This Master's thesis deals with an analysis and subsequent comparison of outplacement tools used in Czech company with thouse which are applied in international organization. Analysis has been conducted in a Czech company První novinová společnost a.s. and in an international company Unilever. Apart from analysing outplacement tools the thesis also looks at the approach of both organizations toward outplacement.

Na avicultura de postura, a associação dos aminoácidos e minerais quelatados pode afetar positivamente a composição química dos ovos e o colesterol da gema. Desta forma, em dois ensaios experimentais distintos, objetivou-se com avaliar a interação do nível dietético de metionina e cistina com o cobre quelatado e treonina e zinco quelatado sobre a composição química do ovo. Para tanto, foram utilizados em cada ensaio 320 poedeiras comerciais, distribuídas sob o delineamento inteiramente casualizado. Os tratamentos, distribuídos aleatoriamente em quatro repetições, foram organizados em arranjo fatorial 5 x 4: sendo o primeiro fator teor de aminoácido e o segundo nível do mineral quelatado, constituindo 20 tratamentos, com quatro repetições e quatro aves por unidade experimentais. A parcela experimental era constituída de quatro aves com 48 semanas de idade. No primeiro ensaio os níveis totais de metionina+ cistina (met+cys) eram: 0,613, 0,631, 0,816, 0,918 e 0,955 % e os de cobre (Cu): 18, 44, 71 e 99 mg / kg. No segundo, eram: níveis totais de treonina (Thr) 0,614, 0,698, 0,875, 1,006, 1,055%; e. zinco (Zn): 31, 73, 104 e 121 mg / kg. Os níveis avaliados foram estabelecidos após análises laboratoriais. Em ambos os experimentos, foram avaliados: ovos e conteúdos, taxas diárias de deposição, composição química dos conteúdos na Matéria Natural (MN) e Matéria Seca (MS). Para as determinações da composição química PB%, EE%, MM%, H2O% e colesterol dos ovos, foram colhidos dois ovos por parcela. Os dados foram analisados utilizando-se o procedimento SISVAR 5.6 e o nível de significância considerado foi de 5%. No ensaio 1, o efeito de interação (P < 0,05) entre Met + Cys*Cu, foi observado para maioria das variáveis avaliadas. Entretanto, foram observados efeito principal (P < 0,05) de Met+ Cys para as seguintes variáveis: peso de casca e gema; deposição de Nitrogênio (N), Extrato Etéreo no Albúmen (EE no Alb) e Cinzas, composição química do ovo nas Matérias Natural e Seca; Proteína Bruta (PB), Extrato Etéreo (EE) e Cinzas no Albúmen, ovo reconstituído e MS no Alb, e para colesterol total. O efeito do aumento nos níveis de Met + Cys resultou em acréscimo de 18,17 % no teor de colesterol. Ensaio 2: Foi observado efeito de interação (P <0,05) entre Thr* Zn, para maioria das variáveis analisadas. Todavia, foi observado efeito principal (P< 0,05) do aminoácido para as seguintes variáveis: pesos de casca e gema, na composição química do ovo na MN (EE na gema), onde o aumento nos níveis de Thr resultaram no aumento do EE na gema, para o colesterol total. Conclui-se que em ambos os experimentos a utilização mineral quelatado em dietas para poedeiras pode trazer benefícios para qualidade do ovo, melhorando alguns parâmetros da composição química do ovo. / In laying hens production, an association of amino acids and chelated minerals can positively affect a chemical composition and yolk cholesterol.of the eggs. Thus, the objective was to evaluate the interaction of the dietary level of methionine and cystine with the chelated copper and chelated threonine and zinc on the chemical composition of the egg, in two different experimental trials. For this, 320 commercial layers were used in each experiment, distributed under a completely randomized design. The treatments, were randomly distributed in four replications, in a 5 x 4 factorial arrangement of treatments, with five levels of the factor amino acid content and four levels of the factor chelated mineral concentration, constituting 20 treatments, with 4 replicates and 4 layers per experimental unit. The experimental plot consisted of four birds with 48 weeks of age. In the first trial, the total methionine + cystine (Met + Cys) levels were: 0.613, 0.631, 0.816, 0.918 e 0.955% and cooper (Cu): 18, 44, 71 e 99 mg / kg. In the second trial, the total threonine (Thr) levels were: 0.614, 0.698, 0.875, 1.006, 1.055%, and zinc (Zn): 31, 73, 104 e 121 mg / kg. The levels assessed were established after laboratory analysis. In both experiments, were evaluated: eggs and contents, daily rates of deposition, chemical composition of contents in natural matter (NM) and dry matter (DM). For the determinations