The threatened Florida manatee (Trichechus manatus latirostris) migrates seasonally to warm-water refugia throughout the state of Florida due to metabolic requirements from low thermal conductance. Broward County’s two power plant refugia, Port Everglades (PEP) and Lauderdale (LPP), are known heavily-utilized aggregation sites for the Atlantic sub-population. Broward County collected relative abundance counts via aerial surveys from 2004–2013 siting 31,418 manatees during 169 surveys within 18 defined waterway zones. Counts during manatee wintering seasons were significantly different from January 2005-March 2008 and November 2008-March 2013, likely related to flight path and frequency standardization. Mean percentage of adults (90.12%) to calves (9.88%) demonstrates a higher usage by cow-calf pairs than other aggregation sites. Counts of manatees traveling south to Miami-Dade County comprised only 0.83% of all aerial counts, contrary to the theory of the extensive usage of Biscayne Bay foraging grounds. The LPP zone had 57.21% of all manatees with Port Everglades Inlet zone accounting for 23.88% and the South Fork New River zone with 5.95%. This study provided a baseline for pre-construction distributions prior to Port Everglades plant reenergization. With PEP construction now finished and LPP planned for reenergization in the next 10 years, monitoring data studies be compared to these baseline data to better assess the impact of the disruption of Broward County’s main refugia sources.

The seasonal distribution of the Florida manatee (Trichechus manatus latirostris) is influenced predominantly by feeding locations in the summer and proximity to warm-water refuges during colder months. Due to their limited ability to tolerate cold water, when water temperatures drop below 20°C manatees congregate around natural and artificial warm-water refuges, such as warm-water springs or power plant outfalls. Distribution may further be influenced by the tidal cycle through its impact on manatee movement and foraging. Although the importance of tide on manatee distribution and habitat selection has been acknowledged, it has yet to be studied quantitatively in respect to the manatee population in southeast Florida. The purpose of this study was to examine the effect of the tidal cycle on manatee occurrence at the Florida Power & Light (FPL) Port Everglades Power Plant. Walking surveys were conducted in Port Everglades during manatee season, November 15 – March 31, between 2004 and 2008. During the surveys, the number of manatees in four established locations was noted and the animals categorized based on size as either calf, juvenile, or adult. Water temperature data were also collected at four permanent sample locations. Because many surveys yielded zero manatees observed, data were analyzed using the zero-inflated negative binomial model. Although the manatees at the Port Everglades Power Plant must forage away from the warm-water refuge, my findings show no correlation between tidal state and total manatee occurrence at the FPL Port Everglades Power Plant. The results do, however, suggest that the probability of observing a cow/calf pair is greater during high tide when compared to low and mid-tides (P < 0.05). Total manatee occurrence and the presence of cow/calf pairs were both significantly correlated with water temperature (P < 0.05).

Florida manatees (Trichechus manatus latirostris) seek out warmer waters during winter months when ambient water temperatures drop below 20 degrees Celsius. Over time, manatees have discovered artificial warm water sites from power plant discharges in addition to natural sites such as springs and passive thermal refugia (PTRs). The Florida Power and Light (FPL) Port Everglades power plant in Broward County is one such artificial warm water refuge used by manatees. This plant was shutdown on July 16, 2013, and is expected to remain off line for at least three years during demolition and construction of a new facility. The purpose of this study was to determine changes in habitat usage and other responses to the disruption of a warm water refugia (the closure of a power plant) on Florida manatees within the greater Port Everglades region. From November 15, 2013 to March 31, 2014, manatees were counted at the site via shore line and aerial surveys. Environmental data were collected to determine variables correlated to manatee presence and absense. Two manatees were radio-tagged using remote sensing devices in an effort to identify alternative warm water refugia and feeding areas. Data were collected from state and local organizations and photo-identified manatees were used to analyze for trends in cold stress syndrome (CSS) lesions and watercraft scarring. Results confirmed that manatee presence was positively correlated with heater outlet temperature. Differences did not exist between the results of survey methods between the FPL Port Everglades (PE) and Fort Lauderdale (FL) power plant sites, however, differrences in the aerial survey counts showed an increased presence at the FL site during the colder month of January 2014. Data from tagging identified one manatee that preferred the FL site while another preferred PE, presumably due to more convenient access to feeding grounds in Biscayne Bay. Data from mortality events indicated a low number of CSS and watercraft deaths in Broward County as compared to other parts of the state of Florida. Further monitoring of the study area for the remainder of the shutdown period is recommended, as are improvements in survey design, expansion to include additional environmental data from FL, continued mortality statistic analysis and locating possible PTRs.

