Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-07-04T19:38:22Z No. of bitstreams: 1 TES_CRISTIANE_RIOS_PETRARCA_COMPLETO.pdf: 2589282 bytes, checksum: 52270debca013b954c56d75d899d3ff8 (MD5) / Made available in DSpace on 2016-07-04T19:38:22Z (GMT). No. of bitstreams: 1 TES_CRISTIANE_RIOS_PETRARCA_COMPLETO.pdf: 2589282 bytes, checksum: 52270debca013b954c56d75d899d3ff8 (MD5) Previous issue date: 2016-03-18 / Introduction: Colorectal cancer is the third most common cancer in men and the second in women worldwide. It presents multifactorial, heterogeneous and complex etiopathogeny, still not fully elucidated. The evolution of the disease is often distinct from the exhaustive surgical and pathological staging, eventually (possibly) patients with focal disease develop an aggressive pattern presenting poor prognosis. Recent evidence shows the molecular heterogeneity of colorectal cancer. In this context the microRNAs (miRNAs), small non-coding RNAs (containing 19-25 nucleotides) capable of regulating gene expression in post-transcriptional level, have been identified with different expressions for numerous diseases, including cancer. Colorectal cancer shows change in expression of several miRNAs. These changes have been associated with the diagnosis, prognosis, gene expression, chemosensitivity and staging, being a potential biomarker. Methods: Clinical and pathological data of patients submited to surgical resection of the primary tumor and regional lymph nodes diagnosed from September 2002 to October 2011 have been reviewed and analyzed, and included in the colorectal cancer tumor bank of the S?o Lucas Hospital's Oncology Department - PUCRS. The analysis of the expression of miRNAs was performed in the laboratory of the Institute of Biotechnology of the Catholic University of Brasilia where they were analyzed in primary tumor and regional lymph nodes for descriptive purposes and versus the clinical-pathological data of the cases studied. The quantification analysis of the following miRNA (mir-570, mir-16, mir-338, Let-7, miR-1, miR-150, mir-183, mir-650 and mir-31) were determined by qPCR. Statistical analysis tests: Fisher's, Wilcoxon and Kruskal Wallis Exact, considered a significance level of 5%. Results: Of the 28 cases studied, 28.6% were less than 60 years old at diagnosis and 71.4% aged 60 or over. The average age was 66.7 years (26 to 86). The mean (average) follow-up (period, age) was 3.9 years (0 to 9), SD= 2.8 and a median of 4 years. The expression of miRNAs in the primary tumor (N=28) showed more homogeneous pattern, with a tendency to overexpression; whereas, in ill lymph nodes (N=15) this pattern was more heterogeneous, with mir-570, mir-338, mir-1, mir-183 and mir-31 being presented overexpressed and mir-16, Let-7, mir-150 and mir-650 with a more repressed expression. The suppressed expression of mir-570 was associated with mortality when evaluated in the primary tumor (N=28), where the prevalence of death in individuals with suppressed expression was 63.64% and those overexpressed was 17.65%, with p=0.020. In the primary tumor of patients with lymph node metastasis (N=15) the median expression of mir-183 was 4.42 with interquartile range (IQR) p25 of 1,66 and p75 of 43.01; whereas in the samples from patients with no ill lymph nodes (N=13) the median expression of mir-183 was 54.17 with IQR p25 of 13.12 and p75 of 223.14, with p=0.01, suggesting focal disease at diagnosis. In the subgroup of patients with lymph node metastasis (N=15), the expression of mir-650 in lymph node was associated with the prevalence of recurrence. The expression of mir-650 showed suppressed expression in 25% of the recurrence cases and was overexpressed in 85% of cases, with p=0.04. Conclusions: The expression pattern of miRNAs differs depending on the site of the disease studied (primary tumor or metastatic disease). The suppressed expression of mir-570 in the primary tumor is likely to be predictor of mortality. Overexpression of mir-183 in the primary tumor suggests focal disease at diagnosis. The overexpression of mir-650 in the metastatic lymph node is a recurrence predictor. New studies including functional tests and meta-analyzes may confirm these findings and optimize the use of these miRNAs in clinical practice. / Introdu??o: O c?ncer colorretal ? o terceiro c?ncer mais comum em homens e o segundo em mulheres no mundo. Apresenta etiopatogenia multifatorial, heterog?nea e complexa, ainda n?o totalmente elucidada. A evolu??o da doen?a ? muitas vezes distinta do exaustivo estadiamento cir?rgico e patol?gico, eventualmente pacientes com doen?a localizada evoluem com padr?o agressivo apresentando mal progn?stico. Evid?ncias recentes demonstram a heterogeneidade molecular do c?ncer colorretal. Neste contexto os microRNAs (miRNAs), pequenos RNAs com 19-25 nucleot?deos n?o codificadores, que s?o capazes de regular a express?o de genes em n?vel p?s-transcricional, t?m sido identificados com diferentes express?es em diversas doen?as, inclusive no c?ncer. O c?ncer colorretal apresenta altera??o na express?o de diversos miRNAs. Estas altera??es t?m sido associadas ao diagn?stico, progn?stico, express?o de genes, quimiossensibilidade e estadiamento, sendo potencial biomarcador. M?todos: Foram revisados e analisados dados cl?nicos e anatomopatol?gicos de pacientes submetidos a ressec??o cir?rgica do tumor prim?rio e linfonodos regionais diagnosticados de setembro de 2002 ? outubro de 2011 e inclu?dos no banco de tumores de c?ncer colorretal do Servi?o de Oncologia do Hospital S?o Lucas ? PUCRS. A an?lise da express?o dos miRNAs foi realizada no laborat?rio do Instituto de Biotecnologia da Universidade Cat?lica de Bras?lia onde foram analisadas nos tumores prim?rios e nos linfonodos regionais, com fins descritivos e frente aos dados cl?nico-patol?gicos dos casos estudados. A an?lise da quantifica??o dos seguintes miRNA (mir-570, mir-16, mir-338, Let-7, mir-1, mir-150, mir-183, mir-650 e mir-31) foi realizada por qPCR. Para an?lise estat?stica os testes: Exato de Fisher, Wilcoxon e de Kruskal Wallis, considerado n?vel de signific?ncia de 5%. Resultados: Dos 28 casos estudados, 28,6% tinham menos de 60 anos na ocasi?o do diagn?stico e 71,4% 60 anos ou mais. A idade m?dia foi de 66,7 anos (26 ? 