Submitted by Socorro Pontes (socorrop@ufersa.edu.br) on 2017-09-05T12:40:23Z No. of bitstreams: 1 RaulinoCN_DISSERT.pdf: 941381 bytes, checksum: e4c8162091f05a4a15cd3b57ebcc36e3 (MD5) / Approved for entry into archive by Vanessa Christiane (referencia@ufersa.edu.br) on 2017-09-06T15:20:38Z (GMT) No. of bitstreams: 1 RaulinoCN_DISSERT.pdf: 941381 bytes, checksum: e4c8162091f05a4a15cd3b57ebcc36e3 (MD5) / Approved for entry into archive by Vanessa Christiane (referencia@ufersa.edu.br) on 2017-09-06T15:23:03Z (GMT) No. of bitstreams: 1 RaulinoCN_DISSERT.pdf: 941381 bytes, checksum: e4c8162091f05a4a15cd3b57ebcc36e3 (MD5) / Made available in DSpace on 2017-09-06T15:23:13Z (GMT). No. of bitstreams: 1 RaulinoCN_DISSERT.pdf: 941381 bytes, checksum: e4c8162091f05a4a15cd3b57ebcc36e3 (MD5) Previous issue date: 2017-04-29 / Conselho Nacional de Desenvolvimento Científico e Tecnológico / Wastewater from pisciculture has benn used in irrigation and tested in the production of fruitful seedlings. Seaweeds synthesize plant hormones and there are products based on extracts of macroalgae, which are used commercially as biostimulants to increase agricultural production. The objective of this work was to test the efficiency of the best dose of seaweed extract in the development of tamarind seedlings. The work was carried out, from May to October 2016, at the Federal Rural Semi-Arid University (UFERSA), East Campus, in Mossoró-RN. The substrate used was formulated with 30% manure and 70% soil. The experimental design used was in randomized complete blocks (DBC) in a 2x5 factorial scheme, with four replications, in both experiments: 2 (“Bioestimulante A” – “Bioestimulante B”) x 5 (0 ml L-1, 4 ml L-1, 8 ml L-1, 12 ml L-1 e 16 ml L-1de bioestimulante) on first experiment, on the second, 2 ( “Com Bioestimulante – Sem Bioestimulante”) x 5 (0%, 25%, 50%, 75% e 100% of wastewater from pisciculture). Morphological and physiological characteristics were analyzed. The biostimulant based on A. nodosum (Biostimulant "B"), at the dose 4.0 ml L-1, promoted the greatest increases in the dry biomass of tamarind tree and reached the highest values of total length. Biostimulant based on lithothannium (Bio-A) is superior to biostimulant based on Ascophyllum nodosum (Bio-B), with respect to stomatal conductance and high doses, above 5.54 ml L-1, compromise the photosynthetic rate of tamarind seedlings. High concentrations of wastewater from fish farming promote decline in the following evaluated characteristics: Number of leaves, length of the aerial part, total length, dry mass of the root system, dry mass of the aerial part, total dry mass, stomatal conductance and transpiration. The concentrations used in this experiment (25%, 50%, 75% and 100%) of aquaculture water were very high, not allowing to observe the beneficial effect of water. In this way, we suggest that the next surveys be carried out using smaller concentrations. The biostimulant based on Ascophyllum. nodosum (Biostimulant "B") at the dose 4.0 ml L-1 promoted the greatest increases in the aerial parts and dry biomass of tamarind seedlings / A água residuária da piscicultura vem sendo utilizada para irrigação e tem sido testada na produção de mudas de frutíferas. As algas marinhas sintetizam hormônios vegetais e existem produtos à base de extratos de macroalgas utilizadas comercialmente como bioestimulantes para aumentar a produção agrícola. O objetivo desta pesquisa foi avaliar diferentes concentrações de água residuária da piscicultura e doses de bioestimulantes naturais de algas marinhas na produção de mudas de tamarindeiro (Tamarindus indica L.). O trabalho foi realizado, no período de maio a outubro de 2016, no viveiro de mudas do Setor de Fruticultura da UFERSA, Mossoró – RN. O substrato utilizado foi formulado com 30% de esterco bovino e 70% de solo. O delineamento experimental utilizado foi o “DBC” em esquema fatorial 2x5, com quatro repetições, nos dois experimentos: 2 (“Bioestimulante A” – “Bioestimulante B”) x 5 (0 ml L-1, 4 ml L-1, 8 ml L-1, 12 ml L-1 e 16 ml L-1de bioestimulante) no primeiro experimento, e no segundo, 2 ( “Com Bioestimulante – Sem Bioestimulante”) x 5(0%, 25%, 50%, 75% e 100% de água residuária da piscicultura). Foram realizadas análises das características morfológicas e fisiológicas. O bioestimulante à base de Ascophyllum nodosum (Bioestimulante “B”), na dose 4,0 ml L-1, promoveu os maiores incrementos na biomassa seca de tamarindeiro e atingiu os maiores valores de comprimento total. O bioestimulante à base de lithothannium (Bioestimulante “A”) é superior ao bioestimulante à base de Ascophyllum nodosum (Bioestimulante “B”), na variável de condutância estomática e doses elevadas de bioestimulantes, acima de 5,54 ml L-1, comprometem à taxa fotossintética de mudas de tamarindeiro. Altas concentrações de água residuária da piscicultura promovem declínio nas seguintes características avaliadas: Número de Folhas, Comprimento da parte Aérea, Comprimento Total, Massa Seca do Sistema Radicular, Massa Seca da Parte Aérea, Massa Seca Total, Condutância Estomática e Transpiração. As concentrações utilizadas neste experimento (25%, 50%, 75% e 100%) de água residuária da piscicultura foram bastante elevadas, não permitindo se observar o efeito benéfico da água. Dessa forma, sugerimos que as próximas pesquisas sejam realizadas utilizando concentrações menores. A fertilização com bioestimulante da espécie Ascophyllum. nodosum na dose de 4,0 ml L-1 promove incrementos no diâmetro do colo, comprimento da parte aérea e no acúmulo de biomassa seca de mudas de tamarindeiro / 2017-09-05

Tamarindus indica L. é um componente natural amplamente consumido por humanos, apresentando propriedade antiinflamatória, antidiabética e antihepatotóxica. Além disso, como nós demonstramos previamente, o extrato apresenta atividade hipolipêmica e antioxidante. Neste trabalho, nós demonstramos o efeito do extrato de T. indica sobre mitocôndrias isoladas de fígado de rato. Na presença de Ca2+, o extrato causou um inchamento osmótico mitocondrial concentração-dependente, associado ao aumento da velocidade basal (V4), dissipação do potencial de membrana e liberação do Ca2+ pré-acumulado, os quais foram inibidos por ciclosporina A (CsA) e assim atribuídos a transição de permeabilidade da membrana mitocondrial (TPMM). A indução do inchamento osmótico mitocondrial foi prevenida por EGTA e vermelho de rutênio, indicando a dependência da TPMM de Ca2+. A oxidação de proteínas tiol mitocondrial, um mecanismo bem estabelecido como causador da TPMM, foi detectado. Entretanto, nenhuma alteração significante foi observada no estado redox de GSH. A oxidação de NAD(P)H e o acúmulo de espécies reativas de oxigênio produzidas pela mitocôndria foram observadas, em ambos os casos, foram prevenidas por CsA e/ou EGTA, indicando que são conseqüências da TPMM induzida pelo extrato de T. indica. Portanto, sem aparente