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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
121

Educação à distância: Estudo de caso sobre a qualidade do processo pedagógico do curso de Ciência Política da UEA.

Lima, Sílvia Andréa Brandão de 13 October 2008 (has links)
Made available in DSpace on 2015-04-22T22:11:07Z (GMT). No. of bitstreams: 1 Dissertacao-Silvia Andrea Brandao de Lima.pdf: 453873 bytes, checksum: 5e5ba6153c2ac8b11ae69b85d862c2ee (MD5) Previous issue date: 2008-10-13 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The education, today, requires alternatives to win the challenges to extend the productivity of the systems of education and the institutions of Superior Education, to guarantee quality of the education, to form professors adequately and to keep brought up to date them. In this search a process is had in the long-distance Education that would be a modality that substitutes the actual education and yes an alternative of education for Brazil, bringing up to date concepts and knowledge, not assisting in the permanent one taken of conscience of the professionals on the advances promoted in its specific areas e, mainly, generating continued processes of access to the knowledge accumulated for the humanity the millions of citizens. In this quarrel the considered pointers are analyzed as form to inquire the quality in the pedagogical process of EAD in the course of Science Politics of the State University of Amazon, being verified themselves: the support to the pupil and the professor; the infrastructure; the development of the course and evaluation of the pedagogical process. The collection of data was carried through next to the titular pupils, professors and assistants and coordinators of the course. After the analysis of the data we got resulted that they had signaled for significant pointers of quality what of certain form they knock down the myth of that the quality is precarious in long-distance ensign. / A educação, hoje, requer alternativas para vencer os desafios de ampliar a produtividade dos sistemas de ensino e das instituições de Ensino Superior, garantir qualidade da educação, formar adequadamente professores e mantê-los atualizados. Nesta busca tem-se na Educação à Distância um processo que seria não uma modalidade que substitua a educação presencial e sim uma alternativa de educação para o Brasil, atualizando conceitos e conhecimentos, auxiliando na permanente tomada de consciência dos profissionais sobre os avanços promovidos em suas áreas específicas e, principalmente, gerando processos continuados de acesso ao conhecimento acumulado pela humanidade a milhões de cidadãos. Nesta discussão analisam-se os indicadores propostos como forma de averiguar a qualidade no processo pedagógico de EAD no curso de Ciência Política da Universidade Estadual do Amazonas, verificando-se: o apoio ao aluno e ao docente; a infra-estrutura; o desenvolvimento do curso e a avaliação do processo pedagógico. A coleta de dados foi realizada junto aos alunos, professores titulares e assistentes e coordenadores do curso. Após a análise dos dados os resultados sinalizaram para indicadores de qualidade significativos o que de certa forma derrubam o mito de que a qualidade é precária no Ensino à Distância.
122

O designer educacional e as competências profissionais: influências na seleção de recursos multimidiáticos

Pereira, Otacília da Paz 12 May 2011 (has links)
Made available in DSpace on 2016-04-29T14:22:52Z (GMT). No. of bitstreams: 1 Otacilia da Paz Pereira.pdf: 728029 bytes, checksum: f7264a6c8828db0b23d540713806f70b (MD5) Previous issue date: 2011-05-12 / The advent of new information and communication technologies has caused a very representative impact on the working market and on the form people face education, expanding learning spaces and possibilities; what makes many authors to consider the importance of educational design. This master's degree dissertation presents some insights about educational designer, a professional who works in the conception, implementation and management of educational projects that use the internet. The research focused on the attempt to identify the characteristics of the work developed by the educational designer, its profile and the main competencies that must guide such work. In order to develop this paper, we mentioned authors whose research is about educational design issues, in addition to those who are focused on the use of multimedia resources towards online education. In a qualitative field research, data collected from the discussion in a focal group with the educational designers who work at Senac São Paulo were used. From this group, the following categories emerged: multimedia resources and learning, educational designer‟s profile and educational designer‟s role. Result revealed there‟s still a lot of confusion between the role and the most qualified professional profile to work in this area, which seems to indicate the transitoriness of the professional and its main characteristics / O advento das novas tecnologias de informação e comunicação causou um impacto bastante representativo no mercado de trabalho e na forma como a educação é encarada, ampliando espaços e possibilidades de aprendizagem, o que fez com que muitos autores passassem a considerar a importância do design educacional. Esta dissertação de mestrado apresenta algumas reflexões sobre o designer educacional, profissional que atua na concepção, implementação e gerenciamento de projetos educacionais que utilizam a internet como meio. A pesquisa teve seu foco na tentativa de identificar as características do trabalho desenvolvido pelo designer educacional, seu perfil e as principais competências que devem nortear sua atuação. Para tanto, foram utilizados autores cuja pesquisa gira em torno das questões do design educacional, além daqueles cujo foco está mais voltado para a utilização de recursos multimidiáticos na educação on-line. Na pesquisa de campo, de caráter qualitativo, foram utilizados dados coletados a partir da discussão realizada em grupo focal com os designers educacionais que atuam no Senac São Paulo. Desse grupo, emergiram as seguintes categorias: recursos multimídia e aprendizagem, perfil do designer educacional e papel do designer educacional. Os resultados revelaram que há ainda muita confusão entre o papel e o perfil profissional mais indicado para atuação nesta área, o que parece indicar a transitoriedade da profissão e de suas principais características
123

