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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
111

Functional Characterization of a Cathepsin L in Drosophila Melanogaster

Dong, Qian 01 July 2015 (has links)
The Drosophila dorsal Air Sac Primordium (ASP) is a tracheal tube that invasively grows toward and into the wing imaginal disc. The unfolding of Drosophila wing is a process following eclosion with a cuticular bilayer replacing epithelial cells originally packing the wing. We reasoned that protease functions might be needed for the invasion of ASP into the wing imaginal disc as well as the rearrangement of epithelia cells during wing unfolding. Our study is particularly focused on understanding the role of a Cathepsin L like cysteine protease (CP1) in the development of dorsal ASP and wing development of Drosophila melanogaster. To analyze the function of CP1, we overexpressed and knocked down CP1, respectively, using UAS-GAL4 system in combination with RNA interference technology. We found that both the knockdown and overexpression of CP1 in ASP resulted in perturbed growth, migration and weakened invasion of ASPs. We further explored the mechanism by which CP1 regulates ASP development and found that CP1 is capable of degrading collagen IV, a component of extracellular matrix. For wing development, we observed that both the knockdown and overexpression of CP1 in wing imaginal discs interrupted with normal wing development. In summary, our study demonstrated that CP1 facilitates the normal development of ASPs by degrading extracellular matrix and regulates wing development via a complex network of signaling pathways and protein interactions. Knowledge gained from this study has the potential to help us better understand the invasion of tumor cells through the extracellular matrix in humans.
112

A study of proteinases of invasive cells using cryoultramicrotomy and immunogold labelling.

Elliott, Edith. January 1993 (has links)
This study forms part of an investigation into the possible relevance of the lysosomal proteinases, cathepsins B, H, Land D, in cancer cell invasion. In this study, the main technique adopted was the Tokuyasu "cryo" method, in which the tissues were fixed, frozen and sectioned and labelled using the relevant antibodies, which were detected with protein A gold probes. In order to implement the Tokuyasu technique, it was necessary to rebuild a knife maker, for the production of adequately sharp glass knives, and to modify a sputter-coater into a glow-discharger, for rendering carbon-coated grids hydrophilic, to promote adhesion of hydrated sections. This study was directed towards human tissues and peptide antibodies were investigated as a means of avoiding isolation of proteins from scarce human tissue, and as a means of obtaining antibodies that will target specific regions of proteins of interest. Peptide antibodies were also considered promising for studies of proteinase trafficking and as immunoinhibiting agents, potentially useful in cancer therapy. Various prediction programmes were investigated for their effectiveness in predicting whether a given peptide sequence will elicit antibodies that will react with the native protein. Successful prediction would increase the success rate of peptide antibody production and thus lower the cost. Leucocytes were studied as a model of an invasive cell, since they are more readily available than tumour cells and serve the purpose during the development of methods. In the course of these studies, an optimal protocol for the fixation of PMNs was developed, involving lateral fixation of cut sections, that should be useful for future studies on these cells. Elastase and cathepsins D and G were found on the surface of activated PMNs and could thus play a role in the invasive properties of these cells. Studies on MCF-10A "normal" breast epithelial cells and their ras-transformed Neo-T counterparts revealed that upon transformation, lysosomes shift from a perinuclear position, to a more peripheral position. None of the cathepsins studied was found on the cell surface of either the normal or ras-transfected cells, suggesting that surface distribution of these enzymes may not be a requirement for invasiveness. These studies suggest that immunocytochemical investigation of cells, in the process of invading through a barrier membrane, might be profitable in elucidating the role of proteinases in invasive cancer. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 1993.
113

The Design, Synthesis and Biological Assay of Cysteine Protease Specific Inhibitors

