in English Germany has a long history of political fragmentation, with local and regional identities playing a crucial role in the making of Germanhood. In the last decades, historical scholarship has depicted the process of rallying local identities to the national cause. Dealing with this issue is essential because it shows the variety of the concept of Heimat [home or local homeland]1 in various territories of imperial Germany. Nevertheless, this process on the local level was not yet thoroughly examined in the case of the Giant Mountains' region before the First World War. Due to to its geographical position and the strong local cohesion shaping the homeland, this case study enables to further such historical researches, which often concentrates on the German historical regions, and not on the study of a local territory shaped by tourism. The object of the following thesis is to question the meanings assigned to the notion of Heimat in the Silesian Giant Mountains for the local activists and inhabitants, and thus, to write the history of the construction of Heimat. By using a methodology based upon different disciplines (respectively, the French "géohistoire", literary theory, sociology), the research analyses many phenomena attached to other and recent historiographic domains, such as...

Marseilleviridae é uma família de vírus gigantes cujos membros infectam amebas de vida livre. Esses vírus têm sido encontrados em amostras ambientais de água, larvas de inseto, torres de resfriamento e mais recentemente em amostras humanas. Eles possuem capsídeo icosaédrico medindo entre 190-250 nm e genoma de DNA dupla fita circular ou linear. Sua replicação ocorre no citoplasma amebiano onde observam-se fábricas virais. Este trabalho tem como objetivo investigar a presença de vírus gigantes em mexilhões-dourados (Limnoperna fortunei) que habitam o Lago Guaíba, Porto Alegre, Brasil. Quarenta indivíduos foram coletados e agrupados em pools de 5 amostras (água interna e corpo, totalizando dezesseis pools). Os pools foram homogeneizados em tampão fosfato, centrifugados e o sobrenadante filtrado em membrana de 0,45μM. Foram cultivadas amebas da espécie Acanthamoeba polyphaga em meio PYG em microplacas de 24 poços, inoculadas com os sobrenadantes, incubadas a 30ºC e examinadas diariamente em busca de efeito citopático (ECP) até 72 horas após a inoculação. Quando CPE era evidente, os sobrenadantes foram coletados e ultracentrifugados através de um gradiente de sacarose de 25%. Um dos dezesseis pools induzindo CPE claro foi submetido à extração de DNA e sequenciamento do genoma viral completo um sequenciador de nova geração (Illumina MiSeq). O genoma do vírus chamado Golden mussel marseillevirus consiste de uma única molécula de DNA de 360610 pb, com um teor de G+C de 43,1%. A análise da sequência de nucleotídeos traduzida revelou a presença de proteínas virais que apresentam homologia com proteínas de outros membros da família Marseilleviridae, como Lausanevirus e vírus Insectomime, porém grande parte do seu genoma não apresenta identidade com proteínas depositadas no banco de dados. A análise filogenética do gene D6/D11 Helicase sugere que este vírus faça parte de uma nova linhagem de marseillevirus. Este é o primeiro estudo que demonstra o isolamento de um vírus gigante a partir de tecidos de mexilhão-dourado e infere que estes vírus estão distribuídos amplamente no meio ambiente. / Marseilleviridae is a family of giant viruses whose members infect free living amoebae. These viruses have been found in environmental samples of water, insect larvae, cooling towers and, more recently, in human samples. They have icosahedral capsids measuring between 190-250 nm and their genome is double-stranded circular or linear DNA. Replication occurs in the host cell cytoplasm, inside the viral factories. This study aims to investigate the presence of giant viruses in tissues of golden mussels (Limnoperna fortunei) that inhabit the Guaiba Lake, Porto Alegre, Brazil. Forty specimens were pooled in groups of 5 specimens (internal water and body, totalizing sixteen pools). The pools were homogenized with phosphate buffer, centrifuged and the supernatant was filtered in 0,45μM. Monolayers of Acanthamoeba polyphaga were cultivated with PYG medium in 24-well microplates, inoculated with the pooled samples, incubated at 30 ºC and examined daily in search for cytopathic effect (CPE) up to 72 hours after inoculation. When CPE was evident, the supernatants were collected, clarified and ultra centrifuged through a 25% sucrose cushion. One out of the sixteen CPE positive pools was submitted to DNA extraction and complete sequencing of the viral genome in a NGS apparatus (Illumina MiSeq). The genome of the virus named Golden mussel marseillevirus consists of a single DNA molecule of 360,610 bp, with a G+C content of 43.1%. The analysis of the translated nucleotide sequence reveals the presence of proteins which are homologs to proteins predicted in other members of the family Marseilleviridae like, e.g. Lausannevirus and Isectomime virus. However, part of the viral genome has no identity with the nucleotide sequences available at the database. The phylogenetic analysis of the D6/D11 Helicase gene suggests that this virus is part of a new lineage of marseillevirus. This is the first study where the isolation of a giant virus from golden mussel tissues is achieved, suggesting that these viruses are widely distributed in the environment.

