The generic concept of quasi-single-stage (QSS) power conversion topology for ac-dc rectification and dc-ac inversion is proposed. The topology is reached by direct cascading and synchronized switching of two variety of buck or two variety of boost switching networks. The family of QSS power converters feature single-stage power processing without a dc-link low-pass filter, a unidirectional pulsating dc-link voltage, soft-switching capability with minimal extra commutation circuitry, simple PWM control, and high efficiency and reliability. A new soft-switched single-phase QSS bi-directional inverter/rectifier (charger) topology is derived based on the QSS power conversion concept. A simple active voltage clamp branch is used to clamp the otherwise high transient voltage on the current-fed ac side, and at the same time, to achieve zero-voltage-switching (ZVS) for the switches in the output side bridge. Seamless four-quadrant operation in the inverter mode, and rectifier operation with unity power factor in the charger (rectifier) mode are realized with the proposed uni-polar center-aligned PWM scheme. Single-stage power conversion, standard half-bridge connection of devices, soft-switching for all the power devices, low conduction loss, simple center-aligned PWM control, and high reliability and efficiency are among its salient features. Experimental results on a 3 kVA bi-directional inverter/rectifier prototype validate the reliable operation of the circuit. Other single-phase and three-phase QSS bi-directional inverters/rectifiers can be easily derived as topological extensions of the basic QSS bi-directional inverter/rectifier. A new QSS isolated three-phase zero-voltage/zero-current-switching (ZVZCS) buck PWM rectifier for high-power off-line applications is also proposed. It consists of a three-phase buck bridge switching under zero current and a phase-shift-controlled full-bridge with ZVZCS, while no intermediate dc-link is involved. Input power and displacement factor control, input current shaping, tight output voltage regulation, high-frequency transformer isolation, and soft-switching for all the power devices are realized in a unified single stage. Because of ZVZCS and single-stage power conversion, it can operate at high switching frequency while maintaining reliable operation and achieving higher efficiency than standard two-stage approaches. A family of isolated ZVZCS buck rectifiers are obtained by incorporating various ZVZCS schemes for full-bridge dc-dc converters into the basic QSS isolated buck rectifier topology. Experimental and simulation results substantiate the reliable operation and high efficiency of selected topologies. The concept of charge control (or instantaneous average current control) of three-phase buck PWM rectifiers is introduced. It controls precisely the average input phase currents to track the input phase voltages by sensing and integrating only the dc rail current, realizes six-step PWM, and features simple implementation, fast dynamic response, excellent noise immunity, and is easy to realize with analog circuitry or to integrate. One particular merit of the scheme is its capability to correct any duty-cycle distortion incurred on only one of the two active duty-cycles which often happens in the soft-switched buck rectifiers, another merit is the smooth transition of the input currents between the 60o sectors. Simulation and preliminary experimental results show that smooth operations and high quality sinusoidal input currents in the full line cycle are achieved with the control scheme. / Ph. D.

State-of-the-art silicon-based power supplies have reached a point of maturity in performance. Efficiency, power density, and cost are major trade-offs involved in further improvements. Most products are custom designed with significant non-recurrent engineering and manufacturing processes that are labor intensive. In particular, conventional magnetic components, including transformers and inductors, have largely remained the same for the past five decades. Those large and bulky magnetic components are major roadblocks toward an automated manufacturing process. In addition, there is no specific approach to reduce electromagnetic interference (EMI) in conventional practices. In certain cases, EMI filter design even requires a trial-and-error process. With recent advances in wide-bandgap (WBG) power semiconductor devices, namely, SiC and GaN, we have witnessed significant improvements in efficiency and power density, compared to their silicon counterparts. In a power factor correction (PFC) rectifier/inverter, the totem-pole configuration with critical conduction mode (CRM) operation to realize zero-voltage switching (ZVS) is deemed most desirable for a switching frequency 10 times higher than current practice. With a significantly higher operating frequency, the integration of inductors with embedded windings in the printed circuit board (PCB) is feasible. However, a PCB winding-based inductor has a fundamental limitation in terms of its power handling capability. The winding loss is proportional to the magnetomotive force (MMF), which is Ni. That is to say, with the number of layers (turns) and currents increased, winding loss is increased nonlinearly. Furthermore, for a large-size planar inductor, flux distribution is usually non-uniform, resulting in dramatically increased hysteresis loss and eddy loss. Thus, current