Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2016-03-31T20:17:42Z No. of bitstreams: 2 Dissertação - Mariana Vaz Bisneta - 2015.pdf: 6629201 bytes, checksum: b7081f288e6426640f564925ba69b856 (MD5) license_rdf: 19874 bytes, checksum: 38cb62ef53e6f513db2fb7e337df6485 (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-04-04T14:26:36Z (GMT) No. of bitstreams: 2 Dissertação - Mariana Vaz Bisneta - 2015.pdf: 6629201 bytes, checksum: b7081f288e6426640f564925ba69b856 (MD5) license_rdf: 19874 bytes, checksum: 38cb62ef53e6f513db2fb7e337df6485 (MD5) / Made available in DSpace on 2016-04-04T14:26:36Z (GMT). No. of bitstreams: 2 Dissertação - Mariana Vaz Bisneta - 2015.pdf: 6629201 bytes, checksum: b7081f288e6426640f564925ba69b856 (MD5) license_rdf: 19874 bytes, checksum: 38cb62ef53e6f513db2fb7e337df6485 (MD5) Previous issue date: 2015-02-20 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / In Brazil determinate stem termination soybean plants with long juvenile period are traditionally grown. In the last five years, indeterminate and semideterminate soybean plants, especially early maturity varieties in early sowing dates, were adopted in different latitudes, aiming to cultivate a second crop. When grown on different densities, soybean plants have compensation mechanisms on stem height, number of branches and pods per plant. The objectives of this study were: i) evaluate the effect of sowing date and plant density on soybean morphoagronomic traits of soybean cultivars with different stem termination; ii) correlate grain yield with morphoagronomic traits in each type of growth and sowing date, and yield components with each other in each type of growth and density; iii) perform discrimination among types of growth in soybean cultivars, identifying sowing conditions that cause changes in the response of morphological traits with ambiguity in the phenotypic expression of growth types. Three experiments were: early October (10.02.13), mid November (11.18.13) and early January (08.01.14) in the experimental area of Embrapa Arroz e Feijão in Santo Antonio de Goiás, Brazil (16°29’S, 49°17’W), at 2013/2014 season. Trials were performed in a randomized complete block design with three replications. Treatments included two factors, stem termination and plant population density. Four cultivars were used for each stem termination; determined, semideterminate and indeterminate. The plant densities corresponded to 50%, 100% and 150% of commercial recommendation to each variety. The traits assessed were number of days to flowering, number of days to maturity, plant height at flowering, height of first pod, plant height at maturity, growth percentage after flowering, number of nodes on main stem, number of branches on main stem, number of pods per node, number of pods per plant, number of grains per pod, number of grains per plant, one hundred grain weight and yield. For most assessed traits, soybean plants of each type of growth respond differently to changes on sowing date and plant density. Only in November sowing, the determinate type of growth showed lower yield comparing to the others types. The increase in plant density causes higher plants on flowering and maturity, less number of nodes and branches in the main stem, and lower average on yield components. Regardless of the type of growth, in the first sowing date yield showed greater correlation, positive, with number of seeds per pod; however, in other sowing dates this correlation occurred with one hundred grain weight. Plant density has more influence on the associations among yield components in the determinate and indeterminate growth types, than in the semideterminate type. The greatest variation in morphoagronomic traits was observed between determinate stem termination and the other types of growth. Most change on classification of type of growth (ambiguity) occurs in late sowing and high plant density. The percentages of growth after flowering and main stem node and branches numbers are the most important traits on type of growth discrimination. / No Brasil tradicionalmente são cultivadas plantas de soja com tipo de crescimento determinado e portadoras de período juvenil longo. Nos últimos cinco anos, plantas de soja com tipo de crescimento indeterminado e semideterminado, sobretudo de ciclo precoce, nas várias faixas de latitude, passaram a ser adotadas no início da época de semeadura, visando o cultivo da segunda safra (safrinha). Quando cultivada em diferentes densidades populacionais, as plantas de soja apresentam mecanismos de compensação, por exemplo, na altura, no número de ramificações e de vagens por planta. Assim, os objetivos deste trabalho foram: i) avaliar o efeito da época e densidade de semeadura em caracteres morfoagronômicos de plantas de soja com diferentes tipos de crescimento; ii) correlacionar a produtividade de grãos com caracteres morfoagronômicos, em cada tipo de crescimento e época de semeadura, assim como os componentes de produção entre si, em cada tipo de crescimento e densidade de plantas, buscando-se identificar mudanças nas associações, decorrentes do efeito da densidade de plantas; iii) discriminar os tipos de crescimento em cultivares de soja, identificando condições de semeadura que provoquem mudanças na resposta de caracteres morfoagronômicos, implicando em ambiguidade na expressão fenotípica dos tipos de crescimento. Foram instalados três experimentos em diferentes épocas de semeadura: início de outubro (02/10/13), meados de novembro (18/11/13) e início de