The master's thesis deals with promotional discounts of retail chains focusing on a description of factors that affect customers in selection of a shopping place to purchase food and beverages and identifies the level of influence of promotional discounts on purchase decisions. This thesis is divided into two parts. The theoretical part contains fundamental resources, that are enriched by findings of foreign case studies. It focuses on consumers behavior and purchase decisions emphasizing the influence of prices and special offers. The practical part consists of secondary research of current trends in shopping behavior of Czech consumers, primary analysis of the action flyers and research focused on describing the attitudes of consumers towards the retail chains and identifying the impact of promotional discounts on purchasing food products. The recommendations for marketing activities of retail chains are formulated based on the results of the analysis.

In thesis was verified micromethod for isolation of plant DNA from different vegetable (onion and broccoli) and plant food products in quality for application in polymerase chain reaction (PCR). The micromethod allows isolation DNA using magnetic particles from crude lysates of cells obtained by direct homogenization of plant tissues. Various methods of processing homogenates were compared. Homogenization was performed by lysis buffer containing cetyltrimethylammonium bromide (CTAB). The effect of the organic extraction agents was tested (chloroform-octanol and isopropanol). DNA was purified from homogenates by reversible adsorption on magnetic particles (four different types of magnetic particles were tested). The quality of isolated DNA was verified by UV spectrophotometry. The amplificabilty of DNA was tested by polymerase chain reaction (PCR). Specific primers for plant ribosomal DNA (rDNA) were used. PCR products of lenght 700 and 220 bp were detected by agarose gel electrophoresis. Differences in yield and quality of DNA were depended on the homogenate processing and magnetic particles used. The proposed procedure with two magnetic particles was tested for the isolation DNA from plan food products (spreads). DNA was amplified in PCR. Micromethod is suitable for DNA analysis of foods.

This paper examines the concept of sustainable development and sustainable consumption in the food industry and how consumers relate with the idea of organic food produce and their alternatives. The research was developed by using the Consumer Decision Making model and the Norm Activation Model to be able to understand what motivates consumers in their buying choices and why. These models break down the steps a consumer goes through before making a purchase and the emotional evaluation that occurs after that decision is made. With a total of 70 responses from a structured survey sent out, the results indicated that consumers are conscious about sustainable products, are aware of the environmental and social impact but 30.6% of consumers have never looked out for sustainable products.

In 2016, it is no longer possible to deny the existence of global warming and its harmful effects on our planet and the environment. Not only experts, but also consumers, have become increasingly concerned about the environment. This increased awareness and concern has increased consumers’ interest in purchasing environmentally friendly food products, and has caused companies to focus on programs intent on reducing their environmental impact and helping the environment. Companies have introduced more environmentally friendly food products, accessible on a large scale, and have changed their communication to focus more on green products and green product attributes. Scandinavian countries have some of the largest numbers of organic food consumers in Europe and the world, with the Swedish organic food industry measuring the highest growth rate in organic food sales in 2015. Therefore, this study focuses on the Swedish green food industry. A major way for companies to make consumers aware of their available environmentally friendly food products is through green advertising. An increasingly frequent problem, however, is that consumers tend to distrust these green ads. They have knowledge of green washing practices and misleading information in green advertising. In this thesis, the relationships between green consumerism, green washing and green advertising skepticism were investigated. The potential relationship between demographic factors such as gender, age, income and level and area of study, and green advertising skepticism were tested as well. A research model with three main hypotheses was used in this thesis. The results showed a significant relationship between green consumerism and green advertising skepticism when green washing was a moderator. There was also a significant negative relationship between attitude towards green products and green advertising skepticism and a positive relationship between environmental concern and green advertising skepticism. There existed no significant relationship between the tested demographic factors and green advertising skepticism in the Swedish green food industry.

