On March 23, 2010, President Barack Obama signed the Patient Protection and Affordable Care Act (PPACA) into law. This reform, it is argued, is projected to increase insurance coverage of pre-existing conditions, to expand access to insurance for more than 30 million Americans, and to increase estimated National medical spending while lowering projected Medicare spending. This thesis sought to investigate and analyze the perceptions of senior citizens in Central Florida about PPACA and their perceived effects on the healthcare quality provided to them under this law. Four sections of PPACA bill, thought to specifically pertain to the elderly, were selected for this study; respondents were asked their opinions regarding PPACA's aspects of: (1) the reform on preventive healthcare services; (2) Medicare Part D (prescription drugs); (3) Medicare; and (4) Medicaid. This thesis employed both qualitative and quantitative methodologies; data were collected and analyzed with findings presented and discussed.

La pseudouridylation, l'isomérisation du résidu urine (U) en pseudouridine ([PSI]) est la modification post-transcriptionnelle la plus fréquemment retrouvée dans les ARN. Elle est catalysée par une enzyme ARN:PSI-synthase. Chez les archées et les eucaryotes, cette activité est également portée par des particules ribonucléoprotéiques à boîtes H/ACA (RNP H/ACA). Chez les archées, le complexe comprend quatre protéines invariables dont l'ARN:PSI-synthase aCBF5 et trois protéines partenaires L7Ae, aNOP10 et aGAR1, ainsi qu'un ARN guide qui cible par appariement de bases la position de l'uridine à modifier de l'ARN substrat. Le rôle des partenaires a pu être identifié par des analyses structure-fonction basées sur des approches biochimiques, biophysiques et radiocristallographiques. Au cours de ce travail, nous avons démontré l'existence de disparités fonctionnelles entre les ARN guides d'un même organisme, et l'importance de l'interaction entre L7Ae et aNOP10 pour le positionnement correct de l'ARN substrat. Nous avons testé in vitro l'assemblage et l'activité de particules reconstituées en présence d'ARN guides non conventionnels. L'étude sur la dynamique de l'ARN substrat lors de la pseudouridylation a également été abordée et a permis de déterminer que aGAR1 n'était pas nécessaire pour le mécanisme de turnover de la particule, que la température jouait un rôle crucial pour cette activité, et que la nature du nucléotide cible ainsi que la longueur de l'ARN substrat étaient des éléments importants pour la sélection de cet ARN. Nous avons également mis au point une nouvelle technique basée sur le phénomène de FRET permettant de suivre l'association de l'ARN substrat à la RNP H/ACA / Pseudouridylation reaction that consists in the isomerization of uridines (U) into pseudouridines (PSI) is the most frequent post-transcriptional modification found in RNAs. It is catalyzed by enzymes with RNA:PSI-synthase activity. In Archaea and Eukarya, ribonucleoprotein particles, the so-called box H/ACA RNPs, possess such activity. In Archaea, the box H/ACA complex comprises four invariable proteins namely the RNA:PSI-synthase aCBF5 and three protein partners L7Ae, aNOP10 and aGAR1, and specific to each RNP, an RNA acting as a guide to secure by base pairing the RNA substrate and define the position to be modify. During these last years, several crystal structures of components of archaeal H/ACA RNP and fully assembled RNP have been resolved. Complementary biochemical and biophysical studies allowed detailed structure-function analyses to identify the role of the different components. During this work we identified functional differences between two RNA guides expressed in the same archaea, and demonstrated that the interaction between L7Ae and aNOP10 is important for a correct positioning of substrate RNA. We also tested in vitro the assembly and activity of RNP reconstituted on H/ACA-like guide RNAs. We investigated dynamics of substrate RNA during the pseudouridylation. We found that aGAR1 was not necessary for the turnover of the particle, that the temperature was crucial for such activity, and that the chemical structure of the targeted residue and length of the substrate RNA were important determinants for substrate selectivity. Finally, we have also developed a new technic based on FRET adapted to monitor binding of the susbtrate RNA to the box H/ACA RNP enzyme

Made available in DSpace on 2016-01-26T12:51:19Z (GMT). No. of bitstreams: 1 jorgeadasdib_tese.pdf: 343099 bytes, checksum: ab3f9830cf7060129a9dcec26d66b83c (MD5) Previous issue date: 2008-06-06 / No previous study has addressed the question of prognostic determinants for patients with Chagas? cardiomyopathy at the terminal stage listed for heart transplantation. Casuistic and Method: All patients listed for heart transplantation at our institution from August, 2000 to March, 2005 were considered for the study. Patients removed from the waiting list for clinical status improvement were excluded from the investigation. Patients were followed until death, cardiac transplantation or the end of the study period. Cardiac transplant recipients were censored at the time of transplantation. No patient was lost to follow up. A Cox regression hazards model was used to establish independent predictors of all-cause mortality. Variables previously demonstrated to predict mortality in either Chagas or non-Chagas? disease heart failure were entered the univariate analysis. Separate analyses were performed for Chagas and non-Chagas? disease patients. Results: Median follow up was 32 (15,121) days in Chagas disease and 79 (14,151) days in non-Chagas? disease