envolvimento do estresse oxidativo no processo, sugerindo uma interação direta de compostos do extrato com grupos tiólicos de proteínas da membrana. O extrato de T. indica induziu uma depleção de ATP associada à TPMM, mostrando assim potencial para causar a morte celular por apoptose ou necrose, resultante da indução da TPMM por si ou pela depleção do ATP via TPMM. In vitro, o extrato apresentou capacidade scavenger de radicais livres, verificado pelo ensaio do DPPH, radical superóxido e radical hidroxil; e ainda foi capaz de diminuir a lipoperoxidação mitocondrial. Além disso, em baixas concentrações, o extrato mostrou uma propriedade quelante de Fe2+. / Tamarindus indica L. is a natural dietary component widely consumed by humans, presenting well established anti-inflammatory, anti-diabetic and anti-hepatotoxic properties. In addition, as we have previously demonstrated, extract presents hypolipemic and antioxidant activities. We show here the effects of T. indica extract on isolated rat liver mitochondria. In the presence of Ca2+, the extract caused mitochondrial concentration-dependent swelling, associated to, resting respiration increase (V4), membrane potential dissipation and release of pre-accumulated Ca2+, inhibited by cyclosporine A (CsA) and thus ascribable to mitochondrial permeability transition (MPT). This swelling induction was prevented by EGTA and ruthenium red, indicating strict dependence of MPT on Ca2+. Oxidation of mitochondrial membrane protein thiols, a well established mechanism causing MPT was detected. However, no significant change was observed in the GSH redox state, and the NADPH oxidation and accumulation of mitochondria-generated reactive oxygen species that was observed, were prevented by CsA and/or EGTA, indicating that they are consequence of the MPT induced by T. indica extract. Therefore, no apparent oxidative stress condition is involved as cause of this process suggesting that direct interaction with membrane protein thiol groups of the compounds responsible for MPT induction occurs. T. indica extract led to MPTassociated ATP depletion, thus showing the potential to cause cell death by apoptosis or necrosis resulting from MPT induction per se or from ATP depletion by MPT. In vitro, the extract presented free radical scavenging ability, as assessed by the 2,2-diphenyl-1- picrylhydrazyl (DPPH), superoxide radicals and radical hydroxyl assays, and led to decreased lipid peroxidation in mitochondria, as assessed by the thiobarbituric acid reactive substances (TBARS) assay. In addition, the extract showed an iron chelanting property in low concentrations.

nÃo hà / ConstipaÃÃo à um problema clÃnico comum que compreende uma constelaÃÃo de sintomas como excessivo esforÃo, fezes duras, sensaÃÃo de evacuaÃÃo incompleta, uso de manobras digitais, ou defecaÃÃo infreqÃente. Seu tratamento emprega medicamentos que aumentem a freqÃÃncia e facilitem as evacuaÃÃes, bem como mudanÃas nos hÃbitos de vida. O objetivo desse estudo foi avaliar seguranÃa, eficÃcia terapÃutica e validar um mÃtodo analÃtico para a gelÃia TamarineÂ, um fitoterÃpico largamente utilizado como laxante, em pacientes com constipaÃÃo intestinal funcional. Foi realizado o perfil cromatogrÃfico da gelÃia Tamarine para confirmaÃÃo dos seus constituintes e anÃlise de estabilidade empregando cromatografia lÃquida de alta eficiÃncia (CLAE), onde seus principais constituintes foram identificados atravÃs dos tempos de retenÃÃo caracterÃsticos. A anÃlise de estabilidade da formulaÃÃo foi executada comparando os cromatogramas de lotes recÃm-fabricados com lotes apÃs 2 anos de fabricaÃÃo. O mÃtodo desenvolvido comprovou a qualidade das amostras testadas e sua estabilidade apÃs 2 anos de armazenamento. O estudo de toxicologia clÃnica foi do tipo aberto, realizado em 24 voluntÃrios saudÃveis de ambos os sexos que receberam diariamente a administraÃÃo de 5g da gelÃia Tamarine por 28 dias consecutivos. Os voluntÃrios foram avaliados antes, durante e ao final do perÃodo de administraÃÃo atravÃs de histÃria mÃdica, exame fÃsicos e laboratoriais. A formulaÃÃo foi bem tolerada e foram observados feitos adversos como cÃlicas (83,3%), aumento de evacuaÃÃes (58,3%), diarrÃia (20,8%), flatulÃncia e nÃuseas (12,5%). Os parÃmetros hematolÃgicos, metabÃlicos, funÃÃo renal e hepÃtica sofreram variaÃÃes pontuais ao longo do estudo, entretanto, consideradas sem significado clÃnico. O estudo de eficÃcia terapÃutica foi do tipo controlado, paralelo, aberto e randomizado empregando 124 voluntÃrios com constipaÃÃo intestinal funcional, selecionados de acordo com os critÃrios de Roma II e distribuÃdos aleatoriamente entre os grupos de tratamento com a gelÃia Tamarine (TAM) ou Metamucil (MET). Os pacientes em tratamento eram avaliados quinzenalmente para levantamento de efeitos adversos e avaliaÃÃo da eficÃcia feita pelo registro das evacuaÃÃes diÃrias, consistÃncia das fezes e pela melhora global referida pelos mesmos. A maioria dos pacientes apresentou dieta pobre em fibras e baixa ingestÃo de lÃquidos, entretanto, suas dietas nÃo foram alteradas durante o tratamento, as orientaÃÃes nutricionais foram realizadas ao final do estudo para nÃo interferir nos resultados. O uso do TAM aumentou o nÃmero de evacuaÃÃes nos pacientes apÃs 14 e 28 dias de tratamento, quando comparado com o perÃodo inicial de seleÃÃo. No 7o, 14 o, 21 o e 28 o dias a resposta dos pacientes que faziam seu uso foi maior do que o grupo tratado com MET (p&#61500;0,001), bem como a taxa de melhora 166,7% (p=0,003). A consistÃncia das fezes tambÃm melhorou, tornando-se mais amolecidas nos dois grupos. Tratamento com TAM reduziu a consistÃncia das fezes em todo o perÃodo de tratamento (p<0,001) enquanto que o controle teve reduÃÃo apenas na terceira semana (p<0,05). A avaliaÃÃo global dos pacientes (p=0,01 e 0,0367) e do investigador (p=0,0083 e 0,0193) realizadas no 14 e 28 dias de tratamento mostrou melhor aceitaÃÃo para o grupo tratado com TAM nos dois perÃodos de avaliaÃÃo. Nenhum sinal de toxicidade clÃnica ou laboratorial foi observado em todo o perÃodo de tratamento. O presente ensaio clÃnico conclui que a administraÃÃo de 5g da gelÃia Tamarine à segura e eficaz no tratamento da constipaÃÃo intestinal. / Constipation is a common clinical problem that comprises a constellation of symptoms that include excessive straining, hard stools, feeling of incomplete evacuation, use of digital maneuvers, or infrequent defecation. The treatment is composed by behavioral measures and a therapeutic approach. Initial intervention should include dietary measures and fiber supplements; however, if fiber supplement action is ineffective, substances that increase the fecal bolus, lubricants, osmotic agents and laxatives can be used. The aim of the present study was to establish the efficacy, safety and validate an analytical method. Tamarine jelly, a phytotherapic product from Barrenne IndÃstria FarmacÃutica