Esta dissertação trata de duas obras autobiográficas escritas por autoras nativas que ganharam reconhecimento na década de 70: Bobbi Lee Indian Rebel (1975), da nativo-canadense Lee Maracle, e The Turquoise Ledge: a Memoir (2010), da nativo-americana Leslie Marmon Silko. A importância destas autoras para a Renascença Nativo-Americana/Canadense é inegável, e cada uma delas contribuiu fazendo uso de estratégias diferentes: enquanto Maracle começou sua carreira com Bobbi Lee Indian Rebel, de cunho autobiográfico, Silko esperou mais de trinta anos para publicar The Turquoise Ledge. A problematização de se ver estas obras pelo olhar estritamente ocidental, ou estritamente nativo, é discutida, assim como o aparentemente inevitável tom político dessas narrativas. Ainda que mais de três décadas separem a publicação das obras selecionadas, perguntas como: Estas obras podem ser consideradas literatura?, Elas têm como principal propósito engrandecer feitos pessoais das autoras?, ou Como essas narrativas contribuem para o empoderamento do povo Nativo? podem nunca chegar a serem respondidas, mas, de fato, incitaram a escrita desta dissertação e nortearam nossa análise / This dissertation brings to the fore two autobiographical works by Native women authors who first gained recognition in the 1970s: Bobbi Lee Indian Rebel (1975), by Native-Canadian Lee Maracle and The Turquoise Ledge: a Memoir (2010), by Native-American Leslie Marmon Silko. These womens undeniable importance to the Native American/Canadian Renaissance is clear, and each of these authors decided to contribute to Native literature using different strategies: while Maracle started her career with Bobbi Lee Indian Rebel, an autobiographical work, Silko waited over thirty years to publish her The Turquoise Ledge. The problematization of seeing either works strictly through Western or strictly through Native perspectives is also addressed here, along with the apparently inevitable political tone present in both narratives. Despite the fact that the two selected works have been written over three decades apart, questions such as: Can these works be considered literature?, Do they have as main purpose to highlight the authors personal accomplishments? or How do they work to empower the Native people? may never be answered, but they did incite the writing of this dissertation and guided our analysis

Submitted by (edna.saturno@ufrpe.br) on 2017-05-17T15:31:54Z No. of bitstreams: 1 Marta Gerusa Soares da Silva.pdf: 1019521 bytes, checksum: dafe6a7ce15446dca593cbcc3e9179b2 (MD5) / Made available in DSpace on 2017-05-17T15:31:54Z (GMT). No. of bitstreams: 1 Marta Gerusa Soares da Silva.pdf: 1019521 bytes, checksum: dafe6a7ce15446dca593cbcc3e9179b2 (MD5) Previous issue date: 2010-02-26 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The aim of this study was to evaluate the dynamics of the association of „capim-de-raiz‟ (Chloris orthonoton Doell.) and „capim-milhã‟ [Brachiaria plantaginea (Link.) Hitchc.], concerning growth, persistence and production, as well to morphanatomically characterize the legume „orelha de onça‟ (Macroptilium martii Benth.). In order to evaluate the dynamics of the association „capim-de-raiz‟ and „capim-milhã‟, two experiments were carried out: I. Evaluation of deferred native pasture concerning botanical composition and forage mass; II. Growth dynamics of „capim-milhã‟ and „capim-de-raiz‟. Experiment I was conducted at the Experimental Station of IPA, in São Bento do Una - PE from 2007 to 2008 with different evaluation periods for forage mass and botanical composition. Experiment II was conducted at a greenhouse in the Animal Science Department - UFRPE, from 09.26.06 to 07.26.07. Soil blocks with the plant community were taken from native pasture area of the Experimental Station of Instituto Agronômico de Pernambuco - IPA, in São Bento do Una - PE, and placed in plastic pots remaining at a greenhouse in UFRPE. Pots were submitted to four irrigation schedules: Irrigation I - irrigation at the beginning of the experiment; Irrigation II - started two months after Irrigation I; Irrigation III - started four months after irrigation I and Irrigation IV - started six months after irrigation I. After the initial irrigation of each period, all pots were irrigated every seven days until drainage and regrowth number, seedling number, plant number and inflorescence number were evaluated. Morphanatomical characterization of „orelha de onça‟ leaf was analyzed with optical microscopy in cross-sections confectioned laminae. 