Die missbräuchliche Anwendung von anabolen Substanzen erfolgt mit dem Ziel eines verstärkten Muskelaufbaus - im Sport zur Leistungsverbesserung, in der Tierzucht zum Erreichen von Zuchtidealen oder bei der Masttierhaltung zur Produktivitätssteigerung. Bisher wurden Doping- oder Medikationskontrollen zum Nachweis von anabolen Steroidhormonen üblicherweise im Urin bzw. im Blut durchgeführt. Für bestimmte Fragestellungen kann der analysierbare Zeitraum allerdings unzureichend sein oder aber die Untersuchungsmaterialien sind unter praktischen Gegebenheiten nur eingeschränkt verfügbar. Das Sammeln von Urinproben ist beispielsweise bei Zuchthengsten nur mit einem unverhältnismäßig hohen Aufwand realisierbar. Haare stellen in solchen Situationen eine Alternative dar, da sich das Entnahmeverfahren unkompliziert gestaltet und bei einer entsprechenden Haarlänge die eingelagerten Fremdstoffe länger als in Urin- oder Blutproben detektierbar sein sollten. In der vorliegenden Arbeit wurde ein effektiver Langzeitnachweis für insgesamt 11 anabole Substanzen in Pferdehaar-Proben mittels GC-HRMS und GC-MS/MS entwickelt (Nachweisgrenzen zwischen 0,1 und 5,0 pg/mg). Dabei können zum einen körperfremde anabole Wirkstoffe (z. B. Steroidester in Depotpräparaten) und zum anderen körper-eigene Steroide analysiert werden (z. B. Testosteron und Nandrolon beim Hengst). In verschiedenen Applikationsversuchen wurde gezeigt, dass durch eine Haaranalyse der Nachweis bis zu einem Jahr möglich ist. Für die endogene Nandrolonmenge in Schweifproben von unbehandelten Hengsten wurde eine signifikante Altersabhängigkeit festgestellt. Die ermittelten physiologischen Höchstkonzentrationen für Nandrolon betragen zwischen 1,1 pg/mg bei Junghengsten (1-3 Jahre) und 3,1 pg/mg bei Althengsten (11-20 Jahre). Die Bestimmung von Nandrolon in Haarproben erwies sich für die Körungskontrollen bei Junghengsten als ein geeignetes Verfahren zur Detektion einer exogenen Zufuhr. Die Untersuchung von Haaren ist zum Langzeitnachweis als Alternative gegenüber Blut- und Urinanalysen vorzuziehen, auch wenn sich retrospektiv nicht alle Fragen zum Behandlungsablauf präzise klären lassen (z. B. Angaben zur Dosierung oder zum genauen Applikationszeitpunkt). Das neu etablierte Verfahren ist außerdem die Methode der Wahl, wenn die Verfügbarkeit der übrigen Probematerialien eingeschränkt bzw. eine einfache und schnelle Beprobung erforderlich ist. Es wird bereits zur Medikationskontrolle bei Zuchthengsten sowie bei speziellen forensischen Untersuchungen eingesetzt.