janeiro (08/01/14), em área experimental da Embrapa Arroz e Feijão, em Santo Antônio de Goiás (16°29’S, 49°17’W), na safra 2013/2014. Os ensaios foram realizados em delineamento de blocos completos casualizados, com três repetições. Os tratamentos incluíram dois fatores, tipos de crescimento e densidades de semeadura. Foram utilizadas quatro cultivares para cada tipo de crescimento; determinado, semideterminado e indeterminado. As densidades de plantas corresponderam a 50%, 100% e 150% da população recomendada comercialmente para cada cultivar. Foram avaliados 14 caracteres; número de dias para florescimento, número de dias para maturação, altura da planta na floração, altura de inserção da primeira vagem, altura de planta na maturação, porcentagem de crescimento após a floração, número de nós na haste principal, número de ramificações na haste principal, número de vagens por nó, número de vagens por planta, número de grãos por vagem, número de grãos por planta, massa de cem grãos e produtividade. Para a maioria dos caracteres estudados, as plantas de cada tipo de crescimento responderam diferentemente às variações de época e densidade de semeadura. Apenas na semeadura de novembro o tipo de crescimento determinado resultou em menor produtividade que os outros tipos. O aumento da densidade de plantas provocou maior altura de plantas, tanto na floração como na maturação; assim como menor número de nós e de ramificações na haste principal e menor média dos componentes de produção. Independentemente do tipo de crescimento, na primeira época de semeadura a produtividade mostrou-se correlacionada positivamente e em maior magnitude com o número de grãos por vagem; já nas outras épocas, a correlação foi maior com a massa de cem grãos. A densidade influencia mais a associação entre os componentes de produção nos tipos de crescimento determinado e indeterminado do que no tipo semideterminado. A maior variação nos caracteres morfoagrônomicos ocorreu entre o tipo determinado e os outros tipos. A plasticidade de tipos de crescimento (ambiguidade) ocorre, principalmente, na semeadura tardia e sob alta densidade de plantas. A porcentagem de crescimento após a floração, número de ramificações e de nós na haste principal são as variáveis mais importantes na discriminação dos tipos de crescimento.

A presente pesquisa está inserida na questão da infraestrutura logística para o transporte de carga a granel, especificamente a soja, a partir das principais mesorregiões produtoras dos dois maiores produtores mundiais, Brasil e Estados Unidos, até os principais portos exportadores. Este trabalho tem por objetivo analisar a eficiência das rotas e corredores nacionais de transporte de soja, levando em consideração os três pilares da sustentabilidade (econômico, social e ambiental). O método parte de uma revisão de literatura sobre o tema, seguido de uma explanação sobre eficiência, por meio da Análise Envoltória de Dados (DEA), e uma contextualização do cenário de escoamento em ambos os países. Foram determinadas 72 rotas brasileiras e 30 norte-americanas com diversas composições de modais. Os dados foram analisados por correlação e análise de componentes principais (PCA) e teste de Wilcoxon. Em seguida, as variáveis foram inseridas num modelo DEA de Slack-Based Measure (SBM), que minimiza os inputs (variáveis de entrada) e maximiza outputs (variáveis de saída) simultaneamente. Foi aplicado, como técnica quantitativa de desempate, o índice composto. Os resultados mostraram que as rotas norte-americanas foram as mais eficientes. Além disso, combinações de curtos trajetos de caminhão e longas rotas de modais hidroviários ou ferroviários estavam entre as eficientes. Ou seja, os resultados se mostraram coerentes com a literatura e a expectativa de especialistas. Em resumo, o uso da DEA pode notadamente fortalecer a análise de rotas e corredores domésticos nacionais usados para o escoamento produtivo da soja a granel e pode direcionar políticas de investimento públicas e privadas. / This research is focused on the logistics infrastructure issue used for bulk transportation, specifically soybeans, from the main producing municipalities of the world biggest producers, Brazil and USA, to the key exporting ports. This dissertation aims to analyze the efficiency of routes and corridors, taking into account the three pillars of sustainability (economic, social and environmental). The method starts with a literature review of corridor benchmarking, efficiency measurement through data envelopment analysis and contextualization of transportation scenarios in both countries. It was identified 72 routes in Brazil and 30 in the US, considering several combinations of transportation mode (road, inland waterway and rail). Data were analyzed through correlation, principal component analysis (PCA), and Wilcoxon Test. Subsequently, variables were inserted in Slack-Based Measure (SBM) DEA model. This model simultaneously minimizes inputs and maximizes outputs. It was applied the quantitative tiebreaking method of the compound index. The results showed that the American routes among the most efficient ones. In addition, the combinations of short trips of trucks and long trips of barges or trains were also among the most efficient. In other words, the results were coherent with literature and expectation of specialists. In summary, it is clear that the use of DEA can noticeably improve the strength of the analysis of national domestic routes and corridors used in the productive chain of bulk soybeans.

