Made available in DSpace on 2016-04-28T14:56:36Z (GMT). No. of bitstreams: 1 2009 - Regina Paula Willemen Pereira 1.pdf: 774444 bytes, checksum: 99a882606ae06eb557016e70b285c10d (MD5) Previous issue date: 2009-05-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The main objective of this research was to work out models for the study of the lignification. Eucalyptus grandis seedlings were produced in vitro, and the stem segments explants were used to obtain friable callus. For such, the auxins tested were TDZ, 2,4-D, NAA and IAA. The treatments with 50,0 ?M 2,4-D; 3,0 ?M TDZ and in the absence of growth regulator didn't present callus formation. After 210 days, it was chosen a callus formed in the culture medium added with 2,5 ?M of TDZ. The selected callus was maintained in the same treatment, however, in liquid medium and under agitation for 60 days at 25 ?C in the darkness, to induce cellular wall and extracellular lignin formation. After this period, the cells suspensions were treated in the following order: MS medium supplemented with sucrose, 2,4-D + kinetin, coumaric acid and without extra supplement. The cells were maintained in these treatments for 30 days. It was used a completely randomized design with four replications. Each plot consisted of an Erlenmeyer with 125 ml of cells suspension culture. For cellular wall lignin extraction and determination, it was used the method of lignin thyoglicolate. The concentration of cellular wall and extracellular lignins were evaluated by UR. For data normality verification it was used the Lilliefors test. The results were statistically analyzed by variance analysis and by Tukey test at 5% level of significance. The highest lignin concentration in the cellular wall (371.4200 ppm) was verified in the treatment EG1pc 0,0584 ?M of sucrose. Extracellular lignins were also analyzed with Wiesner reagent, H NMR and 13C NMR. The highest concentration (134.3167 ppm) of extracellular lignins was verified in the treatment EG3e of 100?M coumaric acid). However, with the results obtained in H NMR and ??CNMR analysis, it was not possible to characterize the polymerization of pure extracellular lignin. A very useful tool for lignification study is the dehydrogenized polymerization (DHP) in vitro. DHPs were produce in cells suspension filtered medium (substrate) at different treatments. For each treatment, it was added H2O2, peroxidase or H2O2 + peroxidase. The DHPs production was done in MS culture medium (without the prior cultivation of cells) as a substrate. It was added to MS medium three different solutions (coniferyl or sinapyl alcohol, peroxidase and the H2O2) by dripping. The products of DHP and the filtered were analyzed by IR and H NMR. The DHPs produced in medium MS without the previous growth of the cells, were analyzed by H NMR and ??CNMR. The IR spectra didn t present any sign in the zone of 1500 cm-1. H NMR also showed no formation of DHPs. Nevertheless, in the, cells suspensions both in H NMR and in ??CNMR analysis presented signs of DHPs, except the DHP1c (MS + 0,0584 ?M sucrose). H NMR presented: 3.86; 6.92; and 4.71 ppm and the ??CNMR: 134,26; 88,21; 65,17; 55,90; and 20,75 ppm. / O objetivo principal desse trabalho foi a elabora??o de modelos para o estudo da lignifica??o. Mudas de Eucalyptus grandis, obtidas a partir da germina??o de sementes in vitro, foram desenvolvidas visando ? obten??o de explantes de segmentos caulinares. Para tal, foram testadas as auxinas TDZ, 2,4-D, ANA e AIA. N?o foi constatada a forma??o de calos nos tratamentos contendo 50,0 ?M de 2,4-D; 3,0 ?M de TDZ e na aus?ncia de regulador de crescimento. Ap?s 210 dias, foi selecionado um calo formado no tratamento contendo 2,5 ?M de TDZ. Este calo foi cultivado no mesmo tratamento, por?m, em meio l?quido e sob agita??o por 60 dias a 25 ?C no escuro, para indu??o da forma??o de lignina da parede celular e ligninas extracelulares. Ap?s este per?odo, as c?lulas em suspens?o foram submetidas aos seguintes tratamentos: meio MS suplementado com sacarose, 2,4-D + cinetina, ?cido p-cum?rico e sem suplemento extra. As c?lulas permaneceram nestes tratamentos por 30 dias. O delineamento experimental utilizado foi o inteiramente casualizado, com 4 repeti??es, sendo a parcela experimental constitu?da por um Erlenmeyer contendo 125 ml de cultura de c?lulas em suspens?o. Para extra??o e determina??o da lignina da parede celular, foi utilizada a t?cnica baseada na lignina tioglicolato. A concentra??o de lignina da parede das c?lulas e das ligninas extracelulares foi avaliada em UV. Para verificar a normalidade dos dados foi usado o teste Lilliefors. Os dados foram submetidos ? an?lise de vari?ncia e as m?dias comparadas pelo teste de Tukey a 5% de probabilidade. A maior concentra??o de lignina na parede celular (371.4200 ppm) foi verificada no tratamento EG1pc 0,0584 ?M de sacarose. Ligninas extracelulares