designs are challenged by the capability to increase their power range. To address those issues, a modular building block approach is proposed in this dissertation. A planar PCB inductor is formed by an array of pillars that are integrated into one magnetic core, where each pillar handles roughly 750 W of power. The winding loss is reduced by limiting the number of turns for each pillar. The core loss is minimized with a proposed planar magnetic structure where rather uniformly distributed fluxes were observed in the plates. The proposed approach has a similar loss to a conventional litz wire-based design but features a higher power density and can be easily assembled in automation. A 3 kW high frequency PFC converter with 99% efficiency is demonstrated as an example. Furthermore, PCB-based designs up to 6 kW are provided. Another challenge in a WBG-based PFC/inverter is the high common-mode (CM) noises associated with the high dv/dt of the WBG devices. Symmetry and cancellation techniques are often employed to suppress CM noises in switching power converters. Meanwhile, shielding technique has been demonstrated to effectively suppress CM noises in an isolated converter with PCB-based transformer design. However, for non-isolated converters, such as PFC circuits, none of the techniques mentioned above are deemed applicable or justifiable. Recently, the balance technique has been demonstrated to effectively suppress CM noises up to a point where the parasitic ringing between the inductor and its winding capacitor is observed. This dissertation presents an improved balance technique in a PCB-based coupled inductor design that compensates for the detrimental effect of the interwinding capacitors. A CM noise model is established to simplify the convoluted couplings into a decoupled representation so as to illustrate the necessary conditions for realizing a balanced network. In the given 1 kW PFC example, CM noise suppression is effective in the frequency range of interest up to 30 MHz. The parasitic oscillation of inductors, known to be detrimental for CM noise reduction, is circumvented with the improved magnetic structure. By applying the balance technique to a PFC converter and the shielding technique to an LLC DC/DC converter, significant noise reductions were realized. This provides the opportunity to use a simple one-stage EMI filter to achieve the required EMI noise attenuation for a server power supply. This dissertation further offers an in-depth study on reducing the unwanted near-field couplings between the CM/DM inductors and DM filter capacitors, as well as unwanted self-parasitics such as the ESL of the DM capacitors. An exhaustive finite element analysis (FEA) and near field measurements are conducted to better understand the effect of frequency on the polarization of the near field due to the displacement current. The knowledge gained in this study enables one to minimize unwanted mutual coupling effects by means of physical placement of these filter components. Thus, for the first time, a single-stage EMI filter is demonstrated to meet the EMI standard in an off-line 1 kW, 12 V server power supply. With the academic contributions in this dissertation, a PCB winding-based inductor can be successfully applied to a high-frequency PFC/inverter to achieve high efficiency, high power density, automation in manufacturing, lower EMI, and lower cost. Suffice it to say, the proposed approach enables a paradigm shift in the designing and manufacturing of a PFC/inverter for the next generation of power supplies. / Doctor of Philosophy / State-of-the-art silicon device-based switching power supplies have reached a point of maturity in performance. Efficiency, power density, and cost are major trade-offs involved in performance improvements. Most products are custom designed, requiring significant non-recurrent engineering and labor-intensive manufacturing processes. In particular, conventional magnetic components, including transformers and inductors, have largely remained the same for the past five decades. Those large and bulky magnetic components are major roadblocks toward an automated manufacturing process. In addition, there is no specific approach to reduce electromagnetic interference (EMI) in conventional practices. In consequence, a large multi-stage EMI filter is usually adopted between the power converter and the grid to reduce the EMI noise. It generally occupies 1/4-1/3 of the total converter volume. In certain cases, EMI filter design even requires a trial-and-error process. Suffice it to say, EMI is still regarded as both science and art. With recent advances in wide-bandgap (WBG) power semiconductor devices, namely, SiC and GaN, we have witnessed significant improvements in efficiency and power density, compared to their silicon counterparts. With GaN devices, the switching frequency of a PFC converter is able to be increased by 10 times compared to the state-of-the-art design without compromising efficiency. With a significantly higher operating frequency, the integration of inductors with embedded windings in the printed circuit board (PCB) is feasible. However, the state-of-the-art PCB winding-based inductor has a fundamental limitation in power range. Its winding loss and core loss increase dramatically in high powers. To address this issue, a modular building block approach is proposed in this dissertation. A planar PCB inductor is