Made available in DSpace on 2014-08-20T13:32:48Z (GMT). No. of bitstreams: 1 tese_thais_farias_collares.pdf: 1094762 bytes, checksum: 647dd933472bf9127c0164ee875617ea (MD5) Previous issue date: 2013-10-17 / The history of sports competition has been related to the use of methods for physical training associated with physiological methods to increase athlete performance. Ethical issues involving the high performance sport and doping are confused with the history of competitive sports. The World Anti - Doping Agency (WADA) prohibits the use of substances or methods to artificially increase sports performance. Currently, 258 substances are listed by WADA and athletes around the world are subjected to tests proving the non-use of doping. EPO is a glycoprotein hormone that has as its main physiological effect induction of erythropoiesis and thereby improving the capacity to transport oxygen in the blood. For these reason EPO has been included in WADA list. The analytical differentiation of endogenous erythropoietin from its recombinant counterpart, using isoelectric focusing and double blotting is a milestone in the detection of doping with recombinant erythropoietin. However, several analogs of the original recombinant EPO are not easily detectable by standard IEF method, requiring the development of alternatives for the detection of doping. In order to improve the current methods of EPO detection, monoclonal and polyclonal antibodies against EPO were obtained and used in various techniques for detection of EPO in biological samples. However, the specificity of these antibodies has been quite controversial and discussed. Therefore, it is necessary to obtain antibodies capable of reacting specifically with the EPO. In this study, rHuEPO was inoculated in New Zealand rabbits to generate a polyclonal antibody (pAb anti-rHuEPO). The pAb was characterized for its potential in detecting rHuEPO using different approaches. The pAb anti- rHuEPO identified the expression of recombinant protein in eukaryotic cells and was able to detect rHuEPO in a suspension at 0.1 μg/mL, showing its potential as a tool for detection of doping by rHuEPO. / A história da competição esportiva sempre esteve relacionada à utilização de metodologias de treinamento físico associadas a métodos de incremento fisiológico do atleta, visando sua máxima performance. Questões éticas envolvendo o esporte de alto rendimento e o doping se confundem com a própria história do esporte competitivo. A Agência Mundial Anti-Doping (WADA) proíbe o uso de substâncias ou métodos capazes de aumentar artificialmente o desempenho esportivo. Atualmente, 258 substâncias estão na lista da WADA e atletas do mundo inteiro são submetidos a testes comprobatórios da não utilização do doping. Entre essas substâncias ilícitas para uso por atletas destaca-se a eritropoetina (EPO). A EPO é um hormônio glicoproteico que possui como principal efeito fisiológico a indução da eritropoiese e consequente melhoria da capacidade de transporte de oxigênio no sangue. A diferenciação analítica da eritropoetina endógena produzida a partir de sua contraparte recombinante usando focalização isoelétrica e duplo blotting é um marco na detecção do doping com eritropoetina recombinante. Anticorpos monoclonais e policlonais específicos anti-EPO foram obtidos e utilizados em várias técnicas para a detecção da EPO em amostras biológicas a fim de melhorar os métodos atuais de detecção. Contudo, a especificidade destes anticorpos tem sido bastante controversa e discutida. Portanto, a obtenção de anticorpos específicos capazes de reagir especificamente com a EPO faz-se necessária. Neste estudo, a rHuEPO foi utilizada para imunizar coelhos New Zealand para gerar um anticorpo policlonal (pAb anti-rHuEPO). O pAb foi caracterizado quanto ao seu potencial na detecção da rHuEPO usando diferentes metodologias. O pAb anti-rHuEPO identificou a expressão da proteína recombinante em células eucarióticas e foi capaz de detectar rHuEPO em suspensão até 0,1 μg/mL, comprovando seu potencial como ferramenta para detecção do doping por rHuEPO.