10 families, 15 genera and 18 species were identified in the deferred pasture. In botanical composition from May to August 2007, „capim-milhã‟ was the main component from 60 to 90 days with 46.75 and 52.09%, respectively. In August 2007, other species participated with 57.75% of the botanical composition; at the end of evaluation in year 2008 other species occupied 100% of the composition. The highest forage mass (kg DM/ha) for „capim-milhã‟ (2.736) occurred at 90 days, for other species (3.141) and „capim-de-raiz‟ (2.701) at 120 days. In soil blocks it was observed that regrowth occurred only after 30 days of assessment for initial irrigation and the highest number of seedlings to the pots occurred at 30 days. It was observed at 60 days the largest number of grasses for irrigation III and at 90 days for irrigation IV, and that most of inflorescence appeared at 90 days. In deferred area the use of stockpiled forage should preferably take place from 60 to 90 days. The main form of establishment of „capim-de-raiz‟ was by germination and at 150 days it was observed population growth of other species. It was observed paracytic and amphistomatic stomata, simple and uniseriate hairs, palisade and spongy chlorophyll parenchyma in „orelha de onça‟ leaf blade. In stem vascular system it was observed a range of libero-ligneous beams in the internodes area, separating cortex of medulla. Foliar and stem anatomical characteristics are common to legumes. / O objetivo deste trabalho foi avaliar a dinâmica da associação capim-de-raiz (Chloris orthonoton Doell.) e capim-milhã [Brachiaria plantaginea (Link.) Hitchc.], quanto ao crescimento, persistência e produção, bem como caracterizar morfoanatomicamente a leguminosa orelha de onça (Macroptilium martii Benth.). Para avaliar a dinâmica da associação capim-de-raiz e capim-milhã, foram realizados dois experimentos: I. Avaliação de um pasto nativo diferido quanto à composição botânica e massa de forragem; II. Dinâmica do crescimento do capim-milhã e capim-de-raiz. O experimento I foi conduzido na Estação Experimental do IPA, no município de São Bento do Una - PE, de 2007 a 2008, sendo utilizados diferentes períodos para avaliação da massa de forragem e composição botânica. O experimento II foi realizado em telado no Departamento de Zootecnia - UFRPE, durante o período de 26.09.06 a 26.07.07. Cubos de solos com a comunidade vegetal foram retirados de área de pastagem nativa, da Estação Experimental do Instituto Agronômico de Pernambuco - IPA, no município de São Bento do Una - PE, e colocados em vasos plásticos permanecendo em telado da UFRPE. Os vasos foram submetidos a quatro épocas de início de irrigação: Irrigação I ¿ irrigação por ocasião da implantação do experimento; Irrigação II ¿ iniciada dois meses após a irrigação I; Irrigação III ¿ iniciada quatro meses após a irrigação I e Irrigação IV ¿ iniciada seis meses após a irrigação I. Após a irrigação inicial de cada período, todos os vasos foram irrigados a cada sete dias até drenagem e foram avaliados o número de rebrotes, número de plântulas, número de plantas e número de inflorescências. A caracterização morfoanatômica foliar da orelha de onça foi analisada em microscopia óptica em lâminas confeccionadas em secções transversais. Foram identificadas 10 famílias, 15 gêneros e 18 espécies no pasto diferido. Na composição botânica de maio a agosto de 2007, o capim-milhã foi o componente principal dos 60 aos 90 dias com 46,75 e 52,09%, respectivamente. Em agosto de 2007, outras espécies participaram com 57,75% da composição botânica; no ano de 2008 ao final da avaliação, outras espécies ocuparam 100% da composição. As maiores massas de forragem (kg de MS/ha) para o capim-milhã (2.736) foram aos 90 dias, e para outras espécies (3.141) e capim-de-raiz (2.701) aos 120 dias. Nos cubos de solo, observou-se que o rebrote ocorreu apenas aos 30 dias de avaliação para irrigação inicial e o maior número de plântulas para os vasos ocorreu aos 30 dias. Foi observado aos 60 dias o maior número de gramíneas para irrigação III e aos 90 dias para irrigação IV, e que a maioria das inflorescências surgiu aos 90 dias. Em área diferida, o aproveitamento da forragem acumulada deve ocorrer preferencialmente dos 60 aos 90 dias. A principal forma de estabelecimento foi por germinação de sementes e aos 150 dias observou-se crescimento populacional de outras espécies. Foram observados na lâmina foliar da orelha de onça estômatos paracíticos e anfiestomáticos, pelos simples e unisseriados, parênquima clorofiliano em paliçada e esponjoso. Nos sistemas vasculares do caule observou-se, nas áreas dos entrenós, uma faixa de feixes líbero-lenhosos separando o córtex da medula. As características anatômicas foliares e caulinares são comuns às leguminosas.

