Background: Phytoestrogens are diphenolic compounds found in plants with a structure and molecular weight similar to oestradiol which enables them to bind to the oestrogen receptor. Isoflavonoids occur mainly within the legume family with highest concentration in soybeans. Lignans are found in a range of plant foods and the richest known source is linseed. Few studies have been published on intake of isoflavonoids and none were located on intake of lignans in Australian women. The validity of methods designed to estimate intake can be assessed using urinary excretion of isoflavonoids and lignans as studies have found an association between intake and excretion of isoflavonoids and lignans. It has been proposed that, through their ability to act like oestrogen, phytoestrogens could decrease bone turnover and attenuate the loss of bone mineral density (BMD) at menopause. The aims of this research were to determine the pattern of intake of isoflavonoids and lignans in 500 women from food and supplements and to assess a questionnaire used to estimate intake using excretion in a sub-sample of 141 women. Associations between usual intake or excretion of isoflavonoids and lignans and biomarkers of bone health were also examined. Methods: A cross-sectional study was conducted involving 500 women aged 40-80 years participating in the Longitudinal Assessment of Ageing in Women (LAW), a 5 year study being conducted in the Betty Byrne Henderson Centre at the Royal Brisbane and Women's Hospital. Subjects were randomly selected from the electoral role and stratified into ten year age groups. Intake of isoflavonoids and lignans from food and supplements was assessed using a specially designed questionnaire containing 110 items. Values for individual items were obtained from published literature and summed to provide average daily intakes of isoflavonoids and lignans (mg/d). A sub-sample of 141 women was recruited to take part in the assessment of the association between phytoestrogen intake and excretion. Participants collected three 24-h urine samples spaced over one week. Samples were analysed using high performance liquid chromatography MS/MS for seven isoflavonoids and four lignans. Bone mineral densities (BMD) of the femur neck, total hip and lumbar spine were measured by dual energy x-ray absorptiometry. Bone formation was assessed using serum bone alkaline phosphatase (bone ALP) and osteocalcin (OC) and bone resorption was assessed using deoxypyridinoline (DPD) and urinary excretion of N-terminal cross-linking telopeptide of type-I collagen (NTX). Potential confounding factors were also evaluated. Statistical analyses were conducted using SPSS for windows (version 10). Participants were defined as consumers if they reported intake of one or more serves of soy or linseed in the prior month. Differences in socio-demographic and lifestyle characteristics between groups were assessed using ANOVA and Chi Square tests. Associations between intake and excretion of phytoestrogens were assessed using Spearman's rank-order correlations () for non-normal data. Phytoestrogen intake was categorised into four groups for the assessment of the association with markers of bone health. Associations between phytoestrogen excretion and markers of bone health were assessed using Pearson's product moment correlations for normal data (r) and Spearman's rank-order correlations for non-normal data. A value of P < 0.05 was taken as statistically significant. Results: Consumption of soy food was reported by 40% and consumption of linseed by 34% of women. Median (range) intakes among soy/linseed consumers for isoflavonoids, 3.87 (0-173) mg/d, and lignans, 2.40 (0.1-33) mg/d, were significantly higher than corresponding intakes among non consumers of 0.005 (0-2.6) and 1.57 (0.4-4.7) mg/d, respectively (P < 0.001). Soy/linseed consumers reported higher intakes of energy (P=0.043), dietary fibre (P=0.003) and polyunsaturated fat (P=0.004); and a higher level of physical activity (P=0.006), SEP (P < 0.001), education (P < 0.001) and supplement use (P < 0.001). Use of non-prescription supplements for menopause in the previous month was reported by 13% of women. A review of supplements available for treatment of menopause indicated that use of soy, red clover, black cohosh and sage could have a role in treatment of menopause symptoms. Evidence supporting the presence of oestrogenic components was available for soy and red clover isoflavonoids only. There was a significant association between intake and excretion of isoflavonoids within the total group (r=0.207, P < 0.05), with a stronger association in soy consumers (r=0.364, P < 0.01). Excretion of isoflavonoids was detected in women who did not report known intake of soy foods, suggesting isoflavonoids could be derived in small amounts from other plant foods or use of soy as an ingredient in processed foods. There was no significant association between intake and excretion of lignans, however both intake and excretion were associated with dietary fibre (r=0.303 and r=0.230, respectively, P < 0.01 for both). Bone ALP was higher among the very low isoflavonoid intake group (P=0.005) for the total sample (P=0.005) and women with BMI≤25 kg/m2 (P=0.002). Data also demonstrated an inverse association between excretion of isoflavonoids and NTX within women with BMI≤25 kg/m2 (r=-0.33, P < 0.05). There was a positive association between lignan excretion and bone ALP in the total sample (r=0.21, P < 0.05) which was strengthened in women with osteoporosis/osteopenia (r=0.41, P < 0.05) and a positive association between lignan excretion and DPD among women with BMI≤25 kg/m2 (ρ=0.28, P < 0.05) All associations remained significant after adjustment for confounding. Conclusions: Few women who chose phytoestrogen-rich foods consumed amounts similar to women with traditional soy-based diets although some achieved high intakes with supplements. Women who consumed soy or linseed foods differed in lifestyle and sociodemographic characteristics that could influence the association with disease in epidemiological studies. Results indicated that the phytoestrogen questionnaire was useful for assessment of isoflavonoids but was not acceptably precise for measurement of lignans. Findings suggest that there is an inverse association between isoflavonoid status and bone ALP and NTX although the precise mechanism of action has not been clarified. The association between lignan intake and bone is less well understood; however findings of a positive association with bone ALP indicate that further research on the lignan content of foods and the inclusion of lignans in studies is warranted.

