This work deals with the creation of a graphic demo with the use of inverse kinematics. It explains the motivations that led to the creation of the demo; it briefly mentions the history of the demo as well as the principles of animation. It describes in detail the fundamentals of inverse kinematics as well as the terminology needed for the understanding of inverse kinematics. It also includes some selected algorithms. The issue of inverse kinematics is further developed in the part of the work where the principles of skinning are being described. The principles of the collision detection are also mentioned here. The conclusion consists of a detailed explanation of some of the techniques used in graphic demo, such as the L-systems or procedural textures.

安全監控產業在美國遭受911恐怖攻擊世事件後，加上隨後英國、西班牙、印尼峇里島等陸續遭到爆炸恐怖攻擊，造成嚴重的生命財產損失，因而喚起各國政府對安全防護的重視，所以該產業隨之發展迅速，但也相對的吸引了其它相對低毛利的產業競相跨足進入此一新興產業，而使得原本一個不顯眼的產業一下子熱絡了起來，當然競爭的情況也日益加劇。 安全監控未來的發展將會朝向數位化、網路化以及系統整合方向發展，此乃世界潮流之趨勢，其中又以網路攝影機及NVR網路數位式錄影軟體、DVR數位式錄影機等所扮演之角色日趨重要。 如何在這個具有高度競爭的產業脫穎而出，筆者以波特的五力分析模型及邱志聖的4C策略行銷架構剖析H公司所從事之安控產業的競爭現況後，得到的結論認為: 若單以成本優勢為主要策略而不搭配其產品功能效益是不易成功的，廠商應該以長期投入研發、配合降低成本，以市場需求為導向，而突顯出產品價值才有可能勝出。從長期培養客戶關係著手，隨時提供最新產品資訊或者公司未來將要開發的產品，以降低客戶對現在及未來產品的資訊蒐集成本。與上游供應商保持密切合作關係，如IC設計開發公司智原以及與SONY、 PANASONIC等CCD元件供應商持續合作，並提供良好的售後服務，以降低客戶的道德危機成本等。透過軟體的高度整合以及提供良好的客製化服務及產品，使客戶消除專屬陷入成本的疑慮，以上將是H公司對未來發展重要參考策略，或者可為有意加入此行業的新進者做為參考方向。

Designing good digital experiences for children can be difficult; designers have to consider children's cognitive and motor skill limitations, understand their target audience, create something entertaining and educational, comply with national and international jurisdiction, and at the same time appeal to parents. We set out to create a general framework which designers and developers can use as a foundation and testing ground for their digital products in the field of user experience. The methods used during the thesis include interviews, literature studies, user testing, case studies, personas, prototyping, and more. The results created are primarily user experience guidelines packaged in a Theoretical Framework, user testing conclusions, along with suggestions on improving the current Lego Star Wars: Force Builders application, a few in the form of prototypes.

Approved for public release; distribution in unlimited. / Traditionally, digital imaging systems rely on the use of dedicated photodetectors to capture specific wavelengths in the visible spectrum. These photodetectors, which are commonly made of silicon, are arranged as arrays to capture the red, green and blue wavelengths. The signal captured by the individual photodetectors must then be interpolated and integrated to obtain the closest color match and the finest possible resolution with reference to the actual object. The use of spatially separated detectors to sense primary colors reduces the resolution by a factor of three compared to black and white imaging. The FOVEON detector technology greatly improves the color and resolution of the image through its vertically arranged, triple well photodetector. This is achieved by exploiting the variation of absorption coefficient of silicon with wavelength in the visible spectrum. Hence, in a silicon detector, the shorter wavelength (e.g. blue) would be mainly absorbed at a shallow depth. A longer wavelength (e.g. red) would penetrate the material deeper than the shorter wavelengths and be primarily absorbed at a greater depth. By producing a layered silicon detector, all three primary colour wavelengths of red, green and blue can be captured simultaneously. This thesis aims to study the FOVEON camera's ability to image light from the near Infrared (NIR) to the Ultra-Violet (UV) range of the electromagnetic spectrum. The imaged obtained using a set of bandpass filters show that the camera has response both in the UV as well as NIR regions. / Major, Singapore Armed Forces

