Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2015-12-21T08:12:55Z No. of bitstreams: 2 Dissertação - Laura Garcia Vila - 2015.pdf: 2794084 bytes, checksum: ded55da11b6147f4147cce56cf18ec53 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-12-21T08:16:07Z (GMT) No. of bitstreams: 2 Dissertação - Laura Garcia Vila - 2015.pdf: 2794084 bytes, checksum: ded55da11b6147f4147cce56cf18ec53 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2015-12-21T08:16:07Z (GMT). No. of bitstreams: 2 Dissertação - Laura Garcia Vila - 2015.pdf: 2794084 bytes, checksum: ded55da11b6147f4147cce56cf18ec53 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-09-18 / Restraint induced stress in wild birds can be life threatening. Research for alternatives that minimize those risks iswell justified, sedation beinga suitable option. In the present study midazolam sedation was tested in two species of wild birds. Twenty-one blue-and-yellow macaws were randomly assigned in two groups, one receiving saline solution (control group) and the other approximately 7.5 mg/kg of midazolam (midazolam group) via an intranasal spray, in two different moments with a washout period of two months. Fourteen greater rheas were submitted to a similar protocol; however, the midazolam dosage was 1 mg/kg via an intramuscular route. After 10 min following sedative/saline application, physical exam (heart rate, respiratory rate and temperature) and venous blood collectionwere performed. Blood samples were processed for hemogasometry (only with macaws), hematology and biochemistry analyses. In macaws, midazolam application produced sedation; lower values for physical exam parameters and significantly (p<0.05) lower lactate, base excess and chloride concentrations, while pCO2, ctCO2 and HCO3 values were higher when compared with control group. In rheas, the only significant difference was the respiratory rate, being higher in the midazolam group. In macaws midazolam attenuated metabolic acidosis derived from restraint, showing a positive effect on stress reduction, proving an effective and safe alternative. In response to midazolam rheas showed inconstant reaction depending among individuals, with no reliable effect on stress restraint reduction. / O estresse produzido pela contenção em aves silvestres pode resultar em graves complicações. A procura por alternativas que minimizem tais riscos está plenamente justificada, sendo uma delas, a sedação. No presente estudo a sedação com midazolam foi testada em duas espécies de aves silvestres. Vinte e uma araras canindé foram randomizadas e divididas em dois grupos, um recebeu solução salina (grupo controle) e o outro aproximadamente 7,5 mg/kg de midazolam (grupo midazolam) via spray intranasal, em dois períodos de tempo diferentes, separados por dois meses. Catorze emas foram submetidas a um protocolo semelhante, porém a dose do midazolam foi 1 mg/kg,via intramuscular. Após 10 min do momento da aplicação do sedativo/salina foi feita a avaliação física (frequência cardíaca, frequência respiratória e temperatura) e a colheitado sangue por punção venosa. Foi realizada hemogasometria (somente nas araras), hematologia e bioquímica das amostras sanguíneas. Nas araras, a aplicação do midazolam produziu sedação, valores mais baixos para os parâmetros de avaliação física e concentrações significativamente (p<0.05) mais baixas de lactato, excesso de base e cloro enquanto que os valores de pCO2, ctCO2 e HCO3 foram mais elevados em comparação ao grupo controle. Nas emas, a única diferença significativa observada foi na frequência respiratória, sendo maior no grupo midazolam. Nas araras, o midazolam atenuou a acidose metabólica derivada da contenção, apresentando efeito na redução do estresse, mostrando-se uma alternativa segura e efetiva. A resposta ao midazolam nas emas variou em função do indivíduo,sem efeito consistente na redução do estresse.