patients. In Chagas disease patients, the hemodynamic instability (p=0.01; hazard ratio=0,077, 95% confidence interval, 0.01 to 0.58) as well as the transpulmonary gradient (p=0.02; hazard ratio=1.15, 95% confidence interval, 1.02 to 1.30) were retained as independent predictors of all-cause mortality. Serum sodium levels (p=0.002; hazard ratio=0.81; 95% confidence interval, 0.71 to 0.93) was independent predictor of all-cause mortality for non-Chagas? disease patients. Conclusion: The hemodynamic instability and transpulmonary gradient were independent predictors of all-cause mortality for Chagas? disease patients listed for heart transplantation. A larger, prospective cohort study is needed to validate our findings. / At? agora nenhum estudo preocupou-se em estabelecer determinantes progn?sticos para pacientes com insufici?ncia card?aca cr?nica terminal secund?ria ? cardiomiopatia da doen?a de Chagas na fila de espera para transplante card?aco. Casu?stica e M?todo: Todos os pacientes alocados em fila de espera de transplante card?aco em nossa institui??o, de agosto de 2000 a mar?o de 2005, foram inicialmente considerados para o estudo. Os pacientes que foram removidos da lista de espera em virtude de melhora no estado cl?nico foram retirados do estudo. Os pacientes foram acompanhados at? a morte, transplante card?aco ou a data final estipulada para o estudo. Os pacientes receptores de transplante de cora??o foram retirados do estudo na data em que o ato operat?rio ocorreu. N?o se perdeu contato com os pacientes durante o acompanhamento cl?nico enquanto na fila de espera de transplante card?aco. O modelo de an?lise de riscos proporcionais de Cox foi utilizado para se estabelecer vari?veis de predi??o independentes de mortalidade geral. As vari?veis que eram capazes de predizer mortalidade geral em pacientes com insufici?ncia card?aca cr?nica secund?ria ? cardiomiopatia da doen?a de Chagas ou a outras cardiomiopatias foram utilizadas no modelo univariado. An?lises univariadas foram feitas nos pacientes chag?sicos e n?o chag?sicos separadamente. Resultados: A mediana do tempo de acompanhamento cl?nico foi 32 (15, 121) dias nos pacientes chag?sicos e 79 (14, 151) dias nos pacientes n?o chag?sicos. Nos pacientes chag?sicos, a instabilidade hemodin?mica (p=0,01; raz?o de risco=0,077, intervalo de confian?a de 95% entre 0,01 e 0,58) e o gradiente transpulmonar (p=0,02; raz?o de risco =1,15, intervalo de confian?a de 95% entre 1,02 e 1,30) foram as vari?veis de predi??o independentes de mortalidade geral. Os n?veis s?ricos de s?dio (p=0,002; raz?o de risco =0,81; intervalo de confian?a de 95% entre 0,71 e 0,93) foi a vari?vel de predi??o independente para os pacientes n?o chag?sicos na fila de espera para transplante card?aco. Conclus?es: A instabilidade hemodin?mica e o gradiente transpulmonar foram preditores independentes de mortalidade geral em pacientes com insufici?ncia card?aca cr?nica secund?ria ? cardiomiopatia da doen?a de Chagas na lista de espera para transplante card?aco. Um estudo prospectivo de coorte longitudinal ? necess?rio para validar os resultados obtidos nesta investiga??o.

Submitted by PPG Engenharia El?trica (engenharia.pg.eletrica@pucrs.br) on 2018-10-29T13:30:23Z No. of bitstreams: 1 TIAGO BENETTI_DIS.pdf: 5038519 bytes, checksum: 95fa8d1b367b574eee27e772a55a9a49 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-10-30T17:21:55Z (GMT) No. of bitstreams: 1 TIAGO BENETTI_DIS.pdf: 5038519 bytes, checksum: 95fa8d1b367b574eee27e772a55a9a49 (MD5) / Made available in DSpace on 2018-10-30T17:27:25Z (GMT). No. of bitstreams: 1 TIAGO BENETTI_DIS.pdf: 5038519 bytes, checksum: 95fa8d1b367b574eee27e772a55a9a49 (MD5) Previous issue date: 2018-08-31 / Heart rate monitoring using Photoplethysmography (PPG) signals acquired from the individuals pulse has become popular due to emergence of numerous low cost wearable devices. However, monitoring during physical activities has obstacles because of the influence of motion artifacts in PPG signals. The objective of this work is to introduce a new algorithm capable of removing motion artifacts and estimating heart rate from pulse PPG signals. Normalized Least Mean Square (NLMS) and Recursive Least Squares (RLS) algorithms are proposed for an adaptive filtering structure that uses acceleration signals as reference to remove motion artifacts. The algorithm uses the Periodogram of the filtered signals to extract their heart rates, which will be used together with a PPG Signal Quality Index to feed the input of a Kalman Filter. Specific heuristics and the Quality Index collaborate so that the Kalman filter provides a heart rate estimate with high accuracy and robustness to measurement uncertainties. The algorithm was validated from the heart rate obtained from Electrocardiography signals and the proposed method with the RLS algorithm presented the best results with an absolute mean error of 1.54 beats per minute (bpm) and standard deviation of 0.62 bpm, recorded for 12 individuals performing a running activity on a treadmill with varying speeds. The results make the performance of the algorithm comparable and even better than several recently developed methods in this field. In addition, the algorithm presented a low computational cost and suitable to the time interval in which the heart rate estimate is performed. Thus, it is expected that this algorithm will improve the obtaining of heart rate in currently available wearable devices. / O monitoramento da frequ?ncia card?aca utilizando sinais de Fotopletismografia ou PPG (do ingl?s, Photopletismography) adquiridos do pulso de indiv?duos tem se popularizado devido ao surgimento de