El conocimiento de la estructura de las proteínas constituye una de las principales aproximaciones hacia el entendimiento de las bases moleculares de las enfermedades humanas; en particular, puede ser usada para racionalizar los efectos de muchas mutaciones causantes de enfermedades, que a menudo son asociadas con cambios en la estabilidad de las proteínas. En este trabajo, se estudia el Dominio BRCT, módulo evolutivo de aproximadamente 95 aminoácidos, con plegamiento autónomo, conservado a través de la mayoría de taxas y encontrado en muchas proteínas relacionadas a la reparación del DNA, recombinación y control del ciclo celular. Una de las características conservadas del plegamiento del dominio BRCT es la estructura formada por dos alfa hélices en una región de la molécula, haciendo frente a la hoja plegada ß central, que contiene dos de las mas conocidas mutaciones relacionadas a cáncer de mama, y que resultan en la introducción de un residuo cargado que desestabiliza la interfase del complejo BRCT-BRCT en la proteína BRCA1. Para este trabajo, elegimos probar la estabilidad del BRCT presente en la enzima DNLJ ligasa de una bacteria termófila mediante la remoción de la carga de superficie, “R21”, de esta estructura. Este es un primer paso para evaluar si la carga de superficie contribuye significativamente a la estabilidad de este dominio termofílico, para profundizar en el posible rol de la estabilidad del BRCT en el desarrollo del cáncer de mama, y por encima de todo, para determinar las bases de la estabilidad de este dominio que dada su frecuencia, se constituye en un hecho biológicamente relevante. Los resultados preliminares sugieren que la estabilidad del dominio BRCT de la DNLJ ligasa de Thermus thermophilus, es tolerante a la remoción de cargas en su superficie, pero es afectado por las variaciones en la hidrofobicidad. Aunque estas mutaciones no muestran variaciones significativas en la estructura y la estabilidad del dominio, si podrían estar afectando la habilidad de interactuar con otras proteínas. / The knowledge of protein structure constitutes one of the main approaches towards understanding the molecular basis of human disease, in particular, protein structure can be used to rationalize the effects of many disease-causing mutations, which often are associated with changes of protein stability. In this work, the BRCT domain is studied, an evolutionary protein module with autonomous folding, of approximately 95 aminoacids, found in numerous proteins involved in DNA repair, recombination and cell cycle control. One of the conserved features of the BRCT fold is a two helical bundle located against one side of the central four-stranded ß sheet, which features two of the best known mutations in breast cancer resulting from introducing a distabilizing charge within the interface of the BRCT-BRCT complex in BRCA1. We have chosen to probe the stability of the BRCT of DNLJ ligase in a thermophilic bacteria by removing the surface charge "R21" from this bundle. This is a first step to test whether this surface charge contributes significantly to the stability of this thermophilic domain, to gain insight about the possible role of BRCT stability on the breast cancer disease, and most of all, to determine the basis for the stability of this frequently found and biologically relevant structural domain. Our preliminar results suggest that the stability of the BRCT of the DNLJ ligasa of Thermus thermophilus BRCT domain is tolerant to the surface charge removal, but is affected by hydrophobicity. Although these mutations do not show significant variations in the structure and stability of the domain itself, they may affect their ability to interact with other protein modules.