The thesis focused to population ecology and genetics of subalpine species Gentiana pannonica. Pollination ecology of species and generative reproduction in different habitat (primary vs. secondary) were investigated in the field. Genetic variation and structure of G. pannonica populations were studied in three disjunct parts of its distribution range: the Eastern Alps, the Bohemian Forest, and the Giant Mts. Present distribution of G. pannonica in the central past of the Bohemian Forest were processed.

Made available in DSpace on 2014-06-11T19:25:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-08-07Bitstream added on 2014-06-13T18:53:15Z : No. of bitstreams: 1 carli_jp_me_araca.pdf: 984712 bytes, checksum: 494c40d3d3485fa6da5828cc7b444a82 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Propósito: É propósito deste trabalho estudar a agressividade da lesão de células gigantes periférica da cavidade bucal (LCGP) através de aspectos clínicos, radiográficos, histopatológicos, histoquímicos e imunohistoquímicos, objetivando melhor conhecer o perfil clínico do paciente e, sobretudo, avaliar o comportamento biológico dessa lesão. Material e Método: Foram estudados retrospectivamente 61 casos de LCGP, procedentes da Faculdade de Odontologia de Araçatuba - UNESP e da Faculdade de Odontologia da Universidade de Passo Fundo - FO-UPF. Primeiramente, realizou-se confirmação diagnóstica histopatológica e avaliação da presença de tecido ósseo imaturo nas lesões. Após, os casos selecionados foram agrupados com base em critérios clínico-radiográficos, em difererentes graus (agressividade nula, leve, moderada, severa e extrema) e seus dados clínico-demográficos e imaginológicos foram avaliados e correlacionados. Em seguida, utilizando-se 15 casos do total estudado (05 casos com agressividade clínico-radiográfica nula, 05 com agressividade moderada e 05 com agressividade severa), investigou-se a proliferação celular das LCGPs por meio da contagem e aferição da área de AgNORs (regiões de organização nucleolar marcadas pela prata) e avaliação da expressão de PCNA (antígeno de proliferação nuclear celular), Ki-67 (Kiel-67) e p53 (proteína p53). Resultados: Dentre os resultados clínico-radiográficos obtidos destacam-se os que dizem respeito à idade dos pacientes (prevalência entre 31 e 40 anos), sexo (prevalência em mulheres), raça (prevalência em brancos) e localização anatômica (prevalência na região mandibular anterior). A maioria das lesões se mostrou como nódulo indolor, de superfície lisa, dois meses de evolução, consistência fibrosa, formato arredondado ou ovóide, coloração púrpura e base séssil... / Purpose: To study the aggressiveness of peripheral giant cell lesion of buccal cavity (PGCL) by means of clinical, radiographic, histopathological, histochemical and immunohistochemical aspects in order to determine the patient's profile and to evaluate the biologic behavior of this lesion. Material and Method: 61 cases of PGCL of patients from Faculdade de Odontologia de Araçatuba - UNESP and Faculdade de Odontologia da Universidade de Passo Fundo - FOUPF were studied retrospectively. A corroborative diagnostic and the evaluation of immature bony tissue in the lesions were accomplished. These cases were divided into different groups according a classification based on grades of clinical-radiographic aggressiveness. The data of each group were evaluated and correlated and 15 cases were selected (5 cases with null clinical-radiographic aggressiveness, 5 with moderate aggressiveness and 5 with severe aggressiveness). An investigation of the cellular proliferation of PGCL was accomplished by count and measurement of the area of AgNORs (nucleolar organization regions marked by silver) and evaluation of PCNA (proliferating cell nuclear antigen), Ki-67 (Kiel-67) and p53 (protein p53). Results: The following conclusions were drawn by the clinical-radiographic results: in respect to the patient's age (prevalence between 31 and 40 years old), sex (prevalence in women), race (prevalence in white people), anatomical location (prevalence in the mandibular anterior region). Clinically the lesions were predominantly observed as painless tissue growths of smooth surface, two months of evolution, fibrous consistence, nodular form, purple coloration and sessile basis of implantation. In the greater number of cases the radiographic image of the subjacent bony tissue to the PGCL... (Complete abstract, click electronic address below)