The largest Florida manatee (Trichechus manatus latirostris) aggregation at a natural warm-water refuge occurs in Kings Bay, Crystal River, FL. Over the last 32 years, the U.S. Fish and Wildlife Service and the State of Florida have created a network of manatee protection areas within Kings Bay including a year-round refuge designation and seven Federal manatee sanctuaries during the winter manatee season (November 15 – March 31). Aerial survey data collected between 1983 and 2012 was used to examine the seasonal change in manatee distribution within Kings Bay in order to assess the effectiveness of current sanctuary sizes and locations. Regression analysis indicated a significant change in manatee abundance among the winter seasons (p < 0.05). The average winter manatee counts increased by 4.81 animals per year over the 30 year period. In contrast, no significant changes in average or peak manatee abundance was detected among the summer seasons (p = 0.71 and p = 0.45 respectively). The average manatee counts increased by only 0.109 animals per year over the summer periods. Spatially explicit models using Geographic Information System (GIS) analysis revealed a strong correlation between high manatee density and artesian springs during the winter seasons. Highest abundances were identified at three locations: King’s Spring, Three Sisters Springs, and Magnolia Springs. These three locations coincide well with pre-existing sanctuary designations, but additional coverage is needed to support the overflow of manatees outside of sanctuary boundaries. Manatees continued to use Kings Bay in the summer seasons but in lower numbers and densities. Because density patterns were not uniform across summer periods, a heavier reliance on boat speed regulation is recommended to provide adequate protection to the endangered Florida manatee. Within a habitat type, the Magnolia Springs, South Banana Island, and Three Sisters Springs sanctuaries exhibited a significant influence on manatee density, suggesting differences in quality among sanctuaries. Years coinciding with extreme cold weather events also had a significant influence on manatee density. Using GIS to investigate seasonal shifts in manatees can be very informative regarding many issues including habitat selection and may improve the design and management of protected areas.

State and Federal agencies have created sanctuaries and speed zones to help reduce manatee mortality while incorporating the recreational and commercial resource needs of these same habitats for humans. Specific habitat resources are considered necessary to increase manatee survivorship. We have only recently begun to address how manatees use some of these resources based on physiological or reproductive strategies. In this study, I quantified patterns of habitat use during seasonal and diel periods for different sex and reproductive manatee classes using data from a radio-telemetry study conducted by the Florida Fish and Wildlife Conservation Commission during 1991-1996. I used five environmental geographic data layers: bathymetry, distance to seagrass, distance to shoreline, distance to warm water refuge sites, and distance to fresh water sources, to discriminate seasonal and diel habitat use patterns for different manatee classes: males (M), females with calves (FWC), and females without calves (FNC). Mean occupancy values were calculated for environmental variable locations and seasonal, diel, and manatee class differences were tested using a Multi-Response Permutation Procedure (MRPP). Nonmetric Multidimensional Scaling (NMS) was used to visualize the ordination patterns of the manatee classes and to assess importance of correlated environmental variables. Significant differences in habitat use were noted between summer and winter based on distances to warm water, seagrass, and fresh water sources but similar habitat use patterns were exhibited within summer diel periods among manatee classes. All manatee classes appeared to have used a higher proportion of locations closer in proximity to seagrass at night than day in winter indicating a disproportionate difference in feeding bouts between diel periods. These differences may be attributed to adjusting feeding strategies to reduce thermoregulatory costs or to decrease human interactions. Differences in patterns were exhibited for the winter diel periods specifically for the FWC manatee classes during winter days. FWC had a higher proportion of locations within the warm water refuges during the day indicating a possible trade off situation between food consumption and thermal exposure. This study demonstrates coarse and fine scale patterns of variation in habitat use for manatees both seasonally and daily within winter. It also suggests that during winter months, manatees were not just utilizing their habitat but they appeared to have preferences and selection for certain habitat types. Recovery of a species is greatly enhanced when patterns of habitat use within the species' environment has been clearly defined. Understanding more specifically what types of habitats manatees choose might allow management to adjust strategies for protection of key habitats while encouraging further recovery of this species.