In this experiment, the effects of (1) lipid type (soy bean oil, tallow and fish oil), (2) the period the fish oil was provided and (3) a dietary supplement containing conjugated linoleic acid (CLA), selenium (Se), vitamin E and vitamin C on pig performance and pork quality were studied. Forty-eight female pigs (PIC hybrids, with a mean live weight of 16.19 kg ± 1.56 SD) were obtained from a single commercial operation in the North Island of New Zealand. The pigs were rank ordered by weight and assigned to one of six dietary treatment groups. The diet base was either a combination of animal and plant feedstuffs (AT and PTS), plant feedstuffs only (PO, POS) or plant feedstuffs combined with fish oil (PFSe and PFSl). The diets also differed depending on the presence or absence of the nutritional supplement SanoviteTM and a vitamin C supplement. SanoviteTM is a trademarked dietary supplement containing CLA (BASF, Auckland, New Zealand), organic Selenium (Alltech Inc., Nicholasville, KY) and vitamin E (Morel et al., 2008). Diets POS, PTS, PFSe, PFSl contained SanoviteTM and a vitamin C supplement. Diets PO and POS were used to establish the effect of the supplementation with SanoviteTM and the vitamin C supplement. Pigs fed diet PFSe received plant feedstuffs and fish oil with supplement between days 1 and 35 and then diet POS up to day 84. Pigs fed diet PFSl received diet POS between days 1 and 35; plant feedstuffs and fish oil with supplement between days 36 and 56 and then diet POS up to day 84. Pigs in group PFSe and PFSl both received the same total amount of fish oil per pig (2.52 l/ 2.31 kg). Between days 1 and 56 of the experiment grower diets were fed, and finisher diets were fed between days 57 and 84 of the experiment. The pigs were kept in pens of six, but fed individually twice daily (at approximately 8 am and 3.30 pm) according to a fixed feeding schedule. Water was available at all times. Individual feed intakes were measured daily and live weight recorded weekly. Faeces were collected once a day during two days in week five of the trail for digestibility determination. Carcass quality characteristics determined at the abattoir included carcass weight and back fat thickness as measured at the end of the slaughter line. Meat quality assessments were performed on the Semimembranosus muscle (SM) from one of the topside cuts of each pig. Measurements of fatty acid profile (loin and backfat) and the Se content (lean meat) were conducted in Singapore by Mrs. J. Leong (MSc). In this study plant or animal feedstuffs, lipid type, SanoviteTM and vitamin C supplementation had no significant effects on growth performance and carcass quality. There were no differences in apparent faecal digestibility characteristics for dry matter (DDM) and organic matter (DOM) in the un-supplemented animal (AT) and plant based (PO) diets. Lipid type had a significant effect on the digestibility of ash (DA), and an increased ratio of unsaturated fatty acid to saturated fatty acid resulted in increases in DDM and DOM. DDM and DOM increased when soybean and linseed oil (POS) were used instead of tallow (PTS) or fish oil (PFS). The main differences in DDM, DOM and DA were observed between diets PO and POS. A positive effect of selenium, vitamin E and CLA supplementation is suggested. Increased cooking temperatures reduced tenderness (higher mean, peak force, yield force and peak force – yield force) and increased cooking loss. There was a significant negative relationship between ultimate pH and relative lightness (L*). There were highly significant positive correlations between all three measurements of expressed juice, and there was a significant positive correlation between cooking loss at 60 and 70oC (P<0.01) but correlations between expressed juice values and cooking loss were not significant. The P-values for the contrasts for cooking loss at 70 0C were significant for all contrasts except for AT vs PO and PFSe vs PFSl. For all other contrasts, the P-values for cooking loss at 70 0C were significantly higher in group POS than for groups PO, PTS and PFSe+PFSl. The P-value for (Peak force – Yield force) at 70°C was significantly higher in samples from group PFSe than for samples from group PFSl. The P-value for the myofibrillar fragmentation index (MFI) was significantly higher for group AT in contrast to group PO. Group POS had a significantly higher P-value for MFI in contrast to groups PO, PTS and PFSe and PFSl. Group POS had a lower P-value for sarcomere length in contrast to groups PFSe and PFSl. Group PFSe had a significantly higher P-value for sarcomere length in contrast to group PFSl. Group PFSe had a significantly higher P-value for expressed juice percentage loss in weight in contrast to group PFSl. Supplementing with SanoviteTM increased the Se content (P = 0.002) in lean meat as analysed by J. Leong (2010, personal communication). In general it was concluded that an increase in the ratio of unsaturated fatty acid to saturated fatty acid (U/S) in the diet resulted in higher levels of unsaturated fatty acids in loin and backfat. The fatty acid profile in the diet reflected the fatty acid profile of pork. Backfat of pigs fed diets including soybean and linseed oil contained higher levels of linoleic and α-linolenic acids. Diets PO and POS were used to establish the effect of the supplementation of CLA. The backfat of pigs fed diet POS contained higher levels of CLA (C18:2-trans-10, cis-12) and α-linolenic acid than pigs fed diet PO. The loin of pigs fed diet POS contained higher levels of palmitoleic and linoleic acid and CLA (C18:2-cis-9, trans-11) and lower levels of oleic acid than pigs fed diet PO. The use of fish-oil as a lipid type resulted in the highest levels of eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) in loin and backfat. The loin and backfat of pigs fed fish-oil in the second part of the grower phase (PFSl) contained higher levels of EPA, DPA and DHA than pigs fed fish-oil in the first part of the grower phase (PFSe). By enriching the swine diet with long-chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA) it was possible to increase the EPA, DPA and DHA content of pork. Enriching pork with LC n-3 PUFA will contribute to achieving standards for adequate intake (AI), but might not be suitable to reach suggested dietary targets (SDT). In conclusion, it was possible to change the pork composition by dietary manipulation without compromising pig performance and meat quality. There were a few significant effects from treatments on meat quality characteristics, but differences reported in this study were small and relatively unimportant. A negative influence of the dietary regime on palatability and meat processing was expected, but these issues are beyond the scope of this experiment.

