Aus der Einleitung: "Ein wachsender Bedarf an nachhaltig verfügbaren Rohstoffen und knapper werdende Rohölressourcen steigern die wirtschaftliche Bedeutung natürlicher Faserstoffe. Das eröffnet neue, über den traditionellen Einsatz von Papier hinausgehende Anwendungen für cellulosische Fasern, beispielsweise in maßgeschneiderten 3D-Verpackungslösungen, im Leichtbau oder in Konstruktionswerkstoffen. Bei der Erschließung neuer Anwendungsbereiche steht neben der zentralen Kostenfrage das Ziel im Vordergrund, die Bauteile technologisch effizient herzustellen. Dafür sollen weitestgehend etablierte Technologien für die Verarbeitung der eingesetzten Halbzeuge einsetzbar sein, z.T. sind aber auch neue Technologien erforderlich, wie z.B. integriertes Fügen und Umformen. Diese Entwicklungen sind nicht auf die oft als Vorreiter angesehene Automobilbranche beschränkt, sondern betreffen z.B. auch die Möbelbranche, die Baustoffindustrie und zunehmend auch die Papierwirtschaft. ..."

La plupart des capteurs inertiels MEMS commerciaux comportent une masse d’épreuve et des moyens de transductions issus d’une même couche de silicium. Il en découle des compromis forts, notamment pour la détection capacitive : une couche épaisse permet d’augmenter la masse et donc de réduire le bruit brownien; inversement, une couche fine permet de réduire la taille des entrefers entre les électrodes, d’obtenir une variation de capacité plus importante, et donc de réduire la contribution du bruit électronique. Plusieurs composants MEMS multicouches ont déjà été réalisés et rapportés dans la littérature, mais aucun n’a cherché à augmenter la densité capacitive tout en réduisant le bruit thermomécanique. Pourtant, la disparition du compromis lié au procédé monocouche permet d’atteindre les hautes performances nécessaires aux applications émergeantes, en conservant la surface d’un capteur grand public.Cette thèse présente des accéléromètres multicouches à détection dans le plan et hors plan. Le procédé de fabrication combine une couche épaisse, dédiée à la réalisation de grandes masses d’épreuve, et une couche fine, permettant d’obtenir de fortes densités capacitives. Ces deux avantages, combinés à une détection par variation de surface, permettent d’obtenir une résolution de l’ordre du µg/rtHz, une grande gamme dynamique, tout en conservant une taille réduite. Le dimensionnement des capteurs a cherché à maximiser la gamme dynamique et minimiser le bruit en partant d’une taille fixée. D’abord analytique, il a été validé par des simulations par éléments finis.Le procédé de fabrication VLSI a été appliqué à des plaques 200mm. Plusieurs points critiques ont été rencontrés, notamment la surgravure des fonds de tranchée (notching). Combinée à la disparité de vitesse de gravure, elle a entrainé la destruction de beaucoup de capteurs hors plan. Ce problème a été résolu en amincissant la couche épaisse, entrainant une légère perte de performances.Les capteurs ont été caractérisés sur plaque (capacité statique, fréquence de résonance), puis au niveau puce (sensibilité, niveau de bruit, gamme dynamique). Ces dernières mesures ont nécessité le développement d’une électronique dédiée, à partir de composants discrets.Les accéléromètres dans le plan présentent une capacité statique et une fréquence de résonance très proches de la théorie. Ils atteignent une résolution de 8µg/rtHz pour une gamme dynamique de l’ordre de 160g. Cette dynamique de 145dB est fournie par un composant de seulement 0.24mm² ; elle est 100 fois plus élevée que la dynamique d’un composant grand public de même taille. De plus, la bande passante est importante et le capteur est lu en boucle ouverte.Les accéléromètres hors-plan présentent une forte fréquence de résonance, au-delà de 8kHz. La masse sismique plus fine, combinée à des ressorts plus larges, explique ce décalage par rapport au dimensionnement initial. Malgré la réduction de sensibilité induite, les capteurs présentent une résolution de 50 à 80µg/rtHz. L’encombrement est faible (jusqu’à 0.22mm²) et la gamme dynamique a été évaluée à plus de 200g. Dans le futur, des corrections de design et des améliorations dans le procédé de fabrication permettront d’utiliser l’épaisseur initialement prévue, afin d’harmoniser les performances avec celles de l’accéléromètre dans le plan et