Syftet med denna studie är att undersöka fördröjningsskillnader inom användargränssnitt mellan native­utvecklade appar (utveckling till varje plattform) och appar av typen generated apps. Eftersom arbetet syftar till att bidra med information om prestanda ansågs en experimentell metod vara det bästa valet. Mätning av laddningstider gjordes med hjälp av en videokamera som filmade utförandet av experimenten vilket gjorde metoden simpel och liknar det som en användare kommer att uppleva. Avgränsning till plattformarna Android och iOS gjordes där Xamarin valdes som ramverk inom tekniker som skapar generated apps. Mätdata från experiment som undersökte laddningstider, experiment med användare som hanterade listors respons samt undersökning av CPU­ och minnesanvändning tyder på ett återkommande mönster. Xamarin Forms med XAML är den teknik som presterat sämst under experimenten som sedan följs av Xamarin Forms. Xamarin Android/iOS hade inte lika stora prestandaförluster jämfört med native­utvecklade delar. Generellt hanterar Xamarin Forms telefonens resurser sämre än vad Xamarin Android/iOS och native gör. Resultat från studien kan användas som beslutsstöd vid val av teknik. Studien bidrar även med data som kan användas vid vidare forskning inom området. / The purpose of this study is to examine differences in delay during calculation and presentation of interfaces executing on different techniques. Specifically between native developed apps and generated apps. Since the work aims to contribute with information regarding performance, an experimental method was considered to be the best choice. Measurements of loading times was made with a video camera that captured the execution of the experiments which made the method simple and captures what the users would have experienced. Demarcation to the platforms Android and iOS was made and Xamarin was chosen as the framework for creating generated apps. Data from experiment that examined loading times, experiment with users that dealt with the responsiveness of lists and the analysis of CPU and memory usage indicate a recurring pattern. Xamarin Forms with XAML is the technique that has performed worst during the experiments, followed by Xamarin Forms. Xamarin Android/iOS didn’t have as big performance loss. Xamarin Forms does not manage the phones resources as efficient as Xamarin Android/iOS and native developed apps does. The results from this study can be used as material for making decisions witin organisations when choosing technology for creating apps. The study also contribute with data that can be used by other researchers.

This study examines the extent of the impact of proficiency and language use on sociophonetic variation in Indian English (IE). It is based on an oral corpus using the methods and tools of the PAC project and derived from a pool of South Delhi-based highly proficient speakers. The investigation was conducted using quantitative and qualitative methods and focused on two understudied variables: (1) the fricative realisation of th, and (2) the realisations of the vowels in words of the NORTH and FORCE lexical sets. First, the results demonstrate that a significant amount of variation which cannot be accounted for by the traditional age, gender and social class factors can be explained by the language use parameter. A degree of correlation was found between the volume of use of English in a range of domains, and how speakers take advantage of the sociolinguistic potential of prestigious forms. This offers indications on the location of the leaders of the linguistic change. The second central feature of this study is derived from the investigation of the NORTH versus FORCE distinction. It is argued that the general maintenance of this distinction in IE provides evidence for the endo-normative nature of this variety. In the light of these findings, issues ultimately relating to the debate over nativeness are discussed.