A natureza heterogênea da Leucemia Mielóide Aguda (LMA) tornou-se um desafio para o sucesso da quimioterapia convencional com o agente Citarabina (Ara-C), especialmente em leucemias com prognóstico desfavorável, como aquelas portadoras do rearranjo MLL. Visto que as células de LMA-MLL são consideradas sensíveis ao Ara-C quando comparadas às leucemias que não apresentam o rearranjo, mas a recaída à doença é frequente, a presente tese propôs estudar a relação entre características biológicas relacionadas às bases da resistêmcia ao Ara-C em LMA-MLL. A abordagem proposta foi a seleção de subpopulações de linhagens celulares portadoras do rearranjo MLL submetidas ao tratamento em longo prazo com Ara-C, comparando-as com as linhagens não expostas à droga. As células foram caracterizadas quanto: 1) ao potencial proliferativo na presença ou ausência de Ara-C; 2) a distribuição das células no ciclo celular; 3) a distribuição de marcadores clássicos de superfície de células-tronco hematopoiéticas, CD34 e CD38; e 4) o perfil de expressão global dos RNAs transcritos. O tratamento em longo prazo selecionou células mais resistentes ao Ara-C que as células parentais. Além disso, quanto ao ciclo celular, as células selecionadas com Ara-C apresentaram apoptose reduzida (fase sub-G1), acúmulo na fase de síntese (fase S) e aumento da capacidade proliferativa após reexposição à droga (fase G2-M). Quanto à análise de marcadores de células-tronco hematopoiéticas, observou-se que após o tratamento em longo com Ara-C, uma das linhagens celulares apresentou distribuição bimodal do marcador CD38. Quando separadas por sorting em citometria de fluxo, observou-se que as subpopulações com níveis distintos de expressão de CD38, denominadas MV-4-11 CD38High e MV-4-11 CD38Low apresentaram resposta distinta ao tratamento com Ara-C. Quando avaliadas quanto ao perfil global de expressão gênica, constatou-se que MV-4-11 CD38High eram mais semelhantes às células parentais, e que MV-4-11 CD38Low formavam um grupo isolado, distinto das outras duas populações celulares. A análise de ontologia gênica (GO) evidenciou que entre as categorias mais representativas de processos biológicos estavam atividades associadas à capacidade proliferativa, ao desenvolvimento e a resposta a estímulos. As análises de agrupamentos hierárquicos mostraram que: 1) o cluster de genes do desenvolvimento HOXA estava mais expresso nas células MV-4-11 CD38Low do que em MV-4-11 CD38High, que apresentaram expressão mais elevada do cluster HOXB; 2) o gene HOX mais diferencialmente expresso foi HOXA13, associado na literatura com prognóstico desfavorável em outros tipos de câncer; 3) dos genes associados a resposta a estímulos, o único relacionado à via de metabolização do Ara-C diferencialmente expresso entre as linhagens foi NME1; 4) aqueles que participam das vias de reparo de pareamento incorreto, reparo por excisão de bases e por excisão de nucleotídeos encontraram-se mais expressos nas células MV-4-11 CD38High que em MV-4-11 CD38Low. Além disso, diversas quinases dependentes de ciclinas (CDKs) também estiveram diferencialmente expressas entre MV-4-11 CD38High e MV-4-11 CD38Low. Sugere-se por fim, que o modelo in vitro proposto neste estudo para simular a situação de resistência ao Ara-C em subpopulações de LMA-MLL, demonstrou que os mecanismos de resposta à Citarabina nesta doença, vão além de alterações na detoxificação e metabolização da droga, e parecem mais associados a vantagens proliferativas e do desenvolvimento das células leucêmicas. Estas vias devem ser exploradas como alvos potenciais na terapia combinada ao Ara-C. / The heterogeneity of Acute Myeloid Leukemia (AML) became a challenge for the success of the conventional chemotherapy agent Cytarabine (Ara-C), especially in leukemias with poor prognosis, as those harboring MLL rearrangement. Since AML-MLL cells are considered sensitive to Ara-C when compared with leukemias that do not carry the rearrangement, but relapse is frequent, the present dissertation proposed to study the relationship between biological characteristics related to the basis of chemoresistance to Ara-C in AML-MLL. We proposed an approach based on the selection of subpopulations of cell lines bearing MLL rearrangement submitted to the long-term treatment with Ara-C, comparing them with the cell lines that were not previously exposed to