of the chemical composition (PB%, EE%, MM%, H2O% and cholesterol of the eggs), two eggs were collected per plot. Data were analyzed using the SISVAR 5.6 procedure and the level of significance considered was 0.05. Experiment 1: the interaction effect (P < 0.05) between Met + Cys*Cu was observed for most of the evaluated variables. However, the main effect of Met + Cys was observed (P < 0.05) for the following variables: egg shell and yolk weight; deposition of nitrogen (N), ether extract (EE) in albumen and ash; chemical composition of the egg in natural and dry matter (CP, EE and Ash in the albumen, reconstituted egg and DM in albumen) for the total cholesterol. The effect of the increase in a Met + Cys levels resulted in an increase of 18.17% in the cholesterol content. Experiment 2: the interaction effect (P < 0.05) between Thr* Zn was observed for most of the observed variables. However, the main effect (P < 0.05) of the amino acid was observed for the following variables: egg shell and yolk weights, in the chemical composition of the egg in the NM (EE yolk), where the increase in Thr levels resulted in increased of yolk EE, Therefore, it is concluded that in both experiments the chelated mineral utilization in laying diets can bring benefits to egg quality, improving some parameters of the egg chemical composition.

Ziel dieser Arbeit war eine Untersuchung zur Häufigkeit des Wechsels von Legehennen zwischen Innen- und Außenbereichen, einschließlich der Aufenthaltsdauer außerhalb des Stalles, in Relation zum Federpicken und der Beschaffenheit des Integumentes. Der Einfluss der Haltungsbedingungen und der Einfluss des Genotyps wurden untersucht. Die Gruppe, die ohne Grünauslauf war, bewegte sich signifikant häufiger zwischen dem Stall und dem Wintergarten als andere Gruppen mit Grünauslauf. Die Aufenthaltsdauer im Stall und Wintergarten war in der Gruppe ohne Grünauslauf signifikant höher als bei anderen mit Grünauslauf (GA). Hennen, denen eine große Auslauffläche angeboten wurde (10m²/T) bewegten sich signifikant häufiger zu den Außenbereichen als andere mit einem Angebot von 2,5 m²/Tier. Hennen mit einem Angebot von 2,5 m²/Tier hielten sich länger auf dem Grünland auf als Hennen mit einer Besatzdichte von 10m²/T. Die höchsten Federpickaktivitäten waren in der Gruppe ohne GA im Vergleich zu den Gruppen mit GA. Im Vergleich zu anderen Gruppen mit GA, hatte die Gruppe ohne GA einen schlechteren Gefiederzustand. LSL Hennen bewegten sich im Vergleich zu den LT Hennen häufiger zwischen Innen- und Außenbereichen. LSL Hennen hielten sich im Vergleich zu den LT Hennen länger im Wintergarten auf. Dennoch hielten sich die LT Hennen im Vergleich länger auf dem Grünland auf. Die schlechteste Gefiederbeschaffenheit wiesen LSL Hennen im Vergleich zu LT Hennen auf. Die Resultate der vorliegenden Untersuchungen weisen darauf hin, dass die Auslaufnutzung einen positiven Einfluss auf den Gefieder- und Hautzustand hat. Die untersuchten Genotypen unterschieden sich in der Häufigkeit des Ortswechsels und der Aufenthaltsdauer im Grünauslauf. / The aim of this work was to investigate the frequency of movement of laying hens between indoor and outdoor enclosures and the time hens spent outside the hen house in relation to feather pecking and condition of the integument. The effect of housing condition and the genotype were studied. Hens that were offered a large space to outside open area (10m²/bird) moved significantly more frequently to the outdoor areas than others (2.5m²/bird). Frequency of movement between poultry house and winter garden was higher in the group without grassland. Hens kept at a higher density outdoors (2.5m²/bird) spent significantly more time in this area than hens were kept at a lower density (10m²/bird) on grassland. The high rates of feather pecking are observed in the group without grassland compared to groups stocked at 2.5m² or 10m² per bird on grassland The group without grassland had poorer feather condition compared to other groups that had 2.5m²/bird and 10m²/bird on grassland Lohmann Selected Leghorn (LSL) hens moved more frequently to the outdoor enclosures compared to Lohmann Tradition (LT). But LT hens spent significantly more time on grassland than LSL.The plumage condition was worse in LSL than LT hens. It is concluded that housing conditions might have a stronger influence on the development of feather pecking than the genetic aspect. Large differences between the genotypes examined were found in respect to the frequency of movement to the outdoor areas, the time spent outside and plumage and skin damage.