O conhecimento da herança dos caracteres de interesse é essencial para programas de melhoramento genético. Entretanto, a herança é altamente influenciada pelo ambiente e pela constituição genotípica da população e, assim, o acúmulo de informações é de grande importância para um melhor conhecimento das heranças dos caracteres. A maior parte dos estudos de herança de caracteres quantitativos é baseada em genótipos que não são mais usados em programas de melhoramento. Este trabalho teve como objetivo o estudo da herança da produção de grãos (PG) e dos componentes de produção em soja, isto é, número de vagens por planta (VP), número de sementes por planta (SP) e número de sementes por vagem (SV), em uma população derivada do cruzamento entre os cultivares Embrapa 60 e MG/BR 46, de alta divergência genética. Os genitores e as gerações F1, F2 e os dois retrocruzamentos foram avaliados experimentalmente no ano agrícola de 2007/8 para os quatro caracteres no Departamento de Genética da ESALQ, em Piracicaba, SP. Os dados experimentais foram submetidos às análises genéticas segundo o modelo aditivodominante, proposto por Mather e Jinks. As estimativas da heterose foram positivas para três dos caracteres (PG, VP e SP), e em torno de 50%, em relação à média dos genitores, enquanto que para SV a heterose foi nula. A herdabilidade entre plantas foi mediana para os componentes da produção (entre 33% e 42%) e baixa para PG (16%). As estimativas das variâncias aditivas foram sempre superiores às da variância dominante, de forma que os graus médios de dominância para todos os caracteres foram em torno de 1,0 (0,96 a 1,21), indicando a ocorrência de dominância completa para os caracteres avaliados. / The knowledge of inheritance of traits is very important for breeding purposes. However, the inheritance is highly influenced by the environment and the genotypic composition of the population and thus, the accumulative information of estimates is very important for a better knowledge of the inheritance of quantitative traits. Most of the studies of inheritance of quantitative traits in soybeans are based on populations which nowadays have no more interest in soybean breeding programs. The objective of this study was to investigate the inheritance of grain yield (PG) and yield components in soybeans, i.e., number of pods per plant (VP), number of seeds per plant (SP), and number of seeds per pod (SV), in a population derived from two genetically divergent soybean cultivars: Embrapa 60 and MG/BR 46. The parents, F1, F2 and two backcross generations were evaluated in the 2007/8 growing season for the four traits, at the Department of Genetics (ESALQ), in Piracicaba, SP. Experimental data were submitted to genetic analyses according to the additive-dominant model proposed by Mather and Jinks. Heterosis estimates were positive for three out of four traits (PG, VP, SP), with magnitudes around 50%, based on the parent means, while for SV heterosis was null. The heritability estimates on a plant basis were medium for yield components (between 33% and 42%) and low for PG (16%). Additive variance estimates were always higher than the dominance estimates, and the degree of dominance (gmd) for all the traits were around 1.0 (0.96 to 1.21), indicating the occurrence of complete dominance for the traits assessed.

L’Amazonie est soumise depuis plusieurs décennies à un changement rapide d’occupation du sol du fait de la déforestation, l’installation de systèmes de production agricole, notamment l’élevage bovin et plus récemment le soja. L’un des défis majeurs est de mieux comprendre la dynamique spatiale de ces processus et ses déterminants. Avec la réduction de la déforestation et l’évolution agraire sur les fronts pionniers, les ressources et contraintes naturelles semblent gagner en importance dans les choix opérés par les agriculteurs. L'objectif de la présente recherche est d'évaluer le rôle des facteurs biophysiques dans la dynamique de l’occupation du sol depuis 2000 et d’anticiper sur des futurs possibles dans un territoire amazonien d’ancien front pionnier, aujourd’hui consolidé, la municipalité de Paragominas. Nous avons mis en œuvre un modèle spatialement explicite, afin d’identifier la distribution spatiale des processus de changement en relation avec différents facteurs. Des entretiens réalisés avec des producteurs explicitent les logiques sous-jacentes. Les résultats montrent qu'il y a différentes logiques d'usage des ressources biophysiques, qui varient selon les types de production, la taille des exploitations, la distance aux routes et villages, et la distribution spatiale des ressources biophysiques dans l'exploitation. Elles évoluent au fil du temps, en fonction des productions et des avancées technologiques, et sont organisées dans l'espace. La mise en évidence de ces relations spatiales constitue une avancée significative pour représenter l'évolution de l’ usage des sols et constituer un appui dans la définition de politiques de développement territorial. / The Amazon has been subject to a rapid change in land use due to deforestation for several decades and more recently to the expansion of annual crops like soybeans. One of the major challenges is to understand better the spatial dynamics of these processes and its determinants. With the reduction of deforestation and the consolidation of pioneer fronts, natural resources and constraints seem to be gaining in importance in farmers' choices. The objective of this research was to assess the role of biophysical factors in land use dynamics since 2000 and to anticipate possible futures in a consolidated Amazonian pioneer frontier, the municipality of Paragominas. We implemented a spatially explicit model to identify the spatial distribution of change processes in relation to different factors. Interviews with farmers explain the underlying strategy. The results show different strategies for the use of biophysical resources according to the types of production, the size of the farms, the distance to roads and villages and the spatial distribution of biophysical resources in the farm. The strategies evolve over time, in relation to production and technological advances, and are organized in space. A better understanding of the importance of biophysical resources on the evolution of pioneer fronts through this type of method could provide support for land-use policies.

No description available.

