This thesis examines representations of the French educational system in Senegal as presented in L'aventure ambiguë and L'appel des arènes. Each unfolding respectively within a colonial and postcolonial Senegalese context, the novels problematize the French school system by incorporating representations of its failures. As this thesis will argue, analyzing each author's educational discourse will unmask a Senegalese perspective on a French institution, showcase various ways that Senegalese students internalized their educational experience and provide representations of the ways in which French education could be, and was, utilized by its pupils. Using two African novels in French to interpret historical experience will facilitate understanding of the French educational system from a Senegalese perspective. The first chapters create a foundation for analysis: Chapter two explains French goals and objectives when implementing a formal educational system in West Africa, while chapter three explores the form and function of the African novel in order to present it as a useful historical tool. Having defined the African novel in French as a viable means to interpret historical experience, chapter four focuses analysis on revealing how a system that was meant to procure French dominance, was ultimately transformed into a tool for Senegalese advantage. / Master of Arts

Salmonellosis, a significant foodborne illness in humans, is caused by Salmonella, with poultry and poultry products acting as significant reservoirs and sources of human infection. Salmonella enterica subspecies enterica serotype Reading has recently emerged as a notable foodborne pathogen responsible for extensive multistate human outbreaks in North America. This study focused on evaluating the capacity of the emerged serotype to colonize broiler breeder reproductive tissues and potentially contaminate eggs, indicating the potential for vertical transmission. For this investigation, two Salmonella Reading strains were utilized, one associated with outbreaks and another non-outbreak strain. Both strains were initially modified with bioluminescent marker genes to facilitate tracking post-experimental infection in broiler breeders. The results indicated that both strains could colonize the reproductive tract of infected hens and be transmitted vertically through the eggs. This finding enhances our understanding of the colonization and vertical transmission capabilities of this serotype in broiler breeders.

Streptococcus pneumoniae (pneumococcus), a gram-positive bacterium, is commensal to the human nasopharynx. It is also a common cause of respiratory tract infections and multiple invasive diseases worldwide. Pneumococci attach to the nasopharynx, lung, and vascular endothelial cells, which contributes to colonization as well as to the development of pneumonia, bacteremia, and meningitis. Choline binding proteins (CBPs) are a unique set of cell wall proteins conserved within pneumococci. CBPs bind noncovalently to the phosphocholine of the cell wall through choline binding domains. The choline binding domains of the CBPs are highly conserved; however, they are diverse in their affinities and functions due to differences in their functional domains. Several of the CBPs are predicted to play a role in adherence and colonization, though direct evidence of binding to epithelial receptors is lacking. This project focuses on the ability to express and purify some of the lesser characterized CBPs which are predicted to serve as adhesins and to identify their cognate ligand proteins on host cells with the hope of identifying novel bacterial-host interactions that contribute to colonization.

Esta tese examina a experiência de colonização da colônia Dona Francisca, um dos maiores e mais importantes núcleos de colonização alemã do Brasil no século XIX. Fundada em 1851, na região nordeste de Santa Catarina, a colônia Dona Francisca, hoje município de Joinville, foi concebida e organizada pela Sociedade Colonizadora de 1849 em Hamburgo, empresa alemã especificamente constituída para colonizar parte das terras pertencentes ao patrimônio dotal da Princesa Dona Francisca em Santa Catarina. As principais fontes de arquivo utilizadas neste estudo compreendem um conjunto variado de documentos: títulos e listas de proprietários de terras, contratos de concessão, compra e venda de terrenos, correspondências de imigrantes, livros-caixa e documentos da direção da colônia, crônicas e jornais de época e relatórios da direção da Sociedade Colonizadora de 1849 em Hamburgo. As evidências apresentadas nesta tese questionam e complementam os estudos da história de Joinville e da formação econômica de Santa Catarina. Ao mesmo tempo, amplia o escopo de análise da história econômica do Brasil ao oferecer dados quantitativos, estatísticas e indicadores que permitem avaliar o grau de concentração da posse da terra e o desenvolvimento econômico inicial de Joinville, uma economia organizada a partir do trabalho livre do colono europeu e da pequena produção mercantil voltada aos abastecimentos interno e externo. / This thesis has examined the experience of colonization of the Dona Francisca colony, one of the largest and most important centers of German colonization in Brazil in the nineteenth century. Established in 1851 in the northeast region of Santa Catarina (Brazil), the Dona Francisca colony, current Joinville city, was conceived and organized by the \"Colonization Society of 1849 in Hamburg\", a German company specifically constituted in order to colonize part of the lands, which belonged to the Princess Dona Francisca\'s nuptial patrimony, in Santa Catarina. The main archive sources used in this research have included a varied set of documents. Among them are titles and lists of landowners, contracts of concession, buying and selling of lands, immigrants\' correspondence, and book of accounts and of documents of the colony direction, memorial and journal from that time beyond reports from Colonization Society of 1849, in Hamburg. The presented evidences in this thesis have questioned and complemented the studies on History of the Joinville city as well as the economic formation of Santa Catarina. Simultaneously, it has expanded the scope of Brazilian economic history analysis by offering quantitative data, statistics, and indicators that allow evaluating the concentration of land property and initial economic development of Joinville city, of which economy was organized from the free labor of the European colonists and small mercantile production that supplied both internal and external markets.

