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The Global ETD Search service is a free service for researchers
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Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Showing 441 to 449 of 449 (0.027 seconds)Spelling suggestions: "subject:"seen""
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Engineering antibodies to study and improve immunomagnetic isolation of tumour cellsJain, Jayati January 2013 (has links)
Cell separation based on antibody-targeted magnetic beads has been widely used in a number of applications in immunology, microbiology, oncology and more recently, in the isolation of circulating tumour cells (CTCs) in cancer patients. Although other cell separation techniques such as size based cell filtration and Fluorescence Activated Cell Sorting have also been in popular use, immunomagnetic cell isolation possesses the advantages of high throughput, good specificity and reduced cell stress. However, certain fundamental features of the cell-bead interface are still unknown. In this study, some of the key features of the cell-bead synapse were investigated in an effort to improve the efficiency of immunomagnetic cell isolation and reduce its dependence on high expressing cell surface markers. A clinically relevant antibody fragment (Fab) against tyrosine kinase receptor HER2 was applied to study the immunomagnetic isolation of HER2 expressing cancer cells. First, the minimum number of target proteins required on a cell for it to be isolated was determined. Second, the importance of the primary antibody affinity was investigated, using a series of Fab mutants with known kinetics and it was shown that despite starting with sub-nanomolar affinity, improving Fab affinity increased cell isolation. Third, the influence of the connection between the primary antibody and the bead was studied by comparing Fab bridged to the magnetic bead via a secondary antibody, Protein L or streptavidin; the high affinity biotin-streptavidin linkage increased isolation sensitivity by an order of magnitude. Fourth, the effect of manipulating cytoskeletal polymerization and cell membrane fluidity using small molecules was tested; cholesterol depletion decreased isolation and cholesterol loading increased cell isolation. The insights from these observations were then applied to isolate a panel of cell lines expressing a wide range of surface HER2. While the standard approach isolated less than 10% of low HER2 expressing cancer cells from spiked rabbit and human blood, our enhanced approach with the optimized cholesterol level, antibody affinity and antibody-bead linkage could specifically isolate more than 80% of such cells. The final part of this work focussed on developing an antibody clamp that could physically restrict the antigen within its binding site on the Fab and prevent antigen dissociation, using the HER2-Fab complex and the anti-myc peptide antibody 9E10. Work from this thesis provides useful insights into the molecular and cellular parameters guiding immunomagnetic cell isolation and can be used to extend the range of target receptors and biomarkers for tumour cell isolation and other types of cell separation, thereby enhancing the power and capacity of this approach.
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Microbial sulfate reduction in the tissue of the cold-water sponge Geodia barretti (Tetractinellida, Demospongiea) / Mikrobielle Sulfatreduktion im Gewebe des Kaltwasserschwammes Geodia barretti (Tetractinellida, Demospongiae)Hoffmann, Friederike 06 May 2003 (has links)
No description available.
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[en] A PREFERENTIAL OPTION FOR THE BODY: THE CONTRIBUTION OF RUBEM ALVES TO THE LIBERATION THEOLOGY / [es] OPCIÓN PREFERENCIAL POR EL CUERPO: EN CONTRIBUICIÓN DE RUBEM ALVES A TEOLOGÍA DE LA LIBERACIÓN / [pt] OPÇÃO PREFERENCIAL PELO CORPO: A CONTRIBUIÇÃO DE RUBEM ALVES À TEOLOGIA DA LIBERTAÇÃORAINERSON ISRAEL ESTEVAM DE LUIZ 21 December 2017 (has links)
[pt] Esta tese propõe uma reflexão, à luz das Escrituras Sagradas e da fé cristã, sobre a inovadora proposta para a Teologia da Libertação de Rubem Alves. Inicialmente, discute-se a Teologia Latino-Americana da Libertação (TdL), tentando investigar seus influxos das Ciências do Social (CdS), as quais forneceram o ferramental necessário para abordar o concreto-pensado sobre o qual a TdL teologizou. Pretende-se mostrar como Rubem Alves abriu mão de uma gramática marxista (realidade penúltima) e assumiu uma linguagem mais abrangente, humanista, interdisciplinar e holística sobre a liberdade e essência humana mediante uma interpretação inovadora de não apenas textos bíblicos e evangélicos, mas também de muitos poetas, teólogos, filósofos e uma gama ampla de outros pensadores sociais. A partir do diálogo interdisciplinar e suas próprias inquietudes e vivências dolorosas, Rubem Alves centralizou o corpo como a prioridade axiológica de seu que-fazer teológico da Libertação. Finalmente, propõe-se uma Teologia lúdica da Libertação mais integral, poética e transcendente a partir do método pastoral tripartido ver-julgar-agir que, além de levar em conta a multidimensionalidade e complexidade da essência humana que o corpo expressa, assume uma linguagem mais dionisíaca e polifônica na busca pela libertação humana não só da servidão econômica, mas de todas as formas de repressão. / [en] This dissertation posits a scriptural and Christian