tamb?m foram analisadas com o reagente Wiesner, RMN H e RMN13C. A maior concentra??o (134.3167 ppm) de ligninas extracelulares foi constatada no tratamento EG3e (100?M ?cido p-cum?rico). Por?m, os resultados de RMN H e RMN13C, n?o permitiram caracterizar a polimeriza??o de lignina extracelular pura. No estudo da lignifica??o utilizou-se a t?cnica de polimeriza??o desidrogenativa (DHP) in vitro. DHPs foram elaborados em meios obtidos a partir de filtrados das culturas de c?lulas em suspens?o em diferentes tratamentos. Para cada tratamento, foi adicionado H2O2, peroxidase ou H2O2 + peroxidase. A produ??o de DHPs foi efetuada em meio MS (sem o pr?vio cultivo de c?lulas) como substrato no qual tr?s solu??es (?lcool conifer?lico ou sinap?lico, peroxidase e o H2O2) foram adicionadas por gotejamento. Os produtos das DHPs, juntamente com os filtrados, foram analisados por IV e RMN H. Nos tratamentos realizados em meio MS sem o pr?vio cultivo de c?lulas em suspens?o, os DHPs produzidos foram analisados por RMN H e RMN13C. Os espectros de IV n?o apresentaram sinal na regi?o de 1500 cm-1, bem como as an?lises em RMN H mostraram que n?o houve forma??o de DHPs. Por?m, nas suspens?es celulares tanto em RMN H quanto RMN13C apresentaram sinais de DHP, exceto no tratamento com DHP1c (MS + 0,0584 ?M sacarose). As an?lises em RMN H apresentaram: 3.86; 6.92 e 4.71 ppm e de RMN13C: 134,26; 88,21; 65,17; 55,90 e 20,75 ppm.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-05-26T20:13:29Z No. of bitstreams: 1 2014 - Deyvid Diego Carvalho Maranh?o.pdf: 3511156 bytes, checksum: 353d33c1ec8bd59e0324501bd6a5033a (MD5) / Made available in DSpace on 2017-05-26T20:13:29Z (GMT). No. of bitstreams: 1 2014 - Deyvid Diego Carvalho Maranh?o.pdf: 3511156 bytes, checksum: 353d33c1ec8bd59e0324501bd6a5033a (MD5) Previous issue date: 2014-03-20 / CAPES / Calcareous soils represent a significant portion of agricultural areas of the planet; commonly have high contents of calcium, magnesium, carbonates, associated with high pH values. In general, although there is little information about their attributes, they are located in areas with great potential for agricultural use. This study aimed to evaluate aspects involved in the genesis of calcareous soils of the Bambu? Group in the municipalities of Lavandeira and Aurora, southeast of Tocantins state, using morphological, physical, chemical and mineralogical attributes, and to compare methods for analytical evaluation ofthis soil attributes. Mostly, these soils havea low degree of pedogenetic development, highlighting the pedogenic processes of melanization and calcification, as well as high levels of silt fraction, which are related to the occurrence of siltstones layers associated with the limestone. From the evaluations, it is suggested that the carbonate character or calcic horizon must be included in the large group level in the class of Luvissolos H?plicos in the Brazilian System of Soil Classification. In this study, the profil P4 of toposequence T2 showed high calcium carbonate content, but were classified as "?rtico" respectively; thus omitting a feature of great importance in the management aspect. This attribute should be emphasized in the classification, since it influences the soil pH, available phosphorus content and micronutrients. The use of the suffix "r" should be reviewed in the Manual for Soil Description and Sampling in the Field, as this suffix has been used exclusively to the horizon and / or layer C, omitting this feature to other horizons, as it is featured in profile P1 of toposequence T2. As for the analytical methods, the phosphorus (P) determination more efficiently to calcareous soilsuses a buffered alkaline solution extraction (Olsen et al., 1954), and the use of acidic nature extractors (Mehlich 1) may overestimate the values of available phosphorus. Moreover, the method for determining calcium carbonateequivalent, with the changes proposed by Donagemma et al. (2011), quantified higher concentrations of calcium carbonate (CaCO3), suggesting that other methods have underestimating the CaCO3 content of the samples. As for the particle size analysis of fine earth, the usage of sodium hydroxide as chemical dispersant was more efficient in determining the clay fraction, with better dispersion of this fraction compared to the sodium hexametaphosphate solution. In contrast, the method for organic carbon measurement proposed by Yeomans and Bremner (1988) was more efficient, when compared to the method of Donagemma et al. (2011), as shown by the values similar to the ones obtained from elemental analysis (CHNS-O) in samples pretreated with an acid solution. / Os solos calc?rios representam uma parcela significativa das ?reas agr?colas do planeta; comumente, apresentam elevados teores de c?lcio, magn?sio, carbonatos, associados a elevados valores de pH. De modo geral, embora haja poucas