Made available in DSpace on 2014-12-17T15:18:10Z (GMT). No. of bitstreams: 1 MariaAGD.pdf: 3831855 bytes, checksum: 0abe0a10e359f22bab7ed834fa8e296a (MD5) Previous issue date: 2009-02-26 / Sugarcane is one of the most important products of the world and Brazil is responsible for 25 % of the world production. One problem of this culture at northeast of Brazil is the early flowering. In our laboratory, it has been made before four subtractive libraries using early and late flowering genotypes in order to identify messages related to the flowering process. In this work, two cDNAs were chosen to make in silico analysis and overexpression constructs. Another approach to understand the flowering process in sugarcane was to use proteomic tools. First, the protocol for protein extraction using apical meristem was set up. After that, these proteins were separated on two bidimensional gels. It was possible to observe some difference for some regions of these gels as well as some proteins that can be found in all conditions. The next step, spots will be isolated and sequence on MS spectrometry in order to understand this physiological process in sugarcane / A cana-de-a??car ? uma das mais importantes culturas mundiais e atualmente o Brasil representa um dos maiores produtores de cana-de-a??car no ranque mundial. Sabendo-se da import?ncia da cana-de-a??car nos dias atuais, principalmente em rela??o ao biocombust?vel e do problema causado pela flora??o precoce a esta cultura na regi?o Nordeste, foi realizada uma an?lise in silico de dois cDNAs:, 14-3-3 like protein e Protein kinase C inhibitor-like (PKCI), envolvidos no processo de flora??o da cana-de-a??car, utilizando ferramentas gen?micas. Foi escolhido o cDNA PKCI para a constru??o de cassetes de super-express?o de modo a ser caracterizado o papel deste cDNA no processo de flora??o. Outra abordagem utilizada nesse trabalho foi de analisar prote?nas totais de ?pices meristem?ticos de variedades precoce e tardia em g?is uni e bidimensionais. Os resultados mostraram que existem algumas prote?nas que podem ser caracter?sticas de uma das variedades, e em outras foi observado uma express?o diferencial

Made available in DSpace on 2014-12-17T15:18:12Z (GMT). No. of bitstreams: 1 CristianeMF.pdf: 69057 bytes, checksum: 86b55b192a6c5915d66e542325da022c (MD5) Previous issue date: 2007-02-28 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The northeastern region is responsible to 14.32% of sugarcane national production. This lowered contribution is due to edaphoclimatic condition. Flowering is a vital process to plant which consumes lots of energy and it culminates in a process called isoporization. This one can give in a decreasing of 60% on alcohol and water production. It may consider that cropped sugarcane has a hibrid with octaploid genome, there are varieties with a flowering standard until of non flowering. Using this natural genetic potential on different croppings of sugarcane, the aim of this work was to understand as this process occurs by the usage of subtractive approaches. The total RNA was extracted using Trizol of peaks of merisematics of croppings with induced flowering and other with late flowering. From this total RNA were built four subtractives libraries (B1- induced early flowering subtracted on late flowering not induced; B2- late flowering not induced subtracted induced early flowering; B3- induced early flowering subtracted of not induced early flowering; B02- not induced early flowering subtracted from induced early flowering) using kits Super Smart cDNA synthesis and BD Clontech kit select cDNA subtraction (Clontech). This material was clone don vector pGEM T-easy(Promega) and changed in competent cells of E.coli DH10B. Given analysis sequence was carried out a program BLASTn against database of NCBI and genome of Arabidopsis thaliana, rice and maize. Clones were grouped in 9 different classes according to function. Some factors already related as couples of flower induction were identified at different libraries. And grouped proteins with