Il y a presque dix ans que l'accélération de l'expansion de l'Univers a été mise en évidence grâce aux observations des supernovae de type Ia et du fonds diffus cosmologique. Cette découverte a changé notre compréhension du contenu énergétique de l'Univers puisque pour expliquer une telle accélération, une composante supplémentaire de matière (effective ou non) est nécessaire et contribue à hauteur de 70%. Cette dernière est appelé «énergie noire». Elle affecte aussi bien les mesures de distance, que la croissance des sur-densités de matières primordiales qui donnent naissance aux structures. Les principales sondes sensibles à ces deux dernières quantités sont les supernovae de type Ia, les amas de galaxies, les lentilles gravitationnelles, et les oscillations acoustiques des baryons (BAO). Afin de contraindre précisément les modèles théoriques (Constante Cosmologique, modification de la théorie de la relativité générale par exemple) qui tentent de déterminer la nature de l'énergie noire, l'observation de chacune de ces quatre sondes est indispensable. Le niveau de précision sur la mesure des paramètres des modèles d'énergie noire requis est tel qu'une nouvelle génération d'instruments va voir le jour dans les années à venir avec notamment le télescope LSST (Large Synoptic Survey Telescope). Le télescope LSST dont le miroir primaire fait 8.4 mètres de diamètre, produira un sondage couvrant la moitié du ciel observable dans 6 bandes photométriques pendant 10 ans. Sa caméra sera la plus grosse caméra jamais construite dans le monde avec un plan focal de 3.2 milliards de pixels. Cette thèse présente à la fois un aspect expérimental et phénoménologique. Le travail présenté porte tout d'abord sur l'élaboration du banc d'étalonnage de la caméra de LSST, et des premières mesures optiques validant le schéma de principe du banc. Nous présenterons ensuite la simulation des BAO dédiée à LSST tentant de prédire à quelle précision les paramètres d'énergie noire pourront être contraint. L'accent est mis sur la production d'un catalogue photométrique de galaxies simulé ainsi que sur une méthode de calcul des redshifts photométriques. La validation de la méthode grâce à des données spectro-photométriques du CFHTLS est également présentée. / More than ten years ago, the accelerated expansion of the Universe was discovered, by type Ia supernovae, and then confirmed by other probes. This discovery has changed our understanding of the energetic content of the Universe. Indeed, in order to explain such an acceleration, a new component has to be introduced and it must contribute to 70% of the total energy density. This component, the so called Dark Energy, affects both cosmological distances and the growth of structures from which galaxies originates. The main cosmological probes of dark energy are the type Ia supernovae, the galaxy cluster count, the weak gravitational lensing and the baryon acoustic oscillations (BAO). In order to precisely constrain theoretical models, such as the cosmological constant, a modify gravity or a new scalar field, joint observations of all four probes are very efficient. The required accuracy on cosmological measurements is so high that a new generation of instruments is growing, among which the Large Synoptic Survey Telescope (LSST). The telescope, with a primary mirror of 8.4 m diameter, will cover half of the optical sky in six photometric bandpasses. Its camera will be the world biggest camera ever constructed with a focal plane array composed of 3.2 Gpixels. This thesis treats both the experimental and phenomenological aspects. Firstly, the work presented here consists in the development of the LSST camera calibration optical bench. We have designed a system allowing an efficient commissioning of the camera before its installation on the telescope, and a precise calibration of the focal plane. Preliminary measurements validating the design of the bench will be presented. Secondly, a detailed Baryon Acoustic Oscillations simulation dedicated to LSST will be introduced. Its main goal is to predict the level of precision on the dark energy equation of state parameter reconstruction that will be reached with LSST. We will stress on the production of a mock photometric galaxy catalog and on the photometric redshifts computation. A validation of the method on real spectro-photometric from CFHTLS will also be shown.