the drug. The cells were characterized according to: 1) the proliferative potential in the presence and absence of Ara-C; 2) the distribution of the cells in the cell cycle; 3) distribution of hematopoietic stem cell classic surface markers, CD34 and CD38; and, 4) global expression profile of transcribed RNAs. The long-term treatment selected cells that are more resistant to Ara-C than the cells that were not previously treated (parental cells). Besides, according to cell cycle, the cells selected by Ara-C treatment present decreased apoptosis (sub-G1 phase), accumulation in the synthesis phase (S-phase) and increase in the proliferative capability after re-exposition to the drug (G2-M phase). Regarding the hematopoietic stem cell markers, we observed that after Ara-C long-term treatment, one of the cell lines exhibited a bimodal distribution of the CD38 marker. When sorted by flow cytometry, we observed that both subpopulations with distinct levels of CD38 expression, called MV-4-11 CD38High and MV-4-11 CD38Low also showed distinct response to Ara-C. When evaluated regarding to their global gene expression profiles, we verified that MV-4-11 CD38High were more closely related to the parental cells, and MV-4-11 CD38Low made up an isolated group, distinct of the other cell populations. Gene ontology (GO) analysis revealed that among the most representative categories of biological processes, activities associated with proliferative capability, development and response to stimuli were included. The hierarchical clustering analysis showed that: 1) the cluster HOXA of genes of development was more expressed in the MV-4-11 CD38Low than in the MV-4-11 CD38High cells, that presented increased expression of HOXB cluster; 2) the most differentially expressed HOX gene was HOXA13, which according to the literature is associated with poor prognosis in other types of cancer; 3) among the genes associated with response to stimuli, the only one related to Ara-C-metabolizing pathway that was differentially expressed between the cell lines was NME1; 4) those genes that take part in the mismatch repair, base excision repair and nucleotide excision repair pathways were more expressed in the MV-4-11 CD38High than in the MV-4-11 CD38Low cells. Additionally, several cyclin-dependent kinases (CDKs) were also differentially expressed between MV-4-11 CD38High and MV-4-11 CD38Low. Finally, we suggest that the in vitro model proposed in this study to mimic the situation of chemoresistance to Ara-C in subpopulations of AML-MLL, showed that the mechanisms of Ara-C response in this disease, go beyond changes in drug detoxification and metabolization, and seem more associated to proliferative and development advantages of the leukemic cells. These pathways should be explored as potential targets to Ara-C combination therapies.

Sammanfattning Titel: Norrlandsboomen - en kvalitativ studie om platsmarknadsföring för att attrahera nya invånare. Nivå: Examensarbete på grundnivå (kandidatexamen) i ämnet företagsekonomi Författare: Julia Karlernäs och Simon Ivarsson Handledare: Katarina Arbin Datum: 2024 -januari Syfte: Denna uppsats syftar till att beskriva hur arbetet med platsmarknadsföring sker i samverkan mellan olika aktörer för att attrahera nya invånare till en ny geografisk plats Metod: Studien har använt sig av en kvalitativ metod med en abduktiv forskningsansats och grundar sig i insamlad empirisk data från sex intervjuer. Resultat och slutsats: Studien visar att de aktiviteter som sker i platsmarknadsföringen bland annat är marknadskommunikation av platsen via olika digitala plattformar, utveckling av samhället efter identifierade målgruppers efterfrågan samt arbete med värdskap och social inkludering för nyblivna invånare. Resurser som används är ekonomiska resurser, som hos kommunen är skattemedel och från H2 Green Steel kommer från investerare. Andra resurser är humankapital, organisatoriska och -relationella resurser. Huvudaktörer som är involverade är Boden kommun, turistföreningen Boden Turism, H2 Green Steel och Bodens invånare. Examensarbetets bidrag: Kandidatuppsatsen ger insikter i hur platsmarknadsföringen i en kommun går till för att attrahera nya invånare. Genom att identifiera aktiviteterna som utförs, resurserna som finns och vilka aktörerna är, klargör vi hur en kommun arbetar med platsmarknadsföring och vilka