Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal.

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal

Since before the Neolithic Revolution, when human civilisation first emerged, humans and canines have lived, and died, together. This Scottish study is conducted in the field of animal-human interaction and, using qualitative methods, applies established insights from the sociology of health (born of human-to-human interaction) to a human-animal relationship. Specifically, this thesis explores death and dying in relations between the companion canines, and the human members, of ten families. Nonhuman illness narratives are found in profusion in this study, and it was also found to be possible to apply biographical disruption to nonhumans, when conceptualised as biographical disruption-by-proxy. Unexpectedly, there emerged from the data support for a four-fold model of canine selfhood, as forged within the family. This is, as far as I am aware, the first modelling of a specific nonhuman consciousness, within the discipline. Suffering was found to exist in both physical and non-physical forms for the companions, and a mutual vulnerability to loneliness, and desire for companionship, appears to be a powerful point of connection between the humans and the canines. Being together emerged as both a practice, and as an ideal, that moulded the human-canine relations, and it was regarded as unfitting for a canine to die alone. Companion canine dying comes forth as a negotiated process, shaped by a divide between gradual and sudden death. This work encountered developed narratives of departure, that seem to structure the experience of losing a companion. In particular the role of the expert is a privileged voice in the negotiations of dying, and the biomedical view is treated as being definitive. The role of the expert is not simply submitted to however, but a range of stances to veterinary authority are displayed, being; acquiescence, resistance and invalidation of the veterinary voice. Ultimately, whilst interplays of wellbeing are present, they are less biophysically grounded, than they are rooted in the everyday routines of life, in the rituals of eating, sleeping, walking, and playing together, that compose the shared world of the human and companion canine.

Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2014-09-01T15:25:12Z No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Dissertacao Gabriel Costa Jacobina.pdf: 1908943 bytes, checksum: 3486e27b8203b6a2af14a0943ee6bde1 (MD5) / Made available in DSpace on 2014-09-01T15:25:12Z (GMT). No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Dissertacao Gabriel Costa Jacobina.pdf: 1908943 bytes, checksum: 3486e27b8203b6a2af14a0943ee6bde1 (MD5) Previous issue date: 2012-08-29 / The ambulatory electrocardiography (ECG-Holter) is a method to obtain the electrocardiographic tracing continuously, noninvasively for prolonged periods (24 hours). Changes in cardiac conduction and arrhythmias can be observed frequently during the perioperative period (pre, during, and post), however this tends to be more careful monitoring before and during surgery. It was shown that there is always the risk of arrhythmias in the postoperative period, but there are few studies in dogs detecting and comparing the electrocardiographic changes during the perioperative period. In this work, we intend to perform a more comprehensive evaluation by electrocardiographic monitoring during the periods before, during and post-anesthesia using the electrocardiogram continuous 24 hours (ECG-Holter) in dogs. It were used 23 dogs underwent to elective surgical procedures and allocated into three groups, young dogs or young adults (GAS), dogs with cardiac disease (GCP) and older dogs (GAI). There were no clinically significant changes in the variables evaluated during the trans-anesthetic. There were no statistical differences between the quantities of arrhythmias observed in the three groups and at different times. However, a greatest number of EV EVS were observed in GAI, especially in the post-anesthetic evaluation. A dog of GAI