[Truncated abstract] Spontaneous plant growth and soil development occur at disturbed sites with their extent and nature being variously affected by soil fertility status, local climate and topographic conditions. Soil-plant interactions can be diverse and site-specific within a disturbed landscape. The main purpose of the present study is to evaluate soil characteristics and landscape indices in relation to natural plant growth and soil development under different conditions and for diverse materials. A comprehensive study has been carried out to evaluate spontaneous soil development and plant colonization on various regolith materials at a railway cutting near Jarrahdale bauxite mine and on various substrates comprising waste rock, weathered regolith and replaced topsoil at Scotia (Norseman, Western Australia) and Kelian (East Kalimantan, Indonesia). At Jarrahdale soil development has occurred slowly over 36 years in relation to morphological changes in surface horizons. Soils at several locations exhibit substantial changes in color, texture and structure. The slow soil development is primarily due to low biomass and litter contributions (˜1 Mg/ha) from colonizing plants (e.g. Dryandra sessilis, Eucalyptus marginata and low shrubs) on the cutting shelf and slow litter decomposition. Nutrient accumulation is up to 5 kg N/ha, and 0.5 kg/ha for P and K. Surface soil samples from Jarrahdale are generally acidic (pH < 5.1) and contain low concentrations of total soil carbon (20 g/kg) and nutrients of total nitrogen (0.73 g/kg), bicarbonate-extractable phosphorus (bic-P) (< 2 mg/kg), bic-K (37 mg/kg) and total exchangeable bases (<1.1 cmol/kg, with 24 % base saturation). Soil properties at the Scotia waste dump are mainly associated with alkaline (mean pH = 9) and saline conditions (EC1:5 = 1.01 dS/m). Exchangeable base values are high with average concentrations of exchangeable Ca of 18 cmol/kg and exchangeable Mg of 6 cmol/kg, thus these elements are not a limiting factor for plant nutrition. Patchy plant growth on the waste dump is mostly related to differences in water availability in the arid region and to salinity such that halophytes (saltbushes Maireana and Atriplex) colonize many parts of the waste dump together with some Melaleuca and Eucalyptus species

Thesis (MA)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: This study focuses on the nascent colonial tourist sector of the Belgian Congo from 1945 until independence in 1960. Empire in Africa was the last remaining vestige of might for the depleted European imperial powers following the Second World War. That might, however, was largely illusory, especially for Belgium, which had been both defeated and occupied by Germany. Post-war Belgium placed much value on its colonial role in the Belgian Congo, promoting and marketing its imperial mission to domestic and international audiences alike. Such efforts allowed Belgium to justify a system that was under fire from the new superpowers of the United States of America (USA) and the Soviet Union. This thesis makes the case that the Belgian authorities recognised the opportunity to harness the ‘new’ economic activity of tourism to help deliver pro-colonial propaganda, particularly to the USA which had a growing affluent class and where successive administrations were keen to encourage overseas travel. In building a tourism sector post the Second World War, efforts in diversifying the economy were secondary to the objective of using the marketing of tourism to actively position and promote Belgium’s long-term involvement in the Congo.

Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Subject of study: Specialist in laboratory methods Student: Bc. Miroslava Hošková Supervisor: RNDr. Klára Konečná, Ph.D. Title: Microbiological diagnostics of causative agents of infectious diseases associated with central venous catheter insertion Background: The purpose of the thesis is to provide a comprehensive insight into the issue of microbiological diagnosis of agents that cause central venous catheter related infectious processes. The primary objective of the thesis is to evaluate the frequency of occurrence of individual microbiological agents and draw a comparison between published reports and empirical findings. The secondary objective of the thesis is to consider the extent of central venous catheter microbial colonization, i.e., in how many cases of positive culture cultivation, their contamination or significant colonization have been determined, and eventually whether system catheter infection has been proved in correlation with the results of blood culture. Methods: One hundred and seventy-one central venous catheters from different inpatient departments were delivered to the microbiological section of hospital Nemocnice Boskovice s. r. o. for culture examination have been...