Ce mémoire propose une hypothèse alternative pour expliquer les facteurs à l’origine du boom du soja brésilien. En utilisant une approche combinant la théorie de l’organisation et l’économie politique institutionnelle, ce mémoire propose de comparer les modèles d’organisation de la chaîne de valeur soja des États du Mato Grosso et du Paraná. Dans un premier temps, cette recherche est guidée par un souci de documenter les rouages propres à chaque mode d’organisation et de catégoriser ces derniers comme des systèmes à part entière et cohérents. Ainsi, le mode de coordination du Mato Grosso (intégration de la chaîne de valeur) peut être qualifié de modèle hybride se rapprochant davantage du pôle hiérarchie tandis que le mode d’organisation du Paraná (coopérative agroindustrielle) peut être qualifié de mode hybride gravitant davantage vers le pôle marché. La nature des risques est identifiée comme principal déterminant du degré de centralisation de la gouvernance au sein de ces modèles. L’argument soutient que chaque mode de coordination est performant car il est adapté aux conditions locales de production et permet de mitiger les risques propres à chaque région de façon à encourager les échanges économiques. Dans un deuxième temps, cette recherche serait incomplète sans une analyse des facteurs politiques et historiques ayant influencé la trajectoire de développement de chaque région. Ainsi, chaque étude de cas présente un récit détaillé de l’évolution des politiques agraires et des institutions depuis les années 1960, soit le moment où la culture commerciale du soja a commencé à gagner en importance au Brésil. Analyser le changement institutionnel depuis 1960 est primordial pour comprendre comment se sont consolidées les règles du jeu qui sous-tendent aujourd’hui chaque mode de coordination. / This dissertation proposes an alternative argument for explaining the Brazilian soybean boom. Drawing on the theory of organization and on institutional political economy, this research compares the organization of the value chain in the states of Mato Grosso and Paraná. Firstly, this research attempts to detail the internal workings of each mode of organization in order to conceptualize the latter as integral and coherent systems. As a result, Mato Grosso’s coordination mode (supply-chain integration) may be described as a hybrid organizational mode bearing resemblances with hierarchical organization whereas Paraná’s hybrid mode (agro industrial cooperative) tilts towards market organization. We identify the nature of risks as the primary determinant of the degree of governance centralization within each model. As a result, it is argued that each model is coherent and efficient because it serves the purpose of mitigating risks in a way that is responsive and adapted to local production imperatives. Such consideration is of upmost importance given that risk mitigation may encourage transactions and hence, induce economic growth. Secondly, such a research endeavour would be incomplete without a detailed analysis of the political and historical factors that influenced the development trajectory of each region. In order to take those variables into account, the case studies trace the history of the agrarian policies and institutions that shaped the economic landscape of each region starting from the 1960s, this is, when the production of soybeans started gaining importance in Brazil. Analyzing institutional change, it is argued, is critical for understanding the rules of the game that underpin the actual modes of coordination in each region.

Os objetivos deste experimento foram avaliar os efeitos da substituição do milho e do feno de coastcross pela casca de soja (CS) no desempenho e comportamento ingestivo de cabras em lactação e na digestibilidade dos nutrientes das rações e parâmetros ruminais de borregos Santa Inês. Foram realizados quatro experimentos. Experimento 1: Oito cabras foram alojadas em baias tipo tie stall e distribuídas em um delineamento de quadrado latino duplo 4x4, com duração de cada período de 16 dias, sendo 12 dias para adaptação às rações experimentais e quatro dias para colheita das amostras. Os animais foram alimentados com dietas compostas por 40% de volumoso e 60% de concentrado. A CS substituiu o milho em 0, 20, 40 e 60%, da matéria seca (MS). Não houve efeito (P>0,05) no consumo de MS, produção de leite e no comportamento ingestivo, contudo, o teor de gordura no leite aumentou (P<0,05) com a inclusão da CS na ração. Experimento 2: Quatro borregos foram alojados em gaiolas metálicas para ensaio de metabolismo e distribuídos em delineamento experimental quadrado latino 4x4. Os períodos foram de 14 dias, sendo 10 dias para a adaptação dos animais às rações experimentais e 4 dias para colheita das amostras. Os tratamentos e as dietas foram semelhantes ao Experimento 1. Não houve efeito (P>0,05) da substituição do milho pela CS para o consumo e digestibilidade da MS. No entanto, ocorreu diminuição (P<0,05) da concentração de propionato no rúmen e aumento (P<0,05) da digestibilidade da fibra em detergente neutro (FDN), do pH ruminal e da concentração de acetato. Experimento 3: Trinta e seis cabras foram alojadas em baias tipo tie stall e distribuídas em delineamento experimental de blocos completos casualizados pelo período de oito semanas. As cabras foram alimentadas com dietas compostas por 50% de volumoso e 50% de concentrado e com o mesmo teor de FDN. A CS substituiu o feno de coastcross em 0, 33, 67 ou 100% da MS. O consumo de MS e FDN apresentaram efeito quadrático (P<0,05). A produção de leite e a variação do peso corporal não foram alteradas (P>0,05), porém a concentração de gordura aumentou (P<0,05) e os tempos gastos com ruminação e mastigação decresceram (P<0,05) com a inclusão da CS na dieta. Experimento 4: Dezesseis borregos foram alojados em gaiolas metálicas para ensaio de metabolismo e distribuídos em delineamento experimental blocos completos casualizados. O experimento teve duração de 14 dias, sendo 10 dias para a adaptação dos animais às rações experimentais e 4 dias para colheita das amostras. Os tratamentos e as dietas foram semelhantes ao Experimento 3. O consumo de MS e FDN apresentaram efeito quadrático (P<0,05) quando foi adicionada CS na dieta. Entretanto, observou-se aumento (P<0,05) da digestibilidade da MS e FDN, enquanto a concentração de ácidos graxos de cadeia curta, acetato e propionato não se alteraram (P>0,05) e o pH ruminal diminuiu (P<0,05). A CS pode substituir parcialmente o milho ou o feno de coastcross sem prejudicar o desempenho ou o metabolismo dos nutrientes de pequenos ruminantes. / The objectives of this experiment were to determine the effects of replacing corn and coastcross hay by soybean hulls (SH) on performance and ingestive behavior in lactating goats and nutrient apparent digestibility and ruminal measures in ram lambs. Experiment 1: Eight goats were housed in a tie stall and used in a two 4 x4 Latin square design conducted concurrently. Each experimental period consisted of 16 d; the first 12 d were used to adapt the goats to the treatments and the remaining 4 d were used for data collection. Animals were fed a 40:60 (concentrate:roughage ratio). Soybean hulls replaced corn by 0%, 20%, 40% or 60% on a dry matter (DM) basis. Dry matter ground intake (DMI), milk yield and ingestive behavior were not affected (P>0.05) by replacing corn by soybean hulls. However, milk fat concentration increased (P<0.05) with soybean hulls inclusion. Experiment 2: Four ram lambs were placed individually in metabolism crates and assigned to a 4 x 4 Latin square design. The experimental period consisted of 14 d; the first 10 d were used to adapt the lambs to the treatments and the remaining 4 d were used for data collection. The treatments and diets were the same as in Experiment 1. Dry matter intake and digestibility were not affected (P>0.05) by replacing ground corn by soybean hulls. Nevertheless, propionate concentration decreased (P<0.05) while neutral detergent fiber (NDF) intake and digestibility, ruminal pH and acetate concentration increased (P<0.05) with soybean hulls inclusion. Experiment 3: Thirty-six goats were assigned to a complete randomized block design and housed in a tie stall for a period of 8 weeks. Goats were fed a 50:50 (concentrate:roughage ratio) with similar amount of NDF. Soybean hulls replaced hay by 0%, 33%, 67% or 100% on a DM basis. Dry matter and NDF intake showed a quadratic response (P>0.05). Milk yield and body weight change didnt differ (P<0.05), however milk fat concentration increased (P>0.05) while time spent with rumination and chewing decreased (P>0.05), when SH were added to the diet. Experiment 4: Sixteen ram lambs were housed individually in metabolism crates and assigned to a complete randomized block design. The experimental period consisted of 14 d; the first 10 d were used to adapt the lambs to the treatments and the remaining 4 d were used for data collection. The treatments and diets were the same used in Experiment 4. DMI and NDF intake showed a quadratic effect (P<0.05) when SH were added to the diet. However, DM and NDF digestibility increased (P<0.05) while short-chain fatty acids, acetate and propionate concentration, didnt change (P>0.05) and ruminal pH decreased (P<0.05). SH can partially replace corn or coastcross hay with no detrimental effects on performance or nutrient metabolism in small ruminants.