showed large amounts of ventricular arrhythmias caused by a hidden heart disease. There was no significant difference between the FC (max, min and mean) and HRV between the groups. The atrioventricular blocks (1 and 2) occurred in some dogs in three groups and were mainly observed at night in the pre-and post-anesthetic evaluations and few minutes after anesthetic induction. In conclusion, the anesthetic protocols used proved to be safe and cause few complications in dogs during the trans-anesthetic ECG-Holter monitoring proved to be practical, easy to use, and important for the perianesthetic evaluation in dogs. / Com a eletrocardiografia é possível identificar alterações na condução cardíaca e arritmias, podendo também ser sugeridos aumentos de área cardíaca e distúrbios eletrolíticos. A eletrocardiografia contínua (ECG-Holter) é um método para se obter o traçado eletrocardiográfico de forma contínua, não invasiva, por períodos prolongados (24 horas). Alterações na condução cardíaca e arritmias podem ser observadas com frequência durante todo o período perioperatório (pré, trans, e pós), no entanto esta monitoração tende ser mais criteriosa antes e durante o procedimento cirúrgico. Foi demonstrado que há sempre o risco de desenvolvimento de arritmias no período pós-operatório, porém existem poucos estudos em cães detectando e comparando as alterações eletrocardiográficas durante todo o período perioperatório. Neste trabalho, foi realizada uma avaliação mais ampla, pela monitoração eletrocardiográfica durante os períodos pré, trans e pós-anestésico utilizando o eletrocardiograma contínuo 24 horas (ECG-Holter) em cães. Foram utilizados 23 cães submetidos a procedimentos cirúrgicos eletivos. Os animais foram distribuídos em três grupos: cães jovens ou adultos jovens saudáveis (GAS), cães com doença mixomatosa da valva mitral, (GCP) e cães idosos (GAI). O protocolo anestésico teve como base a pré-medicação com acepromazina e tramadol, meperidina ou morfina, indução com propofol e midazolam e a manutenção com isoflurano. Não houve diferenças estatísticas entre a quantidade de arritmias observadas nos três grupos, contudo o GAI demonstrou maior número de EVS e EV. Um cão do GAI apresentou grandes quantidades de arritmias ventriculares devido à uma cardiopatia oculta. Não houve diferença significativa entre as FC (max, mín e média) e nem na VFC entre os grupos. Bloqueios atrioventriculares (1o e 2º graus) que ocorreram em alguns cães foram observados principalmente nos momentos noturnos e no período trans-anestésico, alguns minutos após a indução. O método ECG-Holter demonstrou ser fundamental na detecção de arritmias em cães durante o período perianestésico, principalmente em cães assintomáticos. Os protocolos utilizados foram considerados seguros, com mínimas complicações no período trans-anestésico.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-09-13T21:42:04Z No. of bitstreams: 1 2017 - Andr? William Masseaux Vidal J?nior.pdf: 1275550 bytes, checksum: d2d211215832b8ab2e53ab8d6da5ae4b (MD5) / Made available in DSpace on 2018-09-13T21:42:04Z (GMT). No. of bitstreams: 1 2017 - Andr? William Masseaux Vidal J?nior.pdf: 1275550 bytes, checksum: d2d211215832b8ab2e53ab8d6da5ae4b (MD5) Previous issue date: 2017-02-15 / It was proposed by this study to evaluate two protocols (PA and PB) to obtain autologous canine PRP, which is easy to perform in an ambulatory (semiautomatic method) and of good quality (platelet concentration, qualitative evaluation of platelet morphology and low contamination with Erythrocytes), to later propose different therapeutic indications of these PRPs as tissue modulating agents, according to the observed cellular leukocyte pattern. For this purpose, 20 dogs (Canis lupus familiaris) were used at the UFRRJ (HV) Veterinary Hospital, males and females, aged between 1 and 7 years (mean 4 years), considered clinically and hematologically healthy for elective surgeries and / or routine consultations. After adequate trichotomy and antisepsis, 8 mL of blood were collected by venipuncture of the jugular, being immediately packed in two 4 mL