El cáncer de mama es frecuente en la mujer limeña y ocupa el segundo a nivel nacional, por ello el autoexamen de mama se constituye una medida de prevención, importante para detectar alguna anormalidad. Este estudio tuvo el objetivo de determinar los conocimientos, actitudes y prácticas del autoexamen de mama en estudiantes de enfermería de la Universidad Nacional Mayor de San Marcos (UNMSM). Es de tipo cuantitativo, nivel aplicativo, método descriptivo y de corte transversal; la muestra fue seleccionada a través de un muestreo probabilístico, estratificado; el tamaño de la muestra fue de 123 estudiantes del sexo femenino, se obtuvo con la fórmula de “Determinación del tamaño de muestra para estimar una proporción”. La técnica que se utilizó fue la entrevista, y como instrumentos, un cuestionario y escala modificada tipo Lickert. Los resultados fueron, los estudiantes del 1° año de estudios, tienen conocimiento bajo el 72 %. De 2º a 5º año, predomina el conocimiento medio con más del 50 % en cada año. En actitudes, todos los años presentaron actitud de aceptación abarcando más del 55 % de la muestra en cada año. Mas del 40 % de cada año no practica la técnica del autoexamen de mama. La conclusión fue la mayoría de estudiantes de Enfermería de la UNMSM poseen conocimiento medio y su tendencia es aceptación hacia el autoexamen de mama, sin embargo la mayoría no realiza la técnica del autoexamen de mama. -- Palabras claves: autoexamen de mama, estudiantes de enfermería, conocimientos, actitudes, prácticas. / -- Breast cancer is common in Lima women and ranks second nationally, so the breast self-examination is a preventive measure, important to detect any abnormalities. This study aimed to determine knowledge, attitudes and practices of breast self-examination nursing students from the Universidad Nacional Mayor de San Marcos (UNMSM). Is quantitative, application level, descriptive method and crosssectional, the sample was selected through a probabilistic, stratified, the sample size was 123 female students, was obtained with the formula of "Determination of sample size for estimating a proportion. "The technique used was the interview, and as an instrument, a questionnaire and Lickert scale. The results were, students from 1 year of study, have knowledge under 72%. From 2 to 5th year, the average knowledge predominates over 50% each year. Attitudes, all the years presented more accepting attitude comprising 55% of the sample in each year. Over 40% of each year did not practice breast self-exam technique. The conclusion was that most nursing students of San Marcos have average knowledge and acceptance trend toward breast self-examination, however, most art does not perform breast selfexamination. -- Keywords: breast self-examination, nursing students, knowledge, attitudes, practices.

Se presenta la evaluación de la utilización clínica del Colgajo Miocutáneo Transverso de Recto Abdominal realizado en el Servicio de Cirugía Plástica y Quemados del Hospital Central de la Policía Nacional del Perú – Lima entre Febrero de 1999 y Diciembre del 2001, para la reconstrucción de la glándula mamaria. Se evaluaron siete pacientes en quienes se realizó esta técnica siendo los resultados obtenidos muy favorables. Se requirió un solo tiempo operatorio, sin necesidad de técnica microquirúrgica, la elevación del colgajo fue relativamente sencilla, rápida y segura, obteniéndose resultados satisfactorios, aún cuando se requiera posteriormente un refinamiento estético. Se empleó el Colgajo TRAM en seis casos con antecedentes de adenocarcinoma mamario ductal infiltrante y en una paciente portadora de Síndrome de Poland, recomendándose su uso en la reconstrucción mamaria por los resultados obtenidos.