informa??es acerca de seus atributos, est?o em ?reas de grande potencial para a utiliza??o agr?cola. Esse estudo teve como objetivo avaliar aspectos envolvidos na g?nese de solos calc?rios do Grupo Bambu?, nos munic?pios de Lavandeira e Aurora, sudestedo estado do Tocantins, a partir de atributos morfol?gicos, f?sicos, qu?micos e mineral?gicos, bem como comparar m?todos anal?ticos para avalia??o de atributos desses solos. Majoritariamente, esses solos apresentaram baixo grau de desenvolvimento pedogen?tico, destacando-se os processos pedogen?ticos de melaniza??o e calcifica??o, al?m de elevados teores da fra??o silte, o que est? relacionado ? ocorr?ncia de l?minas de siltitos associadas ao calc?rio. A partir das avalia??es realizadas, sugere-se que o car?ter carbon?tico ou horizonte c?lcico devem ser inclu?dos no n?vel de grande grupo na classe dos Luvissolos H?plicos do Sistema Brasileiro de Classifica??o de Solos. Neste estudo, os perfis P1 e P4 da topossequ?ncia T2 apresentaram elevados teores de carbonato de c?lcio, por?m foram classificados como ?L?tico? e ??rtico?, respectivamente, omitindo-se essa caracter?stica que ? de grande relev?ncia no aspecto de manejo. Esse atributo deve ser ressaltado na classifica??o, visto que ? determinante do pH, teor de f?sforo e de micronutrientes dispon?veis. A utiliza??o do sufixo ?r? deve ser revista no Manual de Descri??o e Coleta de Solo no Campo, pois esse sufixo tem sido de uso exclusivo para o horizonte e/ou camada C, omitindo essa caracter?stica para os demais horizontes, assim como caracterizado no perfil P1 da topossequ?ncia T2. Quanto aos m?todos anal?ticos, o m?todo de determina??o de f?sforo (P) mais eficiente para solos calc?rios faz uso de uma solu??o extratora alcalina tamponada (Olsen et al., 1954), sendo que a utiliza??o de extratores de natureza ?cida (Mehlich-1) pode superestimar os teores de P dispon?vel. Por outro lado, o m?todo de determina??o de carbonato de c?lcio equivalente, com altera??es da metodologia proposta pela Donagemma et al. (2011), quantificou maiores teores de carbonatos de c?lcio (CaCO3), sugerindo que os demais m?todos t?m subestimando os teores de CaCO3 das amostras. Quanto ? an?lise granulom?trica da terra fina, o hidr?xido de s?dio, como dispersante qu?mico, mostrou-se mais eficiente na determina??o da fra??o argila do solo, por ter proporcionado maior dispers?o dessa fra??o, quando comparado ? solu??o de hexametafosfato de s?dio. Em contrapartida, o m?todo de determina??o de carbono org?nico proposto por Yeomans e Bremner (1988) mostrou-se mais eficiente em compara??o ao m?todo da Donagemma et al. (2011), apresentando valores mais pr?ximos dos obtido na an?lise elementar (CHNS-O) em amostras pr?-tratadas com solu??o ?cida.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-10-03T17:25:33Z No. of bitstreams: 1 2016 - Denilson Dortzbach.pdf: 2628494 bytes, checksum: 615986b2b9f4992ad6bc0756d35c6669 (MD5) / Made available in DSpace on 2017-10-03T17:25:33Z (GMT). No. of bitstreams: 1 2016 - Denilson Dortzbach.pdf: 2628494 bytes, checksum: 615986b2b9f4992ad6bc0756d35c6669 (MD5) Previous issue date: 2016-06-02 / CAPES / The soils of fine wines producing regions of Santa Catarina State, has particular altitude characteristics resulting from its lithology and high levels of organic matter. That differentiate the soil of the major producing regions of the world wine. The study was conducted in the regions of west and mountain through Santa Catarina, covering over 80 % of the properties where descriptions and collections of 38 modal profiles and vineyards spread over 45 farms were carried out. The objectives were: a) To characterize the distribution and interaction of soil formation factors and chemical weathering rates; b) evaluate and quantify the carbon, humic fractions in the profiles and ?13C in surface horizons of Cambisols; c) To characterize physical and chemically soils of the vineyards; d) To evaluate the agricultural potential through different methods, with subsequent adaptation of the System for Agricultural Assessment of land for the grape culture in addition to formulating a proposal for zoning for the wine industry of the state. The results indicate that pedogenetic soil was strongly influenced by the source material, weather and terrain. Cluster analysis separated by the similarity the soil profiles in three different groups. Predominate classes Cambisols and Nitossols. Humic substances allowed to discriminate the taxonomic orders of soil and showed ?13C in soil with the humic, little change in isotopic signature, and soils with moderate. The observed changes were derived from C3 and C4 plants crops. The vineyards have similar chemical properties, and thus can be grouped in a single region for a determination of Geographical Indication. As for agricultural suitability evaluating different methods indicated that the evaluated