Mehrtens (nee Nikkel), Janna Marie January 2007 (has links)
This thesis investigates the design, synthesis and biological assay of cysteine protease inhibitors within the papain superfamily of cysteine proteases. This is achieved by examining the effect of inhibitor design, especially warheads, on IC₅₀ values and structureactivity relationships between cysteine protease inhibitors of the papain superfamily. The representative proteases used are m-calpain, μ-calpain, cathepsin B and papain. Chapter One is an introductory chapter; Chapters Two-Four describe the design and synthesis of cysteine protease inhibitors; Chapter Five discusses assay protocol; and Chapter Six contains the assay results and structure-activity relationships of the synthesised inhibitors. Chapter One introduces cysteine proteases of the papain family and examines the structure, physiology and role in disease of papain, cathepsin B, m-calpain and μ-calpain. The close structural homology that exists between these members of the papain superfamily is identified, as well characteristics unique to each protease. Covalent reversible, covalent irreversible and non-covalent warheads are defined. The generic inhibitor scaffold of address region, recognition and warhead, upon which the inhibitors synthesised in this thesis are based, is also introduced. Chapter Two introduces reversible cysteine protease inhibitors found in the literature and that little is known about the effect of inhibitor warhead on selectivity within the papain superfamily. Oxidation of the dipeptidyl alcohols 2.6, 2.26, 2.29, 2.30, 2.35 and 2.36 utilising the sulfur trioxide-pyridine complex gave the aldehydes 2.3, 2.27, 2.19, 2.2, 2.21 and 2.22. Semicarbazones 2.37-2.40 were synthesised by a condensation reaction between the alcohol 2.3 and four available semicarbazides. The amidoximes 2.48 and 2.49 separately underwent thermal intramolecular cyclodehydration to give the 3-methyl-1,2,4- oxadiazoles 2.41 and 2.50. The aldehydes 2.3 and 2.27 were reacted with potassium cyanide to give the cyanohydrins 2.51 and 2.52. The cyanohydrins 2.51 and 2.52 were separately reacted to give 1) the α-ketotetrazoles 2.43 and 2.55; 2) the α-ketooxazolines 2.42 and 2.58; 3) the esterified cyanohydrins 2.60 and 2.61. A two step SN2 displacement reaction of the alcohol 2.6 to give the azide 2.62, an example of a non-covalent cysteine protease inhibitor. Chapter Three introduces inhibitors with irreversible warheads. The well-known examples of epoxysuccinic acids 3.1 and 3.5 are discussed in detail, highlighting the lack of irreversible cysteine protease specific inhibitors. The aldehydes 2.3 and 2.27 were reacted under Wittig conditions to give the α,β-unsaturated carbonyls 3.14-3.18. Horner- Emmons-Wadsworth methodology was utilised for the synthesis of the vinyl sulfones 3.20- 3.23. The dipeptidyl acids 2.24 and 2.28 were separately reacted with diazomethane to give the diazoketones 3.25 and 3.26. The diazoketones 3.25 and 3.26 were separately reacted with hydrogen bromide in acetic acid (33%) to give the α-bromomethyl ketones 3.27 and 3.28, which were subsequently reduced to give the α-bromomethyl alcohols 3.29-3.32. Under basic conditions the α-bromomethyl alcohols 3.29-3.32 ring-closed to form the peptidyl epoxides 3.33-3.36. Chapter Four introduces the disadvantages of peptide-based inhibitors. A discussion is given on the benefits of constraining inhibitors into the extended bioactive conformation known as a β-strand. Ring closing metathesis is utilised in the synthesis of the macrocyclic aldehyde 4.4, macrocyclic semicarbazone 4.15, the macrocyclic cyanohydrin 4.16, the macrocyclic α-ketotetrazole 4.18 and the macrocyclic azide 4.19. Chapter Five introduces enzyme inhibition studies. The BODIPY-casein fluorogenic assay used for establishing inhibitor potency against m-calpain and μ-calpain is validated. Assay protocols are also established and validated for cathepsin B, papain, pepsin and α- chymotrypsin. A discussion of the effect of solvent on enzyme activity is also included as part of this study. Chapter Six presents the assay results for all the inhibitors synthesised throughout this thesis and an extensive structure-activity relationship study between inhibitors is included. The alcohols 2.26 and 2.30 are unprecedented examples of non-covalent, potent, cathepsin B inhibitors (IC₅₀ = 0.075 μM selectivity 80-fold and 1.1 μM, selectivity 18-fold). The macrocyclic semicarbazone 4.15 is an unprecedented example of a potent macrocyclic cysteine protease inhibitor (m-calpain: IC₅₀ = 0.16 μM, selectivity 8-fold). The cyanohydrin 2.51 contains an unprecedented cysteine protease warhead and is a potent and selective inhibitor of papain (IC₅₀ = 0.030 μM, selectivity 3-fold). The O-protected cyanohydrin 2.61 is a potent and selective inhibitor of pepsin (IC₅₀ = 1.6 μM, selectivity 1.5-fold). The top ten warheads for potent, selective cathepsin B inhibition are: carboxylic acid, methyl ester, diazoketone, esterified cyanohydrin, α-bromomethyl ketone, α,β- unsaturated aldehyde, vinyl sulfones, α-bromomethyl-C₃-S,R-alcohol, alcohol and α,β- unsaturated ethyl ester. The selectivity of these warheads was between 5- and 130-fold for cathepsin B. The best inhibitors for cathepsin B were the α-bromomethyl ketone 3.26 (IC₅₀ = 0.075 μM, selectivity 16-fold), the α,β-unsaturated aldehyde 3.18 (IC₅₀ = 0.13 μM, selectivity 13-fold) and the esterified cyanohydrin 3.59 (IC₅₀ = 0.35 μM, selectivity 22- fold). Chapter Seven outlines the experimental details and synthesis of the compounds prepared in this thesis.
114

The Design, Synthesis and Biological Assay of Cysteine Protease Specific Inhibitors

