Neste trabalho, estudou-se a decomposição térmica dos edulcorantes não calóricos acessulfame-K, aspartame, ciclamato de sódio, esteviosídeo, sacarina e sacarina sódica de crescente uso na indústria alimentícia. Para tanto, essas amostras foram submetidas à análise por TG, DTG DTA e DSC, após caracterização por análise elementar e por IV. Após recristalização dos compostos comerciais ciclamato de sódio e sacarina sódica, observou-se nas curvas TG a presença de águas de hidratação no aspartame e na sacarina sódica. Nesta última, a primeira água é muito volátil e pode ser eliminada na secagem. O esteviosídeo apresentou umidade. Os intermediários e os resíduos dos mesmos foram caracterizados por IV e quando necessário por difração de raios X. De maneira geral os compostos apresentaram decomposição com formação de resíduo carbonizado e geração de sulfato de potássio, no caso do acessulfame-K e sulfato de sódio na sacarina e ciclamato de sódio. Estudos de cinética de decomposição em fase sólida foram realizados para o aspartame e sacarina, pois estes não apresentavam sobreposição de eventos. Os estudos cinéticos para decomposição térmica usando o método de Flynn-Wall-Ozawa só puderam ser realizados para o aspartame resultando em log A = 23,2 +-0,1 min-1 e Ea = 206,6 +- 0,3 KJ mol-1. Os demais compostos apresentaram decomposição por eventos simultâneos, que dificultam os cálculos cinéticos. A sacarina H apresentou volatilização após fusão, com Ea = 154,9 +- 0,7 KJ mol-1 e log A = 15,2 +- 0,1 min-1. / In this work the thermal decomposition of the non-caloric sweeteners acesulfame-K, aspartame, sodium ciclamate, stevioside, saccharine and sodium saccharine was performed. Initially, the compounds were characterized usually elemental analysis and IR spectroscopy some were recrystallised from commercial samples, resulting in purified compounds. After characterization the sweetener were analysed by TG/DTG, DTA and DSC. The recrystallised compounds presented hydration water in aspartame and sodium saccharine. In the last the first water can be lost during drying, since it seems to be weakely bounded. Stevioside showed humidity water. In a general way the sweeteners decomposes by a carbonaceous residue, and formed potassium sulfate as final residue in the acesulfame-K case as well as sodium sulfate in sodium saccharine and sodium ciclamate. Due to the overlapping of consecutive decomposition steps. The use o the Flynn-Wall-Ozawa method for kinetic calculations only could be performed for the decomposition of aspartame, resulting in log A = 23,2 +-0,1 min-1 and Ea = 206,6 +-0,3 KJ mol-1. The volatilization of saccharine H, after fusion, resulted in Ea = 154,9 +-0,7 KJ mol-1 and log A = 15,2 +-0,1 min-1.