profiles have low agricultural aptitude for annual crops. Therefore, it was proposed an adaptation of the evaluation system of agricultural suitability of the land for the growing of vines, taking into account specific conditions of altitude in the SC state. Allied to this, the drafting of the zoning proposal is an important tool for the implementation of new vineyards. / Os solos das regi?es produtoras de vinhos finos de altitude do estado de Santa Catarina apresentam caracter?sticas particulares decorrentes da sua litologia e dos elevados teores de mat?ria org?nica, que os diferenciam dos solos das grandes regi?es produtoras de vinho do mundo. O estudo foi realizado nas regi?es do meio oeste e serrana de Santa Catarina, abrangendo mais de 80 % das propriedades, onde foram realizadas descri??es e coletas de 38 perfis modais e em vinhedos espalhados em 45 propriedades rurais. Os objetivos foram: a) caracterizar a distribui??o e a intera??o dos fatores de forma??o dos solos e a taxa de intemperismo qu?mico; b) avaliar e quantificar os teores de carbono, fra??es h?micas nos perfis e o ?13C em horizontes superficiais de Cambissolos; c) caracterizar f?sica e quimicamente os solos dos vinhedos; e d) avaliar a aptid?o agr?cola atrav?s dos diferentes m?todos, com posterior adapta??o do Sistema de Avalia??o Agr?cola das Terras, para a cultura da videira, al?m de formular proposta de zoneamento para a vitivinicultura do estado. Os resultados indicam que a pedog?nese dos solos foi fortemente influenciada pelos fatores material de origem, clima e relevo. A an?lise do agrupamento separou pela similaridade os perfis de solo em tr?s grupos distintos. Predominam as classes de Cambissolos e Nitossolos. As subst?ncias h?micas permitiram discriminar as ordens taxon?micas de solos e o ?13C revelando pouca altera??o na assinatura isot?pica nos solos com horizonte A h?mico. Nos solos com A moderado foram observadas altera??es decorrentes de mudan?as nas coberturas (plantas C3 ou C4). As ?reas dos vinhedos apresentam atributos qu?micos similares, podendo ser agrupadas em uma regi?o ?nica para eventual determina??o de Indica??o Geogr?fica. Quanto ? aptid?o agr?cola, ? avalia??o atrav?s de m?todos usuais na literatura resultou em classes que indicam baixa aptid?o para culturas anuais. Diante disto, foi proposta adapta??o do sistema de avalia??o da aptid?o agr?cola das terras, para a cultura da videira, considerando condi??es especificas de altitude no estado de SC e associada ? elabora??o de uma proposta de zoneamento para a implanta??o de novos vinhedos.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:44Z No. of bitstreams: 5 lopes.pdf: 4584389 bytes, checksum: 490508b2570ae9d7329524ffc42068ef (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T10:36:20Z (GMT) No. of bitstreams: 5 lopes.pdf: 4584389 bytes, checksum: 490508b2570ae9d7329524ffc42068ef (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T10:36:20Z (GMT). No. of bitstreams: 5 lopes.pdf: 4584389 bytes, checksum: 490508b2570ae9d7329524ffc42068ef (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / A presente disserta??o consiste em dois artigos cient?ficos, sendo um artigo original e outro de revis?o. Em um dos artigos, os autores fizeram uma revis?o da literatura sobre c?ncer e pr?polis, abordando a carcinog?nese experimental induzida quimicamente pelo 9,10-dimetil-1,2-benzantraceno (DMBA), a composi??o qu?mica da pr?polis e seus efeitos quimiopreventivos. O objetivo do artigo de pesquisa foi avaliar a rea??o tecidual da mucosa lingual de hamsters, submetida ? aplica??o di?ria e alternada de DMBA e extrato etan?lico de pr?polis (EEP) de uma apresenta??o comercial. Materiais e m?todos: 60 hamsters foram divididos em tr?s grupos, com dois per?odos experimentais, 13 e 20 semanas. A borda lateral da l?ngua foi submetida ? aplica??o t?pica, di?ria e alternada de DMBA 0,5 % e EEP 30 % (grupo EEP, n = 20), DMBA 0,5 % e extrato aquoso de pr?polis (EAP) (grupo EAP, n = 20), DMBA 0,5 % e soro fisiol?gico (grupo DMBA, n = 20). Realizou-se an?lise da ocorr?ncia dos tipos de altera??es cl?nicas e histol?gicas. Mensurou-se a ?rea e volume das altera??es cl?nicas, a ocorr?ncia das altera??es estruturais (AE) e citol?gicas (AC) do tecido epitelial escamoso com atipias e a ?rea histol?gica dos carcinomas de c?lulas escamosas (CCE). Para a an?lise estat?stica, aplicaram-se os testes ANOVA, qui-quadrado, Kruskal-Wallis e Mann-Whitney. Resultados: Para todas as vari?veis analisadas, n?o houve diferen?as significativas na compara??o entre os grupos nos dois tempos experimentais. Em 13 semanas ocorreu uma ?nica les?o de CCE no grupo EEP. Em 20 semanas, a maior ocorr?ncia de CCE tamb?m foi no grupo EEP. Conclus?o: o EEP, com teor alco?lico de 30 %, favoreceu a rea??o tecidual da inicia??o e da promo??o da carcinog?nese, por mecanismos ainda n?o elucidados. / ABSTRACT This dissertation