Made available in DSpace on 2014-08-20T13:32:48Z (GMT). No. of bitstreams: 1 tese_thais_farias_collares.pdf: 1094762 bytes, checksum: 647dd933472bf9127c0164ee875617ea (MD5) Previous issue date: 2013-10-17 / The history of sports competition has been related to the use of methods for physical training associated with physiological methods to increase athlete performance. Ethical issues involving the high performance sport and doping are confused with the history of competitive sports. The World Anti - Doping Agency (WADA) prohibits the use of substances or methods to artificially increase sports performance. Currently, 258 substances are listed by WADA and athletes around the world are subjected to tests proving the non-use of doping. EPO is a glycoprotein hormone that has as its main physiological effect induction of erythropoiesis and thereby improving the capacity to transport oxygen in the blood. For these reason EPO has been included in WADA list. The analytical differentiation of endogenous erythropoietin from its recombinant counterpart, using isoelectric focusing and double blotting is a milestone in the detection of doping with recombinant erythropoietin. However, several analogs of the original recombinant EPO are not easily detectable by standard IEF method, requiring the development of alternatives for the detection of doping. In order to improve the current methods of EPO detection, monoclonal and polyclonal antibodies against EPO were obtained and used in various techniques for detection of EPO in biological samples. However, the specificity of these antibodies has been quite controversial and discussed. Therefore, it is necessary to obtain antibodies capable of reacting specifically with the EPO. In this study, rHuEPO was inoculated in New Zealand rabbits to generate a polyclonal antibody (pAb anti-rHuEPO). The pAb was characterized for its potential in detecting rHuEPO using different approaches. The pAb anti- rHuEPO identified the expression of recombinant protein in eukaryotic cells and was able to detect rHuEPO in a suspension at 0.1 μg/mL, showing its potential as a tool for detection of doping by rHuEPO. / A história da competição esportiva sempre esteve relacionada à utilização de metodologias de treinamento físico associadas a métodos de incremento fisiológico do atleta, visando sua máxima performance. Questões éticas envolvendo o esporte de alto rendimento e o doping se confundem com a própria história do esporte competitivo. A Agência Mundial Anti-Doping (WADA) proíbe o uso de substâncias ou métodos capazes de aumentar artificialmente o desempenho esportivo. Atualmente, 258 substâncias estão na lista da WADA e atletas do mundo inteiro são submetidos a testes comprobatórios da não utilização do doping. Entre essas substâncias ilícitas para uso por atletas destaca-se a eritropoetina (EPO). A EPO é um hormônio glicoproteico que possui como principal efeito fisiológico a indução da eritropoiese e consequente melhoria da capacidade de transporte de oxigênio no sangue. A diferenciação analítica da eritropoetina endógena produzida a partir de sua contraparte recombinante usando focalização isoelétrica e duplo blotting é um marco na detecção do doping com eritropoetina recombinante. Anticorpos monoclonais e policlonais específicos anti-EPO foram obtidos e utilizados em várias técnicas para a detecção da EPO em amostras biológicas a fim de melhorar os métodos atuais de detecção. Contudo, a especificidade destes anticorpos tem sido bastante controversa e discutida. Portanto, a obtenção de anticorpos específicos capazes de reagir especificamente com a EPO faz-se necessária. Neste estudo, a rHuEPO foi utilizada para imunizar coelhos New Zealand para gerar um anticorpo policlonal (pAb anti-rHuEPO). O pAb foi caracterizado quanto ao seu potencial na detecção da rHuEPO usando diferentes metodologias. O pAb anti-rHuEPO identificou a expressão da proteína recombinante em células eucarióticas e foi capaz de detectar rHuEPO em suspensão até 0,1 μg/mL, comprovando seu potencial como ferramenta para detecção do doping por rHuEPO.

Made available in DSpace on 2014-08-20T13:32:56Z (GMT). No. of bitstreams: 1 dissertacao_thais_farias_collares.pdf: 421000 bytes, checksum: d922362fc9456c8a75d15a49744bf882 (MD5) Previous issue date: 2010-05-28 / The administration of recombinant proteins is being used in sport as gene doping. In medicine, a recent therapeutic technique is the genetic therapy, which, up to this moment, shows results that indicate its efficiency in the treatment of some diseases. Recently, the potential for misuse of gene therapy among athletes has called the attention of scientists and sports regulating organs. The transfer of genes that could enhance athletic performance was named gene doping. The most important candidate genes for gene doping are Erythropoietin (EPO), vascular endothelial growth factor (VEGF), insulin-like growth factor I (IGF-1) and myostatin blockers. Nevertheless, gene therapy presents adverse indicators, such as inflammatory response and lack of control of gene activation. It is probable that in healthy individuals such problems would be aggravated. There are still no conclusive tests capable of detecting gene doping. However, recent researches have studied promising strategies. The reflection of the use of EPO on reproductive parameters in vivo has not yet been described. On the other hand, in vitro studies with cultured cells have shown that erythropoietin