A crescente preocupação dos efeitos da ingestão de açúcar sobre a saúde tem levado a um aumento acentuado no consumo de adoçantes artificiais (ADDs). Ao mesmo tempo em que se nota elevada prevalência de obesidade, há também um aumento expressivo no número de produtos alimentícios com adoçantes artificiais. Objetivo. Caracterizar o perfil e analisar a atitude em relação ao consumo de ADD e alimentos dietéticos (ALDs) e sua relação com o excesso de peso corporal. Métodos. Estudo transversal com indivíduos com idades 20 anos, funcionários e docentes de universidades públicas do estado de São Paulo. Foi aplicado um questionário online com perguntas quanto ao uso de ADD e ALD e informações demográficas, socioeconômicas, antropométricas e de saúde. Verificaram-se associações entre as variáveis sócio-demográficas e antropométricas e o consumo de ADDs e ALD; utilizou-se análise de cluster para o reconhecimento de grupos homogêneos e regressão logística binária com cálculo do Odds-Ratio (OR). Resultados. Participaram do estudo 1323 indivíduos. A prevalência de uso de adoçante dietético foi 53,3 por cento , sendo maior entre aqueles com 30 a 60 anos. O peso médio foi de 72,03 kg e o IMC médio 25,26 kg/m2. As principais motivações foram a dificuldade de manter o peso corporal, não querer engordar e querer economizar calorias para poder consumir outros alimentos. A maioria possui o hábito de utilizar o adoçante na forma líquida, e os mais consumidos são sacarina e ciclamato de sódio, aspartame e sucralose. A quantidade média para adoçar uma porção de líquidos foi de 5,0 gotas. Em relação ao consumo de ALDs, a prevalência foi de 52,5 por cento , sendo refrigerantes, sucos de fruta, barras de cereal e iogurtes. No modelo de regressão logística, as variáveis associadas com o consumo de ADDs foram sexo, idade, DM, dificuldade em manter o peso e consumo de alimentos dietéticos. Para os ALDs, os fatores associados foram renda, sexo, DM, dificuldade em manter o peso, frequência da prática de atividade física e consumo de ADDs. Conclusão. O perfil do consumidor de adoçantes e alimentos dietéticos é formado principalmente por indivíduos com excesso de peso, dificuldade em manter o peso, não diabéticos e não hipertensos. Tanto para ADD como para ALD, a maior chance de consumi-los está entre aqueles que buscam esses produtos para controle do peso corporal ou do DM. Os indivíduos com excesso de peso e que possuem dificuldade para manter o peso corporal consomem mais adoçantes em relação aos eutróficos. / The growing concern of the effects of sugar intake on health has led to a sharp increase in the consumption of artificial sweeteners (ADDs). At the same time that the high prevalence of obesity, we note there is also a significant increase in the number of food products with artificial sweeteners. Objective. To characterize the profile and analyze the attitude towards the consumption of ADD and diet foods (ALDs) and its relationship with excess body weight. Methods. Cross sectional study with individuals aged 20 years, employees and professors of public universities in the state of São Paulo. An online questionnaire was applied with questions about the use of ADD and ALD and sociodemographic, anthropometric and health information. Associations between socio-demographic and anthropometric variables and the consumption of ADDs and ALD were analyzed. It was used the cluster analysis to the recognition of homogeneous groups and binary logistic regression to calculate the odds ratios (OR). Results. The study included 1323 individuals. The prevalence of use of artificial sweetener was 53.3 per cent , being higher among those aged 30 to 60 years. The mean weight was 72.03 kg and the mean BMI 25.26 kg / m2. The main reasons to consume ADD were the difficulty to maintain body weight, \"not wanting to get fat\" and \"want to save calories to be able to consume other foods.\" Most have the habit of using the sweetener in liquid form, and the most consumed sweeteners were saccharin and sodium cyclamate, aspartame and sucralose. The average amount to sweeten a portion of liquid was 5.0 drops. Regarding use of ALDs, the prevalence was 52.5 per cent , and soft drinks, fruit juices, cereal bars and yoghurts. In the logistic regression model, the variables associated with the consumption of ADDs were gender, age, DM, difficulty maintaining weight and consumption of diet foods. For ALDs, associated factors were income, gender, DM, difficulty maintaining weight, frequency of physical activity and consumption of ADDs. Conclusion. The consumer profile of artificial sweeteners and diet foods is mainly formed by individuals with overweight, difficulty maintaining weight, non-diabetic and non-hypertensive. Both for ADD and for ALD, the greater chance of consuming them is between those who seek these products to control weight or DM. Individuals who are overweight and have difficulty maintaining body weight consume more ADD than normal individuals.