in?meros dispositivos wearable de baixo custo. No entanto, o monitoramento durante atividades f?sicas tem dificuldades em raz?o da influ?ncia de artefatos de movimento nos sinais de PPG. O objetivo deste trabalho ? introduzir um novo algoritmo capaz de remover artefatos de movimento e estimar a frequ?ncia card?aca de sinais de PPG de pulso. Os algoritmos do M?nimo Quadrado M?dio Normalizado ou NLMS (do ingl?s, Normalized Least Mean Square) e de M?nimos Quadrados Recursivos ou RLS (do ingl?s, Recursive Least Squares) s?o propostos para uma estrutura de filtragem adaptativa que utiliza sinais de acelera??o como refer?ncia para remover os artefatos de movimento. O algoritmo utiliza o Periodograma dos sinais filtrados para extrair suas frequ?ncias card?acas, que ser?o utilizadas juntamente com um ?ndice de Qualidade do Sinal de PPG para alimentar a entrada de um Filtro de Kalman. Heur?sticas espec?ficas e o ?ndice de Qualidade colaboram para que filtro de Kalman forne?a uma estimativa da frequ?ncia card?aca com alta acur?cia e robustez a incertezas de medi??o. O algoritmo foi validado a partir da frequ?ncia card?aca obtida de sinais de Eletrocardiografia e o m?todo proposto com o algoritmo RLS apresentou os melhores resultados com um erro m?dio absoluto de 1,54 batimentos por minuto (bpm) e desvio padr?o de 0,62 bpm, registrados para 12 indiv?duos realizando uma atividade de corrida em uma esteira com velocidades variadas. Os resultados tornam o desempenho do algoritmo compar?vel e at? mesmo melhor que v?rios m?todos desenvolvidos recentemente neste campo. Al?m disso, o algoritmo apresentou um custo computacional baixo e adequado ao intervalo de tempo em que a estimativa da frequ?ncia card?aca ? realizada. Dessa forma, espera-se que este algoritmo melhore a obten??o da frequ?ncia card?aca em dispositivos wearable atualmente dispon?veis.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2018-09-12T18:08:44Z No. of bitstreams: 1 2017 - Julia Dias Villas Boas.pdf: 1378660 bytes, checksum: 2a1537e1b92056b0327c8e50b73feb34 (MD5) / Made available in DSpace on 2018-09-12T18:08:44Z (GMT). No. of bitstreams: 1 2017 - Julia Dias Villas Boas.pdf: 1378660 bytes, checksum: 2a1537e1b92056b0327c8e50b73feb34 (MD5) Previous issue date: 2017-04-28 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / The horse has a natural predisposition for the sport, however, its use in competitions can result in stress related problems that impair its sporting performance and especially its health. In this way it is fundamental not only to understand how the different risk and resilience factors to different stressors influence the response to stress, but also to develop strategies that can prevent or minimize the deleterious effects of stress. In this sense, acupuncture is an ancient technique of Traditional Chinese Medicine that has been used in the treatment and prevention of stress-related diseases. The present study proposed the use of two models of stress: one physical (physical exercise) and another psychological (startle model) to verify the reactivity to the stress of athletes horses. In addition, it was also evaluated if horses of different sporting modalities present different psychological stress responses and if acupuncture can alter the responses to physical stress. In the experiment 1, 16 Thoroughbred race horses were submitted to a exercise in the field of high intensity and short duration (12 m / s, 4 min). The RR intervals for analysis of the Heart Rate Variability were acquired through the Polar Equine ? heart rate monitor and blood samples were collected before and immediately after 2h, 4h, 6h, and 24h after exercise. The exercise promoted autonomic alterations in the sympatho-vagal balance since it significantly increased the low frequency component (LF), the heart rate and the LF / HF ratio, and decreased the high frequency component (HF) (p <0.01). There was an increase in hematocrit, plasma proteins, glucose and plasma lactate immediately after exercise (p <0.001). There was an increase (p <0.01) in serum cortisol values after 30 minutes, returning to baseline values after 60 min. However, no significant difference was observed in plasma cytokines IL-1? and IL-6 between moments after exercise and baseline. In experiment 2, horses of the experimental group 1 after exercise were randomly divided into two groups: CTL (C2): Control (without manipulation) and ACUP (C2)): animals submitted to weekly sessions of acupuncture at points VG1, C7, VG20 and B52 for 10 weeks. After the treatment period the animals repeated the same exercise and the same parameters were analyzed. Acupuncture reduced the LF / HF ratio, promoting a faster recovery of the animals, showing no influence on the other parameters analyzed. In the experiment 3, 24 equines were used, from three equestrian modes: Polo (PSI) (n = 9), Dressage (Brazilian Horse Riding) (n = 6) and Endurance (n=6) were subjected to the experimental model of startling through the abrupt opening of an umbrella. The results showed that endurance horses presented a significantly less intense startle-induced autonomic response than Polo and Dressage horses (lower LF / HF ratio at the time of the jump), paradoxically Enduro horses have cortisol levels in response in response to the startle than Polo horses. However, there was no difference between the modalities in the behavioral response after the startle, and Polo horses had significantly higher baseline levels of cortisol than the other modalities and did not change their cortisol levels in response to stress. Thus, we can conclude that 1) the exercise in the field of high intensity and short duration