stimulates steroidogenesis in Leydig cells, triggering an increase in testosterone production. The objective of this study is to evaluate the effects of the administration of recombinant erythropoietin (rHuEpo) and erythropoietin gene transfer in reproductive parameters of rabbits. Fifteen rabbits were divided in 3 groups: group I (rHuEpo) received subcutaneously 25UI/kg of recombinant human erythropoietin, three times a week for 5 weeks; group II (pTarget/Epo) received a single dose of recombinant vector with the gene of the rabbit erythropoietin; group III (pTarget) received a single dose of empty pTarget vector (control). Throughout the experiment, reproductive and blood parameters were monitored, such as: sperm motility, spermatic vigor, sperm concentration, sperm viability, sperm morphology, erythrocytes and hematocrit level. Erythropoietin gene transfer and rHuEpo administration caused a significant increase in the number of erythrocytes. The animals which received rHuEpo showed an increase in the hematocrit level, reaching numbers between 41,34 and 52,32. The statistical analysis proved that the treatment and the time did not interfer on sperm motility, sperm concentration and spermatic vigor (P<0.05). The percentage of morphologically normal cells in group I as well as in group II decreased over time, however, there was no statistical difference between the treatments (P<0.05). This study is the first to show the answers to the use of gene doping with the erythropoietin gene and the rHuEpo administration in reproductive and blood parameters. / A administração de proteínas recombinantes vem sendo utilizada no esporte como um meio de doping. Na medicina, um método terapêutico bastante recente é a terapia gênica, que até o momento, possui resultados indicando sua eficiência no tratamento de algumas doenças. Recentemente, o potencial para uso indevido desta terapia entre atletas tem despertado a atenção de cientistas e órgãos reguladores do esporte. A transferência de genes que poderiam melhorar o desempenho esportivo de atletas saudáveis foi denominada de doping genético. Os principais genes candidatos são eritropoetina (EPO), fator de crescimento endotelial vascular (VEGF), fator de crescimento semelhante à insulina tipo 1 (IGF-1) e bloqueadores da miostatina. Porém a terapia gênica apresenta indicadores adversos, como resposta inflamatória e falta de controle da ativação do gene. Em indivíduos saudáveis, é provável que essa situação seja agravada. Ainda não existem testes conclusivos para a detecção do doping genético, no entanto alguns estudos recentes têm o intuito de investigar algumas estratégias que apontam como promissoras. O reflexo do uso da EPO sobre parâmetros reprodutivos in vivo ainda não tem sido descritos, por outro lado, estudos in vitro com cultivo de células têm demonstrado que a eritropoetina estimula a esteroidogênese nas células de Leydig desencadeando um aumento na produção de testosterona. O objetivo deste estudo foi avaliar os efeitos da administração de rHuEpo (eritropoetina recombinante) e da transferência gênica com eritropoetina em parâmetros reprodutivos de coelhos. Quinze coelhos foram divididos em 3 grupos: grupo I (rHuEpo) receberam por via subcutânea 25UI/kg de eritropoetina humana recombinante, três vezes por semana durante cinco semanas, grupo II (pTarget/Epo) receberam dose única de vetor recombinante com o gene da eritropoetina de coelho; grupo III (pTarget) receberam dose única de vetor pTarget vazio (controle). Parâmetros sanguíneos e reprodutivos foram monitorados durante o experimento, tais como: motilidade, vigor espermático, concentração espermática, viabilidade espermática, morfologia espermática, hemácias e hematócrito. A transferência gênica com eritropoietina e a administração de rHuEpo causaram um aumento significativo no número de eritrócitos. Os animais que receberam rHuEpo obtiveram um aumento no hematócrito, alcançando valores entre 41,34 e 52,32. A análise estatística mostrou que os tratamentos e o tempo não interferiram na motilidade, concentração espermática e vigor espermático (P <0,05). A porcentagem de células com morfologia normal, tanto do grupo I como do grupo II diminuiu no decorrer do tempo, mas não houve diferença estatística entre os tratamentos (P<0,05). Este é o primeiro estudo que relata as respostas do uso do doping genético com o gene da eritropoetina e da administração de rHuEpo em parâmetros sanguíneos e reprodutivos.

Peer reviewed literature currently provides little knowledge of how to recruit and train Doping Control Officers (DCOs) for Mega Sport Events. This study adds to the existing body of literature on creating knowledge transfer and legacy for future Mega Event Organizing Committees. A phenomenological designed study was conducted through the use of a qualitative on-line survey during the 2015 Toronto Pan American Games. A survey response rate of 58% was achieved. Survey responses by DCOs were analyzed using inductive coding to identify four main themes; i) DCO motivations, ii) training program design, iii) engagement and support, and iv) barriers to participation in the results. Recommendations include using mixed methods for future studies, provide DCOs with training and evaluation opportunities prior to the Mega Sport Event, and developing mentorship opportunities during games operations. Additional research is needed to create greater understanding of DCO motivations and perceptions beyond this study.