cell cycle and it controls were presents, mainly kinases proteins. Related factors to proteic sinthesis, metabolism, defence, cell communication were also given in both libraries .Some identified genes did not show similarity on database or homology with hypothesis function, and it can represents new genes to be deposited in international database. These results offers that some identified on sugarcane, classified as on factors classes, cell cycle and cell communication, trough unknown genes, can be linked with genetic changing to the flowering process found in the northeastern region / A regi?o nordeste ? respons?vel por 14,32% da produ??o nacional de cana-de-a??car. Esta contribui??o reduzida ? devido ?s condi??es edafo-clim?ticas da regi?o. A flora??o ? um processo vital para a planta que consome muita energia e culmina num fen?meno denominado de isoporiza??o do colmo. Esta isoporiza??o pode acarretar numa redu??o de at? 60% na produ??o de ?lcool e a??car. Considerando que a cana-de-a??car cultivada corresponde a um h?brido com o genoma octaploide, existem variedades com um padr?o de florescimento precoce at? um padr?o de n?o florescimento. Utilizando este potencial gen?tico natural presente nas diferentes cultivares de cana-de-a??car na regi?o nordeste, o objetivo do presente trabalho foi de compreender como ocorre esse processo de flora??o por meio da metodologia de bibliotecas subtrativas. O RNA total foi extra?do utilizando o reagente Trizol (Invitrogen) de ?pices meristem?ticos de cultivares com florescimento precoce induzida e n?o induzida e outra com florescimento tardio. A partir deste RNA total foram constru?das as quatro bibliotecas subtrativas (B1 florescimento precoce induzida subtra?da da tardia n?o-induzida; B2 florescimento tardio n?o-induzida subtra?do da precoce induzida; B3 precoce induzida subtra?da da precoce n?o-induzida; B02 precoce n?o-induzida subtra?da da precoce induzida), utilizando os kits Super Smart cDNA syntesis e o kit BD Clontech PCR select cDNA subtraction (Clontech). Este material foi clonado no vetor pGEM T-easy (Promega) e transformados em c?lulas competentes de E. coli DH10B. A an?lise das seq??ncias obtidas foi realizada utilizando o programa BLASTn contra o banco de dados do NCBI e do genoma de Arabidopsis thaliana, arroz e milho. Os clones foram agrupados em 9 diferentes classes de acordo com a fun??o. Alguns fatores de transcri??o j? relatados como participantes da via de indu??o floral foram identificados nas diferentes bibliotecas. Al?m disso, prote?nas associadas com o ciclo celular e seu controle tamb?m estavam presentes, destacando-se nesse grupo as prote?nas quinases. Fatores relacionados ? s?ntese prot?ica, metabolismo, defesa, transporte e comunica??o celular, tamb?m foram obtidos em ambas bibliotecas. Alguns genes identificados nas bibliotecas n?o apresentaram similaridade nos bancos de dados ou apresentaram homologia com genes de fun??o hipot?tica, e podem representar novos genes a serem depositados em bancos de dados internacionais. Estes resultados sugerem que alguns dos genes identificados em cana-de-a??car, classificados tanto na classe de fatores de transcri??o, ciclo celular e comunica??o celular, al?m dos genes n?o conhecidos, possam estar associados com a variabilidade gen?tica para o processo de flora??o encontrada na regi?o nordeste

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-03-21T18:51:09Z No. of bitstreams: 1 MychelleKytchiaRodriguesNunesDuarte_DISSERT.pdf: 2556724 bytes, checksum: 7d5fee1d4fe61eba6aa3c5ddd4fa6747 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-03-28T00:32:58Z (GMT) No. of bitstreams: 1 MychelleKytchiaRodriguesNunesDuarte_DISSERT.pdf: 2556724 bytes, checksum: 7d5fee1d4fe61eba6aa3c5ddd4fa6747 (MD5) / Made available in DSpace on 2017-03-28T00:32:58Z (GMT). No. of bitstreams: 1 MychelleKytchiaRodriguesNunesDuarte_DISSERT.pdf: 2556724 bytes, checksum: 7d5fee1d4fe61eba6aa3c5ddd4fa6747 (MD5) Previous issue date: 2016-12-06 / A doen?a arterial coronarina ? uma das principais causa de ?bitos em todo o mundo