promoted adaptive changes characteristic of stress, being able to be used in studies of reactivity to stress in race horses; 2) acupuncture has a modulating effect on the stress-induced autonomic response in athletic horses, and 3) the equestrian modalities of Dressage, Polo and Endurance present different stress reactivity / O cavalo tem uma predisposi??o natural para o esporte, no entanto, o seu uso em competi??es pode resultar em problemas relacionados ao estresse que prejudicam seu desempenho esportivo e principalmente a sua sa?de. Desta forma ? fundamental n?o apenas entender como os diferentes fatores de risco e de resili?ncia a diferentes estressores influenciam a resposta ao estresse, como tamb?m desenvolver estrat?gias que possam prevenir ou minimizar os efeitos delet?rios do estresse. Neste sentido a acupuntura ? uma t?cnica milenar da Medicina Tradicional Chinesa que tem sido utilizada no tratamento e preven??es de doen?as relacionadas ao estresse. O presente estudo prop?s o uso de dois modelos de estresse: um f?sico (exerc?cio f?sico) e outro psicol?gico (modelo de sobressalto) para verificar a reatividade ao estresse de cavalos atletas. Al?m disso, tamb?m foi avaliado se cavalos de diferentes modalidades esportivas apresentam respostas ao estresse de psicol?gico distintas e se acupuntura pode alterar as respostas ao estresse f?sico. No experimento 1, 16 equinos de corrida da ra?a Puro Sangue Ingl?s foram submetidos ao exerc?cio a campo de alta intensidade e curta dura??o (12 m/s, 4min). Os intervalos RR para an?lise da Variabilidade da Frequencia Card?aca foram adquiridos atrav?s do frequenc?metro card?aco Polar Equine? e as amostras de sangue foram coletadas antes e, imediatamente, 2h, 4h, 6h, e 24h ap?s o exerc?cio. O exerc?cio promoveu altera??es auton?micas no balan?o simpato-vagal uma vez que aumentou significativamente o componente de baixa frequ?ncia (LF), a frequ?ncia card?aca e a raz?o LF/HF e diminuiu o componente de alta frequ?ncia (HF) (p < 0.01). Houve aumento do hemat?crito, das prote?nas plasm?ticas, glicose e lactato plasm?tico imediatamente ap?s o exerc?cio (p < 0.001). Houve aumento (p<0.01) nos valores s?ricos de cortisol ap?s 30 minutos, retornando aos valores basais ap?s 60min. No entanto, n?o foi observado diferen?a significativa nas citocinas plasm?ticas IL-1? e IL-6 entre os momentos ap?s exerc?cio e o momento basal. No experimento 2: os equinos do experimento 1 ap?s o exerc?cio foram aleatoriamente divididos em dois grupos: CTL (C2): Controle (sem manipula??o) e ACUP (C2)ACUP (C2): animais submetidos a sess?es semanais de acupuntura nos pontos VG1, C7, VG20 e B52 durante 10 semanas. Ap?s o per?odo de tratamento os animais repetiram o mesmo exerc?cio e foram analisados os mesmos par?metros. A acupuntura reduziu a raz?o LF/HF, promovendo uma recupera??o mais r?pida dos animais n?o apresentando influ?ncia nos demais par?metros analisados. No experimento 3, foram utilizados 24 equinos, pertencentes a tr?s modalidades equetres: P?lo (ra?a PSI) (n=9), Adestramento (ra?a Brasileiro de Hipismo) (n=6) e Enduro (Puro Sangue ?rabe) (n=9) submetidos ao modelo experimental de sobressalto atrav?s da abertura abrupta de um guarda-chuva. Os resultados mostraram que cavalos de enduro apresentaram resposta auton?mica induzida pelo sobressalto significativamente menos intensa que cavalos de Polo e Adestramento (menor raz?o LF/HF no momento do sobressalto), paradoxalmente cavalos de Enduro possuem n?veis de cortisol em resposta ao sobressalto mais altos que cavalos de Polo. N?o houve diferen?a entre as modalidades na resposta comportamental ap?s o sobressalto, no entanto cavalos de P?lo apresentam n?veis basais de cortisol significativamente mais altos que as demais modalidades e n?o variaram seus n?veis de cortisol em resposta ao estresse. Dessa forma, podemos concluir que 1) o exerc?cio a campo de alta intensidade e curta dura??o promoveu altera??es adaptativas caracter?stica de estresse, podendo ser utilizado em estudos de reatividade ao estresse em cavalos de corrida; 2) a acupuntura tem um efeito modulador da resposta auton?mica induzida pelo estresse em cavalos atletas e 3) as modalidades equestres de Adestramento, Polo e enduro apresentam reatividade ao estresse distintas

Black women in the United States have a high prevalence of hypertension and suffer the most complications of cardiovascular disease. Black women, though aware of the dangers associated with hypertension, have limited opportunity to access health care and or change their lifestyles. The purpose of this quantitative cross-sectional study was to test if there was a significant difference in hypertension awareness, health care access/use, and lifestyle modifications in Black women prior to and post implementation of The Patient Protection and Affordable Care Act, as compared to women of other races. The behavior modification theory guided this study. Secondary data from the National Health Interview Survey for the years 2009 to 2013 for women ages 20 - 65 were analyzed using logistic regression analysis. According to the study results, there was no association (p values > 0.05) among variables age, education, income, length of employment, and hypertension awareness, health care access/use, and life style modification among Black women in the United States, as compared to women of other races. The findings from this study may allow researchers and policy makers to develop more culturally significant health services for Black women. These findings could create positive social change by targeting programs that promote hypertension awareness leading to effective lifestyle changes in Black women.