Made available in DSpace on 2016-01-26T18:55:41Z (GMT). No. of bitstreams: 1 Yona Murad.pdf: 235020 bytes, checksum: 9b83ae7421537fd5e72802ad16c29b88 (MD5) Previous issue date: 2014-09-26 / In order to determine the hematologic changes resulting from the use of rhGH (recombinant human growth hormone), associated or not with physical training in rats, eighty Wistar rats, average weight of 211.8 g were randomly divided into four groups (n=20): CT (control group without physical training and without administration of rhGH), GH (group without physical training and with rhGH), TR (group with physical training and without rhGH) and TRGH (physical training group with rhGH). The animals in groups GH and TRGH received 0.2 IU/kg rhGH subcutaneously every two days during the 30 day period. Physical training was performed using a jumping protocol consisting of four sets of ten jumps with overload of 50% of body weight, three days a week, in a cylindrical container with 38 cm of heated water (30° C). At the end of 4 weeks, the animals were weighed, anesthetized and then killed by exsanguination for collection of blood for analyses. The following parameters were evaluated: number of erythrocytes, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, RDW (Red Cell Distribution Width), and leukocyte count. Red blood cell parameters did not change with exercise training and / or hormonal supplementation. In all groups RDW remained unchanged. The leukocyte count of the animals that have done physical training and were supplemented with hormone was higher when compared to sedentary rats (P < 0.05). The administration of rhGH at a dose of 0.2 IU/kg associated with exercise training increased the number of leukocytes, but did not change the remaining parameters of the hemogram in sedentary and active rats. It was not possible to detect an increase in erythropoiesis, through the use of rhGH, using these variables. / Com o objetivo de verificar as alterações hematológicas decorrentes do uso do rhGH (hormônio do crescimento recombinante humano), associado ou não ao treinamento físico, foram utilizados 80 ratos da linhagem Wistar, peso médio de 211,8 g que foram distribuídos aleatoriamente em quatro grupos (n=20): CT (grupo controle sem treinamento físico e sem administração de rhGH), GH (grupo sem treinamento físico e com rhGH), TR (grupo com treinamento físico e sem rhGH) e TRGH (grupo com treinamento físico e com rhGH). Os animais dos grupos GH e TRGH receberam 0,2 UI/kg de rhGH por via subcutânea a cada dois dias durante o período de 30 dias. O treinamento físico foi realizado por meio de protocolo de salto composto de quatro séries de dez saltos, com sobrecarga de 50% do peso corpóreo, durante três dias na semana, em um recipiente cilíndrico com 38 cm de água aquecida (30°C) em seu interior. Ao final de 4 semanas, os animais foram pesados, anestesiados e então mortos por exsanguinação para colheita do sangue para a realização do hemograma. Foram avaliados os seguintes parâmetros: número de eritrócitos, hemoglobina, hematócrito, volume corpuscular médio, concentração de hemoglobina corpuscular média, RDW (Red Cell Distribution Width) e contagem de leucócitos totais. As variáveis do eritrograma não sofreram alterações com o treinamento físico e/ou suplementação hormonal. Em todos os grupos, os valores de RDW permaneceram inalterados. A contagem de leucócitos dos animais que fizeram treinamento físico e foram suplementados com hormônio foi maior quando comparados aos sedentários (P<0,05). Conclui-se que a administração do rhGH na dose de 0,2 UI/kg associado ao treinamento físico induziu a leucocitose, porém não influenciou os parâmetros da série vermelha tanto em animais sedentários como ativos. Não foi possível, a partir destas variáveis, detectar estímulo na eritropoiese destes animais.