Das Ziel der vorliegenden Studie ist, die Herkunftspräferenzen der slowakischen Verbraucher zu untersuchen und die Schlussfolgerungen und Empfehlungen für die marketingstrategischen und kommunikationspolitischen Maßnahmen zur Förderung der heimischen Produktion in der Slowakei zu konkretisieren. Der theoretische Abschnitt der Arbeit widmet sich den Prozessen des Verhaltens der Konsumenten aus psychologischer sowie marketingpolitischer Sicht und bietet eine ausführliche theoretische Bestandaufnahme zu den Themenbereichen Konsumentenverhalten, Image, Herkunft, Country-of-Origin. Darüber hinaus werden die Rahmenbedingungen für das Agrarmarketing und herkunftsbezogene Marketingmaßnahmen sowie die Ausgangssituation für das Marketing von Lebensmitteln der slowakischen Herkunft erläutert. Die Sekundäranalyse der Agrarmärkte Tschechiens, Polens und Ungarns bieten die Grundlage für die Verbesserung und neue Vorschläge zu den herkunftsbezogenen Marketingmaßnahmen in der Slowakei. Die empirische Forschung besteht aus 3 Teilen: Aus der standardisierten Konsumentenbefragung mittels Fragenbogens, aus Experteninterviews und aus dem Store/ Produkt-check. Im Fokus der schriftlichen Befragungen stehen die jungen slowakischen Konsumenten mit hohem Bildungsniveau, bei denen die Präferenz für die slowakischen Lebensmittel und deren Abhängigkeit von dem Image der Slowakei sowie die Einstellung zu den Ländern Deutschland, Tschechien und Polen untersucht werden. Die Experteninterviews bieten einen vertieften fachlichen Einblick auf das untersuchte Thema. Die Ergebnisse der Untersuchung offenbaren deutliche positive Einstellung zum Heimatland und positives Image der Slowakei. Dies lässt sich zum positiven Image der slowakischen Lebensmittel übertragen. Die kognitiven Kaufmotive spielen beim Kauf von slowakischen Lebensmitteln eine übergeordnete Rolle, wobei dem Herkunftskriterium eine gewisse Wichtigkeit zugeschrieben wird. Die erforschten Ergebnisse münden anschließend in die Erarbeitung von Hinweisen, Erweiterungs- und Verbesserungsvorschlägen des in der Slowakei angewendeten Förderungsprogramms für die einheimischen Lebensmittel. / This exploratory study examines the consumer perception and attitudes toward domestic food products among Slovakian consumers. Furthermore this gives recommendation for the improvement of marketing activities to promote domestic food products in Slovakia. The theoretical section of this work is dedicated to the investigation of the processes of consumer behaviour from a psychological and marketing point of view. This section explores and offers a detailed theoretical explanation to the following topics: consumer behaviour, image, origin, country-of-origin. In addition, the framework of agricultural marketing and marketing activities based on origin of the products as well as the situation for the marketing of food products with Slovakian origin are explained. The secondary analyses of the agricultural markets in Czech republic, Poland and Hungary are the basis for the improvement and new suggestions for the marketing activities for the food products in Slovakia. The empirical research consists of 3 parts: consumer standardised surveys, interviews with experts and methods of store-check/ product-check. The consumer surveys focus on young Slovakian consumers with higher education level and their attitudes and preference toward domestic food products and check if they are dependent on portraying the image and assosiations with their home-country. The surveys explor the attitudes toward foreign food products from Germany, Czech republic and Poland as well the country-image of these countries. Interviews with experts provide deeper academic and professional exploration to the investigated field. The results of the scientific surveys reveal significant positive attitudes to the home- country and positive image of Slovakia among the chosen group of Slovakian consumers. This has an influence on the positive image of Slovakian food products. The cognitive buying motives play a significant role in the purchase of Slovakian food products, however the country-of-origin criteria demonstrates an importance as well. The results lead to the formulation of references, improvement recommendations and suggestions for the marketing program for domestic food products in Slovakia.