Neste trabalho avaliou-se a produção de compostos de interesse biotecnológico potenciais vetores energéticos a partir de papel em lisímetros (20L), usando-se consórcio microbiano enriquecido do fluido de rúmen. Para tanto, foi realizado um delineamento composto central (DCC) para verificar a influência de três variáveis independentes na conversão de papel sulfite a hidrogênio e outros compostos orgânicos em lisímetro de bancada. As variáveis testadas foram massa de papel (X1: 500g, 750g e 1000g), teor de umidade papel (X2: 50%, 65% e 80%), e temperatura (X3:35°C, 45°C e 55°C). As respostas avaliadas no DCC foram produção de hidrogênio (Y1; mmol), ácido acético (Y2; mg/L), etanol (Y3; mg/L) e metanol (Y4; mg/L). Para o monitoramento dos lisímetros em relação à hidrólise e fermentação do papel, foram analisados biogás (H2, N2, CO2 e CH4) e a concentração de compostos no percolado, como açúcares totais, demanda química de oxigênio (DQO), ácidos orgânicos voláteis (AOVs) e álcoois. Além disso, monitorou-se o pH, alcalinidade e sólidos totais. Sequenciamento massivo do gene RNAr 16S via Plataforma Illumina foi usado para identificação dos micro-organismos do fluido de rúmen in natura, do consórcio enriquecido, e daqueles dos lisímetros R2, R5 e R9. Produção de hidrogênio só foi observada nos lisímetros R1 (25 mmol), R2 (35 mmol) e R5 (3 mmol), sendo os três com umidade inicial de 80%. Em R1 e R2, observou-se elevadas concentrações de ácido acético, de 21.500 e 17.000 mg/L, respectivamente, provavelmente devido à ocorrência de homoacetogênese. Sob temperatura termofílica, especialmente em R5, observou-se consumo de hidrogênio, e produção de etanol (2.300 mg/L) e metanol (5.600 mg/L). Na condição de 80% de umidade (R1, R2, R5, R6), verificou-se maiores percentuais de remoção de papel e atividade fermentativa mais acentuada, ao passo que abaixo de 80%, o desenvolvimento microbiano foi desfavorecido, independente da temperatura. Verificou-se consumo muito reduzido de papel e baixas concentrações de AOVs e álcoois para R3, R4, R7 e R8, todos com 50% umidade. Em R9 e R10, operados a 45 °C e 65% de umidade, também verificou-se produção atenuada de AOVs e álcoois, com ausência de hidrogênio. Por meio do DCC, observou-se efeito estatisticamente significativo da umidade do papel na produção de hidrogênio, ácido acético e etanol. Em relação à temperatura, verificou-se efeito positivo estatisticamente significativo na produção de hidrogênio e ácido acético. Por fim, para a massa de papel não se verificou nenhum efeito sobre as respostas analisadas. Os gêneros de bactérias mais abundantes foram: Prevotella no fluido de rúmen in natura (F.N.) Dysgonomonas no fluido de rúmen enriquecido e em R2 (35°C), Thermicanus em R5 (55°C) e Phaeospirillum em R9 (45ºC). A umidade foi o parâmetro mais determinante para promover a hidrólise e fermentação do papel; a temperatura foi a principal variável de influência na estrutura das comunidades microbianas dos lisímetros, confirmada pelas diferentes rotas metabólicas observadas sob temperatura mesofílica e termofílica; e os rendimentos de produção dos compostos não foram influenciados pela massa de papel adicionada aos lisímetros. / This study evaluated the production of compounds of biotechnological interest and potential energy vectors from office paper in lysimeters (20L), using a microbial consortium purified from rumen fluid. A central composite design (CCD) was performed to verify the influence of three independent variables on paper conversion to hydrogen and other organic compounds in bench lysimeter. The tested variables were: mass of paper (x1: 500g, 750g and 1000g), moisture content (x2: 50%, 65% and 80%), and incubation temperature (x3: 35°C, 45°C and 55°C). The dependent variables of CCD were production of hydrogen (Y1: mmol), acetic acid (Y2: mg/L), ethanol (Y3:mg/L) and metanol (Y4:mg/L). For monitoring the lysimeters in relation to paper hydrolysis and fermentation, analyses of biogas (H2, N2, CO2 and CH4) and the organic compounds' concentrations in the leachate, such as, total sugars, chemical oxygem demand, volatile organic acids (VOA's) and alcohols were conducted during operation. Alcalinity, pH and total solids content of the leachate were also monitored. Massive sequencing of rRNA 16S (Illumina) was carried out for identification of the microorganisms of the in natura rumen fluid, the purified consortium, and those collected from lysimeters R2, R5 and R9. Hydrogen production was detected only in lysimeters R1 (25 mmol), R2 (35 mmol) and R5 (3 mmol), all of them operated with 80% of moisture content. In R1 and R2, high concentrations of acetic acid, of 21.500 and 17.000, respectively, were due to the likely occurrence of homoacetogenesis. Under thermophilic temperature, especially R5, the hydrogen production was detected in low quantity, and the highlight was the production of ethanol and methanol, with concentrations around 2.300 and 5.600 mg/L, respectively. At 80% moisture condition (R1, R2, R5, R6), high percentages of paper removal and sharp fermentative activity were observed. However, at lower moisture conditions, the microbial growth was unfavored, independent of the temperature. Low paper consumption and reduced concentrations of OVA's and alcohols were detected in R3, R4, R7 and R8, all of them operated with 50% of moisture content. In R9 and R10, operated at 45°C and 65% of moisture, there was also attenuated production of VOA\'s and alcohols, with absence of hydrogen. According to CCD statistical analysis, paper moisture content had positive effect statistically significative on hydrogen, acetic acid and etanol production. The temperature had positive effect on hydrogen and acetic acid production. And the mass of paper dit not have effect statistically significative for any dependent variables. The most abundant bacterial genus was: Prevotella in the in natura rumen fluid (F.N.) Dysgonomonas in the purified consortium and R2 (35 oC), Thermicanus in R5 (55°C) and Phaeospirillum in R9 (45° C). In conclusion, moisture content was the main parameter to promote paper hydrolysis and fermentation; temperature was the principal variable that influenced the structure of microbial community, confirmed by the different metabolic route observed under mesophilic and thermophilic conditions; and the production yields of the compounds were not influenced by the mass of paper added to the lysimeters.

Neste trabalho avaliou-se a produção de compostos de interesse biotecnológico potenciais vetores energéticos a partir de papel em lisímetros (20L), usando-se consórcio microbiano enriquecido do fluido de rúmen. Para tanto, foi realizado um delineamento composto central (DCC) para verificar a influência de três variáveis independentes na conversão de papel sulfite a hidrogênio e outros compostos orgânicos em lisímetro de bancada. As variáveis testadas foram massa de papel (X1: 500g, 750g e 1000g), teor de umidade papel (X2: 50%, 65% e 80%), e temperatura (X3:35°C, 45°C e 55°C). As respostas avaliadas no DCC foram produção de hidrogênio (Y1; mmol), ácido acético (Y2; mg/L), etanol (Y3; mg/L) e metanol (Y4; mg/L). Para o monitoramento dos lisímetros em relação à hidrólise e fermentação do papel, foram analisados biogás (H2, N2, CO2 e CH4) e a concentração de compostos no percolado, como açúcares totais, demanda química de oxigênio (DQO), ácidos orgânicos voláteis (AOVs) e álcoois. Além disso, monitorou-se o pH, alcalinidade e sólidos totais. Sequenciamento massivo do gene RNAr 16S via Plataforma Illumina foi usado para identificação dos micro-organismos do fluido de rúmen in natura, do consórcio enriquecido, e daqueles dos lisímetros R2, R5 e R9. Produção de hidrogênio só foi observada nos lisímetros R1 (25 mmol), R2 (35 mmol) e R5 (3 mmol), sendo os três com umidade inicial de 80%. Em R1 e R2, observou-se elevadas concentrações de ácido acético, de 21.500 e 17.000 mg/L, respectivamente, provavelmente devido à ocorrência de homoacetogênese. Sob temperatura termofílica, especialmente em R5, observou-se consumo de hidrogênio, e produção de etanol (2.300 mg/L) e metanol (5.600 mg/L). Na condição de 80% de umidade (R1, R2, R5, R6), verificou-se maiores percentuais de remoção de papel e atividade fermentativa mais acentuada, ao passo que abaixo de 80%, o desenvolvimento microbiano foi desfavorecido, independente da temperatura. Verificou-se consumo muito reduzido de papel e baixas concentrações de AOVs e álcoois para R3, R4, R7 e R8, todos com 50% umidade. Em R9 e R10, operados a 45 °C e 65% de umidade, também verificou-se