86). A m?dia de seguimento foi de 3,9 anos (0 ? 9), DP=2,8 e mediana de 4 anos. A express?o dos miRNAs no tumor prim?rio (N=28) apresentou padr?o mais homog?neo, com uma tend?ncia a superexpress?o; enquanto que, nos linfonodos doentes (N=15) este padr?o foi mais heterog?neo, com o mir-570, mir-338, mir-1, mir-183 e mir-31 apresentando-se superexpressos e mir-16, Let-7, mir-150 e mir-650 com express?o reprimida. A express?o reprimida do mir-570 apresentou associa??o com mortalidade quando avaliado no tumor prim?rio (N=28), onde a preval?ncia de ?bito nos indiv?duos com express?o reprimida foi de 63,64% e naqueles superexpressos foi de 17,65%, com valor p=0,020. No tumor prim?rio dos pacientes com met?stase linfonodal (N=15) a mediana de express?o do mir-183 foi de 4,42 com intervalo interquartil p25 de 1,66 e p75 de 43,01; enquanto que, nas amostras de pacientes com linfonodos n?o doentes (N=13) a mediana de express?o do mir-183 foi de 54,17 com intervalo interquartil p25 de 13,12 e p75 de 223,14, com valor p= 0,01, sugerindo doen?a localizada ao diagn?stico. No subgrupo de pacientes com met?stase linfonodal (N=15), a express?o do mir-650 no linfonodo foi associada a preval?ncia de recidiva. A express?o do mir-650 esteve reprimida em 25% dos casos que recidivaram e superexpresso em 85% dos casos, com valor p=0,04. Conclus?es: O padr?o de express?o dos miRNAs difere conforme o s?tio da doen?a estudada (tumor prim?rio ou doen?a metast?tica). A express?o reprimida do mir-570 no tumor prim?rio provavelmente seja preditora de mortalidade. Superexpress?o do mir-183 no tumor prim?rio sugere doen?a localizada ao diagn?stico. A superexpress?o do mir-650 no linfonodo metast?tico ? preditor de recidiva. Novos estudos incluindo testes funcionais e metan?lises poder?o ratificar estes achados e otimizar a utiliza??o destes miRNAs na pr?tica cl?nica.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-07-12T18:58:42Z No. of bitstreams: 1 DIS_MORGANA_THAIS_CAROLLO_FERNANDES_PARCIAL.pdf: 412256 bytes, checksum: ed9b15670049bf6fd6c6f737d72389a7 (MD5) / Made available in DSpace on 2016-07-12T18:58:42Z (GMT). No. of bitstreams: 1 DIS_MORGANA_THAIS_CAROLLO_FERNANDES_PARCIAL.pdf: 412256 bytes, checksum: ed9b15670049bf6fd6c6f737d72389a7 (MD5) Previous issue date: 2016-03-31 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Objectives: The objective of this study is to analyze the effects of asthma and body mass on lung function in children. Methodology: Asthmatic children and healthy controls were recruited in public and private schools in Porto Alegre, Brazil. Spirometry, before and after bronchodilator was obtained in the Lung Function Laboratory (PUCRS) at the same time weight and height were measured. Lung function and body mass index were transformed to z-scoresfor all analysis using international equations. Results: 188 children (97 girls, 52%) were enrolled, 114(60%) with asthma and 85(45%) overweight. The mean and standard deviation of age, height and weight were 11.1+1.1, 146.7+8.4 and 44.2+12.3 respectively. There was a positive correlation between BMI and FVC and FEV1 and a negative correlation with FEV1/FVC. Children with asthmahad lower FEV1/FVC and FEF25-75 and higher FVC (p<0.05 for all). Overweight children had higher FVC and FEV1 and lower FEV1/FVC (p<0.05 for all). Stratifying the analysis by asthma (yes/no) and BMI (normal weight/overweight) we found significant differences between overweight asthmatic children and overweight/non-overweight controls for FEV1/FVC and FEF25-75 (ANOVA, p<0.01 for all comparisons). Overweight asthmatic children also had lower FEV1/FVC when compared to non-overweight asthmatic children (p<0.01 for all). There was a significant trend for higher FVC and lower FEV1/FVC and FEF25-75 in overweight children with asthma. (Jonckheere-Terpstra, p<0.01 for all analysis). Conclusion: It is noticed significant effect independent of asthma and overweight in lung function in schoolchildren. Both asthma and overweight reduce the FEV1 / FVC ratio / Objetivo: analisar o efeito de sobrepeso e da asma na fun??o pulmonar, avaliada por espirometria, em crian?as. M?todos: Crian?as asm?ticas e controles saud?veis foram recrutados em escolas p?blicas de Porto Alegre, Brasil. A espirometria foi obtida da fun??o pulmonar no Laborat?rio da PUCRS concomitante com a mensura??o do peso e altura. O ?ndice da fun??o pulmonar e a massa corporal foram transformados em z ? scores para todas as an?lises usando equa??es internacionais. Resultados: 188 crian?as (97 meninas, 52%) estavam matriculadas sendo 114 (60%) com asma e 85 (45%) com sobrepeso. A m?dia e o desvio padr?o da idade, altura e peso foram de 11,1 +/- 1,1; 146,7 +/- 8,4 e 44,2 +/- 12,3, respectivamente. Houve uma correla??o positiva entre IMC e CVF e FEV1 e uma correla??o negativa com FEV1/CVF. Crian?as com asma apresentaram menor FEV1/CVF e FEF25-75 e maior CVF (p<0,05). Crian?as com sobrepeso apresentaram maior CVF e FEV1 e menor FEV1/CVF (p < 0,05). Ao estratificar a an?lise por asma (sim/n?o) e IMC (peso normal/sobrepeso) encontramos diferen?as significativas entre as crian?as asm?ticas com sobrepeso e controles com sobrepeso para FEV1 /FVC e FEF25-75 (ANOVA, p <0,01 para todas as compara??es). Crian?as asm?ticas com sobrepeso tamb?m tiveram menor FEV1/CVF quando comparadas com crian?as asm?ticas eutr?ficas (p < 0,01). Houve uma tend?ncia significativa para uma maior CVF e inferior FEV1/ CVF e FEF25-75 em crian?as com sobrepeso asm?ticas (Jonckheere - Terpstra, P < 0,01 para todas as an?lises). Conclus?es: Percebe-se efeito significativo e independente de asma e sobrepeso na fun??o pulmonar de escolares. Tanto asma como sobrepeso reduzem a ?rela??o VEF1/CVF?.