Die Anwendung von Biokohlen in der Landwirtschaft gewinnt durch die positiven Aspekte der Kohlenstoffsequestrierung, Bodenverbesserung und eines erhöhten Pflanzenwachstums in den letzten Jahren an Bedeutung. Deshalb geht die vorliegende Arbeit den Fragen nach, welche Wirkungen unterschiedliche Biokohlen in Kombination mit oder ohne Gärrest und / oder Stickstoffdünger auf die Bodenchemie, Bodenbiologie und Wachstum, Entwicklung, Ertrag, Ertragsstruktur, Nährstoffe sowie Qualität von Nutzpflanzen haben. Außerdem wurden die Effekte unterschiedlicher Biokohlen auf die Wurzelmorphologie von Sommerweizen quantifiziert. Eine weitere Frage war, inwiefern Biokohlen Stickstoffquellen sorbieren. Es wurden ein Feldversuch und mehrere Gefäßversuche durchgeführt, um die Änderungen der Bodenchemie, den Einfluss auf die Bodenbiologie am Beispiel von Collembolen und die Pflanzenparameter zu bestimmen. Für die Wurzeluntersuchungen wurden Rhizoboxversuche durchgeführt und zur Quantifizierung der Stickstoffsorption ein in vitro Versuch angelegt. Die Ergebnisse der Feld- und Gefäßuntersuchungen zeigten, dass die Biokohlen die Bodeneigenschaften positiv beeinflussten. Die Biokohlen nahmen keinen konsistenten Einfluss auf die Erträge von Kulturpflanzen. Die Nährstoffgehalte der Kulturpflanzen konnten zum Teil positiv beeinflusst werden. Die Collembolenabundanzen zeigten sowohl in der Feldstudie als auch im Gefäßversuch keine signifikanten Unterschiede zwischen den getesteten Biokohlen. Hohe Mengen der fermentierten HTC-Biokohle führten zu negativen Wirkungen auf die Collembolenabundanz im Gefäßversuch. Die Rhizoboxversuche zeigten einen positiven Einfluss der Pyro-Biokohle auf die oberirdische und unterirdische Trockenmasse sowie die Wurzelmorphologie von Sommerweizen. Unterschiedliche Stickstoffquellen wurden von der HTC-Biokohle stärker sorbiert als von der Pyro-Biokohle. Generell lässt sich aus den differenzierten Wirkungen der Biokohlen weitere Forschung mit dem Fokus Boden ableiten. / Over the past few years the use of biochars in agriculture has gained more importance due to positive effects on carbon sequestration, soil improvement and increased plant growth. Therefore, the present work pursues the question which effects different biochars have in combination with or without digestate and / or nitrogen fertilizer on soil chemistry, soil biology and growth, development, yield, yield components, nutrients and quality of crops. In addition, the effects of different biochars on root morphology of spring wheat were quantified. Further to elucidate potential mechanisms underlying biochar effects on crops it was studied how biochars sorb various nitrogen sources. A field test and several pot experiments were conducted to determine the changes in soil chemistry, the impact on soil-dwelling Collembola and plant parameters. For the root studies rhizobox experiments were performed. To quantify the nitrogen sorption of biochars an in vitro experiment was conducted. The results of the field test and pot experiments showed that biochars positively affected soil chemical characteristics. However, yields of crops were not consistently affected by biochars. The nutrient content of crops were positively influenced to some extent. The abundance of Collembola was not significantly influenced by the biochars tested in the field study and in the pot experiment. High amounts of fermented HTC-biochar had negative effects on the abundance of Collembola in the pot experiment. Rhizobox experiments showed a positive influence of Pyro-biochar on the aboveground dry matter, belowground dry matter and root morphology (e.g. root length) of spring wheat. Amount and reversibility of nitrogen sorption was dependent on the type of biochar with HTC-biochar sorbing more nitrogen than Pyro- biochar. Generally it can be concluded that because of the different effects of biochars further research with a focus on soil is needed.

Thesis (M.Sc.)--University of Hong Kong, 1998. / Includes bibliographical references (leaf 61-62) Also available in print.

