Yes / A central challenge in microbial community ecology is the delineation of appropriate units of biodiversity, which can be taxonomic, phylogenetic, or functional in nature. The term ‘community’ is applied ambiguously; in some cases, the term refers simply to a set of observed entities, while in other cases, it requires that these entities interact with one another. Microorganisms can rapidly gain and lose genes, potentially decoupling community roles from taxonomic and phylogenetic groupings. Trait-based approaches offer a useful alternative, but many traits can be defined based on gene functions, metabolic modules, and genomic properties, and the optimal set of traits to choose is often not obvious. An analysis that considers taxon assignment and traits in concert may be ideal, with the strengths of each approach offsetting the weaknesses of the other. Individual genes also merit consideration as entities in an ecological analysis, with characteristics such as diversity, turnover, and interactions modeled using genes rather than organisms as entities. We identify some promising avenues of research that are likely to yield a deeper understanding of microbial communities that shift from observation-based questions of ‘Who is there?’ and ‘What are they doing?’ to the mechanistically driven question of ‘How will they respond?’

nÃo hà / A prÃtica drag queen diz respeito a um processo de adequaÃÃo que desloca a aparÃncia corriqueira de uma pessoa para uma outra que pode transitar entre gÃneros (masculino, feminino, polimorfo, diversos) e espÃcies (humano e animal, como à o caso de drags que se apresentam como animais e atà alienÃgenas). O presente trabalho tem por objetivo observar e participar dessa prÃtica na cidade de Fortaleza â CE com intuito de absorver seus processos de criaÃÃo, adaptaÃÃo, montagem e desmontagem. Entender as mutaÃÃes as quais essa prÃtica està sujeita, as influÃncias externas e a capacidade de adaptaÃÃo das drag queens estudadas. Usar lentes analÃticas que possibilitem enxergar as prÃticas atravÃs de uma epistemologia da performance que leve em consideraÃÃo a decolonizaÃÃo do pensamento e a apreensÃo crÃtica da tradiÃÃo cientÃfica europeia. O estudo se configura por meio de uma experiÃncia etnogrÃfica embasada no exercÃcio da descriÃÃo densa e de entrevistas pontuais. Os registros foram feitos mediante diÃrio de campo, fotografias, vÃdeos e gravador de voz. Concluiu-se que a prÃtica drag queen na cidade de Fortaleza â CE passou e passa por mudanÃas constantes no que diz respeito à tradiÃÃo e ao surgimento de novas formas de fazer drag. Formas essas impactadas pelo reality show americano RuPaulâs Drag Race e sua tendÃncia de transformaÃÃo da drag queen em um produto passÃvel de ser comercializado mundialmente atravÃs da TV. Observou-se ainda que, ademais da forte influÃncia trazida por esse reality show, o contexto local tem se mostrado resistente Ãs tentativas de suplantaÃÃo da tradiÃÃo, tendo como elementos de resistÃncia o bate- cabelo e o dialeto yorubà que se contrapÃem à forÃa histÃrica de opressÃo conhecida como colonizaÃÃo.