d’obtenir un accéléromètre 3-axes hautes performances.Ce type de capteurs pourrait jouer un grand rôle dans les applications émergentes en fournissant une bonne stabilité, un faible bruit et une grande gamme dynamique, tout en conservant l’empreinte d’un capteur grand-public.Ce nouveau procédé de fabrication montre donc déjà un gros potentiel à travers les premiers composants réalisés, mais ouvre également de nouvelles possibilités en termes de design. Dans le futur, il pourrait servir de plateforme technologique pour les capteurs inertiels, notamment les gyromètres, mais aussi pour les actionneurs, comme les micro-miroirs. / In the vast majority of commercial MEMS inertial sensors, both seismic mass and sensing elements are patterned in the same silicon layer. This sets stringent design trade-offs, in particular for a capacitive sensor: a large silicon thickness increases seismic mass and decreases the Brownian noise floor. A low silicon thickness on the other hand, allows smaller gaps between electrodes, higher capacitance variation and lower electrical noise floor. For this reason, several examples of multi-layer MEMS devices were presented in the past. Yet, increasing capacitance density while reducing mechanical noise floor has not been achieved so far. Breaking the single-layer trade-off could enable new emerging applications that require high-performance sensors within a consumer size.In this work, multi-layer, in-plane and out-of-plane accelerometer are presented. Thanks to the multi-layer process the devices can feature a thick layer for large inertial mass, as well as a thin layer for high capacitive density. These aspects, together with surface-variation detection, allow to obtain µg/√Hz resolution and large full-scale while keeping compact size.The sensors are designed through analytical modeling and finite elements method simulations in order to reach the highest dynamic range with the lowest noise at given footprint.Few critical aspects were encountered during the fabrication of the sensors, especially for out-of-plane accelerometers. The notching of the thick-layer etching coupled to the strong lag effect caused most of the z-axis sensors to fail. This forced a reduction of the process thickness and relative loss of performance for this type of sensors.The characterization of the sensors is performed both at wafer-level (static capacitance, resonance frequency) and at die level (scale factor, noise-floor, full-scale). The die-level measurements are carried out with a dedicated electronic circuit implemented with discrete components, developed during this work.In-plane accelerometers showed static capacitance and resonance frequency in line with theory. They achieved resolution smaller than 8 µg/rtHz and full scale in the order of 160g. These aspects together lead to a dynamic range of more than 145dB (BW=1Hz) for a device with a footprint of only 0.24 mm². This it more than 100 times larger than the DR of consumer device of similar size. These results are achieved while keeping a large bandwidth and working with an open-loop readout.Out-of-plane sensors showed resonance frequency higher than expected due to fabrication tolerances. The devices had both smaller mass and thicker springs explaining the observed mechanical behavior. Despite the loss of scale factor due to the larger resonance frequency, these sensors achieved resolution ranging from 50-80 µg/rtHz. Again, such performance was obtained while keeping large resonance frequency (>8 kHz), small footprint (down to 0.22 mm²) and a potential full-scale of more than 200g. In the future, design corrections and process improvement could lead to device with thicker inertial layer, aligning the performance of out-of-plane sensors to those of in-plane ones and leading to a high-performance 3-axis accelerometer.This type of sensor could address the demand of emerging applications for high-stability, low-noise and large DR accelerometers within consumer footprint.Finally, the proposed technology offers a fabrication platform for inertial MEMS sensors and actuators. New design possibilities and great potentialities have been demonstrated with the first fabricated accelerometers. In the future this new concept could be applied to several other types of MEMS, like gyroscope or micro-mirrors.