Dans le secteur de l'agroalimentaire, l'utilisation de la RFID permet d'améliorer la sécurité des produits alimentaires tout en assurant une meilleure traçabilité et un meilleur suivi des produits. Le travail de cette thèse était principalement réalisé dans le cadre d’un projet en coopération avec plusieurs partenaires académiques et industriels dans le secteur fromager où l’objectif principal du projet était de maitriser l’ensemble des étapes de fabrication par un parfait suivi de la traçabilité des produits et un pilotage précis de l’affinage. Le travail réalisé dans cette thèse s'inscrit dans ce contexte et a comme objectif le développement de tags RFID UHF adaptés aux contraintes des produits alimentaires en assurant une double fonction: une fonction de traçabilité à identification unitaire, et ensuite une fonction de détection du degré de maturation de l'aliment par mesure indirecte de ses propriétés électromagnétiques et physico-chimiques.La première étape du projet était la caractérisation des paramètres électromagnétiques des produits alimentaires durant l’affinage afin de réaliser une conception appropriée de l'antenne du tag. En radiofréquences, c'est principalement le substrat qui dicte les propriétés électromagnétiques des composants, notamment la taille des antennes et leur diagramme de rayonnement. La connaissance de ces propriétés est indispensable afin de répondre aux exigences industrielles et d'intégrer dans de bonnes conditions les systèmes dans l'environnement applicatif. En plus, une étude de corrélation est réalisée entre la variation des propriétés diélectriques et celle des propriétés chimiques durant la période d’affinage des produits. Cette étude aide à comprendre la différence entre les différents produits et permet de déterminer les paramètres principaux à suivre durant l’affinage pour la conception du tag capteur.La deuxième étape était la conception des tags identifiants pour la traçabilité des produits alimentaires durant la procédure de fabrication. L’objectif principal est de réaliser un tag identifiant avec une performance qui répond aux besoins des fabricants dans le secteur fromager concernant la taille, la mémoire et la distance de lecture du tag tout en respectant les normes de santé au niveau d’emballage du tag ainsi que le substrat utilisé. Plusieurs configurations de tag RFID UHF étaient réalisées et testées dans un environnement industriel où les résultats obtenus montrent l’efficacité d’utiliser un système de RFID pour automatiser la traçabilité des produits dans le secteur fromager.La troisième étape concerne la conception des tags capteurs pour suivre le degré d’affinage des produits pendant la période de maturation. Une première solution, basée sur l’exploitation de l’effet de variation des propriétés diélectriques sur la distance de lecture de tag, montre un manque de performance due aux faibles variations ainsi qu’aux difficultés de mesure dans un environnement réel. Une deuxième solution basée sur la mesure de taux de dégagement des gaz durant l’affinage montre la possibilité d’estimer le degré d’affinage. Par contre, la puissance d’activation de capteur et le coût élevé ne permettent pas d’adopter cette solution. Finalement, deux solutions alternatives étaient réalisées pour mesurer l’augmentation du taux d’échapement des gaz d’une manière indirecte. La première configuration de tag capteur est basée sur le suivi du changement de dimensions du produit en utilisant un capteur résistif. D’autre part, la deuxième configuration de tag capteur est basée sur le suivi de changement de pression dans l’emballage du produit dû à la production de gaz. Les résultats obtenus montrent que ces deux configurations de tag RFID capteur peuvent offrir des solutions simples et efficaces pour le pilotage de l’affinage des produits alimentaires. / In the food sector, the use of RFID makes it possible to improve the safety of food while ensuring better traceability and better monitoring of products. The work of the thesis was mainly carried out within the framework of a project in cooperation with several academic and industrial partners in the cheese sector where the main objective of the project is to develop UHF RFID tags adapted to the constraints of food products by ensuring a dual function: a traceability function with unitary identification, and then a sensing function of cheese maturation by indirect measurement of its electromagnetic and physicochemical properties.The first step of the project was the characterization of the electromagnetic parameters of food products during ripening in order to achieve an appropriate design of the tag antenna. Knowledge of these properties is essential to meet industrial requirements and to integrate systems in right conditions. Also, a correlation study is carried out between the variation of the dielectric properties and that of the chemical properties during the period of cheese ripening. This study helps to understand the difference between cheese types and allows determining the main parameters to follow during cheese maturation for the design of the sensor tag.The second step was the design of identification tags for the traceability of food products during the manufacturing process. The primary objective is to create a UHF RFID tag with a performance that meets the needs of manufacturers in the cheese sector regarding the size, memory and reading distance while respecting the health standards at the packaging level as well as the substrate used. Several UHF RFID tag configurations were realized and tested in an industrial environment where the results obtained show the effectiveness of using an RFID system to automate the traceability of products in the cheese sector.The third step is the design of sensor tags to track the degree of maturation of products during the ripening period. A first solution, based on the exploitation of the effect of variation of the dielectric properties on the tag reading distance, shows a lack of performance due to the small variations as well as the difficulties of measurement in a real environment. A second solution based on the analysis of gas evolution rate shows the possibility of estimating the degree of cheese maturation. However, the sensor activation power and the high cost represent significant challenges and thus it was difficult to adopt this solution. Finally, two alternative solutions to measure the effect of gas evolution were presented. The idea is based on monitoring other parameters varying due to the increase of gas release during cheese maturation. The first sensor tag configuration traces the variation of product dimension due to maturation using a resistive sensor. On the other hand, the second sensor tag configuration measures the change of pressure inside the product packaging due to gas production. The results obtained show that these two sensor RFID tag configurations can offer simple and practical solutions for controlling the cheese ripening process.