Thesis (M.S.)--University of North Carolina at Wilmington, 2005. / Includes bibliographical references (leaves: 90-97)

Thesis (MNutr (Interdisciplinary Health Sciences. Human Nutrition))--University of Stellenbosch, 2009. / ENGLISH ABSTRACT: The aim of the study was to investigate current perceived role of nutritional supplements in adolescent (16-18 years) male rugby players and establish usage practices within Kwazulu Natal (KZN) schools. Methods The nutritional supplementation practices of 68 rugby players from 7 KZN secondary schools were surveyed using an anonymous paper-based questionnaire. The boys were asked to identify from a list (with Other as a selection) which supplement they used, the frequency of use, sources of supplement information and advice they base their choices on, where products were bought from, reasons for use, average monthly spend on buying these supplements, whether dietary changes were made in conjunction with taking a supplement and what was their understanding of the role of supplementation in achieving their performance goals. Results Sixty eight out of 236 invited participants completed the questionnaire. This translated into a response rate of 29%. Fifty four percent of participants admitted to using nutritional supplements, protein and creatine being the most popular products listed (43% and 22% of supplement users, respectively). Thirty five percent of supplement users supplemented daily and 24% supplemented 3-4 times per week. Friends were the most popular source of advice and information regarding nutritional supplements 32% (n=12), with supplement company representatives the next most commonly used source 22% (n=8). Seventy percent (n=26) of supplements are bought from a pharmacy, with an average monthly cost of R250, but ranging from R30 to as much as R1500 per month. Seventy percent (n=26) indicated that they also made dietary changes in addition to taking the nutritional supplement. These dietary changes included making healthier food choices 81% (n=21), increasing intake of protein foods 65% (n=17), planned snacks around exercise 35% (n=9), increasing carbohydrate-rich foods 62% (n=16), increasing fruit and vegetable intake 50% (n=13), and including snacks between meals 35% (n=9). The study participants rated practice sessions and weight training as most important in terms of helping them achieve their goals; diet, rest and supplements were similarly ranked as being between fairly to very important. Twenty two percent admitted that they would consider taking an illegal supplement if it would assist them in achieving their goals. Conclusions This study indicates that at least half of rugby-playing school boys (age 16-18 yrs) are making use of some form of supplementation, with protein and creatine supplementation being the most popular. The data indicate that rugby-playing school boys see their peers as a good source of information, and are willing to spend a large amount of money obtaining it (about R250 per month on average). This is concerning as peer pressure combined with lack of knowledge on nutritional supplement usage (and nutrition) can lead to widespread misuse of supplements, and potential detrimental side-effects in this young study population. However it highlights the value that school-level educational programmes (age and sport specific) can have in improving supplement usage practices and creating sound nutritional practices amongst this population, better equipping them at making informed decisions. In addition, educational programmes should be extended to other influential sources of information such as school coaches, teachers and parents. / AFRIKAANSE OPSOMMING: Die doel van die studie was om die huidige persepsie oor die waarde van voedingsupplemente en die gebruikspraktyke daarvan in 16 -18 jarige adolessente manlike atlete in Kwazulu Natal (KZN) skole te bepaal. Uitkomste van die studie was om die voorkoms en tipe supplemente wat gebruik word, redes aangevoer vir die gebruik daarvan, kennis oor die rol van supplemente asook die bron van inligting te bepaal. Metodes Die voedingsupplementasie praktyke van 68 rugby spelers uit 7 KZN sekondêre skole is ondersoek deur gebruik te maak van n annonieme vraelys (papier basis). Die seuns is gevra om van n lys (die opsie ander was ingesluit) te identifiseer watter supplement hulle gebruik, die frekwensie van gebruik, die bronne van inligting en raadgewing ontvang, waar die produk aangekoop is, redes vir gebruik, gemiddelde maandelikse kostes aangegaan en of dieetveranderinge tesame met die supplementasie aangegaan is. Kennis rondom die rol van supplementasie in prestasie doelwitte is getoets. Resultate: Agt-en-sestig uit n totaal van 236 deelnemers wat uitgenooi is om deel te neem aan die studie, het die vraelys voltooi. Dus het 29% van die studie-deelnemers het dus op die vraelys gereageer. Vier en vyftig persent van die deelnemers het erken dat hulle supplemente gebruik waarvan kreatien en proteïen gelys is as die mees gewildste produkte (onderskeidelik 43% en 22%). Vyf en dertig persent het daagliks supplemente gebruik en 24% het 3-4 keer per week supplemente gebruik. Vriende was die mees gewildste bron van raad en inligting (32%), gevolg deur supplement maatskappy verteenwoordigers (22%). Sewentig persent van supplemente word gekoop by n apteek en n gemiddelde maandelikse bedrag van R250 word gespandeer, maar dit wissel van R30 tot soveel as R1 500 per maand. Sewentig persent het erken dat hulle dieet veranderinge in hul dieet tesame met die supplementasie aanbring. Hierdie veranderinge het die volgende ingesluit: die keuse van gesonder voedselsoorte (81%); n verhoogde inname van proteïenryke voedselsoorte (65%); beplanning van peuselhappies rondom oefening (65%); verhoogde inname van koolhidraatryke voedsel (62%); meer vrugte en groente (50%) en die neem van peuselhappies tussen maaltye (35%). Die deelnemers het oefening met gewigte en oefensessies as die mees belangrike faktore geag om hul doelwitte te bereik. Dieet, rus en supplemente is daarnaas gelyk geag as redelik belangrik en 22% het erken dat hulle n verbode middel sal gebruik indien dit hulle sal help om hulle doelwitte te bereik. Gevolgtrekkings Die studie wys dat ten minste die helfte van skoolseuns wat rugby speel (16-18 jr) een of ander vorm van supplementasie gebruik, waarvan proteïen en kreatien die mees gewildste is. Die data dui daarop dat skoolseuns wat rugby speel hul tydgenote ag as n goeie bron van inligting oor supplement gebruik en dat hulle bereid is om groot bedrae geld te spandeer om die supplemente te bekom (gemiddeld R250,00 per maand). Dit is kommerwekkend aangesien groepsdruk tesame met n gebrek aan kennis oor supplementasie (en voeding) kan lei tot algemene misbruik van supplemente en moontlike newe effekte in hierdie jong studie populasie. Dit beklemtoon egter ook die waarde wat skool gebasseerde opvoedingsprogramme kan hê om die bewustheid en kennis oor supplement gebruik in hierdie populasie te verbeter om hul in staat te stel om ingeligte besluite te neem. Dit moet ouderdom -en sportspesifieke voedingsonderrig insluit. Opvoedingsprogramme moet ook uitgebrei word na ander partye wat invloedryke bronne van inligting is soos skool afrigters, onderwysers en ouers.