Made available in DSpace on 2016-07-18T17:53:13Z (GMT). No. of bitstreams: 1 Yona Murad.pdf: 235020 bytes, checksum: 9b83ae7421537fd5e72802ad16c29b88 (MD5) Previous issue date: 2014-09-26 / In order to determine the hematologic changes resulting from the use of rhGH (recombinant human growth hormone), associated or not with physical training in rats, eighty Wistar rats, average weight of 211.8 g were randomly divided into four groups (n=20): CT (control group without physical training and without administration of rhGH), GH (group without physical training and with rhGH), TR (group with physical training and without rhGH) and TRGH (physical training group with rhGH). The animals in groups GH and TRGH received 0.2 IU/kg rhGH subcutaneously every two days during the 30 day period. Physical training was performed using a jumping protocol consisting of four sets of ten jumps with overload of 50% of body weight, three days a week, in a cylindrical container with 38 cm of heated water (30° C). At the end of 4 weeks, the animals were weighed, anesthetized and then killed by exsanguination for collection of blood for analyses. The following parameters were evaluated: number of erythrocytes, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, RDW (Red Cell Distribution Width), and leukocyte count. Red blood cell parameters did not change with exercise training and / or hormonal supplementation. In all groups RDW remained unchanged. The leukocyte count of the animals that have done physical training and were supplemented with hormone was higher when compared to sedentary rats (P < 0.05). The administration of rhGH at a dose of 0.2 IU/kg associated with exercise training increased the number of leukocytes, but did not change the remaining parameters of the hemogram in sedentary and active rats. It was not possible to detect an increase in erythropoiesis, through the use of rhGH, using these variables. / Com o objetivo de verificar as alterações hematológicas decorrentes do uso do rhGH (hormônio do crescimento recombinante humano), associado ou não ao treinamento físico, foram utilizados 80 ratos da linhagem Wistar, peso médio de 211,8 g que foram distribuídos aleatoriamente em quatro grupos (n=20): CT (grupo controle sem treinamento físico e sem administração de rhGH), GH (grupo sem treinamento físico e com rhGH), TR (grupo com treinamento físico e sem rhGH) e TRGH (grupo com treinamento físico e com rhGH). Os animais dos grupos GH e TRGH receberam 0,2 UI/kg de rhGH por via subcutânea a cada dois dias durante o período de 30 dias. O treinamento físico foi realizado por meio de protocolo de salto composto de quatro séries de dez saltos, com sobrecarga de 50% do peso corpóreo, durante três dias na semana, em um recipiente cilíndrico com 38 cm de água aquecida (30°C) em seu interior. Ao final de 4 semanas, os animais foram pesados, anestesiados e então mortos por exsanguinação para colheita do sangue para a realização do hemograma. Foram avaliados os seguintes parâmetros: número de eritrócitos, hemoglobina, hematócrito, volume corpuscular médio, concentração de hemoglobina corpuscular média, RDW (Red Cell Distribution Width) e contagem de leucócitos totais. As variáveis do eritrograma não sofreram alterações com o treinamento físico e/ou suplementação hormonal. Em todos os grupos, os valores de RDW permaneceram inalterados. A contagem de leucócitos dos animais que fizeram treinamento físico e foram suplementados com hormônio foi maior quando comparados aos sedentários (P<0,05). Conclui-se que a administração do rhGH na dose de 0,2 UI/kg associado ao treinamento físico induziu a leucocitose, porém não influenciou os parâmetros da série vermelha tanto em animais sedentários como ativos. Não foi possível, a partir destas variáveis, detectar estímulo na eritropoiese destes animais.