La question de la transmission intergénérationnelle du « bien manger » se trouve aujourd’hui au cœur des préoccupations des pouvoirs publics, qui souhaitent promouvoir auprès des jeunes générations une conception de la « bonne » alimentation dépassant le cadre de l’équilibre alimentaire ; or, paradoxalement, la littérature sur le rôle des acteurs familiaux dans le développement des connaissances de l’enfant liées à cette conception élargie de la « bonne » alimentation est assez succincte. A l’appui d’études documentaire, qualitative et quantitative menées auprès de parents et de grands-parents, cette recherche analyse le rôle des grands-parents, et parents, dans l’apprentissage du « bien manger » chez l’enfant. Les contributions de ce travail se situent à plusieurs niveaux (1) définition et conceptualisation du construit du « bien manger » (2) identification du rôle spécifique des grands-parents dans la transmission du « bien manger » à l’enfant (3) comparaison entre les parents et les grands-parents des représentations sociales du « repas où les enfants ont bien mangé » et des pratiques de transmission (4) analyse des variables explicatives des pratiques de transmission intergénérationnelle du « bien manger » (représentations du « bien manger », générativité, centralité du rôle parental et grand-parental, reconnexion au passé personnel). / Recently, the question of how to develop the intergenerational transmission of “eating well“ practices, conceived more broadly than “eating healthy”, has received greater attention from public policy makers. However, quite surprisingly, the academic literature in this area is rather scarce. Adopting different methodological approaches – document content analysis, qualitative analysis and quantitative analysis - , this research sheds light on how the intergenerational transmission contributes to children’s learning of “eating well” within families, and on the particular role of grandparents, in comparison with parents, in this process. Theoretical contributions are provided at different levels (1) we define and conceptualize the “eating well” construct (2) we identify the specific role of grandparents in the intergenerational transfer of “eating well” (3) we compare the structure of social representations of “a meal where children have eaten well”, as well as transmission practices, between grandparents and parents; (4) we measure the influence of relevant variables (representations, generativity, parental and grandparental role centrality and reinvolvement with personal past) on intergenerational transmission of practices related to “eating well”.

Institutions are crucial in reflecting the economic and political conditions of their time. This thesis analyses the features of establishment process of the Industrial Development Bank of Turkey (TSKB) in close relation with the World Bank and the activities of the TSKB in its first four years between 1950 and 1953. The arguments of Development economics and the dependency theory are utilized in the discussion. The main objective of the study is to bring out the link between the establishment of a privately owned development bank in Turkey, aiming to promote private industry, and the economic environment at the time, including the Cold War atmosphere affecting internal and external politics of Turkey. Moreover, it is put forward that the sectors chosen for credit allocation are compatible with the international division of labor of the time. That is, the TSKB has channeled international funds in a way aiming at Turkey&rsquo / s integration to the world markets as a supplier of agro-industrial goods.