Made available in DSpace on 2014-06-11T19:26:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-04-12Bitstream added on 2014-06-13T18:29:56Z : No. of bitstreams: 1 ranzani_c_me_bauru.pdf: 2094729 bytes, checksum: c210f6a979b7defe9f252348fef04a1c (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Para que as organizações sejam competitivas e aumentem as chances de sobrevivência no mercado competitivo, é necessária a gestão do processo produtivo cada vez mais eficiente. Nesta gestão são utilizados sistemas focados em qualidade, meio ambiente, segurança e saúde no trabalho, entre outros. A integração desses sistemas otimizam recursos, processos e melhoram a imagem da organização, influenciando, assim, a lucratividade. Esta dissertação tem como objetivo formular diretrizes para o planejamento e implementação de sistema de gestão integrada em empresas da construção civil. Para a elaboração deste trabalho, foi adotada a pesquisa qualitativa, exploratória, bibliográfica com a realização de dois estudos de caso sobre os elementos do sistema de gestão integrada de duas construtoras certificadas em ISO 9001, ISO 4001 e OHSAS 18001 fazendo com que o pesquisador, se ambiente ao universo de estudo. Um referencial teórico foi apresentado sobre as características do processo produtivo, sistema de gestão da qualidade, meio ambiente, saúde e segurança no trabalho e integração desses. Conclui-se que, o objetivo traçado, foi devidamente alcançado, pois foram apresentados os elementos do sistema de gestão integrada nas duas construtoras, sendo identificadas as boas práticas e pode-se propor, diretrizes para o planejamento e implamentação do sistema de gestão integrada em empresas da construção civil / To ensure that organizations are competitive and increase the changes of survival in competitive market, it is necessary to management of the productive process ever more efficiently. This management systems are used raised in quality, environment, health and safety at work, among others. The integration of these systems optimize resources, process, and improve the image of the organization, influencing, thus, the profitability. This dissertation has like objetctive, formulate guidelines for the planning and implementation of integrated management system in civil construction companies. For this work was adopted the qualitative research, exploratory, bibliographic with the achievement of two case studies about the elements of the integrated management system of two construction companies certifieds in ISO 9001, ISO 14001, and OHSAS 18001 making the researcher, environment the universe of study. A theoretical reference was presented about the characteristics of the production process, a system of quality management, environment, health and safety at work and integration of these. It concludes that, the outlined objective was duly achieved, because the elements of integrated management system were submitted in the two construction companies, being identified the good practices and could propose, guidelines for the planning and implementation of integrated management system in civil construction companies

Jordens klimat varierar normalt över tid, men aldrig tidigare har klimatförändringarna varit så snabba och påtagliga. Stigande temperaturer, förhöjda havsnivåer och hotade ekosystem är till stor del en följd av människans miljöpåverkan som genom sitt utnyttjande av resurser och utsläpp bidrar till den utökade växthuseffekten. Alla berörs och samtliga måste ta sitt ansvar för att förhindra att detta fortskrider. Genom att förstå miljökonsekvenserna av sitt agerande kan bättre förutsättningar skapas för kommande generationer. Studier visar att byggbranschen står för nästan en tredjedel av de totala utsläppen av växthusgaser, men att det fortfarande finns kunskapsluckor kring var miljöpåverkan är som störst. Genom klimatavtal, branschöverskridande forum och verktyg i form av livscykelanalyser och miljövarudeklarationer kan kunskapen lyftas. Ett flertal lättillgängliga verktyg med generiska data har utvecklats för livscykelanalyser av byggnader, men för anläggningssektorn finns ännu inte helt anpassade verktyg. I nuläget väljs grundläggningsmetod mestadels utifrån den ekonomiska aspekten, vilket bidrar till att miljöaspekten inte får önskvärt utrymme. Denna studie är en fallstudie som söker svar på vilken grundläggningsmetod som lämpar sig bäst ur ett hållbart och ekonomiskt perspektiv i en specifik jordprofil av lera som är vanligt förekommande kring Uppsala. För att lyfta kunskapen kring miljöpåverkan, jämfördes fyra vanliga grundläggnings-metoder: inblandningspelare med Multicem och lättfyllnadsmetoderna; lättklinker, skumglas och cellplast. Utifrån sättningsberäkningar i jordprofilen kunde dimensioneringar utföras av materialåtgång för respektive