Ltda. a phytomedicine used as laxative. The research project with the experimental protocol and informed consent, were submitted to and approved by the Research Ethics Committee of UFC. The chromatographic profiles of Tamarine jelly was done for confirmation of their constituents and stability analysis using high performance liquid chromatography (HPLC). The method showed the quality of the samples tested and its stability after 2 years of storage. The toxicology clinical trial consisted of an open study with 24 healthy volunteers of both sexes, with a mean age of 26.8 &#61617;&#61472;7.8 years, who orally received a daily dose of 5g Tamarine for 28 uninterrupted days. The volunteers were included in the study only when considered healthy after clinical evaluation, physical examination and laboratory tests which preceded the study. The laboratory tests included: heamatologic, biochemical and serologic analysis. This evaluation was repeated after the first, second, third and fourth week of treatment and at post-study. The jelly was well tolerated, however, some adverse gastrointestinal events were observed. Twenty volunteers (83.3%) reported episodes of light intestinal colic followed by hyperdefecation, five (20.8%) reported diarrhea and three (12.5%) flatulence and nausea. The clinical, electrocardiographic and laboratory tests did not show evidence of toxicity in the various organs and systems evaluated. The efficacy was evaluated in 124 constipated patients selected by Rome II criteria. The investigation was designed as a controlled, parallel and opened. After the selection period, Tamarine (TAM) or Metamucil (MET) was administered. Patients were provided with diary sheets to record each bowel movement and associated subjective symptoms rating stool consistency, abdominal pain and flatus. The efficacy and safety analyzed after each 14 days of treatment by the information recorded on diary and a global assessment of efficacy. Volunteers continuing with their routine activities and maintaining their normal general diets even showing a low consumption of fiber and liquids. The nutritional guidelines were implemented at the end of the study not to interfere in the results. TAM increased bowel frequency after 14 and 28 days when compared with no treatment period and on 7o, 14 o, 21o and 28o days was higher the MET (p<0,001). The stool consistency was changed and became softer in both groups. The alterations improved by TAM was observed in all treatment period (p<0,001) and MET changed only on the third week (p<0,05) . The patientâs global efficacy score (p=0,01 e 0,0367) and investigatorâs (p=0,0083 e 0,0193) realized that on 14 and 28 days of treatment was superior for the TAM group compared with MET. No signs of clinical or laboratory toxicity was observed throughout the treatment period. Confirming their traditional use, the administration of 5g of jelly Tamarine  phytotherapic proved to be safe and effective in the treatment of constipation.

Tamarindus indica L. é um componente natural amplamente consumido por humanos, apresentando propriedade antiinflamatória, antidiabética e antihepatotóxica. Além disso, como nós demonstramos previamente, o extrato apresenta atividade hipolipêmica e antioxidante. Neste trabalho, nós demonstramos o efeito do extrato de T. indica sobre mitocôndrias isoladas de fígado de rato. Na presença de Ca2+, o extrato causou um inchamento osmótico mitocondrial concentração-dependente, associado ao aumento da velocidade basal (V4), dissipação do potencial de membrana e liberação do Ca2+ pré-acumulado, os quais foram inibidos por ciclosporina A (CsA) e assim atribuídos a transição de permeabilidade da membrana mitocondrial (TPMM). A indução do inchamento osmótico mitocondrial foi prevenida por EGTA e vermelho de rutênio, indicando a dependência da TPMM de Ca2+. A oxidação de proteínas tiol mitocondrial, um mecanismo bem estabelecido como causador da TPMM, foi detectado. Entretanto, nenhuma alteração significante foi observada no estado redox de GSH. A oxidação de NAD(P)H e o acúmulo de espécies reativas de oxigênio produzidas pela mitocôndria foram observadas, em ambos os casos, foram prevenidas por CsA e/ou EGTA, indicando que são conseqüências da TPMM induzida pelo extrato de T. indica. Portanto, sem aparente envolvimento do estresse oxidativo no processo, sugerindo uma interação direta de compostos do extrato com grupos tiólicos de proteínas da membrana. O extrato de T. indica induziu uma depleção de ATP associada à TPMM, mostrando assim potencial para causar a morte celular por apoptose ou necrose, resultante da indução da TPMM por si ou pela depleção do ATP via TPMM. In vitro, o extrato apresentou capacidade scavenger de radicais livres, verificado pelo ensaio do DPPH, radical superóxido e radical hidroxil; e ainda foi capaz de diminuir a lipoperoxidação mitocondrial. Além disso, em baixas concentrações, o extrato mostrou uma propriedade quelante de Fe2+. / Tamarindus indica L. is a natural dietary component widely consumed by humans, presenting well established anti-inflammatory, anti-diabetic and anti-hepatotoxic properties. In addition, as we have previously demonstrated, extract presents hypolipemic and antioxidant activities. We show here the effects of T. indica extract on isolated rat liver mitochondria. In the presence of Ca2+, the extract caused mitochondrial concentration-dependent swelling, associated to, resting respiration increase (V4), membrane potential dissipation and release of pre-accumulated Ca2+, inhibited by cyclosporine A (CsA) and thus ascribable to mitochondrial permeability transition (MPT). This swelling induction was prevented by EGTA and ruthenium red, indicating strict dependence of MPT on Ca2+. Oxidation of mitochondrial membrane protein thiols, a well established mechanism causing MPT was detected. However, no significant change was observed in the GSH redox state, and the NADPH oxidation and accumulation of mitochondria-generated reactive oxygen species that was observed, were prevented by CsA and/or EGTA, indicating that they are consequence of the MPT induced by T. indica extract. Therefore, no apparent oxidative stress condition is involved as cause of this process suggesting that direct interaction with membrane protein thiol groups of the compounds responsible for MPT induction occurs. T. indica extract led to MPTassociated ATP depletion, thus showing the potential to cause cell death by apoptosis or necrosis resulting from MPT induction per se or from ATP depletion by MPT. In vitro, the extract presented free radical scavenging ability, as assessed by the 2,2-diphenyl-1- picrylhydrazyl (DPPH), superoxide radicals and radical hydroxyl assays, and led to decreased lipid peroxidation in mitochondria, as assessed by the thiobarbituric acid reactive substances (TBARS) assay. In addition, the extract showed an iron chelanting property in low concentrations.