This dissertation focuses on one of the most active research areas in the microelectronics industry: Thermo-mechanical reliability of 3-D interconnects containing through-silicon-vias (TSVs). This study constitutes two parts: 1. Thermal stress measurement on TSVs; 2. Analyses on thermo-mechanical reliability of TSVs. In the first part, a metrology for stress measurement of through-silicon-via (TSV) structures was developed using a bending beam technique. The bending curvature induced by the thermal expansion of a periodic array of Cu TSVs was measured during thermal cycles. The stress components in TSV structures were deduced combining the curvature measurement with a finite-element-analysis (FEA). Temperature-dependent thermal stresses in Cu TSVs and in Si matrix were derived. In the second part, the reliability issues induced by the thermal stresses of TSVs were analyzed from several aspects, including the carrier mobility change in transistors, the interfacial delamination of TSVs, and thermal stress interactions between TSVs. Among them, the mobility change in transistors was found to be sensitive to the normal stresses near the Si wafer surface. The surface area of a high mobility change was defined as the keep-out zone (KOZ) for transistors. FEA simulations were carried out to calculate the area of KOZ surrounding TSVs. The area of KOZ was found to be mainly determined by the channel direction of the transistor as a result of anisotropic piezoresistivity effects. FEA simulations also showed that the KOZ can be controlled by TSV geometry, material selection, etc. Interfacial delamination of TSVs was found to be mainly driven by a shear stress concentration at the TSV/Si interface. Crack driving force for TSV delamination was calculated using FEA simulations, which take into account the magnitude of thermal load, TSV geometry, TSV materials, etc. The results provided a design guideline to improve the TSV delamination problem. In the last, the stress interaction among TSV arrays was examined using a bi-TSV model. In the Cartesian coordinate system, thermal stresses can be intensified or suppressed between TSVs, depending on how TSVs are located. Further analyses suggested that the area of KOZ and the TSV-induced Si cracking can both be improved by optimizing the arrangement of the TSV arrays. / text

We report herein on the results of N- and O-alkylation of 5-cyano-2-methylsulfanyl-4(3H)- pyrimidinone with 4-substituted ω-bromoacetophenones. Synthesized N-(phenacyl)-4- pyrimidinones and 4-(phenacyloxy)pyrimidines with suitable substituents in pyrimidine ring are versatile synthons for the preparation of fused pyrimidine heterocycles. Chemical properties of new furo[2,3-d]pyrimidines were investigated. The structure of synthesized compounds was confirmed by data of 1H NMR, IR and UV spectra.

Les dépôts gravitaires carbonatés peuvent former d'importants volumes à la transition plate-forme/bassin; notamment en domaine tropical où les biotopes néritiques carbonatés sont de prolifiques usines à sédiments pouvant être redéposés dans le milieu marin profond adjacent. Ce travail porte sur les dépôts gravitaires carbonatés d'âge Crétacé de la Péninsule du Gargano (SE Italie). Une cartographie géologique détaillée et la modélisation numérique 3D (supportées par un MNT obtenu par LIDAR héliporté) ont été couplées à des analyses sédimentologiques et biostratigraphiques afin de caractériser l'évolution spatiale et temporelle de systèmes de dépôts gravitaires distincts. Les surfaces stratigraphiques 3D ont été restaurées afin d'évaluer l'impact de la paléo-topographie sur la distribution spatiale et la géométrie des corps. La corrélation avec les séries de plate-forme de même âge a permis d'établir les relations entre nature des resédimentations (sables vs. brèches) et le niveau marin. Les données qualitatives et quantitatives d'affleurements ont permis de générer des modèles numériques 3D de lithofacies à partir de méthodes de simulations stochastiques. Enfin, des mesures des paramètres physiques des roches ont été couplées à des analyses pétrographiques afin de d'évaluer l'impact de la fabrique sédimentaire et des types poreux sur les propriétés acoustiques et réservoirs de ces carbonates. Cette approche multidisciplinaire démontre l'importance du couplage de méthodes d'analyses de terrain dites «traditionelles» avec des donnés numériques et la modélisation 3D afin d'améliorer la caractérisation et les modèles des systèmes et réservoirs sédimentaires. / Carbonate gravity flow deposits can form significant volumes at the platform-to-basin transition; especially because tropical shallow-water carbonate environments are major sediment factories, the products of which, early lithified or not, are likely to be redeposited in the adjacent deep marine domain. This work has focused on the Cretaceous resedimented carbonates of the Gargano Peninsula (SE Italy). Extensive geological mapping and 3-D numerical modelling (supported by a LIDAR DEM) were associated to sedimentologic and biostratigraphic analyses to assess the spatial and temporal evolution of distinct deep-water depositional systems. Key 3-D stratigraphic surfaces were restored to investigate the impact of the paleo-topography on the geometry and spatial distribution of the gravity flow deposits. Correlation with the coeval shallow-water platform series has served to assess the relationships between the nature of the resedimented carbonates (i.e. bioclastic sands versus breccias) and sea level. Qualitative and quantitative outcrop data were transferred into 3-D numerical models of lithofacies that were generated at seismic scale with stochastic simulation methods. Finally, petrophysical measurements were coupled with quantitative petrographic analyses to assess the impact of sedimentary fabrics and pore types on acoustic and reservoir properties of carbonate gravity flow deposits. This multidisciplinary approach demonstrates that coupling traditional field work analyses with outcrop numerical data (e.g. LIDAR-derived) and 3-D geological modelling is a relevant method for improving outcrop characterization and conceptual models of sedimentary systems and reservoirs.

