these to international studies. Two case studies identified how the teachers’ objectives are put forward during lab work and what factors are important for the implementation of objectives.The results from the interview studies show that Swedish secondary school science teachers express general objectives including the development of students’ understanding of concepts and phenomena, of their interest in science and ability to think and reflect upon labwork. This is to a large extent in accordance with objectives identified in international studies. However, when the teachers describe specific laboratory exercises they emphasize the activity and the laboratory skills. Some of the teachers describe lab work that includes scientific inquiry but not specifically, knowledge about the nature of science. Scientific inquiry was mostly used to develop interest in science and not to develop knowledge about how to systematically investigate phenomena in nature. The teachers express their objectives differently in different contexts. The laboratory manuals mostly put forward objectives to help students identify objects and phenomena and to learn facts, which is not always in accordance with the teachers objectives. Results from the case studies show that the teachers’ objectives do not always correspond to the students’ views of important things to learn. It is not obvious that lab work in itself make students understand a certain scientific content, they need help to “see what is intended to be seen”. Interactions between the teacher and the students are important to help students perceive the teacher’s objectives. Many interactions have a starting point in the laboratory manuals, and if the objectives in the manual correspond to the teacher’s objectives it makes it easier for both the students and the teacher to reach the intentions for the laboratory exercise. Implications for science teaching are discussed. / Att laborationer har en naturlig och central plats i naturvetenskaplig undervisning håller de flesta med om men hur stor vikt svenska grundskollärare lägger på det laborativa arbetet och dess betydelse för elevers lärande i naturvetenskap är inte klarlagt. Denna avhandling ska ge ytterligare kunskap om det laborativa arbetet i svensk grundskola. Avhandlingen har två huvudsyften. Det ena är att ge en beskrivning av de mål för laborativt arbete som lärare i den svenska grundskolans senare år anser viktiga. Det andra är att beskriva hur laborationer som genomförs i skolpraktiken förverkligar de uppsatta målen. Avhandlingen uppmärksammar och diskuterar det laborativa arbetets komplexitet utifrån ett lärarperspektiv.De fyra delstudierna bygger på empiriska undersökningar av intervjuer med lärare, deras laborationsinstruktioner och av det laborativa arbetets genomförande. I två intervjustudier analyseras vilka mål som anses viktiga och hur dessa förhåller sig till internationell forskning om mål med laborationer. I två fallstudier analyseras hur lärarens mål framträder under det laborativa arbetet och vilka faktorer som har betydelse för hur målen implementeras.Resultaten från intervjustudierna visar bland annat att lärare i den svenskagrundskolan uttrycker generella mål för laborativt arbete som att eleverna skautveckla sin förståelse av naturvetenskapliga begrepp och fenomen, sitt intresse för naturvetenskap, och sitt reflekterande över laborativt arbete. Detta överensstämmer i stor utsträckning med mål som framträder i internationella undersökningar. När lärarna talar om specifika laborationer betonar de istället själva aktiviteten och de laborativa färdigheterna. Lärarna uttrycker således sina mål olika i olika sammanhang. Lärarna erbjuder laborationer där undersökande arbete förekommer men de utnyttjar inte laborationerna till att skapa förståelse av naturvetenskapens karaktär. Det undersökande arbetet utnyttjas främst för att öka intresset för naturvetenskap och inte för att ge kunskap om metoder för naturvetenskapliga undersökningar. Laborationsinstruktionerna innehåller i stor utsträckning mål för att hjälpa elever att identifiera objekt och att lära sig fakta. Instruktionernas mål stämmer inte alltid överens med lärarnas mål med laborationerna. Resultaten från fallstudierna visar att lärarna ofta har fler mål med laborationerna än de som kommer fram under genomförandet och att lärarnas mål inte alltid överensstämmer med vad eleverna uppfattar som viktigt. Det är inte självklart att det laborativa arbetet i sig medför att eleverna förstår ett visst naturvetenskapligt innehåll, eleverna behöver hjälp att ”se vad som är avsett att se”. Interaktionerna mellan lärare och elever och mellan elever och elever är mycket viktiga för att eleverna ska uppfatta målen. Mycket av interaktionerna tar sin utgångspunkt i laborationsinstruktionen. Om målen i denna överensstämmer med de mål läraren vill eftersträva underlättar det både för läraren och för eleverna. I avhandlingen diskuteras konsekvenser för undervisningen.