Made available in DSpace on 2014-06-11T19:32:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-28Bitstream added on 2014-06-13T20:03:28Z : No. of bitstreams: 1 babeto_e_dr_sjrp.pdf: 649918 bytes, checksum: 812ddac8aa99c1d4ba6dfcdc980015a8 (MD5) / O tumor ósseo de células gigantes (TCG) é um tumor benigno, que causa destruição osteolítica, com comportamento biológico incerto e com características particulares como um elevado número de células gigantes multinucleadas e comportamento agressivo. A recorrência local do TCG é freqüentemente observada em 20 a 50% dos casos. Mais agravante que a recorrência, é o fato de que após a recidiva, o paciente muitas vezes também apresenta metástases em outros órgãos, principalmente no pulmão. Dessa forma, o objetivo do trabalho foi á investigação da expressão gênica para identificar genes diferencialmente expressos no TCG, que podem estar envolvidos na biologia molecular e desenvolvimento da doença. A hibridização subtrativa rápida (RaSH) foi utilizada para identificar novos genes diferencialmente expressos, como KTN1, NEB, ROCK1 e ZAK, que foram validados por PCR quantitativo em tempo real (qPCR) e a imuno-histoquímica em amostras de TCG comparadas ao tecido ósseo normal. A anotação funcional indica que estes genes estão envolvidos em processos celulares relacionadas ao fenótipo tumoral. A expressão dos genes KTN1 e ROCK1 encontra-se aumentada e o gene ZAK tive sua expressão reduzida nas amostras de TCG analisadas. Pela presença de ilhas CpG na região promotora e baixa expressão no tecido tumoral, o padrão de metilação do gene ZAK foi analisado por MSP-PCR. Os genes identificados KTN1, ROCK1 e ZAK pode ser responsável pelas perda de homeostase celular no TCG uma vez que são responsáveis pela várias funções relacionadas com a tumorigênese, como a migração celular, organização do citoesqueleto, apoptose, controle do ciclo celular e, portanto esses resultados poderão contribuir para a compreensão das bases moleculares do TCG, assim ajudando a melhorar o diagnóstico, prognóstico e tratamento dos pacientes / Giant cells tumors of bone (GCTB) are benign in nature but cause osteolytic destruction, with a number of particular characteristics. These tumors can have uncertain biological behavior, often contain a significant proportion of highly multinucleated cells, and may show aggressive behavior. We have studied differential gene expression in GCTB that may give a better understanding of their physiopathology, and might be helpful in prognosis and treatment. Subtractive hybridization (RaSH) was used to identify and measure novel genes that appear to be differentially expressed, including KTN1, NEB, ROCK1 and ZAK, using qRT-PCR and immunohistochemical in the samples of GCTBs compared to normal bone tissue. Normal bone was used in the methodology RaSH for comparison with the GCTB in identification of differentially expressed genes. Functional annotation indicated that these genes are involved in cellular processes related to their tumor phenotype. The differential expression of KTN1, ROCK1 and ZAK was independently confirmed by qRT-PCR and immunohistochemical. The expression of the KTN1 and ROCK1 genes were increased in samples by qRT-PCR and immunohistochemical and ZAK had reduced expression. Since ZAK have CpG islands in their promoter region and low expression in tumor tissue, their methylation pattern was analyzed by MSP-PCR. The genes identified, KTN1, ROCK1 and ZAK may be responsible for loss of cellular homeostasis in GCTB since they are responsible for various functions related to tumorigenesis such as cell migration, cytoskeletal organization, apoptosis, cell cycle control and thus may contribute at some stage in the process of formation and development of GCTB