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-07-19T18:34:17Z No. of bitstreams: 1 DIS_BRUNO_LUIZ_GUIDOLIN_PARCIAL.pdf: 2466438 bytes, checksum: 72b981ee9f776fee3d19f527647d58be (MD5) / Made available in DSpace on 2016-07-19T18:34:18Z (GMT). No. of bitstreams: 1 DIS_BRUNO_LUIZ_GUIDOLIN_PARCIAL.pdf: 2466438 bytes, checksum: 72b981ee9f776fee3d19f527647d58be (MD5) Previous issue date: 2016-03-28 / Objective:To investigate the association between current alcohol dependence and alcohol dependence during life with cognitive and mental disorders in the elderly of the city of Porto Alegre, Rio Grande do Sul, Brazil. Methods:This was a crosssectional study with application of Mini International Neuropsychiatric (M.I.N.I. Plus Brazilian version 5.0.0), Global Assessment Questionnaire for the Elderly (QAGI) and Neuropsychological do CERAD (Consortium to Establish a Registry for Alzheimer's Disease) in 580 elderly in Porto Alegre. Results: Of the 580 elderly, 24 were current dependent on alcohol (4.2%) and 85 had dependence during life (14.6%). There was a statistically significant difference (p?0,005) for sex, addiction to illegal substances, antisocial personality disorder and smoking for current alcohol dependence and alcohol dependence during life. The association between manic/hypomanic current episode and current alcohol dependence and the association between generalized anxiety disorder and alcohol dependence during life also showed statistically significant difference. Men were majority for alcohol dependence; males have 9,35 times the prevalence of current alcohol dependence and 12,81 times the prevalence of alcohol dependence during life when compared with women. Elderly diagnosed with addiction to other substances, manic/hypomanic episode, generalized anxiety disorder and smoking were more likely to have alcohol dependence compared to those who did not have such disorders. Conclusion: Cognitive disorders were not associated with alcohol dependence. Some psychiatric disorders were associated with current alcohol dependence and alcohol dependence during life, which warns about the importance of early diagnosis of these disorders in the elderly. / Objetivo: Investigar a associa??o entre a depend?ncia de ?lcool atual e a depend?ncia de ?lcool na vida com os transtornos cognitivos e mentais em pacientes idosos do Munic?pio de Porto Alegre, Rio Grande do Sul, Brasil. M?todos: Trata-se de um estudo transversal com aplica??o do Mini International Neuropsychiatric interview, M.I.N.I. Plus Brazilian version 5.0.0, do Question?rio de Avalia??o Global do Idoso (QAGI) e da Avalia??o Neuropsicol?gica do CERAD (Consortium to Establish a Registry for Alzheimer Disease) em 580 idosos de Porto Alegre. Resultados: Dos 580 idosos, 24 eram dependentes de ?lcool (4,2%) e 85 apresentaram depend?ncia durante a vida (14,6%). Houve diferen?a estatisticamente significativa (p?0,005) para sexo, depend?ncia de subst?ncias il?citas, transtorno de personalidade antissocial e tabagismo para a depend?ncia de ?lcool atual e depend?ncia de ?lcool na vida. Quando comparado epis?dio man?aco/hipoman?aco atual com a depend?ncia de ?lcool atual, e transtorno de ansiedade generalizada com depend?ncia de ?lcool na vida, houve diferen?a estatisticamente significativa. Os homens foram maioria na depend?ncia de ?lcool; o sexo masculino teve 9,35 vezes a preval?ncia de depend?ncia de ?lcool atual e 12,81 vezes a preval?ncia de depend?ncia de ?lcool na vida quando comparado com o sexo feminino. Os idosos diagnosticados com depend?ncia de outras subst?ncias, epis?dios man?aco/hipoman?acos, transtorno de ansiedade generalizada e tabagismo foram mais prop?cios a ter depend?ncia de ?lcool quando comparados ?queles que n?o apresentaram tais transtornos. Conclus?o: Os transtornos cognitivos n?o foram associados com a depend?ncia de ?lcool. Alguns transtornos psiqui?tricos, entretanto, apresentaram associa??o com a depend?ncia de ?lcool atual e na vida, o que alerta para a import?ncia de um diagn?stico ao longo da vida desses transtornos nos idosos.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-07-19T18:40:04Z No. of bitstreams: 1 DIS_MARIA_LUIZA_SILVEIRA_BRAGHIROLLI_COMPLETO.pdf: 2020231 bytes, checksum: 184b2ea8bc0c499060a07147636467fe (MD5) / Made available in DSpace on 2016-07-19T18:40:04Z (GMT). No. of bitstreams: 1 DIS_MARIA_LUIZA_SILVEIRA_BRAGHIROLLI_COMPLETO.pdf: 2020231 bytes, checksum: 184b2ea8bc0c499060a07147636467fe (MD5) Previous issue date: 2016-03-21 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The aim of this study is to analyse the executive functioning of the frontal lobe in obese teenagers Veran?polis (RS), to prevention and health promotion in active aging. It?s a cross-sectional, descriptive analytic observational with the collection data and retrograde analysis. Schoolers between 16 and 18 years of public and private high school of Veran?polis (RS) were evaluated. The study group consisted of 20 teenagers obese and overweight, and body mass index (BMI) at or above the percentile 97. The control group consisted of 30 non-obese teenagers (eutrophics) with BMIs between percentiles three and 85 of the same population and were equivalent the first group by age and sex. Anthropometric data and demographic data were obtained of the database from the original project. The neuropsychological assessment data were obtained in the period in which adolescents underwent MRI in Inscer. The Frontal Systems Behavior Scale (FrSBe Scale) and the Beck Depression Scale (BDI) were used. Descriptive statistics were used in the demographic data of the sample and in the evaluation indicators. The t Test was used to compare the averages of the indicators between the groups and in the effect size. The option for independent samples was chosen because individuals were different between the study group and the control group, even though they were from the same veronense population. The Pearson correlation test found the relationship between indicators. The performance of executive functioning of obese teenagers differs from non-obese young people, because they have apathy, characterized by impairments in behavior which contributing to obesity. Anthropometric indicators BMI and abdominal circumference (AC) have a positive correlation with apathy. The evaluation of depressive symptoms didn?t showed significant difference between