Inledning: Tv-reklam för söta drycker kan leda till ökad konsumtion av ohälsosamma och energirika drycker med högt sockerinnehåll. Under år 2014 inhandlades i genomsnitt 92 liter läskedryck i Sverige per konsument, vilket är en ökning med tio liter jämfört med år 2000. Vid regelbunden konsumtion av söta drycker ökar risken för att utveckla såväl övervikt och fetma som karies. Syfte: Syftet är att undersöka om antalet reklaminslag för söta drycker på tv har förändrats under åren 1999-2014 och om det finns några skillnader mellan olika tv-kanaler när det gäller antalet reklaminslag. Material och metod: I studien undersöks tv-reklam för de söta dryckerna läsk, saft och juice med utgångspunkt från ett Exceldokument baserat på reklamdata från TvChecks databas. Genom att filtrera data efter livsmedelsnummer och år kunde olika tabeller och diagram skapas för att se vilka förändringar som skett i antalet reklaminslag för söta drycker under åren 1999-2014. Resultat: Tv-reklam för söta drycker har ökat markant mellan åren 2013 och 2014. De tv-kanaler som i genomsnitt visat mest reklam för söta drycker är Kanal 5 och TV3, samtidigt som TV4 och TV7 sänt minst tv-reklam i genomsnitt under åren 1999-2014. Utöver detta syns en tydlig ökning av antalet reklaminslag för drycker sötade med sötningsmedel under perioden 1999-2014 från 586 till 8716 reklaminslag. Slutsats: Antalet reklaminslag för söta drycker har ökat markant under åren 2013-2014, samtidigt som antalet reklaminslag under åren 1999-2009 legat på en relativt konstant nivå. / Introduction: TV commercials for sweetened drinks may result in an increased consumption of unhealthy and energy dense drinks with a high content of sugar. In 2014 the swedes purchased an average of 92 litres of soft drinks per consumer, which is an increase by ten litres compared to the year 2000. Regular consumption of sugary drinks increases the risk of developing overweight, obesity and caries. Aim: The aim of the study is to investigate whether the number of commercials for sweetened drinks on TV has changed during the years 1999-2014 and if there are any differences between various TV channels regarding the number of commercials. Material and method: The study examines TV commercials for sweetened drinks such as juice and soft drinks (with and without carbonic acid) using an Excel document based on data from TvChecks database. By filtering the data by different food numbers and year various tables and graphs were created to show what changes occurred in the number of commercials for sweetened drinks during the years 1999-2014. Results: TV commercials for sweetened drinks has increased largely between the years 2013 and 2014. The TV channels that in average showed the most commercials for sweetened drinks are the Swedish Channel 5 and TV3, while TV4 and TV7 showed the least commercials in average during the years 1999-2014. Moreover, the TV commercials for drinks sweetened with sweeteners shows a clear increase during the years 1999-2014 from 586 to 8716 commercials. Conclusion: The number of TV commercials for sweetened drinks has increased considerably over the years 2013-2014, while the number of commercials during the years 1999-2009 was relatively constant.

Com o intuito de verificar a eficácia do açúcar e de edulcorante a base de sacarina sódica e neoesperidina nas dietas de leitões, foram realizados dois experimentos, ambos com 42 leitões recém-desmamados aos 21 dias de idade. Foram oferecidas três dietas: dieta controle (DC), dieta com açúcar (DA) e dieta com edulcorante (DE). Os experimentos foram em blocos causalizados, sendo o primeiro com sete repetições e dois animais por unidade experimental e o segundo com seis repetições, um animal por unidade experimental e arranjo fatorial 3 x 2 + 1 (três dietas, duas épocas de abate: aos 28 e 35 dias de idade e o abate no dia do desmame). No primeiro experimento, os animais permaneceram nas instalações de creche até os 63 dias de idade para avaliação do desempenho, da incidência de diarreia e da viabilidade econômica das dietas. No segundo experimento, os animais foram abatidos para se proceder à pesagem de órgãos, medição do comprimento do intestino delgado e coleta dos segmentos do duodeno e jejuno para realização da morfometria intestinal. Não houve diferenças entre as dietas para o CDR, GDP e CA nos Períodos 1 (21 aos 28 dias), 3 (21 aos 50 dias) e 4 (21 aos 63 dias de idade). No Período 2 (21 aos 35 dias), os animais que consumiram a DE apresentaram maior (P<0,05) CDR e GDP, se comparados aos que consumiram a DA. Nos Períodos 1, 2 e 3 foi observado menor custo da dieta por kg de peso vivo ganho e melhores índices econômico e