roller och uppgifter de olika organisationerna har. Vår studie bidrar även med kunskap om hur samarbetet och samverkan ser ut mellan de olika aktörerna. Förslag till fortsatt forskning: Förslag till vidare forskning är att göra en större studie där fler perspektiv i platsmarknadsföringen studeras. Vidare föreslår vi att ytterligare studier bör fokusera på hur man kan mäta vilken effekt platsmarknadsföringen har på beslutet att flytta till en ny kommun. Nyckelord: Platsmarknadsföring, platsvarumärke, ARA-modell, nyindustrialisering, kompetensbrist / Abstract Title: Norrlandsboomen - a qualitative study of place marketing in attracting new residents. Level: Student thesis, Bachelor Degree in Business Administration Supervisor: Katarina Arbin Date: 2024 - january Aim: The aim of this study is to describe how place marketing occurs in collaboration between actors to attract new residents to a new geographic location. Method: The study used a qualitative method with an abductive research approach and is based on collected empirical data from six interviews. Results and conclusions: The study shows that activities occurring in place marketing are, among others, marketing communication of the place through digital platforms, development of the municipality based on the demands of identified target groups and the work with hosting and social inclusion of new residents. Resources used are financial resources, which at the municipality comes from tax money and within H2 Green Steel from investors. Other resources are human capital, organizational and -relational resources. The main actors involved are Boden municipality, the tourist association Boden Turism, H2 Green Steel and the residents of Boden. Contribution of the thesis: The thesis provides insights into how place marketing in a municipality takes place to attract new residents. By identifying the activities that are carried out, the resources that are used and who the actors are, we clarify how a municipality works with place marketing and what roles and tasks the various actors have. Our study also contributes to knowledge about how the cooperation and collaboration takes place between the various actors. Suggestions for future research: Suggestions for future research is to do a bigger study where more perspectives in place marketing is taken into account. Furthermore, we suggest that additional studies should focus on how to measure the effect that place marketing has in the decision about moving to a new municipality. Key words: Place marketing, place brand, ARA-model, new industrialization, skill shortage

Det finns idag en rad exempel på branscher där ett nytt företag förändrat hur kärnverksamheten genomförs. Detta har skett genom implementation av teknologi i en traditionell bransch. Mycket har skrivits om hur disruptiva företag verkar, men det finns bristande kunskap kring hur etablerade företag påverkas, och anpassar sin verksamhet. En framträdande skillnad mellan disruptiva och etablerade företag är att det etablerade företaget har ett befintligt nätverk att stödja sig mot. Syftet med studien är att öka förståelsen för hur företags nätverk påverkas av digitalisering inom industrin till följd av en disruptiv innovatörs etablering i industrin. Studien applicerar ARA-modellen, ekosystem och konceptet tjänstefiering som teoretiskt ramverk. En abduktiv forskningsansats appliceras, med en kvalitativ metod och fallstudie som forskningsstrategi. Fordonsindustrin väljs ut som en lämplig bransch att genomföra studien i, och Volvo som fallföretag. Det empiriska materialet består av sexton semistrukturerade intervjuer och sekundärdata. Intervjuerna har genomförts dels hos fallföretaget, dels med informanter i fallföretagets nätverk, vilket analyseras med hjälp av tematisk analys. Studiens resultat identifierade tre teman: digitalisering, samarbete och en fysisk och digital syntes. Studiens slutsatser är att konkurrensfördelar erhålls genom samarbete, där gemensamma resurser leder till ökad effektivitet. Konfigureringen av nya aktörsroller inom företaget möjliggör denna process. Digitalisering och tjänstefiering har påvisats vara ömsesidigt förstärkande processer, som sammantaget främjar implementationen av ett ekosystem. Inom hela processen är den disruptiva innovatören en drivande part. / There are several examples today of industries where a new company