[ES] Las bacterias resistentes a múltiples antibióticos, como el Enterococo resistente a vancomicina (ERV), son un problema creciente en los pacientes hospitalizados, por lo que se necesita estrategias alternativas para combatir estos patógenos. Las infecciones causadas por ERV suelen comenzar con la colonización del tracto intestinal, un paso crucial que se afectado por la presencia de la microbiota. Sin embargo, los antibióticos alteran la microbiota y esto promueve la colonización de ERV. Una vez que el patógeno ha colonizado el intestino, alcanza niveles muy altos pudiendo diseminar a otros órganos y pacientes. A pesar de su importancia, se sabe muy poco sobre los genes que codifica para colonizar el intestino y sobre el mecanismo por el cual la microbiota suprime su colonización intestinal, siendo los dos objetivos principales. En primer lugar hemos utilizado una metodología previamente descrita (Zhang et al., 2017, BMC Genomics), basada en la generación de una librería de mutantes por transposición junto a secuenciación masiva, con el fin de identificar los genes codificados por ERV necesarios para la colonización del intestino en ratones. Además, hemos realizado análisis metatranscriptómicos para identificar aquellos genes más expresados. El análisis ha identificado genes cuya interrupción reduce significativamente la colonización intestinal en el intestino grueso. Los genes que más afectaron a la colonización codifican proteínas relacionadas con la absorción o el transporte de diversos nutrientes como los carbohidratos (subunidad EIIAB del transportador PTS de manosa, el regulador transcripcional de la familia LacI, ácido N-acetilmurámico 6-fosfato eterasa) o iones (proteína transportadora dependiente de ATP (ABC) y proteínas del grupo [Fe-S]). El papel de estos genes en la colonización se ha confirmado mediante experimentos de mutagénesis directa y de competición con la cepa salvaje. Además, estos genes afectan a la colonización intestinal con diferentes antibióticos (clindamicina y vancomicina). Para identificar el mecanismo molecular por el cual cada gen afecta a la colonización, hemos realizado experimentos in vitro y ex vivo además del análisis transcriptómico. Los experimentos in vitro confirman que las proteínas del grupo [Fe-S] están involucradas en el transporte iones de hierro, principalmente Fe3+. Por otra parte, los genes de la subunidad EIIAB del transportador de manosa y del ácido N-acetilmurámico 6-fosfato eterasa son necesarios para la utilización de la manosa y el ácido N-acetilmurámico, respectivamente, azúcares que suelen estar presentes en el intestino. También confirmamos que el regulador transcripcional de la familia LacI es un represor que afecta a proteínas transportadoras ABC, probablemente implicadas en la absorción de carbohidratos. Además, algunos de estos genes están codificados principalmente por cepas clínicas de E. faecium y en menor medida por cepas comensales. En segundo lugar, estudiamos los mecanismos de protección de un consorcio de cinco bacterias comensales, que anteriormente se había demostrado que disminuían la colonización intestinal por ERV en ratones. Mediante transcriptómica, metabolómica y los ensayos in vivo observamos que el consorcio bacteriano inhibe el crecimiento de ERV mediante la reducción de nutrientes, concretamente fructosa. Por último, el análisis ARN-Seq in vivo de cada aislado en combinación con los ensayos ex vivo e in vivo demostraron que una sola bacteria (Olsenella sp.) proporciona protección. En conjunto, los resultados obtenidos han identificado la función de genes específicos requeridos por ERV para colonizar el intestino. Además, hemos identificado un mecanismo mediante el cual la microbiota confiere protección. Estos resultados podrían conducir a nuevos enfoques terapéuticos para prevenir las infecciones causadas por este patógeno multiresistente a los antibióticos. / [CA] Els bacteris resistents a múltiples antibiòtics, com el Enterococo resistent a vancomicina (ERV), són un problema creixent en els pacients hospitalitzats, que són resistents a la majoria d'antibiòtics disponibles per la qual cosa es necessita estratègies alternatives per a combatre aquests patògens. Les infeccions causades per ERV solen començar amb la colonització del tracte intestinal, un pas crucial que es veu afectat per la presència de la microbiota. No obstant això, els antibiòtics alteren la microbiota i això promou la colonització de ERV. Una vegada que el patogen ha colonitzat l'intestí, aconsegueix nivells molt alts podent disseminar a altres òrgans i pacients. Malgrat la seua importància, se sap molt poc sobre els gens que codifica ERV per a colonitzar l'intestí i sobre el mecanisme pel qual la microbiota suprimeix la seua colonització intestinal. En primer lloc hem utilitzat una metodologia prèviament descrita (Zhang et al., 2017, BMC Genomics), basada en la generació d'una llibreria de mutants per transposició junt amb seqüenciació massiva, amb la finalitat d'identificar els gens codificats per ERV necessaris per a la colonització de l'intestí en ratolins. A més a més, hem realitzat anàlisi metatranscriptòmics per a identificar aquells gens més expressats. L'anàlisi ha identificat gens quina interrupció redueix significativament la colonització intestinal en l'intestí gros. Els gens que més van afectar la colonització codifiquen proteïnes relacionades amb l'absorció o el transport de diversos nutrients com els carbohidrats (subunitat EIIAB del transportador PTS de manosa, el regulador transcripcional de la família LacI, àcid N-acetilmuràmic 6-fosfat eterasa) o ions (proteïna transportadora dependent d'ATP (ABC) i proteïnes del grup [Fe-S]). El paper d'aquests gens en la colonització s'ha confirmat mitjançant experiments de mutagènesis directa i de competició amb el cep salvatge. A més, aquests gens afecten la colonització intestinal amb diferents antibiòtics (clindamicina i vancomicina). Per a identificar