de custo para os animais que consumiram DE. Ao avaliar a morfometria intestinal e peso de órgãos, não se observou diferença entre as dietas, com exceção para o peso do estômago, que foi maior nos animais que consumiram DCE. A inclusão de edulcorante é eficaz em melhorar o desempenho no Período 2, além de ser viável sob o ponto de vista econômico. / In order to verify the effectiveness of sugar and sweetener containing saccharin and neohesperidin in diets of piglets, two experiments were conducted, both with 42 newborn piglets weaned at 21 days old. Were offered three diets: control diet (DC), diet with sugar (DA) and diet with sweetener (DE). The experiments were in randomized blocks, the first of which had seven replicates and two animals per experimental unit and the second, six replicates and one animal per experimental unit comprising a factorial 3 x 2 + 1 (three diets, two slaughter ages: at 28 and 35 days of age and slaughter on the day of weaning). In the first experiment, the animals remained on the nursery facilities until 63 days of age to evaluate the performance, the incidence of diarrhea and the economic viability of the diets. In the second experiment, the animals were slaughtered to weigh the organs, measure the length of the small intestine and collecting segments of the duodenum and jejunum to determine intestinal morphology. There were no differences between diets for the ADFI, FC and ADG in the Periods 1 (21 to 28 days), 3 (21 to 50 days) and 4 (21 to 63 days old). In the Period 2 (21 to 35 days), animals that consumed the DE had higher (P<0.05) ADFI and ADG, compared to those fed the DA. In Periods 1, 2 and 3 was observed lower cost per kg of the diet of live weight gain and better economic indicators to the animals fed DE. No differences were observed between the diets when evaluating intestinal morphology and organ weights, except for the weight of the stomach, which was higher in animals fed DE. The inclusion of sweetener is effective in improving performance in Period 2, and is viable under the economical point of view.

Com o intuito de verificar a eficácia do açúcar e de edulcorante a base de sacarina sódica e neoesperidina nas dietas de leitões, foram realizados dois experimentos, ambos com 42 leitões recém-desmamados aos 21 dias de idade. Foram oferecidas três dietas: dieta controle (DC), dieta com açúcar (DA) e dieta com edulcorante (DE). Os experimentos foram em blocos causalizados, sendo o primeiro com sete repetições e dois animais por unidade experimental e o segundo com seis repetições, um animal por unidade experimental e arranjo fatorial 3 x 2 + 1 (três dietas, duas épocas de abate: aos 28 e 35 dias de idade e o abate no dia do desmame). No primeiro experimento, os animais permaneceram nas instalações de creche até os 63 dias de idade para avaliação do desempenho, da incidência de diarreia e da viabilidade econômica das dietas. No segundo experimento, os animais foram abatidos para se proceder à pesagem de órgãos, medição do comprimento do intestino delgado e coleta dos segmentos do duodeno e jejuno para realização da morfometria intestinal. Não houve diferenças entre as dietas para o CDR, GDP e CA nos Períodos 1 (21 aos 28 dias), 3 (21 aos 50 dias) e 4 (21 aos 63 dias de idade). No Período 2 (21 aos 35 dias), os animais que consumiram a DE apresentaram maior (P<0,05) CDR e GDP, se comparados aos que consumiram a DA. Nos Períodos 1, 2 e 3 foi observado menor custo da dieta por kg de peso vivo ganho e melhores índices econômico e de custo para os animais que consumiram DE. Ao avaliar a morfometria intestinal e peso de órgãos, não se observou diferença entre as dietas, com exceção para o peso do estômago, que foi maior nos animais que consumiram DCE. A inclusão de edulcorante é eficaz em melhorar o desempenho no Período 2, além de ser viável sob o ponto de vista econômico. / In order to verify the effectiveness of sugar and sweetener containing saccharin and neohesperidin in diets of piglets, two experiments were conducted, both with 42 newborn piglets weaned at 21 days old. Were offered three diets: control diet (DC), diet with sugar (DA) and diet with sweetener (DE). The experiments were in randomized blocks, the first of which had seven replicates and two animals per experimental unit and the second, six replicates and one animal per experimental unit comprising a factorial 3 x 2 + 1 (three diets, two slaughter ages: at 28 and 35 days of age and slaughter on the day of weaning). In the first experiment, the animals remained on the nursery facilities until 63 days of age to evaluate the performance, the incidence of diarrhea and the economic viability of the diets. In