CAPES / Dois experimentos foram conduzidos, com o objetivo de avaliar o efeito da suplementação, associada ou não, ao consórcio de leguminosas, no perfil de ácidos graxos da carne de novilhos terminados em pastagem de inverno. Os estudos foram conduzidos na Universidade Tecnológica Federal do Paraná - UTFPR, Campus Dois Vizinhos-PR. No primeiro experimento foram comparados os tratamentos: pastagem de aveia branca (Avena sativa L.) (AV), aveia + ervilhaca (Vica sativa) (AV+LEG) e aveia + suplementação energética, com base de milho triturado na proporção de 1% do peso vivo (AV+SUP). Nesse experimento, foram utilizados 18 novilhos machos, castrados, 1/4 Marchigiana, 1/4 Aberdeen Angus, 1/2 Nelore, com peso médio inicial de 360 kg e 19 meses de idade. Os animais apresentaram ao abate 2,39 mm de espessura de gordura e peso médio de 412,78 kg. Novilhos suplementados apresentaram maior teor de lipídios no músculo longissimus dorsi e gordura intramuscular mais saturada. Os animais terminados com aveia e suplementação energética apresentaram carnes com maior teor de ácidos graxos monoinsaturados e não houve efeito da dieta sobre o total de ácidos graxos poliinsaturados. Os animais que receberam ervilhaca na pastagem apresentaram carne com menor teor de ácido linoléico conjugado (CLA) que os demais. A utilização de pastagens nas dietas de ambos os tratamentos proporcionou relação n-6:n-3 mais benéfica e carne com gordura intramuscular mais benéfica. No segundo experimento, utilizaram-se 18 novilhos da raça Nelore, castrados, com vacina imunológica 30 dias antes do início do experimento, com peso de abate de 472,55 kg e 26 meses de idade. Sendo 8 animais alimentados com aveia + azevém + suplementação (AS); 8 animais alimentados com aveia + azevém + ervilhaca (AE) e 8 animais alimentados com aveia + azevém + ervilhaca + suplementação (AES). O período de pastejo foi de 100 dias. Os resultados apontam que a quantidade de ácidos graxos mirístico (C14:0), palmítico (C16:0) e esteárico (C18:0) não foram afetados (P>0,05) pelo tipo de dieta fornecida aos animais. Na análise de contraste, os animais alimentados com gramíneas apresentam maior teor do ácido graxo láurico (C12:0). Já os teores totais de ácido oleico representaram 80% e não se diferiram entre os tratamentos. Bovinos alimentados com AES apresentaram maiores (P<0,05) concentrações do ácido pentadecanóico. Os ácidos graxos poli-insaturados não se distinguiram nos tratamentos, com exceção do CLA que tiveram maiores teores na alimentação de AE. Houve variação de CLA para as dietas utilizando-se de leguminosas, com menores concentrações. Não houve efeito da dieta sobre o total de AGPI. Não houve variação entre os tratamentos para efeito da dieta na soma de n-6 e n-3, bem como, para relação n-6:n-3 na gordura intramuscular. O tratamento com milho apresentou maior relação n-6:n-3. No primeiro experimento com base de dieta em n:6:n-3, verificou-se que a carne ultrapassou o limite de 4 para a máxima relação n-6:n-3, o que a torna imprópria para o consumo humano. Já no segundo experimento, dispondo a leguminosa como principal alimento da dieta, verificou-se melhor qualidade para o consumo humano, para com a relação n-6:n-3, ainda que este tratamento demonstrou carne abaixo do limite de 4. / Two experiments were conducted with the objective of evaluate the effect of supplementation associated or not, the consortium of legumes in the fatty acid profile of beef steers finished in winter pasture acid profile. This study was conducted at the Federal Technological University of Paraná - UTFPR, Campus Dois Vizinhos-PR. In the first experiment compared the treatments: fed on pasture white oat (Avena sativa L) (AV); oat+vetch (Vica sativa) (AV+LEG) and oat+ supplementation energetic, with ground corn base in the proportion of 1% of body weight (AV+SUP). Were used, in the first experiment, 18 steers, male, castrated, 1/4 Marchigiana, 1/4 Aberdeen Angus, 1/2 Nelore, with average initial weight of 360 kg and 19 months old. The slaughter was established with an average of 2.39 mm of fat in animals. Steers present a greater lipid content in the longissimus dorsi muscle and more saturated intramuscular fat. The animals finished with oats and energy supplementation presented meats with higher content of monounsaturated fatty acids and there was no effect of diet on total polyunsaturated fatty acids. Animals fed only with oats, and oat with energy supplementation, presented meat more conjugated linoleic acid content (CLA). The termination on pastures afforded ratio omega-6/omega-3 most beneficial and meat more beneficial intramuscular fat. In the second experiment, were used 24 steers Nelore, castrated, with immune vaccine 30 days before starting the experiment, with slaughter weight of 472.55 kg and 26 months old. Being, 8 animals fed with pat + ryegrass + supplementation (AS); 8 animals fed with oat + ryegrass + vetch (AE); and 8 animals fed with oat + vetch + ryegrass + supplementation (AES). The experiment lasted 100 days. Results point out that levels of myristic fatty acids (C14:0), palmitic (C16:0), and stearic (C18:0) were not affected (P>0,05) by the diet type fed to the animals. In contrast analysis, the animals fed on grasses present a greater content of lauric fatty acid (C12:0). Already the total contents of oleic acid represent 80% and not differ between treatments. Steers fed AES had higher (P<0,05) pentadecanoic acid concentrations. Polyunsaturated fatty acids are not distinguished in the treatments, with the exception of CLA, which had higher contents in AE supply. There was variation in CLA diets for using legume, at lower concentrations. There was no effect of diet on the total AGPI. There was no variation between the treatments effect of diet on the sum of n-6 and n-3 and, compared to n-6:n-3 on intramuscular fat. Treatment with corn has a higher relation n-6:n-3. In the first experiment based diet n-6:n-3 it appeared that the meat has exceeded the limit 4 for the maximum ratio n-6:n-3, which makes it unfit for human consumption. In the second experiment, featuring the legume main food of the diet, there is better quality for human consumption, with respect to n-6:n-3, still demonstrated that this treatment of the meat below the limit of 4.