Ideal magnetohydrodynamics (MHD) simulations are known to have problems in satisfying the solenoidal constraint (i.e. the divergence of magnetic field should be equal to zero, $ ablacdotvec{B} = 0$). The simulations become unstable unless specific measures have been taken. In this thesis, a solenoidal constraint satisfying technique that allows discrete satisfaction of the solenoidal constraint up to the machine accuracy is presented and validated with a variety of test cases. Due to its inspiration from Chorin's artificial compressibility method developed for incompressible CFD applications, the technique was named as extit{artificial compressibility analogy (ACA)} approach. It is demonstrated that ACA is a purely hyperbolic, stable and consistent technique, which is moreover easy to implement. Unlike some other techniques, it does not pose any problems of the sort that $ ablacdotvec{B}$ errors accumulate in the vicinity of the stagnant regions of flow. With these crucial properties, ACA is thought to be a remedy to the drawbacks of the most commonly used solenoidal constraint satisfying techniques in the literature namely: Incorrect shock capturing and poor performance of the convective stabilization mechanism in regions of stagnant flow for Powell's source term method; exceedingly complex implementation for constrained transport technique due to the staggered grid representation; computationally expensive nature due to the necessity of a Poisson solver combined with hyperbolic/elliptic numerical methods for classical projection schemes. In the first chapter of the thesis, general background knowledge is given about plasmas, MHD and its history, a certain class of upwind finite volume methods, namely Riemann solvers, and their applications in MHD, the definition, constituents, formation mechanisms and effects of space weather and some of the space missions that are and will be performed in its prediction. Secondly, detailed analysis of the compressible ideal MHD equations is given in the form of the derivation of the equations, their dimensionless numbers which will be of use to specify the flows in the following chapters, and finally, the presentation of the MHD waves and discontinuities, which indicates the complexity of the system of ideal MHD equations and therefore their further numerical analysis. The next discussion is about the main subject of the thesis, namely the solenoidal constraint satisfying techniques. First of all, the definition and significance of the solenoidal constraint is given. Afterwards, the most common solenoidal constraint satisfying techniques in the literature are reviewed along with their abovementioned drawbacks. Moreover, particular emphasis is given to the Powell's source term approach which was also implemented in the upwind finite volume MHD solver developed. In addition, the hyperbolic divergence cleaning technique is presented in detail together with the resemblance and differences between it and ACA. Some other solenoidal constraint satisfying techniques are briefly mentioned at this stage. After these, ACA is presented in the following way: The point of inspiration, which is the analogy made with Chorin's artificial compressibility method developed for incompressible CFD, the introduction of the modified system of ideal MHD equations due to ACA, the derivation of the wave equation governing the propagation of $ ablacdotvec{B}$ errors and the analytical consistency proof. Having finished the core discussion of the thesis, the solver developed and its constituents are given in the fourth chapter. Furthermore, a brief overview of the platform into which this solver was implemented, namely COOLFluiD, is also given at this point. Afterwards, a thorough numerical verification of the ACA approach has been made on an increasingly complex suite of test cases. The results obtained with ACA and Powell's source term implementations are given in order to numerically analyse and verify ACA and compare the two methods and validate them with the results from literature. The sixth chapter is devoted to further validation of ACA performed with a variety of more advanced space weather-related simulations. In this chapter, also the $vec{B}_{ extrm{0}} + vec{B}_{ extrm{1}}$ splitting technique used to treat planetary magnetosphere is presented along with its application to ACA and Powell's source term approaches. This technique is utilized in obtaining the solar wind/Earth's magnetosphere interaction results and is based on suppressing the direct inclusion of the Earth's magnetic field, which is a dipole field, in the solution variables. In this way, problems are avoided with the energy equation that could arise from the drastic change of the ratio of the dipole field and the variable field computed by the solver (i.e. $frac{lvertvec{B}_{ extrm{0}}lvert}{lvertvec{B}_{ extrm{1}}lvert}$) in the computational domain. Finally, conclusions and future perspectives related to the material presented in the thesis are put forward.