Social insects are the most dominant terrestrial fauna for the last 50 million years. This tremendous ecological success is accompanied by the fact that sociality has evolved multiple times independently and achieved highest degree of complexity in insect lineages. The remarkable social organization found in insect societies is the result of finely balanced cooperation and conflict among the colony members. A typical hymenopteran colony is characterised by one or a few queens monopolizing reproduction and several sterile workers co-operatively raising brood and performing colony activities. The colonies are often conceptualized as superorganisms where groups of cooperative workers are compared with organs in the body, each of which accomplish a particular task like brood care, foraging and defence. The choice of tasks is often regulated by a systematic age polyethism. As the queens monopolize reproduction, they serve as the sole suppliers of eggs in the colony. Therefore, loss or death of the queen creates a crucial void which exposes the colony to potential reproductive conflict for the position of egg-layer. This crisis is expected to be extreme in monogynous colonies. The situation is rescued only after a new queen is established, and the whole process is known as queen succession. I am interested in this crisis management, and my thesis deals with potential and realized conflicts associated with queen succession and behavioural strategies involved in resolution of these conflicts. The queen can be replaced in two ways - either by a newly eclosed specialized reproductive individual, which happens in highly eusocial hymenopterans, or by an existing member of the colony (worker), as it happens in primitively eusocial hymenopterans. Unlike in highly eusocial species, the workers of primitively eusocial species retain their ancestral capability of mating and activating ovaries to produce both sons and daughters, which makes them suitable for taking up the role of queen in their lifetime. Hence, primitively eusocial species provide a unique situation where loss or death of the queen might result in severe reproductive conflict as the queen can be replaced by any one of the existing workers. Strictly monogynous colonies of the tropical primitively eusocial wasp Ropalidia marginata provide ideal opportunities to study the reproductive conflict and its resolution associated with queen succession because the queen is frequently replaced by one of her nestmates resulting in a serial polygyny. These queens have highly variable tenures of queenship ranging from seven to over 200 days, which, together with the fact that they are replaced by a variety of relatives such as daughters, niece and cousins, suggests a potential reproductive conflict with variable degrees of complexity. I have divided my thesis in three parts which are as follows -Natural queen turnover: Previous works from this lab have tried to characterize the queen succession in R. marginata colonies by experimentally removing the queen from the colony. As this design involves the experimenter intervening at a random point of the colony cycle, the colony might not respond in the similar way as it might have done to a natural succession necessitated by loss or death of the queen. But rarity and unpredictability of natural queen turnovers made them difficult to study. Therefore, in this section, we gathered a dataset of long-term and opportunistic quantitative behavioural observations on eleven natural queen turnovers and compared them with available data on queen removal experiments. All our queen removal experiments resulted in a hyper-aggressive potential queen who gradually reduced her aggression, activated her ovaries and went on to become the unanimously accepted new queen of the colony if the original queen was not returned. Here we found a similar phenomenon in natural colonies where a single un-challenged potential queen took over the colony as new queen after the old queen was lost, died or was driven out of the colony. In some of the natural colonies, the transition was preceded by aggression shown by the potential queens towards their nestmates including the queens, which indicates that they might have pre-empted the transition. The potential queens in natural colonies started laying eggs much faster than in experimental colonies suggesting their physiological readiness for the transition. How does a colony respond to a declining queen?: As we could show that some of the potential queens might perceive the upcoming queen turnover, a fair prediction would be that they sense it through the declining fertility status of the queens. Therefore, we tried to ex-perimentally induce situations where the queen appears to be declining, expecting that it might lead to a queen turnover. The growing evidence suggests that R. marginata queen maintains her status by applying a pheromone on the nest surface by rubbing the tip of her abdomen. We knocked down the nest to deny the queen the surface for applying her pheromone, and argued that the queen would be overthrown as the workers would sense her as infertile. To our surprise, the queen maintained her status in six out of seven colonies by applying her pheromone on the entire surface of the cage. However, the effectively insufficient concentration of pheromone elicited aggression from workers towards the queen, and the queen retaliated back with aggression. These results suggest that the pheromone, being an honest signal of fertility, is extremely important for the queen for maintaining her reproductive monopoly, and the workers are able to perceive the decline of the queen from her pheromone. Queen-successor conflict over access to reproduction: Here we more explicitly looked at the potential reproductive conflict between the queen and her successor over access to direct reproduction. We used the theory of parent-offspring conflict proposed by Robert Trivers (1974) as the conceptual framework and adapted it to unravel the pat-tern of queen-successor conflict in R. marginata colonies. According to this idea, we expected that there should be a pre-conflict zone where the queen and the successor both would agree that the queen should continue to reproduce, followed by a conflict zone where the successor would try to takeover but the queen would hang on, finally followed by post-conflict zone where they both would agree that the successor should reproduce. To test this expectation, we maintained the queen and the potential queen on either side of a wire-mesh partition, each with randomly chosen half of the workers. It allowed the potential queen (successor) to establish herself and then we reintroduced the queen to her side of the mesh daily till the queen gave up. We could behaviourally characterise all three zones which always appeared in the expected sequence. The pre and post-conflict zones had no aggressive interaction between the queen and the potential queen, whereas the conflict zone was characterized by aggressive falling fight between them. This is our first success in experimentally creating overt conflict between the queen and her successor. Overall we can say, that the queens and the potential queens of R. marginata show great behvioural plasticity which might have been shaped by natural selection as an adaptation for conflict resolution. We could show that the potential queens sometimes can predict the upcoming transition and pre-pare themselves accordingly, whereas they can also react to an experimentally created sudden loss of queen by hugely elevating their aggression. The docile queens, on the other hand, maintain their reproductive monopoly by a pheromone, which is essentially a feature of highly eusocial species. But these docile queens have not lost their capability to show aggression and can use that to complement the insufficient concentration of her pheromone. This and the behaviour of potential queens in their establishment phase are strongly reminiscent of typical primitively eusocial species. We conclude that Ropalidia marginata is, perhaps, a particularly advanced primitively eusocial hymenopteran situated on an evolutionary continuum from primitive to highly eusocial species.