Francis Picabia, peintre-poète qui a traversé toute la première moitié du XXe siècle, nous a laissé en héritage un riche corpus d’écrits, dont la plupart a été publié, mais il reste encore aujourd’hui de nombreux inédits et des avant-textes conservés dans les archives. Si la période Dada de la poésie de Picabia est peut-être la plus connue, l’artiste a continué d’écrire des poèmes toute sa vie (il est disparu en 1953). C’est notamment après la rencontre avec son dernier éditeur, Pierre André Benoit (PAB), en 1948, que Picabia décide de révéler encore une fois saveine poétique, et publie un grand nombre de recueils, écrits entre 1939 et 1951.Ces poèmes naissent, plus particulièrement, sous l’empreinte des lectures des oeuvres de Friedrich Nietzsche, car l’artiste reprend systématiquement des passages du Gai Savoir, qui nous obligent à repenser les limites de l’écriture poétique, en général. On découvre alors une nouvelle manière de faire de la poésie, qui reprend, d’unemanière assez libre, le langage philosophique de La Gaya Scienza. PAB a sûrement compris la portée des nouveaux poèmes de Picabia et il s’est dédié à la réalisation de solutions créatives pour la présentation de ces écrits poétiques. Au-delà de cet intense travail il y a bien sûr une amitié, qui s’est manifestée notamment à traversl’échange de lettres, car les occasions de se rencontrer ont été très rares.Cette dernière période de l’écriture de Picabia est alors digne d’être remarquée et mérite d’être comprise et mise en valeur. Nous avons essayé, par cette recherche, de trouver une solution pour classer ces écrits, que nous aimerions inscrire dans le contexte de la « récriture intertextuelle ». / Francis Picabia (1879 – 1953), well-known French painter of the beginning of the twentieth century, was also an experimentalist poet of the avant-garde milieu, who wrote throughout his entire life. Part of his writings have been edited, but many unpublished works can still be found in archives. The Dada period of his poetry is perhaps best known, more particularly for his drawing-poems, but I have discovered during my research that his late writings contain many unrevealed aspects. Particularly after he met the editor Pierre André Benoit (PAB) in 1948, a new important period began in Picabia’s poetic output, during which he published his writings from 1939 to 1951. This intense collaboration took place mainly at a distance, through the exchange of frequent letters, soon turning into a sincere friendship. These publications, most of which are one-of-a-kind books, are printed personally by PAB on his printing press, and are often illustrated by the artist or by the editor himself.Surprisingly, Picabia’s late poetry is strongly marked by Friedrich Nietzsche’s writings, in particular by The Gay Science (1882). The “borrowings” are so evident, that they call for a specific, new interpretation: thus, this peculiar creative process can be seen as a phenomenon of appropriation, or “intertextual rewriting”. This process of “copying”, was applied by the artist indistinctively to his art and poetry and can function as a possible solutionfor the classification of his controversial poems.

Orientador: Valeriano Mendes Ferreira Costa / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Filosofia e Ciencias Humanas / Made available in DSpace on 2018-08-13T23:42:44Z (GMT). No. of bitstreams: 1 Seraine_AnaBeatrizMartinsdosSantos_D.pdf: 6208135 bytes, checksum: 6e341e05fbc704d001e8e4da0a734545 (MD5) Previous issue date: 2009 / Resumo: Ao considerar a realidade de pauperização, somada ao aumento do desemprego e ao movimento de desassalariamento, impõe-se ao Estado brasileiro o desafio de promover a inclusão social e, conseqüentemente, reduzir a pobreza. Nesta conjuntura, observa-se a tentativa de o governo federal enfrentar tal realidade socioeconômica quando, a partir da década de 1990, adota como estratégia institucional a Ideologia do Empreendedorismo e, na intenção de dinamizar determinados setores produtivos da economia, com o intuito de minimizar a pobreza, passa a incentivar a criação dos micros e pequenos negócios, estimulando o trabalho por conta própria. Exemplifica essa tentativa o propósito de o governo federal transformar o artesão em empreendedor. O presente trabalho de pesquisa situa-se no campo das Políticas Públicas, destacando aquela destinada ao fomento da produção artesanal - Programa do Artesanato Brasileiro (PAB) - criado em 1991. Face ao exposto, pretende-se analisar o PAB, a partir de sua concepção, institucionalidade e implementação, segundo a contribuição teórica de Bo Rothstein. Toma-se como evidência empírica da execução do PAB, no Piauí, o segmento artesanal ceramista, desenvolvido no Bairro Poty Velho, localizado na zona Norte de Teresina, capital do Estado. / Abstract: Taking into account the reality of impoverishment and social exclusion, together with the increasing of unemployment and with the diminishing of wage earning, it is imposed to the Brazilian State the challenge to promote the social inclusion and, consequently, reduce poverty. It is in this scenario that it is observed the attempt by the Federal Government to face such a socioeconomic reality when, from the Nineties, it adopts the ideology of Enterprising as institutional strategy. Aiming for making certain productive sectors more dynamic, and so minimize poverty, they begin to encourage the opening of micro and small businesses, stimulating autonomous (self governing) work. An example of this attempt is the intention of the Federal Government, in the Nineties, of transforming the artisans into businessmen. The present research work deals with public policies, mainly that one aimed for stimulating craftsmanship production - "Brazilian Craftsmanship Program" (Programa do Artesanato Brasileiro (PAB) established in 1991. What is made in the present work is the analysis of such program from its conception, institutionality and implementation according to the theoretical contribution of Rothstein. It is taken as empirical evidence the execution of PAB in Piauí, the ceramic craftsmanship activity developed in Poty Velho district in the North Zone of Teresina, the capital of the State. / Doutorado / Ciencias Sociais / Doutor em Ciências Sociais