abstract: A theoretical study of a three-dimensional (3D) N/S interface with arbitrary spin polarization and interface geometry is presented. The 3D model gives the same intrinsic spin polarization and superconducting gap dependence as the 1D model. This demonstrates that the 1D model can be use to t 3D data. Using this model, a Heusler alloy is investigated. Andreev reflection measurements show that the spin polarization is 80% in samples sputtered on unheated MgO(100) substrates and annealed at high temperatures. However, the spin polarization is considerably smaller in samples deposited on heated substrates. Ferromagnetic FexSi􀀀x alloys have been proposed as potential spin injectors into silicon with a substantial spin polarization. Andreev Reflection Spectroscopy (ARS) is utilized to determine the spin polarization of both amorphous and crystalline Fe65Si35 alloys. The amorphous phase has a significantly higher spin polarization than that of the crystalline phase. In this thesis, (1111) Fe SmO0:82F0:18FeAs and Pb superconductors are used to measure the spin polarization of a highly spin-polarized material, La0:67Sr0:33MnO3. Both materials yield the same intrinsic spin polarization, therefore, Fe-superconductors can be used in ARS. Based on the behavior of the differential conductance for highly spin polarized LSMO and small polarization of Au, it can be concluded that the Fe-Sc is not a triplet superconductor. Zero bias anomaly (ZBA), in point contact Andreev reflection (PCAR), has been utilized as a characteristic feature to reveal many novel physics. Complexities at a normal metal/superconducting interface often cause nonessential ZBA-like features, which may be mistaken as ZBA. In this work, it is shown that an extrinsic ZBA, which is due to the contact resistance, cannot be suppressed by a highly spin-polarized current while a nonessential ZBA cannot be affected the contact resistance. Finally, Cu/Cu multilayer GMR structures were fabricated and the GMR% measured at 300 K and 4.5 K gave responses of 63% and 115% respectively. Not only do the GMR structures have a large enhancement of resistance, but by applying an external magnetic eld it is shown that, unlike most materials, the spin polarization can be tuned to values of 0.386 to 0.415 from H = 0 kOe to H = 15 kOe. / Dissertation/Thesis / Doctoral Dissertation Physics 2015

Les virus sont ubiquitaires et abondants dans l’environnement. Ils influent fondamentalement sur l’écologie de l’ensemble des écosystèmes et du microbiote humain. Dès 2002, avec la découverte de virus géants d’amibes, la virologie s’est complexifiée. Les Megavirales (nouvel ordre au sein des grands virus nucléocytoplasmiques) ont 10% de gènes homologues aux cellules eucaryotes, et ont la caractéristique singulière d’être infectés par des virophages.Avec l’avènement de la métagénomique, le nombre de métagénomes produits ne cesse de croître de manière exponentielle. C’est ainsi que la virologie a connu un nouvel essor et a pu mieux être étudiée en s’affranchissant des difficultés de culture et d’isolement des virus dans les conditions artificielles de laboratoire. La métagénomique permet d’étudier les communautés microbiennes mais également de découvrir de nouveaux microbes. La bioinformatique est devenue incontournable dans le domaine de la biologie et essentielle pour les biologistes afin de traiter les masses de données et en extraire toute la richesse de l’information biologique nécessaire. La première partie de cette thèse consiste en une revue de la littérature décrivant la bioinformatique au service de la métagénomique virale. La deuxième partie présente la création d’un nouvel outil « MG-Digger » dédié à l’analyse rapide et automatisée de séquences d’intérêts spécifiques dans les métagénomes. La dernière partie se concentre sur l’utilisation de cet outil sur des données issues de projets métagénomiques afin de répondre à des questions biologiques précises, notamment sur les données de l’expédition scientifique TARA à travers les océans. / Viruses are ubiquitous and abundant in the environment and can influence all ecosystems ecology and the human microbiota.Since 2002, with the discovery of giant viruses of amoeba, virology has become more complex and the definition of virus has been