Thesis (MBA)--University of Stellenbosch, 2010. / Although several women's leadership studies globally have identified a number of issues that women grapple with in their upward mobility in building a career, including gender differences as these relates to styles of leadership, and the behaviour of senior women toward other women in organisations, there has however not been a South African study documenting the existence of the Queen Bee syndrome within Multi-National Corporates, as a barrier to professional women's career growth. The "queen bee syndrome" is a term that has been used to describe women managers who find themselves within a predominantly male environment and have alienated other women by their behaviour, and are therefore perceived to be a barrier to the advancement of other women coming through the ranks. These women fail to assist other women in their aspirations to advance and do not work towards the advancement and empowerment of other women within the corporate sector. The queen bees are normally very protective of their powerbase and attempt to surround themselves with men. They do not believe in female solidarity behaviour and do not actively seek to mentor or coach other women. The aim of the present study is to investigate the existence of the queen bee syndrome in South African retail banks. The following aspects, as they relate to the queen bee syndrome, are examined: • The establishment of the existence of the queen bee syndrome. • The reason for the existence of queen bees. • The possible implication of the existence of queen bee behaviour for the organisations. A total of 25 women in executive and senior management positions, from South Africa's 5 retail banks, were interviewed for the present study. These women were asked to relate their unique personal experiences and perceptions of queen bee behaviour. The method of Content analysis was used to analyse the responses received through the interviews. The findings of the present study clearly indicated that women still face barriers to advancement within corporate organisations, and that organisations need to make adjustments to their internal cultures, structure and strategy to accommodate the growing workforce of females who are rapidly joining the management ranks. The blame for women's lack of progress into senior positions cannot be generally attributed to males only, as women also need to work at growing, developing, educating and empowering themselves in order to facilitate their own advancement, besides helping, supporting and guiding other women in their career progressions. The present study also found that senior women managers and women executives work actively towards being coaches and mentors to women coming through the ranks and assist them in their development towards management roles. The present study's findings indicated that senior women executives and managers currently support women development agendas within their organisations, and also that the presence of the queen bees did not serve as a stumbling block to the advancement of the sample of women who took part in this study. The study was limited to 5 retail banks of South Africa only. Future studies can investigate other sectors and work at providing tools that will assist women in combating the behaviour of the queen bee. It might also be useful to determine whether women are more supportive or less supportive of other women, and women's issues and programs.

Many bacteria, viruses and other parasites cause severe morbidity or mortality in their host populations, creating strong selection for physiological or behavioural mechanisms to avoid disease. Likewise, changes in host susceptibility and contact patterns can dramatically influence the spread of infectious diseases, and hence selection for traits such as virulence and infectivity range in parasites. Understanding how ecological and evolutionary changes in one population affect selection in another represents a key challenge for theoreticians and empiricists alike, and is essential for gaining further insights into host-parasite relationships. This thesis contains theoretical models that explore how genetic and environmental factors shape the evolutionary and coevolutionary dynamics of hosts and parasites. In particular, the roles of genetic specificity (i.e. genotype-by-genotype interactions) and population mixing patterns are investigated, using both mathematical models and computer simulations. A broad range of scenarios are covered, including the coevolution of broad resistance and infectivity ranges (generalism), the persistence of coevolutionary cycling and the maintenance of sex, the effects of mating behaviour on disease prevalence and evolution, and the evolution of sexual and social behaviour. The models presented herein develop our understanding of host-parasite relationships and highlight the importance of genetic interactions and ecological feedbacks.