e pode ser desencadeada por altera??es no perfil s?rico de ?cidos graxos, nos padr?es de express?o g?nica, no consumo alimentar e diet?tico e por desequil?brios antropom?tricos e/ou de composi??o corporal. O diagn?stico precoce desta doen?a ? um desafio na medicina translacional, visto que os m?todos utilizados s?o onerosos e/ou invasivos. O trabalho ent?o teve como objetivo identificar biomarcadores para o diagn?stico precoce da aterosclerose por meio do perfil serico de ?cidos graxos, express?o do RNAm da enoil-coA-hidratase (ECDHC3) e de fatores nutricionais em pacientes sem les?es e com diferentes extens?es da les?o ateroscler?tica. Realizou-se um estudo observacional, com a amostragem coletada por conveni?ncia, formada por pacientes que iriam realizar pela primeira vez a cinecoronariogradia no setor de Hemodin?mica do Hospital Universit?rio Onofre Lopes. Na primeira casu?stica participam 59 pacientes (n1, 2011-2013) e na segunda 41 (n2, 2014-2015), ambos com idade entre 30-74 anos. Determinou-se a extens?o das les?es por meio do ?ndice de Friesinger, o perfil bioqu?mico (glic?mico,lipid?mico) por espectrometria semiautomatizado; concentra??o s?rica de ?cidos graxos pela cromatografia gasosa; express?o do RNAm do ECHDC3 pela rea??o de cadeia de prolimerase (PCR) em tempo real; consumo alimentar e diet?tico por dois recordat?rios de 24h, par?metros antropom?tricos e composi??o corporal pela balan?a com bioimped?ncia el?trica, fita m?trica e estadiometro. Os pacientes foram classificados em grupos: sem les?o (n1=18/n2 =8), poucas les?es (n1=17/n2=6), intermedi?rias (n1=17/n2=15) e graves (n1=7/n2=12). Na primeira casu?stica foi encontrado elevadas concentra??es s?ricas de ?cido ol?ico e de ?cidos graxos monoinsaturados nos pacientes com poucas les?es e intermedi?rias, quando comparado com pacientes sem les?o (p<0,05). A express?o do ECHDC3 foi 1,2 vezes mais alta em pacientes com poucas les?es que em pacientes sem les?o (p=0,023), e 1,8 vezes mais baixa em pacientes com les?o grave que em pacientes sem les?o (p=0,020). Na segunda casu?stica, a express?o do ECHDC3 foi 1,93 mais alta em pacientes com les?o intermedi?ria do que com poucas les?es (p = 0.011) e 1,91 vezes mais alta em pacientes com les?o grave que em poucas les?es (p = 0.013). A an?lise de Spearman mostrou uma correla??o entre os grupos de Friesinger com a express?o do ECHDC3 (r = 0.327, p =0.037) , e deste com o percentual de gordura visceral (r = 0.416, p =0.009). O ?ndice de Friesinger tamb?m mostrou uma correla??o com o consumo de carboidrato (r = 0.754, p = 0.002), vitamina B1 (r = 0.507, p = 0.001), vitamina B2 (r = 0.388, p = 0.012), folato (r = 0.599, p = 0.000) e magn?sio (r = 0.528, p = 0.003). Verificou-se que o aumento de ?cido ol?ico s?rico, da express?o do ECHDC3 e da gordura visceral podem contribuir para a progress?o da aterosclerose, uma vez que estes podem favorecer a ? ? oxida??o, rea??o que favorece a forma??o de esp?cies reativas de oxig?nio, desencadeando inflama??o, um dos principais gatilhos para a aterosclerose. Tamb?m foi visto que o consumo de carboidratos e alguns micronutrientes podem favorecer a progress?o da aterosclerose, j? que o aumento da ingest?o destes nutrientes pode propiciar a defici?ncia na absor??o ou estresse oxidativo, que implica diretamente na etiologia desta doen?a. / Coronary arterial disease is a at leading cause of death worldwide and can be triggered by alterations in the serum profile of fatty acids, by patterns of gene expression, changes in dietary intake, and alteration anthropometric. The early diagnosis of this pathophysiology is a challenge in translational medicine, since the methods used are expensive and / or invasive. The aim in the study was to identify biomarkers for the early diagnosis of atherosclerosis through the relationship of serum fatty acid profile, enoyl-coA hydratase (ECDHC3) mRNA expression and nutritional factors in patients without lesions and with different extensions of the atherosclerotic lesion. The study was observational, with the sample collected for