formed by an array of pillars that are integrated into one magnetic core, where each pillar handles roughly 750 W of power. The winding loss is reduced by limiting the number of turns for each pillar. The core loss is minimized with a proposed planar magnetic structure where rather uniformly distributed fluxes have been observed in the magnetic core plates. A 3 kW high-frequency PFC converter with a 99% peak efficiency is demonstrated as an example. Furthermore, PCB-based designs up to 6 kW are provided. Another challenge in a WBG-based PFC/inverter is the high common-mode (CM) noises caused by the high switching speed of the WBG devices. Symmetry and cancellation techniques are often employed to suppress CM noises in switching power converters. Meanwhile, shielding technique has been demonstrated to effectively suppress CM noises in an isolated converter with PCB-based transformer. However, for non-isolated converters, such as PFC circuits, none of the techniques mentioned above are deemed applicable or justifiable. Recently, the balance technique has been demonstrated to effectively suppress CM noises up to several MHz. However, the CM noise reduction is not effective beyond that. This dissertation presents an improved balance technique in a PCB-based coupled inductor to circumvent the limits. In the given 1 kW PFC example, CM noise suppression is effective in the frequency range of interest up to 30 MHz. By applying the balance technique to a PFC converter and the shielding technique to an LLC DC/DC converter, significant noise reductions were realized. This provides the opportunity to use a simple one-stage EMI filter to achieve the required EMI noise attenuation for a server power supply. It features a smaller volume compared to a conventional multi-stage filter. To further enhance the filter's performance at high frequencies, an exhaustive finite element analysis and near field measurements are conducted to better understand the effect of frequency on the polarization of the near field due to the displacement current. The knowledge gained in this study enables one to minimize unwanted mutual coupling effects through physical placement of these filter components. Several approaches for improving the filter performance at high frequency are conducted. With these approaches applied, a single-stage filter is demonstrated in an off-line 1 kW, 12 V server power supply. Thus, for the first time, a single-stage EMI filter can be contemplated to meet the EMI standard in server power supplies. With the academic contributions in this dissertation, a PCB-winding based inductor can be successfully applied to a high-frequency PFC/inverter to achieve high efficiency, high power density, automation in manufacturing, lower EMI, and lower cost. Suffice it to say, the proposed approach in this work enables a paradigm shift in the designing and manufacturing of a PFC/inverter for the next generation of power supplies.

Power electronics building blocks (PEBBs) are standardized building blocks used to integrate power electronics systems. The PEBB approach can achieve low cost, high redundancy, high reliability, high flexibility and easy maintenance for large-scale power electronics systems. This thesis presents the implementation of a 100kW PEBB-based soft-switched bus regulator for an 800V DC distributed power system. The zero current transition (ZCT) soft-switching technique is used to improve the performance of the bus regulator by minimizing switching loss and improving overall efficiency. PEBB modules and a digital control building block are the subsystems of the DC bus regulator. This thesis addresses the design issues at subsystem and system levels. These include: operational principles and design of ZCT PEBB modules; design and implementation of the digital control block, based on DSP and EPLD; and modeling and control design of the DC bus regulator. There are several considerations when using the ZCT soft-switching technique in three-phase applications: the timing of the auxiliary switch gate signals must be arranged differently; there are low-frequency harmonics caused by the pulse width limits; and there is high thermal stress on the resonant capacitors. These issues are resolved by utilizing the sensed phase current information and the design freedom in the PWM modulator. A PWM modulation technique is proposed that can considerably reduce the switching events and further remove the associated loss while keeping THD low. Reduced switching events alleviate the thermal issue of the resonant capacitors. The same modulation technique can avoid the low-frequency harmonics caused by the pulse width limits and double the sampling frequency. The phase current information is used to deal with the control timing issue of the auxiliary switches and to control the three-phase soft-switching operation in order to achieve better efficiency. Additionally, the phase current information is used to implement dead time compensation to reduce THD. The soft-switched DC bus regulator has been tested up to a 100kW power level with 20kHz switching frequency. Experimental results demonstrate that high performance of the DC bus regulator is accomplished in terms of wide control bandwidth, low THD, unity power factor, high efficiency and high power density. / Master of Science