O objetivo geral desta dissertação é testar hipóteses relacionadas à duração das linhagens (i.e. o intervalo de tempo definido pela origem e extinção das diferentes linhagens) e à dinâmica da diversificação dos ruminantes. Para isso, nós usamos compilações públicas de dados fósseis e modelos probabilísticos. Como a literatura macroevolutiva se aproveita das múltiplas escalas taxonômicas onde é possível estudar a mudança da biodiversidade no tempo, os dois capítulos desta dissertação testam suas hipóteses no níveis de espécie e gênero na tentativa de iluminar o papel da escala na manifestação dos padrões macroevolutivos. No primeiro capítulo, nós investigamos se tratamentos taxonômicos distintos aplicados aos dados brutos alteram os resultados obtidos por um método macroevolutivo. Nossos resultados sugerem que os dados, mesmo após passar por diferentes tratamentos, indicam similares dinâmicas de diversificação tanto no nível taxonômico dos gêneros quanto no nível das espécies. Por outro lado, as comparações entre dois conjuntos de dados inicialmente distintos podem gerar diferenças consideráveis na dinâmica da diversificação e inclusive indicar diferenças entre escalas taxonômicas. Esses resultados são robustos mesmo que as imperfeições do registro fóssil sejam consideradas de diferentes maneiras pelo método usado. No segundo capítulo, nós usamos modelos probabilísticos para testar uma importante hipótese macroevolutiva que é também considerada uma premissa para vários estudos da área: a de que a probabilidade de extinção de qualquer linhagem é independente da sua longevidade, ou seja, do intervalo de tempo que a linhagem já viveu. Nossos resultados sugerem um padrão consistente de diminuição na probabilidade de extinção conforme as espécies vivem por mais tempo, enquanto que o padrão observado do no nível dos gêneros é menos claro. Este resultado não só enriquece o nosso conhecimento a respeito da dinâmica da extinção de linhagens, como sugere que a premissa de independência de idade na probabilidade de extinção (importante para vários estudos macroevolutivos) pode não ser válida, pelo menos para Ruminantia. Nós esperamos que os dados e ideias aqui expostos interajam com novas ideias e dados do leitor, estimulando o desenvolvimento da macroevolução / The main goal of this dissertation is to test hypotheses related to the duration of the lineages (i.e. the time interval between the origin and extinction of each lineage) and to the diversification dynamics of the ruminants. To achieve this goal, we used public compilations of fossil data and probabilistic models. As the macroevolutionary literature take advantage of multiple taxonomic scales in which is possible to study biodiversity changes through time, the two chapters of this dissertation tested different hypotheses in both the species and genus levels, as na attempt to shed some light on the role of scale influencing the macroevolutionary phenomena. In the first chapter, we investigated if different taxonomic treatments applied over the raw data significantly change the results obtained from a macroevolutionary method. Our results suggest that the same data, even passing through different data curations, indicate similar diversification dynamics in species and genus taxonomic levels. On the other hand, data that comes from different sources may present considerable differences in the diversification dynamics, and even indicate differences between taxonomic scales. These results are robust to different considerations of the preservation biases of the fossil record by the method. In the second chapter, we used probabilistic models to test an importante macroevolutionary hypothesis that is also considered a premise of several macroevolutionary studies: that the probability of extinction of any lineage is independent of its longevity (the time span determined by the origination and extinction of each lineage). Our results suggest a consistent pattern of decreasing extinction probability as species live for longs periods of time, but the pattern presented by the genera is less clear. These results not only enhance our knowledge about extinction dynamics, but it also suggests that the premise of age independence in the extinction probability (important for several macroevolutionary studies) might not be valid, at least for Ruminatia. We hope our data and ideas interact with new ideas and data from the reader to stimulate the development of the macroevolutionary field