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-04-25T14:57:15Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) lais_matos_oliveira.pdf: 1897599 bytes, checksum: f8cca6f71386c22ad68bc755f7803402 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-05-16T19:49:29Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) lais_matos_oliveira.pdf: 1897599 bytes, checksum: f8cca6f71386c22ad68bc755f7803402 (MD5) / Made available in DSpace on 2017-05-16T19:49:29Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) lais_matos_oliveira.pdf: 1897599 bytes, checksum: f8cca6f71386c22ad68bc755f7803402 (MD5) Previous issue date: 2016 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Objetivou-se com este estudo avaliar as caracter?sticas fisiol?gicas de diferentes grupos gen?ticos mantidos no cerrado. Foram avaliados 48 bovinos de corte criados em sistema precoce para abate aos 24 meses, sendo seis machos e seis f?meas de cada grupo gen?tico (Nelore, ? Nelore x ?Caracu, ? Caracu x ? Nelore, ? Angus x ? Nelore x ?Caracu). Os par?metros fisiol?gicos taxa de suda??o (TS), frequ?ncia card?aca (FC), frequ?ncia respirat?ria (FR), temperatura retal (TR), temperatura da superf?cie corporal (TSC) foram obtidos entre 07:00 e 12:00h. Foram avaliados o comprimento, n?mero de pelos por unidade de ?rea, densidade de massa dos pelos e di?metro m?dio dos pelos. A colora??o do pelo e pele foram avaliadas por meio da Tabela Padr?o e do color?metro MniScan XE Plus. O delineamento experimental foi o inteiramente casualizado em esquema fatorial com seis repeti??es. Foi utilizado o procedimento GLM do SAS, adotando-se para compara??o de m?dias o teste t com n?vel de signific?ncia de 5%. O NEL apresentou maior FR seguido pelo ?NCAR. A FC foi maior no ver?o. A TS foi maior no ver?o em rela??o ao outono. A TR foi maior no ?NCAR, seguido pelo ?CAR, sendo a menor TR observada no NEL e TRI. Houve correla??o positiva entre a temperatura do olho e a temperatura retal. Os pelos do dorso e garupa foram mais compridos no outono, e, em rela??o ao sexo, maiores nas f?meas. O comprimento dos pelos na garupa foi maior no TRI, seguido pelo ?CAR, NEL e ?NCAR. O di?metro dos pelo do dorso foi maior para o ?NCAR, seguido pelo ?CAR, TRI e NEL. O n?mero de pelos na garupa foi maior n?mero no ver?o. Os pelos do dorso foram mais inclinados no ?NCAR, seguidos pelo ?CAR, NEL e TRI. No ver?o, os pelos do dorso permaneceram mais inclinados, e, em rela??o ao sexo, os machos tiveram maior inclina??o no dorso e garupa. A densidade dos pelos no dorso e garupa foi maior no outono. Os pelos da garupa foram mais densos no ?NCAR, seguidos do ?CAR, TRI e NEL. A EC no dorso foi maior no TRI, seguida pelo NEL, ?CAR e ?NCAR. Entre os sexos a EC foi maior nas f?meas. A EC na garupa foi maior no TRI, seguida pelo ?CAR, NEL e ?NCAR. Os pelos tanto do dorso quanto da garupa foram mais claros no NEL. Os pelos mais escuros no dorso foram observados no TRI e na garupa no ?CAR. O grupo gen?tico que demonstrou maior adapta??o ? cria??o no Cerrado foi o NEL seguido do ?NCAR. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016. / The objective with this study was to evaluate the physiological characteristics of different genetic groups maintained in the savannah. Thirty-eight beef cattle raised in a system to be slaughter at 24 months of age were evaluated, with six males and six females of each genetic group (Nellore; ? Nellore x ? Caracu; ? Caracu x ? Nellore; and ? Angus x ? Nellore x ? Caracu). Sweating rate (SR), heart rate (HR), respiratory rate (RR), rectal temperature (RT) and body surface temperature (BST) were obtained between 07:00 and 12:00. Length, number of furs per unit area, mass density of the fur, and the average furs diameter were assessed. Fur and skin coloring were evaluated using the Standard Table and the MniScan XE Plus colorimeter. The experimental design was the completely randomized in a factorial scheme with six replicates. The GLM procedure of SAS was used, adopting the t test for mean comparison with significance level of 5%. The NEL presented higher RR followed by ? NCAR. The HR was higher in the summer. The SR was greater in the summer compared with the autumn. The RT was higher in the ?NCAR, followed by ?CAR, with the lower RT observed in NEL and TRI. There was a positive correlation between eye temperature and rectal temperature. The furs of the dorsum and rump were longer in the autumn, and, in relation to sex, larger in females. The fur length on the rump was longer in TRI, followed by ?CAR, NEL and ?NCAR. The fur diameter on the dorsum was larger for ?NCAR, followed by ?CAR, TRI and NEL. The number of furs on the rump was higher in the summer. The furs on the dorsum were more inclined in ?NCAR, followed by ?CAR, NEL, and TRI. In the summer, the furs on the dorsum remained more leaned, and, in relation to sex, the males have a higher inclination on the dorsum and rump. Fur density on the dorsum and rump was higher in autumn. The furs of the rump were denser in ?NCAR, followed by ?CAR, TRI, and NEL. The EC on the dorsum was higher in TRI, followed by NEL, ? CAR, and ?NCAR. Among the sexes, the EC was higher in females. The EC on the rump was higher in TRI, followed by ? CAR, NEL and ?NCAR. The furs on both dorsum and rump were clearer in the NEL. The darker furs on the dorsum were observed on TRI and on the rump on ? CAR. The genetic group that demonstrated the greatest adaptation to the raising in the savannah was NEL followed by ?NCAR.