Introduction: The use of doping has become more normal outside of performance sports. People working out at the gym focus on creating a perfect body image. Social media shows us how we should look or eat to become more accepted by society. The female body image has changed over the years. Now it is more normal for women to have muscles but it is still important to remain their curves. Purpose: The purpose with this study is to get at deeper understanding how female weight lifters experience doping in their gym environment. We even look into how and if the body image affects women`s tolerance against doping. Methodology: The study uses a qualitative approach. Collection of data is done by personal and separate interviews. Results: The majority sees a connection between the female body image and the use of doping but thinks it is more common that young girl get affected. The participants have a negative attitude against doping and considers it as cheating. They mention that there is a fear of side effects. / Inledning: Användning av doping har blivit mer vanligt utanför elitidrotten Mycket kan handla  om att skapa en viss idealkropp. Sociala medier och likande visar upp bilder på hur kroppen ska vara byggd för att kunna bli accepterad i samhället. Den kvinnliga kroppsidealen har förändrat sig de senaste åren. Nu är det mer vanligt att kvinnor ska ha muskler men även bevara sina kurvor. Syfte: Syftet med denna studie är att få en djupare förståelse för den kvinnliga styrketränarens uppfattning och upplevelse av doping i vårt nuvarande samhälle. Studien undersöker enbart kvinnors syn och attityd mot doping i sin gymmiljö och hur de upplever att samhällets kroppsideal påverkar den. Metod: Studien använder sig av en kvalitativ forskningsmetod. Datainsamlingen sker via enskilda semistruktuerade intervjuer. Som hjälpmedel används det en intervjuguide. Resultat: De flesta deltagarna kan se en koppling mellan kroppsidealen och doping hos kvinnor men tror att det påverkar mer yngre tjejer. Deltagarna anser doping som fusk och har en negativ inställning mot det. Även en rädsla för biverkningar nämns.

<p>In transformers used in the electrical industry, a coating, such as magnesium oxide or magnesia (MgO), is needed to coat the magnetic ferrite core, such as silicon steel. The coating is to provide electrical insulation of the layers of the ferrite core material, in order to reduce its heat dissipation loss. The coating also separate the layers of the coiled materials to prevent their sticking or welding during high temperature uses. </p> <p> </p> <p>The goal of this thesis is to perform a modeling study to understand the mechanical, thermodynamic, magnetic and thermal properties of pure and M-doped (M stands for Mn, Co, or Ni) magnesia, thus providing a theoretical understanding of the application of this group of coating materials for transformer applications. </p> <p> </p> <p>The study has the following sections. The first section is focused on the mechanical properties of pure magnesia. Using density functional theory (DFT) based calculations, the computed Young’s modulus, Poisson’s ratio, bulk modulus, and compressibility are 228.80 GPa, 0.2397, 146.52 GPa, and 0.00682, respectively, which are in good agreement with the literature data. Using molecular dynamics (MD) simulations, the computed Young’s modulus is 229 GPa. Using discrete element model (DEM) approach, the bending deformation of magnesia is simulated. Finally, using finite element model (FEM), micro-hardness indentation of magnesia is simulated, and the computed Brinell hardness is 16.1 HB, and Vickers hardness is 16 GPa.</p> <p> </p> <p>The second section is on the thermodynamic and physical properties of pure and doped magnesia. Using DFT based simulations, the temperature-dependent thermodynamic properties, such as free energy, enthalpy, entropy, heat capacity at constant volume, and Debye temperature of magnesia, are computed. The X-ray powder diffraction (XRD) spectra of M-doped magnesia are simulated, at the doping level of 1.5%, 3%, 6% and 12%, respectively. The simulated XRD data show that peaks shift to higher angles as the doping level increases. </p> <p> </p> <p>The third section is on the magnetic properties of pure and doped magnesia. Using DFT based simulations, the calculated magnetic moments increase with the doping level, with Mn as the highest, followed by Co and Ni. This is due to the fact that Mn has more unpaired electrons than Co and Ni. </p> <p> </p> The fourth section is on the thermal properties of the pure magnesia. Using the Reverse Non-Equilibrium Molecular Dynamics (RNEMD) method, the computed thermal conductivity of magnesia is 34.63 W/m/K, which is in agreement with the literature data of 33.0 W/m/K at 400 K.