L’objectif de notre étude était de comprendre les difficultés rencontrées par les PAB au cours de la crise de la COVID-19, et aussi comment ces problèmes ont influé leur maintien au travail dans les CHSLD de Montréal du 23 février au 11 juillet 2020. 11 PAB appartenant à trois (3) catégories ont été sélectionnés selon un échantillonnage en boule de neige. Un guide d’entrevue construit sur le modèle de Tourangeau et al. (2010) et celui de la conciliation emploi-famille-vie personnelle proposée par Duxbury et Higgins (2003) a permis de recueillir des données à l’aide d’entrevues semi-dirigées. Elles ont été réalisées entre octobre et décembre 2021 et une analyse thématique des données selon la méthode proposée par Braun et Clarke (2006) a été réalisée. Les résultats de notre étude montrent que l’intention de quitter l’emploi au cours de la période concernée était influencée par le cercle vicieux du bris de service impliquant une surexploitation des PAB, des pressions physiques et psychologiques, un épuisement au travail, un stress aigu ajouté à des émotions négatives. En plus de cela, les mauvaises conditions de travail, les enjeux éthiques spécifiques à la pandémie de la COVID-19, mais également le manque de reconnaissance et de valorisation du métier de PAB, au moment de l’apogée de la pénurie de la main-d’œuvre et les difficultés de conciliation emploi-famille-vie personnelle ont fortement contribué au retrait du travail de certaines PAB. A contrario, l’intention de rester en emploi était plus influencée par des facteurs individuels tels que le volontarisme, la satisfaction morale et l’attachement affectif au métier et des facteurs contextuels tels que le soutien perçu de l’organisation et/ou du gouvernement du Québec. Le réseau des CHSLD initialement fragilisés par la pénurie de main-d’œuvre et la précarisation des emplois de PAB a davantage été affaibli par la crise de la COVID-19 qui a accentué la pénurie de main-d’œuvre. L’amélioration des politiques de rétention des PAB passe par une sélection plus rigoureuse en tenant compte des motivations des candidates au métier, une amélioration des conditions de travail et une meilleure valorisation du métier de PAB. / Our study aims to understand the obstacles encountered by PAB during the COVID-19 crisis and how these influenced their work retention in Montreal's CHSLDs from February 23 to July 11, 2020. Three (3) categories of PAB were selected according to a snowball sampling. An interview guide based on the Tourangeau et al. model (2010) and the work-life balance model proposed by Duxbury and Higgins (2003) was used to collect data through semi-structured interviews conducted between October and December 2021. A thematic analysis was performed using the method suggested by Braun and Clarke (2006). The results of our study show that the intention to leave the job during the period concerned was influenced not only by the vicious circle of service breakdown involving overexploitation of the PAB, physical and psychological pressures, burnout at work, acute stress added to negative emotions. In addition to this, poor working conditions, ethical issues specific to the COVID-19 pandemic, but also the lack of recognition and valuing of the PAB profession at the time of the summit of the workforce shortage and the challenges of reconciling work and family life have enormously contributed to the resignation from the work of some of them. On the other hand, the intention to remain employed was more influenced by individual factors such as voluntarism, moral satisfaction and emotional attachment to the job and contextual factors such as the perceived support of the organization and the Quebec government. The CHSLD network, initially weakened by the labour shortage and the precariousness of PAB jobs was further deteriorated by the COVID-19 crisis, which increased the demand for PAB. The improvement of the retention policies for PAB requires a more rigorous selection process that considers the candidates' motivations for the job, a modification of the working conditions, and a better promotion of the PAB job.

Atelierworkshop is an innovative Wellington based architectural practice that has advanced into the area of off- site manufacture of container-based housing solutions. Their product, the Port-a-Bach (PAB) is moving into its second generation (PAB Gen-2). This product development initiative has resulted in a range of projects that have been undertaken to improve off-the-grid energy systems, water supply and storage, packaging, transportation and cost reduction. This particular project documents the design and development of an energy management and supply accessory product, called the Bach Pack. The Bach Pack seeks to create a viable product energy system solution, at reduced cost and environmental impact (compared with existing solutions) and to achieve this through the development of the usability aspects and features of the product system. The focus is on developing a quality experience for the end user with regards to the attachment and deployment of the components that make up the Bach Pack product. This accessory and modular product solution enables the PAB Gen-2 to be self-sufficient with regard to electrical energy and water supply, and can be specified at point of sale or added later if required. This document focuses explicitly on the design and development of the solar array segment of the Bach Pack.