Introdução: A ingestão de carboidrato (CHO) durante o exercício de resistência aeróbica de longa duração tem se mostrado capaz de otimizar o desempenho dos atletas. Alguns estudos recentes têm reportado também um efeito ergogênico quando a proteína (PTN) é adicionada a uma bebida carboidratada. Além disso, a coingestão tem sido relacionada com a atenuação do dano muscular, tido como fator limitante da recuperação muscular. No entanto, pouco se investigou sobre a influência dessas suplementações, comparadas a uma bebida placebo (PLA), no duatlo de distância olímpica. Objetivo: Comparar o efeito de três diferentes estratégias nutricionais de suplementação (CHO vs. CHO+PTN vs. PLA), durante um teste simulado de duatlo (TSD) olímpico, no desempenho e recuperação muscular de duatletas amadores. Métodos: Em um ensaio clínico, cruzado, randomizado e duplo-cego, treze atletas amadores do sexo masculino, com idade média de 29,7 ± 7,7 anos, participaram de três TSD consumindo: bebida carboidratada (CHO, 75 g); bebida isocalórica combinando carboidrato e proteína, na proporção 4:1 (CHO+PTN, 60,5 g CHO e 14,5 g PTN); e bebida placebo (PLA). Aos atletas informou-se apenas que o estudo visava investigar a influência de três diferentes tipos de suplementação. Após jejum de 8 h, sujeitos recebiam um café da manhã padrão, com 1,5 g/kg CHO e 45 min depois iniciavam o protocolo. As intensidades da primeira corrida (10 km) e da sessão de ciclismo (40 km) foram controladas, utilizando dados previamente coletados em testes preliminares, e a corrida final, de 5 km, foi tratada como um contrarrelógio (t5km). Coletas de sangue foram realizadas antes, imediatamente após e 24h após cada teste simulado, e analisou-se concentrações de glicose e creatina quinase (CK). Pico de torque (PT) isométrico foi mensurado no início do estudo e 24h após cada teste. Resultados: Os atletas completaram as distâncias totais do duatlo olímpico em ~1h 51min. Não houve diferença significativa no t5km entre as condições CHO (1270,3 ± 130,5 s) vs. CHO+PTN (1267,2 ± 138,9 s) vs. PLA (1275,4 ± 120 s); p = 0,87; TE ≤ 0,1. Os resultados de PT não demonstraram alterações significativas entre as condições basal (302,2 ± 52,8 N.m) vs. pós-24h CHO (300,1 ± 41,4 N.m) vs. pós-24h CHO+PTN (292,2 ± 49,4 N.m) vs. pós-24h PLA (282,1 ± 43,1 N.m); p = 0,24; TE ≤ 0,4. Embora, os resultados de CK tenham mostrado aumento significativo para todas as condições na comparação pré vs. pós-24h: CHO (300%; p < 0,01; TE = 0,93); CHO+PTN (82%; p < 0,01; TE = 0,73) e PLA (190%; p = 0,01; TE = 1,04), não foram encontradas diferenças entre as condições nos diferentes momentos - pré, imediatamente após e pós-24h (p = 0,32; TE = 0,3 – 1,04). Conclusão: Em uma prova simulada de duatlo olímpico, com refeição pré-teste contendo 1,5g/kg de carboidrato, as suplementações de CHO e de CHO+PTN não oferecem benefícios extras quando comparadas a uma bebida placebo no que diz respeito ao desempenho e recuperação muscular dos atletas. / Introduction: Carbohydrate (CHO) intake during long-term endurance exercise has been shown to optimize performance in athletes. Some recent studies have also reported an ergogenic effect when protein (PRO) is added to a carbohydrate drink. Furthermore, the coingestion has been related to the attenuation of muscle damage, seen as a limiting factor in muscle recovery. However, little has been investigated about the influence of such supplementation, compared to a placebo drink (PLA), in an Olympic-distance (OD) duathlon. Purpose: This study aimed to compare the effects of three different nutritional supplementation strategies (CHO vs. CHO+PRO vs. PLA) during a simulated OD duathlon, on performance and indices of muscle recovery of amateurs duathletes. Methods: In a crossover, randomized, double-blind clinical trial, thirteen male amateur athletes, mean age 29.7 ± 7.7 years, participated in three simulated OD duathlons consuming either a carbohydrate drink (CHO, 75 g); an isocaloric drink combining carbohydrate and protein in a 4:1 ratio (CHO+PRO, 60.5 g CHO and 14.5 g PRO); or a placebo (PLA) drink. The athletes were informed that the study aimed to investigate the influence of three different types of supplementation. After an 8 hours fast, subjects received a standard breakfast, with 1.5 g / kg CHO and 45 min later the protocol began. The intensities of the first running (10 km) and cycling session (40 km) were controlled using previously collected data, and the final running, 5 km, was treated as a time-trial (t5km). Blood samples were taken before, immediately after and 24 hours after each simulated duathlon and glucose and creatine kinase (CK) levels were analyzed. Isometric peak torque (PT) was measured at baseline and 24 hours after each test. Results: The athletes completed the OD duathlons in ~ 1h 51min. There was no significant difference in t5km between CHO (1270.3 ± 130.5 sec) vs. CHO+PRO (1267.2 ± 138.9 sec) vs. PLA (1275.4 ± 120 sec); p = 0.87; ES ≤ 0.1. The results of PT showed no significant changes between baseline conditions (302.2 ± 52.8 Nm) vs. 24h post-CHO (300.1 ± 41.4 Nm) vs. 24h post-CHO+PRO (292.2 ± 49.4 Nm) vs. 24h post-PLA (282.1 ± 43.1 Nm); p = 0.24; ES ≤ 0.4. Although CK results showed significant increase for all conditions when comparing pre vs. post 24h CHO (300%; p <0.01; ES = 0.93); CHO+PRO (82%; p <0.01; ES = 0.73) and PLA (190%, p = 0.01; ES = 1.04), no differences were found between the conditions in the different moments - pre, immediately after and after 24 hours (p = 0.32; ES = 0.3 – 1.04). Conclusion: In a simulated Olympic-distance duathlon, with pre-meal test containing 1.5g / kg of carbohydrate, the ingestion of CHO and CHO+PRO does not provide additional benefits when compared to a placebo drink regarding athletes’ performance and muscle recovery.