consists of two papers, the first one is a review and the second is an original paper. In the first article, the authors reviewed the literature on cancer and propolis, addressing experimental carcinogenesis, chemically induced by 9,10-dimethyl-1,2-benzanthracene (DMBA), the chemical composition of propolis and its chemopreventive effects. The aim of the research paper was to evaluate the?tissue response?of tongue mucosa of hamsters, subjected to daily and alternating application of ethanolic extract of propolis (EEP) of a commercial presentation. Materials and methods: 60 hamsters were divided into three groups, and two experimental periods, 13 and 20 weeks. The lateral edge of the tongue underwent daily and alternate topical application of DMBA 0.5% and 30% EEP (EEP group, n = 20), DMBA and 0.5% aqueous extract of propolis (EAP) (EAP group; n = 20), and DMBA 0.5%?saline (DMBA?group, n =?20). The occurrence of types of clinical and histological changes was analysed. The area and volume of clinical changes, the occurrence of structural (AE) and cytological (CA) changes of squamous epithelial tissue with atypia and the histological area of squamous cell carcinoma (SCC) were measured. For statistical analysis, ANOVA, chi-square and Kruskal-Wallis and Mann-Whitney test were applied. Results: For all variables, there were no significant differences when comparing the groups in the two experimental periods. In 13 weeks, a single lesion of SCC in EEP group was observed. At 20 weeks, the highest occurrence of SCC was also in the group EEP. Conclusion: the EEP, with an alcohol content of 30% favored tissue reaction of initiation and promotion of carcinogenesis by mechanisms not yet elucidated.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-09-27T19:46:23Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) artur_amaral_nascimento.pdf: 1903978 bytes, checksum: ba8d36a75a72310e6e7df1f98b9e4705 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-10-09T14:11:03Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) artur_amaral_nascimento.pdf: 1903978 bytes, checksum: ba8d36a75a72310e6e7df1f98b9e4705 (MD5) / Made available in DSpace on 2017-10-09T14:11:03Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) artur_amaral_nascimento.pdf: 1903978 bytes, checksum: ba8d36a75a72310e6e7df1f98b9e4705 (MD5) Previous issue date: 2017 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Objetivou-se com este trabalho avaliar o desempenho agron?mico, por meio da produ??o e valor nutritivo, e realizar an?lise de agrupamento com base nas caracter?sticas morfog?nicas e estruturais de h?bridos de Brachiaria decumbens previamente selecionados pela EMBRAPA Gado de Corte. Foram avaliados 15 h?bridos em delineamento em blocos ao acaso com tr?s repeti??es. Para avalia??o de produ??o e valor nutritivo, foram observadas diferen?as significativas para massa seca total, massa seca da folha, massa seca do colmo, rela??o l?mina:colmo, porcentagem de colmo, porcentagem de material morto, fibra em detergente ?cido, digestibilidade in vitro da mat?ria org?nica e lignina. Para a massa seca total e massa seca de folhas, observou-se maior m?dia para o h?brido R41. Em rela??o ? massa seca de colmo e porcentagem de colmo, o menor valor encontrado foi para o h?brido S16, enquanto o R41 apresentou resultado superior. Consequentemente, o h?brido S16 obteve maior rela??o l?mina:colmo e o R41 a menor. O h?brido S16 apresentou maiores quantidades de fibra em detergente ?cido e lignina. O pior resultado para massa seca total foi registrado para h?brido R147. Tamb?m foram encontradas diferen?as para porcentagem de material morto, no qual o h?brido R128 apresentou maior m?dia. Dentre todos os h?bridos avaliados, o S16 apresentou menor massa seca de colmo, menor porcentagem de colmo e maior rela??o l?mina:colmo, por?m maiores teores de lignina e fibra em detergente ?cido e, consequentemente, menor digestibilidade in vitro da mat?ria org?nica. Aten??o especial deve ser dada ao h?brido R23, que se destacou por apresentar elevada massa de folhas e baixo teor de lignina. Para a an?lise de agrupamento, verificou-se a forma??o de 5 grupos pelas dist?ncias euclidiana quadrada e m?dia e 8 grupos pela dist?ncia de Mahalanobis. Pode-se observar que o grupo 5 reuniu maior quantidade de caracter?sticas com potencial para o melhoramento, em ambas dist?ncias avaliadas. Este grupo apresentou elevados valores para rela??o l?mina:colmo, n?mero de folhas vivas, dura??o de vida das folhas e baixa taxa de senesc?ncia foliar. Entretanto, foi registrado baixo n?mero m?dio de perfilhos. O oposto pode ser observado para o grupo 4. O grupo 1 foi composto pela maioria dos h?bridos, foi poss?vel verificar que os mesmos apresentaram caracter?sticas medianas em rela??o aos demais grupos. As caracter?sticas taxa de alongamento de colmo, n?mero de folhas vivas, dura??o de vida das folhas, n?mero m?dio de perfilhos, taxa de senesc?ncia foliar e rela??o l?mina:colmo foram as que mais contribu?ram para a distin??o dos grupos morfog?nicos. O grupo morfog?nico 5 destacou-se pelo perfil com baixo alongamento de colmos, elevado n?mero de folhas vivas, longevidade e rela??o folha colmo, com baixo n?mero de perfilhos. O grupo 3 foi caracterizado por elevado perfilhamento e rela??o l?mina colmo. Dentre todos os grupos avaliados, o grupo 4 apresentou a maior taxa de senesc?ncia foliar e a menor dura??o de vida das folhas, que permite sugerir que estas plantas podem ser avaliadas em sistemas de pastejo mais intensivo. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / The objective of this study was to evaluate the agronomic response of Brachiaria decumbens hybrids using production and nutritive value traits and to group this plants with the cluster analysis based on morphogenic data. The hybrids were previously selected in the EMBRAPA Beef Cattle Breeding Program. Were evaluated 15 hybrids in complete randomized block design with three replicates. In the agronomic evaluation, significant effect was observed to total, leaves and stems dry mass, leaf:stem ratio, stems and dead material percentage, acid detergent fiber, in vitro organic matter digestibility and lignin. To total and leaves dry mass the higher response was observed to the hybrid R41. To stems dry mass and percentage, the lower value was observed to S16, while R41 had the higher values. In consequence, the S16 plants had a substantially high leaf:stem ratio. The lower production of total dry mass was observed to R147 hybrid. Significant differences also were observed and the R128 plants had the higher value. In the range of hybrid evaluated. Should be emphasized that S16 plants had the lowest stems mass and percentage with the highest leaf:stem ratio, but its results showed high amounts of acid detergent fiber, lignin and low in vitro organic matter digestibility. Special attention also should be given to R23 plants which had a satisfactory leaves mass production with low amounts of lignin. In the clustering analysis, was verified the formation of 5 groups with the square and average Euclidian distance and 8 groups with the Mahalanobis distance. The group 5 (S16) had more brought together more morphogenic characteristics with potential to breeding. This group had elevated values to leaf:stem ratio, number of live leaves, leaf life spam and low senescence rate. Its number of tillers was low, an undesirable characteristic. The opposite was observed to group 4 (R86). The group 1 was composed by the majority of the hybrids and showed medium characteristics in relation to the other groups. The traits stem elongation rate, number of live leaves, leaf life spam, number of tillers, senescence rate and leaf:stem ratio was the characteristics which more contributed to the differentiation of the morphogenic groups. The morphogenic group 5 had an interesting profile with low stems elongation, high number of leaves, longevity of leaves, favorable leaf:stem ratio, but low number of tillers. The group 3 was characterized by elevated number of tillers and leaf: stem ratio. Among the groups obtained, the group 4 showed the highest senescence rate and lowest longevity, which allow suggest these plants need more frequent harvests.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-09-28T19:28:43Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) mariane_rodrigues_ferreira.pdf: 1446137 bytes, checksum: b0c55b008bb6208223dfeaa66f8b1e31 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-10-09T14:21:58Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) mariane_rodrigues_ferreira.pdf: 1446137 bytes, checksum: b0c55b008bb6208223dfeaa66f8b1e31 (MD5) / Made available in DSpace on 2017-10-09T14:21:58Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) mariane_rodrigues_ferreira.pdf: 1446137 bytes, checksum: b0c55b008bb6208223dfeaa66f8b1e31 (MD5) Previous issue date: 2017 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Objetivou-se com este trabalho determinar o melhor m?todo de estima??o dos coeficientes de repetibilidade e as melhores combina??es entre cortes de acordo com a estabiliza??o genot?pica para caracter?sticas agron?micas, estimar par?metros gen?ticos e formar grupos morfofuncionais com base nas caracter?sticas morfog?nicas e estruturais por meio do agrupamento de Otimiza??o de Tocher em gen?tipos de Panicum maximum. Os coeficientes de repetibilidade para produ??o de massa seca total (MST), massa seca foliar (MSF), massa seca do colmo (MSC), porcentagem de folhas (%F), porcentagem de colmo (%C), foram estimados por meio de quatro m?todos: an?lise de vari?ncia (ANOVA), an?lise estrutural com base na m?dia dos coeficientes de correla??o (AECOR), an?lise de componentes principais com base na matriz de covari?ncia (CPCOV) e na matriz de correla??es (CPCOR). Para o estudo da estabiliza??o genot?pica, utilizaram-se os coeficientes estimados pela ANOVA e CPCOR. Para a avalia??o das caracter?sticas morfog?nicas foram estimadas: taxa de aparecimento foliar (TAPF), filocrono (FIL), taxa de alongamento foliar (TALF), taxa de senesc?ncia foliar (TSF), comprimento final da l?mina (CFL), n?mero de folhas vivas (NFV), dura??o de vida das folhas (DVF), taxa de alongamento de pseudocolmo (TALC), n?mero m?dio de perfilhos (NMP), rela??o l?mina:colmo (RLC). Para MST, foram observados coeficientes de repetibilidade variando entre 0,3500 e 0,4300 pelos m?todos da ANOVA e CPCOR, respectivamente. Altos coeficientes de repetibilidade tamb?m foram encontrados para a caracter?stica MSF. Baixos coeficientes de repetibilidade foram observados para %F e %Ce rela??o l?mina:colmo. Para estabiliza??o genot?pica da MST, os melhores coeficientes foram observados para a combina??o entre os cortes 6 a 7 e entre os cortes 5 a 8, enquanto os menores coeficientes foram observados quando se utilizaram apenas os cortes 3 a 4 e de 1 a 2, em ambos os m?todos. Para a rela??o l?mina:colmo, os melhores coeficientes foram registrados para os cortes 6 a 7 pelo m?todo da ANOVA, e 1 a 2 pelo m?todo CPCOR. De maneira geral, a combina??o entre os cortes de 6 a 7, tamb?m proporcionou maior repetibilidade e determina??o, otimizando a estabiliza??o dos gen?tipos para as massas e porcentagens de folha e de colmo. No estudo dos par?metros gen?ticos e agrupamento, foi observado que somente as caracter?sticas TALF, CFL e RLC tiveram o componente vari?ncia gen?tica significativo. Apesar disto, as caracter?sticas TALC, NFV, apresentaram coeficientes de varia??o genot?picos (CVg) superiores aos coeficientes de varia??o residual ou ambiental (CVe). As caracter?sticas TAPF, FIL, DVF e TSF apresentaram valores abaixo da unidade para a raz?o CVg/CVe. Alta raz?o CVg/CVe foi observada para as caracter?sticas RLC, NFV, TALC, TALF, CFL, sendo os maiores coeficientes foram registrados para RLC. Ap?s o agrupamento, constatou-se a forma??o de cinco grupos morfofuncionais. Os grupos que apresentaram maiores valores de TALF foram os grupos 3, 5 e 1 com valores superiores ? m?dia geral de todos os gen?tipos avaliados. Enquanto o grupo 4 obteve menor desempenho para esta caracter?stica. Para a TALC o grupo 2 se destacou, seguido pelo grupo 5. Dentre todos os grupos a maior RLC constada foi para o grupo 4 e para CFL o grupo 3. Conclui-se que os m?todos que proporcionaram os melhores coeficientes de repetibilidade de determina??o foram os dos componentes principais com base na matriz de correla??o e de covari?ncia. Para a estabiliza??o genot?pica, os melhores coeficientes de repetibilidade e determina??o s?o observados para os cortes realizados no segundo per?odo das ?guas. As caracter?sticas TALF, CFL e RLC apresentam variabilidade gen?tica significativa, e as caracter?sticas TAPF, FIL, DVF e TSF apresentam baixa raz?o CVg/CVe e necessitam de maior controle ambiental. Os grupos 3, 5 e 1, apresentam altas taxas de alongamento de folha como mecanismo de ac?mulo de forragem. J? o grupo 4 se destaca pela capacidade de perfilhamento. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / The objective was to evaluate the coefficient of repeatability of the agronomic traits and to estimate the breeding value of morphogenic characteristics to stablish morphofunctional groups in the clustering analysis of Tocher in Panicum maximum genotypes. The repeatability coefficients were estimated by four methods: analysis of variance (ANOVA), structural analysis based on the correlation matrix (EACOR), principal component analysis based on the variance and covariance?s matrix (PCCOV) and principal component analysis based on the correlation matrix (PCCOR). To the genotypic stabilization study, the ANOVA and PCCOR were used. To the morphogenic evaluation, the characteristics were estimated: leaf appearance rate (LAR), phillochron (PHC), leaf elongation rate (LER), leaf senescence rate (LSR), number of live leaves (NLL), leaf life spam (LLS), leaf final length (LFL), stem elongation rate (SER), average number of tillers (ANT) and leaf:stem ratio (LSR). To total dry matter were observed repeatability coefficients ranging from 0.3500 to 0.4300 by the ANOVA and PCCOR methods, respectively. High coefficients of repeatability were estimated to leaf dry mass too. Low coefficients of repeatability were observed to percentage of leaves, stems and leaf:stem ratio. In the genotypic stabilization of total dry mass the higher coefficients were observed between the 6 and 7th harvest and between the 5 and 8th harvest, while the lowest coefficients were observed when the harvests 3 to 4 and 1 to 2 were considered in both methods. To leaf:stem ratio the higher coefficients were observed to harvests between 6 and 7 in the ANOVA method and 1 to 2 in the PCCOR method. In