Mehrtens (nee Nikkel), Janna Marie January 2007 (has links)
This thesis investigates the design, synthesis and biological assay of cysteine protease inhibitors within the papain superfamily of cysteine proteases. This is achieved by examining the effect of inhibitor design, especially warheads, on IC₅₀ values and structureactivity relationships between cysteine protease inhibitors of the papain superfamily. The representative proteases used are m-calpain, μ-calpain, cathepsin B and papain. Chapter One is an introductory chapter; Chapters Two-Four describe the design and synthesis of cysteine protease inhibitors; Chapter Five discusses assay protocol; and Chapter Six contains the assay results and structure-activity relationships of the synthesised inhibitors. Chapter One introduces cysteine proteases of the papain family and examines the structure, physiology and role in disease of papain, cathepsin B, m-calpain and μ-calpain. The close structural homology that exists between these members of the papain superfamily is identified, as well characteristics unique to each protease. Covalent reversible, covalent irreversible and non-covalent warheads are defined. The generic inhibitor scaffold of address region, recognition and warhead, upon which the inhibitors synthesised in this thesis are based, is also introduced. Chapter Two introduces reversible cysteine protease inhibitors found in the literature and that little is known about the effect of inhibitor warhead on selectivity within the papain superfamily. Oxidation of the dipeptidyl alcohols 2.6, 2.26, 2.29, 2.30, 2.35 and 2.36 utilising the sulfur trioxide-pyridine complex gave the aldehydes 2.3, 2.27, 2.19, 2.2, 2.21 and 2.22. Semicarbazones 2.37-2.40 were synthesised by a condensation reaction between the alcohol 2.3 and four available semicarbazides. The amidoximes 2.48 and 2.49 separately underwent thermal intramolecular cyclodehydration to give the 3-methyl-1,2,4- oxadiazoles 2.41 and 2.50. The aldehydes 2.3 and 2.27 were reacted with potassium cyanide to give the cyanohydrins 2.51 and 2.52. The cyanohydrins 2.51 and 2.52 were separately reacted to give 1) the α-ketotetrazoles 2.43 and 2.55; 2) the α-ketooxazolines 2.42 and 2.58; 3) the esterified cyanohydrins 2.60 and 2.61. A two step SN2 displacement reaction of the alcohol 2.6 to give the azide 2.62, an example of a non-covalent cysteine protease inhibitor. Chapter Three introduces inhibitors with irreversible warheads. The well-known examples of epoxysuccinic acids 3.1 and 3.5 are discussed in detail, highlighting the lack of irreversible cysteine protease specific inhibitors. The aldehydes 2.3 and 2.27 were reacted under Wittig conditions to give the α,β-unsaturated carbonyls 3.14-3.18. Horner- Emmons-Wadsworth methodology was utilised for the synthesis of the vinyl sulfones 3.20- 3.23. The dipeptidyl acids 2.24 and 2.28 were separately reacted with diazomethane to give the diazoketones 3.25 and 3.26. The diazoketones 3.25 and 3.26 were separately reacted with hydrogen bromide in acetic acid (33%) to give the α-bromomethyl ketones 3.27 and 3.28, which were subsequently reduced to give the α-bromomethyl alcohols 3.29-3.32. Under basic conditions the α-bromomethyl alcohols 3.29-3.32 ring-closed to form the peptidyl epoxides 3.33-3.36. Chapter Four introduces the disadvantages of peptide-based inhibitors. A discussion is given on the benefits of constraining inhibitors into the extended bioactive conformation known as a β-strand. Ring closing metathesis is utilised in the synthesis of the macrocyclic aldehyde 4.4, macrocyclic semicarbazone 4.15, the macrocyclic cyanohydrin 4.16, the macrocyclic α-ketotetrazole 4.18 and the macrocyclic azide 4.19. Chapter Five introduces enzyme inhibition studies. The BODIPY-casein fluorogenic assay used for establishing inhibitor potency against m-calpain and μ-calpain is validated. Assay protocols are also established and validated for cathepsin B, papain, pepsin and α- chymotrypsin. A discussion of the effect of solvent on enzyme activity is also included as part of this study. Chapter Six presents the assay results for all the inhibitors synthesised throughout this thesis and an extensive structure-activity relationship study between inhibitors is included. The alcohols 2.26 and 2.30 are unprecedented examples of non-covalent, potent, cathepsin B inhibitors (IC₅₀ = 0.075 μM selectivity 80-fold and 1.1 μM, selectivity 18-fold). The macrocyclic semicarbazone 4.15 is an unprecedented example of a potent macrocyclic cysteine protease inhibitor (m-calpain: IC₅₀ = 0.16 μM, selectivity 8-fold). The cyanohydrin 2.51 contains an unprecedented cysteine protease warhead and is a potent and selective inhibitor of papain (IC₅₀ = 0.030 μM, selectivity 3-fold). The O-protected cyanohydrin 2.61 is a potent and selective inhibitor of pepsin (IC₅₀ = 1.6 μM, selectivity 1.5-fold). The top ten warheads for potent, selective cathepsin B inhibition are: carboxylic acid, methyl ester, diazoketone, esterified cyanohydrin, α-bromomethyl ketone, α,β- unsaturated aldehyde, vinyl sulfones, α-bromomethyl-C₃-S,R-alcohol, alcohol and α,β- unsaturated ethyl ester. The selectivity of these warheads was between 5- and 130-fold for cathepsin B. The best inhibitors for cathepsin B were the α-bromomethyl ketone 3.26 (IC₅₀ = 0.075 μM, selectivity 16-fold), the α,β-unsaturated aldehyde 3.18 (IC₅₀ = 0.13 μM, selectivity 13-fold) and the esterified cyanohydrin 3.59 (IC₅₀ = 0.35 μM, selectivity 22- fold). Chapter Seven outlines the experimental details and synthesis of the compounds prepared in this thesis.
115

Biologia molecular aplicada à identificação de alvos para o controle do bicudo da cana‐de‐açúcar, Sphenophorus levis