metod. Dimensioneringen användes sedan för att utföra kostnadsuppskattningar och beräkningar av klimatpåverkan, i form av koldioxidekvivalenter, under produktframställning och transporter till den specifika platsen. Utöver detta undersöktes bedömningen av det kemiska innehållet genom två miljödatabaser, BASTA och SundaHus. Resultatet visade att skumglas har den lägsta totala klimatpåverkan, där ungefär hälften av koldioxid¬utsläppen kommer från produktionen och resterande del från transporter. Det tyder på att det geografiska läget för grundläggningen spelar en väsentlig roll för resultatet av klimatpåverkan. Skumglas erhåller bästa betyg i BASTA och SundaHus men har den högsta totalkostnaden av de jämförda alternativen i studien. Inblandningspelare med Multicem som bindemedel har den lägsta totalkostnaden och den näst lägsta klimatpåverkan. Genom intervjustudien konstaterades att val av grundläggningsmetod främst avgörs av kostnaden vilket innebär att Multicem förmodligen skulle väljas i första hand. Utifrån kemiskt innehåll är bedömningen däremot ofullständig, eftersom Multicem ännu inte finns deklarerad i SundaHus. Sammanfattningsvis beror valet av grundläggningsmetod på ett flertal faktorer där företag måste värdera vilken faktor som har högst prioritet. / The climate normally varies over time but never before has the climate change been so rapid and tangible. Rising temperatures, elevated sea levels and endangered ecosystems are consequences of human impact on the environment. The use of resources and emissions contributes to the increased impact on the greenhouse effect. Everyone is affected by the climate change and must take his or her responsibility to create better conditions for future generations by understanding the environmental consequences of his or her actions. Studies reveal that the construction industry accounts for almost one third of the total greenhouse gas emissions but there are still gaps in knowledge about the environ¬mental impact. The knowledge can increase through agreements on climate actions, industry-wide forums and tools such as life-cycle assessments and environmental product declarations. Several available tools with generic data have been developed for life-cycle assessments of buildings, but for the infrastructure sector the tools are still not fully developed. Today, the foundation method is mostly chosen based on the economical aspect, and the environmental aspect is not given priority. The aim of this case study is to investigate which foundation method is best suited from an environ-mentally sustainable and economical perspective in a specific soil profile of clay which is common around Uppsala. To raise awareness of environmental impact, four common foundation methods were compared: deep soil mixing with Multicem and light filling methods; expanded clay lightweight aggregate, foam glass and expanded polystyrene foam. Based on settlement calculations in the soil profile, dimensions could be made for consumption of material for each method. The consumption of material was used to make estimations of costs and calculations of climate effect, in terms of carbon dioxide equivalents, during production of the material and transport to the specific location. In addition, the assessment of the chemical content was examined through two environmental databases, BASTA and SundaHus. The result revealed that foam glass has the lowest total climate effect, where about half of the carbon dioxide emissions come from production and the remainder from transport. This indicates that the geographical location of the foundation is important for the result of the climate impact. Foam glass receives the best ratings in BASTA and SundaHus but has the highest total cost of the compared alternatives in the study. Deep soil mixing with Multicem as binder has the lowest total cost and the second lowest climate impact. Through the interview study, it was found that the choice of foundation method is mainly determined by the cost, which means that Multicem would probably be prefered. Based on chemical content the assessment is incomplete since Multicem has not been declared in SundaHus. In summary, the choice of foundation method depends on a number of factors in which companies must evaluate which factor has the highest priority.

The contents of the Thesis feature a survey of contemporary international attitude to environmental development and its effect on the educational trends in the Czech republic. Also, the short-term eco-educational project intended for primary school children is included here in the Thesis. The aim of the project is to arouse environmental awareness of children and excite their interest in this matter, which is considered to be the first essential step towards the sustainable development.