O peixe-boi marinho, Trichechus manatus manatus, à um mamÃfero com dieta herbÃvora, e distribuiÃÃo costeira em rios, estuÃrios e Ãguas litorÃneas da Zona Tropical brasileira. No Estado do Cearà ocorre em duas Ãreas distintas, no extremo dos seus setores leste e oeste. No litoral leste nÃo existe nenhuma Unidade de ConservaÃÃo com abrangÃncia marinha que restrinja os impactos causados ao meio ambiente e à populaÃÃo do peixe-boi, tais como urbanizaÃÃo, turismo e ocupaÃÃo de praias, dunas, falÃsias e manguezais. Sendo considerado pela UniÃo Internacional para ConservaÃÃo da Natureza como âespÃcie vulnerÃvelâ e estando inserido na Lista Oficial de EspÃcies da Fauna Brasileira AmeaÃadas de ExtinÃÃo de acordo com o IBAMA, corre risco de extinÃÃo extremamente alto num futuro prÃximo. Os objetivos desta DissertaÃÃo foram determinar a distribuiÃÃo espacial e padrÃes de uso da Ãrea, escolha da metodologia mais adequada ao monitoramento da populaÃÃo, e realizar levantamento das Ãreas de alimentaÃÃo e fontes e Ãgua doce provavelmente utilizadas pelos animais. A base de dados foi obtida atravÃs de prospecÃÃes aÃrea, realizada de junho/2003 a outubro/2004, totalizando 11 sobrevÃs e 24 horas de esforÃo de monitoramento, e nÃutica, realizada de agosto/2003 a novembro/2004, num total de 10 embarques e 157 horas de esforÃo de monitoramento. Dentre os mÃtodos de prospecÃÃo aÃrea e nÃutica, o primeiro gera erros devido à turbidez da Ãgua, e dificuldades de identificaÃÃo e contagem dos indivÃduos, enquanto o segundo, por sua maior proximidade em relaÃÃo aos animais, mostrou-se mais eficiente. O status da populaÃÃo do peixe-boi marinho foi avaliado atravÃs dos seguintes parÃmetros: (a) conservaÃÃo do habitat, com Ãnfase para a manutenÃÃo do territÃrio; (b) acesso a fontes de Ãgua doce e a Ãreas preferenciais de alimentaÃÃo e reproduÃÃo; (c) geraÃÃo de condiÃÃes ambientais Ãtimas para os cuidados parentais. Os fatores de ameaÃa de extinÃÃo nessa Ãrea estariam relacionados com reduÃÃo das fontes de alimento e Ãgua doce, assoreamento do Rio Jaguaribe, alÃm de captura acidental por aparelhos de pesca. Estes se traduzem nas seguintes conseqÃÃncias: reduÃÃo do habitat; aumento da frequÃncia de encalhes de recÃm-nascidos; aumento da mortalidade de adultos. A regiÃo de Picos, com destaque para o Banco dos Cajuais, foi definida como Zona de Alta Densidade, com padrÃo de distribuiÃÃo âaltamente agregadaâ por causa da abundÃncia de macroalgas e do menor fluxo de barcos motorizados. Dentre as recomendaÃÃes para se minimizar os riscos de extinÃÃo do peixe-boi, destacam-se: (a) desenvolvimento de campanhas educacionais que ressaltem aspectos importantes da conservaÃÃo do ambiente marinho; (b) reintroduÃÃo de espÃcimes em locais onde o declÃnio populacional à evidente; (c) criaÃÃo de unidades de conservaÃÃo com abrangÃncia marinha e aplicaÃÃo e fiscalizaÃÃo da legislaÃÃo pertinente ao habitat do peixe-boi e (d) monitoramento sistemÃtico da populaÃÃo no litoral leste para determinaÃÃo dos padrÃes ambientais que influenciam na ocorrÃncia do peixe-boi no municÃpio de IcapuÃ, litoral leste do CearÃ. / The Antillean manatee, Trichechus manatus manatus, is a mammal with an herbivorous diet and costal distribution in rivers, estuaries and inshore waters of the Brazilian Tropical Zone. In Cearà State, the species may found in the outermost ends of its western and eastern sectors. In the eastern sector there are no Marine Conservation Units to ward off impacts to the environment and the manatee population, such as urban development, tourism and occupation of beaches and mangrove forests. Manatees have been classified by the International Union for Nature Conservation as a âvulnerable speciesâ and included on the Official List of Endangered Species of the Brazilian Fauna. According to the Brazilian Institute for the Environment, it runs an extremely high risk of extinction in the near future. The objectives of this Thesis have been to determine the spatial distribution and patterns of usage of the living area, choice of the most appropriate methodology for population monitoring and mapping of feeding grounds