obese and non-obese teenagers. Obese adolescents tend to show higher levels of depressive symptoms than subjects with normal weight, considering the average points presented by those groups. Anthropometric indicators BMI and AC don?t correlate with depression symptoms and don?t influence the mood of obese young people. / O objetivo desse estudo ? avaliar o funcionamento executivo do lobo frontal em jovens obesos de Veran?polis (RS), visando preven??o e promo??o de sa?de no envelhecimento ativo. ? um estudo transversal, descritivo anal?tico, observacional com coleta de dados e an?lise retr?grada. Foram avaliados 50 estudantes entre 16 e 18 anos, da rede p?blica e privada do ensino m?dio de Veran?polis. O grupo de estudo foi composto por 20 indiv?duos obesos e com sobrepeso, e ?ndice de massa corporal (IMC) igual ou superior ao percentil 97. O grupo controle foi constitu?do por 30 escolares n?o obesos (eutr?ficos), com IMCs entre os percentis tr?s e 85 da mesma popula??o, equiparados ao primeiro grupo por sexo e idade. Os dados antropom?tricos e os dados demogr?ficos foram obtidos do banco de dados do projeto original. A coleta de dados da avalia??o neuropsicol?gica foi obtida no per?odo em que os adolescentes realizaram exame de RM no Inscer. A testagem utilizada consistiu da Escala FrSBe (Frontal Systems Behavior Scale) e da Escala Beck da Depress?o (BDI). A estat?stica descritiva foi empregada na caracteriza??o demogr?fica da amostra e avalia??o dos indicadores. O Teste T foi utilizado na compara??o das m?dias dos indicadores entre os grupos e o tamanho de efeito, avaliado. A op??o para amostras independentes foi escolhida, pois os indiv?duos eram distintos entre o grupo que tem obesidade e o grupo controle, mesmo sendo oriundos da mesma popula??o de adolescentes veronenses. O Teste de Correla??o de Pearson verificou a rela??o entre os indicadores. O desempenho do funcionamento executivo dos jovens obesos diferencia-se dos jovens n?o obesos, por apresentarem apatia, caracterizada por preju?zos no comportamento, contribuindo para obesidade. Os indicadores antropom?tricos IMC e CA apresentam correla??o positiva com a apatia. A avalia??o dos sintomas depressivos n?o mostrou diferen?a significativa entre jovens obesos e n?o obesos, sendo que os jovens obesos tendem a apresentar n?vel maior de sintomas depressivos em rela??o aos sujeitos com peso normal, considerando a m?dia de pontos apresentada pelos grupos. Os indicadores antropom?tricos n?o apresentam correla??o com sintomas da depress?o, isto ?, o IMC e a CA n?o influenciam o humor dos jovens obesos.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-07-21T18:39:03Z No. of bitstreams: 1 DIS_MARIA_MERCEDES_CARACCIOLO_PICARELLI_COMPLETO.pdf: 1479254 bytes, checksum: 1fc931d06c940f809dbcf8825a640251 (MD5) / Made available in DSpace on 2016-07-21T18:39:03Z (GMT). No. of bitstreams: 1 DIS_MARIA_MERCEDES_CARACCIOLO_PICARELLI_COMPLETO.pdf: 1479254 bytes, checksum: 1fc931d06c940f809dbcf8825a640251 (MD5) Previous issue date: 2016-03-31 / Introduction: Recent advances in juvenile idiopathic arthritis (AIJ) treatment promoted free disease survival. Cardiovascular disease (DCV) may emerge as an important cause of morbidity and mortality. Pulse wave velocity (VOP) and telomere length (TL) are considered as potential predictors of cardiovascular DCV. There are growing evidences of chronic and persistent inflammatory activity role on these processes. VOP and TL are potential early predictors of DCV and its outcomes. Objective: Access VOP and TL in a sample of AIJ patients, without cardiovascular risk factors, compare them with a group of healthy individuals sex and age matched and to test the correlation of these variables. Patients and Methods: 24 AIJ patients and 21 controls for TL and 20 controls for VOP were included. VOP was estimated by an oscillometric device (CardioSDyna ? MAPA +). TL was accessed by polimerase chain reaction in real time (qPCR). Inflammatory activity was accessed by Juvenile Disease Activity Score (JADAS-27).Obesity, systemic hypertension, diabetes mellitus, impaired renal function and other inflammatory diseases were excluded. Results: Oligoarticular (65, 2%) and polyarticular subtypes were included, with 73,9% females and 82,6% were Caucasian. The average age was 15,5 + 6,3 years and the median disease duration was 9 [5-19] years. Between cases and controls for LTL there was significant difference in age no differences in sex, ethnics and BMI. The JADAS median was 8 [0, 00 ? 20, 1], considered active when above 1, 0. PWV was normal in all patients, JIA and control (5.1 + 0.20 m/s vs. 4.98 + 0.06 m/s, P = 0, 66). TL expressed by T/S ratio (amplification telomere product and single copy gene) was significantly reduced between JIA patients and controls (0. 85 + 0, 34 vs. 1, 67 + 1, 38, Mann-Whitney test P = 0.025). When age adjusted by ANCOVA, the difference remained significant (P= 0,032). There was no correlation between TL and age (P =0, 449, r=0, 166), sex (P=0, 521), disease duration (P =0, 358, r=-0, 318), JADAS (P = 0, 184, r=-0, 287) e VOP (P = 0, 843, r=0, 044) in patients with AIJ. Conclusions: TL was significantly shorter in long disease duration and high to moderate disease activity with no DCV risk factors AIJ patients and compared to controls.VOP was normal and no significant difference between controls were found. No correlations were observed between TL or VOP and disease duration, age and sex. / Introdu??o: os avan?os na terap?utica da artrite idiop?tica juvenil (AIJ) promoveram o prolongamento da sobrevida livre de doen?a. A doen?a cardiovascular (DCV) pode emergir como importante causa de morbimortalidade. A velocidade de onda de pulso (VOP) e o comprimento de tel?meros (TL) s?o vistos como potenciais preditores de DCV. H? evid?ncias crescentes da import?ncia do papel da atividade inflamat?ria cr?nica e persistente nesses processos. VOP e TL s?o potenciais preditores precoces de DCV e seus desfechos. Objetivo: Avaliar a VOP e TL em uma amostra de pacientes com AIJ, sem fatores de risco para DCV, compar?