convenience in two casuistry in the Hemodynamics sector of the Onofre Lopes University Hospital (HUOL). In the first series, 59 patients partipated (n1, 2011-2013) and in the second 41 individuals ( n2, 2014-2015), both aged 30-74 years and who were undergoing coronariography for the first time. The extent of atherosclerotic lesions was determined by Friesinger index, the biochemical profile by semi-automated spectrometry; the concentration of fatty acids by gas chromatography; gene expression of ECHDC3 mRNA by real-time PCR; food consumption and dietary intake for two 24-hour dietary intake recalls, anthropometric parameters and corporal composition by the electric bioimpedance, measuring tape and stadiometer scale.Patients were classified into groups: no lesion (n1 = 18 / n2 = 8), low lesions (n1 = 17 / n2 = 6), intermediate lesions (n1 = 17 / n2 = 15) and major lesion (n1= 7 / n2 = 12). In n1 was observed high serum concentrations of oleic acid and monounsaturated fatty acids in patients with low lesions and intermediate when compared to patients without lesions (p <0.05). ECHDC3 expression was 1.2 fold-higher in patients with low lesions than in patients without lesions (p = 0.023), and 1.8 fold- lower in patients with major lesions than in patients without lesions (p = 0.020). In the second casuistry, the expression of ECHDC3 was 1.93 fold-higher in patients with intermediate lesions than in low lesions (p = 0.011) and 1.91 fold-higher in patients with severe lesions than in low lesions (p = 0.013). Spearman's analysis showed a positive correlation showed a between ECHDC3 mRNA expression and Friesinger index (r = 0.327, p = 0.037), between the gene and visceral fat (r = 0.416, p = 0.009). The Friesinger index also showed a correlation with the consumption of carbohydrate (r = 0.754, p = 0.002), vitamin B1 (r = 0.507, p = 0.001), vitamin B2 (r = 0.388, p = 0.012), folate (r = 0.599, p = 0.000), magnesium (r = 0.528, p = 0.003). It was verified that oleic acid, ECHDC3 expression, percentage of visceral fat could favors ?-oxidation, trigger for inflammation and CVD; while the greater consumption of carbohydrate and some micronutrients, also could be associated with the CVD development, since the excess or decreased absorption can favors the oxidative stress, that favors the atherosclerosis.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-10-04T23:54:46Z No. of bitstreams: 1 CarolinaAraujoSousa_DISSERT.pdf: 4069494 bytes, checksum: 9b768a46fbe08e2407ed147faed6bc74 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-10-16T20:38:12Z (GMT) No. of bitstreams: 1 CarolinaAraujoSousa_DISSERT.pdf: 4069494 bytes, checksum: 9b768a46fbe08e2407ed147faed6bc74 (MD5) / Made available in DSpace on 2017-10-16T20:38:12Z (GMT). No. of bitstreams: 1 CarolinaAraujoSousa_DISSERT.pdf: 4069494 bytes, checksum: 9b768a46fbe08e2407ed147faed6bc74 (MD5) Previous issue date: 2017-08-31 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / A forma??o de circuitos neurais durante o desenvolvimento se d? atrav?s de uma complexa intera??o entre fatores gen?ticos e ambientais, que influenciam m?ltiplos eventos como a neurog?nese, a sinaptog?nese e a mieliniza??o. Em transtornos do desenvolvimento, como o transtorno do espectro autista (TEA), intercorr?ncias nesse processo levam ? m?-forma??o da circuitaria neural e, consequentemente, a d?ficits de intera??o social, interesses restritos e movimentos estereotipados, entre outros. Recentemente, realizamos em nosso laborat?rio a an?lise do transcriptoma do c?rtex frontal no modelo animal de autismo induzido por exposi??o a ?cido valpr?ico (VPA) in utero. Observamos que ratos com 15 dias de idade (P15) apresentam aumento da express?o de genes relacionados ? estabilidade sin?ptica e redu??o de genes relacionado ? mielina, sugerindo poss?veis mecanismos moleculares para as varia??es comportamentais previamente observadas nestes animais. Portanto, o objetivo desta disserta??o foi aprofundar este estudo investigando o padr?o de mieliniza??o no enc?falo de animais tratados com VPA em