has changed how the core business is conducted. This has consistently occurred through implementation of technology in an otherwise traditional industry. Much has been written on how disruptive companies operate, but there is a lack of knowledge concerning how established companies are affected and adapt their operations. A prominent difference between the disruptive and the established companies is that the established companies have an existing network to rely on. The purpose of this study is to increase the understanding of how a company’s network is affected by digitalization within the industry as a result of a disruptive innovator’s establishment in the industry. The study applies the ARA-model, ecosystem and the concept of servitization as a theoretical framework. An abductive research approach is applied, with a qualitative method and case study as its research strategy. The automotive industry is chosen as a suitable industry for the study, and Volvo is chosen as the case company. The empirical material consists of sixteen semi-structured interviews and secondary data. The interviews were conducted both at the case company and with informants in the case company’s network, which is analyzed using thematic analysis. The study’s findings identified three themes: digitalization, collaboration, and a physical and digital synthesis. The conclusions of the study is that competitive advantages are acquired through collaboration, where shared resources lead to an increased efficiency. The configuration of new actor roles makes this process possible. Digitalization and servitization has shown to be mutually reinforcing processes that collectively promote the implementation of an ecosystem. Through the entire process, the disruptive innovator is a driving force.

Essential fatty acids are necessary for growth, survival, and development of larval fishes, but there is limited information on the essential fatty acid requirements of larval southern flounder (Paralichthys lethostigma). The objectives of this study were to elucidate connections between dietary supply of docosahexaenoic acid (DHA) and arachidonic acid (ARA) and deposited fatty acids in the head or body, and then link diet and stored fatty acids in the head or body with larval quality traits. From 4-15 days posthatch (dph), southern flounder larvae were fed rotifers enriched with four different combinations of DHA-rich Algamac 3050 and ARA-rich Algamac ARA. Fatty acid concentrations in the head and body were measured at 15 dph, and relationships between fatty acids in head or body and in the diet were determined. Larval quality traits, including specific growth rate (SGR), survival, and eight behavioral performance variables were measured. Results showed that concentrations of DHA and ARA in the head and in the body were correlated with concentrations of DHA and ARA in the diet. Growth rate did not vary among the four diets, but survival was positively correlated with the amount of lipid in the diet. Responsiveness to a visual stimulus was positively correlated with the concentration of DHA in the diet, the ratio DHA:EPA in the head, and total energy content of the diet. Turning rate during routine swimming was correlated with body DHA. This study demonstrates the influence of DHA content, total lipid content, and energy levels in the diet of southern flounder and provides a foundation for future studies examining causal factors of recruitment variability or larviculture production success. / text

Dentre as fases do ciclo de vida do Gerenciamento de Processos de Negócios (BPM - Business Process Management), a fase de diagnóstico de processos pode facilitar a mudança de modo a fundamentar futuros esforços de aumento de produtividade, padronização da execução do trabalho e criação de uma rotina de trabalho mais eficiente. Como técnica de diagnóstico, a ARA (Árvore da Realidade Atual) é mais ampla pois demonstra a visão geral da situação da empresa. A pergunta de pesquisa que se pretende responder com este trabalho é: como o diagnóstico da Árvore da Realidade Atual pode promover melhorias em uma Unidade de Pesquisa Clínica (UPC)? O objetivo é analisar o relacionamento entre a aplicação do diagnóstico da ARA e as melhorias realizadas em uma UPC. Para tanto, foi utilizado como método um estudo de caso em uma UPC que utilizou ARA como técnica de