Orientador: Gilberto Uemura / Banca: Maria de Fátima Brito Vogt / Banca: Eduardo Carvalho Pessoa / Resumo: Objetivo: Determinar o valor preditivo positivo para malignidade de lesões mamárias classificadas nas subcategorias 4A, 4B e 4C do sistema BI-RADS® pela correlação entre os achados mamográficos e histopatológicos. Sujeitos e métodos: Estudo analítico-descritivo de 89 pacientes submetidas à core biopsy e biópsia cirúrgica com e sem estereotaxia, que possuíam mamografias classificadas nas subcategorias 4A, 4B e 4C do BI-RADS® 4ª edição. Os achados mamográficos foram correlacionados com os diagnósticos histológicos das lesões, calculando-se o valor preditivo positivo para malignidade em cada subcategoria. Resultados: Dentre as 89 pacientes avaliadas foram diagnosticados 50 casos de câncer de mama (56,2%), sendo 9 na subcategoria 4A, 21 na 4B e 20 na 4C. Os valores preditivos positivos encontrados foram, respectivamente, 25,7 %, 63,6 % e 95,2 % para as subcategorias 4A, B e C. O nódulo espiculado foi o achado mais relacionado à doença maligna. Conclusão: Houve elevação progressiva do VPP nas subcategorias 4A, 4B e 4C do BIRADS ® / Abstract: Objective: To determine the positive predictive value for malignancy of mammary lesions classified in the subcategories 4A, 4B and 4C of the BI-RADS® system based on the correlation between the mammographic and histopathological findings. Subjects and methods: Analytical-descriptive study of 89 patients submittted to core biopsy and surgical biopsy with and without stereotatic localization, that possessed mammograms classified as subcategories 4A, 4B and 4C of the BI-RADS® 4th edition. The mammographic findings were correlated to the histological diagnosis of the lesions, then calculating the positive predictive value for malignancy in each subcategory. Results: Among the 89 evaluated patients, 50 were diagnosed as breast cancer cases (56.2%), of which 9 in subcategory 4A, 21 in 4B and 20 in 4C. The positive predictive values found were, respectively, 25.7%, 63.6% and 95,2% for the 4A, B and C subcategories. The spiculated nodule was the finding most related to the malignant disease. Conclusion: There has been progressive elevation of the PPV in subcategories 4A, 4B and 4C of BI-RADS® / Mestre

Orientadores: Luiz Carlos Teixeira, Marcelo Alvarenga / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas / Made available in DSpace on 2018-09-11T21:07:48Z (GMT). No. of bitstreams: 1 Manna_ElizaDelFiol_M.pdf: 877307 bytes, checksum: 7ee78365634d93a3ce0c44565891f5ee (MD5) Previous issue date: 2005 / Resumo: Objetivo: O objetivo deste estudo foi avaliar a associação entre a expressão imunoistoquímica da topoisomerase IIa, HER2 e receptores hormonais e a resposta à quimioterapia primária baseada em antraciclina em carcinoma invasivo de mama. Materiais e Métodos: Analisamos 109 prontuários de pacientes tratadas com quimioterapia primária baseada em antraciclina no Centro Atenção Integral à Saúde da Mulher da Universidade Estadual de Campinas, no período de 1996 a 2004. As respostas clínica e patológica à quimioterapia primária foram associadas com a superexpressão da topoisomerase IIa e do HER2 e com a negatividade dos receptores hormonais. A análise estatística foi realizada através do teste qui-quadrado ou teste exato de Fisher. Resultados: A freqüência da superexpressão da topoisomerase IIa foi de 41%. Não houve associação estatística entre a resposta clínica e a superexpressão da topoisomerase IIa, do HER2 e negatividade dos receptores hormonais. Entretanto, houve associação entre a resposta completa patológica e a negatividade dos receptores hormonais (p=0,0289). Conclusões: O presente estudo sugere que esses marcadores não deveriam ser considerados fatores preditivos de resposta à quimioterapia primária com antraciclina, sendo necessários estudos prospectivos desenhados para esse propósito / Abstract: Background: The aim of this study was to evaluate the association between immunohistochemical expression of topoisomerase IIa, HER2 and hormonal receptors and response to primary anthracyclin-based chemotherapy in invasive breast carcinoma. Materials and Methods: We analyzed 109 medical charts of patients treated with primary anthracyclin-based chemotherapy in Women¿s Integral Health Care Center of State University of Campinas from 1996 to 2004. The