Modelling atmospheric dispersal of fungal pathogens on continental scales to safeguard global wheat production

Meyer, Marcel January 2018 (has links)
The recent emergence of highly virulent strains of the pathogen causing wheat stem rust has been acknowledged as a threat to global food security. In infected wheat fields, vast amounts of pathogenic fungal spores are produced that can be carried away by wind. For targeted disease surveillance and control it is important to estimate when, where and how many fungal spores are dispersed from infected to susceptible wheat fields. In this study, high-performance computational resources are used to investigate long-distance dispersal revealing atmospheric pathways that connect entire continents. Mechanistic simulations of turbulent atmospheric spore dispersal are conducted. The analyses bring together a variety of data, including international field disease surveys and finely resolved meteorological model data. The UK Met Office's Langrangian stochastic particle dispersion model, NAME, is applied, extended and coupled to other models in a set of case studies. In the first case study, spore dispersal is analysed across Southern/East Africa, the Middle East, and Central/South Asia by simulating billions of stochastic trajectories of fungal spores over dynamically changing host and environmental landscapes. The circumstances under which virulent strains, such as Ug99, pose a risk to globally important wheat producing areas are identified. Simulation results indicate a negligible risk for dispersal from key wheat producing countries on the East African continent (Ethiopia, Kenya) directly to India and Pakistan. However, there is a considerable risk for atmospheric transport from the Arabian Peninsula to South Asia. Spore dispersal trends are quantified between all countries in the domain providing estimates which can be used to improve targeted sampling and control. In the second case study, dispersal from southern Africa to Australia is analysed. Simulation results, as well as data from phenotypic and genotypic analyses, support the hypothesis that extremely long-distance airborne dispersal across the Indian Ocean is possible, albeit rare. This indicates that the pathogen populations on the two continents are connected and underlines the importance of sharing surveillance intelligence between continents. The third case study focusses on Ethiopia, determining likely origins of strain TKTTF that recently caused severe epidemics in East Africa's largest wheat producing country. The analyses suggest inflow into Ethiopia from the Middle East via Yemen, consistent with field survey data. The risk for inflow of pathogens into Ethiopia from key neighbouring countries is ranked for different months of the wheat season. In the last results chapter a pilot study is summarized testing the feasibility of an automated short-term forecasting system for spore dispersal from the latest field disease detection sites. Whilst the functionality and practical relevance of the forecasting system is demonstrated, considerable challenges remain for testing the forecasts. The predictive simulation framework described in this thesis can be applied to any wheat producing area worldwide to assess dispersal risks. The research has broader relevance because long-distance dispersal is a key mechanism for the transmission of several crop and livestock diseases, and also plays an important role in other areas of ecology.
124

Long-distance procurement planning in global sourcing / L'optimisation de l'approvisionnement dans des zones géographiquement lointaines