Diploma work is focused on computational comparison of con-rod of spark ignition four-cylinder engine with volume 1289 cubic centimeters and con-rod of spark ignition three-cylinder engine with volume 1198 cubic centimeters. 3-D models of inspected con-rods were based on real shape. Force loads effects were established after kinematic and dynamic analysis. Stress analysis was made for these force loads effects in selected cases with help of Finite element method. Two con-rods were compared by using of these results.

The present study deals with the effects of sparsely distributed three-dimensional elements on two-dimensional (2-D) and three-dimensional (3-D) turbulent boundary layers (TBL) such as those that occur on submarines, ship hulls, etc. This study was achieved in three parts: Part 1 dealt with the cylinders when placed individually in the turbulent boundary layers, thereby considering the effect of a single perturbation on the TBL; Part 2 considered the effects when the same individual elements were placed in a sparse and regular distribution, thus studying the response of the flow to a sequence of perturbations; and in Part 3, the distributions were subjected to 3-D turbulent boundary layers, thus examining the effects of streamwise and spanwise pressure gradients on the same perturbed flows as considered in Part 2. The 3-D turbulent boundary layers were generated by an idealized wing-body junction flow. Detailed 3-velocity-component Laser-Doppler Velocimetry (LDV) and other measurements were carried out to understand and describe the rough-wall flow structure. The measurements include mean velocities, turbulence quantities (Reynolds stresses and triple products), skin friction, surface pressure and oil flow visualizations in 2-D and 3-D rough-wall flows for Reynolds numbers, based on momentum thickness, greater than 7000. Very uniform circular cylindrical roughness elements of 0.38mm, 0.76mm and 1.52mm height (k) were used in square and diagonal patterns, yielding six different roughness geometries of rough-wall surface. For the 2-D rough-wall flows, the roughness Reynolds numbers, based on the element height (k) and the friction velocity, range from 26 to 131. Results for the 2-D rough-wall flows reveal that the velocity-defect law is similar for both smooth and rough surfaces, and the semi-logarithmic velocity-distribution curve is shifted by an amount depending on the height of the roughness element, showing that this amount is a function of roughness Reynolds number and the wall geometry. For the 3-D flows, the data show that the surface pressure gradient is not strongly influenced by the roughness elements. In general, for both 2-D and 3-D rough-wall TBL, the differences between the two roughness patterns (straight and diagonal), as regards the mean velocities and the Reynolds stresses, are limited to about 3 roughness element heights from the wall. The study on single elements revealed that the separated shear layers emanating from the top of the elements form a pair of counter rotating vortices that dominate the downstream flow structure. These vortices, termed as the roughness top vortex structure (RTVS), in conjunction with mean flow, forced over and around the elements, are responsible for the production of large Reynolds stresses in the neighborhood of the element height aft of the elements. When these elements are placed in a distribution, the effects of RTVS are not apparent. The roughness elements create a large region of back flow behind them which is continuously replenished by faster moving fluid flowing through the gaps in the rough-wall. The fluid in the back flow region moves upward as low speed ejections where it collides with the inrushing high speed flow, thus, leading to a strong mixing of shear layers. This is responsible for the generation of large levels of turbulent kinetic energy (TKE) in the vicinity of the element height which is transported, primarily, by turbulent diffusion. As regards the 3-D rough-wall TBL, the effect of flow three-dimensionality is seen in the large skewing of the distributions of mean velocities, Reynolds stresses and TKE, aft of the elements. In general, the regions of large TKE production-rates seem to propagate in the direction of the local velocity vector at the element height. The data-sets also enable the extraction of the turbulent flow structure to better describe the flow physics of these rough-wall turbulent boundary layers. / Ph. D.