Der Baobab (Adansonia digitata) ist ein mächtiger und laubabwerfender Baum, der im Sudan heimisch ist. Neben den Früchten bietet er eine Vielzahl von Verwendungsmöglichkeiten u.a. als Nahrungs- und Heilmittel. Die Tamarinde (Tamarindus indica) ist ein graziöser und immergrüner Baum, der im Sudan verbreitet ist. Die Früchte sind bei der Bevölkerung sehr begehrt. Weiterhin ist er Grundlage für viele Forstprodukte. Im Zentralsudan konnten weitere Wildobstarten identifiziert werden, die zur Sicherung der Nahrungsgrundlage der ländlichen Bevölkerung in den Savannen beitragen. Vor allem im Nordsudan existieren große, potenzielle Agrarflächen, auf denen die Bodenversalzung als limitierender Faktor wirkt. Um Nahrungssicherheit für die zunehmende Bevölkerung zu gewährleisten, erlagen diese marginalen Flächen immer mehr an Bedeutung. Die Gewächshausversuche über einen Zeitraum von 20 Wochen zeigten, dass bei Adansonia digitata-Sämlingen das Wachstum und der Stoffwechsel bereits bei Salzbelastungen von 20 mM NaCl stark beeinflusst wurden. Daher kann diese Art im Sämlingsstadium als sehr salzempfindlich eingestuft werden. Tamarindus indica-Sämlinge tolerierten Salzbelastungen von 40 mM NaCl. Daraus folgt, dass die Tamarinde im Vergleich zu anderen Obstarten im Sämlingsstadium relativ salzverträglich ist. Unter den gegebenen Versuchsumständen ist zu vermuten, dass die Tamarinde eher geeignet scheint für die Kultivierung in salzbelasteten semiariden Gebieten. / The Baobab (Adansonia digitata) is a massive, deciduous tree and native to Sudan. Beside its fruits, the Baobab has an exceedingly wide range of uses ranging from food and beverages to medicinal uses. The tamarind (Tamarindus indica) is an aesthetic, evergreen tree and grows wild throughout Sudan. Its fruits are highly appreciated by the people. In addition, the tree provides a diversity of further important forest products. A number of under-utilised species with edible fruits were identified in Central Sudan. Fruits of these indigenous species play an important role in the diet of rural people in the savanna belt, especially during famines and food shortages. Particularly in Northern Sudan there are vast areas of potentially arable land which are affected by salinity to variable degrees. In order to maintain food security for the increasing population, this land is becoming more and more important. The results of the greenhouse experiments over a period of 20 weeks indicate that a salinity level of 20 mM NaCl strongly influences growth and metabolism of Adansonia digitata seedlings. Therefore in the seedling stage the species can be characterised as salt-sensitive. Tamarindus indica seedlings tolerated a salinity level of 40 mM NaCl. This indicates that in the seedling stage tamarind is a fairly salt tolerant tree species compared to other fruit trees. The conclusion of the greenhouse experiments indicates that the tamarind seems to be the more suitable under-utilized fruit tree species for cultivation in semi-arid areas with salinity problems.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-11-06T20:54:56Z No. of bitstreams: 1 IzaelDeSousaCosta_DISSERT.pdf: 1172471 bytes, checksum: 10b2fd67da0043eb1fc836fa80793180 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-11-20T22:08:56Z (GMT) No. of bitstreams: 1 IzaelDeSousaCosta_DISSERT.pdf: 1172471 bytes, checksum: 10b2fd67da0043eb1fc836fa80793180 (MD5) / Made available in DSpace on 2017-11-20T22:08:56Z (GMT). No. of bitstreams: 1 IzaelDeSousaCosta_DISSERT.pdf: 1172471 bytes, checksum: 10b2fd67da0043eb1fc836fa80793180 (MD5) Previous issue date: 2017-08-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A obesidade ? uma epidemia mundial que pode ocorrer por fatores gen?ticos, ambientais e metab?licos / hormonais. Entre os fatores hormonais, sabe-se que, em humanos ou animais, em estado de obesidade, esses apresentam respostas alteradas a determinados horm?nios quando comparados aos eutr?ficos. Por ser uma condi??o de causas multifatoriais, seu tratamento, ocorre por diversas maneiras; entre elas, vem sendo estudada a utiliza??o de inibidores de tripsina. Neste estudo foi avaliado o efeito do ITT como secretagogo de CCK e seu sinergismo sobre leptina em ratos Wistar com obesidade. Inicialmente, ratos Wistar foram classificados quando ao estado nutricional zoom?trico. O estudo foi conduzido com quatro grupos de ratos Wistar, divididos em tr?s grupos de animais com obesidade e um grupo com animais eutr?ficos (n total=20), sendo utilizadas as dietas comercial padr?o (Labina?) e experimental de alto ?ndice glic?mico e alta carga glic?mica (Dieta HGLI), a mesma utilizada para indu??o da obesidade. O ITT (25 mg / Kg de peso) e a Labina? foram utilizadas como tratamentos para dois dos grupos de animais com obesidade, durante dez dias. Aos grupos com obesidade foram administradas: Dieta padr?o mais ?gua (Obeso / Dieta padr?o); Dieta HGLI mais ?gua (Obeso / Dieta HGLI); Dieta HGLI mais ITT (Obeso / Dieta HGLI + ITT); enquanto aos animais eutr?ficos foi oferecida a dieta comercial Labina? (Eutr?fico / Dieta padr?o). Foram verificadas, ao longo desse per?odo, o efeito do ITT sobre o estado nutricional zoom?trico, as concentra??es plasm?ticas de CCK e leptina e a express?o de genes relacionados a CCK em intestino e do gene ob da leptina em gordura subcut?nea. Observou-se que todos os grupos mantiveram seus estados nutricionais de acordo com o ?ndice de Lee por?m os ratos que passaram pelo tratamento com o ITT foram os ?nicos a apresentar uma discreta redu??o desse ?ndice mas, sem signific?ncia estat?stica. O ITT n?o alterou a CCK plasm?tica, n?o tendo sido observadas diferen?as estat?sticas entre os valores de express?o relativa de mRNA para o receptor de CCK-2R e a enzima PCSK1. O tratamento com o ITT aumentou a express?o relativa de mRNA de CCK-1R, assim como ocorreu no grupo sem tratamento que consumia a dieta HGLI, no entanto foi diferente, estatisticamente, dos animais eutr?ficos (p = 0.028). Ademais, foi observado que 60% dos animais, tratados com o ITT, apresentaram concentra??es plasm?ticas de leptina abaixo de 0,1 ng/?L, enquanto aqueles tratados com dieta padr?o, apenas 20% se encontravam nessa condi??o. Tal fato n?o foi