Paula, Fernando Fonseca Pereira de 29 February 2012 (has links)
Made available in DSpace on 2016-06-02T20:20:35Z (GMT). No. of bitstreams: 1 5217.pdf: 18042962 bytes, checksum: 6c55d259639b3b244326b14d21f608f1 (MD5) Previous issue date: 2012-02-29 / Universidade Federal de Minas Gerais / The sugarcane weevil, Sphenophorus levis, is an insect that feeds on the rhizome of sugarcane in its larval stage, boring channels that cause damage and death to the plant. Conventional methods of insect control have not been efficient. The aim of the present study was to generate knowledge on the biology of this insect on the molecular level using a transcriptomic approach to determine potential targets genes for the engineering of insect-resistant plants. After sequence processing and assembly using the dCAS program, 3804 sequences were grouped into 201 contigs and 1363 singlets, which were manually annotated. Several plant cell wall degrading enzymes were identified, including pectinases and cellulases. An invertasecontaining contig was identified for the first time in a coleopteran. Total probable digestive enzymes accounted for 19.3% and unknown genes accounted for 28.8% of the total number of expressed sequence tags. Considering the predominance of cathepsin L enzymes among the digestive enzymes found in the transcriptome (54.3%) and their importance to the breakdown of proteins in the insect digestive process, a cDNA clone encoding a cathepsin L enzyme, denominated Sl-CathL, was chosen for recombinant expression in Pichia pastoris cells, characterization and in vitro inhibition by the sugarcane cystatin CaneCPI-4 (Ki = 0.196 nM). Immunolocalization assays demonstrated the production of Sl-CathL in the midgut epithelium and secretion into the gut lumen from vesicles containing the enzyme. S. levis demonstrated sensitivity in triggering RNA interference machinery induced by dsRNA injections in the body cavity and gene-specific knockdown was confirmed by qRT-PCR. Larvae injected with V-ATPase E dsRNA died within three weeks after injection and serpin 1-silenced larvae either exhibited delayed development, arresting in the pupal stage, or died as pharate adults. In conclusion, transcriptome analyses, together with the inhibition of the main digestive enzyme by Cane-CPI-4 and gene silencing using RNAi, are promising procedures for the development of transgenic sugarcane plants to enhance resistance to Sphenophorus levis. / O bicudo da cana-de-açúcar, Sphenophorus levis, é um inseto que na fase larval se alimenta do rizoma da cana-de-açúcar cavando galerias que causam danos e levam à morte das plantas. Os métodos convencionais de controle disponíveis não tem sido eficientes contra esse inseto. O objetivo desse estudo foi gerar conhecimento sobre a biologia desse inseto em nível molecular utilizando uma abordagem transcriptômica para a identificação de possíveis genes alvo, visando futuros estudos para desenvolvimento de plantas transgênicas resistentes ao ataque deste inseto. Após o processamento e montagem das sequências utilizando o programa dCAS, 3804 sequências foram agrupadas em 201 contigs e 1363 singlets, os quais foram manualmente anotados. Foram identificados diversos genes de degradação de parede celular, incluindo pectinases e celulases. Pela primeira vez um contig contendo uma sequência que codifica uma invertase foi identificado em um coleóptero. As prováveis enzimas digestivas totalizam 19,3% e os genes desconhecidos representam 28,8% do total de sequências expressas. Considerando a predominância de catepsinas L dentre as enzimas digestivas identificadas no transcriptoma (54,3%) e a sua importância para a hidrólise de proteínas nos processos digestivos, um clone de cDNA codificando uma catepsina L, denominado Sl-CathL, foi escolhido para a expressão recombinante em células de Pichia pastoris, caracterização e inibição in vitro utilizando a cistatina da cana-de-açúcar CaneCPI-4 (Ki = 0,196 nM). Os ensaios de imunolocalização evidenciaram a produção da Sl-CathL no epitélio do intestino médio e a secreção de vesículas, contendo a enzima, no lúmen do intestino. Larvas do inseto S. levis também apresentaram sensibilidade para disparar a maquinaria de RNA de interferência induzida por injeções de dsRNA na cavidade corpórea e o silenciamento gênico específico foi confirmado por qRT-PCR. As larvas que receberam injeções com dsRNA da V-ATPase E morreram dentro de três semanas, enquanto as larvas que tiveram a serpina 1 silenciada exibiram atraso no desenvolvimento, aprisionamento na fase pupal, ou morreram como adultos farados. Desse modo, concluímos neste trabalho iniciado a partir da análise do transcriptoma, que a inibição da principal enzima digestiva pela Cane-CPI-4 e o silenciamento gênico via RNAi são alternativas promissoras para o estabelecimento de plantas resistentes ao inseto e podem ser aplicadas no desenvolvimento de plantas de cana-de-açúcar transgênicas com o objetivo de aumentar sua resistência ao Sphenophorus levis.
116

Infection et stimulation de cellules endothéliales par Porphyromonas gingivalis et son lipopolysaccharide : lien entre maladies parodontales et athérosclérose / Infection and stimulation of endothelial cells with porphyromonas gingivalis and its lipopolysaccharide : link between periodontal diseases and atherosclerosis

Huck, Olivier 10 April 2013 (has links)
Depuis plusieurs années, l’influence des pathologies parodontales sur certaines pathologies systémiques, notamment les maladies cardio-vasculaires et l’athérosclérose apparait de plus en plus évidente. Dans notre étude, nous nous sommes intéressés à l’évaluation des effets induits par Porphyromonas gingivalis, une des principales bactéries parodontopathogènes, et son lipopolysaccharide sur les cellules endothéliales, qui forment une interface entre le flux sanguin et la paroi vasculaire, d’où un rôle important dans l’initiation et le développement de la plaque d’athérome. Nous avons surtout ciblé les effets induits sur la cathepsine B, une protéase impliquée dans le développement de la plaque d’athérome, et sur l’inflammasome, un complexe impliqué dans la production d’IL-1beta. Les résultats de nos travaux montrent que l’infection par Porphyromonas gingivalis et la stimulation par son LPS sont capables d’induire une augmentation de l’activité enzymatique de la cathepsine B, ceci suivant différentes cinétiques. Dans les deux cas, ces augmentations d’activité se font sans modifications de la synthèse d’ARNm, ni de la concentration protéique de l’enzyme. Nos résultats démontrent également que l’infection par Porphyromonas gingivalis entraine une augmentation de l’expression ARN de l’inflammasome NLRP3, mais celle ci n’est pas observée au niveau protéique du fait d’un processus de protéolyse de la protéine NLRP3 suite à l’infection. Dans un deuxième temps, nous avons développé un modèle de parodontite expérimentale, fiable et reproductible, nous permettant d’envisager une expérimentation in vivo afin d’observer les interactions à distance entre maladies parodontales et athérosclérose sur dessouris apolipoprotéine-E -/-. / Periodontal diseases have been linked to systemic diseases especially cardiovascular diseases and atherosclerosis. In our study, we investigated the effects induced by an infection with Porphyromonas gingivalis, a major periodontal pathogen, and stimulation by its lipopolysaccharide on endothelial cells at the interface between the inner part of arteries and blood flow. We focused on the effects induced on cathepsin B, a protease involved in atherosclerosis and on the activation of inflammasome, an intracellular complex linked to secretion of IL-1beta. Results showed that infection with Porphyromonas gingivalis and stimulation by its lipopolysaccharide increase enzymatic activity of cathepsin B with different kinetics. These modifications are observed without any modifications of RNAm expression and protein concentration. We also showed that infection with Porphyromonas gingivalis increases RNAm expression of NLRP3 but this increase at the RNAm level is not associated with an increase of the protein concentration due to an induced proteolysis. Furthermore, we developed a reliable model of experimental periodontitis that will be used to analyze interactions between periodontitis and systemic diseases in vivo, especially in apolipoprotein-E -/- mice.
117