Whole cells of Vibrio cholerare serotype Inaba and serotype Ogawa (strains I389 and O395) were injected into rabbits to obtain antiserum. The antiserums were used for immune reaction with antigenic components of 25 strains of V.cholerae isolated from five provinces of Vietnam and the two standard strains I389 and O395 by Western-blot technique. Analysis of immune hybrid results showed that there were 11 antigenic components with molecular weights approximately 79kDa, 62kDa, 52kDa, 45kDa, 42kDa, 38kDa, 35kDa, 31kDa, 26kDa, 23kDa and 20kDa. In which the antigens of 45kDa, 42kDa, 31kDa and 20kDa were similar to OmpT, OmpS, Omp-31kDa and TcpA that have been considered as vaccine-candidate antigens. Among 25 V.cholerae strains, there were 6 antigenic components in common including 79kDa, 62kDa, 45kDa, 35kDa, 31kDa and 20kDa. 23/25 strains contained 42kDa antigen; 5/25 strains contained 38kDa and 23kDa antigens; 11/25 had 26kDa antigen. In addition, 7/25 strains contained antigens identical to V.cholerae I389 serotype Inaba; 6/25 strains contained antigens of I389 and O395; 12/25 strains had changes of antigenic components. These changes were actually the lack of antigens, not appearing new antigens. These results are considered as basis for researches about immune response and prevention of cholera disease. / Toàn bộ tế bào của các chủng Vibrio cholerare typ huyết thanh Inaba và typ huyết thanh Ogawa (chủng I389 và O395) được sử dụng để gây miễn dịch trên thỏ để thu kháng huyết thanh. Các kháng huyết thanh được dùng để thực hiện phản ứng miễn dịch với các thành phần kháng nguyên của 25 chủng V.cholerae phân lập từ 5 tỉnh thành của Việt Nam và hai chủng chuẩn I389 và O395 bằng kỹ thuật Western-blot. Phân tích kết quả lai miễn dịch cho thấy, có tổng số 11 thành phần kháng nguyên có kích thước khoảng 79kDa, 62kDa, 52kDa, 45kDa, 42kDa, 38kDa, 35kDa, 31kDa, 26kDa, 23kDa và 20kDa. Các kháng nguyên này chủ yếu là các protein màng ngoài (Omp) và kháng nguyên lông (TcpA). Trong đó các kháng nguyên 45kDa, 42kDa, 31kDa và 20kDa trùng với các kháng nguyên OmpS, OmpT, Omp-31kDa và TcpA được xem là những kháng nguyên dự tuyển vacxin tả. Có 6 kháng nguyên chung giữa 25 chủng với kích thước 79kDa, 62kDa, 45kDa, 35kDa, 31kDa và 20kDa. 7/25 chủng có các kháng nguyên giống với kháng nguyên của chủng V. cholerae I389 typ huyết thanh Inaba; 6/25 chủng có các kháng nguyên giống với kháng nguyên của cả hai chủng V.cholerae I389 và O395; 12/25 chủng có sự biến đổi thành phần kháng nguyên. Tuy nhiên, sự biến đổi này thực chất là sự thiếu hụt chứ không phải là sự xuất hiện các thành phần kháng nguyên mới. Các kết quả nghiên cứu này có thể được xem là nền tảng ban đầu cho các nghiên cứu về miễn dịch và dự phòng bệnh tả.