el mecanisme molecular pel qual cada gen afecta a la colonització, hem realitzat experiments in vitro i ex viu a més de l'anàlisi transcriptòmic. Els experiments in vitro confirmen que les proteïnes del grup [Fe-S] estan involucrades en el transport d'ions de ferro, principalment Fe3+. D'altra banda, els gens de la subunitat EIIAB del transportador PTS de manosa i de l'àcid N-acetilmuràmic 6-fosfat eterasa són necessaris per a la utilització de la manosa i l'àcid N-acetilmuràmic, respectivament, sucres que solen estar presents en l'intestí. També confirmem que el regulador transcripcional de la família LacI és un repressor que afecta proteïnes transportadores ABC, probablement implicades en l'absorció de carbohidrats. A més a més, alguns d'aquests gens estan codificats principalment per ceps clínics de E. faecium i en menor mesura per ceps comensals. En segon lloc, estudiem els mecanismes de protecció d'un consorci de cinc bacteris comensals, que adès s'havia demostrat que disminuïen la colonització intestinal per ERV en ratolins. Amb l'ús de transcriptòmica, metabolòmica i els assajos in vivo observem que el consorci bacterià inhibeix el creixement de ERV mitjançant la reducció de nutrients, concretament fructosa. Finalment, l'anàlisi ARN-Seq in vivo de cada aïllat en combinació amb els assajos ex viu i in vivo van demostrar que un sol bacteri (Olsenella sp.) proporciona protecció. En conjunt, els resultats obtinguts han identificat la funció de gens específics requerits per ERV per a colonitzar l'intestí. A més, hem identificat un mecanisme mitjançant el qual la microbiota confereix protecció. Aquests resultats podrien conduir a nous enfocaments terapèutics per a previndre les infeccions causades per aquest patogen multiresistent als antibiòtics. / [EN] Multidrug-resistant bacteria, such as vancomycin-resistant-Enterococcus (VRE), are an increasing problem in hospitalized patients. Some VRE strains can be resistant to most available antibiotics, thus, alternative strategies to antibiotics are urgently needed to combat these challenging pathogens. Infections caused by VRE frequently start by colonization of the intestinal tract, a crucial step that is impaired by the presence of the intestinal microbiota. Administration of antibiotics disrupts the microbiota, which promotes VRE intestinal colonization. Once VRE has colonized the gut, it reaches very high levels, which promotes its dissemination to other organs and its transfer to other patients. Despite the relevance of VRE gut colonization, very little is known about the genes encoded by this pathogen to colonize the gut and about the mechanisms by which the microbiota suppresses VRE gut colonization. In this thesis, we have utilized a previously described methodology (Zhang et al., 2017, BMC Genomics), based on the generation of a transposon mutant library coupled with high-throughput sequencing, in order to identify VRE encoded genes required for colonization of the mouse intestinal tract. In addition, we have performed metatranscriptomic analysis in mice to identify VRE genes specifically expressed in the gut. Our analysis has identified genes whose disruption significantly reduces VRE gut colonization in the large intestine. The genes that most affected VRE gut colonization encoded for proteins related to the uptake or transport of diverse nutrients such as carbohydrates (PTS mannose transporter subunit EIIAB, LacI family DNA-binding transcriptional regulator, N-acetylmuramic acid 6-phosphate etherase) or ions (phosphate ABC transporter ATP-binding protein and proteins from [Fe-S] cluster). The role of these genes in gut colonization has been confirmed through targeted mutagenesis and competition experiments against a wild type strain. Moreover, these genes affect gut colonization under different antibiotic treatments (clindamycin and vancomycin). To elucidate the mechanism by which each gene influences gut colonization, we have performed in vitro and ex vivo experiments besides transcriptomic analysis. In vitro experiments confirm that proteins from [Fe-S] cluster are involved in the transport of different forms of iron ions, mostly Fe3+. On the other hand, the PTS mannose transporter subunit EIIAB and N-acetylmuramic acid 6-phosphate etherase genes are required for the utilization of mannose and N-acetyl-muramic acid, respectively, sugars that are usually present in the intestinal environment. We have also confirmed that LacI family DNA-binding transcriptional regulator is a repressor that affects the expression of genes encoding for an ABC transporter probably involved in the uptake of carbohydrates. Furthermore, we have confirmed that some of these genes are encoded mainly by E. faecium clinical strains but not or to a lower extent by commensal strains. Secondly, we studied the mechanisms of protection of a consortium of five commensals bacteria, previously shown to restrict VRE gut colonization in mice. Functional transcriptomics in combination with targeted metabolomics and in vivo assays performed in this thesis indicated that the bacterial consortium inhibits VRE growth through nutrient depletion, specifically by reducing the levels of fructose. Finally, in vivo RNA-Seq analysis of each bacterial isolate of the consortium in combination with ex vivo and in vivo assays demonstrated that a single bacterium (Olsenella sp.) could recapitulate the protective effect. Altogether, the results obtained have identified the function of specific genes required by VRE to colonize the gut. In addition, we have identified a specific mechanism by which the microbiota confers protection against VRE colonization. These results could lead to novel therapeutic approaches to prevent infections caused by this pathogen. / Flor Duro, A. (2021). Characterization of Genes and Functions Required by Multidrug-resistant Enterococci to Colonize the Intestine [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/166494