Some researchers that work with metaphor theory claim that metaphors and figurative language are understood and processed just as easily as literal language. However, as this thesis will explore in detail, other research indicates that such is not always the case. That is, if the category of metaphor is further subcategorized into conventional and non-conventional metaphor, the scope will change because of the fact that it is possible to argue that non-conventionalized metaphors require a more conscious path of processing. In order to explain this alternative path, there are two primary approaches to language processing worth introducing: implicit and explicit. These approaches vary in required attention and speed of processing. With regards to conscious effort, these approaches are rather similar to the way in which we process conventionalized and non-conventionalized metaphors. Conventional metaphors are processed more quickly and easily than non-conventional ones. Hence, the claim that all metaphors are similarly processed may not always be true. Furthermore, an individual’s level of proficiency presumably correlates with speed in language processing. However, if non-conventional metaphor requires a more deliberate path of processing, this thesis assumes that the processing of this type of metaphor will be relatively unaffected by proficiency level, thus causing informants to process them in similar manners. In this thesis, 24 non-native speakers (NNS), categorized into intermediate proficient and advanced proficient, and seven native speakers (NS) were tested with an RT-test on subjective metaphor comprehension. Results were compared using mean response times and standard deviations, as well as looking at correlations and coefficient of variation. The results showed a distinct difference in processing speed with conventional metaphors being processed significantly faster. Moreover, the findings indicate that conventional metaphor processing speed seems to be predicted by proficiency, whilst non-conventional processing speed is not. The RT differences remained relatively consistent in both conventional and non-conventional metaphor processing, but when taking correlations, variance and coefficient of variation into consideration, the findings indicate that these other factors help level out the differences in non-conventional metaphor processing in more subtle ways than simply by RT’s.

Ce travail de thèse porte sur le développement de méthodes en spectrométrie de masse (MS) et mobilité ionique (IM-MS) supramoléculaires pour la caractérisation fine de complexes protéine-ligand et d’assemblages protéiques hétérogènes de hauts poids moléculaires. L’optimisation instrumentale apportée à l’étude de ces systèmes, permet d’étendre le potentiel de ces deux approches en biologie structurale. Le criblage de complexes protéine-ligand permet ici une détermination de leurs propriétés d’interaction et la mise en évidence de subtils changements de conformation induits, pouvant être suivis au cours du temps. L’application de ce couplage à l’analyse de complexes multi-protéiques, réfractaires aux techniques conventionnelles, donne accès à la topologie de ces assemblages, facilitant la proposition de modèles structuraux. Enfin, l’apport récent de la haute résolution en MS native est ici illustré à travers l’étude de protéines complexes et hétérogènes : les anticorps thérapeutiques et leurs conjugués. Ces développements permettent de repousser certaines limites en MS native et IM-MS native, élargissant leurs perspectives d’application dans la recherche et l’industrie pharmaceutique. / This PhD thesis aims at developing methods in native mass spectrometry (MS) combined with ion mobility (IM-MS) to characterize protein-ligand complexes and large protein assemblies. Fine-tuning of instrumental settings allowed expanding the scope of these approaches in structural biology. Real-time monitoring of protein-ligand complexes by native MS and IM-MS enabled to screen their binding properties while depicting subtle conformational changes induced upon binding. Applying these methods to refractory multi-protein complexes provided insights about their topology, making structural modeling easier. Finally, benefits from high-resolution native MS were highlighted through the characterization of heterogeneous systems, including monoclonal antibodies and their drug conjugates. Here, these developments enable to push some technical limits one step forward, increasing the potential of native MS and IM-MS both in academic research and pharmaceutical industry.