diagnóstico precursora da implementação de melhorias organizacionais. Nos resultados deste estudo é apresentado o diagnóstico da ARA em uma UPC e são descritas as melhorias que ocorreram na organização a partir da aplicação desta técnica. Além disso, são identificados quais fatores de sucesso do BPM contribuíram para a aplicação da ARA. Conclui-se que a utilização da ARA como técnica de diagnóstico, além de fornecer uma análise que contempla as necessidades reais da organização, pode ser relacionada com o sucesso da implementação de melhorias. Este estudo deve fornecer subsídios aos gestores de UPCs em relação à efetividade das práticas administrativas passíveis a serem adotadas, além de ser importante para pesquisadores que atuam com diagnóstico de processos e BPM ao apresentar a efetividade da utilização da ARA como técnica e relacionar as melhorias obtidas com os fatores críticos de BPM. / Among the phases of the Business Process Management (BPM) lifecycle, the process diagnostics phase can facilitate the change in order to support future efforts to increase productivity, standardize the execution of work and create a more efficient work routine. As a diagnostic technique, CRT (Current Reality Tree) is broader because it demonstrates the overall picture of the company\'s situation. The research question that intends to answer with this work is: how the diagnosis of the Current Reality Tree can promote improvements in a Clinical Research Unit (CRU)? The objective is to analyze the relationship between the application of CTR diagnosis and the improvements performed in a UPC. For that, a case study was used as a method in a UPC that used CRT as diagnostic tool for the implementation of organizational improvements. The results of this study present the diagnosis of CRT in a CRU and describe the improvements that occurred in the organization from the application of this technique. In addition, it is identified which BPM success factors contributed to the application of ARA. It is concluded that the use of ARA as a diagnostic technique, besides providing an analysis that considers the real needs of the organization, can be related to the success of the implementation of improvements. This study should provide support to UPC managers concerning to the effectiveness of the administrative practices that may be adopted, and it is important for researchers working with process and BPM diagnoses to introduce the effectiveness of the use of ARA as a technique and to relate the improvements obtained with the critical BPM factors.

A realidade andina, e particularmente a realidade agraria andina, desde a migração ilegal e intrusa do k´arusuyu (europeu), se tem manifestado distante às ações, políticas ou visões de desenvolvimento pensadas fora de seu espaço, aspecto que derivou na busca do próprio desenvolvimento: Sumak Kawsay, esse próprio, contextualizado no Etnodesenvolvimento. O estudo argumentou respostas a esse real diferente, aprofundado desde outro olhar, o pensar próprio, desde Guaman Poma de Ayala (1615) e Santa Cruz Pachacuti (1613), ambos, plasmam nas sus imagens a relação entre seres de distintos espaços, uma relação Ser-Ser, o não ser não, elemento que pervive desde uma complexidade no real atual. Nesse contexto, para entender a realidade agraria andina, se considerou dois espaços de realidades não isoladas das dinâmicas totais: os distritos de Orurillo e Sicuani, no Qollasuyo; utilizando como método o Teqsimuyuq Kawsaynin – Hinantin Suyupi (TK-HS): convivência com tudo, em todo lugar. Os resultados mostram que, as realidades andinas perviven com outra própria visão do eruivalente ao “desenvolvimento”, refletida nas tecnologias, saberes, ciencias, pensares, infraestruturas, etc., que vem desde antes e até o Tahuantinsuyo, transitando logo a o Andino, caminhando dentro do bom governo como em tempos do Inca, ao não governo do k´arusuyu, seguido logo pelo musoqk´ara, até a atualidade. Nesse entender, a realidade agraria andina reflete continuação do Tahuantinsuyo, desde seus próprios pensares, o próprio termo andino é referente. O próprio desenvolvimento desde um pensar andino, é a relacao de convivencia com o agro, parte da co-existência, relacao total não encaixada no ritual ou cerimonial só. O agro não é atividade, recursos, etapa a superar, nem é inferior, é um igual com que se convive, assim se chega