diferentes idades p?s-natais (infantil: P15 e adulta: P60). Para tanto, os grupos experimental e controle foram gerados, respectivamente, atrav?s da inje??o de VPA (500 mg/kg i.p.) ou salina em f?meas gr?vidas durante o dia embrion?rio 12.5 (E12.5). A an?lise da integridade da mielina foi realizada por duas abordagens: (1) an?lise da express?o de genes relacionados ? mielina (Mobp, Plp1, Mag e Klhl1), por PCR quantitativo, no c?rtex frontal de animais em P15; e (2) quantifica??o histol?gica da distribui??o de mielina em cinco sub-regi?es do c?rtex frontal e corpo caloso de animais P15 e P60. Dos quatro genes avaliados, observamos significativa diminui??o na express?o de Mobp e Mag em animais VPA em P15. A an?lise histol?gica de mielina mostrou redu??o significativa na intensidade de marca??o no c?rtex cingulado anterior em animais VPA em P60, por?m n?o detectou diferen?a na intensidade de marca??o em animais VPA em P15 quando comparados aos controles. Conclu?mos assim que animais VPA neonatos t?m reduzida express?o de genes relacionados a compacta??o da mielina, por?m sem altera??es no conte?do lip?dico da mielina nas ?reas analisadas. Animais adultos, por sua vez, apresentaram altera??es no conte?do lip?dico da mielina no c?rtex cingulado anterior. Em conjunto, estes resultados sugerem que dist?rbios na comunica??o entre circuitos frontais neste modelo de autismo podem ocorrer inicialmente devido altera??es na organiza??o da mielina, levando a redu??es de mielina no adulto. / The formation of brain circuits during neural development occurs through the interaction between timely regulated genetic and environmental signals, which influence multiple events such as neurogenesis, synaptogenesis, and myelination. In developmental disorders, such as autism spectrum disorders (ASD), deficits in these processes may lead to neural circuitry malformations and, in consequence, to social interaction deficits, restricted interests, and stereotyped movements, among others. Recently, we performed a transcriptome analysis of the frontal cortex of an animal model of autism induced by valproic acid (VPA) in utero in our lab. We observed that VPA animals at the postnatal age 15 (P15) show an increased expression of genes related to synaptic stability and a decrease in the expression of myelin-related genes, suggesting possible molecular mechanisms for the behavioral deficits previously observed in these animals. Therefore, the aim of this Master?s thesis was to further investigate the myelination pattern in the forebrain of VPA-treated rats at different postnatal ages (infant: P15 and adult: P60). For that, experimental and control groups were generated by injecting pregnant dams with 500 mg/Kg i.p. VPA or saline, respectively, on embryonic day 12.5 (E12.5). Analysis of myelin integrity was conducted by two different approaches: (1) gene expression analysis of myelin-related genes (Mobp, Plp1, Mag, and Klhl1) in dissected samples of the frontal cortex of P15 rats by quantitative real-time PCR; and (2) histological quantification of myelin distribution in five sub-regions of the frontal cortex and corpus callosum of P15 and P60 animals. Of all genes analyzed, we observed a significant decrease in Mobp and Mag expression in P15 VPA animals. Myelin analysis showed a significant reduction in myelin staining in the anterior cingulate cortex of VPA animals at P60, but no differences were observed at P15. In conclusion, infant VPA rats showed reduced expression of genes related to myelin assembly, without alterations in the lipid content of myelin in the areas analyzed. Adult animals, in contrast, showed a decrease in lipid content of myelin in the anterior cingulate cortex. Together, these results suggest that communication abnormalities in frontal circuits in this animal model of autism may occur initially due to alterations in myelin organization, leading to myelin reduction in adulthood.

This study is engaged in express emergency methods to monitor internal contamination of people in the event of the radiological emergency.