Ce travail de thèse porte sur développement de méthodes en spectrométrie de masse structurale pour l’analyse de protéines recombinantes et de leurs complexes associés. L’objectif central s’est porté sur des développements méthodologiques en échange hydrogène/deutérium couplé à la spectrométrie de masse (HDX-MS). Les techniques biophysiques de caractérisation structurale à haute résolution comme la cristallographie ou la RMN se heurtent régulièrement à des problèmes de productions de cristaux, de taille de complexes analysables ou encore de quantité de matériel nécessaire importante. Le développement de méthodes spécifiques HDX-MS a permis de réaliser une caractérisation structurale de systèmes protéiques variés, et réfractaires aux approches haute résolution. La combinaison de cette approche à différents outils de MS structurale est aussi illustrée, et montre tout son intérêt pour l’obtention d’informations à résolution augmentée. / This thesis work focuses on development of structural mass spectrometry methods for the analysis of recombinant proteins and their associated complex. The central objective has focused on the development of hydrogen/deuterium exchange coupled to mass spectrometry approaches (HDX-MS). The high resolution biophysical techniques for structural characterization such as crystallography or NMR regularly face problems of crystal productions, size analyzable complex or quantity of material required. The development of specific HDX-MS methods allowed the characterization of various, and refractory protein systems to high resolution approaches. The combination of this approach with complementary structural MS tools is also illustrated, and shows its interest to obtain increased resolution information.

Fundação de Amparo a Pesquisa no Estado do Rio Grande do Sul / Paspalum notatum, native grass biome Pampa, Rio Grande do Sul, has high forage value, but has difficulty germinating seeds with low viability. Inserted in this context are the promoters of germination and biomass, biostimulants stimulate® and bioagent Trichoderma spp. by mechanisms that influence the metabolism of action of the species. The present study investigated the promotion of seed germination and development of P. notatum. Through the Allium cepa test in ten treatments (four replicates per treatment), eight with metabolites of Trichoderma spp. and two controls in the absence of metabolites were selected isolates of trichoderma biological agent. The test of sanity, the identification of fungi associated with seeds used the methods of filter paper and plating on solid agar PDA (potato dextrose agar). With the in vitro technique of direct confrontation of the antagonist action on three fungal contaminants of highest incidence was observed. In a preliminary test, pre-treatment is selected to germination of P. notatum. Seed germination in greenhouse consisted of 16 treatments with four replicates, the control treatments, the three isolates, the stimulate®, the combination of each of the three isolates stimulate®, all with and without pretreatment, analysis of the data was through the Genes program. For reviews of morphogenesis, four were randomly chosen tillers per treatment, they monitored for the appearance and elongation and leaf senescence. This monitoring was performed two times per week. The top three trichoderma isolates selected in test A. cepa strain were 2B2, C1, 2B12. The removal of the seed coat structures of P. notatum was the pre-treatment showed the best performance with 34.5% twinned seeds. In the test of sanity the fungal genera Aspergillus, Curvularia, and Fusarium Geniculosporium were identified, isolated and 2B2 of Trichoderma spp. efficient for direct to these fungal genera confrontation. For the IVE (index emergency speed), the combination treatments of biological powder 2B12 isolated from trichoderma and biostimulating Stimulate® and only Stimulate® biostimulating with pretreatment and without pretreatment combination of biological powder isolated 2B2 Trichoderma with biostimulating Stimulate®, achieved the best results, with 14.48, 13.93 and 12.75, respectively. The biostimulating stimulate® promotes the rate of speed of emergence of seeds of P. notatum and the production of dry matter, the initial rate of elongation of the sheet (TAIF) positively interfere in the dry weight of shoots. / Paspalum notatum, gramínea nativa do bioma Pampa, Rio Grande do Sul, possui alto valor forrageiro, porém apresenta dificuldade de germinação de sementes, com baixa viabilidade. Inseridos neste contexto estão os promotores de germinação e biomassa, bioestimulante Stimulate® e o bioagente Trichoderma spp. que através de mecanismos de ação influenciam no metabolismo da espécie. O presente trabalho objetivou estudar a promoção da germinação das sementes e desenvolvimento de P. notatum. Através do teste de Allium cepa em dez tratamentos (quatro repetições por tratamento), oito com metabólitos de isolados de Trichoderma spp. e dois controles na ausência de metabólitos foram selecionados isolados do agente biológico trichoderma. O teste da sanidade, a identificação dos fungos associados às sementes utilizaram-se os métodos do papel de filtro e do plaqueamento em meio