BACKGROUND:The widespread utilization of organic compounds in modern society and their dispersion through wastewater have resulted in extensive contamination of source and drinking waters. The vast majority of these compounds are not regulated in wastewater outfalls or in drinking water while trace amounts of certain compounds can impact aquatic wildlife. Hence it is prudent to monitor these contaminants in water sources until sufficient toxicological data relevant to humans becomes available. A method was developed for the analysis of 36 trace organic contaminants (TOrCs) including pharmaceuticals, pesticides, steroid hormones (androgens, progestins, and glucocorticoids), personal care products and polyfluorinated compounds (PFCs) using a single solid phase extraction (SPE) technique with ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). The method was applied to a variety of water matrices to demonstrate method performance and reliability.RESULTS:UHPLC-MS/MS in both positive and negative electrospray ionization (ESI) modes was employed to achieve optimum sensitivity while reducing sample analysis time (<20min) compared with previously published methods. The detection limits for most compounds was lower than 1.0 picogram on the column while reporting limits in water ranged from 0.1 to 15ng/L based on the extraction of a 1L sample and concentration to 1mL. Recoveries in ultrapure water for most compounds were between 90-110%, while recoveries in surface water and wastewater were in the range of 39-121% and 38-141% respectively. The analytical method was successfully applied to analyze samples across several different water matrices including wastewater, groundwater, surface water and drinking water at different stages of the treatment. Among several compounds detected in wastewater, sucralose and TCPP showed the highest concentrations.CONCLUSION:The proposed method is sensitive, rapid and robust / hence it can be used to analyze a large variety of trace organic compounds in different water matrixes.

Le contrôle précis des boucles de régulation existantes sur une machine frigorifique est essentiel à son bon fonctionnement. Il existe plusieurs méthodes de régulation parmi lesquelles on retrouve la plus ancienne et la plus connue : la commande PID (Proportionnelle, Intégrale et Dérivée). Malgré la simplicité apparente des relations mises en jeu, le régulateur PID est assez délicat à ajuster sur des processus présentant de fortes perturbations comme les installations frigorifiques. L’objectif du travail présenté est de mettre en œuvre une commande prédictive fonctionnelle (PFC) afin de réguler la surchauffe en sortie d’évaporateur, la pression de condensation et la puissance frigorifique sur une installation munie d’un compresseur à vitesse variable. L’utilisation d’une commande PFC nécessite de réaliser une prédiction de l’évolution à venir de la sortie du procédé. C’est un modèle interne qui fait office de modèle de connaissance. En assimilant le procédé à un système du premier ordre, la mise en œuvre de cette commande ne nécessite que trois paramètres : un gain statique, une constante de temps et un retard pur. L’originalité de la démarche développée a consisté à réaliser une modélisation physique de la machine afin de déterminer les paramètres précédents par une approche macroscopique. Une bibliothèque de modèles physiques a été élaborée permettant de simuler le comportement de différents évaporateurs, condenseurs, compresseurs ou vannes de détente. La commande développée a ensuite été implantée sur un automate industriel programmable et des expériences ont été réalisées sur deux machines différentes. La première est constituée de deux échangeurs à tubes et calandre et d’un compresseur à pistons, alors que la seconde comprend un évaporateur à plaques, une batterie à ailettes et un compresseur à vis. / An accurate control of fluids flow is essential in any refrigeration system. As the conventional Proportional-Integral-Derivative (PID) control with invariable parameters can lead to unsatisfactory performance because of the variation of refrigeration unit parameters under disturbances, the aim of the work presented here is to develop a method for using Predictive Functional Control (PFC) to regulate the evaporator superheat, the condensing pressure and the cooling capacity on a variable-speed refrigeration system. Using a predictive controller requires to predic future change at the output of the process. This prediction is based on an internal model used as known model. By assuming that the behaviour of refrigerating machine heat exchangers can be represented using a first-order model, the implementation of PFC requires only three parameters: gain, time constant and time delay. In order to determine these parameters an original method has been developed which is based on the physical modelling of the machine. Physical models for different types of evaporator, condenser, compressor or expansion valve have been established to quantify heat transfer and refrigerant flow rate in these components. The control system created has been incorporated into an industrial programmable logic controller and used for experiments on two different refrigerating machines: the first one is composed of two shell and tube heat exchangers and a reciprocating compressor, whereas the second one is composed of a plate evaporator, a finned-tube condenser and a screw compressor. The tests performed show that PFC controller succeed in maintaining a precise chilled liquid temperature.