-los com um grupo de indiv?duos saud?veis pareados por sexo e idade e testar a correla??o destas vari?veis. Pacientes e M?todos: 24 pacientes com AIJ e 21 controles para TL e 20 controles para VOP foram inclu?dos. A VOP foi estimada por um equipamento de an?lise oscilom?trica (CardioSDyna ? MAPA +). O TL foi avaliado atrav?s da rea??o em cadeia da polimerase em tempo real (qPCR). A atividade inflamat?ria foi medida atrav?s do Juvenile Disease Activity Score (JADAS-27). Pacientes com obesidade, hipertens?o arterial sist?mica, diabetes mellitus e perda de fun??o renal e outras doen?as inflamat?rias foram exclu?dos. Resultados: Foram inclu?dos os subtipos oligoarticular (65, 2%) e poliarticular, sendo que 73,9% eram meninas, 82,6% eram caucasianos. A m?dia de idade foi 15,5 + 6, 3 anos e a mediana de dura??o da doen?a foi de 9 [5-19] anos. Entre casos e controle para TL, houve diferen?a significativa em rela??o ? idade e n?o foi encontrada diferen?a quanto a sexo, etnia, IMC, peso e altura. A mediana do JADAS foi 8 [0,00 ? 20,1],considerado ativo acima de 1 . Os valores da VOP foram normais em todos os pacientes (5,1 + 0,2 vs.4,98 + 0,06 m/s) (P = 0,66). TL expresso pela raz?o T/S (produto da amplifica??o dos tel?meros e gene de c?pia ?nica) estava reduzido nos pacientes com AIJ (0, 85 + 0, 34 vs.1,67 ? 1,38), Teste de Mann-Whitney: P=0, 025. Quando ajustado para idade atrav?s da ANCOVA, a diferen?a permaneceu significativa (P=0, 032). N?o houve correla??o entre TL e idade (P =0, 449, r=0, 166), sexo (P=0, 521) , dura??o da doen?a (P =0, 358, r=-0, 318), JADAS (P = 0, 184, r=-0, 287) e VOP (P = 0, 843, r=0, 044) em crian?as com AIJ. Conclus?o: O TL foi significativamente menor nessa amostra de pacientes com AIJ de longa dura??o e com atividade da doen?a alta a moderada e sem fatores de risco para doen?a cardiovascular em compara??o aos controles. A VOP foi normal e sem diferen?a significativa em rela??o aos controles. N?o foram observadas correla??es significativas do TL ou do VOP com atividade e dura??o da doen?a, idade e sexo.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-07-26T14:11:19Z No. of bitstreams: 1 TES_RAQUEL_DA_LUZ_DIAS_COMPLETO.pdf: 2184806 bytes, checksum: 196521807ad1dcdfa8bffc204202abe6 (MD5) / Made available in DSpace on 2016-07-26T14:11:19Z (GMT). No. of bitstreams: 1 TES_RAQUEL_DA_LUZ_DIAS_COMPLETO.pdf: 2184806 bytes, checksum: 196521807ad1dcdfa8bffc204202abe6 (MD5) Previous issue date: 2016-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Introduction: leucine (Leu) is an essential branched-chain amino acid, present in dairy products, which has been investigated for exert an important role in cell signaling. However, most studies evaluating cellular responses mediated by Leu, works within a normal perspective in AA supply and little is known about the effects that supplementation can generate on the cell proliferation mechanisms. The effects of excess of this amino acid, have been extensively studied in many cell types, but there is an important limitation on the amount of information available in the scientific literature regarding their actions in bone cells. Objective: the aim of this study to determine the effects of leucine supplementation on proliferation of pre-osteoblasts of the MC3T3-E1 lineage. Methods: the MC3T3-E1 cells were kept in ?-MEM supplemented with 10% fetal bovine serum and 1% antibiotic. After initial determination of concentrations, the cells were treated during 48 hours, by the addition of 50 ?M Leu, which corresponds to 12,5% in addition of the amino acid to the culture medium. Untreated cells represented the control group. The evaluation of the viability and proliferation of cultured cells was performed with Trypan Blue dye (0.4%). To identify the mechanisms related to decreased cellular proliferation, assays were performed to verify cytotoxicity (LDH); apotosis (Annexin V); oxidative stress (TBARS and DCFH); inflammation (TGF-? 1 and CBA); autophagy (acridine orange and flow cytometry); senescence (DAPI and flow cytometry); and DNA damage (alkaline comet assay). Results and conclusions: Leu supplementation (50 ?M) decreases cell proliferation by 40% with causes not related to cell necrosis, apoptosis, oxidative stress, inflammation or autophagy. The Leu supplementation caused DNA damage, with consequent increase in senescence and decrease of proliferation of MC3T3-E1 cells. / Introdu??o: a leucina (Leu) ? um amino?cido (AA) essencial, de cadeia ramificada, presente na alimenta??o humana, principalmente no leite e seus derivados, que tem sido investigado por exercer um importante papel na sinaliza??o celular. Contudo, a maioria dos estudos que avalia as respostas celulares mediadas pela Leu, trabalha dentro de uma perspectiva de normalidade na oferta do AA e pouco se sabe sobre os efeitos que a suplementa??o pode gerar sobre os mecanismos de prolifera??o celular. Os efeitos do excesso deste amino?cido, t?m sido extensivamente estudado em diversos tipos de c?lulas, entretanto existe uma limita??o importante na quantidade de informa??es dispon?veis na literatura cient?fica em rela??o as suas a??es em c?lulas do tecido ?sseo. Objetivo: este estudo teve como objetivo analisar os efeitos da suplementa??o de leucina sobre a prolifera??o de pr?-osteoblastos da linhagem MC3T3-E1. M?todos: a cultura dos pr?-osteoblastos da linhagem MC3T3-E1, foi realizada com ?-MEM, suplementado com 10% de soro fetal bovino e 1% de antibi?tico. Ap?s determina??o de concentra??es, o tratamento foi feito com a adi??o de Leu, dilu?da ao meio de cultura nas concentra??es de 50 ?M, o que corresponde a um acr?scimo percentual de 12.5% a mais do amino?cido ao meio de cultura, por 48 horas. A viabilidade e a prolifera??o celular foram avaliadas pela t?cnica do Trypan Blue. Para a identifica??o dos mecanismos relacionados a inibi??o da prolifera??o celular, foram realizados ensaios que avaliaram a citotoxicidade (LDH); apoptose (Anexina V); estresse oxidativo (TBARS e DCFH); perfil inflamat?rio (TGF-? 