produção atenuada de AOVs e álcoois, com ausência de hidrogênio. Por meio do DCC, observou-se efeito estatisticamente significativo da umidade do papel na produção de hidrogênio, ácido acético e etanol. Em relação à temperatura, verificou-se efeito positivo estatisticamente significativo na produção de hidrogênio e ácido acético. Por fim, para a massa de papel não se verificou nenhum efeito sobre as respostas analisadas. Os gêneros de bactérias mais abundantes foram: Prevotella no fluido de rúmen in natura (F.N.) Dysgonomonas no fluido de rúmen enriquecido e em R2 (35°C), Thermicanus em R5 (55°C) e Phaeospirillum em R9 (45ºC). A umidade foi o parâmetro mais determinante para promover a hidrólise e fermentação do papel; a temperatura foi a principal variável de influência na estrutura das comunidades microbianas dos lisímetros, confirmada pelas diferentes rotas metabólicas observadas sob temperatura mesofílica e termofílica; e os rendimentos de produção dos compostos não foram influenciados pela massa de papel adicionada aos lisímetros. / This study evaluated the production of compounds of biotechnological interest and potential energy vectors from office paper in lysimeters (20L), using a microbial consortium purified from rumen fluid. A central composite design (CCD) was performed to verify the influence of three independent variables on paper conversion to hydrogen and other organic compounds in bench lysimeter. The tested variables were: mass of paper (x1: 500g, 750g and 1000g), moisture content (x2: 50%, 65% and 80%), and incubation temperature (x3: 35°C, 45°C and 55°C). The dependent variables of CCD were production of hydrogen (Y1: mmol), acetic acid (Y2: mg/L), ethanol (Y3:mg/L) and metanol (Y4:mg/L). For monitoring the lysimeters in relation to paper hydrolysis and fermentation, analyses of biogas (H2, N2, CO2 and CH4) and the organic compounds' concentrations in the leachate, such as, total sugars, chemical oxygem demand, volatile organic acids (VOA's) and alcohols were conducted during operation. Alcalinity, pH and total solids content of the leachate were also monitored. Massive sequencing of rRNA 16S (Illumina) was carried out for identification of the microorganisms of the in natura rumen fluid, the purified consortium, and those collected from lysimeters R2, R5 and R9. Hydrogen production was detected only in lysimeters R1 (25 mmol), R2 (35 mmol) and R5 (3 mmol), all of them operated with 80% of moisture content. In R1 and R2, high concentrations of acetic acid, of 21.500 and 17.000, respectively, were due to the likely occurrence of homoacetogenesis. Under thermophilic temperature, especially R5, the hydrogen production was detected in low quantity, and the highlight was the production of ethanol and methanol, with concentrations around 2.300 and 5.600 mg/L, respectively. At 80% moisture condition (R1, R2, R5, R6), high percentages of paper removal and sharp fermentative activity were observed. However, at lower moisture conditions, the microbial growth was unfavored, independent of the temperature. Low paper consumption and reduced concentrations of OVA's and alcohols were detected in R3, R4, R7 and R8, all of them operated with 50% of moisture content. In R9 and R10, operated at 45°C and 65% of moisture, there was also attenuated production of VOA\'s and alcohols, with absence of hydrogen. According to CCD statistical analysis, paper moisture content had positive effect statistically significative on hydrogen, acetic acid and etanol production. The temperature had positive effect on hydrogen and acetic acid production. And the mass of paper dit not have effect statistically significative for any dependent variables. The most abundant bacterial genus was: Prevotella in the in natura rumen fluid (F.N.) Dysgonomonas in the purified consortium and R2 (35 oC), Thermicanus in R5 (55°C) and Phaeospirillum in R9 (45° C). In conclusion, moisture content was the main parameter to promote paper hydrolysis and fermentation; temperature was the principal variable that influenced the structure of microbial community, confirmed by the different metabolic route observed under mesophilic and thermophilic conditions; and the production yields of the compounds were not influenced by the mass of paper added to the lysimeters.