Made available in DSpace on 2016-04-26T19:39:32Z (GMT). No. of bitstreams: 1 2009 - Leandro Azevedo Santos.pdf: 3069700 bytes, checksum: dbd1726a5b683e7f290e1829143eacac (MD5) Previous issue date: 2009-06-03 / Funda??o Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro / To improve nitrogen usage efficiency in plants the rice transcriptional factor OsDof25 was identified and cloned, whose probably orthologe is the maize ZmDof1, already identified and partially characterized. The ZmDof1 was also cloned for comparative analysis with OsDof25, in order to confirm this last one as ZmDof1 orthologe in rice. The constructions for Arabidopsis superexpression of these transcriptional factors were made using the cloning system of gateway technology (Invitrogen), to obtain the expression vectors 35S:ZmDof1:HA and 35S:OsDof25:HA. Lineages with different expression levels of these genes were obtained, but with only one inserted copy. These transgenic lineages when grown in a half strength of MS medium (10mM of NH4 + and 20mM of NO3 -) showed phenotypes with chloroses and growth difficulty; although when they were cultured in soil they showed great vegetative development and delay in the inflorescence emission. When analyzed the gene expression changes induced by the superexpression of these transcriptional factors, it was observed that both genes produced an increase in the expression levels of high and low affinity ammonium transporters (AMT1.1 and AMT2.1, respectively), indicating that these phenotypes may be due to the toxic effect of an excess of ammonium uptake. We also verified an increase of expression for pyruvate kinase (PK1 and PK2), and phosphoenolpyruvate carboxylase (PEPC1 and PEPC2). Pyruvate kinase converts phophoenolpyruvate (PEP) to pyruvate, and phosphoenolpyruvate carboxylase converts PEP to oxalacetate, which is substrate for malate dehydrogenase to form malate. Both pyruvate and malate may feed the Krebs cycle. In addition, there was an increase in the expression of isocitrate dehydrogenase, which is present in the citosol and mitochondria, needed for converting isocitrate to 2- oxoglutarate. Thus, it was hypothesized that the increase of expression levels of these carbon metabolism enzymes was necessary to increase the production of 2-oxoglutarate and, consequently, to reduce the toxic effect of ammonium uptaked. Besides, it was observed an increase of expression levels and activity of glutamate dehydrogenase (GDH). This enzime may work as much in the direction of glutamate amination as in deamination, when the plants were submitted to ammonium excess or carbon limitation conditions, respectively. / Com o objetivo de aumentar a efici?ncia de uso de nitrog?nio (EUN) em plantas, foi identificado e clonado o fator de transcri??o OsDof25 de arroz, cujo prov?vel ort?logo ? o ZmDof1 de milho, j? identificado e parcialmente caracterizado. Tamb?m foi clonado o ZmDof1 para an?lises comparativas com o OsDof25, a fim de comprovar que este ?ltimo ? realmente ort?logo do ZmDof1. As constru??es para superexpress?o destes fatores de transcri??o em Arabidopis foram feitas utilizando o sistema gateway de clonagem para obten??o dos vetores de express?o 35S:ZmDof1:HA e 35S:OsDof25:HA. Foram obtidas linhagens com diferentes n?veis de express?o destes genes, mas com apenas uma inser??o. As linhagens transg?nicas obtidas quando crescidas em meio MS ? for?a i?nica (10mM de NH4 + e 20mM de NO3 -) apresentaram fen?tipos como clorose e dificuldade de desenvolvimento, ao passo que quando cultivadas em solo mostraram desenvolvimento vegetativo mais intenso e atraso para emiss?o da infloresc?ncia. Quando analisadas as modifica??es de express?o g?nica causadas pela superexpress?o destes fatores de transcri??o, observou-se que ambos os fatores de transcri??o provocaram aumento de express?o dos transportadores de am?nio de alta e baixa afinidades (AMT1.1 e AMT2.1 respectivamente), indicando que o fen?tipo observado pode ser devido ao efeito t?xico do excesso de am?nio absorvido. Verificou-se tamb?m aumento de express?o das enzimas piruvato quinase (PK1 e PK2) e fosfoenolpiruvato carboxilase (PEPC1 e PEPC2). A piruvato quinase converte o fosfoenolpurato (PEP) a piruvato, enquanto a fosfoenolpiruvato carboxilase converte o PEP a oxalacetato (OAA) que pode sofrer a??o da malato desidrogenase originando o malato. Ambos os metab?litos, piruvato e malato, alimentam o ciclo de Krebs. Houve tamb?m aumento de express?o da isocitrato desidrogenase, enzima presente na mitoc?ndria (ciclo de Krebs) e no citosol que converte isocitrato a 2-oxoglutarato (2-OG). Assim, ? prov?vel que o aumento da express?o destas enzimas do metabolismo de carbono foi necess?rio para aumentar a produ??o de 2-OG e, por conseguinte, diminuir o efeito t?xico do excesso de am?nio absorvido. Al?m disso, observou-se aumento de express?o e atividade da glutamato desidrogenase (GDH). Essa enzima pode atuar tanto na dire??o da amina??o, quanto na dire??o da desamina??o, em condi??es de excesso de am?nio e/ou sob condi??es de limita??o de carbono nas plantas, respectivamente.