1 e CBA); autofagia (laranja de acridina e citometria de fluxo); senesc?ncia (DAPI) e dano ao DNA (teste cometa). Resultados e conclus?es: a suplementa??o de Leu (50 ?M) inibe a prolifera??o celular em 40%, com causas n?o relacionadas a necrose celular, apoptose, estresse oxidativo, inflama??o ou autofagia. A suplementa??o de Leu provocou dano ao DNA, com consequente senesc?ncia e diminui??o da prolifera??o celular de pr?-osteoblastos da linhagem MC3T3-E1.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-08-04T16:43:24Z No. of bitstreams: 1 DIS_FRANCISCO_JOSE_PASCOAL_RIBEIRO_JUNIOR_COMPLETO.pdf: 7999023 bytes, checksum: 93fe69c981c9fb86c268dc124a4441cd (MD5) / Made available in DSpace on 2016-08-04T16:43:24Z (GMT). No. of bitstreams: 1 DIS_FRANCISCO_JOSE_PASCOAL_RIBEIRO_JUNIOR_COMPLETO.pdf: 7999023 bytes, checksum: 93fe69c981c9fb86c268dc124a4441cd (MD5) Previous issue date: 2016-03-01 / The aim of this study was to determine the association between smoking and cognitive and mental disorders in the elderly and their association with sociodemographic characteristics in a sample of patients in the city of Porto Alegre, Rio Grande do Sul, Brazil. This was a descriptive, cross-sectional and prospective data collection study, utilizing the Mini International Neuropsychiatric interview, Global Assessment Questionnaire Aging and Neuropsychological Assessment CERAD (Consotum to Establish a Registry for Alzheimer's Disease) in 580 elderly aged 60 or more. 580 elderly, there is a higher prevalence for men (73.3%), aged between 60-69 years (24.0%), single (65.3%), widowed (61.3%) without income (28 , 9%), specific phobia (28.4%), grouped phobias (26.9%) current alcohol dependence (62.5%) and alcohol dependence in life (41.2%). There was more difficulty in quitting smoking in elderly: major depressive episode throughout life (67.0%), specific phobia (65.4%), grouped phobias (66.4%), current alcohol dependence (64, 7%) and lifelong (65.5%) and any anxiety disorder (66.7%). Seniors with current alcohol dependence and grouped phobias have 2.8 and 1.5 times the prevalence of current smoking, respectively. Older people at risk of suicide have 1.3 times higher the prevalence of smoking in the present life. And older adults with alcohol dependence in life and bipolar disorder have 1.4 and 1.6 times higher smoking prevalence in life, respectively. Elderly people with dementia have 1.4 times greater prevalence to have stopped smoking compared to older people without dementia. These results indicate the presence of mental disorders in elderly smokers, calling attention to some sociodemographic characteristics and associations with some disorders that can make a difference in the diagnosis and treatment of these disorders. / O objetivo desse estudo ? determinar a associa??o entre tabagismo e os transtornos cognitivos e mentais em idosos e suas associa??es com as caracter?sticas s?cio-demogr?ficas em uma amostra de pacientes do munic?pio de Porto Alegre, Rio Grande do Sul, Brasil. Este foi um estudo descritivo, transversal e com coleta prospectiva, com aplica??o do Mini International Neuropsychiatric interview, Question?rio de Avalia??o Global do Idoso e Avalia??o Neuropsicol?gica do CERAD (Consotum to Establish a Registry for Alzheimer Disease) em 580 idosos com 60 anos ou mais. Dos 580 idosos, h? maior preval?ncia para homens (73,3%), faixa et?ria entre 60-69 anos (24,0%), solteiros (65,3%), vi?vos (61,3%), sem renda (28,9%), fobia espec?fica (28,4%), fobias agrupadas (26,9%), depend?ncia de ?lcool atual (62,5%) e depend?ncia de ?lcool na vida (41,2%). Houve maior dificuldade em parar de fumar em idosos com: epis?dio depressivo maior ao longo da vida (67,0%), fobia espec?fica (65,4%), fobias agrupadas (66,4%), depend?ncia de ?lcool atual (64,7%) e ao longo da vida (65,5%) e qualquer transtorno de ansiedade (66,7%). Idosos com depend?ncia de ?lcool atual e fobias agrupadas apresentam 2,8 e 1,5 vezes maior a preval?ncia de tabagismo atual, respectivamente. Idosos com risco de suic?dio possuem 1,3 vez maior a preval?ncia de apresentarem tabagismo na vida. E idosos com depend?ncia de ?lcool na vida e transtorno do humor bipolar apresentam 1,4 e 1,6 vezes maior a preval?ncia de tabagismo na vida, respectivamente. Idosos com dem?ncia possuem 1,4 vezes maior preval?ncia para terem parado de fumar se comparados aos idosos sem dem?ncia. Esses resultados apontam para a presen?a de transtornos mentais em idosos tabagistas, chamando a aten??o para algumas caracter?sticas sociodemogr?ficas e associa??es com alguns transtornos, que podem fazer a diferen?a no diagn?stico e tratamento dessas patologias.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-08-22T16:31:50Z No. of bitstreams: 1 DIS_GUILHERME_ZIMMERMANN_PERRONE_COMPLETO.pdf: 1329077 bytes, checksum: 4469222c4fed920d365eace7a4726e65 (MD5) / Made available in DSpace on 2016-08-22T16:31:50Z (GMT). No. of bitstreams: 1 DIS_GUILHERME_ZIMMERMANN_PERRONE_COMPLETO.pdf: 1329077 bytes, checksum: 4469222c4fed920d365eace7a4726e65 (MD5) Previous issue date: 2016-04-29 / Fibromyalgia is a chronic disease of unknown etiology that can cause widespread pain, sleep disorders, headaches, problems with memory and concentration, mood disorders, irritable bowel syndrome and fatigue. Although there are several options available treatments, the optimal management for this disease remains unclear. Clinical guidelines recommend that treatment should be multidisciplinary, using both pharmacological therapies as non-pharmacological interventions, in order to reduce the various symptoms associated, however, the impact of these treatments is still widely discussed and approaches used in different services can vary. Objective: To present a systematic review of the non-pharmacological treatment options fibromyalgia available in the literature and through the analysis of medical records, describe the individual users of a specific clinic for this purpose in order to know the profile of these individuals, identifying possible options for non-pharmacological treatments recommended for fibromyalgia. Method: The study was divided into two stages. In a study, a systematic review of the literature regarding non-pharmacological treatment used for fibromyalgia was performed. complete