the second experiment, the animals were slaughtered to weigh the organs, measure the length of the small intestine and collecting segments of the duodenum and jejunum to determine intestinal morphology. There were no differences between diets for the ADFI, FC and ADG in the Periods 1 (21 to 28 days), 3 (21 to 50 days) and 4 (21 to 63 days old). In the Period 2 (21 to 35 days), animals that consumed the DE had higher (P<0.05) ADFI and ADG, compared to those fed the DA. In Periods 1, 2 and 3 was observed lower cost per kg of the diet of live weight gain and better economic indicators to the animals fed DE. No differences were observed between the diets when evaluating intestinal morphology and organ weights, except for the weight of the stomach, which was higher in animals fed DE. The inclusion of sweetener is effective in improving performance in Period 2, and is viable under the economical point of view.

The theoretical part of the diploma thesis deals with cultivation of the plant Stevia rebaudiana. This plant is 300x sweeter than sucrose. It is often used as a compensation for sugar sweetener, suitable for diabetics and people who suffer from overweight. There is processed the characteristics of the plant, botanical description, conditions of cultivation and chemical composition. There are described the methods of determination of steviol glycosides. The practical part elaborates on a sensorical analysis. This analysis has two parts. The first part is the evaluation of stevia concentrates with the flavour of aronia, sour cherry and beetroot and stevia powder in 10 milk products bought in supermarkets. The second part deals with the evaluation of black tea sweetened with few different easily available sorts of sweetening products. The currently valid legislative in the area of the Czech Republic allows the food industry to use steviol glycosides of high purity under the name E960. The use of the plant stevia or its parts as food isn't enabled.

Many factors influence the efficiency of muscle growth including genetics, nutrition, and environment. The neonatal period is characterized as a time of rapid growth. Growth rate is reduced during neonatal nutrient restriction possibly due to altered satellite cell activity which can permanently alter growth potential. Therefore, optimal nutrition is important for maximizing the growth potential of the animal. Heat stress leads to changes in digestion and metabolism, thus alters nutrient availability to muscle. Heat stress is a prevalent problem in the agriculture industry resulting in great economic losses due to reduced growth, fertility, and increased morbidity. The use of functional feed additives is a potential strategy to mitigate these negative effects. The objective of this dissertation was to investigate nutritional strategies to improve growth in pigs during key malleable periods. Three nutritional studies were conducted to discern the optimal inclusion levels of calcium phosphate, energy, and protein in the diet to maximize neonatal muscle growth. Adequate dietary calcium phosphate was most efficient for satellite cell function which may be mediated by micro-RNA. Differentiation promoting miR-206 and correspondingly the fusion rate was highest in adequate calcium phosphate diets. Excess protein diets enhanced body and muscle growth, while deficient protein was detrimental to growth. Dietary protein treatments altered energy metabolism genes, and genes regulating protein degradation were upregulated in deficient protein diets. Dietary energy levels did not influence body weight, however feed efficiency improved with energy balance. Excess energy diets had the lowest fusion rates and the lowest differentiation promoting miR-1 expression. These data suggest that nutrient inclusion levels are important for satellite cell function and may mediate satellite cell activity through the expression of micro-RNAs. The final study sought to discern the ability of supplementation of an artificial high-intensity sweetener and capsicum oleoresin to mitigate the negative effects of heat stress on pig performance. Heat stress leads to increased body temperature and respiration and was detrimental to metabolic flexibility. Supplementation helped improve feed efficiency and maintain metabolic flexibility. These data indicate that supplementation may be an efficient strategy to mitigate heat stress. / Doctor of Philosophy / Muscle is an important tissue to consider when optimizing growing conditions in feed animals due to its function as a consumer good. Many factors influence the efficiency of muscle growth including genetics, nutrition, and environment. Fractional growth