Cao, Yu 05 February 2015 (has links)
Cette thèse porte sur l’optimisation de l’approvisionnement dans les zones géographiquement lointaines. Au moment de planifier des approvisionnements de matières premières ou de composants dans des pays lointains, la longue distance géographique entre l’acheteur et le fournisseur devient un enjeu essentiel à prendre en compte. Puisque le transport se fait souvent par la voie maritime, le délai d’approvisionnement est si long que les besoins peuvent évoluer pendant la longue période de livraison, ce qui peut engendrer un risque de rupture élevé. Cette thèse présente des approches adaptatives afin d’élaborer des plans d’approvisionnements lointains d’une manière rentable. Tout d’abord, nous proposons un cadre d’adaptation de la planification des approvisionnements lointains. Il déploie des techniques de prévision de la demande et des méthodes d’optimisation d’approvisionnements à horizon glissant. En utilisant ce cadre, nous transformons le problème de la planification sur l’horizon globale en plusieurs problèmes standards de lotissement avec demandes stochastiques sur des sous-horizons. Ce cadre permet aussi d’évaluer la performance sur une longue période des méthodes utilisées. Nous considérons ensuite la planification optimale d’approvisionnement sur les sous-horizons. Deux hypothèses de ruptures de stocks sont considérées: livraison tardive et vente perdue (ou sous-traitance). Nous développons des approches optimales ou quasi-optimales pour faire des plans d’approvisionnement tout en minimisant les coûts totaux prévus de commande, de stockage et de rupture sur les sous-horizons. Les méthodes proposées peuvent servir de repères pour évaluer d’autres méthodes. Pour chaque hypothèse, nous menons des expériences numériques pour évaluer les algorithmes développés et les approches adaptatives de planification globales. Les résultats expérimentaux montrent bien leur efficacité. / This research discusses procurement planning problems engaged in global sourcing. The main difficulty is caused by the geographically long distance between buyer and supplier, which results in long lead times when maritime transport is used. Customer demands of finished products usually evolve during the shipment, thus extra costs will be produced due to unpredictable overstocks or stockouts. This thesis presents adaptive planning approaches to make adequate long-distance procurement plans in a cost-efficient manner. Firstly, an adaptive procurement planning framework is presented. The framework deploys demand forecasting and optimal planning in a rolling horizon scheme. In each subhorizon, demands are assumed to follow some known distribution patterns, while the distribution parameters will be estimated based on up-to-date demand forecasts and forecast accuracy. Then a portable processing module is presented to transform the sub-horizon planning problem into an equivalent standard lot-sizing problem with stochastic demands.Secondly, optimal or near-optimal procurement planning methods are developed to minimize expected total costs including setup, inventory holding and stockout penalty in subhorizons. Two extreme stockout assumptions are considered: backorder and lost sale (or outsourcing). The proposed methods can serve as benchmarks to evaluate other methods. Numerical tests have validated the high efficiency and effectiveness of both sub-horizon planning methods and the overall adaptive planning approaches.
125

The regulation of psychological distance in long-distance relationships

Jimenez, Fanny V. 01 June 2011 (has links)
Diese Dissertation untersuchte individuelle Anpassungsprozesse, die psychologische Distanz in Fernbeziehungen (FB) reflektieren und regulieren. Es wurde postuliert, dass Beziehungsqualität und -stabilität ohne Präsenz des Partners gesichert wird, indem viele kompensatorischen Prozesse auf die wahrgenommene Verfügbarkeit des Partners (Bowlby, 1969/1980), abzielen. Die Ergebnisse zeigen dass die im Vergleich zu zusammenlebenden Paaren erhöhte Bindungsängstlichkeit bei FBs die inkonsistente Verfügbarkeit des Partners reflektiert, und insgesamt für die Beziehungsentwicklung weniger schädlich ist als Vermeidung. Bindung reguliert ferner die psychologische Distanz durch den Einfluss auf adaptive Kommunikationsprozesse. Wenn Aspekte wie sexuelle Zufriedenheit nicht kompensiert werden können, scheinen FBs ihre Einschätzung der Beziehung stark auf Momente des Sehens und die Beziehungsqualität zurückzuführen. / This dissertation proposed how relationship quality and stability in long-distance relationships (LDRs) is maintained without physical presence of the partner by basing compensatory processes on perceived partner availability, a core component of attachment theory (Bowlby, 1969/1980). The findings indicate that heightened anxiety reflects the inconsistent partner availability in LDRs, and is less detrimental to relationship development than avoidance. Attachment orientations further regulated the psychological distance between partners by influencing adaptive and protective communicative processes. There was indication that when relational aspects, such as sexual satisfaction, cannot be compensated for, LDRs base their evaluation on face-to-face experiences and general relationship quality indicators. 1
126

The Role Of Cultural Route Planning In Cultural Heritage Conservation The Case Of Central Lycia