SUMMARY Research object – consumers of ecological food products. Research subject – consumer behavior of ecological food products Research aim – to present marketing decisions stimulating purchase of ecological products. Tasks: 1.To analyze outward and inward factors impact on consumer behavior. 2.To identify marketing tools influence on consumer behavior. 3.To prepare the research methodic of ecological products consumer behavior. 4.To conduct a sample survey and to identify factors impact of purchase of ecological food products. 5.To recommend marketing decisions stimulating purchase of ecological products. Research methods - Lithuanian and foreign authors scientific literature analysis and synthesis, logical abstracts, sample survey, data grouping, method of statistical analysis, graphic presentation. Marketing decisions recommended stimulating purchase of organic products are based on Lithuanian and foreign authors theoretical principles of outward, inward and marketing factors impact on consumer behavior, sample survey, identified consumers motives of purchase and usage of ecological food products, investigated marketing tools influence to ecological food products consumer behavior.

Thesis (MSc Food Sc)--University of Stellenbosch, 2011. / ENGLISH ABSTRACT: The genus Cronobacter (Enterobacter sakazakii) contains opportunistic pathogens that can cause a severe form of neonatal meningitis, necrotising enterocolitis and septicaemia. Cronobacter infections have been reported in all age groups, however, immunocompromised infants are more susceptible to these infections. Furthermore, Cronobacter strains have been reported to show differences in sensitivity to antibiotics and virulence. These differences led to the reclassification of Cronobacter and currently the genus contains five distinct species, namely Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter turicensis, Cronobacter dublinensis and Cronobacter muytjensii. As this reclassification was only accepted recently, there are not many typing methods optimised for differentiation between the five Cronobacter species. Typing of Cronobacter strains are important as the species may be diverse regarding their virulence. Cronobacter strains have been isolated from infant formula milk (IFM), the environment of an IFM processing facility and fresh produce in South Africa. However, little is known about the phylogeny and prevalence of these strains. The aim of this study was to classify 24 South African Cronobacter strains (previously identified as E. sakazakii) and to evaluate the phylogeny of the isolates based on the 16S ribosomal RNA (rRNA) and rpoA genes. All 24 South African strains were identified as Cr. sakazakii despite a wide variety of isolation sources. Other studies have also found that irrespective of the isolation source, the majority of Cronobacter strains are identified as Cr. sakazakii. The South African strains were found to be phylogenetically closely related. However, two distinct clusters separated at a 93 % confidence level were observed in the Cr. sakazakii group based on the 16S rRNA gene analysis. Strains of Cr. sakazakii, Cr. dublinensis, Cr. turicensis and Cr. muytjensii were differentiated from each other with sequence data of the 16S rRNA and rpoA genes, but it was not possible to differentiate between Cr. sakazakii and Cr. malonaticus. The phylogram based on the rpoA gene sequences did separate Cr. malonaticus and Cr. sakazakii strains, however, the clusters were separated with a low bootstrap value of 70 %. Phylogenetic analysis based on the rpoA and 16S rRNA genes were, therefore, not sufficient to distinguish between all the Cronobacter species. The sequence data of these two genes can be used to differentiate between the Cronobacter strains when used in combination with malonate utilisation analysis. A PCR-RFLP method was subsequently developed to facilitate the simultaneous differentiation between all five Cronobacter species. The PCR-RFLP assay was based on the amplification of the rpoB gene followed by the combined digestion with restriction endonucleases Csp6I and HinP1I. Unique profiles for each of the five Cronobacter species