FAD * for Stadsgårdskajen Re-introduction of some of the knowledge from the master builder to the contemporary architect. This project investigates the interaction between designing architecture and building architecture in a computer controlled context. Algorithms have been developed to simulate and control an automated in situ brick stacking process. Brick laying is a well researched topic for robotic processes. It is an ideal material for building with robots (in Stockholm) since it is a generic building block that is cheap and easy to produce locally. Corbeled vaults, instead of keystone vaults, can be built without support and with all identical elements and are therefore also suitable for robotic fabrication.  The algorithms have been incorporated in a parametric model that have been used to develop public building at Stadsgårdskajen, Stockholm. FAD is about creating and explore a different architectural expression as well as reintroducing the brick as a relevant construction material for Stockholm. / FAD* för Stadsgårdskajen Återinförande av några av byggmästarens förmågor till den samtida arkitekten I detta examensarbete undersöks förhållandet mellan att designa arkitektur och att bygga arkitektur i en datorkontrollerad miljö. Algoritmer har utarbetats för att simulera och styra en automatiserad in situ murningsprocess. Inom fabrikation och automatisering med industrirobotar är tegelband ett välundersökt ämne. Tegel är ett idealiskt material vid byggande med robotar. Det är enkelt och billigt att framställa och tegel som byggkloss är en lämplig typologi för addativ fabrikation med robotar. Valv byggda genom utkragning istället för traditionella valvbågar kan byggas utan stöd och med identiska element. Därför är denna byggnadsteknik även lämplig för additiv fabrikation med robotoar. Algoritmerna har intergrerats i en parametrisk modell som har använts för att formge en offentlig byggnad på Stadsgårdskajen i Stockholm. FAD handlar såväl om skapande och utforskande av ett nytt arkitekturspråk som av återinförande av tegel som ett betydelsefullt konstruktionsmaterial i Stockholm.

In Deutschland werden jährlich über 40 Millionen männliche Eintagsküken aus Legelinien aufgrund vorrangig wirtschaftlicher Interessen getötet. Dies stellt sowohl ein ethisches als auch ein tierschutzrechtliches Problem dar (ANON. 2006, IDEL 2007). Gerade vor dem Hintergrund aktueller politischer Entscheidungen (MUNLV NRW 2013, NI MELV 2014) besteht ein Bedarf an Alternativen zur Tötung männlicher Eintagsküken. Verschiedene Lösungsansätze wie z. B. das Zweinutzungshuhn (ICKEN et al. 2013) oder aber die Mast männlicher Geschwisterhühner (KAUFMANN und ANDERSSON 2013) sind derzeit aus ökonomischen und ökologischen Gründen nicht flächendeckend realisierbar. Eine weitere Möglichkeit bietet die In-ovo-Geschlechtsbestimmung. Hierbei wird das embryonale Geschlecht bereits vor dem Schlupf identifiziert; nachfolgend können die Eier mit männlichen Embryonen aussortiert werden. Um sowohl ethischen als auch tierschutzrechtlichen Aspekten Genüge zu tun, sollte die Geschlechtsidentifikation dabei vor Einsetzen des embryonalen Schmerzempfindens stattfinden (Tag 10 + 12 h der Bebrütung; CLOSE et al. 1997). Ziel der vorliegenden Arbeit war die Entwicklung einer verlässlichen Methode zur In-ovo-Geschlechtsbestimmung anhand geschlechtsspezifischer Differenzen im Hormongehalt der Allantoisflüssigkeit sieben bis zehn Tage alter Hühnerembryonen. Nachfolgend wurde der Einfluss der Geschlechtsbestimmung auf die embryonale Entwicklung, Schlupferfolg, Aufzucht sowie die Leistungsparameter der adulten Tiere analysiert. Im Rahmen der ersten Teilstudie erfolgte die Beprobung von n = 750 Eiern des Braunlegehybrids Lohmann Brown (LB, Lohmann Tierzucht GmbH, Deutschland). Der minimalinvasiven Entnahme von Allantoisflüssigkeit folgte die Untersuchung auf 17β-Östradiol (E2), Östronsulfat (E1S) und Testosteron mittels an das Haushuhn angepassten Enzymimmunoassays (ELISA). Es konnten sowohl für E2 als auch für E1S signifikante (p < 0,01) geschlechtsspezifische Differenzen in der Allantoisflüssigeit von neun und zehn Tage alten Embryonen nachgewiesen werden. Die Testosteronkonzentration hingegen zeigte an keinem der untersuchten Tage geschlechtsabhängige Unterschiede und erwies sich somit für die In-ovo-Geschlechtsbestimmung