published_or_final_version / Environmental Management / Master / Master of Science in Environmental Management

Das Pflanzenwachstum fördernde Bakterien (PGPB) kommen ubiquitär sowohl an der Wurzel als auch am Spross der Pflanzen vor und sie können über direkte oder indirekte Mechanismen einen bedeutenden Beitrag zur Stickstoffernährung der Pflanzen leisten. Die vorliegende Arbeit umfasst a) die Isolierung von PGPB, welche das Wachstum verschiedener Pflanzenarten fördern und durch Fusarien verursachte Pflanzenkrankheiten bekämpfen, b) die Analyse der Möglichkeiten Probleme der Pflanzenernährung durch den Einsatz von PGPB zu lösen, c) die Entwicklung neuer molekularbiologischer Methoden zur Messung der Diversität und Aktivität der PGPB. Im Rahmen dieser Arbeit wurden Methoden zur Beschreibung der Diversität von rhizosphären PGPB entwickelt und verbessert um Verbindungen zwischen applizierten PGPB und deren Aktivitäten zu prüfen. Die sensitive quantitative real-time-PCR Methode wurde zur Quantifizierung bzw. zum Nachweis der inokulierten PGPB und zum Nachweis des nitrogenase-reduktase-Gens (nifH), des Markergens für potentiell diazotrophe Bakterien. Bakterienartspezifische Primer wurden aus dem Sequenzvergleich der 16S-23S ISR ausgewählter Bakterienstämme selektiert und Protokolle zur Quantifizierung dieser Bakterienarten erarbeitet. Die nifH Gen Quantifizierung an Pflanzen eröffnet die Möglichkeit Schlüsselorganismen in der assoziativen biologischen Luftstickstoffbindung zu identifizieren und kurzfristige Reaktionen der Bakteriengesellschaften auf Umweltveränderungen und Regulationsmechanismen in situ zu analysieren. / Plant growth promoting bacteria (PGPB) are ubiquitous in both plant root and shoot, and are important contributors to the nitrogen-input of plants exerting their positive effects on plant growth directly or indirectly through different mechanisms. The present work focuses on a) the isolation of PGPB, which promotes the growth of different plant cultures and controls plant diseases caused by Fusarium species, b) the prospects of PGPB to solve plant nutritional problems, c) developing new molecular methods for the assessment of their diversity and activity. In the frame of this thesis, the methods for the description of the diversity of root colonizing PGPB have been developed and improved to provide links between introduced PGPB abundance and activities. The approach used was based on the sensitive real – time PCR detection/quantification of introduced PGBP and the nitrogenase reductase gene (nifH), which served as a marker gene for potential diazotrophs. The amplified 16S-23S ISR sequences of studied bacteria were subjected to strain – specific primer design and a highly specific bacteria quantification protocol were developed. The bacteria quantification protocol was based on real – time PCR using strain specific primers in order to evaluate the colonization ability of studied bacteria, which were inoculated to plant roots. The results presented in this thesis have shown that monitoring of nifH amount in plant root is a suitable and promising approach to link inoculated diazotrophic bacteria abundance and its potential activity. The study of nifH gene abundance in plant offers the opportunity to identify key players in asymbiotic nitrogen fixation, to study short-term community responses in changing environments, or to analyze the effect of regulation in situ.

Die Anwendbarkeit der Verdünnungsmethode mit Hilfe des Markers Deuteriumoxid (D2O) zur in vivo-Erfassung der sich ändernden Körperzusammensetzung wurde an 53 Ziegenlämmern der Rasse Bunte Deutsche Edelziege im Gewichtsabschnitt von 4 kg bis 20 kg LM überprüft. Bei einer Lebendmasse von 4, 8, 12, 16 bzw. 20 kg wurde den Tieren nach einer 13- bis 18stündigen Nüchterung D2O injiziert und nach weiteren vier bis fünf Stunden Blutproben gezogen. Bei jeweils einem Teil der Tiere erfolgte anschließend die Schlachtung und die chemische Analyse der Körper zur Bestimmung des Ganzkörperwassergehaltes, der Chymusmasse und der Leerkörperzusammensetzung. Die Messungen der D2O-Konzentrationen in den Blutproben und die chemische Analyse der Ganzkörper ergaben eine Überschätzung des Ganzkörperwassergehaltes durch die Verdünnungsmethode um im Mittel 3,99 %. Zwischen dem chemisch bestimmten und dem geschätzten Wert besteht eine enge lineare Beziehung. Die Schätzung des Inhaltes des Gastro-Intestinaltraktes (Chymus) dient zur Schätzung der Leerkörpermasse. Dessen Variabilität lässt sich im Wesentlichen durch die Lebendmasse erklären. Die chemische Analyse der Tierkörper zeigte, dass die Zusammensetzung des fettfreien Leerkörpers im hohen Maß von der Leerkörpermasse abhängt. Anhand von allometrischen Regressionsgleichungen wurden die Bestandteile der fettfreien Leerkörpermasse geschätzt und daraus die Zusammensetzung des fetthaltigen Leerkörpers ermittelt. Der Vergleich der mittels chemischer Analyse bestimmten bzw. der Verdünnungsmethode geschätzten Leerkörperzusammensetzung ergab eine hohe Übereinstimmung beim Wasser-, Rohprotein- und Rohaschegehalt. Der Rohfettgehalt wies dagegen größere Abweichungen auf. Als Ursache wurde die