Ce travail s’oriente vers la valorisation du traitement d’eaux usées à partir de piles à combustibles microbiennes (PCM) benthiques pour la production d’électricité. Cette technologie permet la production d’électricité à partir de micro-organismes électro-actifs (EA) et d’un substrat carboné qui peut être de l’eau usée.Quatre types d’eaux usées issues de l’activité anthropique sont sélectionnés. La présence de micro-organismes EA est mise en évidence par 2 méthodes électrochimiques. En condition réelle, le milieu lagunaire présente les meilleures performances électriques (6,6 mW/m²). Celui-ci propose l’environnement le plus favorable à l’installation dePCM benthiques in-situ. Les résultats montrent une forte influence des micro-algues sur l’activité des biofilms EA. Avec un cycle jour/nuit, cette production varie en suivant les cycles d’éclairage. Les micro-algues apportent l’oxygène nécessaire aux réactions cathodiques. Les PCM améliorent également la consommation de polluants du milieu cathodique.L’alimentation de petits dispositifs tels que des capteurs passe obligatoirement par une augmentation de la tension délivrée par les biopiles. Différentes techniques d’élévation de la tension (mise en série et en parallèle de plusieurs piles, convertisseurs DC/DC) sont analysées. Un capteur de température et d’humidité a fonctionné durant plus de 15 h directement alimenté par une de nos biopiles benthiques avec une puissance de 328 µW. Sa tension de sortie est augmentée par un convertisseur de type Flyback, passant de 560 mV à plus de 5,5 V. L’utilisation de PCM in-situ dans la lagune peut constituer une alternative à la production électrique et au traitement des eaux usées. / The work described in this document is oriented to enhancing the treatment of wastewater from benthic microbial fuel cell (BMFC) for electricity production. This technology allows the production of electricity from electro-active (EA) microorganisms and carbonated substrate which may be the wastewater.Four types of wastewater from human activity are selected. The presence of EA microorganisms is highlighted by two electrochemical methods. In real conditions, the lagoon environment has the best electrical performance (6.6 mW/m²).The lagoon environment offers the most favorable environment for installation BMFC in-situ. The results show a strong influence of microalgae on the EA biofilms activities and thus on the production of electricity. In lagoon conditions, with a day/night cycle, this production varies according to the lighting cycles. Microalgae bring oxygen necessary for cathode reactions at lower cost. BMFC also improve the consumption of pollutants including organics.Electrical supply by small devices such as sensors necessarily requires an increase of the voltage delivered by BMFC. The different voltage boosting techniques such as series and parallel connections of several units or the use of DC/DC converters are performed and analyzed. A temperature and humidity sensor worked for more than fifteen hours directly powered by a BMFC with a power of 328 µW. Its output voltage is increased by a flyback type DC/DC converter, from 560 mV to more than 5.5 V. The use of PCM in-situ in the lagoon can be an alternative to the power generation and the treatment of wastewater.

Este trabalho propÃe o estudo de uma nova topologia, com dois estÃgios, de um conversor estÃtico, onde existe um fluxo de potÃncia auxiliar com o objetivo de aumentar o rendimento do sistema. O primeiro estÃgio à um conversor CA-CC com correÃÃo do fator de potÃncia (PFC) e o segundo estÃgio à um conversor CC-CC isolado em alta frequÃncia. Os dois estÃgios do conversor proposto sÃo modelados por equaÃÃes diferenciais e atravÃs desses modelos sÃo desenvolvidas tÃcnicas de controle nÃo linear para o funcionamento dos conversores em malha fechada. A correÃÃo do fator de potÃncia do primeiro estÃgio à realizada pela tÃcnica de controle PBC (passivity-based control), enquanto que a tensÃo de saÃda do primeiro estÃgio à realizada pelo controle I&I (immersion and invariance). O segundo estÃgio necessita controlar somente a tensÃo de saÃda atravÃs do controle backstepping, por se tratar de um conversor CC-CC. / This work proposes a study of a new static converter topology with two stages, where the first is an AC-DC converter with PFC and the second is a DC-DC converter isolated in high-frequency. In addition, the static converter has a secondary power flow to achieve a better efficiency from the system. The two converterâs stages are modeled as differential equations, and through those models nonlinear control techniques are developed for close loop operation. The power-factor correction in the first stage is performed by the PBC (passivity-based control) control technique, while the output voltage from the first stage is performed by the I&I (immersion and invariance) control. As the second stage is a DC-DC converter, it only needs to control the output voltage, which is achieved through the backstepping control.