als ungeeignet. Die statistische Auswertung ergab, dass die Bestimmung von E1S eine frühere und genauere Geschlechtsidentifikation ermöglicht als die von E2. Der für E1S festgelegte Grenzwert erreicht bei neun Tage alten Embryonen eine 86%ige Sensitivität und 83%ige Spezifität. In der zweiten Teilstudie wurde die zuvor etablierte Technik der Geschlechtsbestimmung mittels E1S an 8 + 4 h (n = 2420) und 9 + 4 h (n = 2850) Tage alten Embryonen der Herkunft LB sowie an n = 150 9 + 4 h alten Embryonen des Weißlegehybrids Lohmann Selected Leghorn (LSL, Lohmann Tierzucht GmbH, Deutschland) überprüft. Das Geschlecht der 8 + 4 h Tage alten Embryonen konnte zu 84 % korrekt identifiziert werden. Dieser Wert stieg bei 9 + 4 h Tage alten Embryonen auf 98 % (LB) bzw. 100 % (LSL) an. Im Vergleich zu einer unbehandelten Kontrollgruppe (n = 5258) wurde die Schlupfrate durch die Entnahme von Allantoisflüssigkeit um 1,4 - 3,5 (LB) bzw. 12,7 Prozentpunkte (LSL) reduziert. Nachfolgend wurden 150 Tiere der Versuchsgruppe und 80 Tiere der Kontrollgruppe für eine Aufzuchtperiode von 17 Wochen eingestallt. Hierbei zeigten sich hinsichtlich des Körpergewichtes signifikante (p < 0,05) Unterschiede zwischen Versuchs- und Kontrollgruppe in Woche 4 und 6, wobei die Zunahmen in der Versuchsgruppe geringer waren. Anschließend wurde die Leistung von 120 Tieren der Versuchsgruppe und 60 Tieren der Kontrollgruppe bis Lebenswoche 33 bezüglich Legeleistung, Eigewicht, Körpergewicht sowie Futterverbrauch analysiert. Bei keinem der untersuchten Parameter konnten signifikante Unterschiede zwischen den Gruppen festgestellt werden (p > 0,05). Die Resultate der vorliegenden Arbeit zeigen, dass eine verlässliche Geschlechtsbestimmung in ovo bei 9 + 4 h Tage alten Hühnerembryonen mithilfe einer Bestimmung der E1S-Konzentration in der Allantoisflüssigkeit möglich ist; zudem ist die beschriebene Methode bei verschiedenen Legelinien anwendbar. Die Entnahme von Allantoisflüssigkeit führt zwar zu einer minimalen Reduktion der Schlupfrate, bei adulten Legehennen kommt es jedoch zu keiner Beeinträchtigung der Produktionsleistung. Demnach erfüllt das etablierte Verfahren alle Grundvoraussetzungen für eine Anwendung in kommerziellen Brütereien. Da die Geschlechtsbestimmung vor Einsetzen des embryonalen Schmerzempfindens erfolgt, kann sie somit als Grundlage für eine ethisch vertretbare Alternative zum Töten männlicher Eintagsküken angesehen werden. / In Germany about 40 million day-old male chicks are culled each year predominantly because of economic reasons. From the animal welfare as well as the ethical point of view this is a problematic situation (ANON. 2006, IDEL 2007). Particularly with regard to current political decisions (MUNVL NRW, NI MELV 2014) alternatives to the culling of male day-old chicks are required. Different approaches such as a dual-purpose breed (ICKEN et al. 2013) or the fattening of male layer-hybrids (KAUFMANN and ANDERSSON 2013) are not ubiquitous marketable at present due to economic and ecological reasons. In ovo sexing represents another option; the embryonic gender is determined before hatch and the eggs containing male embryos can be eliminated subsequently. To comply with ethical and animal welfare aspects, the sexing should take place before the onset of embryonic pain perception (embryonic day 10 + 12 h; CLOSE et al. 1997). Aim of this thesis was the development of a reliable method for in ovo gender identification with the help of sex-specific differences in the hormone concentration of the allantoic fluid of seven to ten day old chick embryos. Subsequently, the influence of gender identification on embryonic development, hatching rate, rearing as well as production performance of the adult hens was analysed. Within the first study n = 750 eggs of the brown layer-hybrid Lohmann Brown (LB; Lohmann Tierzucht GmbH, Germany) were sampled for allantoic fluid. After the minimally invasive withdrawal the allantoic fluid was analysed via enzyme immunoassays (ELISA) adapted to domestic chicken for 17β-oestradiol (E2), oestrone sulphate (E1S) and testosterone. With regard to E2 and E1S, significant (P < 0.01) sex-specific differences were observed in the allantoic fluid of nine and ten day old embryos. Testosterone on the other hand displayed no gender-related variances on