faith-based reflection on Brazilian theologian Rubem Alves innovative Liberation Theology proposal. Initially, we examine Latin American Liberation Theology (abbreviated TdL herein) and attempt to trace how it was influenced by the Social Sciences (abbreviated CdS herein) which supplied Liberation Theology with the necessary tools for theologizing the thought concrete. We aim to show how Rubem Alves cast off a Marxist grammar (penultimate reality) in lieu of a broader, more humanistic, interdisciplinary and holistic discourse regarding human freedom and personhood by innovatively interpreting not only Biblical and Gospel texts but also those of poets, theologians, philosophers and a wide range of other social thinkers. From his interdisciplinary dialogs, his personal soul-searching and painful life experiences, Alves centered the body as the axiological priority of his Liberation Theology undertaking. Finally, we propose a more holistic, poetic, playful and transcendental Liberation Theology based on the threefold see-judge-act pastoral process that, beside focusing on the multidimensional and complex human personhood the body expresses, incorporates a more Dionysian and polyphonic discourse in its search for not only human economic freedom, but freedom from all forms of repression. / [es] En esta tesis, se propone una reflexión a la luz de las Sagradas Escrituras y de la fe cristiana sobre la innovadora propuesta de la Teología de la Liberación del teólogo brasileño Rubem Alves. Se analiza primero la Teología de la Liberación en América Latina (aquí abreviada como TdL), tratando de investigar la influencia que sufrió de las Ciencias Sociales (aquí abreviadas como CdS), las cuales le prestaron las herramientas necesarias para teologizar sobre lo concreto-pensado. Se pretende mostrar cómo Rubem Alves abandonó una gramática marxista (penúltima realidad) y asumió un lenguaje humanista, interdisciplinario e integral más amplio sobre la libertad humana mediante una interpretación innovadora, no sólo de los textos bíblicos y evangélicos, sino de muchos poetas, teólogos, filósofos y una amplia gama
de otros pensadores. Desde el diálogo interdisciplinario y sus propias inquietudes y experiencias dolorosas, Alves centró al cuerpo como la prioridad axiológica de su Teología de la Liberación. Por último, se propone una Teología de la Liberación más integral, poética, trascendente y lúdica a partir del método pastoral tripartito ver-juzgar-actuar que, además de tomar en cuenta la multidimensionalidad y complejidad de la esencia humana que el cuerpo expresa, se vale de un lenguaje más dionisíaco y polifónica en la búsqueda de la liberación humana no sólo de la servidumbre económica, sino de todas las formas de represión.
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Chemical and biological studies on human oxygenasesThinnes, Cyrille Christophe January 2014 (has links)
As depicted in Chapter I, 2-oxoglutarate- (2OG) dependent oxygenases are ubiquitous in living systems and display a wide range of cellular functions, spanning metabolism, transcription, and translation. Although functionally diverse, the 2OG oxygenases share a high degree of structural similarities between their catalytic sites. From a medicinal chemistry point of view, the combination of biological diversity and structural similarity presents a rather challenging task for the development of selective small molecules for functional studies in vivo. The non-selective metal chelator 8-hydroxyquinoline (8HQ) was used as a template for the generation of tool compound <b>I</b> for the KDM4 subfamily of histone demethylases via application of the Betti reaction. Structural analogue <b>II</b> was used as the corresponding negative control (Figure A). These compounds were characterised in vitro against a range of 2OG oxygenases and subsequently used for studies in cells. <b>I</b> displays selectivity for KDM4 and increases the level of the H3K9me3 histone mark in cells. It has an effect on the post-translational modification pattern of histone H3, but not other histones, and reduces the viability of lung cancer cells, but not normal lung cells, derived from the same patient. <b>I</b> also stabilises hypoxia-inducable factor HIF in cells via a mechanism which seems to be independent from prolyl hydroxylase inhibition. This work is described in Chapters II and III. The chemical biology research in epigenetics is complemented by qualitative analysis conducted in the social sciences at Said Business School. With a global view on how innovation occurs and may actively be fostered, Chapter IV focuses on the potential of epigenetics in drug discovery and how this process may actively be promoted within the framework of open innovation. Areas of focus include considerations of incremental and disruptive technology; how to claim, demarcate, and control the market; how knowledge brokering occurs; and insights about process, management, organisation, and culture of open innovation. In contrast to the open-skies approach adopted for the development of a tool compound in Chapters II and III, a focused-library approach was taken for the generation of a tool compound for the OGFOD1 ribosomal prolyl hydroxylase. The development of a suitable in vitro activity assay for OGFOD1 in Chapter V enabled the development of lead compound <b>III</b> in Chapter VI. <b>III</b> is selective for OGFOD1 against the structurally closely related prolyl hydroxylase PHD2.