flasks, vacuntainer type, containing sodium citrate 3,2%. Protocol A using double centrifugation with 210 xG and 370 xG and protocol B using double centrifugation with 140 x G and 330 x G. PRP samples obtained from each protocol were used to count platelets, erythrocytes and leukocytes in the Neubauer chamber, differential leukocyte counting and observation of platelet morphology in smears. Data (mean and standard deviations) were analyzed by the 95% probability t test (p <0,05) using Pearson's correlation to test the relationship between platelet and erythrocyte counts, platelets and leukocytes and leukocytes in Relation to red blood cells. There was a very weak negative correlation between platelets and leukocytes (? = -0,03), weak negative between platelets and erythrocytes (? = -0,3) and strong positive correlation between leukocytes and erythrocytes (? = 0,75). Although Protocol B did not reach the desired one million platelets average (979300 ? 79631 cells / ?L), both protocols, A and B (4,42 ? 1,61 and 3,85 ? 1,55 times more platelets than Total blood, respectively) (p <0,05) were efficient in concentrating platelets. The cytoplasmic prolongations evidencing platelet activation were present in 26,55 ? 6,72% of platelets of protocol A and 26,25 ? 7,03% in those of protocol B (p> 0,05). A and B presented a small number of red blood cells (p> 0,05), which were considered to be contaminants of the samples and, for the quantity of leukocytes, protocol A presented more white blood cells (p <0,05) than protocol B with higher concentrations of basophils , and lymphocytes. / Foi proposto por esse estudo avaliar dois protocolos (PA e PB) para obten??o de PRP canino aut?logo, de f?cil execu??o em ambulat?rio (m?todo semiautom?tico) e de boa qualidade (capacidade de concentra??o de plaquetas, avalia??o qualitativa da morfologia das plaquetas e reduzida contamina??o com eritr?citos), para posteriormente propor diferentes indica??es terap?uticas desses PRP como agentes moduladores de recupera??o tecidual, de acordo com o padr?o celular leucocit?rio observado. Para isso foram utilizados 20 c?es (Canis lupus familiaris) atendidos no Hospital Veterin?rio da UFRRJ (HV), machos e f?meas, com idade variando entre 1 e 7 anos (m?dia 4 anos), considerados saud?veis clinica e hematologicamente que se destinavam para cirurgias eletivas e/ou consultas de rotina. Ap?s tricotomia e antissepsia adequadas, eram coletados 8 mL de sangue por venopun??o da jugular sendo imediatamente acondicionados em dois frascos de 4 mL, do tipo vacuntainer, contendo citrato de s?dio a 3,2%. O protocolo A utilizando centrifuga??o dupla com 210 xG e 370 xG e protocolo B utilizando centrifuga??o dupla com 140 xG e 330 xG. Amostras de PRP obtidas a partir de cada protocolo foram destinadas a contagem de plaquetas, hem?cias e leuc?citos em c?mara de Neubauer, contagem diferencial dos leuc?citos e observa??o da morfologia das plaquetas em esfrega?os. Analisou-se os dados (m?dias e desvios padr?o) pelo Teste t com 95% de probabilidade (p<0,05) utilizando-se correla??o de Pearson para testar a rela??o entre a contagem de plaquetas e hem?cias, plaquetas e leuc?citos e leuc?citos em rela??o as hem?cias. Houve correla??o negativa muito fraca entre plaquetas e leuc?citos (?= -0,03), negativa fraca entre plaquetas e hem?cias (?= -0,3) e correla??o positiva forte entre leuc?citos e hem?cias (?=0,75). Embora o protocolo B n?o tenha alcan?ando a m?dia um milh?o de plaquetas desejado (979300 ? 79631 c?lulas/?L), ambos os protocolos, A e B (4,42 ? 1,61 e 3,85 ? 1,55 vezes mais plaquetas que o sangue total, respectivamente) (p<0,05) foram eficientes em concentrar plaquetas. Os prolongamentos citoplasm?ticos evidenciando ativa??o plaquet?ria estiveram presentes em 26,55 ? 6,72 % das plaquetas do protocolo A e 26,25 ? 7,03 % nas do protocolo B (p>0,05). PA e PB apresentaram reduzido n?mero de hem?cias (p>0,05) consideradas contaminantes das amostras e, quanto a quantidade de leuc?citos, o protocolo A apresentou mais gl?bulos brancos (p<0,05) que o protocolo B com maiores concentra??es de bas?filos, segmentados e linf?citos.