any of the analysed days. Therefore, it proved to be unsuitable for gender identification using the method applied in this study. Statistical analysis showed that the analysis of E1S allows an earlier and more accurate sexing than the E2-assay. The limit value determined for E1S has a sensitivity of 86 % and a specificity of 83 % for nine day old embryos. The previously established method for gender identification via E1S detection in the allantoic fluid was verified with a larger number of samples in the second study. The allantoic fluid of day 8 + 4 h (n = 2420) and day 9 + 4 h (n = 2850) old LB embryos as well as n = 150 day 9 + 4 h old embryos of the white layer-hybrid Lohmann Selected Leghorn (LSL; Lohmann Tierzucht GmbH, Germany) was analysed. For day 8 + 4 h old embryos the sex was correctly identified in 84 %. The accuracy of gender prediction increased for day 9 + 4 h old embryos up to 98 % (LB) and 100 % (LSL). Compared to an untreated control group (n = 5258) sampling of allantoic fluid reduced the hatching rate by 1.4 - 3.5 (LB) and 12.7 points of percentage (LSL). In the following, 150 animals of the experimental group and 80 animals of the control group were reared for a period of 17 weeks. With regard to the body weight significant differences (P < 0.05) were observed in weeks 4 and 6, with the animals of the experimental group having a lower body weight. Subsequently the production performance of 120 hens from the experimental and 60 hens from the control group was analysed up to an age of 33 weeks. With respect to egg production, egg weight, body weight and feed consumption no significant differences (P > 0.05) were observed between the groups. The results of this thesis demonstrate that a reliable in ovo sexing of day 9 + 4 h old chicken embryos is possible via the measurement of E1S in the allantoic fluid; additionally the method is not limited to a certain layer strain. The sampling of allantoic fluid reduces the hatching rate only marginally. The production performance of adult hens on the other hand is not affected. Therefore, the described technique fulfils all the basic requirements for an alternative method to the culling of day-old male layer chicks. Because gender identification takes place before the onset of embryonic pain perception it can serve as the basis for an ethical alternative to the culling of male day-old chicks from layer-hybrids.

В работе проведено исследование, анализ и сравнение различных способов прокладки трубопроводов водоснабжения. Выявлены их особенности, достоинства и недостатки в различных условиях. На основании полученных данных предлагается методика выбора наиболее эффективного способа в зависимости от конкретных факторов. / The study, analysis and comparison of various methods of laying water supply pipelines have been carried out. Their features, advantages and disadvantages in various conditions are revealed. Based on the data obtained, a methodology is proposed for choosing the most effective method, depending on specific factors.

G proteins coupled receptors (GPCRs) play essential developmental roles with functions in all of the immune, olfactory sensory systems amongst other systems as well as exhibiting essential roles in the central and peripheral nervous system. GPCRs are also major targets of pharmaceutical drugs currently used to treat a vast number of conditions. Despite their clear importance, the function of many GPCRs is still obscure. Identifying the physiological role of more GPCRs provides a niche for more drugs to be developed and thus more conditions to be treated. The C.elegans lat-1 gene encodes the latrophilin vertebrate homolog; it is a member of the adhesion GPCR family and is structurally related to the flamingo/CELSR, an essential component of planar cell polarity pathway. This study aims to phenotypically characterise lat-1 mutants in C.elegans to provide insights into the physiological role of this important member of adhesion GPCRs. lat-1 mutants exhibit several morphological defects throughout development and during vulva development. Analysing the embryonic development of such mutants also identified an anterior-posterior polarity defect. The results implicate a second evolutionary conserved subfamily of adhesion GPCRs in the control of tissue polarity and morphogenesis.