Streuung der geschätzten Chymusmasse bzw. Chymuswassermasse aufgedeckt. Das Fazit ist, dass sich die D2O-Verdünnungsmethode zur in vivo-Bestimmung der sich ändernden Körperzusammensetzung von Ziegenlämmern im Gewichtsabschnitt von 4 kg bis 20 kg LM eignet. / On young goats (breed Bunte Deutsche Edelziege) with a live weight range of 4 kg up to 20 kg the suitability of the dilution method using the marker deuterated water (D2O) was checked in order to measure the changes of body composition during growth on living animals (in vivo). At a live weight of 4, 8, 12, 16 and 20 kg the animals received an intrave¬nous dose of D2O after a fastening time of 13 to 18 hours. Blood samples were taken after a waiting period of 4 to 5 hours. In connection with the last blood sample in each period a part of the animals were slaughtered to determine the total body water content, the digesta mass and the empty body composition by chemical analysis. The determination of the marker concentration in the blood samples and the chemical analysis of the total bodies showed that the dilution method overestimated total body water content by 3,99 %. There is a close linear relationship between the estimated and the chemically determined content of total body water. The estimation of the digesta mass serves for the estimation of the empty body mass. Its variation mainly depends on the live weight. The chemical analysis of the animal bodies revealed that the composition of the fat-free empty body is mainly dependent on the empty body mass. Using allometric equations the components of the fat-free empty body mass were estimated and thus the composition of the empty body could be calculated. The comparison of the chemically determined components with the estimated components of the empty body showed a high agreement concerning the content of water, protein and ash. However estimation of the fat content is less accurate. The reason is the variation of the estimated digesta mass and the digesta water mass. It can be concluded that the dilution technique using deuterated water is a practicable method to determine in vivo the changing body composition of growing kids with a live weight range of 4 kg up to 20 kg.

Orientador: Otávio José de Oliveira / Banca: Márcio Minto Fabricio / Banca: Adilson Renofio / Resumo: Para que as organizações sejam competitivas e aumentem as chances de sobrevivência no mercado competitivo, é necessária a gestão do processo produtivo cada vez mais eficiente. Nesta gestão são utilizados sistemas focados em qualidade, meio ambiente, segurança e saúde no trabalho, entre outros. A integração desses sistemas otimizam recursos, processos e melhoram a imagem da organização, influenciando, assim, a lucratividade. Esta dissertação tem como objetivo formular diretrizes para o planejamento e implementação de sistema de gestão integrada em empresas da construção civil. Para a elaboração deste trabalho, foi adotada a pesquisa qualitativa, exploratória, bibliográfica com a realização de dois estudos de caso sobre os elementos do sistema de gestão integrada de duas construtoras certificadas em ISO 9001, ISO 4001 e OHSAS 18001 fazendo com que o pesquisador, se ambiente ao universo de estudo. Um referencial teórico foi apresentado sobre as características do processo produtivo, sistema de gestão da qualidade, meio ambiente, saúde e segurança no trabalho e integração desses. Conclui-se que, o objetivo traçado, foi devidamente alcançado, pois foram apresentados os elementos do sistema de gestão integrada nas duas construtoras, sendo identificadas as boas práticas e pode-se propor, diretrizes para o planejamento e implamentação do sistema de gestão integrada em empresas da construção civil / Abstract: To ensure that organizations are competitive and increase the changes of survival in competitive market, it is necessary to management of the productive process ever more efficiently. This management systems are used raised in quality, environment, health and safety at work, among others. The integration of these systems optimize resources, process, and improve the image of the organization, influencing, thus, the profitability. This dissertation has like objetctive, formulate guidelines for the planning and implementation of integrated management system in civil construction companies. For this work was adopted the qualitative research, exploratory, bibliographic with the achievement of two case studies about the elements of the integrated management system of two construction companies certifieds in ISO 9001, ISO 14001, and OHSAS 18001 making the researcher, environment the universe of study. A theoretical reference was presented about the characteristics of the production process, a system of quality management, environment, health and safety at work and integration of these. It concludes that, the outlined objective was duly achieved, because the elements of integrated management system were submitted in the two construction companies, being identified the good practices and could propose, guidelines for the planning and implementation of integrated management system in civil construction companies / Mestre