rates are highest during the neonatal period and animals require adequate nutrients to facilitate this growth. Nutrient restriction reduces growth rate and can lead to permanent changes the animals' body size and composition later in life. Therefore, optimal nutrition is important for maximizing the growth potential of the animal. While the nutrients in feed can be controlled to improve growth, other factors are more difficult to regulate. Heat stress is a prevalent problem in the agriculture industry resulting in great economic losses due to reduced growth, fertility, and increased morbidity. The use of functional feed additives is a potential strategy to alleviate these negative effects. The objective of this dissertation was to investigate nutritional strategies to improve growth in pigs during key malleable periods. Three nutritional studies were conducted to determine the optimal inclusion levels of calcium phosphate, energy, and protein in the diet to maximize neonatal muscle growth. Satellite cells are muscle-specific stem cells that help facilitate the growth of muscle. Altering the ability of satellite cells to proliferate and fuse impairs the ability of muscle to grow and repair. Adequate dietary calcium phosphate was most efficient for satellite cell function. Excess protein diets enhanced body and muscle growth, while deficient protein was detrimental to growth. Dietary protein treatments altered energy metabolism genes, and genes regulating protein degradation were upregulated in deficient protein diets. Dietary energy levels did not influence body weight, however, feed efficiency improved with energy balance. Satellite cells from excess energy diets had the lowest fusion rates. These data suggest that nutrient inclusion levels are important for satellite cell function and growth. The final study sought to discern the ability of the supplementation of an artificial high-intensity sweetener and capsicum oleoresin to mitigate the negative effects of heat stress on pig performance. Heat stress leads to increased body temperature and respiration and was detrimental to metabolic flexibility. Supplementation helped improve feed efficiency and maintain metabolic flexibility. These data indicate that supplementation may be an efficient strategy to mitigate heat stress.

Stevia or Stevia rebaudiana Bertoni are two of the numerous names of this subtropical plant with a sweetening power as much as 300 times higher than in case of sucrose. Stevia is a plant with a very interesting, but up to this date not very utilised potential. The fields of its application, processing, cultivation and wintering options have not been explored very extensively, and therefore I have decided to elaborate this topic related to the impact of the environmental conditions for growing of Stevia rebaudiana. The resources for the text of the diploma thesis were formed by available specialised publications and the Internet. The first part of the thesis represents a brief overview of the whole issue concerning the topic of Stevia rebaudiana. The objectives, hypotheses and methodology of the conducted research, including the findings based on the plant growing as well, and the evaluation of the experiment results have been included in the second part. The purpose of the thesis was to expand the knowledge regarding cultivation, reproduction methods and wintering options of the stevia plants. A field and a greenhouse experiments were staged with a focus on temperature indicators during the growing season and their impact on the total number of wintered plants. Simultaneously, an evaluation of the dry matter yield of the plants per an area unit and statistical processing and evaluation of the experiment results have been performed. Further, a determination of seed germination for Stevia rebaudiana in laboratory conditions has been conducted. The research results have shown that the plants obtained vegetatively are more suitable for cultivation due to a higher yield of the dry matter in both greenhouse as well as field conditions. The only pests invading stevia were the whiteflies appearing only in the greenhouse environment. Variable conditions were arranged for the wintering of the Stevia rebaudiana plants within the experiment; and in spite of the various materials and thermal insulation layers, the plants have not successfully wintered in an unheated environment. On the contrary, in the heated greenhouse space, most of the plants have successfully wintered. The stevia seed germination was low and proves the opinion of the majority of authors. The dry matter yields also matched the yields mentioned in the specialised literature.