Collaboration between the playwright, director, designer and technicians is the backbone of theater. Costume designers, after discussion with the director and the other designers, rely on costume technicians to realize their ideas. The technician’s hands then realize the artistic vision. But what would the technicians try if there were no constraints? I am a technician, a craftsperson who is inspired not just by the play, but also by process, by methods. With every new technique I learn, I imagine the new ways it could be put to use, and the objects I would create if time and resources were not a factor. Imagined objects are seldom created. Once made, these objects exist without a performance, without a purpose. A costume not worn is an unfulfilled destiny. But maybe the pieces I want to make can be given a narrative after the act of construction, or during construction. I’m interested in exploring my ability to be a generative artist. How can my inspiration feed back into the theater community? Can a costume technician’s experimentation have a place in creating new theater? My thesis has two components, exploring my two interests. Those two interests are the creation of objects and the creation of a story. The first component, object creation, was an exploratory study of mask and headdress making techniques. I experimented with new techniques, such as 3-D printing, testing the limitations of new technology. The second component, story creation, was a collaborative process. My collaborator, Brian Oglesby, and I worked concurrently. Brian is a playwright, and as he wrote the play, I made the objects. Our processes mirrored each other. The narrative of the play incorporated the masks and headpieces I made. This project created a theater piece based on the experimentation of a costume technician, and presents a new way for future technicians to think about their work and to have their stories told. / text

Na robótica, mapear o ambiente é tarefa importante, porque ela oferece informação para o planejamento e execução de movimentos do robô. Por este motivo, aqui são apresentados estudos que visam a construção de mapas 3-D e técnicas que auxiliam na tarefa de mapeamento. Quando são construídos mapas 3-D, é habilitado para outros pesquisadores e empresas de robótica a desenvolverem trabalhos de análise e planejamento de trajetórias em todos os seis graus de liberdade do corpo rígido que serve para modelar um robô móvel, robô manipulador ou robô móvel manipulador. Com uma representação do ambiente em 3-D, é aumentada a precisão do posicionamento do robô em relação ao ambiente e também o posicionamento de objetos que estão inseridos no campo de atuação do robô. Para solucionar o problema de mapeamento são apresentadas técnicas teóricas e suas aplicações em cada caso estudado. Nos experimentos realizados neste trabalho foi adotada a criação de mapas com grids (malhas) de ocupação. Vale lembrar, no entanto, que a construção de mapas por malhas de ocupação pressupõe o conhecimento do posicionamento do robô no ambiente. Neste trabalho foram conduzidos três experimentos e seus objetivos são redução de dados provenientes de falhas e redundâncias de informação com utilização de técnicas probabilísticas, detecção de movimento através da técnica de extração de fundo e mapeamento 3-D utilizando a técnica de ponto mais próximo. No experimento cujo o objetivo é reduzir os dados, foi possível reduzir para 4,43% a quantidade de pontos necessários para gerar a representação do ambiente com a utilização do algoritmo deste trabalho. O algoritmo de mapeamento 3-D feito com uso de modelos probabilísticos bem estabelecidos e disponíveis na literatura tem como base a probabilidade de eventos independentes e a proposta do trabalho envolvendo probabilidade a posteriori. O experimento de detecção de movimento foi gerado com a utilização da openCV e a tecnologia CUDA e utilizam a técnica do modelo de mistura gaussiana (GMM), foi analisado o tempo de processamento desempenhado por cada implementação e a qualidade do resultado obtido. Para obter uma representação precisa do ambiente foi conduzido o experimento que utiliza técnica iterativa do ponto mais próximo (ICP), para realização foi utilizado o sensor de movimento Kinect e os resultados apresentados não foram satisfatórios devido ao volume de dados adquiridos e a ausência de um sistema de estimativa da localização. / In robotics, map the environment is an important task, because it provides information for planning and executing movements of the robot. For this reason, studies presented here are aimed to build 3-D maps and techniques that aid in the task of mapping. When we build 3-D maps, we enable other researchers and robotics companies to develop analyzes and path planning in all six degrees of freedom rigid body that serves to model a mobile robot, manipulator or mobile robot manipulator.With a representation of the environment in 3-D, we increase the accuracy of the robot positioning in relation to the environment and also the positioning of objects that are inserted into the field of action of the robot. To solve the problem of mapping we presented theoretical techniques and their applications in each case studied.In the experiments in this work we adopted the creation of maps with grids of occupation. However, building grids of occupation assumes knowledge of the position of the robot on the environment.In this work we conducted three experiments and their aims are the reduction of data from failures and redundant information using probabilistic techniques, motion detection by background extraction technique and 3-D mapping technique using the closest point. In the experiment whose goal is to reduce the data has been further reduced to 4.43% the number of points required to generate the representation of the environment with the use of our algorithm.The algorithm of 3-D mapping done with probabilistic models available and well established in the literature is based on the probability of independent events and the proposed work involving the posterior probability.The motion detection experiment was performed with the use of openCV and CUDA technique using the Gaussian mixture model (GMM),and we analyzed the processing time and the quality of each implementation result.For an accurate representation of the environment was conducted the experiment using the technique of iterative closest point (ICP) was used to perform the motion sensor Kinect and the results were not satisfactory due to the volume of data acquired and the absence of a system location estimate.