Einige Zuchtmerkmale der Honigbiene lassen sich sinnvoll an einzelnen Arbeiterinnen messen. Dabei kann der Zuchtfortschritt potentiell beschleunigt werden, wenn Söhne der selektierten Arbeiterinnen zur Besamung herangezogen werden. Ziel dieser Arbeit war deshalb die Schaffung von Methoden zur Erzeugung von Söhnen selektierter Arbeitsbienen. Um die Fruchtbarkeit der selektierten Tiere zu fördern wurde zunächst vergeblich versucht, eine Hormonbehandlung zur gezielten Induktion der Eibildung zu entwickeln. Dabei konnten die Mechanismen der Fortpflanzungssteuerung bei Arbeitsbienen teilweise aufgeklärt werden. Um die Fruchtbarkeit in allen Bienen außer den selektierten zu unterdrücken wurde zunächst die Sterilisation durch harte Röntgenstrahlung erprobt. Die erhaltenen Tiere legten keine Eier, zeigten aber starke somatische Schäden. Die Aufzucht von Arbeiterinnen-Larven in überalterten Völkern ergab keine sterilen, sondern besonders fruchtbare Tiere. Schließlich gelang es unter Verwendung von Flugbienen als Begleittieren, in Jungbienen in über 60% der Fälle volle Ovarentwicklung zu erzielen. Auch konnten alle erzeugten Eier Jungbienen zugeordnet werden. Um die Eignung von Arbeiterinnen-gelegten Eiern für Zuchtzwecke zu prüfen, wurden Qualitätsparameter gemessen. Arbeiterinnen-gelegte Eier zeigten sich empfindlicher gegenüber Trockenheit und mechanischer Beschädigung als Königinnen-gelegte Eier. Alle in Völker verbrachten Arbeiterinnen-gelegten Eier wurden ausgefressen. Also wurde eine Methode zur Anfütterung von Larven im Brutschrank entwickelt. Die so erzeugten Larven überlebten aber ebenfalls nicht in Völkern. Deshalb wurde ein Verfahren entwickelt, mit dem aus über 50% der eingesetzten Larven durch Brutschrankaufzucht adulte Tiere erhalten werden können. Ein vorläufiges Protokoll zur Erzeugung von Söhnen individuell selektierter Arbeitsbienen wurde erarbeitet. / Breeding of honey bees usually involves selection at the colony level. However, some important breeding traits exist that can also be measured at the scale of the individual worker bee. In these cases, genetic progress can potentially be accelerated by using drones which are sons of the selected individuals. The aim of this study was therefore to create methods for the production of offspring from individually selected workers. To enhance fertility of selected workers, an attempt was made to develop a hormonal treatment to induce worker fertility. It led to the partial elucidation of the endocrine regulation of reproduction in worker honey bees. Methods were tested to suppress fertility in all bees except the selected workers, in order to prevent competition among workers and enable identification of the eggs of selected bees. Method tested included the application of ultra-hard X-rays and rearing of larvae inside over-aged colonies. Finally, it could be shown that forager bees can be used as companion bees to selected workers. This method led to fully developed ovaries in more than 60% of bees representing the selected workers in these tests, who also were the only animals to produce eggs. The next subtask was the measurement of characteristics linked to viability in eggs from laying workers. Worker-laid eggs showed to be more sensitive to dryness and mechanical damage. When worker-laid eggs were inserted into queenless or queenright colonies for rearing, they were systematically cannibalised. Therefore, a method was developed to rear eggs into living larvae in an incubator. However, the larvae produced in this way were not accepted by colonies either. To circumvent this problem, a protocol was designed and validated that allows to rear worker-derived drone larvae into adults in vitro. The study led to a preliminary protocol for the production of drones that are sons of individually selected worker bees.