The field of micro total analysis systems (μTAS) aims at developments toward miniaturized and fully integrated lab-on-a-chip systems for applications, such as drug screening, drug delivery, cellular assays, protein analysis, genomic analysis and handheld point-of-care diagnostics. Such systems offer to dramatically reduce liquid sample and reagent quantities, increase sensitivity as well as speed of analysis and facilitate portable systems via the integration of components such as pumps, valves, mixers, separation units, reactors and detectors. Precise microfluidic control for such systems has long been considered one of the most difficult technical barriers due to integration of on-chip fluidic handling components and complicated off-chip liquid control as well as fluidic interconnections. Actuation principles and materials with the advantages of low cost, easy fabrication, easy integration, high reliability, and compact size are required to promote the development of such systems. Within this thesis, liquid displacement in microfluidic applications, by means of expandable microspheres, is presented as an innovative approach addressing some of the previously mentioned issues. Furthermore, these expandable microspheres are embedded into a PDMS matrix, which composes a novel thermally responsive silicone elastomer composite actuator for liquid handling. Due to the merits of PDMS and expandable microspheres, the composite actuator's main characteristic to expand irreversibly upon generated heat makes it possible to locally alter its surface topography. The composite actuator concept, along with a novel adhesive PDMS bonding technique, is used to design and fabricate liquid handling components such as pumps and valves, which operate at work-ranges from nanoliters to microliters. The integration of several such microfluidic components promotes the development of disposable lab-on-a-chip platforms for precise sample volume control addressing, e.g. active dosing, transportation, merging and mixing of nanoliter liquid volumes. Moreover, microfluidic pumps based on the composite actuator have been incorporated with sharp and hollow microneedles to realize a microneedle-based transdermal patch which exhibits on-board liquid storage and active dispensing functionality. Such a system represents a first step toward painless, minimally invasive and transdermal administration of macromolecular drugs such as insulin or vaccines. The presented on-chip liquid handling concept does not require external actuators for pumping and valving, uses low-cost materials and wafer-level processes only, is highly integrable and potentially enables controlled and cost-effective transdermal microfluidic applications, as well as large-scale integrated fluidic networks for point-of care diagnostics, disposable biochips or lab-on-a-chip applications. This thesis discusses several design concepts for a large variety of microfluidic components, which are promoted by the use of the novel composite actuator. Results on the successful fabrication and evaluation of prototype devices are reported herein along with comprehensive process parameters on a novel full-wafer adhesive bonding technique for the fabrication of PDMS based microfluidic devices. / QC 20100817

With the completion of the human genome sequencing project, attention is currently shifting toward understanding how genetic variation, such as single nucleotide polymorphism (SNP), leads to disease. To identify, understand, and control biological mechanisms of living organisms, the enormous amounts of accumulated sequence information must be coupled to faster, cheaper, and more powerful technologies for DNA, RNA, and protein analysis. One approach is the miniaturization of analytical methods through the application of microfluidics, which involves the manipulation of fluids in micrometer-sized channels. Advances in microfluidic chip technology are expected to play a major role in the development of cost-effective and rapid DNA analysis methods. This thesis presents microfluidic approaches for different DNA genotyping assays. The overall goal is to combine the potential of the microfluidic lab-on-a-chip concept with biochemistry to develop and improve current methods for SNP genotyping. Three genotyping assays using miniaturized microfluidic approaches are addressed. The first two assays are based on primer extension by DNA polymerase. A microfluidic device consisting of a flow-through filter chamber for handling beads with nanoliter liquid volumes was used in these studies. The first assay involved an allelespecific extension strategy. The microfluidic approach took advantage of the different reaction kinetics of matched and mismatched configurations at the 3’-ends of a primer/template complex. The second assay consisted of adapting pyrosequencing technology, a bioluminometric DNA sequencing assay based on sequencing-bysynthesis, to a microfluidic flow-through platform. Base-by-base sequencing was performed in a microfluidic device to obtain accurate SNP scoring data on nanoliter volumes. This thesis also presents the applications of monolayer of beads immobilized by microcontact printing for chip-based DNA analysis. Single-base incorporation could be detected with pyrosequencing chemistry on these monolayers. The third assay developed is based on a hybridization technology termed Dynamic Allele-Specific Hybridization (DASH). In this approach, monolayered beads containing DNA duplexes were randomly immobilized on the surface of a microheater chip. DNA melting-curve analysis was performed by dynamically heating the chip while simultaneously monitoring the DNA denaturation profile to determine the genotype. Multiplexing based on single-bead analysis was achieved at heating rates more than 20 times faster than conventional DASH provides. / QC 20101008