Gelenkknorpel ist ein gefäßloses, hoch spezialisiertes Gewebe mit nur sehr begrenzter Regenerationsfähigkeit. Entstandene Läsionen werden bei natürlicher Heilung durch min-derwertigen Faserknorpel gefüllt. Ein vielversprechender Therapieansatz kommt aus dem Gebiet des Tissue Engineering. Dabei werden isolierte Chondrozyten in vitro vermehrt und anschließend in den Defekt eingebracht. In den letzten Jahren ist hier die 3 D-Kultivierung in patientenspezifischen Biomaterialien zunehmend in den Fokus der Forschung geraten. Ziel der hier vorgestellten Studie war es, die Tauglichkeit von Vollblut und Blutplasma als Aus-gangsmaterial für MACTs aufgrund makroskopischer Eigenschaften, Zellzahlentwicklung im Konstrukt, Zellvitalität und Syntheseleistung charakteristischer EZM-Marker zu untersuchen. Es wurde für diese Studie Knorpel aus den Fesselgelenken vier geschlachteter Pferde (2-16 Jahre) entnommen, mechanisch zerkleinert und anschließend mit Kollagenase A verdaut. Von einem 6 jährigen klinikeigenen Wallach wurde Vollblut in Citratröhrchen gewonnen, ein Teil zu Plasma weiterverarbeitet und beides bei -80 °C bis zur weiteren Verwendung Schock gefroren. Zur Herstellung der walzenförmigen Konstrukte mit den Maßen 9,6 cm2 x 4,7 mm, wurden 4,5 ml Vollblut bzw. Plasma mit je 3x106 Chondrozyten suspensiert und durch Zuga-be von CaCl2 zur Koagulation gebracht. Der Kultivierungszeitraum betrug 28 Tage in DMEM, angereichert mit 10% allogenem Serum und 1% Antibiotika. Die Konstrukte wurden an den Tagen 1, 14 und 28 auf Zellzahl, -vitalität und mithilfe qRT-PCR auf hyaline Knorpelmarker wie Kollagen Typ II und Aggrekan untersucht. Zudem wurden histologische und immunhisto-chemische Präparate der Konstrukte angefertigt. Die Zellvitalität betrug sowohl in den VB-, als auch in den BP-Konstrukten ≥95% bei steigen-der Zellzahl (bis zu 5x106 im Vollblutkonstrukt). Die MACTs beider Ausgangsmaterialien schrumpften auf eine Größe von 2 cm² x 2 mm. Histologisch konnten in beiden Konstruktar-ten mit der Alzianblaufärbung sGAG belegt werden. Darüber hinaus wurde der sGAG Gehalt mit dem DMMB Assay quantitativ ermittelt. Aggrekan, C-4-S, C-6-S und COMP wurden als wichtige Bestandteile der EZM immunhistochemisch angefärbt und waren ebenfalls in beiden Konstruktarten nachweisbar. Mit der qRT PCR konnte die Genexpression von Aggrekan, Kol II und Kol I über den zeitlichen Verlauf ermittelt werden. Es stellte sich heraus, dass sich sowohl die Genexpression von Aggrekan als auch die von Kol II, den beiden Indikatorprotei-nen EZMs des hyalinen Gelenkknorpels über den Kultivierungszeitraum absenkten. Dies deutet auf eine Dedifferenzierung der Chondrozyten hin. Die Expression von Kol I dagegen stieg um ein Vielfaches an. Auch das kennzeichnet eine Dedifferenzierung. Zieht man ande-re Studien heran, so ist die festgestellte Umstellung der Genexpression aber vergleichsweise niedrig, eine Dedifferenzierung weg vom chondrogenen Phänotyp in der hier vorliegenden Arbeit also weniger stark ausgebildet. Biokompatibilität mit und Abbaubarkeit im Empfängerorganismus konnten in dieser in vitro Untersuchung nicht evaluiert werden. Zusammenfassend kann man festhalten, dass VB und BP als Ausgangsbiomaterialien zur Herstellung von MACT geeignet sind. Inwieweit es gelingen wird, den chondrogenen Phäno-typ beispielweise durch mechanische Stimulation der eingesäten Zellen stärker zu erhalten, muss in folgenden Studien geklärt werden. Ebenfalls weiterer Forschungsbedarf ist bei den Eigenschaften Elastizität und Steifheit gegeben. Grundsätzlich gilt, dass MACTs auf VB- und