and freshwater sources likely to used by the animals. The database was obtained by means of aerial surveys from June, 2003 to October, 2004, making up a 24-hour monitoring effort during 11 overhead flights, as well as nautical surveys performed from August, 2003 to November, 2004, making up a 157-hour monitoring effort during 10 voyages. Of the two prospecting techniques, the aerial one was less practical because of water turbidity, which made it difficult to identify and count manatee specimens, whereas the nautical one, because of its nearness to the targets, proved to the most efficient. The status of the Antillean manatee population was evaluated through the following parameters: (a) habitat conservation, with emphasis on territory maintenance; (b) access to freshwater sources and areas suitable for feeding and reproduction; (c) yielding of optimum environmental conditions for parental care. The threatened extinction in the studied area is related to factors such as decreasing of food and freshwater sources, silting-up of the Jaguaribe River, by-catch by trawl and gillnet fishing. The worrying outcome of such a scenario is likely to result in a reduction of the habitat size; increase in the straying of newborns plus an increase in adult mortality rates. The Picos region, specially the Cajuais sandbank, was defined as a High Density Zone, which displays a highly aggregate distribution because of seaweed abundance and smaller deployment of fishing boats. Among the suggestions for lowering the extinction risks of the Antillean manatee in Eastern Cearà State, the following ones stand out; (a) development of education campaigns which deal with the main aspects of environment preservation; (b) reintroduction of specimens in zones where population decline is evident; (c) installation of Marine Conservation Units and enforcement of the pertinent management measures and (d) constant monitoring of the population in the known habitat to determine environmental patterns which influence manatees in Icapuà county, eastern Cearà State.

The metabolic hormones ghrelin, growth hormone, and insulin-like growth factor I are influenced by developmental age, sex, and nutritional status in domestic and free-ranging species. However the role these hormones play has not previously been explored in sub-tropical/ tropical mammals. Furthermore, the seasonality of species with less dynamic environmental cues, such as the West Indian manatee, have not been studied. The West Indian manatee is and endangered species distributed in the southeastern United States and throughout the Caribbean basin, and its nutritional physiology is influenced by environmental factors. Understanding the hormone response to nutritional status in this species and its seasonality will enhance our knowledge of the links between season, nutrition, and life history. The purpose of this research is to understand the biology and seasonal patterns of metabolic hormones in free-ranging manatees which will allow us to assess the nutritional status of wild populations. The research objectives include validation assays to accurately quantify hormone concentrations in manatees. Hormones were quantified in manatee serum using heterologous radioimmunoassay. Hormones were then evaluated between summer, fall, and winter and compared to body composition. Developmental patterns were also assessed. Lastly, hormones were examined between Florida and Antillean manatee populations. Manatees exhibited differences in GH, IGF-I, and body composition demonstrating seasonality similar to other species. Manatees exhibited differences between age classes suggesting decreased growth rate as the animals age. Differences were detected between populations. This research suggests that ghrelin, GH, and IGF-I can be used to indicate nutritional status and detect seasonality and developmental age in the manatee. This could prove to be a valuable tool in rehabilitation facilities and during captures and health assessments to provide better veterinary care and further improve overall health and body condition to better manage the survival of the species.