articles such clinical trial were selected, pilot study, experimental study or case study, with some kind of non-pharmacological intervention for fibromyalgia, written in the languages English, Spanish and Portuguese, who had immediate results after the intervention, published in the last 5 years. Databases were used PubMed, Lilacs and Embase. We first carried out a search of online articles available. After two independent authors performed the selection of items from the reading of the titles, abstracts and, where applicable, the full articles. In cases of disagreement, the third author also made reading, deciding to include or exclude them. In study two, the review of medical records of patients users of the rheumatology clinic of S?o Lucas Hospital of the Pontifical Catholic University of Rio Grande do Sul was made. The study included female patients, aged less than eighteen (18) years, with an established diagnosis of fibromyalgia. The collection period included the medical records of patients seen until the month of December in the year 2015. Results: Non-pharmacological forms of treatment most commonly found are exercise programs, combined therapy, transcranial stimulation and educational programs. Regarding the review of 87 evaluated medical records were selected referring to 31 patients who met the inclusion criteria of the study. It was observed that the recommendation to perform only exercise programs was used for users 7, 9 for physical therapy, and 8 for both modes. It was not prescribed any other form of non-pharmacological intervention. Conclusion: Aerobic exercise and combined training programs appear to be more effective for the improvement of fibromyalgia symptoms, compared with flexibility exercises and resistance carried out in isolation. Combination therapies seem to have positive effects on symptoms of fibromyalgia compared to the interventions carried out in isolation. Furthermore, educational programs and transcranial stimulation also suggest produce improvements of the symptoms of fibromyalgia. / A fibromialgia ? uma doen?a cr?nica, de etiologia desconhecida, que pode causar dor generalizada, dist?rbios do sono, cefaleia, problemas de mem?ria e de concentra??o, dist?rbios do humor, s?ndrome do c?lon irrit?vel e fadiga. Embora existam diversas op??es de tratamentos dispon?veis, o manejo ?timo para essa doen?a permanece indefinido. Diretrizes cl?nicas recomendam que o tratamento deva ser multidisciplinar, utilizando-se tanto de terapias farmacol?gicas quanto de interven??es n?o farmacol?gicas, a fim de reduzir os diversos sintomas associados, por?m, o impacto desses tratamentos ainda ? bastante discutido e as condutas adotadas em diferentes servi?os podem variar. Objetivo: Apresentar uma revis?o sistem?tica sobre as op??es de tratamentos n?o farmacol?gicos da fibromialgia dispon?veis na literatura e, atrav?s da an?lise dos prontu?rios m?dicos, descrever os indiv?duos usu?rios de um ambulat?rio espec?fico para esse fim, a fim de conhecer o perfil destes sujeitos, identificando as poss?veis op??es de tratamentos n?o farmacol?gicos recomendados para a fibromialgia. M?todo: O presente estudo foi dividido em duas etapas. No estudo um, foi realizada uma revis?o sistem?tica da literatura em rela??o ao tratamento n?o farmacol?gico utilizado para fibromialgia. Foram selecionados artigos completos do tipo ensaio cl?nico, estudo piloto, estudo experimental ou estudo de caso, com algum tipo de interven??o n?o farmacol?gica para a fibromialgia, escritos nas l?nguas ingl?s, espanhol e portugu?s, que apresentavam resultados imediatos ap?s a interven??o, publicados nos ?ltimos 5 anos. Foram utilizadas as bases de dados PubMed, Lilacs e Embase. Primeiramente foi realizada a busca dos artigos dispon?veis online. Ap?s, dois autores independentes realizaram a sele??o dos artigos a partir da leitura dos seus t?tulos, resumos e, quando aplic?vel, dos artigos completos. Nos casos de diverg?ncias, o terceiro autor tamb?m fez a leitura, decidindo a inclus?o ou exclus?o dos mesmos. No estudo dois, foi feita a revis?o dos prontu?rios m?dicos dos pacientes usu?rios do ambulat?rio de reumatologia do Hospital S?o Lucas da Pontif?cia Universidade Cat?lica do Rio Grande do Sul. Foram inclu?dos no estudo pacientes do sexo feminino, idades igual ou superior a dezoito (18) anos, com diagn?stico estabelecido de fibromialgia. O per?odo de coleta incluiu os prontu?rios dos pacientes atendidos at? o m?s de Dezembro do ano de 2015. Resultados: As formas de tratamento n?o farmacol?gico mais comumente encontradas foram programas de exerc?cio f?sico, terapias combinadas, estimula??o transcraniana e programas educacionais. Em rela??o ? revis?o dos 87 prontu?rios m?dicos avaliados, foram selecionados 31 referentes aos pacientes que atenderam os crit?rios de inclus?o do estudo. Observou-se que a recomenda??o para realizar apenas programas de exerc?cio f?sico foi utilizada para 7 usu?rios, 9 para fisioterapia, e 8 para ambas as modalidades. N?o foi prescrita nenhuma outra forma de interven??o n?o farmacol?gica. Conclus?o: Programas de exerc?cios f?sicos aer?bios e de treinamento combinado parecem ser mais efetivos para a melhora dos sintomas da fibromialgia, quando comparados a exerc?cios de flexibilidade e resistidos realizados de forma isolada. Terapias combinadas parecem ter efeitos positivos nos sintomas da fibromialgia quando comparadas ?s interven??es realizadas de forma isolada. Al?m disso, programas educacionais e de estimula??o transcraniana tamb?m sugerem produzir melhoras dos sintomas da fibromialgia.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-08-23T16:17:32Z No. of bitstreams: 1 DIS_MARCELO_RECONDO_CHEFFE_COMPLETO.pdf: 3315226 bytes, checksum: 0fdee62535103c67bd1e877995e8c9f9 (MD5) / Made available in DSpace on 2016-08-23T16:17:32Z (GMT). No. of bitstreams: 1 DIS_MARCELO_RECONDO_CHEFFE_COMPLETO.pdf: 3315226 bytes, checksum: 0fdee62535103c67bd1e877995e8c9f9 (MD5) Previous issue date: 2016-05-25 / Background: Dynamic breast is a known entity characterized by visible distortion and deformity of the breast as a result of the contraction of the pectoralis major muscle after a subpectoral implant, fortunate occurring in small fraction of the patients. The purpose of this study is to present an original method for objectively measuring the breast distortion on patients submitted to dual plane augmentation. Methods: We studied 32 female patients, aged 18 ? 