called into question, not only because of their phenotypic sizes similar to those of bacteria but also their genomic content exceeding thousand genes. Megavirales, also known as nucleocytoplasmic large DNA viruses, have 10% homologous genes to eukaryotic cells and interestingly can be infected by virophages. With the advent of metagenomic, the number of metagenomes produced is exponentially increasing as well as our understanding of virology which has been studied. Metagenomics studies showed an efficient way to study microbial communities and identify novel viruses without the difficulties of culture and isolation of viruses in artificial laboratory conditions.Metagenomic requires considerable computational and storage resources (Big data processing). Therefore, bioinformatics has become an integral part of research and development in the biomedical sciences by providing tools that handle complex datasets and finally giving the necessary biological information. The first part of this thesis consists of an exhaustive review of the literature describing bioinformatics and viral metagenomics. The second part presents a new "MG-Digger" tool dedicated to the rapid and automated analysis of specific interest sequences in metagenomes. The third part focuses on the use of this tool on metagenomic data to answer to specific biological questions, including data from the TARA scientific expedition across the oceans.

Este trabalho teve como finalidade determinar e otimizar as condições para a preparação e a caracterização de monocristais de alguns óxidos que apresentam considerável dependência de suas propriedades físico-químicas finais e magnitude da pressão da atmosfera de crescimento. Experimentos que envolvem diversas adaptações de um sistema de fusão a laser de pedestais foram projetados para investigar o melhor crescimento de monocristais de molibdatos alcalinos isolados e mistos (CaMoO4, cristal gradiente de CaMoO4-SrMoO4, Ca2</subFeMo06) e de um óxido titanato alcalino (Ba0,77Ca0,23TiO3). Também, os aspectos teóricos das condições experimentais foram estudados nos termos de um modelo termodinâmico existente. Adicionalmente, é mostrado que técnicas de caracterização estruturais (curvas de rocking com raios-X, topografia de raios X, método de Rietveld), térmicas (análise térmica diferencial) e ópticas (absorção ótica) foram aplicadas com sucesso além de outros métodos de caracterização bem estabelecidos (fotografia de retro-reflexão de raios-X, análise por raios-X de elétrons retro-espalhados, microscopia eletrônica de varredura). Os experimentos e os dados das caracterizações revelam que CaMoO4, SrMoO4 e Ba0,77Ca0,23 podem ser crescidos ao ar sem alterações de suas propriedades quando tratamento térmico cuidadoso é realizado nos reagentes de partida e aos produtos, enquanto que o Ca2FeMoO6 somente pode ser crescido em atmosfera isostática de N2 e em uma faixa pequena de pressões (0,25-0,75 bar). Em qualquer caso, a estabilidade do processo de crescimento é alcançada quando gradientes térmicos específicos e relações geométricas na zona fundida são estabelecidos após a imposição de uma atmosfera adequada de crescimento. As medidas estruturais, térmicas, ópticas e de composição convencionais confirmam a formação de fases e estequiometrias desejadas. Os dados estruturais refinados e de alta resolução demonstram o controle da composição e conseqüentemente do espaçamento interplanar de rede no cristal gradiente CaMoO4-SrMoO4, um primeiro exemplo de cristal gradiente de um óxido crescido por uma técnica de zona flutuante, e abrindo um panorama largo de possibilidades para a preparação de outros cristais gradientes de óxidos. / This thesis examines the conditions for the optimized single crystal preparation and characterization of some oxides compounds that present high dependency of the nature and pressure magnitude of the growth atmosphere on their final physico-chemical properties. Experiments involving several adaptations to a laser-heated pedestal growth system were designed to investigate the best crystal growth of a mixed alkaline titanate oxide (Ba0,77Ca0,23TiO3) and single and mixed alkaline molybdates oxides (CaMoO4, cristal gradiente de CaMoO4-SrMoO4, Ca2</subFeMo06). AIso, theoretical aspects of the experimental conditions were studied in terms of an existent thermodynamical model. In addition, it is shown that non-standard to techniques to characterize crystal\' s structural features (X-ray rocking, X-ray topography, Rietveld\'s method), thermo-physical (Differential Thermal Analysis) and optical properties (Optical Absorption) were succesfully applied besides others well established characterization methods. (X-ray Laue backreflection photography, Electron Dispersive X-Ray Analysis, Scanning Electron Microscopy). Experiments and characterizations data reveal that CaMoO4, SrMoO4 e Ba0,77Ca0,23succesfully grow in air at room conditions when careful thermal treatment of the starting materiaIs and products, while Ca2FeMo06 only can be grown in isostatic atmosphere of N2/H2 (95&#8240 vol./5&#8240 voI.) and at a short pressure range (0.25-0.75 bar). In any case, the stability of the growth process is reached when specific thermal gradients and geometric relations in the meU are established after the imposition of an adequate growth atmosphere. Conventional structure, thermal, optical and composition measurements confirm the formation of desired phases and stoichiometries with excellent results for both single and mixed compounds. Refined and high resolution structural data demonstrate large control of composition and therefore interplanar lattice spacing in the gradient crystal CaMoO4-SrMoO4, a first example of an oxide gradient crystal grown by a floating zone technique, and open a wide panorama of possibilities for the preparation of novel gradient oxides crystals.

A expressão da conexina 32 e 43 nas células gigantes trofoblásticas foi analisada em condições de cultura celular com e sem influência de hormônios sexuais. Placentônios bovinos foram coletados de vacas prenhes em abatedouro nos diferentes períodos gestacionais e divididos em três grupos, primeiro terço (I), segundo terço (II), terceiro terço (III) e transportados ao laboratório em condições assépticas à temperatura de 4ºC em solução de PBS com antibiótico. No laboratório as células foram isoladas e cultivadas em meio D-MEM com 10% SFB por cinco dias. As detecções das conexinas 32 e 43 foram realizadas através de munofluorescencia, pelo método de amplificação da tiramida-fluoresceina, utilizando anticorpo primário policlonal a imunoglobulina de coelho, anti-conexina 32 e 43 de camundongo. Os resultados mostraram que as células gigantes trofoblásticas expressam conexinas 32 e 43 nos três períodos gestacionais com exceção da Cx32 no primeiro terço gestacional sem adição de hormônios, a qual passou a expressar fluorescência após a adição de hormônios. A distribuição da Cx43 evoluiu com a progressão da gestação, permanecendo limitada ao interior das células gigantes trofoblásticas, sem formar junções comunicantes. O terceiro terço gestacional mostra a Cx43 na CGTT localizada no interior do núcleo. A adição de hormônios ao meio de cultura vem confirmar que a progesterona e o estrógeno podem ter um papel no controle da Cx32. Para Cx43 onde se adicionou progesterona os níveis de expressão foram baixos no início aumentando no decorrer da gestação, o mesmo foi encontrado para o conjugado de progesterona/ estrógeno. Para o estrógeno no grupo I os níveis de expressão foram menores se comparados aos três grupos diminuindo no grupo II e voltando a aumentar no grupo III. / The trophoblast cells expression of connexins 32 and 43 was studied in cell culture conditions with or without influence of sexual hormones. Bovine placentomes were collected from pregnant cows in slaughterhouses in different gestational periods and so divided into three groups, first term (I), second term (II), and third term (III), and transported to the laboratory in aseptic conditions at a temperature of 40C in PBS solution with antibiotics. At the laboratory the cells were isolated and cultivated in D-MEM environment with 10% SFB for five days. The detections of connexins 32 and 43 were achieved through immunofluorescence, by the tyramide-fluorescein amplification method, using a rabbit polyclonal