Fisiologia molecular digestiva de Musca domestica (DIPTERA) / Molecular physiology of Musca domestica (Diptera)

Marcelo Henrique Peteres Padilha 13 November 2009 (has links)
A mosca domestica (Musca domestica) é um dos insetos largamente distribuído e conhecido pelo homem. A larva de M. domestica possui no conteúdo luminal do ventrículo anterior e médio uma atividade proteolítica com pH ótimo entre 3,0 3,5 e propriedades cinéticas similares a catepsina-D. Três cDNAs codificantes para preprocatepsina-D (ppCAD1, ppCAD2 e ppCAD3) foram clonados a partir de uma biblioteca de cDNA ventricular de larvas de M. domestica. As sequências possuem o peptídeo sinal, o propeptídeo e a enzima madura contendo os resíduos catalíticos e todos os resíduos de ligação ao substrato conservados, achados em uma catepsina-D lisossomal bovina. Um cladograma de sequências de aminoácidos de catepsinas-D de insetos e vertebrados depositados no GENBANK formou um grande grupo dividido em duas ramificações monofiléticas: Uma com sequências de vertebrados e a outra com sequências de catepsinas-D lisossomais de insetos incluindo a ppCAD1. A sequência do pepsinogênio humana, ppCAD2, ppCAD3 e uma sequência de D. melanogaster são excluídas desse grande grupo indicando uma função não lisossomal para essas sequências. ppCAD3 deve corresponder a uma catepsina-D digestiva encontrada no conteúdo luminal em larvas do inseto devido: (1) Análise por RT-PCR indicam que os transcritos codificantes para a CAD3 são expressos no ventrículo anterior e porção proximal do ventrículo médio. (2) pCAD3 recombinante após autoativação sob condições ácidas possui um pH ótimo entre 2,5 3,0 que é próximo ao pH luminal do ventrículo médio onde essa enzima atua. (3) Imunoblots das proteínas de diferentes tecidos e marcadas com o anticorpo preparado contra a pCAD3 foi positiva apenas nos tecidos e conteúdo do ventrículo anterior e médio. (4) CAD3 é localizada pela técnica de imuno-ouro no interior de vesículas de secreção e próxima a microvilosidades nas células do ventrículo anterior e médio. Esses dados suportam a idéia que ao se adaptar a um hábito detritivo, a CAD digestiva da mosca (e de outros Diptera Cyclorrhapha) resultou do mesmo gene ancestral da catepsina-D lisossomal intracelular, da mesma forma que se acredita que ocorreu com a pepsina em vertebrados. Uma limitada quantidade de informações de sequências de DNA e aspectos moleculares de M. domestica é disponível até o presente momento. Nós então propusemos gerar sequências de ESTs a partir de uma bibilioteca de cDNA ventricular da larva desse inseto. Um total de 826 ESTs randomicamente selecionados presentes no ventrículos de larvas de M. domestica foram seqüenciados e analisados com programas de bioinformática e separado em 323 clusters. As sequências foram manualmente anotadas e separadas em 3 categorias: (S) produtos provavelmente secretados, (H) produtos de metabolismo em geral (housekeeping) e (U) produtos sem função conhecida. Cento e sessenta clusters (423 ESTs) codificavam para proteínas secretadas tais como: lisozimas, lipases, tripsinas, quimotripsinas, dipeptidades, carboxipeptidase e α-amilases. Cento e trinta e dois clusters (190 ESTs) codificavam para sequências de metabolismo em energético, síntese de proteínas, transdução de sinal e outras funções celulares. Noventa e cinco clusters (231 ESTs) codificaram para proteínas sem similaridades com proteínas conhecidas no banco de dados. Estudos de expressão, localização e imunocitoquímica de sequências alvos deverão no futuro nos fornecer um melhor entendimento da fisiologia digestiva da larva de Musca domestica em detalhes moleculares. Uma enzima lipolítica (LipMD) foi identificada no item anterior. A sequência de aminoácidos obtidas é homóloga a lipases e contém os três bem conservados resíduos de aminoácidos que compõe a tríade catalítica (Ser183, His273 e Asp208) para esse grupo de enzimas. O fragmento de cDNA codificante para a LipMD foi clonado em vetor pAE (Ramos et al., 2004) e expresso em E. coli produzindo uma enzima com massa molecular de 37,3 kDa. A LipMD recombinante foi purificada e é capaz de hidrolizar tributirina e um grande número de substratos (pNP-acetato a pNP-esterato) e possui um pH ótimo próximo de 7,5. Analise por RT-PCR mostrou que os transcritos codificantes para a LipMD são expressos apenas no ventrículo anterior. Western-blots após SDS-PAGE das proteínas de vários tecidos e marcados com o anticorpo produzido contra a LipMD revelou a ocorrência da enzima principalmente no conteúdo luminal do ventrículo anterior. A LipMD é localizada pela técnica de