Esta tese aborda o processo de formação territorial e de crescimento econômico de Santa Maria de Jetibá, um município do Espírito Santo. Objetiva-se, então, compreender o processo de transformação de Santa Maria de Jetibá, que vai de um longo período de penúria dos agricultores ao crescimento econômico recente. Produzindo alimentos, o município conquistou o maior valor adicionado da agropecuária do Espírito Santo e destaca-se como o segundo maior produtor de ovos do país e no âmbito estadual como produtor de alimentos orgânicos e hortaliças. Buscou-se identificar as condicionantes materiais e imateriais que foram determinantes no processo de formação e transformação desse território e que explicam o seu crescimento econômico. O trabalho de campo foi organizado em duas partes. Na primeira, tomando como referência o processo de trabalho, segundo Marx, e suas alterações decorrentes da incorporação de tecnologias, foram elaborados roteiros de entrevistas. Entrevistou-se agricultores familiares e não familiares e diferentes tipos de avicultores. Na segunda, para entender a estruturação das principais atividades econômica, entrevistou-se representantes de instituições pertinentes ao objetivo da tese. Identificou-se como condicionante imaterial a dimensão ética do trabalho dos pomeranos e seus descendentes, os quais sempre apresentaram severa disciplina para o trabalho que os diferenciava dos demais imigrantes europeus. Tal ética tornou-se uma qualidade desse grupo humano que por ser proveniente de uma província onde não teve acesso a propriedade da terra e por não ter uma nação que o representasse, encontrou na migração para esse território uma possibilidade de crescer economicamente por meio do trabalho. Além disso, a experiência com atividades comerciais de um determinado grupo de avicultores, contribuiu para a estruturação de uma cadeia produtiva com elevado nível de verticalização, que conferiu caráter competitivo à atividade, mesmo em um contexto produtivo desfavorável. Como condicionantes materiais destacam-se: a construção de estradas interligando Santa Maria de Jetibá a importantes centros consumidores e a introdução e ampliação da rede de energia elétrica na zona rural, duas condições gerais de produção fundamentais para as atividades agropecuárias; a modernização da agricultura, que disponibilizou crédito, insumos e meios de produção modernos; a evolução constante das inovações tecnológicas voltadas para a agropecuária, sempre ampliando a produtividade e reduzindo o tempo de produção; a ampliação do mercado consumidor em função da industrialização e da urbanização do estado; a abertura do mercado que permitiu importar as inovações que automatizaram a avicultura de postura; as condições naturais favoráveis à produção de hortaliças e de ovos e a proximidade de grandes centros consumidores. Constatou-se também, que existem importantes diferenças tecnológicas entre a avicultura de postura e a produção de hortaliças convencionais e orgânicas. Enquanto na primeira, constituída somente por um produto, grande parte das barreiras impostas pela natureza à reprodução do capital foi eliminada pelas inovações técnicas, na segunda, constituída por inúmeras variedades de cultivos, muitas barreiras naturais ainda permanecem. Assim, na avicultura de postura predominam granjas de porte grande e o trabalho assalariado, enquanto a produção de hortaliças ocorre em pequenas propriedades e assentada no trabalho familiar. / These thesis approach the process of territorial formation and grow of Santa Maria De Jetiba a town in the State of Espirito Santo. Hence the goal is to understand the process of transformation of Santa Maria de Jetiba from a long period of hardship to the recently farmers economic grow. Producing food, the town conquer a high value of agriculture in the State and stands out as a second major on eggs production, also producing organic food and vegetables in the State. The identification of material and non-material elements, it was crucial for the process formation of the territory and transformation, explaining the economic growth. The fieldwork it was organized in to two parts. The fist part taking reference on the working process by Marx,and alterations as a result of the technology introduction, with elaboration of interview to farmers family members and non family members also to poultry farmers.The second part was to understand about the structure of the major economic activities, representatives of institutions related to the thesis goal. It was identified the ethical work dimension of the pomeranos and descendants, always demonstrating severe work discipline that made them standout from others european imigrants. That ethic it was a quality, despite the fact that they did not have land access and for not having a nation represented. The migration to this territory it was a possibility of economic grow through work. In addition the poultry farmers experience for commercial activities, contributed with a structure for a production chain and high level of verticality given a competitive character to the activities. Even without a favorable production context. For instance some material elements should be consider: road construction connecting Santa Maria De Jetiba to consumers majors centers ,implantation and extending of power lines in rural district are key conditions for production and agriculture, credit availability, components and modern production, constant technology evolution, innovation in agriculture, increasing productivity and decreasing production time, growth of consumers as a result from the State industrialization and urbanization, the open market that brings innovations to the automation in poultry laying, favorable natural conditions for vegetables and eggs production, close to major consumers centers. It was possible to noted the difference in technology from poultry laying and conventional vegetables and organics production. The fist constitute only by one product, the technical innovations eliminates natural barriers. On the second, because of variety of cultures, natural barriers still present. Therefore, in poultry laying the farms are predominantly bigger, the workers get paid a fixed amount of money monthly, in vegetables production the farms are smaller and the work are made by the family.