clinical and pathological response to primary chemotherapy was associated with overexpression of topoisomerase IIa and HER2 and hormonal receptor negativity. Statistical analysis was performed using Chi-square or Fisher¿s Exact Test. Results: The frequency of topoisomerase IIa overexpression was 41%. No statistical association between clinical response and overexpression of topoisomerase IIa, HER2 and hormonal receptor negativity was found. However, there was an association between complete pathological response and hormonal receptor negativity (p=0.0289). Conclusions: The present study suggested that these markers should not be considered predictors of response to primary anthracyclin-based chemotherapy, and prospective studies must be designed for this purpose / Mestrado / Ciencias Biomedicas / Mestre em Tocoginecologia

Se propone conocer la relación entre las estrategias de afrontamiento y el apoyo social percibido en la etapa de diagnóstico reciente. La muestra estuvo conformada por 56 mujeres con cáncer de mama, cuyas edades fluctúan entre 27 y 80 años (M=53.71, DE=12.87). El tiempo de diagnóstico oscila entre 1 y 8 meses y fueron atendidas en un centro de prevención en Lima Metropolitana. Se empleó el Cuestionario de Estimación del Afrontamiento (COPE) (Crespo & Cruzado, 1997) y el cuestionario MOS de Apoyo Social en Atención Primaria (De la Revilla et al., 2005). Los resultados evidencian relaciones significativas y directas entre los índices de apoyo percibido y las estrategias: reinterpretación positiva, planificación, acudir a la religión; uso del humor y, una relación inversa con negación. Apoyo emocional/informacional y apoyo afectivo se relacionan directamente con las estrategias búsqueda de apoyo por razones emocionales y aceptación. El apoyo afectivo se relaciona con búsqueda de apoyo instrumental. La edad se relaciona positivamente con acudir a la religión y el apoyo instrumental; y, tener pareja presenta una relación positiva con apoyo afectivo. Se concluye que, en la fase de diagnóstico reciente, se evidencia una relación entre el apoyo social percibido y las estrategias centradas en la emoción; las pacientes hacen uso de diversas estrategias; y, que el apoyo social incide en el uso de estrategias consideradas como adaptativas. Esta información podría ser usada en propuestas de intervención en el diagnóstico reciente con fin de lograr un mejor ajuste al cáncer. / The aim of this paper was to explore the relationship between the coping strategies and the perceived social support in the recent diagnosis stage. In a group of 56 women with breast cancer, whose ages fluctuate between 27 and 80 (M=53.71, DE= 12.87). The diagnosis time ranges from 1 to 8 months and they were treated in a prevention centre in Lima. The Questionnaire for Estimating Coping (COPE) (Crespo & Cruzado, 1997) and the MOS Questionnaire for Social Support in Primary Care (De la Revilla et al., 2005) were used. The results evidence the significant and direct relationships between the perceived support indices and the strategies: positive reinterpretation, planning, becoming religious; humor using and an inverse relationship with denial. Emotional/Informational support and affective support are directly related to the strategies search for support for emotional reasons and acceptance. Affective support is directly related to the strategies search for support for instrumental reasons. Age is positively related to becoming religious and the instrumental support; and, having a partner has a positive relationship with affective support. In conclusion: in the recent diagnosis phase, there is a relationship between perceived social support and strategies focused on emotion; the patients make use of different strategies; and, social support affects the use of strategies considered as adaptive. This information could be used in intervention for the recently diagnosis in order to achieve a better adjustment to cancer. / Tesis