ágar sólido BDA (batata dextrose ágar). Com a técnica in vitro de confrontação direta foi observada a ação do antagonista sobre três fungos contaminantes de maior incidência. Em teste preliminar, selecionou-se o pré-tratamento para a germinação das sementes de P. notatum. A germinação das sementes em casa de vegetação constou de 16 tratamentos, com quatro repetições, os tratamentos controle, os três isolados, o stimulate®, a combinação de cada um dos três isolados mais stimulate®, todos com e sem o pré-tratamento, análise dos dados foi através do programa Genes. Para as avaliações das características morfogênicas, foram escolhidos aleatoriamente quatro perfilhos por tratamento, sendo os mesmos monitorados quanto ao aparecimento e alongamento de folhas e senescência. Este monitoramento era realizado duas vez por semana. Os três melhores isolados de trichoderma selecionado no teste de A. cepa foram o 2B2, C1, 2B12. A retirada das estruturas de revestimento das sementes de P. notatum foi o pré-tratamento que apresentou o melhor desempenho com 34,5% das sementes geminadas. No teste da sanidade foram identificados os gêneros de fungos Aspergillus, Curvularia, Geniculosporium e Fusarium, e o isolado 2B2 de Trichoderma spp. mostrou eficiente na confrontação direta a estes gêneros de fungos. Para o IVE (índice de velocidade emergência), os tratamentos da combinação do pó biológico do isolado 2B12 de trichoderma e o bioestimulante Stimulate® e somente o bioestimulante Stimulate® com pré-tratamento, e sem pré-tratamento a combinação do pó biológico do isolado 2B2 de trichoderma com bioestimulante Stimulate®, obtiveram os melhores resultados, com 14.48, 13.93 e 12.75, respectivamente. O bioestimulante stimulate®, promove o índice de velocidade de emergência das sementes de P. notatum e a produção de massa seca, a taxa de alongamento inicial da folha (TAIF) interfere positivamente, na massa seca da parte aérea.

Philosophiae Doctor - PhD / During the South African War of 1899-1902 captured civilians were directed by the British army into military controlled zones and into refugee camps which became known as concentration camps. Established near towns, mines and railway sidings these camps were separated along racial lines. The British forced black men, women and children through the violence of war into agricultural and military labour as a war resource, interning over 110,000 black civilians in concentration camps. Unlike Boer civilians who were not compelled to labour, the British forced black civilians into military labour through a policy of no work no food. According to recent scholarly work based only on the written archive, at least 20,000 black civilians died in these camps. This project uses these written archives together with archaeological surveys, excavations, and oral histories to uncover a history of seven such forced labour camps. This approach demonstrates that in constructing an understanding and a history of what happened in the forced labour camps, the written archive alone is limited. Through the work of archaeology which uncovers material evidence on the terrain and the remains of graves one can begin to envisage the scale an extent of the violence that characterized the experience of forced laborers in the 'black concentration camps' in the South African War.

The binary distinction of “native speaker” and “non-native speaker” (NS/NNS) remains the primary way in which professionals are categorised in the field of teaching English to speakers of other languages (TESOL). This distinction is problematic because it is used to place greater value on native English speaking teachers (NEST) over non-native English speaking teachers (NNESTs). This distinction is argued to be largely based on linguistic features (Medgyes 1992; Cook 1999). However the aspect of race remains to be adequately discussed (Kubota and Lin 2006).This thesis has its origins in my personal experiences with racism because, as a Canadian- Filipino, my employer and my students did not accept me as a “real” NEST because I am “non-white”. In my initial research, during my MA TESOL, into the professional experiences of racism I coined the acronym “VEM-NEST”: visible ethnic minority, native English speaking teacher. I used this term to describe the particular group of teachers, to which I belong, who do not easily fit into the available categories of NS/NNS, and consequently NEST/NNEST.My thesis reported on the experiences of nine VEM-NESTs and how they performed specific identities during specific events. Their experiences were presented as individual restoried narratives which were developed from the combination of the participants’ written stories and one-to-one interviews. The restoried narratives were analysed using an analytical lens based on Labov and Waletzky’s (1967) structural approach. The findings suggest that VEM-NESTs need to meet a certain amount of “native speaker” norms in order to be given the opportunity to perform their VEM-NEST role identities in specific situations. This has particular implications for how the NS/NNS binary distinction needs a more nuanced understanding as a way of addressing the inequalities embedded in the way TESOL professionals are valued.