à convivencia equilibrada, Sumak Kawsay, o desejo do desenvolvimento sustentável: emergencia do “desenvolvimento” ocidental. Nesse entender o agro andino representa o desejo u objetivo da agricultura sustentavel, sendo que, esta ultima requer reconstruir suas bases epistemológicas se quer converter-se em realidade. / The Andean reality, and particularly the Andean agrarian reality, since the illegal and intrusive migration of the k'arusuyu (European), has manifested itself far from the actions, policies or visions of development thought outside of its space, an aspect that led to the search of the own development: Sumak Kawsay, this very one, contextualized in Ethnodevelopment. Guaman Poma de Ayala (1615) and Santa Cruz Pachacuti (1613), both of them, portray in their images the relation between beings of different spaces, a relation Being-Being, not being not exist, element that survives from a complexity in the current real. In this context, to understand the Andean agrarian reality, two spaces of realities not isolated from the total dynamics were considered: the districts of Orurillo and Sicuani, in Qollasuyo; using as method the Teqsimuyuq Kawsaynin - Hinantin Suyupi (TK-HS): coexistence with everything, everywhere. The results, show that the Andean realities survive with another vision of the erudent to the "development", reflected in the technologies, knowledge, sciences, thought, infrastructures, etc., that comes from before and until Tahuantinsuyo. Transiting soon to the Andean, walking within the good government as in times of the Inca; to the non-government of k'arusuyu, followed later by the musoqk'ara, until the present time. In this understanding, the Andean agrarian reality reflects the continuation of the Tahuantinsuyo, from its own thoughts; the Andean term itself is referent. The development itself from an Andean thinking, is the relation of coexistence with the agro, part of the co-existence, total relation not fit in the ritual or only ceremonial. Agro is not activity, resources, stage to be overcome, nor is it inferior, it is an equal with which it coexists, thus comes to a balanced coexistence, Sumak Kawsay, the desire for sustainable development: emergence of Western "development". In this understanding, the Andean agriculture represents the goal of sustainable agriculture, and the latter requires reconstructing its epistemological basis if it is to become a reality.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-04-05T20:08:44Z No. of bitstreams: 1 2016 - Diego Renan Sobreiro Falc?o Paim.pdf: 1171234 bytes, checksum: 293452c2fece2086e9010a78bb8b4db1 (MD5) / Made available in DSpace on 2017-04-05T20:08:44Z (GMT). No. of bitstreams: 1 2016 - Diego Renan Sobreiro Falc?o Paim.pdf: 1171234 bytes, checksum: 293452c2fece2086e9010a78bb8b4db1 (MD5) Previous issue date: 2016-03-07 / CAPES / This study aimed to obtain a product in probiotic powder to ju?ara base, whose palm is being threatened with extinction due to indiscriminate practice of palm heart extraction and, in this sense, add value to this fruit is a proposal in an attempt to preserve the species. Initially, ju?ara pulp added probiotic microorganism Bifidobacterium spp. lactis was subjected to drying by spray drying (spray drying), which promoted a microencapsulation. In this first step, differet types and combinations of carriers agents were evaluated, totalizing four formulations: maltodextrin, maltodextrin + inulin (1:1), maltodextrin + oligofructose (1:1) and maltodextrin + inulin + oligofructose (2:1:1). The samples produced with different formulations were characterized in relation to the viable cell count, phenolics, anthocyanins, ABTS + antiradical activity, density, porosity, particle size and hygroscopicity. The samples produced with maltodextrin + inulin (1: 1) showed the best results, being selected for further stability study. In this study, samples were also produced by freeze drying, in order to compare the processes. The powders produced by the two processes were stored at different temperatures (7 and 35 ? C) and evaluated periodically for 60 days. Results showed that the samples stored at 7?C showed microorganism cell count higher than 107 CFU/g after 60 days of storage, being considered as probiotic products, whereas samples stored at 35?C showed cell counts lower than 104 CFU/g after 30 days. The