El cáncer de mama puede ser considerado un evento de vida potencialmente estresante pues demanda cambios que tienden a exceder la capacidad de las pacientes para afrontar dicha condición, lo que afecta negativamente su CVRS. Este estudio examina la contribución que tienen el estrés percibido, el estrés traumático, los estilos de afrontamiento, la migración y el tipo de tratamiento recibido sobre la variabilidad de los componentes físico y mental de la CVRS de 53 mujeres mastectomizadas, que han sido diagnosticadas por primera vez con cáncer de mama. Los instrumentos utilizados comprendieron la adaptación española del cuestionario de salud (SF-36) (Alonso, Prieto & Antó, 1995), el inventario de estimación de afrontamiento COPE (Carver, Scheier & Weintraub, 1989), la escala de estrés percibido EEP (Cohen, Kamarck & Mermelstein, 1983) y la adaptación peruana de la escala de estrés traumático (Gargurevich, 2006). Se encontraron niveles promedio y altos de la CVRS. El componente físico se vio más afectado que el componente mental, especialmente en las pacientes migrantes. En el componente físico, se obtuvo un modelo conformado por la migración, el tipo de tratamiento y el estrés traumático, quienes explicaron el 30% de su variabilidad. En tanto, en el componente mental se obtuvieron dos modelos; el primero conformado por el estrés traumático y el estrés percibido, quienes explicaron el 39% de su variabilidad; mientras que el segundo, conformado por el estrés traumático, estrés percibido y el estilo orientado a la emoción explicaron el 42% de su variabilidad. Palabras clave: Calidad de vida relacionada a la salud (CVRS), afrontamiento, estrés percibido,estrés traumático, cáncer de mama, migración y tipo de tratamiento. / Breast cancer might be considered a life-threatening event as it demands changes that could exceed the patient’s abilities to cope with their illness, therefore, affect HRQoL in a negative way. This study analyzes the contribution that traumatic stress, perceived stress, coping, migration and type of treatment have on the variability of physical and metal components of the HRQoL of 53 mastectomized women with breast cancer. The instruments used include the Spanish adaptation of the SF-36 health survey (Alonso, Prieto & Antó, 1995), the COPE Inventory (Carver, Scheier & Weintraub, 1989), the Perceived Stress Scale PSS (Cohen, Kamarck & Mermelstein, 1983) and the Peruvian adaptation of the traumatic stress scale (Gargurevich, 2006). The analysis found medium and high levels of the HRQoL. Physical component was more affected than mental component, especially in migrant patients. The model of the physical component was comprised by migration, treatment type and traumatic stress, which explained 30% of its variability. On the other hand, two models were obtained from the mental component; the first was comprised by traumatic stress and perceived stress, which explained 39% of its variability, while the second model, comprised by traumatic stress, perceived stress an emotion-oriented stress, explained 42% of its variability / Tesis

La presente investigación tiene como objetivo general describir y comparar el bienestar psicológico y sus dimensiones en mujeres con cáncer de mama sometidas a tratamiento quirúrgico frente a mujeres sanas que comparten sus características sociodemográficas. Se evaluó una muestra de 28 pacientes con cáncer de mama, entre los 36 y 75 años (M=56,11, DE=11.93), que acuden a consulta externa en una institución médica del estado. Se utilizó la adaptación de Díaz et al. (2006) de la Escala de Bienestar Psicológico de Carol Ryff (1989), la cual cuenta con seis dimensiones: autoaceptación, propósito en la vida, dominio del entorno, crecimiento personal, autonomía y relaciones positivas con otros. No se encontraron diferencias significativas entre ambos grupos a nivel de la escala global de bienestar psicológico y por dimensiones. En respuesta a los objetivos específicos, se encontró correlación positiva entre la edad y las dimensiones de autonomía y autoaceptación. Asimismo, se obtuvo correlación negativa entre la variable número de cirugías realizadas y la medida de bienestar psicológico global, además de las dimensiones autoaceptación, propósito en la vida y autonomía. En el caso de la variable tipo de cirugía realizada, se obtuvo diferencias significativas entre las pacientes que debieron someterse únicamente a una tumorectomía y aquellas que debieron afrontar la tumorectomía y la mastectomía radical. Fueron las primeras quienes presentar mayores niveles de bienestar a nivel global y en las dimensiones de autoceptación y autonomía. / The objective of this investigation is to describe and compare the construct known as psychological well-being