Syftet med föreliggande uppsats är att undersöka hur doping och anti-doping förstås i ett gym- och fitnessammanhang. För att besvara syftet formulerades tre frågeställningar angående förekomsten av doping, hur doping kan förstås i relation till kropp och genus, och om anti-doping och preventiva insatser. Utifrån semistrukturerade intervjuer tillsammans med nio informanter, tillhörande bodybuildingens professionella sfär, har sedan frågeställningarna försökt besvarats. Informanterna bestod av tre kvinnor och sex män i åldrarna 21 till 45 år. Det empiriska materialet har analyserats utifrån Connells begrepp hegemonisk maskulinitet och betonad femininitet, samt delar av Bourdieus begreppsapparat. Resultatet presenteras under tre olika teman som berör respektive frågeställning. Utifrån resultatet kan doping förstås vara vanligt förekommande inom professionell bodybuilding vid tävlingssatsning. Doping förekommer även utanför tävlingskontext hos användare med motiv som på olika sätt förankras i rekreation och estetik. I båda fallen kan doping förstås i syfte att stå ut och skapa ett narrativ om att vara något utöver vanliga. Resultatet visar även att anti-dopingarbetet är svårnavigerat och delvis splittrat där nya perspektiv på arbetssätt möter äldre, och där anti-dopingrörelsens olika aktörer kan behöva göras enhetliga för att inte riskera sin trovärdighet. / The purpose of this essay is to examine (anti)doping in a gym- and fitness environment. To answer the research questions, semi-structured interviews have been accomplished with members all part of the professional bodybuilding sphere. The theoretical framework is based on theories by Connell and Bourdieu. The result shows that doping can be understood in relation to a narrative about being something out o the ordinary, but also that the anti-doping interventions lack a path of clear direction.

Doping als psychosoziales Verhalten stellt mehr als die bloße Verletzung von Anti-Doping-Regeln dar. Doping betrifft sowohl den (Hoch-)Leistungs- als auch den Breiten- und Freizeitsport. Für die Vorhersage von Doping haben Einstellungen einen nachweislich großen Einfluss, jedoch konnte bisher kein zufriedenstellendes theoretisches Modell, welches sowohl Personen- und Situationsfaktoren ausreichend berücksichtigt, gefunden werden. Die erste Publikation konzentriert sich auf methodische Fragen zur Erfassung impliziter Dopingeinstellungen. Dieser Beitrag, der auf duale Einstellungstheorien (z.B., Fazio, 1990) basiert, diskutiert die indirekte Messung von Dopingeinstellungen mittels Impliziten Assoziationstest (Greenwald et al., 1998). Die zweite Publikation stützt sich auf ein erweitertes Dopingverständnis und untersucht den Schmerzmittelkonsum in Abhängigkeit von Situations- und Personenfaktoren anhand der Annahmen von Rational-Choice-Theorie und Routine-Activity-Ansatz (Clarke & Cornish, 1985; Cohen & Felson, 1979). Abschließend wird der Einfluss von moralischen Entscheidungen auf Doping diskutiert (z. B. Ntoumanis et al., 2014). Das Ethische Dilemmatraining (Elbe & Brand, 2016) stellt eine neue Methode dar, um die moralische Entscheidungsfähigkeit zu steigern und moralische Werte und Überzeugungen zu stärken. Als integrierender theoretischer Rahmen wird die Situational Action Theory (z. B., Wikström, 2014) vorgeschlagen, welche sowohl die Interaktion von Personen- und Situationsfaktoren als auch die moralische Entscheidungsfindung miteinander verbindet. Weiterführende Implikationen für die Dopingforschung und -prävention werden diskutiert.:List of Publications III List of Figures IV List of Tables VI Abstract VII Zusammenfassung VIII 1 Introduction 1 1.1 Historical Overview on Doping in Sports 1 1.2 Defining Doping - Strengths and Weaknesses 3 1.2.1 World Anti-Doping Code 4 1.2.2 Limitations of WADA Code 6 1.2.3 Doping as (Deviant) Social Behavior 7 2 A Review on Current Research on Doping 11 2.1 Prevalence of Doping Elite and Recreational Sport 11 2.2 Doping and Legal and Illegal Substance (Mis-)Use 15 2.3 Doping and Related Personal and Situational Factors 19 3 Modelling Doping - A Theoretical Overview 21 3.1 A Summary of Relevant Theories on Doping 21 3.1.1 Theory of Planned Behavior 21 3.1.2 Sport Drug Control Model 22 3.1.3 Life Cycle Model 24 3.1.4 Trans-contextual Model of Avoid Doping 25 3.2 A Dual-process Perspective on Doping 27 3.3 A Person-Situation Perspective - Impact of Situation and Context 31 3.4 A Gateway