imuno-ouro no interior de vesículas de secreção próximas a microvilosidades no interior das células do ventrículo anterior. Esta enzima pode atuar como uma enzima lipolítica digestiva secretada nessa região do ventrículo de larvas de M. domestica / The house fly, Musca domestica is one the best known and most widely distributed insects known to humans. M. domestica larvae display in anterior and middle midgut contents, a proteolytic activity with pH optimum of 3.0-3.5 and kinetical properties like cathepsin-D. Three cDNAs coding for preprocatepsin D-like proteinases (ppCAD1, ppCAD2, ppCAD3) were cloned from a M. domestica midgut cDNA library. The sequences encoding the signal peptide, propeptide and mature enzyme having all conserved catalytic and substrate binding residues found in bovine lysosomal cathepsin-D. A cladogram of sequences of insect and vertebrate cathepsin-D-like proteinases deposited on GENBANK form a large grouping divided into two monophyletic branches: one with vertebrate and the other with insect lysosomal sequences including ppCAD1. Human pepsinogen, ppCAD2, ppCAD3, and a sequence from Drosophila melanogaster are excluded indicating a nonlysosomal function for them. CAD3 should correspond to the digestive CAD found in enzyme assays because: (1) The mRNA for CAD3 is expressed (RT-PCR) only in the anterior and proximal middle midgut. (2) Recombinant pCAD3, after auto activation has a pH optimum of 2.5-3.0 that is close to the luminal pH of M. domestica midgut. (3) Immunoblots of proteins from different tissues and stained with antiserum prepared against recombinant pCAD3 were positive only for the anterior and middle midgut tissue and contents. (4) CAD3 is localized with immunogold labeling inside secretory vesicles and around microvilli in anterior and middle midgut cells. The data support the view that on adapting to a detritivorous habit M. domestica digestive CAD (and of other Diptera Cyclorrhapha) resulted from the same archetypical gene as the intracellular cathepsin-D, paralleling what happened with vertebrates. A limited amount of data regarding DNA sequences and molecular aspects of Musca domestica species is avaliable. We proposed to generate ESTs sequences from a cDNA library constructed from larval midguts. A total of 826 randomly selected midgut derived cDNAs were sequenced and assembled based on their similarities into 323 clusters. The sequences were classified into three categories: (S) probably secretory products, (H) housekeeping products and (U) products with unknown cell localization and function. One hundred and sixty clusters (423 ESTs) encode putative secreted proteins such as lysozymes, lipases, trypsins, chymotrypsins, dipeptidases, carboxypeptidases A and α-amylases. One hundred and thirty two clusters (190 ESTs) encode housekeeping sequences associated with energy metabolism, protein syntesis, signal transduction and other cellular functions. Ninety five clusters (213 ESTs) encode proteins with no similarity with known proteins. Expression and high-throughput bioassay screening of target sequences must provide us with a better understanding of the digestive physiology of Musca domestica midgut larvae, in molecular detail. A lipolytic enzyme (LipMD) was identified from expressed sequence tags (EST) constructed from midgut larvae cDNA library. The deduced amino acid sequence is homologous to lipases and contained the three well-conserved amino acid residues, Ser183, His273, and Asp208, which form the catalytic triad of the enzyme. The cDNA fragment encoding for LipMD was cloned into a pAE vector (Ramos et al., 2004) and expressed in E. coli producing an enzyme with a molecular mass of 37.3 kDa. The recombinant LipMD was purified and was able to hydrolyse tributyrin and a broad range of substrates, from C2 to C18 p-nitrophenyl-esters and displayed an optimal pH of approximately 7.5. RT-PCR analysis in tissue homogenates (anterior, middle and posterior midguts, hemolymph, fat body and Malpighian tubules) showed that LipMD mRNA transcripts were expressed only in anterior midgut. Western-blots after SDS PAGE of proteins from different tissues and stained with anti-LipMD serum revealed that the enzyme occurs mainly in the anterior midgut lumen. LipMD is localized with immunogold labeling inside secretory vesicles and around microvilli in anterior midgut cells. LipMD is a candidate to be the digestive lipolytic enzyme found in that midgut region
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Catepsinas B vitelolíticas de Culex quinquefasciatus. / Viteolytic cathepsinas B of Culex quinquefasciatus.