A pesquisa que intitulamos - Ektheósis Arsinoes: o culto de Arsinoe II Filadelfo, tem como objetivo analisar as razões, formato e alcance do culto religioso criado por Ptolomeu II Filadelfo para honrar a sua irmã-esposa. Sabe-se que a visão que os autores antigos e estudiosos modernos têm a respeito da rainha Arsinoe II era controversa no passado e continua a ser nos dias atuais. Da rainha ambiciosa a esposa devotada, poucos documentos existem sobre a sua passagem no Egito na década de 280/270 a.C. quando ela se tornou rainha durante o governo de seu irmão, embora a grande maioria dos testemunhos data do período que se segue à sua morte. Quem foi Arsinoe II Filadelfo? Por que Ptolomeu II estabeleceu um culto em memória de sua irmã-esposa? A importância de Arsinoe II pareceu residir na imagem de devoção popular que se criou em torno de sua pessoa, e que acabou favorecendo e prestigiando a dinastia Lagida. A nossa pesquisa se baseia em primeiro lugar na análise das fontes materiais, mas também das fontes textuais tanto do século III a.C. quanto de séculos posteriores. Partimos de uma exposição do fundo histórico em que se baseou e se constituiu a monarquia ptolomaica. Em seguida, refletimos brevemente sobre o papel e o status das rainhas helenísticas e faraônicas na antiguidade. No último capítulo, nos respaldando nos princípios teóricos e metodológicos da arqueologia do culto propostos por Colin Renfrew, fazemos uma longa digressão acerca das razões que levaram Ptolomeu II a estabelecer um culto para Arsinoe II nas esferas grega e egípcia da população. / This research, entitled - Ektheósis Arsinoes: Arsinoe II Philadelphus cult, has as its aim to analyze the reasons, the shape and the diffusion of the religious cult created by Ptolemy II Philadelphus to honor his sister-wife. It is well known that the historical character of Queen Arsinoe II has been controversial since the beginning of studies on Ptolemaic Egypt. From ambitious queen to dedicated wife, there are few documents regarding her life in Egypt in the decade of 280/270 B.C. when she became queen during her brother's reign. The majority of the testimonies are dated to the period after her death. Who was Arsinoe II Philadelphus? Why did Ptolemy II established a cult in memory of his sister-wife? Arsinoe's II importance seems to reside on the image of popular devotion created around her, which ended up favoring and giving prestige to the Lagid dynasty. Our research is based, first of all, on the analysis of material sources, as well as on the written sources both from the 2nd century B.C. and from later centuries. We begin with the discussion of the historical background of the ptolemaic monarchy. Next, we briefly establish some thoughts on the role and the status of the Hellenistic and Pharaonic queens in antiquity. In the last chapter, using Colin Renfrew's theoretical and methodological principles regarding cult archaeology, we make a long digression over the reasons that led Ptolemy II to establish a cult for Arsinoe II both within the Greek and the Egyptian population.

Queen Christina of Sweden (1626-1689)--one of the most celebrated, if controversial, converts of all times--settled in the papal city after her abdication in 1654. Her palace--the Palazzo Riario (today Corsini)--became one of Rome's leading cultural institutions: a site where learned, artistic, and elite culture converged. This study examines Christina's practice of collecting, and argues that her ambition was to create a center for learning and the arts in the Palazzo Riario modeled on the ancient Musaeum of Alexandria. While Christina's importance as a patron of art and learning has long been recognized, this dissertation offers the first comprehensive discussion of Christina's practice of collecting, and the architecture and ambience of her Roman palace. Based on both published and unpublished architectural drawings, inventories, household accounts, and contemporary travel descriptions, this dissertation establishes the contents and the display of Christina's collection, and the architectural plan of the Palazzo Riario. This study examines the intersection between objects and their display, issues of etiquette and decorum, and the social use of architecture in seventeenth-century Rome. It aims to contribute to the history of collecting and early museums, and to the broader field of seventeenth-century culture.