Made available in DSpace on 2014-08-20T14:37:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-06-23 / Mastocitomas are most prevalent cutaneous neoplasms in canines. Aiming to study the biologic behavior of the mastocytomas, an analysis was carried through retrospect of the diagnostic of neoplasms of skin in canines, obtained from biopsies and autopsies sent to the Regional Diagnostic Laboratory (LRD) of the Federal University of Pelotas (UFPEL), between January of 1986 and December of 2005. From this revision two approaches had been carried. An epidemiologic search and another morphologic carried through the rescued diagnostic of mastocitomas in the related period. To an epidemiologic approach, 80 samples of Mastocitomas were used. It was observed that 45 (56.2%) occurred in females, 33 (41.2%) in males and in 2 (2.5%) this data was not informed. These tumors occurred in 16 different breeds. Mastocytomas predominate in animals with 6-10-years-old. Only 25 samples could be used in the morphologic and morphometric evaluation. The neoplasms were processed and histologic slides were stained by Hematoxilin-eosin, Toluidin Blue and Masson trichromic. Cellular proliferation index was inferred with AgNOR. Based on these criteria, mastocitomas were graded. Seven (28%) were classified as grade I, 11 (44%) as grade II and 7 (28%) as grade III. In the morphometric study, mast cells cellular area and perimeter were measured. In order to obtain an improvement in the graduate quality its necessary the association of morphologic and morphometric parameters. / Mastocitomas estão entre os neoplasmas cutâneos caninos mais freqüentes. Com o objetivo de estudar o comportamento biológico desses tumores e de buscar métodos que permitam uma classificação mais objetiva e fidedigna, foi realizada uma análise retrospectiva dos Mastocitomas em caninos, provenientes de biópsias e necropsias enviadas ao Laboratório Regional de Diagnóstico (LRD) da Universidade Federal de Pelotas (UFPEL), no período de janeiro de 1986 a dezembro de 2005. A partir desta revisão realizou-se uma abordagem epidemiológica baseada nos dados de 80 mastocitomas diagnosticados no período. Assim sendo, verificou-se que quanto ao sexo, 45 (56,2%) foram diagnosticados em fêmeas, 33 (41,2%) em machos e para 2 (2,5%) animais o sexo não foi informado. Estes tumores foram diagnosticados em 16 raças diferentes, tendo predominado em cães sem raça definida e na raça Boxer. Os mastocitomas predominaram na faixa etária de 6-10 anos. Realizou-se, também, uma avaliação morfológica e morfométrica de parâmetros histológicos de 25 mastocitomas resgatados no período. As amostras foram classificadas de acordo com seu grau histológico e para tal foram utilizadas as colorações de hematoxilina e eosina, azul de toluidina e tricrômico de Masson e avaliou-se o índice de proliferação celular através da impregnação argêntica (AgNOR). Com base em critérios pré-definidos os 25 mastocitomas do estudo foram classificados em graus, obtendo-se sete (28%) classificados como grau I, 11(44%) como grau II e sete (28%) como grau III. Para o estudo morfométrico foram avaliados área e perímetro da célula e área e perímetro do núcleo dos mastócitos tumorais. Parâmetros morfométricos aliados aos parâmetros morfológicos de graduação dos mastocitomas conferiram maior confiabilidade ao diagnóstico.

Das canine Parvovirus (CPV-2)wurde erstmals bei Hunden im Jahr 1978 beschrieben. Dieses Virus verbreitete sich innerhalb weniger Monate weltweit in einer schweren Pandemie. Retrospektiv wird angegnommen, dass es sich aud dem Felinen Panleukopenie-Virus oder einem nah verwandten Virus entwickelt hat. Ziel dieser Arbeit war es, durch Untersuchungen von archivierten paraffineingebetteten Gewebeproben von Hunden und Katzen aus den frühen 1970iger Jahren einen möglichen Anzestor des caninen Parvovirus zu identifizieren und die Hypothese zu überprüfen, dass CPV-2 schon vor 1978 in den Hundepopulationen zirkulierte.