observado no grupo eutr?fico e com obesidade. Ainda, relacionado ao efeito do ITT, esse n?o foi capaz de reduzir a express?o relativa de mRNA do gene ob da leptina. O estudo com a express?o relativa de mRNA de genes relacionados ? produ??o de CCK em intestino de ratos com obesidade ? inovador e sugere-se que, ao constatar o efeito do ITT na redu??o das concentra??es plasm?ticas de leptina, neste estudo, o ITT melhore a a??o da CCK em animais com obesidade resultando em discreta melhora do estado de obesidade, independente do aumento plasm?tico da CCK. / Obesity is a worldwide epidemic that can occur due to genetic, environmental and metabolic / hormonal factors. Among the hormonal factors, it is seen that, humans or animals, in state of obesity, present altered responses to certain hormones when compared to eutrophic. Once obesity presents multifactorial causes, its treatment also occurs by several means, among which the use of trypsin inhibitors. This study evaluated the effect of a Tamarindus indica L. seed isolated Trypsin Inhibitor (TTI) as a CCK secretagogue and its synergism with leptin in obese Wistar rats. The study was conducted with obese and lean rats (n = 20). Three groups of obese rats were feed 10 days one of the following diets: Standard diet (Labina?) + water; High Glycemic Index and Load (HGLI) diet + water or HGLI diet + TTI. Lean animals were fed the Labina? for the 10 days. We evaluated nutritional status (zoometric measurements), plasma CCK and leptin, mRNA expression of intestinal CCK-related genes and the ob gene in subcutaneous adipose tissue. TTI treated animals had a slight reduction in the Lee index. TTI did not increase plasma CCK in obese animals and we found no significant effect of TTI in CCK-2R and PCSK1 gene expression. On the other hand, TTI treatment increased CCK-1R gene expression compared with the lean group (p = 0.028). Although TTI did not reduce the ob gene expression. Interestingly 60% of the TTI treated animals presented plasma concentrations below 0.1 ng / ?L, whereas those treated with standard diet, only 20% of the animals were in this condition. This was not observed in the lean and obese groups. Our study with relative mRNA expression of intestinal CCK-related genes in obese rats is a novel study and suggests that TTI by decreasing plasma leptin may improve CCK action in obese rats, since the expression of the CCK-1R gene in this group was higher when compared to lean rats, resulting in improvement of zoometric measurements, independently of plasma CCK increase.

This study expanded the knowledge on tamarind (Tamarindus indica L.) seedling production by analyzing the effects of different container sizes and vermiculite doses in a commercial substrate and seed size and different sowing depths in a nursery seedling production. The experiments were done at the experimental farm Água Limpa in the sector of Fruit Crops, in a nursery with anti-aphid screens, with 0.38 mm2 opening. The farm is located at 19º05 23 S and 48º21 28 W, at 792 m altitude, in the county of Uberlândia, Minas Gerais, Brazil. The experimental design of the trials were randomized blocks, in a 3 x 3 or 2 x 3 factorial. In the first trial, were utilizadas three vermiculite doses (0%, 10% or 20% v v-¹) in three container sizes (18 x 30 cm, 20 x 25 cm or 25 x 35 cm), and in the second trial, utilizou-se two seed sizes (small and large) and three sowing depths (1cm; 2cm or 3cm), both of them with 5 repetitions and 5 plants per plot. The experiments were done from December 26 2006 to June 4 2007. In the first trial, results indicated that tamarind seedlings were not affected by container size, nor by the use of vermiculite. However, the largest container (25 x 35 cm) yielded longer root length, although reducing stem diameter and above ground fresh and dry matter. Mixing vermiculite to the substrate yielded better results for stem diameter, number of leaves, fresh and dry matter of roots and above ground mass. In the second trial, the results indicated that bigger seeds yielded greater quality tamarind seedlings for plant height, stem diameter, fresh and dry matter for above ground mass and roots. There were no significant differences in the parameters evaluated as a function of sowing depth. CHAPTER I: This study evaluated the effects of different vermiculite doses and container sizes in the development and quality of tamarind seedlings. The experiment was done in a nursery with anti-aphid screens, with 0.38 mm2 opening, in the sector of fruit Experimental Farm Água Limpa, county of Uberlândia (MG), from December 26 2006 to June 4 2007. The experimental design was randomized blocks , in a 3 x 3 factorial, with 5 repetitions and 5 plants per plot. The factors were three vermiculite doses (0%, 10% or 20% v v-¹) and three container sizes (18 x 30 cm, 20 x 25 cm or 25 x 35 cm). The development of tamarind seedlings was evaluated by the determination of the number of leaves, stem diameter, plant height, root length, fresh and dry weight of roots and above ground matter. The results showed that tamarind seedling height was not affected by container sized nor by the use of vermiculite. The largest container (25 x 35 cm) yielded better root length; however, stem diameter and above ground fresh and dry matter were reduced. Mixing vermiculite to the substrate yielded better results for stem diameter, number of leaves, fresh and dry matter of roots and above ground mass. CHAPTER II: The effect of seed size and different sowing depths on tamarind (Tamarindus indica L.) seedling vigor and vegetative growth was evaluated in a trial done in a screened nursery, with 0.38 mm2 opening, at the experimental farm Água Limpa, in a nursery with anti-aphid screens, in the county of Uberlândia,. The farm is located at 18º55 33 S and 48º17 19 W, at 872 m altitude, Minas Gerais, from December 26 2006 to June 4 2007. The experimental design was randomized blocks, in a 2 x 3 factorial, with 5 repetitions and 5 plants per plot. The factors were two seed sizes, small and large (65.60 g and 94.54 g respectively), defined according to the weight of 100 seeds and three sowing depths (1cm; 2cm or 3cm). The number of leaves, stem diameter, plant