Ropalidia marginata is characterized as a primitively eusocial wasp due to the absence of morphological differentiation between the queen and worker castes. Unlike other primitively eusocial wasps, however, the queen in this species is a docile individual, who does not use aggression to regulate worker reproduction, and does not act as the central pacemaker of her colony. However, if the queen dies or is experimentally removed, one of the workers steps up her aggression immensely within minutes, and if the queen is not replaced, she develops her ovaries, reduces aggression and takes over as the new queen of the colony. We call her the potential queen (PQ). When I started my work on R. marginata, two very intriguing questions were demanding to be answered, which had developed from work done by my immediate seniors in the lab. I decided to pursue both of these for my thesis. My work has been enriched by inputs from several collaborators and colleagues - I couldn’t have done all of it by myself. So, henceforth, I will be using the word “we”, instead of the first person singular to describe the work that has gone into this thesis. Question 1: Is there a designated successor to the queen in R. marginata? My senior Sujata P. Kardile has shown in her thesis, that in R. cyathiformis, a primitively eusocial wasp very closely related to R. marginata, the queen is always succeeded by the next most aggressive individual in the colony, and so the PQ is easily predictable in the presence of the queen. However, in R. marginata, the PQ appears to be an unspecialized individual, who cannot be predicted in the presence of the queen by using age, ovarian profile or behaviour as the yardsticks. However, the PQ becomes evident within minutes after queen removal. The swiftness with which the PQ is established led us to believe that perhaps the successor to the queen in R. marginata is known to the wasps, though we cannot identify her in the presence of the queen. We designed an experiment to check for the presence of such a ”cryptic successor” in R. marginata. Our experiments involved splitting a normal, queen-right nest into two halves separated by a wire mesh partition, so that the wasps could not move across the mesh. Earlier we had used this set-up to demonstrate that a PQ always establishes herself on the queen-less fragment of the nest. So, to test if there is a cryptic successor, we allowed a PQ to establish herself on the queen-less fragment, and then exchanged the queen and the PQ (designated as PQ1) between the two sides. There is a 50% probability that the cryptic successor, if present, would be on the queen-less side in the beginning. Then, upon exchange, she should be able to hold her position on the other side easily. On the other hand, if the cryptic successor is first on the queen-right side, then, upon exchange, she should take over as the PQ (PQ2), and PQ1 should not be able to hold her status. The cryptic successor hypothesis had two predictions: (i) the PQ1 would lose to a PQ2 in about half the cases, and (ii) there would never be a PQ3. We obtained a PQ2 in 5 out of 8 cases, and we never had a PQ3. So we could conclude that there is indeed one individual who is the designated successor to the queen in R. marginata. Since we could not identify her in the presence of the queen, we call her the cryptic successor. The cryptic successor did not receive even a single act of aggression from the PQ1, or from any other individual in the colony. Thus we conclude that she is acceptable to all the wasps in the colony. We next used the more sophisticated and rigorous method of network analysis to check if the PQ could be predicted due to some unique position she might be holding in the social network on her colony. Since this was a first study in a primitively eusocial insect using network tools, we began by characterizing the social networks of queen-right and queen-less colonies of R. marginata, and compared them with the R. cyathiformis networks to see how different the R. marginata society is from a typical primitively eusocial one. The R. marginata social networks based on dominant-subordinate interactions were low in their centrality measure as compared to the R. cyathiformis networks. However, in both the species, the queen-less networks were highly centralized, star-shaped networks with the PQs at the centre. Neither the queens, nor the PQs were key individuals in the queen-right colonies, but it is interesting to note that the removal of an insignificant node, the queen, resulted in a major change in the network architecture, converting the de-centralized queen-right network into a highly centralized one. Such centralized star-shaped networks are unique, and to our knowledge, the first ever described, in any social system. When we removed the queen from the data set (in silico removal), the resulting network was similar in centrality to the queen-right networks. We then did a comparative analysis of the positional importance of the PQs of the two species, and tried to see if we could use this as a tool to predict the PQ in the queen-right network. In R. cyathiformis, the PQs had consistently high ranks (mostly rank 2) in the network based on the degree index, while the PQs in R. marginata had random ranks in the hierarchy. However, since the PQs are known not to have unique ranks in the dominance hierarchies, we repeated the analysis using data on all interactions from the Q-PQ exchange experiments described above. Neither the cryptic successors nor the losers occupied any unique ranks in the all interactions networks. Thus the successors in R. marginata are truly cryptic, even in their social networks. Since R. marginata is known to be more evolved than typical primitively eusocial species, it is likely that the queen’s successor is identified by the wasps through some subtle cue like smell, and so we cannot identify her using the methods that are adequate for the identification of the PQ in a typical primitively eusocial species like R. cyathiformis. Question 2: How does the queen signal her presence and reproductive status to