Ho, Sai Man. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 144-164). / Abstracts in English and Chinese. / TABLE OF CONTENTS --- p.ii / ABSTRACT --- p.xii / 撮要 --- p.xv / LIST OF ABBREIVIATIONS --- p.xvi / LIST OF TABLES --- p.xvii / LIST OF FIGURES --- p.xix / Chapter Chapter 1 --- General Introduction / Chapter 1.1 --- Overview of lectins --- p.1 / Chapter 1.1.1 --- History of lectins --- p.1 / Chapter 1.1.2 --- Definitions of lectins --- p.2 / Chapter 1.1.3 --- Classification and nomenclature of lectins based on structure --- p.2 / Chapter 1.1.4 --- Classification and nomenclature of lectins based on carbohydrate-bindingspecificity --- p.4 / Chapter 1.1.5 --- Structure of plant lectins --- p.4 / Chapter 1.1.6 --- Biological function of plant lectins --- p.5 / Chapter 1.1.6.1 --- Anti-viral activity of plant lectiins --- p.5 / Chapter 1.1.6.2 --- Lectins as plant defense proteins --- p.6 / Chapter 1.1.6.3 --- Insecticidal activity of plant lectins --- p.7 / Chapter 1.1.6.4 --- Anti-fungal activity of plant lectins --- p.7 / Chapter 1.1.6.5 --- Mitogenic activity of plant lectins --- p.7 / Chapter 1.1.6.6 --- Anti-tumor and anti-proliferative activity of plant lectins --- p.9 / Chapter 1.1.7 --- Background of legume lectins --- p.11 / Chapter 1.1.7.1 --- Structure of legume lectins --- p.11 / Chapter 1.1.7.2 --- Functions and activities of legume lectins --- p.12 / Chapter 1.2 --- Overview of serine protease inhibitors in plants --- p.14 / Chapter 1.2.1 --- Classification of serine protease inhibitor --- p.15 / Chapter 1.2.2 --- The main functions of plant serine protease inhibitors --- p.17 / Chapter 1.2.3 --- Commercial application of serine protease inhibirtors --- p.19 / Chapter 1.2.3.1 --- Medical application --- p.19 / Chapter 1.2.3.2 --- Transgenic application in agriculture --- p.22 / Chapter 1.3 --- Overview of Pathogenesis-related proteins in plants --- p.25 / Chapter 1.3.1 --- Overview of PR-5 family Thaumatin-like proteins (TLPs) --- p.27 / Chapter 1.3.1.1 --- Structural similarities among TLPs --- p.28 / Chapter 1.3.1.2 --- Antifungal activity of TLP --- p.31 / Chapter 1.3.2 --- Overview of Chinase-like proteins (CLPs) --- p.33 / Chapter 1.3.2.1 --- Classification of chitinase --- p.34 / Chapter 1.3.2.1.1 --- On the basis of amino acid sequence of glycosyl hydrolase --- p.34 / Chapter 1.3.2.1.2 --- On the basis of amino acid sequence of plant chitinase --- p.35 / Chapter 1.3.2.2 --- Antifungal activity of CLP --- p.36 / Chapter 1.3.3 --- Anti-freeze property of PR proteins --- p.38 / Chapter 1.3.4 --- Application of PR proteins in agriculture --- p.40 / Chapter 1.4 --- Rationale of the present study --- p.42 / Chapter Chapter 2 --- Materials and Methods / Chapter 2.1 --- Materials --- p.43 / Chapter 2.2 --- Preparation of crude extract --- p.44 / Chapter 2.2.1 --- Hokkaido large black soybean --- p.44 / Chapter 2.2.2 --- Emperor banana --- p.45 / Chapter 2.3 --- Purification --- p.45 / Chapter 2.4 --- Chromatography --- p.46 / Chapter 2.4.1 --- DEAE-cellulose chromatography --- p.46 / Chapter 2.4.2 --- Affi-gel Blue gel --- p.47 / Chapter 2.4.3 --- SP-Sepharse --- p.48 / Chapter 2.4.4 --- Mono Q HR 5/5 and Mono S HR 5/5 --- p.49 / Chapter 2.4.5 --- Superdex 75 and superdex 200 --- p.50 / Chapter 2.5 --- Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) --- p.50 / Chapter 2.6 --- Protein concentration determination --- p.54 / Chapter 2.7 --- Preparation of rabbit reticulocyte lysate --- p.54 / Chapter 2.8 --- Determination of N-terminal amino acid sequence --- p.56 / Chapter 2.9 --- Assay of inhibition of hemagglutinating activity by different carbohydrates --- p.56 / Chapter 2.10 --- Thermal stability determination assays --- p.57 / Chapter 2.10.1 --- Stability at various temperatures --- p.57 / Chapter 2.10.2 --- Stability at 100°C --- p.57 / Chapter 2.11 --- Assay of pH dependence of hemagglutinating activity --- p.58 / Chapter 2.12 --- Assay of ion dependence of hemagglutinating activity --- p.58 / Chapter 2.13 --- Assay of antifungal activity --- p.58 / Chapter 2.14 --- Assay of trypsin inhibitory activity --- p.60 / Chapter 2.15 --- Assay of antibacterial activity --- p.61 / Chapter 2.16 --- Assay for cytotoxic activity on cancer cell lines --- p.61 / Chapter 2.17 --- Assay for HIV-1 reverse transcriptase (RT) inhibitory activity --- p.62 / Chapter 2.18 --- Assay of mitogenic activity --- p.63 / Chapter Chapter 3 --- Purification and Characterization of Defense-Related Proteins from their Respective Sources / Chapter 3.1 --- Purification and Characterization of a Lectin from the Seeds of Hokkaido large black soybean / Chapter 3.1.1 --- Introduction --- p.65 / Chapter 3.1.2 --- Results --- p.66 / Chapter 3.1.3 --- Purification --- p.68 / Chapter 3.1.3.1 --- Affinity chromatography on Affi-gel Blue gel --- p.69 / Chapter 3.1.3.2 --- Anion-exchange chromatography on DEAE-cellulose --- p.70 / Chapter 3.1.3.3 --- Anion-exchange chromatography on Mono Q column --- p.71 / Chapter 3.1.3.4 --- Gel filtration on Superdex 200 column --- p.72 / Chapter 3.1.3.5 --- Hemagglutinating activity at each purification step --- p.73 / Chapter 3.1.4 --- Characterization of Lectin --- p.74 / Chapter 3.1.4.1 --- Molecular mass determination --- p.74 / Chapter 3.1.4.2 --- N-terminal amino acid sequencing --- p.76 / Chapter 3.1.4.3 --- Assay of inhibition of hemagglutinating activity by different carbohydrates --- p.77 / Chapter 3.1.4.4 --- Thermal stability --- p.78 / Chapter 3.1.4.5 --- Assay of pH dependence of hemagglutinating activity --- p.80 / Chapter 3.1.4.6 --- Assay of ion dependence of hemagglutinating activity --- p.81 / Chapter 3.1.4.7 --- Assay for HIV-1 reverse transcriptase (RT) inhibitory activity --- p.82 / Chapter 3.1.4.8 --- Assay of mitogenic activity --- p.83 / Chapter 3.1.4.9 --- Assay of antibacterial activity --- p.84 / Chapter 3.1.5 --- Discussion --- p.86 / Chapter 3.2 --- Purification and Characterization of a Trypsin inhibitor from the Seeds of Hokkaido large black soybean / Chapter 3.2.1 --- Introduction --- p.93 / Chapter 3.2.2 --- Results --- p.94 / Chapter 3.2.3 --- Purification --- p.95 / Chapter 3.2.3.1 --- Anion-exchange chromatography on Mono Q column --- p.96 / Chapter 3.2.3.2 --- Gel filtration on Superdex 75 column --- p.98 / Chapter 3.2.3.3 --- Trypsin inhibitory activity at each purification step --- p.99 / Chapter 3.2.4 --- Characterization of trypsin inhibitory --- p.100 / Chapter 3.2.4.1 --- Molecular mass determination --- p.100 / Chapter 3.2.4.2 --- N-terminal amino acid sequencing --- p.102 / Chapter 3.2.4.3 --- Assay for HIV-1 reverse transcriptase (RT) inhibitory activity --- p.103 / Chapter 3.2.4.4 --- Antiproliferative effect on MCF-7 and Hep G2 cells --- p.104 / Chapter 3.2.4.5 --- pH and thermal stability --- p.105 / Chapter 3.2.5 --- Discussion --- p.106 / Chapter 3.3 --- Purification and Characterization of a Thaumatin-like protein and Chitinase-like protein from Emperor Banana / Chapter 3.3.1 --- Introduction --- p.108 / Chapter 3.3.2 --- Results --- p.109 / Chapter 3.3.3 --- Purification --- p.111 / Chapter 3.3.3.1 --- Affinity chromatography on Affi-gel Blue gel --- p.112 / Chapter 3.3.3.2 --- Cation exchange chromatography on Mono S column --- p.113 / Chapter 3.3.3.3 --- Gel filtration on Superdex 75 column --- p.114 / Chapter 3.3.3.3.1 --- Fraction MS 2 --- p.114 / Chapter 3.3.3.3.2 --- Fraction MS 4 --- p.115 / Chapter 3.3.3.3.3 --- Fraction MS 5 --- p.118 / Chapter 3.3.4 --- Characterization of the thaumatin-like protein --- p.121 / Chapter 3.3.4.1 --- N-terminal amino acid sequence determination --- p.121 / Chapter 3.3.4.2 --- Assay for antifungal activity --- p.122 / Chapter 3.3.4.3 --- Thermal stability --- p.124 / Chapter 3.3.4.4 --- pH stability --- p.125 / Chapter 3.3.4.5 --- Resistance to trypsin digestion --- p.125 / Chapter 3.3.4.6 --- Anti-HIV-1 reverse transcriptase activity --- p.126 / Chapter 3.3.4.7 --- Discussion --- p.127 / Chapter 3.3.5 --- Characterization of the two chitinase-like protein --- p.131 / Chapter 3.3.5.1 --- N-terminal amino acid sequence determination --- p.131 / Chapter 3.3.5.1.1 --- Emperor banana MS2 CLP --- p.131 / Chapter 3.3.5.1.2 --- Emperor banana MS4 CLP --- p.132 / Chapter 3.3.5.2 --- Assay for antifungal activity --- p.133 / Chapter 3.3.5.3 --- Discussion --- p.136 / Chapter Chapter 4 --- general discussion --- p.138 / References --- p.144

The subsector of Brazilian agribusiness has a highlight and growth scenario in GDP composition. Culture and soybean trade in grains have shown performance in the Brazilian economy, representing the trade 27 % of exports in the country. Farming soybeans, in the perception of society, government and the owners, is known to have a high degree of Wealth, understood by the fact that their activity attract jobs, income sources and improved quality of services. In addition, we identified, in theory, instruments that allow measuring the value of this wealth and its distribution. The objective of this work is to understand the process of evolution and wealth distribution seen in crop soybean in Brazil, understood from 1998 to 2014. To meet these goals, data adequacy was held on Wealth, identifying eleven variables, the time horizon of 17 years. The study was delimited to five producing cities represented in the soybean cultivation in the South and Midwest. The analysis presents descriptive and quantitative, has been carried out in two steps: the creation of the comparative examination of the evolution of elements of Wealth by Analysis of Variance and subsequently the bivariate correlation, evaluating the membership level of the variable results with other data. The results showed that the Wealth Generated, costs Seeds and Pesticides, Equity Compensation and the results have similar characteristics in major producing regions when evaluated the average values. The relevance of this study is the theoretical contribution highlighting elements that make up the Value Added cultivation of soybeans. / O subsetor do agronegócio brasileiro apresenta um cenário de crescimento e destaque na composição do Produto Interno Bruto. A cultura e o comércio de soja em grãos têm apresentado um desempenho na economia brasileira, representando o comércio em 27% das exportações no país. A atividade agrícola da sojicultora, na percepção da sociedade, do governo e dos proprietários, é conhecida por apresentar um elevado grau de Riqueza, compreendida pelo fato de sua atividade atrair empregos, fontes de renda e melhoria na qualidade de serviços. Além disso, foram identificados, na teoria, instrumentos que possibilitam mensurar o valor dessa Riqueza e a sua forma de distribuição. O objetivo deste trabalho é a compreensão do processo de evolução e de distribuição de riqueza observado na cultura agrícola de soja no Brasil, compreendido no período de 1998 a 2014. Para atender a esses propósitos, foi realizada adequação de dados sobre a Riqueza, identificando-se