primary antibody anti-connexin 32 and 43 of mice. The results showed that the trophoblast cells expressed connexins 32 and 43 in the three gestational periods with an exception of the Cx32 at the first gestational period. However, after the addition of sexual hormones, they began to express the connexins in the cytoplasm in the first stage. The distribution of the Cx43 evolved with the progression of the gestation, remaining limited to the trophoblast`s cells interior, without formation of gap junctions. The third gestational term shows the Cx43 located inside the nuclei of the CGTT. Addition of hormones in the culture environment confirmed that estrogen and progesterone may have an important action controlling Cx32. For Cx43, the expression was low after prosterone was added to the culture medium, but increased as the gestation evolved, the same was found for a combined compost of estrogen\\ progesterone. For estrogen, in group I the expression levels were inferior when compared to the three groups decreasing in group II and increasing again in group III. We may conclude that the sexual hormes, specially estrogen, affect the expression of connexins in trophoblastic cells.

A lesão central de células gigantes (LCCG) é uma afecção benigna dos maxilares, de comportamento biológico incerto, variando de discreta tumefação assintomática e de crescimento lento à uma forma agressiva, associada a dor, reabsorção radicular e óssea, com destruição cortical. Sua etiologia permanece desconhecida, havendo controvérsias entre processo reacional, neoplásico ou genético. Mutações no gene SH3BP2 foram identificadas em pacientes com querubismo, condição que compartilha várias características clínicas, radiográficas e histopatológicas com a LCCG. Para testar a hipótese de que tais mutações seriam responsáveis por, ou estariam associadas a LCCG e na tentativa de melhor entender a diferenciação microscópica/morfométrica das lesões agressivas e não agressivas, vinte e cinco pacientes portadores de LCCG foram selecionados para o estudo. O DNA foi obtido através do sangue e de espécimes em blocos de parafina, oriundos de biópsias e tratamento cirúrgico. Um estudo microscópico morfométrico foi paralelamente realizado, para avaliar o número de células gigantes e densidade de volume das mesmas nas lesões agressivas e não agressivas. O sequenciamento genético dos treze exons do gene SH3BP2 nos vinte e cinco pacientes estudados evidenciou uma alteração no códon do exon 4 em 10 pacientes. A densidade de volume de células gigantes foi maior nas lesões agressivas quando comparadas às não agressivas (p=0,013). Não houve diferença significante quanto ao número de células gigantes/mm2 em lesões agressivas e não agressivas (p =0,245). / Central giant cell lesion (CGCL) is a benign disease of the jaws, with uncertain behavior, ranging from mild asymptomatic slow-growing swelling to an aggressive form, with pain, radicular and bone resorption and cortical destruction. Its aetiology is still unknown and there is discussion whether it is a reactive, neoplastic or genetic disease. Mutations on gene SH3BP3 were identified in patients with cherubism, which shares several clinical, radiographic and histopathological features with CGCL. In order to test the hypothesis that such mutations would be responsible for or would be related to CGCL and also in order to better understand microscopic morphometric differentiation of the aggressive and non-aggressive lesions, 25 patients with CGCL were selected to this study. DNA was extracted from blood samples and from tissue samples, obtained by biopsy or surgical treatment. Microscopic morphometric assessment was also performed, in order to evaluate the number and the volume density of the giant cells in aggressive and in non-aggressive lesions. Gene sequencing of all 13 exons in gene SH3BP3, performed on each of the 25 patients, showed an alteration in one codon from exon 4, in ten patients. Volume density of giant cells was greater in aggressive lesions than in non-aggressive ones (p=0,013). There was no significant difference on the number of giant cells per mm2 when comparing aggressive and non-aggressive lesions.