Le noyau d'une cellule est hétérogène par sa structure et son activité et beaucoup de ses composants interagissent de façon dynamique. Lors de l'étude de processus cellulaires comme la réponse au stress thermique, des expériences classiques de spectroscopie de corrélation de fluorescence (FCS), qui sont habituellement limitées à un seul volume d'observation, n'apportent que des résultats partiels à cause des informations spatiales manquantes. Ce mémoire de thèse présente une nouvelle technique de FCS multi-confocale (mFCS) qui permet des mesures FCS simultanées à différents endroits d'une cellule. La technique est basée sur l'emploi d'un modulateur spatial de lumière pour la création de plusieurs volumes d'observations distincts et d'une caméra ``electron-multiplying'' CCD (EMCCD) pour la détection en parallèle. La résolution spatiale ainsi que la sensibilité du système mFCS sont proches de celles d'un système FCS classique et en utilisant un mode d'acquisition particulier une résolution temporelle de $14mu s$ a pu être atteinte. La technique mFCS est appliquée à l'étude de la réponse cellulaire au stress thermique en observant le facteur de transcription heat shock factor 1 (HSF1), qui est un régulateur clé de la réponse au stress thermique. Des mesures mFCS dans des cellules vivantes révèlent des changements dans la dynamique de HSF1 pendant le choc thermique. Ces changements concernent l'affinité ainsi que l'homogénéité spatiale des interactions avec l'ADN. En outre, nous avons également évalué les performances d'une caméra CMOS-SPAD et testé le dispositif en tant que capteur alternatif pour la mFCS en cellules vivantes. / The cell nucleus is heterogeneous in its structure and activity and many of its components are in dynamic interactions with each other. When investigating the cellular response to an external signal, such as heat shock, standard fluorescence correlation spectroscopy (FCS) experiments, which are limited to one observation volume, do only give partial results because of the missing spatial information. This work introduces a novel multi-confocal FCS (mFCS) technique that allows simultaneous FCS measurements in different locations within a cell. It is based on the use of a spatial light modulator (SLM) to create several distinct observation volumes at a time and an electron-multiplying charge coupled device (EMCCD) camera to perform parallel detection. The spatial resolution as well as the sensibility of the mFCS system are close to that of a classical FCS setup and using a special readout mode, a temporal resolution of $14mu s$ is reached. The mFCS technique is applied to study the cellular response to thermal stress by monitoring the transcription factor heat shock factor 1 (HSF1), which is a key regulator of the heat shock response. mFCS experiments in living cells reveal changes in the dynamics of HSF1 upon heat shock. These changes concern the affinity as well as the spatial homogeneity of its interactions with DNA. Additionally, the performance of a CMOS-SPAD camera, consisting of an array of single photon avalanche diodes, is evaluated and the device is tested as an alternative detector for mFCS in living cells.

Les capteurs CMOS sont de plus en plus présents dans les produits de grande consommation comme les téléphones portables. Les images issues de ces capteurs nécessitent divers traitements numériques avant d'être affichées. Aujourd'hui, seuls des composants dédiés sont utilisés pour maintenir un niveau de consom- mation électrique faible. Toutefois leur flexibilité est fortement limitée et elle ne permet pas l'intégration de nouveaux algorithmes de traitement d'image. Ce manuscrit présente la conception et la validation de l'archi- tecture de calcul eISP entièrement programmable et originale capable de supporter la vidéo HD 1080p qui sera intégrée dans les futures générations de téléphones portables.

The BRIght Target Explorer (BRITE) constellation is revolutionary in the sense that the same scientific objectives can be achieved smaller (cm3 versus m3 ) and lighter (< 10kg versus 1, 000kg). It is a space astronomy mission, observing the variations in the apparent brightness of stars. The work presented herein focuses on the assembly, integration and test of the instrument used on-board six nanosatellites that form the constellation. The instrument is composed of an optical telescope equipped with a Charge Coupled Device (CCD) imager and a dedicated computer. This thesis provides a particular in-depth look into the inner workings of CCD. Methods used to characterize the instrument CCD in terms of its bias level stability, gain factor determination, saturation, dark current and readout noise level evaluation are provided. These methodologies are not limited to CCDs and they provide the basis for anyone who wishes to characterize any type of imager for scientic applications.