Alexandre Santos de Moura 21 February 2014 (has links)
Apesar de Culex quinquefasciatus ser um eficiente vetor de doenças tais como a filariose linfática, febre do Nilo Ocidental e outras várias neuroviroses, poucas pesquisas sobre sua fisiologia têm sido conduzidas. Como em todos os animais ovíparos, o desenvolvimento embrionário dos mosquitos depende da degradação dos nutrientes armazenados no ovo, sendo a catepsina B uma protease que tem sido identificada e caracterizada em vários insetos como envolvida nesta função. Neste trabalho identificamos, por espectrometria de massas, duas catepsinas B de Culex quinquefasciatus, parcialmente purificadas por autoproteólise de extrato total de ovos. Segundo a anotação de suas sequências no banco de dados específico para vetores, o VectorBase, elas são enzimas parálogas e suas sequências apresentam 77% de homologia. Denominadas neste trabalho como CatB1 e CatB2, ambas são expressas simultaneamente no corpo gorduroso de todas as fêmeas vitelogênicas de nossa colônia e sua atividade pode ser detectada nos ovários vitelogênicos, sugerindo sua origem materna. A transcrição de ambas as enzimas se inicia após o repasto sanguíneo (ARS), alcançando o pico de expressão às 36 h ARS, de forma bastante semelhante à da vitelogenina. / Despite Culex quinquefasciatus be an efficient vector of diseases such as lymphatic filariasis, West Nile fever and other various neurotrophic viruses, little research on its physiology have been conducted. As in all oviparous animals, embryonic development of mosquitoes depends on the degradation of the nutrients stored in the egg. Cathepsin B is a protease that has been identified and characterized in a number of insects as involved in this function. In this work we have identified, by mass spectrometry, two cathepsins B of Culex quinquefasciatus, partially purified by self-proteolysis of total egg extract. According to the annotation of their sequences in the specific vector database, the VectorBase, they are paralogue enzymes and their sequences have 77% homology. Named in this work as CatB1 and CatB2, both are expressed simultaneously in the fat body of all vitellogenic females of our colony and its activity can be detected in vitellogenic ovaries, suggesting a maternal origin. The transcription of both enzymes starts post blood meal (PBM), reaching their peak of expression at 36 h PBM, quite similar to vitellogenin.
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Significations physiopathologiques des hémorphines de type 7 dans le diabète et les cancers broncho-pulmonaires / Physiopathological significations of hemorphin-7 in diabetes and lung cancers

Féron, Delphine 30 April 2010 (has links)
Les hémorphines représentent une classe de peptides cryptiques bioactifs issus de la protéolyse de la chaîne ß de l’hémoglobine dont la présence in vivo a souvent été associée à des conditions physiologiques ou pathologiques particulières, comme les cancers. De ce fait, l’hypothèse d’utiliser ces peptides comme marqueur de pathologies avaient déjà été posé. Des études récentes au laboratoire ont montré que leur concentration sérique des hémorphines était diminuée chez les patients diabétiques. Nous avons travaillé sur une cohorte de 120 patients atteints de diabète de type 1 et 2 et nous avons étudié les différentes hypothèses permettant d’expliquer cette diminution : métabolisme des hémorphines, impact de la glycation de l’hémoglobine sur la libération des peptides. De plus, nous avons cherché à connaître le rôle de LVVH7 dans la signalisation insulinique. Les résultats obtenus suggèrent que la diminution de la concentration sérique des hémorphines de type 7 est spécifique du diabète mais que la glycation de l’hémoglobine n’a pas d’impact sur la libération des hémorphines. Parallèlement à cette étude, nous avons caractérisé les hémorphines de type 7 dans le microenvironnement du cancer broncho-pulmonaire. La cathepsine D, enzyme impliquée dans la libération de LVVH7 et VVH7 ainsi que dans le microenvironnement tumoral, a été étudié dans des cultures de lignées de cancers broncho-pulmonaires acidifiée et donc propice à la progression tumorale. Les résultats ont permis de confirmer la présence de la cathepsine D dans le surnageant de cultures des cellules cancéreuses. De plus, nous avons montré la libération d’hémorphines de type 7 dans des milieux de culture acidifiés. / Hemorphins are cryptic bioactive peptides derived from ß chain of haemoglobin proteolysis. Their biological presence has often been associated with physiological or pathological conditions, like cancers. Thus, it was already suggested that hemorphins could be used as marker of pathology. Previous studies achieved in our laboratory demonstrated that hemorphin-7 was decreased in serum of diabetic patients. We worked on a population of 120 patients with type 1 and type 2 diabetes and we studied different hypothesis: hemorphins metabolism, impact of glycated haemoglobin. Moreover, we researched the role of LVVH7 in insulin signalisation. The results suggested that hemorphin-7 concentrations in serum of diabetic patients are specific of this pathology but glycation of haemoglobin have no impact on liberation of hemorphins. In parallel, we characterized hemorphin-7 in tumor progression of lung cancers. Cathepsin D, previously demonstrated as a key enzyme in hemorphins generation and also in tumor progression, has been studied in vitro in lung cancers, cultured in acidified conditions, favourable to metastasis. In addition, we also identified hemorphin-7 liberation in acidified media culture.
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Estrutura e função das cisteína proteinases intestinais do besouro Tenebrio molitor / Structure and function of intestinais cysteine proteinases of Tenebrio molitor beetle

