The aim of this master thesis is to focus on a various methods of the conjugation of palladium nanoparticles of different shapes on the protein. The main point was to use both covalent and non-covalent conjugation of palladium nanoparticles on the protein and to use the functional conjugates to find out their stability in time.

Orientador: Paulo Regis Caron Ruffino / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Matematica, Estatistica e Computação Cientifica / Made available in DSpace on 2018-08-12T16:41:27Z (GMT). No. of bitstreams: 1 Silva_FabianoBorgesda_D.pdf: 848923 bytes, checksum: 27f2cf2ad665ac271db23db385dab86f (MD5) Previous issue date: 2009 / Resumo: Este trabalho consiste basicamente em três níveis de decomposições de fluxos estocásticos: 1) decomposição via G-estruturas; 2) decomposição com componente em trajetórias hamiltonianas e 3) conjugações de fluxos aleatórios ¿Observação: O resumo, na íntegra poderá ser visualizado no texto completo da tese digital. / Abstract: This thesis concerns three different kind of decomposition of stochastic flows: 1) decompositions preserving G-structures; 2) decompositions with a component whose trajectories are hamiltonians and; 3) tensor preserving conjugacies with random time differentiable cociclos ...Note: The complete abstract is available with the full electronic digital thesis or dissertations. / Doutorado / Sistemas Dinamicos / Doutor em Matemática

Kyoto University (京都大学) / 0048 / 新制・課程博士 / 博士(工学) / 甲第15364号 / 工博第3243号 / 新制||工||1488(附属図書館) / 27842 / 京都大学大学院工学研究科都市環境工学専攻 / (主査)教授 田中 宏明, 教授 藤井 滋穂, 教授 米田 稔 / 学位規則第4条第1項該当

Pendant longtemps, l’erreur était considérée comme l'effet de l'ignorance. Etant donné l’omniprésence de l’erreur dans l’apprentissage, elle occupe une place importante au centre de la didactique moderne. Cette thèse vise à étudier le rôle de l’erreur en production écrite dans l’apprentissage du français chez les étudiants persanophones qui apprennent le français comme langue étrangère. Elle se matérialise autour de la conjugaison du verbe et l’emploi des formes temporelles. Il s’agit d’une expérimentation didactique dans laquelle des pédagogies différentes apparaissent traitant l’erreur chacune d’une manière différente. Le résultat obtenu montre que les erreurs sont moins persistantes dans une méthode basée sur le modèle socioconstructivisme où l'enseignant les prend en compte de façon spécifique, y accordant un statut qui ne dévalorise pas l’auteur, alors que les mêmes résultats dans les méthodes traditionnelles montrent que la réitération de l’enseignement des règles ne suffit pas à résoudre des difficultés qui relèvent davantage du maniement que du savoir. Nous nous sommes proposée de modifier de perspective et de partir des productions des étudiants et de leurs stratégies et faire participer l’étudiant dans une activité réflexive. Le résultat de cette expérimentation nous a permis de corroborer l’hypothèse de cette recherche, selon laquelle les étudiants soumis à une pédagogie de la découverte feront moins d'erreurs que ceux qui sont soumis à une pédagogie traditionnelle. / In a long period of time, we discovered that error was caused by ignorance. Although errors are always in learning, they do big play role and locate in an important aspect of modern didactic. The purpose of this research for educational students in learning French foreign language is to study the role of error at writing. In this instructional method, we worked on conjugation and the use of different forms of the tense. The main subject is this: there is an experimental didactic instruction for us which encounter impressive action toward error. The recent consequences demonstrated that in some method which coded in terms of social-constructivism model, the error can be disappeared easily rather than other models. That means the persistence of the errors were considerably low. In this method, teacher treats significantly with error, she confers it a particular status, in this way student fells less downgrading. In compeer to traditional methods which focuses on teaching repetitive grammar, as a result it cannot entirely guarantee student’s weaknesses and help them enhance, because the problem was not related to knowledge, it related to handling. We proposed to modify our perspective and study student’s writing, in addition their strategy and finally force them to participate in some mental activity. These results of research let us certify these hypothesis according to our experiment, those students who participated in this new pedagogy caused less errors rather than those students in traditional pedagogy.

>Magister Scientiae - MSc / The therapeutic goal of cancer treatment is to trigger selective cell death in cancer cells. To eliminate cancerous cells effectively, the anti–cancer drugs must be targeted to the affected cells. However, anti–cancer drugs are often distributed non–specifically giving rise to systemic toxicities and other adverse effects. Cancer specific peptides are useful cancer targeting agents that can be used for the targeted delivery of anti-cancer drugs. Several cancer targeting peptides and some of their corresponding protein targets have been identified. Previous work investigated the specific binding of five of these peptides (p.C, p.H, p6.1, Frop-1 and p.L) conjugated to fluorescent nanoparticles (quantum dots) to a panel of human cell lines, which included four cancerous cell lines (Caco-2, HeLa, HT29 and HepG2) and one non-cancerous cell line (KMST-6). Flow cytometry showed that the p.L peptide preferentially bind to HT29 cells; suggesting that the expression levels of the target for the p.L peptide are higher in these cells. The objective of this study was to make use of target specific functionalised quantum dots (QDs) to deliver Second mitochondria-derived activator of caspases/ Direct AIP binding protein with low PI (Smac/DIABLO) to HT29 cells with the aim of enhancing the effects of pro-apoptotic drugs. Smac/DIABLO is a pro-apoptotic peptide that is able to interact with inhibitor of apoptosis proteins (IAPs), thereby inducing pro-apoptotic signalling. Methodology: CdSe/ZnS core-shell QDs were synthesised using the one-pot synthesis method. These QDs were characterised using photoluminescence (PL) spectroscopy, high resolution transmission electron microscopy (HR-TEM) and energy dispersive x-ray spectroscopy (EDS). The CdSe/ZnS core-shell QDs were solubilised with L-cysteine (Cys- QDs). The Cys-QDs were bi-conjugated to the p.L peptide and Smac peptide using 1-ethyl-3- (30-dimethylamino) carbodiimide (EDC) chemistry. Cultured HT29 cells were exposed to the 10 | P a g e QD peptide bi-conjugates and fluorescence microscopy was employed to assess targeting and internalisation. The cytotoxicity of the QD peptide bi-conjugates in combinatorial treatment with ceramide was evaluated using the WST-1 Cell Proliferation assay. A commercially available QD with similar chemistry was used to carry out a comparative study to relate the efficiency of the in-house synthesized QD.