Les travaux présentés dans ce mémoire s'inscrivent dans une thématique nouvelle pour 1*équipe Electronique de Puissance du LEG : "l'intégration hybride des composants passifs". Us portent sur la conception de composants passifs et s'attardent sur leur modélisation, leur réalisation et leur caractensation. Ces composants seront employés au sein d'un convertisseur AC-DC à prélèvement sinusoïdal qui servira de support à notre étude. Il ressort d'une évaluation de quelques convertisseurs que la structure à résonance série se prête bien à l'intégration. Les effets des interactions électriques en haute fréquence ont été pris en compte grâce à une nouvelle formulation axisymétrique. Cette dernière couplée avec les équations de circuit électrique s'avère bien adaptée pour la caractérisation électromagnétique des composants magnétiques. Les travaux de modélisation des composants passifs, développés au LEG, ont permis de caractériser la structure LCT en prenant en compte tous les aspects utiles de son comportement. Cela a conduit, en particulier, à l'évaluation des pertes dues au diélectrique. Les mesures ont montré que la structure LCT intégrée apporte surtout une meilleure compacité.

Perfluoroalkyl compounds (PFCs) are a group of chemical surfactants most notably used in non-stick and stain-resistance applications. Due to their wide-spread use and inherent resistance to degradation, several PFCs have become persistent environmental contaminants. Despite the high concentrations of PFCs reported in wild birds and their eggs, very little is known about the toxicological effects they have on avian species. This thesis investigates the developmental toxicity of PFCs in an avian model species: the domestic chicken (Gallus gallus domesticus). Egg injection experiments were performed to assess the in ovo toxicity of perfluorooctane sulfonate (technical grade, T-PFOS), perfluorooctanoic acid (PFOA), perfluorodecane sulfonate (PFDS) and perfluoroundecanoic acid (PFUdA). Real-time RT-PCR was then used to measure the transcription of candidate biomarker genes in the liver tissue of day 20 embryos. Candidate genes were selected based on their responsiveness to PFC exposure in previously conducted in vitro screening assays. In ovo exposure to PFOS resulted in a dose-dependent decrease in embryo pipping success (a measure of hatching success) with an LD50 of 93 μg/g (3.54 μg/g-672,910 μg/g, 95% confidence interval), however the expression of peroxisome proliferator-activated receptor alpha (PPARα)-regulated genes was not affected in liver tissue as hypothesized. PFOA, PFDS and PFUdA had no effect on the pipping success of chicken embryos. The expression of cytochrome P450 1A4 (CYP1A4) and liver fatty acid binding protein (L-FABP) mRNA increased in embryo liver tissue following in ovo exposure to PFUdA but was only statistically significant at 10 μg/g, which is several orders of magnitude higher than concentrations reported in wild bird eggs. The isomer-specific accumulation of PFOS in chicken embryo livers was also investigated using an in-port derivatization gas-chromatography/mass spectrometry (GC-MS) method. Prior to incubation, chicken eggs were injected with T-PFOS, composed of 63% linear isomer (L-PFOS) and 37.3% branched isomers. The isomer profiles in day-20 embryo liver tissue showed up to 20% enrichment in the proportion of L-PFOS, compared to T-PFOS, with a corresponding decrease in the proportion of branched isomers. This enrichment was inversely proportional to dose. Finally, the transcriptional profiles of cultured chicken embryonic hepatocytes (CEH) exposed to either T-PFOS or L-PFOS were compared using Agilent 4x44k Chicken (V2) Gene Expression microarrays. At equal concentrations (10 μM), T-PFOS altered the expression of significantly more genes (340 genes, >1.5 fold change, false discovery rate adjusted p<0.05) compared to L-PFOS (130 genes). Functional analysis showed that L-PFOS and T-PFOS affected genes involved in lipid metabolism, cellular growth and proliferation, and cell-cell signaling. Pathway and interactome analysis suggested that gene expression may be affected through RXR, oxidative stress response, TP53 signaling, MYC signaling, Wnt/β-catenin signaling and PPARγ and SREBP receptors. In all functional categories and pathways examined, T-PFOS had a more pronounced disruptive effect on transctional regulation than L-PFOS. In summary, egg injection experiments showed that T-PFOS (but not linear PFOA, PFDS or PFUdA) may affect the hatching success of the chicken at environmentally relevant concentrations. It was also demonstrated that the accumulation of PFOS in embryonic liver is isomer specific, and leads to an enrichment of L-PFOS. The increased transcriptional disruption caused by T-PFOS in cultured hepatocytes over L-PFOS suggests that the branched isomers may be largely responsible for the toxicological effects of PFOS. Combined, the results from this thesis demonstrate the importance of considering PFOS isomer burdens during risk assessment. In addition, gene expression analysis identified several candidate mechanisms for PFOS toxicity.