BP-Basis einen einfachen, kostengünstigen und patientenspezifisch herstellbaren Therapie-ansatz für die Behandlung von Knorpeldefekten darstellen. / Articular cartilage is a vessel-free, highly specialized tissue with only very limited regenera-tive capacity. Resulting lesions are filled with inferior fibrocartilage by natural healing. A promising therapeutic approach comes from the field of tissue engineering. Therefore iso-lated chondrocytes are expanded in vitro and then placed into the defect. In the last few years the 3-D culturing in patient-specific biomaterials has come increasingly into the focus of research. Purpose of the present study was to evaluate the suitability of whole blood and blood plasma as a basic material for MACT based on macroscopic properties, development of cell number in the construct, cell viability and synthetic performance of characteristic markers. For this study cartilage from the four fetlock joints of slaughtered horses (2-16 years) were removed, crushed mechanically and then digested with Collagenase A. Whole blood was obtained in citrate tubes of a 6 year old gelding owned by the clinic, finished part to both plasma and shock frozen at -80°C until further use. To prepare the cylindrical constructs, measuring 9.6 cm2 x 4.7 mm, 4.5 ml of whole blood or plasma, each with 3x106 chondro-cytes were suspended and coagulated by the addition of CaCl2. The cultivation period was 28 days in DMEM supplemented with 10% allogenic serum and 1% antibiotics. The con-structs were evaluated on days 1, 14 and 28 on cell number, viability, and using qRT-PCR examination for hyaline cartilage markers such as collagen type II and aggrecan. In addition, histological and immunohistochemical preparations of the constructs were made. The cell vitality was in the WB, as well as in the BP constructs ≥ 95% with increasing cell number (up to 5x106 in whole blood construct). The MACTs of both basic materials shrink to a size of 2 cm x 2 mm². Histologically sGAG could be verified in both construct species by Alzianblau staining. In addition, the GAG content was determined with the DMMB assay quantitatively. Aggrecan, chondroitin-4-sulphate, chondroitin-6-sulphate and COMP as major components of the ECM were stained immunohistochemically and were also detectable in both types of constructs. Aggrecan, collagen II and collagen I were determined on the time course by qRT PCR gene expression. It turned out that the gene expressions of both, aggre-can and of collagen II, the two ECM protein indicators of hyaline cartilage lowered over the cultivation period. This indicates a dedifferentiation of chondrocytes. The expression of colla-gen I on the other hand increased to a multiple, also featuring a dedifferentiation. If one approached other studies, the observed change in gene expression is comparatively low. In the present work the dedifferentiation from the chondrogenic phenotype is less distinctive. Biocompatibility and biodegradability in the recipient organism could not be evaluated in this in vitro investigation. In summary, one should notice that VB and BP are suitable basic materials for the production of MACT. It has to be clarified by following studies, to what extent the chondrogenic pheno-type can be strengthened, for example by mechanical stimulation of cells sown. Also further research is needed on the given properties, e.g. elasticity and stiffness. Generally MACT based on WB and BP- is a simple, inexpensive and patient-specific produced therapeutic approach for the treatment of cartilage defects.