Over the past decade, solid-state nanopores have emerged as a versatile tool for the detection and characterization of single molecules, showing great promise in the field of personalized medicine as diagnostic and genotyping platforms. While solid-state nanopores offer increased durability and functionality over a wider range of experimental conditions compared to their biological counterparts, reliable fabrication of low-noise solid-state nanopores remains a challenge. In this thesis, a methodology for treating nanopores using high electric fields in an automated fashion by applying short (0.1-2 s) pulses of 6-10 V is presented which drastically improves the yield of nanopores that can be used for molecular recognition studies. In particular, this technique allows for sub-nanometer control over nanopore size under experimental conditions, facilitates complete wetting of nanopores, reduces noise by up to three orders of magnitude and rejuvenates used pores for further experimentation. This improvement in fabrication yield (over 90%) ultimately makes nanopore-based sensing more efficient, cost-effective and accessible. Tuning size using high electric fields facilitates nanopore fabrication and improves functionality for single-molecule experiments. Here, the use of nanopores for the detection of DNA-protein complexes is examined. As proof-of-concept, neutravidin bound to double-stranded DNA is used as a model complex. The creation of the DNA-neutravidin complex using polymerase chain reaction with biotinylated primers and subsequent purification and multiplex creation is discussed. Finally, an outlook for extending this scheme for the identification of proteins in a sample based on translocation signatures is presented which could be implemented in a portable lab-on-a-chip device for the rapid detection of disease biomarkers.

A synthetic approach to macromolecules of acetylenic arrays and luminescent properties is proposed and the execution of initial steps is described. Palladium-catalyzed coupling of 1,3,5-triiodobenzene with trimethylsilylbuta-1,3-diyne, trimethylsilylocta-1,3,5,7-tetrayne, and trimethylsilylhexadeca-1,3,5,7,9,11,13,15-octayne to yield the new 1,3,5-tris(trimethylsilylbuta-1,3-diynyl)benzene and the proposed 1,3,5-tris(8-(trimethylsilyl)octa-1,3,5,7-tetraynyl)benzene and 1,3,5-tris(trimethylsilyl)hexadeca-1,3,5,7,9,11,13,15-octaynyl)benzene respectively. The proposed three-coordinate Au (I) complexed macromolecules will be derived from the metallation of the aforementioned alkynylated arenes.

No description available.

This diploma thesis describes organic pigments of diketopyrrolopyrroles (DDPs) possessing properties applicable in attractive and perspective areas of organic electronics and photonics. The modification of the DPP skeleton was performed by nucleophilic substitution by various alkyl chains and 5 series of DPP derivatives were prepared. The regioselectivity of N-alkylation and also the photophysical properties of the prepared derivatives were studied. A key product of this work is the N,N'-ethyladamantyl derivative of DPP, which exhibited ambipolar characteristic with excellent electron mobility of 0.2 cm2 V–1 s–1. Further, the -conjugation of the above-mentioned DPP derivative was extended by 1 and 2 thiophene units at positions 3,6 and the effect of this modification on optical properties of the resulting derivatives was investigated. A new modified N,N'-unsubstituted DPP derivative was also prepared. The last point of this thesis was the study of the incorporation of formyl functional groups into the skeleton of key N,N'-ethyladamantyl DPP derivative.

Gene transfer plays an important role in bacterial evolution. Especially in an under explored species like Rhodococcus, a type of bacteria found in the soil. Rhodococcus has several applications in the pharmaceutical industry and in the production of antibiotics. Rhodococcus possess several unique sets of properties which makes it beneficial to have a reliable method of producing mutants of Rhodococcus. The goal of the experiment was to find an efficient way of forming Rhodococcus colonies with kanamycin resistant genes. The project began from an unexpected observation from an earlier experiment with Rhodococcus strain MTM3W5.2. where I attempted to transform this strain with a transposon via electro-transformation. The colonies that grew/ appeared transformants were screened to confirm the presence of kanamycin gene, however there was no amplified DNA seen on the PCR gel (i.e. absence of the kanamycin gene). The electro-transformant colonies were selected on LB plates containing different higher concentrations of kanamycin. Then the appeared transformants were again screened via disk diffusion assay and were classified into 3 different kanamycin resistant phenotypes. Majority of the “C” phenotypic colonies (i.e., high level resistance to kanamycin) appear to contain the kanamycin gene, but these colonies were less in numbers. This led us to try another method of gene transfer which is conjugation. Conjugation was carried on a double selection antibiotic plate containing both chloramphenicol (30 µg) and kanamycin (100 µg). The transconjugate colonies that appeared on the double selection plates were also screened by PCR, but none of the colonies had amplified DNA suggesting absence of the kanamycin gene. The colonies seen on the double selection plate were possibly due to spontaneous mutation or some type of unknown phenotypic variation. However, in the future, double selection plates with higher concentrations of antibiotics can possibly give us transconjugants with kanamycin genes.