to Doping 34 3.5 Implication for Further Theoretical Discussion 37 4 Aim of Thesis and General Research Questions 39 5 Publications 43 5.1 Publication Manuscript 1 43 5.2 Publication Manuscript 2 51 5.3 Publication Manuscript 3 61 6 General Discussion and Conclusion 79 6.1 Future Perspectives on Research of Doping Attitudes 79 6.2 A Different Understanding of the Person-Situation Interaction 81 6.3 Bridging the Gap - An Integrative Model of Person and Situation 82 6.3.1 Situational Action Theory (SAT) 82 6.3.2 SAT - New Implications for Doping Research 90 6.4 Future Implications and Challenges for Doping Prevention 95 6.5 Conclusion 97 7 References 101 8 Appendix 130 8.1 Thesis Statement of Originality 130 8.2 Statement of Authorship 131 8.3 Curriculum Vitae 132 / Doping - as a psychosocial behavior - is more than simply violating anti-doping rules and laws. Doping has become a part of high-performance and recreational sports. Attitudes toward doping among athletes have been sufficiently proven as an important impact factor. However, theoretical consideration of the person-situation mode with other relevant factors (e.g., moral disengagement or situational factors) into an integrated understanding of doping is still needed. The first study presented in this thesis focusses on methodological issues and the assessment of implicit doping attitudes. Based on dual process theories of attitudes (e.g., Fazio, 1990), the first paper discusses the indirect measurement of doping attitudes via the Implicit Association Test (Greenwald et al., 1998) as a tool to overcome limitations of self-reported attitudes. The second study presented here uses a broader understanding of doping and focuses on the parallelism of doping and doping-like behavior. The study analyzes the use of NSAID (non-steroidal anti-inflammatory drugs) in different intake situations. The influence of situation and person factors is tested by using the assumption of high- and low-cost situations from the Rational Choice Theory and Routine Activity Approach (e.g., Clarke & Cornish, 1985; Cohen & Felson, 1979). Finally, research has shown that moral decision-making has an impact on doping behavior (e.g., Ntoumanis et al., 2014). Ethical dilemma training (e.g., Elbe & Brand, 2016) may be employed as a new method to increase moral decision-making ability and to strengthen moral values and beliefs. Accordingly, the third research paper presented discusses this as a new approach to doping prevention. Overall, this thesis introduces Situational Action Theory ( e.g., Wikström, 2014) as an integrative theoretical approach, focussing on the person-situation interaction, as well as on moral decision-making. Further implications for doping research and for prevention are discussed.:List of Publications III List of Figures IV List of Tables VI Abstract VII Zusammenfassung VIII 1 Introduction 1 1.1 Historical Overview on Doping in Sports 1 1.2 Defining Doping - Strengths and Weaknesses 3 1.2.1 World Anti-Doping Code 4 1.2.2 Limitations of WADA Code 6 1.2.3 Doping as (Deviant) Social Behavior 7 2 A Review on Current Research on Doping 11 2.1 Prevalence of Doping Elite and Recreational Sport 11 2.2 Doping and Legal and Illegal Substance (Mis-)Use 15 2.3 Doping and Related Personal and Situational Factors 19 3 Modelling Doping - A Theoretical Overview 21 3.1 A Summary of Relevant Theories on Doping 21 3.1.1 Theory of Planned Behavior 21 3.1.2 Sport Drug Control Model 22 3.1.3 Life Cycle Model 24 3.1.4 Trans-contextual Model of Avoid Doping 25 3.2 A Dual-process Perspective on Doping 27 3.3 A Person-Situation Perspective - Impact of Situation and Context 31 3.4 A Gateway to Doping 34 3.5 Implication for Further Theoretical Discussion 37 4 Aim of Thesis and General Research Questions 39 5 Publications 43 5.1 Publication Manuscript 1 43 5.2 Publication Manuscript 2 51 5.3 Publication Manuscript 3 61 6 General Discussion and Conclusion 79 6.1 Future Perspectives on Research of Doping Attitudes 79 6.2 A Different Understanding of the Person-Situation Interaction 81 6.3 Bridging the Gap - An Integrative Model of Person and Situation 82 6.3.1 Situational Action Theory (SAT) 82 6.3.2 SAT - New Implications for Doping Research 90 6.4 Future Implications and Challenges for Doping Prevention 95 6.5 Conclusion 97 7 References 101 8 Appendix 130 8.1 Thesis Statement of Originality 130 8.2 Statement of Authorship 131 8.3 Curriculum Vitae 132