her workers or, how do the workers perceive the presence of their queen? The fact that in spite of her docility, the queen in R. marginata manages to maintain complete reproductive monopoly in her colony, gives rise to the obvious question of how she suppresses worker reproduction. The most attractive hypothesis is that she uses a pheromone like queens of highly eusocial species. My senior A. Sumana had shown that the queen pheromone, if present, is not a volatile substance. She also showed that the queen interacts at a very low rate with her workers, and so they cannot possibly perceive her by means of direct interactions. Since the PQ steps up her aggression within minutes of queen removal, we used her as a proxy to know how soon the queen’s absence is felt in the colony. We built a model to delineate the relationship between the decay time of the pheromone (td), the average age of the queen’s signal present with the PQ (ta), and the average realization time (tr); where tr = td − ta. Using Dijkstra’s algorithm, we showed that the queen could interact faster with the PQ by using relay interactions. Then using experimental data from 50 colonies, we obtained a ta of 102.9 minutes. The td was 340 minutes, and so we obtained a tr of 237.1 minutes; which meant that the PQ should not perceive the queen’s absence within 237 minutes of queen removal, if the queen pheromone is transmitted by a relay mechanism. However, from our experimental data, we had obtained a tr of 30 minutes. So we concluded that physical interactions, both direct and indirect were inadequate for the workers to perceive their queen. As we had ruled out physical interactions, we then wanted to check if it is possible that the queen applies her pheromone to the nest material, from where it is perceived by the workers when they walk or sit on the nest, or antennate the nest surface. The “rub abdomen behaviour (RA)” has been observed to be quite typical of R. marginata queens, and is not very common in the workers of the species. RA involves rubbing the ventral side of the tip of the abdomen or dragging it on the nest surface while walking. We thought that the queen might be using this behaviour to apply her pheromone on the nest material. So we characterized this behaviour using focal behaviour sampling, and found that the queen rubs her abdomen on the nest once in every 23 minutes. Since the observed tr is 30 minutes, it is quite likely that the queen uses the rub abdomen behaviour to apply her pheromone on the nest. The next step was to check for the source of the queen pheromone. We looked for glands that open near the base of the sting, and the Dufour’s gland was a good choice, as it is known to be involved in the recognition of egg-laying workers in the honeybees. We performed a bioassay in the blind using the crude extract of the Dufour’s gland (prepared in Ringer’s solution) from the queen. The Dufour’s gland extract of a randomly chosen worker and the solvent were used as controls. We found that the PQ responds to the queen’s Dufour’s gland extract by lowering her aggression to 65% of what she was showing on queen removal and before the application of the extract. However, the PQ did not change her behaviour significantly when the worker’s extract or Ringer’s solution was applied. The PQ’s reduction of aggression on application of the queen’s extract mimicked the reaction of PQ’s when the queen is re-introduced on the nest some time after removal. So we hypothesize that the Dufour’s gland is the source of the queen pheromone (signal) in R. marginata. This thesis has opened up newer questions pertaining to the power of the queen and the intricacies of the succession to power in R. marginata. For example, we need to pursue chemical analyses of the Dufour’s gland extract of R. marginata to have conclusive proof of it’s being the source of the queen pheromone. But that is perhaps suitable topic for my juniors in the lab, who can continue the tradition of beginning with questions opened up by their seniors!

This dissertation addresses two questions fundamental to Russian nineteenth-century intellectual history: 1) Why does literature about paranoid psychosis figure so centrally in the nineteenth-century canon? and 2) How did the absence of an epistemological tradition of reflexive self-consciousness influence the development of Russian ideas of subjectivity? I propose that the presence of paranoia in Russian fiction extends beyond the medical or psychoanalytic aspects of character traits or themes. I argue that literary representations of paranoia perform fundamental philosophical gestures and function as "epistemological speech acts." Russian narratives of paranoia (e.g., Pushkin, Gogol, Dostoevsky, Chekhov, Garshin, Sologub) constitute a means of exploring the operations of a self-reflexive consciousness, familiar in the West through the Cartesian Cogito. In other words, the theme of paranoia in nineteenth-century Russian fiction actively responds to the regnant philosophical discourse and functions as a praxis for the exploration of philosophical questions. However, this is done in an alternative discourse to the propositional language generally favored in philosophical texts; as a result, the philosophical function of the fictions of paranoia has gone unrecognized, and the genre has been "exiled" from philosophical discourse. I argue that Russian texts of paranoid psychosis should be reconceived as venues for the play of the transcendental ego outside social or communal axes. Paranoia emerges as the Jakobsonian “dominant” within these texts, in that it is paranoia that engages with other narrative components and transforms them. Further, as prose fiction, these texts had the discursive and social capacity to resonate and divagate in ways impossible to philosophical texts. Ultimately, these narratives of paranoia are meta-epistemologies that interrogate their own discursive function and status. They raise critical questions not only about the ways in which we represent truth but about the ontological status of truth itself. / Slavic Languages and Literatures