Rapporten presenterar skapandet av en produktkonfigurator och flera förslag på hur den kan förbättras. Uppdraget gavs av System Andersson till två studenter på Jönköpings Tekniska Högskola som genomförde detta som sitt examensarbete. Rapporten diskuterar möjliga lösningar på en produktkonfigurator och visar ett resultat som utgår från dessa lösningar. Produktkonfiguratorn i rapporten består av två delar. Dels konfigurationsdelen som hjälper användaren att skapa nya produkter utifrån valda artiklar. Artiklarna sorteras automatiskt av produktkonfiguratorn så att endast kompatibla artiklar kan sättas ihop. Detta sker med ett ”drag and drop” gränssnitt. Dessutom innehåller produktkonfiguratorn en kompatibilitetsdel, vars uppgift är att underlätta vid definierandet av kompatibilitet mellan artiklar. Detta kan göras löpande under tiden en produkt konfigureras om så önskas. Användaren kan välja på två eller flera artiklar som ska vara kompatibla med varandra, eller hela kategorier. Sist i rapporten diskuteras de val som gjorts under arbetets gång och möjliga förbättringar som kan göras på produktkonfiguratorn.

Road transport is most commonly used out of other modes in terms of freight and passenger transportation on local and regional levels. For European citizens it is a primary mean of their access to services, social activities and employment. The scope of road infrastructure differs in some regions. The major disparity appears when the East of Europe is compared with the West. Actions to link periphery with the core of Europe are being continuously performed, what in this case represents itself in constructing Pan-European Transport Corridors, which will contribute to the connection of the whole territory of Europe. The Via Baltica, chosen as a case for this thesis, is a route to connect Helsinki, Tallinn, Riga, Kaunas, Budzisko and Warsaw. It is the common initiative of Finland, Estonia, Lithuania, Latvia and Poland. In addition, it is to be an express road and part of the I Pan-European Transport Corridor, which is going to connect Baltic countries with the West and South part of Europe. We focus on presenting how the Via Baltica is introduced in official documents and compare it with the process in practice. Our final goal is to assess the planning process of the Via Baltica route in Poland according to top down planning approaches and sustainability criteria. We are trying to find out what are the advantages and disadvantages of the ongoing process. After introducing results of the analysis made for the Via Baltica by Polish experts, we present how stakeholders and local authorities try to reinforce the process, regardless of environmental issues, to achieve economic and social profits. As the result we try find out how are the dimensions of sustainable development handled in the Via Baltica case in Poland. The conclusions are based on our findings concerning several conflicts which appeared during the planning process. They are also based on one-dimension as well as multi-criteria analyses. / angelikakowalczyk@o2.pl monika.m.kustra@gmail.com

Cette étude aborde la manière dont l’Orient-Express a été décrit, perçu et imaginé, et s’efforce de mesurer l’écart entre les représentations communes et ce qui se dégage d’un corpus de textes très divers à travers trois grands axes de réflexion :les évocations des itinéraires de l’Orient- Express, les discours tenus sur le train et ses voyageurs, les thématisations et récupérations littéraires dont il a fait l’objet.L’idée directrice du propos est de montrer comment l’Orient-Express, rêve programmé par de très efficaces campagnes publicitaires et suscité par un imaginaire géographique, historique et littéraire puissant, n’a pu pleinement convaincre les voyageurs, mais aussi les écrivains et les lecteurs qui, semble-t-il, ne cessent de déplorer la disparition d’un train et d’un voyage qui n’ont peut-être jamais existé ou qui ne sont jamais vraiment ceux qu’ils imaginaient. / Doctorat en Langues, lettres et traductologie / info:eu-repo/semantics/nonPublished

Magister Legum - LLM