Made available in DSpace on 2014-12-17T14:44:18Z (GMT). No. of bitstreams: 1 LuizFFJ_DISSERT.pdf: 561001 bytes, checksum: d3e0298e76c38d08d3b0c195db8c91b5 (MD5) Previous issue date: 2014-04-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The complex human behavior related to exercise involves cognitive, physical and emotional processing. The recent theories about exercise s intensity regulation have highlighted the role played by psychophysics aspects in controlling exercise s intensity. In this regard, recent evidences have shown that there is variability in human capacity in perceiving interoceptives clues. Thus, subjects more sensitive show higher physiological arousal to physical and/or emotional stress, and sensations with higher intensity. In fact, studies have evidenced that interoceptive feedback modifies behavior in exercise with free load. However, exercise recommendations are based in a constant load standard. Therefore, we aimed to analyze the influence of interoceptive sensibility on psychophysics responses during dynamic exercise performed with constant load. Twenty-four adult males were allocated into two groups accordingly with their interoceptive sensibility: high sensibility (n=11) and low sensibility (13). They underwent to an incremental test (IT) and then randomly to two sections of moderate and severe exercise intensity for 20 minutes. Heart rate (HR), rating of perceived exertion (RPE), affective feelings (AF), alert state (AS), and percentage of associative thoughts were collect during exercise. A two-way ANOVA with repeated measures was used to assess differences between psychophysics responses. There were differences between group in RPE, AF, and AS in moderate intensity. There was no difference in any measure in severe intensity. We conclude that subjects with high interoceptive sensibility feel dynamic moderate exercise more intense than the subjecs with low interoceptive sensibility / INTRODU??O: O complexo comportamento humano de realizar exerc?cio f?sico envolve processamento cognitivo, f?sico e emocional. As recentes teorias sobre fadiga e reposta afetivas ao exerc?cio f?sico atribuem importante papel na regula??o da intensidade do esfor?o aos fatores interoceptivos. Al?m disso, Evid?ncias recentes tem apontado haver variabilidade na capacidade humana em perceber esses est?mulos interoceptivos. Dessa forma, pessoas mais sens?veis apresentam excita??o fisiol?gica mais acentuada ao estresse f?sico e/ou emocional, e maior intensifica??o dessas sensa??es. Adicionalmente, estudos tem relatado que feedback interoceptivo diminui a intensidade do esfor?o exercida, e que pessoas com maior sensibilidade interoceptiva controlam melhor a intensidade de esfor?o em tarefas com ritmo livre. Contudo, os modelos de prescri??o de exerc?cio f?sico din?mico apresentam padr?o de intensidade de esfor?o fechado, e n?o ? conhecida as repostas psicofisiol?gicas entre os grupos de variabilidade interoceptiva durante a realiza??o de exerc?cio f?sico. OBJETIVO: O presente estudo verificou a influ?ncia da sensibilidade interoceptiva sobre as repostas psicofisiol?gicas em exerc?cio din?mico com carga constante. M?TODOS: A amostra foi composta por 24 homens jovens adultos, insufientemente ativos, que foram designados a dois grupos de acordo com seus n?veis de sensibilidade interoceptiva: Alta sensibilidade (n=11) e Baixa sensibilidade (n=13). Todos foram submetidos a um teste incremental (TI) em cicloerg?metro para identifica??o do limiar de variabilidade da frequ?ncia card?aca (LiVFC) e da pot?ncia pico (PP). Posteriormente, os sujeitos foram submetidos (em ordem aleat?ria e cruzada) a dois protocolos experimentais de exerc?cio f?sico em cicloerg?metro durante 20 minutos com intensidade correspondente a 20% abaixo e 20% acima LiVFC. Durante os protocolos experimentais as respostas de Frequ?ncia Card?aca (FC), Percep??o Subjetiva de Esfor?o (PSE), Val?ncia Afetiva (VA), Estado de Alerta e N?vel de pensamento Dissociativo-Associativo ao exerc?cio f?sico foram registradas. O teste de Shapiro-Wilk e as an?lises da m?dia, assimetria e curtose verificaram a normalidade de distribui??o dos dados. O Teste de Mauchly verificou a esfericidade dos dados de medidas repetidas. O Teste t para amostra independente e o Teste de Mann Whitney verificaram as igualdade dos dados descritivos entre os grupos. A ANOVA two away (grupo x momentos) para medidas repetidas verificou as poss?veis diferen?as entre as respostas psicofisiol?gicas. RESULTADOS: Houve diferen?a estatisticamente significante entre os grupos na PSE (F(3,36)=18,50, p=0,000), VA (F(3,36)=4,14, p=0,01) e no Estado de Alerta (F(3,36)=4,03, p=0,01) no exerc?cio realizado em intensidade correspondente a 80% LiVFC. Na intensidade correspondente a 120% LiVFC n?o houve diferen?a entre os grupos, somente entre os momentos em cada grupo. CONCLUS?O: A sensibilidade interoceptiva modifica as respostas psicofisiol?gicas durante a realiza??o de exerc?cio f?sico din?mica com intensidade moderada (abaixo do LiVFC)