In this thesis I consider the interface between copyright law and cultural practice. I argue that the protection of folklore through copyright obfuscates the status of folklore as a generative resource for derivative works in favour of its status as a carrier of national identity, over which states can exercise property rights. Specifically, I analyse the significance of folklore within the playwriting culture of Ghana and discuss how, within this specific context, the introduction of the 2005 Copyright Act (which requires nationals to seek permission and pay a fee to use folklore), rather than incentivising artists to create derivative works from folklore, significantly disrupts the ability of playwrights to continue to create work that reflects the codified theatrical practice established in Ghana post independence. As such, the Ghana Copyright Act, 2005 threatens to jeopardise the fundamental balance in copyright between protection and access, and so the purpose of copyright as a mechanism for incentivising artists. Through exploring the development of the relationship between folklore and copyright and how protection for folklore interacts at the international, continental and sub-regional levels, this thesis examines both the potential impact of the copyright law in Ghana and the efficacy of protecting folklore through a copyright paradigm at all.

When President Sebastián Piñera entered office in 2010 the Mapuche indigenous people were receiving two contrasting messages from the Chilean State. On the one hand, the government ratified ILO Convention 169, pledging to protect the indigenous right to prior consultation in programs that affect their communities. On the other hand, the government was involved in the oppression of Mapuche communities in the region of the Araucanía through militarisation and the application of the Anti-Terrorist Law to punish radical Mapuche activists that protest corporate encroachment on their land. While Piñera had the opportunity to legitimize the Mapuche demand for self-determination by implementing ILO Convention 169 according to international standards and putting an end to the “Mapuche Conflict,” instead the situation of the Mapuche political movement worsened under his leadership. The Piñera administration used indigenous rights policy and a discourse of terrorism as a strategy to delegitimize the Mapuche demand for self-determination in order to protect corporate profitability, a key factor in the Chilean neoliberal economic project.