Karatas, Esra 01 September 2011 (has links) (PDF)
The main subject of the thesis is planning &ldquo / cultural routes&rdquo / as a method for conservation of cultural and natural heritage areas at regional scale. Defining a framework of conceptual principles which should be considered in spatial planning of cultural routes and regional networks constitutes the major aim of the thesis. Within the scope of the study, a new developing concept recently, cultural routes are discussed as a tool for sustaining historic and local values of rural and archaeological landscapes. In this respect, the study is structured in two main parts. Firstly, conceptual background on the issue is discussed as the development of cultural route concept, definitions declared by international organizations working on the issue and principles of route planning. Secondly, based on the conceptual research, basic concepts and principles for route planning process is discussed through a case study. The case study for the thesis is selected as the Kas- Kekova region in Antalya, known as the Central Lycia in antiquity. Depending on the assessment of region&rsquo / s cultural landscape, the study is resulted by description of a spatial and conceptual framework for planning of a cultural route network in the region. Through areas rich in cultural and natural heritage, planning routes and networks at regional scale could be used as an effective tool for presenting and sustaining multivalent character of the place, and leading economic sectors which have effect on heritage.
127

A Study on Stroke Patients¡¦ Using Behaviors on ¡¨ Long-distance Community Health Care and Nursing Service¡¨Based on Health Behavior Model¡ÐConducting this research on an Unnamed Medical Center in the Southern Taiwan.

Kuo, Jui-Hsien 20 July 2008 (has links)
According to the statistics from Department of Health, Executive Yuan, in 2007,brain attack has been listed as the third of the top ten reasons for death. In Taiwan, the occurrence rate of brain attack on population above 35-year-old is 3/1000. And the total amount of population above 35-year-old is nearly 10,000,000. Then the latest number of occurrence of brain attack is approximately 30,000¡COnce the stroke happens, under such a circumstance, not only the expense of acute care increases but also the long-term medical care expense, the family and social cost will become larger. In view of that, the long-term care plan for stroke patient has been in urgent need. This research, ¡¨Long-distance Community Health Care Nursing Service for Stroke Patients¡¨, which is originated from a plan conducted by a medical center in Southern Taiwan. Based on Andersen¡¦s¡]1968¡^health behavior model as the theoretical structure¡Awe studied 102 patients who had a stroke (including high stroke risk patients)within one year in Kaohsiung. In this study, we applied JMP V6.0¡]SAS Institute, Cary, NC, USA¡^to analyzing case characters¡Aconducting univariate analysis by £q2 analysis and ANOVA. Then we applied multivariate logistic regression analyses to significant variables. We hope to find out the predisposition, enabling factors and need factor from those important predictor variables in ¡§Long-distance Community Health Care and Nursing Service¡¨. We research the differences of stroke patients¡¦ behaviors, providing those research results as the reference materials for related business promotional strategy in future, hoping to advance the quality of long-term care and nursing for stroke patients. In this study, total 102 copies of the questionnaire were sent out and returned, with a return rate of 100%, and 100 copies of questionnaire were effective. The research results show that: 1. Predisposition¡GThere are significant differences on these items--¡§Number of Children¡¨(Demography), ¡§Buddhist¡¨(Religion) and ¡§Those who have a health check within nearly one year¡¨(Health Concept). 2.Enabling factors¡GThere are significant differences on these items--¡¨Monthly Family Income¡¨(Economical factor),¡¨Monthly Balance of Payment¡¨(Economical factor) and ¡§Commercial Insurance¡¨(Insurance resource). 3.Need factors¡GPeople (those who think their health state stay at the average level), Health State (¡§High Blood Pressure and Heart Attack¡¨,¡¨Eye Diseases¡¨, ¡¨Cancer¡¨,¡¨Bone and Muscle¡¨,¡¨Asthma¡¨,¡¨Ears Disease¡¨and ¡¨Neural Diseases¡¨are involved. The behavior research includes ¡§Number of Accepting Medical Treatment per month¡¨(1 to 2 times), ¡§Activity in Daily Life ¡¨¡]ADL¡BIADL-Shopping Ability¡^and ¡§Functional Behaviors¡¨. 4. To sum up, the predisposition, enabling and need factors in this research could partially influence using behaviors¡Ain accordance with Andersen¡¦s inference in health behavior model. In conclusion¡G1.¡¨Long-term Community Health Care and Nursing¡¨ can be applied to stroke patients, which can help patients to build up effective self-management and advance life quality; 2. This service combines Medical Service Chain established by Information-Telegraphic Technology. Currently, blood pressure checking and telephone consultation are most popular service among interviewees, and other kinds of service are under development; 3. Stroke patients with different demographical characters show a great difference on using behaviors; 4. To speak briefly, this service can meet the ideal long-term care and nursing standard for local aging population.
128