O objetivo geral desta pesquisa foi avaliar o bem-estar de aves poedeiras em dois diferentes sistemas de criação (gaiola e cama) e a qualidade desses sistemas em relação aos aspectos comportamentais, fisiológicos e produtivos utilizando ferramentas da zootecnia de precisão. O experimento foi desenvolvido no Setor de Avicultura do Departamento de Zootecnia da Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP) em Piracicaba, SP. A pesquisa constou de cinco períodos experimentais de 28 dias cada. Foram utilizadas 64 poedeiras da linhagem Isabrown e 64 poedeiras da linhagem Hy-Line W-36 com 19 e 21 semanas de idade respectivamente. O regime de luz adotado foi de 16 horas claro e 8 horas de escuro. Foram estabelecidos 2 tratamentos, sendo Tratamento 1: criação em gaiolas convencionais; Tratamento 2: sistema de criação em cama. O delineamento experimental adotado foi o inteiramente casualizado, em esquema fatorial 2x2 (2 linhagens e 2 sistemas de criação) com 4 repetições cada. A pesquisa abordou diferentes parâmetros: análise bioclimática; análise fisiológica; análise do desempenho zootécnico; análise da qualidade dos ovos; análise dos comportamentos; análise das condições de empenamento e aplicação da lógica Fuzzy na avaliação dos dados. A avaliação térmica foi realizada mediante a análise dos dados de temperatura do ar seco, da temperatura de globo negro e da umidade relativa, utilizando os índices de Temperatura de Globo e Umidade (ITGU) e Entalpia Específica (H) (KJ/kg ar seco). A análise das condições meteorológicas mostrou que a maior parte dos períodos experimentais ocorreu em condições de conforto térmico para as aves. A avaliação do comportamento permitiu identificar atividades de preferência e atividades indicativas de estresse, possibilitando a determinação das necessidades ambientais importantes ao bem-estar das aves. A avaliação comportamental por meio de imagens de vídeo permitiu uma análise mais apurada, fornecendo um banco de dados mais confiável do que a observação direta. O sistema de criação em cama, quando devidamente projetado, pode ser compatível ao sistema de criação em gaiolas no que diz respeito ao desempenho zootécnico e à qualidade de ovos produzidos; além disso, mostrou estimular a realização dos comportamentos naturais das aves e dessa forma, maior adequação às exigências de bem-estar. Porém, a melhor condição de bem-estar do sistema de criação em cama não apresentou melhor resultado no desempenho produtivo. A aplicação da lógica Fuzzy na obtenção de parâmetros indicativos das condições de bem-estar das aves nos diferentes sistemas de criação forneceu um modelo de fácil interpretação, permitindo a integração dos parâmetros avaliados, tornando-se uma ferramenta importante na interpretação das condições de bem-estar das aves. / The objective of this study was to evaluate the welfare of laying hens in two different rearing systems (cages and floor) and the quality of these systems concerning with the behavioural, physiological and productive aspects, using tools of precision animal production. The trial was conducted for five experimental periods of 28 days each. Sixty four pullets of the laying strain Isabrown and 64 pullets of the laying strain Hy-Line W-36, with ages of 19 and 21 weeks, respectively, were used. The treatments consisted of a combination of two rearing systems (conventional cages system and floor system with litter) and the two layer strains in a completely randomized design, with a 2x2 factorial arrangement, and four replicates. Different evaluation parameters were studied including bioclimatical; physiological; productive performance; behavioural; egg quality; feathering conditions and application of Fuzzy logic in data evaluations. The thermal evaluation was carried by analysis of dry air and black globe temperature and relative humidity, using the indices Temperature of Globe and Humidity (ITGU) and Enthalpy (H) (KJ/kg dry air). The analysis of the meteorological conditions showed that most of the experimental periods occurred in conditions of thermal comfort for the birds. The evaluation of the bird&#39;s behaviour allowed identifying preference activities and those indicative of stress, making possible the determination of bird&#39;s environmental necessities, important to their welfare. The behavioral evaluation through video images allowed more refined analysis, supplying a more trustful data base than direct observations. The floor system, when properly projected, can be comparable to the cage system with respect to the productive performance and the egg quality; moreover, it was shown to stimulate the accomplishment of natural behaviours, thus providing greater adequacy to the welfare requirements. However, the improved welfare conditions resulting of the floor system did not produce better results in the productive performance. The application of Fuzzy logic in the attainment of indicative parameters of the conditions of laying hens welfare in different rearing systems supplied an easy model, allowing the integration of the evaluated parameters, becoming an important tool in the interpretation of welfare conditions.