<p>  </p> <p>Adoptive cell transfer of chimeric antigen receptors (CAR) T cells has successfully targeted hematological malignancies in human patients. However, unpredicted side effects experienced after injection of the CAR T cells suggests the need for an optimal predictive preclinical animal model. Dogs have intact immune systems and develop solid tumors spontaneously with similar morphology and genetics to humans. I hypothesize that generating CAR T cells for dogs will closely mimic human patients' outcomes, thus providing new understandings of the safety of this immunotherapy. In addition to the dog as a preclinical model, we propose using a universal CAR T cell to overcome various tumor-related immunosuppressive challenges and control the killing of the target cells. To achieve this, we established methods for activating and expanding canine T cells to a clinically relevant scale. Then, we expressed a second-generation anti-FITC-8-41BB-ζ CAR T cell via lentiviral transduction. In the presence of the correct low-molecular-weight bispecific adapter, we showed <em>in-vitro</em> CAR-mediated function. Our results proved that it is feasible to generate functional canine anti-FITC-8-BB-ζ CAR T cells for therapy.</p>

Altersassoziiert entwickeln Hunde eine Erkrankung, die in vielen Aspekten der Alzheimer-Krankheit des Menschen ähnelt. Das canine kognitive Dysfunktionssyndrom äußert sich klinisch u.a. durch Desorientierung in vertrauter Umgebung, Vergessen von Kommandos und einen gestörten Schlaf-Wach-Rhythmus. Aus der Literatur ist bekannt, dass in den Gehirnen von alten Hunden regelmäßig Aβ- und selten Tauablagerungen zu beobachten sind. Allerdings erfolgte bisher kein Nachweis des hochgradig zytotoxischen und modifizierten pE3Aβ. Auch Veränderungen der mikroglialen Morphologie wurden bisher nicht beschrieben. Insgesamt lagen in dieser Studie 24 euthanasierte Rasse- und Mischlingshunde verschiedenen Alters vor. Fünf dieser Tiere besaßen ein durchschnittliches Alter von 2,1 Jahren und dienten als Kontrollgruppe. Die anderen 19 Hunde waren 8 bis 19 Jahre alt und wurden entsprechend ihrer Größe und des Gewichts in die drei Kategorien kleine (≤ 10 kg), mittelgroße (10 – 25 kg) und große Hunde (> 25 kg) unterteilt. Die Gehirne wurden aus den Schädeln präpariert und in 4 % Paraformaldehyd fixiert. Anschließend erfolgte die Präparation des frontalen und entorhinalen Kortex sowie der Hippokampusformation, die in 30%iger Saccharoselösung vitrifiziert und mittels Methylbutan bei -80 °C eingefroren wurden. Von den Regionen wurden Kryoschnitte mit einer Dicke von 40 µm angefertigt und diese anhand immunhistologischer Färbungen auf das Vorhandensein von Ablagerungen, bestehend aus den Amyloidsubtypen Aβ8-17 und pE3Aβ, sowie aus hyperphosphorylierten Tau, untersucht. Die Morphologie und das Aktivitätsstadium der Mikroglia wurden mit Antikörpern gegen Iba1 und TAL.1B5 analysiert. Zusätzlich erfolgte eine Untersuchung anhand des Filament Tracer. Stereologische Analysemethoden wurden zur Quantifizierung der Aβ-Ablagerungen und der Mikroglia angewandt. Disseminierte Plaques fanden sich bereits ab 9 Jahren. In den untersuchten Gehirnregionen von alten Hunden zeichnete sich ein progressiver Verlauf der Ablagerungen ab. Da insbesondere kleinere Hunde ein höheres Alter erreichten als mittelgroße und große Hunde konnten in dieser Kategorie vermehrt Plaques beobachtet werden. Den alten Tieren gemein war, dass in den untersuchten Gehirnregionen pE3Aβ-Plaques häufiger vorlagen als Plaques, die aus Aβ8-17 bestanden. Kleinere parenchymale und meningeale Gefäße des frontalen Kortex schienen besonders anfällig gegenüber pE3Aβ-Ablagerungen zu sein. Im entorhinalen Kortex von kleinen Hunden war die Menge an gefäßassoziierten Aβ8-17- und pE3Aβ-Ablagerungen annähernd gleich. Bei mittelgroßen und großen Hunden dominierte im entorhinalen Kortex und ventralen Hippokampus die Anzahl an gefäßassoziierten Aβ8-17-Ablagerungen. Bei kleinen Hunden existierten im ventralen Hippokampus signifikant mehr gefäßassoziierte Aβ8-17- als pE3Aβ-Ablagerungen. Hyperphosphoryliertes Tau fand sich in der Hippokampusformation von drei Hunden im Alter von 11 bzw. 15 Jahren. Der Schweregrad war unterschiedlich ausgeprägt, sodass nur ein Hund eine hochgradige Pathologie mit NFTs und neuritischen Plaques aufwies. Einhergehend mit dem Alter und einer assoziierten Proteinpathologie fanden sich Veränderungen der mikroglialen Morphologie. Neben ramifizierten Mikroglia lagen in den untersuchten Gehirnregionen aktivierte Mikroglia vor. Einige Mikroglia wiesen Zeichen einer Seneszenz auf und waren insbesondere in den Gehirnen von Hunden mit einer hochgradigen Aβ- bzw. Tau-Pathologie vorhanden. Zusammenfassend ist festzustellen, dass mit dieser Studie eine nähere Charakterisierung des caninen kognitiven Dysfunktionssyndroms erfolgte. Die Befunde sind von hoher translationaler Bedeutung und fördern die Etablierung des Hundes als natürliches Modelltier zur Untersuchung