samples produced by freeze drying showed higher viable cell counts compared to those produced by spray drying, after 30 days of storage. Regarding the phenolic content and antirradical activity, the samples showed a very similar behavior to each other. / Este trabalho teve como objetivo a obten??o de um produto em p? probi?tico ? base de ju?ara, cuja palmeira vem sendo amea?ada de extin??o devido ? pr?tica indiscriminada do extrativismo do palmito e, nesse sentido, agregar valor a esse fruto ? uma proposta, na tentativa de preservar a esp?cie. Inicialmente, a polpa de ju?ara adicionada do microrganismo probi?tico Bifidobacterium spp. lactis foi submetida ao processo de secagem por atomiza??o (spray drying), que promoveu sua microencapsula??o. Nesta primeira etapa, foram avaliados diferentes tipos e combina??es de agentes carreadores, totalizando quatro formula??es: maltodextrina, maltodextrina + inulina (1:1), maltodextrina + oligofrutose (1:1) e maltodextrina + inulina + oligofrutose (2:1:1). As amostras produzidas com as diferentes formula??es foram caracterizadas em rela??o ? contagem de microrganismos vi?veis, fen?licos totais, antocianinas, atividade antirradical ABTS+, densidade, porosidade, higroscopicidade e tamanho de part?culas. As amostras produzidas com maltodextrina + inulina (1:1) foram as que apresentaram os melhores resultados, sendo selecionadas para o posterior estudo de estabilidade. Nesse estudo, as amostras foram produzidas tamb?m por liofiliza??o, a fim de se comparar os processos. O produto em p? produzido pelos dois processos foi estocado a diferentes temperaturas (7 e 35?C) e avaliado periodicamente, durante 60 dias. Os resultados mostraram que as amostras estocadas a 7?C apresentaram contagem de microrganismos superior a 107 UFC/g ap?s 60 dias de estocagem, podendo ser consideradas probi?ticas, enquanto as amostras armazenadas a 35?C apresentaram contagem inferior a 104 UFC/g a partir de 30 dias. As amostras produzidas por liofiliza??o apresentaram maior contagem quando comparadas ?s produzidas por atomiza??o, a partir de 30 dias de estocagem. Em rela??o ao teor de fen?licos e ? atividade antirradical, as amostras apresentaram comportamentos muito semelhantes entre si.

acase@tulane.edu

Les allergies alimentaires à l'arachide et au lait posent un problème majeur de santé publique, en particulier chez les enfants. Nous avons développé un modèle cellulaire de dégranulation, afin d'analyser si la liaison d'un allergène à ses IgE spécifiques, mesurée in vitro, a bien la capacité de déclencher la phase effectrice de la réaction allergique. Ce modèle repose sur l'utilisation de la lignée cellulaire RBL SX-38 développée par JP Kinet, mastocytes de rat immortalisés et modifiés pour exprimer le récepteur de haute affinité aux IgE humaines. Le développement de ce modèle pour son utilisation en microplaques de 96 puits a consisté notamment en l'optimisation de différents paramètres critiques pour la réalisation des 2 phases du test : la phase de sensibilisation des cellules par les IgE de patients allergiques et la phase de déclenchement par les allergènes auxquels le patient est sensibilisé. Ce modèle a permis de montrer l'importance des caséines et des albumines 2S (Ara h 2 et Ara h 6) dans cette étape clé de la réaction allergique au lait et à l'arachide, confirmant les observations sérologiques et cliniques disponibles. L'effet de traitements thermiques sur l'allergénicité d'un allergène majeur de l'arachide (Ara h 1), a été évalué dans le cadre du programme européen EuroPrevall. Les résultats obtenus montrent la diminution de la réactivité d'Ara h 1 après chauffage de la protéine isolée en solution. Par contre, la même protéine préparée et purifiée à partir d'arachide grillée présente une forte réactivité. Ces résultats suggèrent qu'un traitement à température élevée de la graine entraine une augmentation de l'allergénicité de Ara h 1 du fait de ses interactions avec les autres constituants de la graine. Le modèle cellulaire de dégranulation développé apparaît être un outil pertinent pour l'étude de la fonctionnalité biologique de l'interaction allergène-IgE, permettant une meilleure compréhension de la relation entre la structure d'une protéine alimentaire et son allergénicité, voire une évaluation du risque allergique des aliments ne nécessitant pas d'essais cliniques systématiques.