Perfluoroalkyl compounds (PFCs) are a group of chemical surfactants most notably used in non-stick and stain-resistance applications. Due to their wide-spread use and inherent resistance to degradation, several PFCs have become persistent environmental contaminants. Despite the high concentrations of PFCs reported in wild birds and their eggs, very little is known about the toxicological effects they have on avian species. This thesis investigates the developmental toxicity of PFCs in an avian model species: the domestic chicken (Gallus gallus domesticus). Egg injection experiments were performed to assess the in ovo toxicity of perfluorooctane sulfonate (technical grade, T-PFOS), perfluorooctanoic acid (PFOA), perfluorodecane sulfonate (PFDS) and perfluoroundecanoic acid (PFUdA). Real-time RT-PCR was then used to measure the transcription of candidate biomarker genes in the liver tissue of day 20 embryos. Candidate genes were selected based on their responsiveness to PFC exposure in previously conducted in vitro screening assays. In ovo exposure to PFOS resulted in a dose-dependent decrease in embryo pipping success (a measure of hatching success) with an LD50 of 93 μg/g (3.54 μg/g-672,910 μg/g, 95% confidence interval), however the expression of peroxisome proliferator-activated receptor alpha (PPARα)-regulated genes was not affected in liver tissue as hypothesized. PFOA, PFDS and PFUdA had no effect on the pipping success of chicken embryos. The expression of cytochrome P450 1A4 (CYP1A4) and liver fatty acid binding protein (L-FABP) mRNA increased in embryo liver tissue following in ovo exposure to PFUdA but was only statistically significant at 10 μg/g, which is several orders of magnitude higher than concentrations reported in wild bird eggs. The isomer-specific accumulation of PFOS in chicken embryo livers was also investigated using an in-port derivatization gas-chromatography/mass spectrometry (GC-MS) method. Prior to incubation, chicken eggs were injected with T-PFOS, composed of 63% linear isomer (L-PFOS) and 37.3% branched isomers. The isomer profiles in day-20 embryo liver tissue showed up to 20% enrichment in the proportion of L-PFOS, compared to T-PFOS, with a corresponding decrease in the proportion of branched isomers. This enrichment was inversely proportional to dose. Finally, the transcriptional profiles of cultured chicken embryonic hepatocytes (CEH) exposed to either T-PFOS or L-PFOS were compared using Agilent 4x44k Chicken (V2) Gene Expression microarrays. At equal concentrations (10 μM), T-PFOS altered the expression of significantly more genes (340 genes, >1.5 fold change, false discovery rate adjusted p<0.05) compared to L-PFOS (130 genes). Functional analysis showed that L-PFOS and T-PFOS affected genes involved in lipid metabolism, cellular growth and proliferation, and cell-cell signaling. Pathway and interactome analysis suggested that gene expression may be affected through RXR, oxidative stress response, TP53 signaling, MYC signaling, Wnt/β-catenin signaling and PPARγ and SREBP receptors. In all functional categories and pathways examined, T-PFOS had a more pronounced disruptive effect on transctional regulation than L-PFOS. In summary, egg injection experiments showed that T-PFOS (but not linear PFOA, PFDS or PFUdA) may affect the hatching success of the chicken at environmentally relevant concentrations. It was also demonstrated that the accumulation of PFOS in embryonic liver is isomer specific, and leads to an enrichment of L-PFOS. The increased transcriptional disruption caused by T-PFOS in cultured hepatocytes over L-PFOS suggests that the branched isomers may be largely responsible for the toxicological effects of PFOS. Combined, the results from this thesis demonstrate the importance of considering PFOS isomer burdens during risk assessment. In addition, gene expression analysis identified several candidate mechanisms for PFOS toxicity.