height, root length, fresh and dry weight of roots and above ground matter were evaluated. The results indicate that tamarind seed size affected seedling initial development. There were no significant differences in the parameters evaluated as a function of sowing depth. / O trabalho teve como objetivo ampliar os conhecimentos na produção de mudas de tamarindeiro (Tamarindus indica L.) estudando os efeitos de diferentes tamanhos de recipientes e doses de vermiculita em substrato comercial e tamanho de semente em diferentes profundidades de semeadura na produção de mudas durante a fase de viveiro. Os experimentos foram conduzidos na Fazenda Experimental Água Limpa no setor de Fruticultura, em um viveiro com telado anti-afídios, malha de 0,38 mm2. A Fazenda está situada a 19º05 23 de latitude sul e 48º21 28 de longitude oeste, e altitude de aproximadamente 792 m, no município de Uberlândia-MG. Os delineamentos experimentais foram inteiramente casualizados, com os tratamentos distribuídos em esquemas fatoriais 3 x 3 e 2 x 3. No primeiro experimento, foram utilizadas três doses de vermiculita (0%, 10% e 20% vv ¹) em três tamanhos de recipiente (18 x 30 cm, 20 x 25 cm e 25 x 35 cm), e no segundo experimento, utilizou-se dois tamanhos de semente (pequenas e grandes) e três profundidades de semeio (1; 2 e 3cm), ambos com 5 repetições e 5 mudas por parcela. Os experimentos foram realizados no período de 26 de dezembro de 2006 a 4 de junho de 2007. No primeiro experimento, os resultados mostraram que a altura das mudas de tamarindeiro não foi influenciada pelos diferentes tamanhos de recipiente e uso de vermiculita. No entanto, o maior recipiente (25 x 35 cm) propiciou melhor comprimento de raiz. Todavia, diminuiu o diâmetro de caule e a massa seca da parte aérea das mudas de tamarindeiro. Já a mistura de vermiculita ao substrato promoveu melhores resultados quanto ao diâmetro de caule, número de folhas, massa seca da parte aérea e de raízes. No segundo experimento, os resultados mostraram que as sementes grandes propiciaram mudas de tamarindeiro de qualidade superior, em relação à altura de muda, diâmetro de caule, massa seca, tanto do sistema radicular, como da parte aérea. Quanto à profundidade de semeio, não houve diferença significativa para todas as características avaliadas. CAPITULO I: O objetivo do presente experimento foi avaliar o efeito de diferentes doses de vermiculita e o tamanho dos recipientes no desenvolvimento e na qualidade de mudas de tamarindeiro. O experimento foi conduzido em um viveiro com tela anti-afídios, malha de 0,38 mm2, no setor de Fruticultura da Fazenda Experimental Água Limpa, município de Uberlândia (MG), no período de 26 de dezembro de 2006 a 4 de junho de 2007. O delineamento experimental foi inteiramente casualizado, com os tratamentos distribuídos em esquema fatorial 3 x 3, com 5 repetições e 5 mudas por parcela, sendo os fatores três doses de vermiculita ( 0%, 10% e 20% vv ¹) e três tamanhos de recipiente (18 x 30 cm, 20 x 25 cm e 25 x 35 cm). Para a avaliação do desenvolvimento das mudas de tamarindeiro, foi determinado o número de folhas, diâmetro do caule, altura da muda, comprimento da raiz, peso da matéria seca da parte aérea e das raízes. Os resultados mostraram que a altura das mudas de tamarindeiro não foi influenciada pelos diferentes tamanhos de recipiente e uso de vermiculita. O maior recipiente (25 x 35 cm) propiciou melhor comprimento de raiz, todavia, diminuiu o diâmetro de caule e a massa seca da parte aérea das mudas de tamarindeiro. Já a mistura de vermiculita ao substrato promoveu melhores resultados quanto ao diâmetro de caule, número de folhas, massa seca da parte aérea e de raízes. CAPITULO II: Com o objetivo de avaliar a influência dos tamanhos de semente e a profundidade de semeadura sobre o vigor e o desenvolvimento vegetativo das mudas de tamarindeiro (Tamarindus indica L.), conduziu-se um experimento em um viveiro telado, de malha 0,38 mm2, na Fazenda Experimental Água Limpa, no setor de Fruticultura, município de Uberlândia (MG), no período de 26 de dezembro de 2006 a 4 de junho de 2007. O delineamento experimental foi inteiramente casualizado, com os tratamentos distribuídos em esquema fatorial 2 x 3, com 5 repetições e 5 mudas por parcela, sendo os fatores: dois tamanhos de semente, pequenas e grandes (65,60 g/100 sementes; 94,54 g/100 sementes) respectivamente, e três profundidades de semeio (1; 2 e 3cm). Avaliou-se o número de folhas, diâmetro do caule, altura da muda, comprimento da raiz, massas secas da parte aérea e das raízes. Os resultados obtidos mostraram que as sementes de tamarindo influenciaram o desenvolvimento inicial das mudas. Quanto à profundidade de semeio, não houve diferença significativa entre todas as características avaliadas nas mudas. / Mestre em Agronomia

Submitted by Johnny Rodrigues (johnnyrodrigues@ufcg.edu.br) on 2018-05-03T18:10:31Z No. of bitstreams: 1 WANDRA LAURENTINO DA SILVA - DISSERTAÇÃO PPGSA ACADÊMICO 2017.pdf: 1274770 bytes, checksum: 86d0bf49883135653ae9e8e952db2a0d (MD5) / Made available in DSpace on 2018-05-03T18:10:31Z (GMT). No. of bitstreams: 1 WANDRA LAURENTINO DA SILVA - DISSERTAÇÃO PPGSA ACADÊMICO 2017.pdf: 1274770 bytes, checksum: 86d0bf49883135653ae9e8e952db2a0d (MD5) Previous issue date: 2017-08-17 / O nordeste apresenta deficit hídrico e precipitações pluviométricas irregulares, além de altas temperaturas, exigindo para a exploração agrícola racional, o uso de irrigação. A reduzida disponibilidade no semiárido, de água de boa qualidade para irrigação, muitas vezes, torna necessário o uso de águas salinas, ficando esse uso na dependência de desenvolvimento de técnicas que tornem possível a exploração agrícola sustentável ou seja, sem agredir o meio ambiente.Com isso, objetivou-se com está pesquisa avaliar a produção de mudas de tamarindo cultivar Crioula irrigadas com águas salinizadas ob adubação nitrogenada. O experimento foi desenvolvido em ambiente protegido (casa de vegetação) no Centro de Ciências e Tecnologia Agroalimentar da Universidade Federal de Campina GrandePombal-PB. O delineamento experimental utilizado foi em blocos casualizados, num esquema fatorial 5 x 4, com quatro repetições e duas plantas