and its dimensions in women with breast cancer who had surgery in contrast to healthy women who share the same demographic characteristics. The sample consisted of 28 breast cancer patients, between the ages of 36 and 75 years old (M=56.11, SD= 11.93). The instrument used was the adaptation made by Diaz et al. (2006) of Ryff's Psychological Well- Being Scales (1988), which dimensions are: self-acceptance, autonomy, purpose in life, environmental mastery, personal growth and positive relations with others. No significant differences were found between both groups at the level of the global scale of psychological well-being and its dimensions. In response to the specific objectives, we found positive correlation between age and the dimensions of autonomy and self-acceptance. Negative correlation was found between the variable "number of surgeries" and global psychological well-being, self-acceptance, purpose in life and autonomy. The variable "type of surgery" presented significant differences between the group of patients that had a tumorectomy only and the group that went under two surgeries (tumorectomy and mastectomy). The first group reported higher levels of global psychological well-being, autonomy and self-acceptance. / Tesis

Orientador: Debora Aparecida Pires de Campos Zucari / Banca: Paula Rahal / Banca: Alessandra Vidotto / Resumo: As neoplasias mamárias são os tumores mais comuns nas mulheres e fêmeas caninas. Fibroblastos associados ao câncer (CAFs) são células abundantes no microambiente tumoral, capazes de intermediar respostas inflamatórias atuando na diferenciação e progressão tumoral. A melatonina está associada a mecanismos de ação com efeitos oncostáticos e oncoprotetores, e atua na redução da formação de fibroblastos circundantes no câncer de mama. Os objetivos desse estudo foram verificar a ação da melatonina nas linhagens tumorais mamárias metastáticas humana e canina (MDA-MB-231 e CF-41) bem como em co-cultivo com CAFs primários na viabilidade celular e na expressão de proteínas envolvidas nos processos de angiogênese, inflamação e apoptose, buscando o entendimento da interação destas proteínas nas principais vias de sinalização atuantes no processo tumorigênico. A viabilidade celular foi verificada pelo ensaio MTT e a expressão proteica pelo Membrane Antibody Array após o tratamento com melatonina. Verificou-se que 1 mM de melatonina reduziu a viabilidade celular em ambas as linhagens, CAFs humanos e caninos e em seus co-cultivos (p < 0,05). A análise proteica semiquantitativa, após tratamento com melatonina, mostrou redução da expressão de sete proteínas pró-angiogênicas e aumento das proteínas TIMP-1 e IL-6 nas células MDA-MB-231 e redução de IL-6 e MCP-1 e aumento da leptina, TGF-β, trombopoietina e VEGF na co-cultura (p < 0,05). Houve redução de dezenove proteínas pró-inflamatórias e aumento da citocina I-309 nas células MDA-MB-231 (p < 0,05). No co-cultivo, seis proteínas pró-inflamatórias apresentaram-se menos expressas e as proteínas IL-4, MCP-3 e SCF aumentaram sua expressão (p < 0,05). Das proteínas pró-apoptóticas, doze aumentaram a expressão e houve redução da caspase-3 nas células MDA-MB-231 (p < 0,05). Na co-cultura, houve aumento das proteínas BIM, HPS27 e IGF-1R e redução de... / Abstract: Mammary tumors are the most common tumors among women and female dogs. Fibroblasts associated with cancer (CAFS) are the most abundant cells in the tumor microenvironment, able to mediate inflammatory responses acting in the differentiation and tumor progression. Melatonin is associated with mechanisms of action with oncostatics effects and oncoprotectors, and works to reduce the formation of surrounding fibroblasts in breast cancer. The objectives of this study were to assess the action of melatonin on mammary tumor cell lines human and canine metastatic (MDA-MB-231 and CF-41) and in co-culture with primary CAFS on cell viability and expression of proteins involved in the processes of angiogenesis, inflammation and apoptosis searching for the understanding of proteins interaction in the main signaling pathways active in the tumorigenic process. Cell viability was assessed by MTT assay and the semi-quantitative protein analysis by Membrane Antibody Array after treatment with melatonin. It was found that 1 mM of Melatonin reduced cell viability in both cell