onze variáveis, no horizonte temporal de 17 anos. O estudo foi delimitado a cinco cidades produtoras com representatividade no cultivo de soja na Região Sul e Centro-Oeste. A análise apresenta caráter descritivo e quantitativo, tendo sido realizada em duas etapas: a realização do exame comparativo dos elementos de evolução da Riqueza por meio da Análise de Variância e, posteriormente, a Correlação Bivariável, avaliando-se o nível de associação da variável Resultado com os demais dados. Os resultados apontaram que a Riqueza Gerada, Custos com Sementes e Agrotóxicos, Remuneração de Capital Próprio e o Resultado possuem características semelhantes nas grandes regiões produtoras quando avaliados o valores médios. A relevância deste estudo está na contribuição teórica que evidencia elementos que configuram o Valor Adicionado do cultivo da soja em grão. / Mestre em Ciências Contábeis

Submitted by Cristiano Daimond Gomes (cristianodgomes@gmail.com) on 2014-02-28T14:21:40Z No. of bitstreams: 1 Cristiano.Gomes.2014_02_18.pdf: 300845 bytes, checksum: d30881b36322c420328af2824f18ebb2 (MD5) / Approved for entry into archive by Suzinei Teles Garcia Garcia (suzinei.garcia@fgv.br) on 2014-02-28T14:33:44Z (GMT) No. of bitstreams: 1 Cristiano.Gomes.2014_02_18.pdf: 300845 bytes, checksum: d30881b36322c420328af2824f18ebb2 (MD5) / Made available in DSpace on 2014-02-28T15:10:33Z (GMT). No. of bitstreams: 1 Cristiano.Gomes.2014_02_18.pdf: 300845 bytes, checksum: d30881b36322c420328af2824f18ebb2 (MD5) Previous issue date: 2014-01-30 / Nas últimas décadas, o agronegócio brasileiro tem ganhado cada vez mais relevância para a economia nacional, apresentando significativos ganhos de produtividade. Estes ganhos ocorreram devido a maior absorção de tecnologia pelos produtores agrícolas, incluindo o uso de insumos, dentre os quais se destacam os fertilizantes. As vendas de fertilizantes no Brasil cresceram 5,9% entre 1991 e 2012, atingindo 29,5 milhões de toneladas em 2012. Apesar disso, no Brasil, poucos trabalhos tratam dos fatores que afetam as suas vendas. Justifica-se, assim o presente estudo que tem como objetivo analisar os principais fatores que afetam as vendas brasileiras de fertilizantes para a soja. A metodologia utilizada é a de séries temporais e o período da análise é o de 1988 a 2012. Para atingir o objetivo uma função de vendas foi estimada considerando as propriedades de integração e cointegração das séries temporais. O modelo proposto para explicar as vendas de fertilizantes para a soja incluíam as seguintes variáveis exógenas: área plantada de soja; preço dos fertilizantes para a soja; produtividade da soja e preço da soja. A variável preço da soja apresentou uma alta multicolinearidade com a área plantada de soja, e por isto não foi incluída no modelo final. Os resultados encontrados no estudo mostram que a área plantada é o fator que exerce maior influência sobre as vendas de fertilizantes para a soja, sendo que um choque positivo de 1% sobre o crescimento da área plantada tem efeito positivo de 1,8% sobre as vendas de fertilizantes para a soja. O preço dos fertilizantes para a soja resultou em um coeficiente negativo conforme era esperado. A produtividade da soja foi utilizada como uma proxy para a capitalização do produtor agrícola, e, assim como a área plantada, ela apresentou sinal positivo, embora de menor magnitude e não estatisticamente significativo. / In recent decades the Brazilian agribusiness has increasing its importance for the national economy showing significant increases in productivity. These gains in productivity were due to greater diffusion of the use of technology by farmers, including an intensification of the inputs used, among which stand out the fertilizer. Between 1991 and 2012, sales of fertilizers in Brazil grew 5.9%, reaching 29.5 million tons in 2012. Despite the huge growth in sales little has been studied about the factors that affect the sales of fertilizers in Brazil. The aim of this study was to analyze the main factors that affected sales of fertilizers for soybeans in Brazil in the period 1988 to 2012. To achieve this goal a function of demand was estimated considering the properties of integration and co - integration of time series. The proposed model specified the sales of fertilizers for soybeans from the following exogenous variables: soybean acreage, fertilizer prices for soybeans, soybean yield, soybean prices. The variable price of soybeans showed high multicollinearity with the soybean acreage, and therefore was not included in the final model. The results from the final model showed that acreage is the factor that has the most influence on the sales of fertilizers for soybeans, with a positive 1% shock on growth of planted area effect 1.8% growth on sales fertilizer for soybeans. The price of fertilizer for soybeans resulted in a negative coefficient as expected. Soybean yield was used as a proxy for the capitalization of the agricultural producer and acreage she presented positive sign, although of lesser magnitude compared to acreage, but the coefficient was not significant.