Made available in DSpace on 2016-12-06T17:06:54Z (GMT). No. of bitstreams: 1 Vanessa Schveitzer.pdf: 1403767 bytes, checksum: 300f03a4ead7ff7b06f9329650147535 (MD5) Previous issue date: 2012-03-15 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / This study aimed to investigate the effects of an acute dose of NAC as a possible modulator of a large number of markers of oxidative stress and tissue damage in volleyball athletes. Was conducted with 18 young athletes who performed their regular training without the use of antioxidant supplementation, where they underwent an intense training session of volleyball under the effect of Nac (1200mg) or placebo, and measured the biochemical markers before and after exercise. There were no significant changes in markers of oxidative stress and tissue damage. An interesting finding was observed that the amount of vitamin C showed high and may have masked the results of physical exercise and placebo. The ingestion of two doses of 600 mg of NAC is not sufficient to promote antioxidant and / or the protective tissue damage. / Este estudo objetivou investigar os efeitos de uma dose aguda de NAC, como possível modulador de um amplo número de marcadores de estresse oxidativo e de dano tecidual em atletas de voleibol. Foi realizado com 18 atletas juvenis que realizavam seu treinamento regular sem o uso de suplementação com antioxidantes, onde foram submetidos a uma sessão de treinamento intenso de voleibol, sob efeito de Nac (1200mg) ou placebo, sendo mensurados os marcadores bioquímicos pré e pós-exercício. Não houve alterações significativas nos marcadores de estresse oxidativo e de dano tecidual. Um dado interessante foi observado que a quantidade de Vitamina C apresentou elevada, podendo ter mascarado os resultados do exercício físico e placebo. A ingestão de duas doses de 600mg de NAC não é suficiente para promover efeito antioxidante e/ou protetivo a danos teciduais.

Introdução: A ingestão de carboidrato (CHO) durante o exercício de resistência aeróbica de longa duração tem se mostrado capaz de otimizar o desempenho dos atletas. Alguns estudos recentes têm reportado também um efeito ergogênico quando a proteína (PTN) é adicionada a uma bebida carboidratada. Além disso, a coingestão tem sido relacionada com a atenuação do dano muscular, tido como fator limitante da recuperação muscular. No entanto, pouco se investigou sobre a influência dessas suplementações, comparadas a uma bebida placebo (PLA), no duatlo de distância olímpica. Objetivo: Comparar o efeito de três diferentes estratégias nutricionais de suplementação (CHO vs. CHO+PTN vs. PLA), durante um teste simulado de duatlo (TSD) olímpico, no desempenho e recuperação muscular de duatletas amadores. Métodos: Em um ensaio clínico, cruzado, randomizado e duplo-cego, treze atletas amadores do sexo masculino, com idade média de 29,7 ± 7,7 anos, participaram de três TSD consumindo: bebida carboidratada (CHO, 75 g); bebida isocalórica combinando carboidrato e proteína, na proporção 4:1 (CHO+PTN, 60,5 g CHO e 14,5 g PTN); e bebida placebo (PLA). Aos atletas informou-se apenas que o estudo visava investigar a influência de três diferentes tipos de suplementação. Após jejum de 8 h, sujeitos recebiam um café da manhã padrão, com 1,5 g/kg CHO e 45 min depois iniciavam o protocolo. As intensidades da primeira corrida (10 km) e da sessão de ciclismo (40 km) foram controladas, utilizando dados previamente coletados em testes preliminares, e a corrida final, de 5 km, foi tratada como um contrarrelógio (t5km). Coletas de sangue foram realizadas antes, imediatamente após e 24h após cada teste simulado, e analisou-se concentrações de glicose e creatina quinase (CK). Pico de torque (PT) isométrico foi mensurado no início do estudo e 24h após cada teste. Resultados: Os atletas completaram as distâncias totais do duatlo olímpico em ~1h 51min. Não houve diferença significativa no t5km entre as condições CHO (1270,3 ± 130,5 s) vs. CHO+PTN (1267,2 ± 138,9 s) vs. PLA (1275,4 ± 120 s); p = 0,87; TE ≤ 0,1. Os resultados de PT não demonstraram alterações significativas entre as condições basal (302,2 ± 52,8 N.m) vs. pós-24h CHO (300,1 ± 41,4 N.m) vs. pós-24h CHO+PTN (292,2 ± 49,4 N.m) vs. pós-24h PLA (282,1 ± 43,1 N.m); p = 0,24; TE ≤ 0,4. Embora, os resultados de CK tenham mostrado aumento significativo para todas as condições na comparação pré vs. pós-24h: CHO (300%; p < 0,01; TE = 0,93); CHO+PTN (82%; p < 0,01; TE = 0,73) e PLA (190%; p = 0,01; TE = 1,04), não foram encontradas diferenças entre as condições nos diferentes momentos - pré, imediatamente após e pós-24h (p = 0,32; TE = 0,3 – 1,04). Conclusão: Em uma prova simulada de duatlo olímpico, com refeição pré-teste contendo 1,5g/kg de carboidrato, as suplementações de CHO e de CHO+PTN não oferecem benefícios extras quando comparadas a uma bebida placebo no que diz respeito ao desempenho e recuperação muscular dos atletas. / Introduction: Carbohydrate (CHO) intake during long-term endurance exercise has been shown to optimize performance in athletes. Some recent studies have also reported an ergogenic effect when protein (PRO) is added to a carbohydrate drink. Furthermore, the coingestion has been related to the attenuation of muscle damage, seen as a limiting factor in muscle recovery. However, little has been investigated about the influence of such supplementation, compared to a placebo drink (PLA), in an Olympic-distance (OD) duathlon. Purpose: This study aimed to compare the effects of three different nutritional supplementation strategies (CHO vs. CHO+PRO vs. PLA) during a simulated OD duathlon, on performance and indices of muscle recovery of amateurs duathletes. Methods: In a crossover, randomized, double-blind clinical trial, thirteen male amateur athletes, mean age 29.7 ± 7.7 years, participated in three simulated OD duathlons consuming either a carbohydrate drink (CHO, 75 g); an isocaloric drink combining carbohydrate and protein in a 4:1 ratio (CHO+PRO, 60.5 g CHO and 14.5 g PRO); or a placebo (PLA) drink. The athletes were informed that the study aimed to investigate the influence of three different types of supplementation. After