were obtained and it was also possible to differentiate between Enterobacteriaceae and Cronobacter strains. Furthermore, two strains which were identified as Cr. sakazakii with sequencing based on the 16S rRNA and rpoA genes had PCR-RFLP profiles identical to that of Cr. malonaticus. Sequencing based on the rpoB gene and additional biochemical analysis with malonate broth confirmed the identities of these two strains as Cr. malonaticus. This PCR-RFLP assay is, therefore, an accurate typing method that ensures rapid differentiation between the five species of Cronobacter. / AFRIKAANSE OPSOMMING: Die Cronobacter genus (Enterobacter sakazakii) bevat opportunistiese patogene wat 'n ernstige vorm van neonatale meningitis, enterokolitis en septisemie kan veroorsaak. Cronobacter infeksies is al in alle ouderdomsgroepe aangemeld, maar immuungekompromitteerde babas is die meeste vatbaar vir hierdie infeksies. Verder toon Cronobacter spesies verskille in virulensie en sensitiwiteit vir antibiotika. Hierdie verskille het gelei tot die herklassifikasie van Cronobacter en tans bestaan die genus uit vyf afsonderlike spesies, naamlik Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter turicensis, Cronobacter dublinensis en Cronobacter muytjensii. Aangesien hierdie herklassifikasie slegs onlangs aanvaar is, is daar nie baie metodes wat geskik is vir onderskeiding tussen die vyf Cronobacter spesies nie. Onderskeiding tussen Cronobacter spesies is belangrik omdat die spesies verskillend kan wees met betrekking tot hulle virulensie. Cronobacter is geisoleer uit baba formule melk (BFM), die omgewing van 'n BFM fabriek en vars produkte in Suid-Afrika. Daar is egter nie baie bekend oor die filogenie en voorkoms van hierdie isolate nie. Die doel van hierdie studie was om 24 Suid-Afrikaanse Cronobacter stamme (voorheen geïdentifiseer as E. sakazakii) te klassifiseer en die filogenie van die isolate te evalueer gebaseer op die 16S ribosomale RNS (rRNS) en rpoA gene. Al 24 Suid-Afrikaanse stamme is geïdentifiseer as Cr. sakazakii ten spyte van 'n wye verskeidenheid isolasie bronne. Ander studies het ook gevind dat, ongeag die isolasie bron, die meerderheid van Cronobacter stamme as Cr. sakazakii geïdentifiseer word. In hierdie studie is gevind dat die Suid-Afrikaanse stamme filogeneties nou verwant is. Op grond van die 16S rRNA geen analise is die Cr. sakazakii stamme egter in twee afsonderlike groepe gedeel met 'n 93% vertrouens vlak. Dit was moontlik om stamme van Cr. sakazakii, Cr. dublinensis, Cr. turicensis en Cr. muytjensii van mekaar te onderskei met die DNS volgorde data van die 16S rRNA en rpoA gene, maar geen onderskeid tussen Cr. sakazakii en Cr. malonaticus stamme was moontlik nie. Die filogram gebaseer op die rpoA DNS volgorde data het aparte takke vir Cr. malonaticus en Cr. sakazakii stamme getoon, maar die twee takke is met ‘n lae vertrouens waarde van slegs 70 % geskei. Filogenetiese analise gebaseer op die rpoA en 16S rRNA gene is dus nie voldoende om te onderskei tussen al die Cronobacter spesies nie. Die DNS volgorde data van hierdie twee gene sou egter gebruik kon word om te onderskei tussen die Cronobacter spesies wanneer dit gebruik word in kombinasie met malonaatbenutting-analises. 'n Polimerase ketting reaksie (PKR) beperkings fragment lengte polimorfisme (BFLP) metode is ontwikkel om die gelyktydige onderskeiding tussen al vyf Cronobacter spesies te fasiliteer. Die PKR-BFLP metode is gebaseer op die vermeerdering van die rpoB geen gevolg deur die gesamentlike vertering met die beperkingsensieme, Csp6I en HinP1I. Unieke profiele vir elk van die vyf Cronobacter spesies is verkry en dit was ook moontlik om tussen Enterobacteriaceae en Cronobacter spesies te onderskei. Verder het twee stamme wat as Cr. sakazakii geïdentifiseer is met DNS volgordebepaling van die 16S rRNA en rpoA gene, PKR-BFLP profiele identies aan dié van Cr. malonaticus getoon. DNS volgordebepaling van die rpoB geen en ‘n addisionele biochemiese toets met malonaat sop het die identiteit van hierdie twee stamme as Cr. malonaticus bevestig. Hierdie PKR-BFLP is dus 'n akkurate metode wat vinnige onderskeid tussen die vyf spesies van Cronobacter kan verseker.