por parcela. Os tratamentos consataram de cinco níveis de condutividade elétrica da água de irrigação (0,3; 1,3; 2,3; 3,3 e 4,3 dS m-1) em quatro doses nitrogenio (70, 100, 130 e 160%).A dose de 100% corresponde á 600 mg de nitrogênio dm3. mudas de tamarindo). A produção das mudas foi avaliada através das distintas variáveis de crescimento: altura de planta, diâmetro do caule e número de folhas no período de 90 dias após a aplicação dos tratamentos (DAT)e as variáveis de fitomassa fresca e seca de caule, folhas, parte aérea, raiz, e seca total, no período de 135(DAT). Na produção de mudas de tamarindo pode-se usar água de condutividade elétrica de 1,7dS m-1 pois proporciona redução media aceitável de 10% no crescimento. Doses de N variando de 70 a 160 mg de N dm-3 de solo assim como, a interação entre os fatores estudados não promoveram efeito sobre as variáveis estudadas. / The northeast presents water deficit and irregular rainfall, besides high temperatures, requiring for rational agricultural exploitation, the use of irrigation.The low availability in the semi-arid region of good quality water for irrigation often necessitates the use of salt water, and this use is dependent on the development of techniques that make sustainable agricultural exploitation possible, that is, without harming the environment.The objective of this research was to evaluate the production of tamarind seedlings cultivated with Criolla irrigated with salinized waters under nitrogen fertilization.The experiment was carried out in a protected environment (greenhouse) at the Agro-Food Science and Technology Center of the Federal University of Campina Grande Pombal-PB.The experimental design was a randomized complete block design in a 5 x 4 factorial scheme with four replications and two plants per plot. The treatments consisted of five levels of electrical conductivity of irrigation water (0.3, 1.3, 2.3, 3.3 and 4.3 dS m-1) in interaction with four nitrogen doses (70, 100, 130 And 160% The dose of 100% corresponds to 600 mg of nitrogen dm-3 tamarind seedlings).The seedlings production was evaluated through the different growth variables: plant height, stem diameter and number of leaves in the period of 90 days after the application of the treatments (DAT) and the variables of fresh and dry phytomass of leaves, leaves , Aerial part, root, and total dry, in the period of 135 (DAT).In the production of tamarind seedlings, water of electrical conductivity of 1.7 dS m-1 can be used as it provides an acceptable average reduction of 10% in growth. Doses of N varying from 70 to 160 mg of N dm-3 of soil as well, the interaction between the studied factors did not have an effect on the variables studied.

Le taro (Colocasia esculenta L SCHOTT) est un tubercule d’une grande importance alimentaire au Tchad pour la consommation humaine et animale. La littérature sur les pratiques culturales et les technologies traditionnelles post-récolte des variétés tchadiennes demeure presque inexistante. Une enquête a donc été menée dans la région du Mayo-Kebbi (Tchad) où le taro est très cultivé. Il résulte qu’il existe principalement deux variétés de taro au Tchad : la variété « Gouning souol» ou variété locale qui est caractérisée par une âcreté importante et un temps de cuisson prolongé (6 à 8 h). Ces propriétés ont entraîné l’abandon de la culture de cette variété au profit de la variété « Gouning sosso » qui présente une âcreté moindre et un temps de cuisson plus court (45 à 60 minutes). Une technique traditionnelle pour réduire l’âcreté et diminuer le temps de cuisson consiste à tremper les tranches de taro frais soit dans l’eau, soit dans une solution de trempage de maïs ou soit dans une infusion de tamarin pendant 24 à 48 h selon l'intensité de l'âcreté. Après séchage au soleil, les cossettes sèches sont transformées en farine. Ces produits de transformation servent à la préparation de boules de pâtes, de bouillie, de soupes ou de beignets. Des analyses ont été effectuées sur une gamme d’échantillon de farine de taro produite conformément à la recette artisanale dans l’optique de comprendre l’effet de cette technologie traditionnelle. Des cossettes fraîches de taro ont été trempées pendant 0, 1 h, 3 h, 6 h, 12 h et 24 h dans de l’eau, ou dans une solution de trempage de maïs ou dans une infusion de tamarin. Chaque échantillon a ensuite été séché au soleil pendant 48 h puis broyé en farine. La matière sèche, les protéines brutes, les cendres, les fibres brutes, les macroéléments minéraux et oligoéléments ont été évalués dans le but de déterminer les effets du trempage traditionnel sur le profil nutritionnel du taro. La digestibilité in vitro de l’amidon sous l’action de l’α-amylase et la teneur en oxalates de différentes farines de taro ont été également déterminées. Il ressort de ces analyses que seul le trempage dans une infusion de tamarin a induit une baisse significative (P < 0,001) de la teneur en protéines brutes (de 3,26 % pour T0 à 2,68 % pour le traitement au tamarin à T24). L’infusion de tamarin n’a pas modifié la teneur en fibres brutes alors que les autres traitements l’ont assez significativement diminuée (P < 0,001). Toutes les méthodes de trempage ont entraîné une diminution significative des teneurs en minéraux, à l’exception d’une augmentation des teneurs en sodium (due à la qualité de l’eau des préparations) et en phosphore (probablement due à l’hydrolyse des phytates du maïs). Une perte significative en fer a été constatée passant de 144 mg/kg de MS (témoin) à 78 mg/kg de MS (échantillon traité à l’eau pendant 12 h), soit une perte de 45,83 %. La plus faible diminution de taux de fer est observée dans le cas de l'échantillon trempé dans l'infusion de tamarin avec une perte maximale de 31,25 % après 24 h. La teneur en zinc ne varie pas. Les procédés de trempage entraînent globalement une amélioration de la digestibilité du taro après une simple cuisson (95°C pendant 30 minutes) qui passe de 39,30 % (échantillon témoin non trempé) à 78,67 % pour le taro trempé dans l’eau. Après 3 h de trempage, la farine de taro traitée par la solution de maïs est significativement plus digestible (77,12 %) que les échantillons résultant de deux autres traitements [tamarin (60,86 %), eau (60,45 %)] qui sont statistiquement similaires. (...) / Colocasia esculenta L SCHOTT is a valuable tuber widely consumed in Chad by humans and livestock. There is very little available literature about the cultivation and the post-harvest traditional technologies of the Chadian varieties of taro. A survey was carried out in the Mayo-Kebbi region ( Chad) where taro is a widely cultivated. There are two main varieties of taro in Chad : the " Gouning souol " variety, a local variety characterized by an important level of acridity and the prolonged cooking time (6 to 8 hours). The presence of these properties led to the replacement of the cultivation of this variety by the " Gouning sosso " variety which presents a lower level of acridity and a shorter cooking time (45 to 60 minutes). A traditional process to reduce the acridity and decrease the cooking time consists in dipping the slices of fresh taro in the water, or in a corn solution or in a tamarind infusion for a period of 24 to 48 hours according to the intensity of the acridity. After drying in the sun, the dried chips are ground into flour. This flour is then used to prepare mixtures of porridge, soups and cakes. Analyses were carried out on samples of taro flour produced in accordance with the traditional methods of soaking to determine the effects of this traditional process. Fresh taro chips were dipped over a period of 0, 1, 3, 6, 12 and 24 hours in the water, or in the corn infusion or in the tamarind infusion. Each sample was then dried in the sun for 48 hours and ground into flour. Dry matter, crude protein, ash, crude fiber, minerals and various trace elements were assessed. The in vitro digestibility of taro starch using α-amylase enzyme and the amount of total oxalates in the different taro flour were also determined. The soaking in the tamarind infusion induced a significant (P < 0.001) decrease in protein content (from 3.26 % for the control sample to 2.68 % for the treatment with the tamarind infusion at T24). The other treatments (corn solution and water) reduced also the protein level of taro but not significantly. The crude fibre content was not affected by the tamarind infusion but decreased significantly in the other treatments at the threshold level of 5 %. All methods of soaking led to a significant decrease of the mineral contents, except for an increase in sodium (due to the quality of the water used to prepare solutions) and in phosphorus (probably due to the hydrolysis of phytates by the corn infusion). A significant loss in trace element (Iron) was observed, decreasing from 144 mg / kg of DM (control sample) to 78 mg / kg of DM (after 12 hours of water soaking) corresponding to a loss of 45.83 %. The lowest decrease in iron level was observed after the treatment by the tamarind infusion (31.25 % after 24 hours). All the soaking processes were seen to have improved the digestibility of taro flour. By simply cooking (at a temperature of 95°C for 30 minutes), the in vitro digestibility increased from 39.30 % for the control sample to 78.67 % for the water treated samples of taro. After three hours of soaking, the taro flour treated by the solution of corn becomes significantly more digestible (77.12 %) than by the two other treatments [tamarind (60.86 %), water (60.45 %)] that are statistically similar. (...)

La coiffe racinaire est cruciale à la croissance et survie du méristème subapical de racine. Elle libère des cellules frontières (CFs) qui assurent la protection de l’apex racinaire. Les CFs associées à leur mucilage forment le piège extracellaire de racine (RET). La caractérisation du RET et des exsudats racinaires de trois essences ligneuses sahéliennes à partir de plantules cultivées in vitro a été réalisée. B. aegyptiaca et A. raddiana prospèrent dans les zones semi-arides, à l’opposé de T. indica. La morphologie des CFs et l’organisation du RET ont été déterminées par microscopie. La compostion en glycopolymères et la détection des arabinogalactanes proteines (AGPs) dans le RET et les exsudats racinaires ont été déterminées par des analyses biochimiques. L’effet des exsudats racinaires sur la croissance d’Azospirillum brasilense, une bactérie bénéfique pour la plante a été évalué. B. aegyptiaca produit des CFs de type border cells (BCs) alors que les autres Fabaceae produisent des BCs et des border-like cells. Les BCs sont entourées d’un dense mucilage riche en polymères de paroi. Le RET et les exsudats racinaires issus de B. aegyptiaca et A. raddiana sont plus riches en AGPs que ceux provenant T. indica. Les AGPs pourraient contribuer à la survie des plantules dans un contexte semiaride. Ce travail ouvre de nouvelles perspectives de recherche concernant l'implication du RET dans la survie des plantes à l'aridité. / The root cap is primordial for seedling growth and supports root apical meristem integrity. The root cap releases root border cells (RBCs) that surround the root tip and ensure seedling protection against numerous stresses. RBCs and their associated mucilage form the root extracellular trap (RET). Here, RET and root exudate characterization of three Sahelian woody seedlings are performed. In contrast to B. aegyptiaca and A. raddiana which thrive in semi-arid areas, T. indica is more sensitive to drought. B. aegyptiaca, A. raddiana and T. indica seedlings were sub-cultured in vitro. RBC morphologies and RET organization were determined using microscopic approaches. The polysaccharide composition and arabinogalactan protein (AGP) content were determined by biochemical approaches in the RET and the root exudates. Moreover, the effect of root exudates on the growth of Azospirillum brasilense a plant benefical bacteria has been performed. While B. aegyptiaca produces only border cell (BC) type, the two Fabaceae seedlings release both BCs and border-like cells (BLCs). BCs are enclosed in a dense mucilage enriched in cell wall polymers. Compared to T. indica, RET and root exudates of B. aegyptiaca and A. raddiana include more abundant AGPs. In this context, AGPs could contribute to woody seedling survival. This work opens new research perspectives regarding involvement of RET in plant survival to aridity.