World cities before globalisation : the European city network, A.D. 1300-1600

Verbruggen, Raf January 2011 (has links)
This dissertation is a quantitative study of the spatial business strategies of 130 late medieval and 16th-century European commercial and banking firms, the business networks of which have been put together for a structural analysis of the European city network between ca. 1300 and ca. 1600. Concretely this investigation has been carried out through the application of an interlocking network model – specifically developed for the study of the present-day global city network produced by the office networks of business service firms – to this historical case study, in order to challenge predominantly hierarchical conceptualisations of city networks which are often influenced by central place theory. After a methodological section, in which solutions are designed for reconciling the geographical model with the particularities of historical research, a first part of the analysis focuses on agency within the network, identifying and reconstructing the multiple spatial strategies used by the different agents. In a second part the overall structure and dynamics in the network are investigated, revealing the operation of Christaller's traffic principle, as well as a cyclical variation in emphasis on continental and maritime nodes within the European city network. More generally, this study demonstrates that the functioning of dynamic transnational networks based upon complementarity and cooperation rather than competition is not limited to our contemporary globalised world, but can also be found in particular historical societies.
129

Deguonies suvartojimo atliekant sunkėjantį krūvį pastovios fazės sąsaja su anaerobiniu pajėgumu / Oxygen consumption during increasing load constant phase interface with anaerobic capacity

Pukelevičiūtė, Asta 28 June 2011 (has links)
Pagrindiniai trys faktoriai lemiantys bėgikų ištvermę yra: maksimalus deguonies suvartojimas (MDS), laktato slenkstis (LS), bei bėgimo ekonomiškumas. Geriausi sportininkai turi panašų MDS, LS, bėgimo ekonomiškumą, bet įrodyta, kad raumenų faktoriai gali įtakoti ištvermę labiau, nei MDS. Yra duomenų, kad sportininkų raumenų galingumą įtakoja anaerobiniai adenozintrifosforo rūgšties (ATP) resintezės procesai, kurie gali turėti įtakos ir aerobinės ištvermės sportininkų varžybų rezultatams. Nustatyta, kad nuosekliai sunkėjančio krūvio (NSK) testo pabaigoje yra didesnis anaerobinis ATP resintezės indėlis. Vadinasi, didesnė raumenų masė ir jėga gali padėti toleruoti vis didėjantį ATP pareikalavimą ir parodyti didesnį deguonies suvartojimo pastovios fazės pasireiškimą. Labai įdomu būtų sužinoti, kaip anaerobinis pajėgumas gali įtakoti ilgų nuotolių bėgikų deguonies suvartojimo pastovios fazės trukmę. Mes manome, kad mūsų gauti tyrimo duomenys, leis geriau suprasti, kokį indėlį anaerobinis pajėgumas vaidina skirtingos deguonies suvartojimo pastovios fazės trukmės pasireiškime. Tyrimo tikslas – Nustatyti deguonies suvartojimą atliekant sunkėjantį krūvį ir pastovios fazės sąsaja su anaerobiniu pajėgumu. Tyrimo objektas: anaerobinio pajėgumo poveikis deguonies suvartojimo kaitai sunkėjančio krūvio metu. Tyrimo uždaviniai: 1. Palyginti aerobinio ir anaerobinio pajėgumo rodiklius tarp bėgikų pasiekiančių ilgesnę ir trumpesnę pastovią fazę. 2. Nustatyti koreliacinį ryšį tarp aerobinių ir... [toliau žr. visą tekstą] / Key words: aerobic capacity, anaerobic test, blood lactate concentration, long – distance runners. The three main factors determining runners endurance are: maximum oxygen consumption, lactate threshold and running economy. However, among the best sportsmen of similar maximum oxygen uptake, lactate threshold and running economy has been shown that muscular factors may influence the endurance more than the maximum oxygen consumption. There is evidence that the athletes' muscle power affects the ATP resynthesis anaerobic processes that may affect the aerobic and endurance sports events. showed that a consistent worsening at the end of the test is more anaerobic ATP contribution resynthesis, thus more muscle mass and strength can help to tolerate the increasing demand of ATP longer a burden, and to show increased oxygen consumption in stable phase of occurrence. It is interesting to learn how to influence the anaerobic capacity for long-distance runners' oxygen consumption in stable phase duaration. Therefore, our survey data to obtain a better understanding of the contribution of anaerobic capacity plays a different oxygen consumption constant phase term occurs. The study objective – identify the oxygen uptake during increasing workload constant phase interface with the anaerobic capacity. The object of the study – anaerobic capacity effects on the oxygen consumption change during increasing exercise load. Study tasks: 1. To compare the aerobicand anaerobic capacity in... [to full text]
130

Análise Metabolômica de Peperomia obtusifolia (L.) A. Dietr.