an 8 hours fast, subjects received a standard breakfast, with 1.5 g / kg CHO and 45 min later the protocol began. The intensities of the first running (10 km) and cycling session (40 km) were controlled using previously collected data, and the final running, 5 km, was treated as a time-trial (t5km). Blood samples were taken before, immediately after and 24 hours after each simulated duathlon and glucose and creatine kinase (CK) levels were analyzed. Isometric peak torque (PT) was measured at baseline and 24 hours after each test. Results: The athletes completed the OD duathlons in ~ 1h 51min. There was no significant difference in t5km between CHO (1270.3 ± 130.5 sec) vs. CHO+PRO (1267.2 ± 138.9 sec) vs. PLA (1275.4 ± 120 sec); p = 0.87; ES ≤ 0.1. The results of PT showed no significant changes between baseline conditions (302.2 ± 52.8 Nm) vs. 24h post-CHO (300.1 ± 41.4 Nm) vs. 24h post-CHO+PRO (292.2 ± 49.4 Nm) vs. 24h post-PLA (282.1 ± 43.1 Nm); p = 0.24; ES ≤ 0.4. Although CK results showed significant increase for all conditions when comparing pre vs. post 24h CHO (300%; p <0.01; ES = 0.93); CHO+PRO (82%; p <0.01; ES = 0.73) and PLA (190%, p = 0.01; ES = 1.04), no differences were found between the conditions in the different moments - pre, immediately after and after 24 hours (p = 0.32; ES = 0.3 – 1.04). Conclusion: In a simulated Olympic-distance duathlon, with pre-meal test containing 1.5g / kg of carbohydrate, the ingestion of CHO and CHO+PRO does not provide additional benefits when compared to a placebo drink regarding athletes’ performance and muscle recovery.

Orientador: Patrícia Moriel / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-08-26T00:37:20Z (GMT). No. of bitstreams: 1 Freitas_BetaniadeJesuseSilvadeAlmendra_D.pdf: 2657931 bytes, checksum: d4646bbc60ccc13e11ca7d806b4f75dc (MD5) Previous issue date: 2014 / Resumo: Estudos acerca da influência do estresse oxidativo sobre o equilíbrio cutâneo, sobretudo por seus efeitos devastadores sobre a integridade da pele, são essenciais para a proposição de estratégias de intervenção preventivas para o desenvolvimento do câncer de pele. O objetivo do estudo foi comparar o estresse oxidativo de indivíduos que tiveram e não tiveram câncer de pele não melanoma e avaliar o efeito da suplementação combinada de vitaminas C, E e mineral Zinco no estresse oxidativo de indivíduos que apresentaram a doença. O estudo foi dividido em duas fases: a fase 1 foi um estudo transversal com controles, cuja população foi constituída por pessoas saudáveis (n = 24) e o grupo caso constituído por indivíduos que apresentaram câncer de pele não melanoma já submetidas a tratamento cirúrgico (n = 60). E a fase 2, um ensaio clínico randomizado e duplo cego, no qual os pacientes do grupo caso foram randomizados em dois subgrupos: grupo placebo (n = 34) e grupo suplementado (n = 26) com 50 mg de vitamina C, 60 mg de vitamina E e 40 mg de Zinco durante 8 semanas. As amostras de sangue dos sujeitos foram obtidas no período basal e após intervenção para a avaliação dos biomarcadores de estresse oxidativo (F2-isoprostano, nitrito, substâncias reativas ao ácido tiobarbitúrico (TBARS) e capacidade antioxidante total). O consumo alimentar habitual e o estado nutricional dos sujeitos foram avaliados. Para identificação dos fatores associados ao câncer de pele foi utilizada a análise de regressão logística univariada e multivariada. O nível de significância adotado para este estudo foi de 5%. A maioria dos pacientes estudados foram do sexo feminino com idade superior a 50 anos. Os pacientes do grupo caso apresentaram mais elevadas concentrações séricas dos biomarcadores de estresse oxidativo, sendo que as concentrações de F2-isoprostano estavam significativamente mais elevadas em comparação com os controles. Após suplementação não houve diferença estatística entre os grupos placebo e suplementado em relação aos marcadores de estresse oxidativo. A idade e o F2-isoprostano podem ser marcadores de risco para o câncer de pele não melanoma, a cada ano a mais para o fator idade aumenta em 12% a chance de câncer e cada unidade a mais na medida do marcador aumenta em 4% a chance de câncer. Os resultados mostraram prevalência de sobrepeso no grupo controle com diferença estatística significativa em relação ao grupo caso. As concentrações dietéticas dos minerais antioxidantes zinco, cobre e selênio do grupo caso foram estatisticamente inferiores em relação aos controles e não houve diferença estatística nas concentrações dietéticas dos nutrientes antioxidantes entre os grupos suplementado e placebo. Este estudo sugere que pessoas diagnosticadas com câncer de pele não melanoma e que no momento da realização da pesquisa não mais apresentavam a doença, mostravam elevado estresse oxidativo, quando comparadas a pessoas saudáveis. A suplementação de antioxidantes pelo período de tempo realizado no trabalho não provocou redução significativa nas concentrações dos marcadores de estresse oxidativo dos pacientes. O estudo ainda sugere que o marcador de estresse oxidativo F2-isoprostano pode ser utilizado como um fator de risco para o desenvolvimento do câncer de pele não melanoma / Abstract: Studies investigating the influence of oxidative stress on skin homeostasis, especially for its devastating effects on skin integrity, are essential for the development of preventive intervention strategies for skin cancer. The goal of this study was to compare the concentrations of oxidative stress biomarkers in blood between individuals with and without non-melanoma skin cancer and evaluate the effect of combined supplementation with vitamins C, E, and the mineral zinc on oxidative stress in skin-cancer patients. The study was divided into two stages: stage 1 was cross-sectional study with controls, whose population consisted of healthy individuals (n = 24) and the case group included individuals who had non-melanoma skin cancer undergoing surgery (n = 60). And the second phase a randomized, double blind clinical trial where patients in the case group were randomized into two subgroups: placebo (n =34) and a supplemented group (n = 26) who received 50 mg of