von Alterungsprozessen des Gehirns und für die Erforschung des initialen Stadiums der Alzheimer-Krankheit. / Dogs develop an age-associated cognitive dysfunction syndrome with several aspects resembling Alzheimer\\\'s disease. Affected animals show signs of dis-orientation in their familiar surroundings, dementia, and a disturbed circadian rhythm. The underlying neurodegenerative disease is associated with patho-logic changes in the brain including regularly deposition of β-pleated amyloid and rarely hyperphosphorylated tau accumulation. However, there have been no reports of the highly cytotoxic and modified pE3Aβ in the canine brain. Equally, altered microglial morphology has not been documented so far. For this study 24 euthanized thoroughbred dogs and mongrels of different ages were available. Five of these animals had an average age of 2.1 years and served as control group. The remaining 19 dogs were 8 to 19 years old. Accor-ding to their height and weight these dogs were divided into 3 different categories including small (≤ 10 kg), medium (11 - 25 kg) and large dogs (> 25 kg). Brains were dissected from the skulls and fixed in 4 % paraformaldehyde. Afterwards the frontal and entorhinal cortex as well as the hippocampal for-mation were isolated, vitrificated in 30 % sucrose solution and frozen to -80 °C by methylbutane. These regions were sliced into 40 µm thick sections and subsequently stained by immunohistology in order to detect deposits of Aβ8-17, pE3Aβ and hyperphosphorylated tau, respectively. Antibodies against Iba1 and TAL.1B5 were used to analyze microglial morphology and activation status. Additionally further investigations were made with the Filament Tracer of Imaris software. Stereological analysis methods served for the quantification of Aβ depositions and microglia. Disseminated Aβ plaques were detected in dogs from 9 years on. Within the examined brain regions of elderly dogs a progressive course of Aβ depositions was observed. Especially small dogs had a longer lifespan than medium and large dogs with the result that more plaques were deposited in the brains of small dogs. Elderly dogs had in common that pE3Aβ-plaques where more often located in the examined brain regions than plaques containing Aβ8-17. Minor parenchymal and meningeal vessels seemed to be susceptible especially to pE3Aβ depositions. The amount of vessel-associated Aβ8-17 and pE3Aβ in the entorhinal cortex of small dogs was almost equal. Within the entorhinal cortex of medium and large dogs the amount of vessel-associated Aβ8-17 predominated. The ventral hippocampus of small dogs showed significantly more vessel-associated Aβ8-17 than pE3Aβ depositions. Hyperphosphorylated tau was present in the hippocampal formations of 3 dogs with an age of 11 and 15 years, respectively. The degree of severity varied with the result that only one dog showed a high-grade pathology with development of NFTs and neuritic plaques. Accompanied by age and associated protein pathology altered microglial morphology was detected. Alongside with ramified microglia, activated cells were identified in the examined brain regions. Several microglia showed signs of senescence and were present in the brains of dogs with severe Aβ and tau pathology. Summarizing, this study facilitated a further characterization of the canine cognitive dysfunction syndrome. The results are of highly translational importance and encourage the establishment of the dog as a natural animal model for studying age-associated processes and the initial stage of Alzheimer’s disease.

Auf dem Gebiet der Veterinärmedizin wird in Deutschland die Desinfektionsmittelprüfung nach den Richtlinien der Deutschen Veterinärmedizinischen Gesellschaft e.V. (DVG) durchgeführt. Diese Richtlinien realisieren derzeit eine Viruzidieprüfung nur für den Bereich Tierhaltung. Daher wurde in dieser Arbeit eine praxisnahe Methodik zur Prüfung der viruziden Wirksamkeit von Desinfektionsmitteln für den Bereich der Lebensmittelproduktion und der tierärztlichen Praxis entwickelt. Neben dem Einsatz von zwei relevanten Prüfviren, erfolgte die Prüfung der viruziden Wirksamkeit anhand fünf verschiedener chemischer Grundsubstanzen. Um praxisähnliche Bedingungen zu simulieren, wurden unterschiedliche Belastungssubstanzen und Prüftemperaturen zur Testung herangezogen. Die gewonnenen Erkenntnisse können somit auf dem Gebiet der Viruzidieprüfung in zukünftige Neufassungen der DVG-Richtlinie berücksichtigt werden.