50 years, who underwent primary dual plane breast augmentation with at least one year of follow-up. Anthropometric landmarks in the breast region were marked as parameters in order to originate linear segments. Through the study of photographs of the breast region, taken under no contraction and under maximum contraction of the pectoralis muscle (MCP), measurements of the linear segments were made through the use of a software, and comparisons between the measured groups were evaluated. Results: We found differences in all the segments analyzed when comparing the measurements of the breasts under no contraction of the pectoralis muscle and under MPC (p<0,001). Conclusion: Our study proposes a new and original method for measuring the dynamic breast distortion after dual plane augmentation through photogrammetry, allowing us not only to quantify the deformity but also to reproduce this methodology in any patient, contributing to objective enlightenment, for surgeons as well as patients, regarding this entity, not unusual after subpectoral implant. / Introdu??o: Deformidade mam?ria din?mica ? uma entidade bem conhecida caracterizada por distor??o e deformidade mam?ria vis?vel resultante da contra??o do m?sculo peitoral maior ap?s implante subpeitoral, felizmente acometendo apenas parte dos pacientes. O objetivo de nosso estudo ? apresentar um m?todo original para mensurar de maneira objetiva a distor??o mam?ria em pacientes submetidos a aumento mam?rio em duplo plano. M?todos: Foram avaliadas 32 pacientes, com idade entre 18 e 50 anos, submetidas a implante mam?rio em duplo plano, com no m?nimo 1 ano de p?s-operat?rio. Pontos antropom?tricos na regi?o mam?ria foram demarcados com o prop?sito de originar segmentos lineares. Atrav?s do estudo fotogr?fico da regi?o mam?ria das pacientes, em repouso e sob contra??o m?xima do m?sculo peitoral, medidas dos segmentos lineares foram realizadas atrav?s do uso de software, e compara??es entre os grupos medidos (em repouso e sob contra??o do m?sculo peitoral) foram feitas. Resultados: Houve diferen?a significativa em todos os segmentos analizados quando comparadas as medidas dos segmentos lineares das mamas em repouso e sob contra??o m?xima do m?sculo peitoral (p<0,001). Conclus?o: Nosso estudo prop?e um novo e original m?todo que permite, atrav?s de fotogrametria, mensurar de maneira objetiva a deformidade din?mica mam?ria ap?s implante mam?rio em duplo plano, possibilitando a remo??o da subjetividade na avalia??o dos pacientes acometidos desta deformidade ap?s implante mam?rio subpeitoral.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-08-29T14:06:36Z No. of bitstreams: 1 DIS_JOEL_HIRTZ_DO_NASCIMENTO_NAVARRO_PARCIAL.pdf: 326191 bytes, checksum: b4a1a4a965e905f18947117bd092e4c7 (MD5) / Made available in DSpace on 2016-08-29T14:06:36Z (GMT). No. of bitstreams: 1 DIS_JOEL_HIRTZ_DO_NASCIMENTO_NAVARRO_PARCIAL.pdf: 326191 bytes, checksum: b4a1a4a965e905f18947117bd092e4c7 (MD5) Previous issue date: 2016-03-23 / The Primary Health Care (PHC) constitutes preferably as the first level of contact of an individual with the Unified Health System and the Family Health Strategy (FHS) is the means by which the reorganization is sought in Brazil. The Ministry of Health considers relevant to assessing the PHC through the presence and extent of the essential attributes and derivatives proposed by the Primary Care Assessment Tool (PCATool-Brazil). In this sense, the present study aimed to compare the health care of the oldest in Basic Health Units (BHU) with and without ESF. It characterized by being observational, cross-sectional and quantitative approach. Fifity elderly were interviewed, aged 80 or more, users of BHU with and without FHS in the city of Porto Alegre, Rio Grande do Sul. When comparing the BHU models only services without Family Health demonstrated a high degree of orientation to the PHC for the Essential Attributes Score and the General Score of PCATool-Brazil. It was found that although the FHS be the means by which the PHC reorganizes in the country, this model does not have the intended effectiveness for the health care of the elderly. It is understood that the findings of this study contribute to new strategies in the PHC work process is designed so that receive more universality the older people and their different capacities, including those longer-lived. / A Aten??o Prim?ria ? Sa?de (APS) constitui-se preferencialmente como o primeiro n?vel de contatode um indiv?duo com o Sistema ?nico de Sa?de e, a Estrat?gia de Sa?de da Fam?lia (ESF) ? o meio pelo qual busca-se a sua reorganiza??o no Brasil. O Minist?rio da Sa?de considera relevante a avalia??o da APS atrav?s da presen?a e extens?o dos atributos essenciais e derivados propostos pelo Instrumento de Avalia??o da Aten??o Prim?ria (PCATool-Brasil). Nesse sentido, o presente estudo teve como objetivo comparar a aten??o ? sa?de de longevos em Unidades B?sicas de Sa?de (UBS) com e sem ESF. Caracterizou-se por ser observacional, transversal e com abordagem quantitativa. Foram entrevistados 50 pessoas idosas com 80 anos ou mais, usu?rias de UBS com e sem ESF do munic?pio de Porto Alegre, Rio Grande do Sul. Quando comparados os modelos de UBS apenas os servi?os sem Sa?de da Fam?lia demonstraram alto grau de orienta??o ? APS para o Escore dos Atributos Essenciais e para o Escore Geral do PCATool-Brasil. Verificou-se que apesar de a ESF ser o meio pelo qual a APS reorganiza-se no Pa?s, esse modelo ainda n?o possui a efetividade planejada para a aten??o ? sa?de das pessoas idosas. Entende-se que os achados deste estudo contribuem para que novas estrat?gias no processo de trabalho da APS sejam pensadas de forma que acolham com mais universalidade as pessoas idosas e suas diferentes capacidades, incluindo aquelas mais longevas.