vitamin C, 60 mg of vitamin E, and 40 mg of zinc for 8 wk. Blood samples were taken from the subjects before and after intervention to evaluate levels of oxidative stress biomarkers (F2-isoprostane, nitrite, thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity. The usual food consumption and nutritional state of the subjects were also evaluated. Multivariate and univariate logistics regression analysis were used to identify factors associated with the development of skin cancer. The level of significance adopted for this study was 5%. The majority of participants were women over the age of 50. The patients in the case group had higher serum concentrations of oxidative stress biomarkers, and the levels of F2-isoprostane were significantly higher than the controls. After antioxidant supplementation there was no statistical difference in the markers of oxidative stress among the placebo and supplemented groups. Age and F2-isoprostane may be effective biomarkers for estimating the risk of non-melanoma skin cancer development. Moreover, the risk of cancer increases with age at a rate of 12% per year, while an increase in concentration of these biomarker in blood increases the risk of cancer by 4%. These results showed a prevalence of excess weight in the control group with significant statistical difference from the case group. The dietary intake of the mineral antioxidants zinc, copper, and selenium of the case group were significantly lower than the control group, and there was no statistical difference in the dietary intake of the antioxidant nutrients among the supplemented and placebo groups. This study suggests that people diagnosed with non-melanoma skin cancer and those in remission at the time of the study, exhibited higher concentration oxidative stress than healthy individuals. The antioxidant supplementation by period the work performed did not cause significant reduction in serum concentrations of oxidative stress biomarkers of the patients. The results suggest that the concentration of the oxidative stress biomarker, F2-isoprostane, may serve as risk factor for non-melanoma skin cancer development / Doutorado / Ciencias Biomedicas / Doutora em Ciências Médicas

Made available in DSpace on 2014-08-20T13:49:12Z (GMT). No. of bitstreams: 1 Gesiane Teixeira.pdf: 1665700 bytes, checksum: 868caf8c69bf5cf7a1cac6d5eb50ea3c (MD5) Previous issue date: 2013-02-22 / Introduction: The growing number of fitness centers is followed by an increased consumption of ergogenic supplements by clients and professionals who attend these places. The present study aimed to describe the nutritional profile and the use of ergogenic aids in fitness centers workers. Methods: We conducted a population-based cross-sectional study in all gyms of Pelotas, southern Brazil. Was assessed from standardized questionnaire, the consumption of ergogenic supplements (protein, carbohydrate, anticatabolic, multivitamins and hormonal), markers of dietary intake, frequency of fruit and vegetable intake and body mass index (BMI). In addition, we collected sociodemographic and work variables. Results: Mean age of the 497 respondents was 29.7 years (± 8.3), 57.8% were male and most were in the normal BMI range. Almost a quarter of men and less than 10% of women reported consuming at least one type of supplement. As for food intake, we observed a high frequency of consumption of protein-based products, burgers/sausages and soft drinks. The most reported ergogenic was the anticatabolic (75.4% of men and 71.8% women consumed this type of supplement). The higher fruit intake was reported (40.3%) among respondents who consumed only one supplement and vegetable consumption was more common among those consuming two supplements (45.1%). Conclusions: The population assessed, which is often taken as an example by fitness clubs participants, showed a nutritional profile that could be considered inappropriate, where 25% make use of ergogenic aids, showed a high frequency of consumption of protein and soft drinks. In addition, the intake of fruits and vegetables was below the recommendations and inversely proportional to the consumption of supplements / Atualmente se destaca o crescente número de academias de ginástica e o expressivo aumento no consumo de suplementos ergogênicos por alunos e profissionais que frequentam esses locais. O presente estudo objetivou descrever o perfil nutricional e o uso de recursos ergogênicos de trabalhadores de academias de ginástica que ministravam atividade física. Métodos: Estudo descritivo observacional transversal do tipo censo realizado nas academias de ginástica da cidade de Pelotas/RS. Foi avaliado, a partir de questionário padronizado, o consumo de suplementos ergogênicos (proteicos, carboidratados, anticatabólicos, polivitamínicos e hormonais), marcadores de consumo alimentar, frequência de ingestão de frutas e vegetais e índice de massa corporal (IMC). Além disso, foram coletadas variáveis sociodemográficas e do trabalho. Resultados: Foram entrevistados 497 profissionais com média de idade referente a 29,7 anos (± 8,3), 57,8% eram do sexo masculino e a maioria tinha IMC de eutrofia (56,3%). Cerca de 25% dos homens e 10% das mulheres reportaram consumir algum tipo de suplemento. Quanto aos marcadores de consumo alimentar, pode-se observar a alta frequência de consumo de produtos protéicos, hambúrgueres/embutidos e refrigerantes. O recurso ergogênico mais relatado foi o anticatabólico, sendo que 75,4% dos homens e 71,8% das mulheres consumiram esse tipo de suplemento. O consumo de frutas foi mais relatado (40,3%) entre os entrevistados que consumiam apenas um suplemento e o consumo de vegetais foi mais frequente entre os consumidores de dois suplementos (45,1%). Conclusões: A população avaliada, que com frequência é tomada como exemplo por frequentadores de academias, mostrou um perfil nutricional que pode ser considerado inadequado, onde 25% faz uso de recursos ergogênicos, mostrou elevada frequência de consumo de proteínas e refrigerantes. Somado a isso, o consumo de frutas e vegetais foi aquém das recomendações e inversamente proporcional ao consumo de suplementos