Souto, Augusto Lopes 26 February 2015 (has links)
Submitted by Viviane Lima da Cunha (viviane@biblioteca.ufpb.br) on 2016-03-29T14:36:11Z No. of bitstreams: 1 arquivototal.pdf: 5620328 bytes, checksum: cc12fb802b3be6b2f1e66e8519ace0cf (MD5) / Made available in DSpace on 2016-03-29T14:36:11Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 5620328 bytes, checksum: cc12fb802b3be6b2f1e66e8519ace0cf (MD5) Previous issue date: 2015-02-26 / The species Peperomia obtusifolia (L.) A. Dietr, which belongs to the Piperaceae family, is a well-known plant, distributed from Mexico to South America. Previous phytochemical studies demonstrated the presence of compounds from several classes, such as: lignans, flavonoids, amides, phenolic compounds and chromenes, which demonstrated interesting biological activities. This work describes the metabolomic analysis of Peperomia obtusifolia, which study in real time, the metabolism of this species in many kinds of scenarios. However, to accomplish this study, firstly it was developed a standard protocol, capable of preparing the samples in a reproducible way, enabling the following metabolomic studies to generate reliable results. Later, it was conducted the metabolomic study of Peperomia obtusifolia and its varieties, by fingerprinting analysis, in order to characterize the metabolomic profiles of each one of them. Furthermore, it was also evaluated the behavior of the species against the stress induced by the herbivory of Monoplatus sp. In general, the analyzes were conducted by NMR of 1H (which the data was interpreted by PCA), mass spectrometry, HPLC, GC and its hyphenated techniques. As a result, it was found that, despite the fact that the Peperomia obtusifolia varieties belong to the same species, they had different metabolomes. Regarding the study of the biotic stress against the plant, changes were detected on the metabolomic profile of the attacked plants, indicating a response against the herbivore attack. As a major result of this research, it can be highlighted the fact that the same changes were also detected on the metabolome of the intact plants closed to the attacked ones, indicating a sort of interplant communication by volatile compounds, capable to induce the neighborhood yet not attacked, to be prepared for an imminent attack, giving them a better chance of survival. / A espécie Peperomia obtusifolia (L.) A. Dietr, pertencente à família Piperaceae, é uma planta amplamente conhecida, cuja ocorrência vai desde o México até a América do Sul. Estudos fitoquímicos anteriores revelaram a presença de compostos de diversas classes, como: lignanas, flavonoides, amidas, compostos fenólicos e cromenos, os quais demonstraram atividade biológica bastante promissora. Este trabalho aborda a análise metabolômica de Peperomia obtusifolia, que visa estudar em tempo real o metabolismo desta espécie em diversos tipos de cenários. Entretanto, para que este estudo fosse realizado, primeiramente foi desenvolvido um protocolo padrão, capaz de preparar amostras de maneira reprodutível, possibilitando assim uma análise metabolômica que gerasse resultados fidedignos. Posteriormente foi realizado o estudo metabolômico de Peperomia obtusifolia e suas variedades, por análise de fingerprinting, que visa caracterizar os perfis metabólicos de cada organismo. Além disso, também foi avaliado o metaboloma da espécie frente ao estresse biótico provocado pela herbivoria do besouro Monoplatus sp. As análises das amostras foram realizadas por meio de RMN de 1H (cujos dados foram interpretados por PCA), espectrometria de massas, CLAE, CG e técnicas hifenadas. Como resultado, descobriu-se que apesar das variedades de Peperomia obtusifolia pertencerem à mesma espécie, elas possuíam metabolomas diferentes. Já em relação ao estudo de estresse biótico aplicado à planta, foram detectadas nas plantas predadas, alterações nos perfis metabolômicos, configurando-se uma resposta contra o ataque provocado. Considera-se um resultado de maior importância, o fato de que plantas intactas que estavam próximas às predadas, responderam ao ataque de maneira semelhante, indicando assim, uma comunicação inter-plantas por meio de compostos voláteis, capaz de induzir a vizinhança ainda não atacada a se preparar para reagir ao ataque, possibilitando assim, maior chance de sobrevivência.

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