Made available in DSpace on 2014-12-17T15:16:06Z (GMT). No. of bitstreams: 1 SheylaTSL.pdf: 1123530 bytes, checksum: 9d193a5e8ae6cbd93482dabd85a41d69 (MD5) Previous issue date: 2009-07-30 / Introduction: The ability to walk is impaired in obese by anthropometric factors (BMI and height), musculoskeletal pain and level of inactivity. Little is known about the influence of body adiposity and the acute response of the cardiovascular system during whole the 6-minute walk test (6mWT). Objective: To evaluate the effect of anthropometric measures (BMI and WHR waist-to-hip ratio), the effort heart and inactivity in ability to walk the morbidly obese. Materials and Methods: a total 36 morbidly obese (36.23 + 11.82 years old, BMI 49.16 kg/m2) were recruited from outpatient department of treatment of obesity and bariatric surgery in University Hospital Onofre Lopes and anthropometric measurements of obesity (BMI and WHR), pulmonary function, pattern habitual physical activity (Baecke Questionnaire) and walking capacity (6mWT). The patient was checking to measure: heart rate (HR), breathing frequency (BF), peripheral oxygen saturation, level of perceived exertion, systemic arterial pressure and duplo-produto (DP), moreover the average speed development and total distance walking. The data were analysed between gender and pattern of body adiposity, measuring the behavior minute by minute of walking. The Pearson and Spearmam correlation coefficients were calculated, and stepwise multiple Regression examined the predictors of walking capacity. All analyses were performed en software Statistic 6.0. Results: 20 obese patients had abdominal adiposity (WHR = 1.01), waist circumference was 135.8 cm in women (25) and 139.8 cm in men (10). Walked to the end of 6mWT 412.43 m, with no differences between gender and adiposity. The total distance walked by obesity alone was explained by BMI (45%), HR in the sixth minute (43%), the Baecke (24%) and fatigue (-23%). 88.6% of obese (31) performed the test above 60% of maximal HR, while the peak HR achieved at 5-minute of 6mWT. Systemic arterial pressure and DP rised after walking, but with no differences between gender and adiposity. Conclusion: The walk of obese didn?t suffers influence of gender or the pattern of body adiposity. The final distance walked is attributed to excess body weight, stress heart, the feeling of effort required by physical activity and level of sedentary to obese. With a minute of walking, the obeses achieved a range of intensity cardiovascular trainning / Introdu??o: A capacidade de caminhar dos obesos ? prejudicada pelo ?ndice de Massa Corporal (IMC), dores osteomioarticulares e n?vel de sedentarismo. Pouco se sabe sobre a influ?ncia do perfil da adiposidade corporal, do g?nero e da resposta aguda do sistema cardiovascular sobre a capacidade de caminhada do obeso. Objetivo: Avaliar o efeito de medidas antropom?tricas (IMC e WHR, waist-to-hip ratio), esfor?o card?aco e sedentarismo sobre a capacidade de caminhada de portadores de obesidade m?rbida. Materiais e M?todos: Entre setembro de 2007 e setembro de 2008, 36 obesos m?rbidos (idade 36,23 + 11,82; IMC 49,16 kg/m2) foram recrutados no ambulat?rio de tratamento da obesidade e cirurgia bari?trica do Hospital Universit?rio Onofre Lopes e avaliados quanto marcadores antropom?tricos de obesidade, fun??o pulmonar, n?vel de atividade f?sica habitual (Question?rio de Baecke) e capacidade de caminhar (TC6M). O paciente era monitorado para verificar: freq??ncia card?aca (FC) e respirat?ria (FR), satura??o perif?rica de oxig?nio, n?vel de esfor?o percebido, press?o arterial sist?mica (PA) e duplo-produto (DP). Durante a caminhada, ainda mensurava-se a velocidade m?dia desenvolvida e dist?ncia total percorrida pelos portadores de obesidade m?rbida. Os dados foram analisados entre os g?neros e o tipo de distribui??o de gordura corporal, avaliando o comportamento das vari?veis a cada minuto caminhado. As correla??es de Pearson e Spearmam foram analisadas. A Regress?o M?ltipla buscou preditores da capacidade de caminhada. Foi utilizado o software Statistic 6.0 para an?lise estat?stica. Resultados: 20 obesos tinham adiposidade abdominal (WHR = 1,01), circunfer?ncia da cintura de 135,8 cm nas mulheres (25) e de 139,8 cm nos homens (10). Durante TC6M, foi caminhada uma dist?ncia de 412,43 m, sem diferen?as entre g?nero ou adiposidade. Essa dist?ncia total percorrida foi explicada isoladamente pelo IMC (45%), FC no sexto minuto (43%), Baecke (24%) e fadiga (-23%). 88,6% dos obesos (31) realizaram o teste acima de 60% da FCM?xima, sendo o pico de FC atingido aos 5 minutos de caminhada. PA e DP aumentaram significativamente com a caminhada, mas sem diferen?as entre g?nero ou adiposidade. Conclus?o: A acaminhada dos obesos m?rbidos n?o sofre influ?ncia do g?nero ou do perfil de adiposidade corporal. A dist?ncia final percorrida ? atribu?da ao excesso de peso corporal, estress card?aco, sensa??o de esfor?o imposta pela caminhada e ao n?vel de sedentarismo pr?vio do obeso. Dentro de 1 minuto de caminhada, os obesos atingem uma zona de treinamento cardiovascular