The subject of this diploma thesis is "Assessment of quality selected bakery products for specific nutrition". Theoretical part explains the legislation for bakery products, characterizes the raw materials at tjhe input and the technological process in the production of bakery and the assessment methodes of bakery products. Practical part is focused at sensory analysis of fine pastery products called "vánočka" suitable for diabetics and obtained data are evaluated using appropriate statistical and graphical methodes.

Made available in DSpace on 2014-06-11T19:27:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-02-18Bitstream added on 2014-06-13T20:16:32Z : No. of bitstreams: 1 casarotti_sn_me_sjrp.pdf: 759646 bytes, checksum: 73cf28759e83bc9e7f545a0f187c2bfd (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Os leites fermentados probióticos são líderes no mercado de alimentos funcionais e prioridade de pesquisa em diversos países. A presença de bactérias probióticas viáveis e em alto número no produto durante sua vida de prateleira é condição essencial para assegurar o efeito probiótico. As qualidades tecnológica e funcional de leites fermentados são importantes para sua aceitação pelo consumidor e podem ser afetadas pelos aditivos adicionados antes da fermentação dos produtos. Neste trabalho, o objetivo foi avaliar o efeito da adição de edulcorantes, em diferentes concentrações, na produção de leites fermentados por uma cultura mista composta por Streptococcus thermophilus e Lactobacillus bulgaricus (Rich) e uma cultura pura composta por Lactobacillus acidophilus (La-5). As bases lácteas para preparação dos leites fermentados obtidas antes da fermentação foram submetidas às determinações dos teores de sólidos totais, proteínas, cinzas, gordura, acidez titulável e valor calórico. Nos leites fermentados foram avaliados: a viabilidade das bactérias láticas, a pós-acidificação, a sinérese e a capacidade de retenção de água (CRA), durante 28 dias de estocagem refrigerada. As características físico-químicas dos leites fermentados atenderam aos requisitos legais e apresentaram diferenças somente nos teores de sólidos totais, cinzas e valor calórico nos produtos elaborados com sacarose. O tipo e a concentração de edulcorante afetaram a fermentação da cultura La-5 e a acidez titulável. No entanto, não influenciaram o tempo de fermentação da cultura Rich, a viabilidade de S. thermophilus, L. bulgaricus e L. acidophilus, a sinérese e a CRA dos leites fermentados elaborados. O tipo de cultura influenciou o tempo de fermentação do leite, sendo superiores os tempos obtidos para La-5 e a acidez titulável, com valores superiores para a cultura... / Fermented probiotic dairy products are leaders in the functional food markets and priority for researching worldwide. The presence of probiotic bacteria viable at high level during shelf-life of the product is essential to ensure the probiotic effect. The technological and functional qualities of fermented milks are important for their acceptance by the consumers and they can be affected by the addition of ingredients before fermentation. The aim of this research was to evaluate the use of sweeteners, in different concentrations, in the production of fermented milks using a mixed culture composed of Streptococcus thermophilus and Lactobacillus bulgaricus (Rich), or a pure culture composed of Lactobacillus acidophilus (La-5). The milk bases obtained before fermentation were submitted to determinations of total solids, protein, ash, fat, acidity contents and caloric value. The viability of lactic bacteria, post-acidification, syneresis and waterholding capacity (WHC) of fermented milks were examined during 28 days of cold storage. The physicochemical characteristics of fermented milks were according to legal requirements and showed differences only on the contents of total solids, ash and caloric value in the products formulated with sucrose. The type and concentration of sweeteners affected the fermentation of La-5 culture and titratable acidity. However they did not influence fermentation time of Rich culture, viability of S. thermophilus, L. bulgaricus and L. acidophilus, syneresis and WHC of fermented milks. The culture type affected the fermentation time, which was higher for La-5 culture, and acidity, which was higher for Rich culture, however, they did not influence the syneresis and WHC of fermented milks. All kinds and concentrations of sweeteners did not influence the technological quality of products, so all of them can be used to produce lowcalorie fermented milks.