lines, human and canine CAFS and their co-culture (p <0.05). The semi-quantitative protein analysis after melatonin treatment showed a reduction of the expression of seven pro-angiogenic proteins and increased TIMP-1 and IL-6 protein in MDA-MB-231 cells and reduced IL-6 and MCP-1 and increased leptin, TGF-β, VEGF and thrombopoietin in the co-culture (p <0.05). Nineteen pro-inflammatory cytokine protein were reduced and I-309 increased in MDA-MB-231 cells (p <0.05). In the co-culture, six pro-inflammatory proteins were underexpressed and the IL-4, MCP-3 and SCF proteins were increased the expression (p <0.05). Twelve pro-apoptotic proteins increased and decreased expression of caspase-3 in MDA-MB-231 cells (p <0.05). In the co-culture, an increase of BIM, HPS27 and IGF-1R proteins and TNF-RII reduction was observed (p <0.05)... / Mestre

Orientador: Wilter Ricardo Russiano Vicente / Banca: Maricy Apparicio Ferreira / Banca: Marcela Marcondes Pinto Rodrigues / Resumo: O estudo dos tumores mamários em cadelas revela-se como um excelente modelo para a investigação das neoplasias mamárias em mulheres e tanto no homem quanto nos animais a resposta imune pode interferir no desenvolvimento de neoplasias. Este trabalho teve como objetivo avaliar a eficácia e estimulação do sistema imune celular em tumores mamários de fêmeas caninas por meio da expressão de CD4, CD8, CD4+CD25+ (Foxp3), CD28, CD152 e CD56 (NK). A avaliação da expressão desses marcadores foi avaliada por imunoistoquimica, utilizando-se o método estreptoavidina-biotina-peroxidase. Para a realização desse estudo foram coletadas 20 amostras de tumores mamários de fêmeas caninas e essas divididas de acordo com a classificação histopatológica em 2 grupos: 10 carcinomas simples e 10 carcinomas complexos. Em relação aos resultados não se observou diferenças quanto à quantificação das células imunomarcadas quando comparadas segundo o tipo tumoral. No caso dos carcinomas simples evidenciou-se diferença entre a quantificação das células imunomarcadas (P< 0,05) pelo anticorpo CD28 (p=0,03) e Foxp3 (p=0,01) sendo menores que todas as demais marcações. Já para os carcinomas complexos houve diferença entre a quantificação das células imunomarcadas (P= 0,05) pelo anticorpo CD-152 que apresentou valor maior que as demais marcações com exceção do CD8. Os marcadores CD28, CD56 e Foxp3 foram menores que o CD8 (p=0,01) sendo o Foxp3 menor que todos os outros marcadores. No presente estudo observou-se que existe um controle negativo do sistema imune em ambos os casos, porém esse controle negativo foi mais evidente no carcinoma complexo / Abstract: The mammary tumor study in female dogs is revealed as an excellent model for the investigation of breast tumors in humans. On both species, the immune system can interfere on neoplasia progression. The aim of this study was to evaluate the immunolocalization and quantification of CD4, CD8, CD4+CD25+ (Foxp3), CD28, CD152 and NK in tumour lymphocyte infiltration, to evaluate the efficacy of stimulation and immune system defense in canine mammary carcinoma. The expression of these markers using immunohistochemistry was made by streptoavidin-biotin peroxidase method. The tissues were collected from twenty mammary carcinomas, subdivided in ten simple carcinomas and ten complex carcinomas of twenty female dogs. This study identified no significant differences on quantification of positive cellular staining in simple carcinoma compared with complex carcinoma. The samples of simple carcinoma resulted significant differences on positive staining between the antibodies CD28 (p=0,03) and Foxp3 (p=0,01) and these markers revealed less quantitative staining compared with the others markers (CD4, CD8, CD152 and NK). The samples of complex carcinoma resulted significant differences on cellular staining quantification by the antibody CD152 (p<0,05) compared with the others markers (CD4, Foxp3, CD28, and NK) with exception when compared with CD8. The immune quantification of positive cellular staining about the markers CD28, CD56 and Foxp3 were smaller than CD8 (p=0,01), and the Foxp3 was smallest than those others markers. It was concluded that exist a negative control of immune system to complex and simple mammary carcinoma, but, this negative control was more significant in female dogs with complex mammary carcinoma / Mestre