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Development Finance Institutions’ Effect on The Fund Manager’s Investment Decisions : Balancing Financial Performance Goals and Development Impact ObjectivesAdolfssson, Alexander, Åström, Marie January 2016 (has links)
Development Finance Institutions (DFIs) have played a crucial role in moving socially responsibility considerations up on the private equity industry’s agenda. DFIs add a development impact criterion to traditional financial performance goals in the investment industry and play a catalytic role by mobilizing other investors. The gap in research regarding DFIs implications and significance in the investment community from a SRI perspective is evident. The development impact objective introduced by the DFIs is examined to understand its effects on fund managers’ decision-making and if it exists a trade-off between this objective and financial performance. An understanding of how DFIs control fund managers to act in accordance to their objective as well as how they determine compensation schemes to incentivize them to pursue high return on investments, is discussed in relation to the agency theory. Furthermore, stakeholder/shareholder consideration is examined in relation to the subject. The aim of this study is to examine how the behavior of fund managers is affected by the involvement of a DFI investor and try to add to the understanding of their significance as institutional investors in developing markets. Previous studies have been more focused on determining the financial performance of socially responsible investments by using very similar quantitative data collection methods. This thesis undertakes an in-depth approach with the purpose to understand the fund manager’s drives as well as how a DFI involvement affects the behavior and decision-making process. This thesis undertook a qualitative research strategy and semi-structured interviews were used as the tool to understand the fund managers’ personals beliefs and perceptions of how the relationship with DFIs affect them. The selection criteria for the fund managers was that they needed to work in a fund in which a DFIs has invested. We also included DFI investors in order to understand their point of view. The interview was recorded, transcribed and later divided into themes in accordance with the thematic approach, following six steps. Our findings show that Development Finance Institutions plays an important role in emerging markets and affect fund manager behavior to a certain extent. They did not perceive a trade-off between financial performance goals and development impact objectives. We conclude that DFIs increase fund manager focus on ESG/SEE elements in the investment process. DFIs requirements and reporting obligations is used as a tool to ensure that the fund manager act in accordance to DFI objective. The fund managers were neither willing to sacrifice commercial return in favor of development impact. Lastly, the interest among the DFIs and commercial investors is fairly similar, hence reducing the conflict of interest between investors.
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Geochemie Porifera-reicher Mud Mounds und Mikrobialithe des Mittel- und Oberdevons (Westaustralien, Nordfrankreich) / Geochemistry of Porifera-rich mud mounds and microbialites of the Middle and Upper Devonian (Western Australia, Northern France)Hühne, Cathrin 07 November 2005 (has links)
No description available.
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Opera i Stockholm, GalärvarvetAndersson, Joakim January 2011 (has links)
Suggestion for a new operahouse in stockholm, förslag på nytt operahus i stockholm. Stockholmsoperan.
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UTILIZATION OF WIND POWER IN RWANDA : Design and Production OptionEric, MANIRAGUHA January 2013 (has links)
This Master Thesis is the research done in the country of Rwanda. The project leads to study the climate of this country in order to establish whether this climate could be used to produce energy from air and to implement the first wind turbine for serving the nation. After an introduction about the historical background of wind power, the thesis work deals with assessment of wind energy potential of Rwanda in focusing of the most suitable place for wind power plants. The best location with annual mean wind speed, the rate of use of turbine with hub height for an annual production per year, the mean wind speeds for 6 sites of Rwanda based on ECMWF for climatic data for one year at relief of altitude of 100m and coordinates are reported too. The result of energy produced and calculations were done based on power hitting wind turbine generator in order to calculate Kinetic energy and power available at the best location to the measurement over the period of 12 months, that could be hoped for long term. With help of logarithmic law, where wind speed usually increases with increasing in elevation and the desired wind speeds at all 6 sites were used. The annual energy production was taken into account at the best site with desired wind speed at the initial cost of turbine as well as the cost of energy (COE).However, with comparison of the tariff of EWSA, the price of Wind designed in this Research per kWh is cheaper and suitable for people of Rwanda. / <p><em>Rwanda has considerable opportunities development energy from hydro sources, methane gas, solar and peat deposits. Most of these energy sources have not been fully exploited, such as solar, wind and geothermal. As such wood is still being the major source of energy for 94 per cent of the population and imported petroleum products consume more than 40 per cent of foreign exchange. Energy is a key component of the Rwandan economy. It is thus recognized that the current inadequate and expensive energy supply constitutes a limiting factor to sustainable development. Rwanda’s Vision 2020 emphasizes the need for economic growth, private investment and economic transformation supported by a reliable and affordable energy supply as a key factor for the development process. To achieve this transformation, the country will need to increase energy production and diversify into alternative energy sources. Rwandan nations don’t have small-scale solar, wind, and geothermal devices in operation providing energy to urban and rural areas. These types of energy production are especially useful in remote locations because of the excessive cost of transporting electricity from large-scale power plants. The application of renewable energy technology has the potential to alleviate many of the problems that face the people of Rwanda every day, especially if done so in a sustainable manner that prioritizes human rights.</em></p>
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Slovinské národní divadlo v Lublani / Slovene National Theatre in LjubljanaHýl, Petr January 2009 (has links)
SLOVENE NATIONAL THEATRE IN LJUBLJANA Author Report Of The Diploma Work Author: Bc. Petr Hýl Supervisor: doc. ing. arch. Zdeněk Makovský
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