Daniela Beton 17 December 2009 (has links)
A catepsina L é uma cisteína proteinase da família da papaína (clã CA, família C1), sendo esta família a mais conhecida entre as cisteína proteinase. A catepsina L, como outras proteinases da família C1, é sintetizada como uma pró-enzima inativa que é ativada através da remoção do pró-peptídeo. Os pró-peptídeos das catepsinas da subfamília catepsina L apresentam um motivo consenso, denominado motivo ERFNIN. A catepsina L corresponde a principal proteinase digestiva em Tenebrio molitor. No nosso laboratório 3 pró-catepsinas L (pCALs) foram clonadas e seqüenciadas a partir de uma biblioteca de cDNA de intestino médio de larvas de T. molitor: pCAL1 (CAL lisossomal), pCAL2 e pCAL3 (enzimas digestivas). Estas proteinases apresentam o motivo ERFNIN e os resíduos envolvidos na catálise: Cys25, His169, e Asn175 com Gln19 (numeração da papaína). Neste trabalho descrevemos a clonagem em vetores de expressão e a expressão em bactérias das sequências codificadoras de pCAL1, pCAL2 e pCAL3. As pró-catepsinas L recombinantes foram purificadas por cromatografia de afinidade e a incubação em pH ácido resultou na formação das enzimas maduras CAL1, CAL2 e CAL3 com atividade sobre o substrato Z-FR-MCA. O anticorpo policlonal anti-pCAL2 foi produzido em coelho e reconheceu pCAL2 e CAL2 em immunoblots. Experimentos de immunoblots com diferentes tecidos de T. molitor mostraram que o anticorpo policlonal anti-pCAL3 reconheceu pCAL3 e CAL3 nos dois terços anteriores do intestino médio de larvas de T. molitor. Estudos de imunocitolocalização indicam que a catepsina L 3 ocorre em vesículas no intestino médio anterior e em microvilosidades no intestino médio posterior. Para os experimentos de cristalização, nós expressamos pCAL1, pCAL2 e pCAL3 como mutantes Cys25→Ser inativos. pCAL3Cys26Ser foi cristalizada por difusão de vapor (gota sentada) contra 0,1-1,6M de dihidrogênio fosfato de amônio. Os cristais são monoclínicos com grupo espacial C2 e parâmetros de célula: a=57,634 Å, b=89,322 Å, c=70,076 Å, α=γ=90°, β=92,502° e uma molécula na unidade assimétrica. A e strutura foi determinada por substituição molecular usando a estrutura de Fasciola hepatica (42% de identidade) como modelo. O modelo foi refinado a 2,1 Å com fator R final de 16,19% (Rfree=20,5%). pCAL2Cys25Ser foi cristalizada por difusão de vapor (gota sentada) contra acetato de sódio 0,2M, cacodilato de sódio 0,1M pH6,6-6,7 e 20% de PEG 8000. Os cristais são triclínicos com grupo espacial P1 e parâmetros de célula: a=51,669 Å, b=52,37 Å, c=59,716 Å, α= 91,278°, γ=109,586°, β=91,547° e duas moléculas na unidade assimétrica. A estrutura foi determinada por substituição molecular usando a estrutura da pCAL3 (44% de identidade) como modelo. O modelo foi refinado a 2,0 Å com fator R final de 17,61% (Rfree=22,48%). A estrutura terciária da pró-catepsinas L digestivas é muito similar as estruturas de cisteína proteinases da família da papaína / Cathepsin L is a cysteine proteinase of the papain family (clan CA, family C1), which is the most known among the cysteine proteinases. Cathepsin L, like other proteinases of family C1, is synthesized as an inactive proenzyme that is activated by propeptide removal. The propeptide of cathepsin L-like subfamily contain a highly conserved motif, the so called ERFNIN motif. Cathepsin L corresponds to the major digestive proteinase in Tenebrio molitor. In our laboratory, 3 procathepsins L (pCALs) were cloned and sequenced from a cDNA library prepared from T. molitor larval midguts: pCAL1 (lysosomal CAL), pCAL2 and pCAL3 (digestive enzymes). These proteinases have ERFNIN motif and 3 residues directly involved in catalysis: Cys25, His169, Asn175 with Gln19 (papain numbering). In this work we report the cloning into the expression vector and bacterial expression of the sequences coding pCAL1, pCAL2 and pCAL3. The recombinant procathepsins L were purified by affinity chromatography and activation of these enzymes occurs under acidic conditions. The cathepsins L (CAL1, CAL2 and CAL3) were able to hydrolyse Z-FR-MCA. The polyclonal antibody anti-pCAL2 was produced in rabbit and recognized pCAL2 and CAL2 on immunoblots. Immunoblot analyses of different T. molitor larval tissues demonstrated that the polyclonal antibody anti-pCAL3 recognised pCAL3 and CAL3 in the anterior two-thirds of midgut tissue of T. molitor larvae. Immunolocalization studies indicate that cathepsin L 3 occurs in vesicles in the anterior midgut and microvilli in posterior midgut. To crystallographic studies we expressed pCAL1, pCAL2 and pCAL3 as inactive Cys25→Ser mutants. pCAL3Cys26Ser was crystallized by vapor diffusion in sitting drops against 0.1-1.6 M mono-ammonium dihydrogen phosphate. The crystals are monoclinic, belonging to space group C2, with cell parameters: a = 57.634 Å, b = 89.322 Å, c = 70.076 Å, α = γ =90°, β = 92.502° and contain one molecule in the asymmetric unit. The structure was determined by molecular replacement using the structure of Fasciola hepatica procathepsin L (42.5% identity) as a model. The model was refined at 2.1 Å resolution with an R factor of 16.19% (Rfree = 20.5%). pCAL2Cys25Ser was crystallized by vapor diffusion in sitting drops against 0.2M sodium acetate, 0.1M sodium cacodylate pH 6.6-6.7 and 20% polyethylene glycol 8,000. The crystals are triclinic, belonging to space group P1, with cell parameters: a = 51.669 Å, b = 52.37 Å, c = 59.716 Å, α = 91.278° γ = 109.586°, β = 91.547° and contain two molecules in the asymmetric unit. The structure was determined by molecular replacement using the structure of procathepsin L 3 (44 % identity) as a model. The model was refined at 2.0 Å resolution with an R factor of 17.61% (Rfree = 22.48%). The tertiary structure ofdigestive procathepsins L is very similar to papain-like cysteine proteinases structures

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