general, the combination between the 6 and 7th harvests also improves the repeatability and determination coefficients, optimizing the stabilization of the genotypes to dry mass and percentage of leaf and stems. In the study of genetic parameters and clustering, were observed significant effect to genetic variance component only to LER, LFL and LSR. In spite of this the characteristic SER and NLL had CVg higher than CVr. The characteristics LAR. PHC, LLS and LSR had CVg/CVr above the unity. High CVg/CVr ratio were observed to LSR, NLL, SER, LER and LFL, so that the highest coefficient observed to LSR. After the clustering analysis was found five morphofunctional groups. The groups with higher LER were 3, 5 and 1 with breeding values above the general mean. The group 4 had lowest LER. The groups 2 and 5 had the highest SER and the highest LSR was observed to the group 4. The group 3 showed high LFL. It was possible to conclude that the total and leaf dry mass have higher coefficient of repeatability, indicating better accuracy in the identification of the superior genotypes of P. maximum. In the genotypic stabilization, the higher coefficient and determination were observed to the harvests realized in the rainy period. The morphogenic characteristics that have the highest genetic variance have more possibility of gains with the selection and the traits with low CVg/CVr ratio need more environmental control. The groups 3, 5 and 1 have high potential to forage production duly its high leaf elongation rate.

Made available in DSpace on 2015-03-13T17:08:21Z (GMT). No. of bitstreams: 1 MariaRFM_ate_cap4.pdf: 3684096 bytes, checksum: 4e584745589bd3c41987d83d9310a07f (MD5) Previous issue date: 1999-03-30 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This dissertation deals with sedimentological and structural framework of the siliciclastic rock of the Serra do Martins Formation (FSM) in the Portalegre, Martins and Santana plateau, located to the south of Potiguar Basin, in the southwest and central Rio Grande do Norte state. This formation, regarded as of Oligo-Miocene age based on intrusive relations of the Miocene Macau volcanics, has a still disputable age due to the lack of appropriate bio and/or chronostratigraphic markers. The FSSM deposits crop out along 650 to 750 m high plateau, as a remanescent sedimentary cover directly overlying topographically uplifted pre-cambrian crystalline rocks. During the last decades, these deposits were interpreted according to a Tertiary paleoclimatic evolutionary model, associated to pedogenetic processes. The sedimentological characterization of the FSM was done through a detailed study of its facies, petrography and diagenetic features. The facies study was based on description of field relations, textures and structures, the piling up of the strata and their lateral variations. The FSM was deposited by an anastomosing to coarse-meandering fluvial system, including deposits of lag, cannel-fill, ouver-bank and flood plain. The petrographic composition of the sediments, coupled to their facies and paleocurrent directions, suggest a rather distal sourcearea, to the south of the present plateau. The diagenetic study identified an incipient grain mechanical compaction, pronounced dissolution of the framework, matrix and/or cement components, intense precipitation of kaolinite, silic and, eventually, iron oxides, besides mechanical infiltration of the clays. Most of these events, regarded in the literature as associated to near-surface conditions (eo or telodiagenesis), indicate the FSM sediments were never deeply buried. Topographic relations along longitudinal and transversal sections reaching the Potiguar Basin to the north identified regional dips that allow to discuss stratigraphic correlations between the FSM and the basin formations. The sedimentological features of the different units and the intrusive relations of the Macau volcanics were also considered in these correlations,which support the Oligo-Miocene age previously accepted for the FSM. Concerning the tectonic framework of the FSM, this work investigated the pre-cambrian to cretaceous heritage and the cenozoic deformation, allowing the recognition of pre-, sin and post-FSM structures. The crystalline basement, belonging to the Serid? Belt, displays NE and WNW foliation trends related to the Brasiliano-age ductile shear zones. In this terrain, brittle-ductile and brittle NE- and NW-trending structures, associated with extensional joints filled with pegmatites and quartz veins, are related to an E-W compression by the end of Brasiliano Cycle. The E-W joints and NE-trending fractures were reactivated by N-S to N-S to NW extension during late Jurassic to Cretaceous times, controlling the emplacement of the Rio Cear?-Mirim basic dyke swarm and the opening of the Potiguar rift basin / Esta disserta??o aborda a caracteriza??o sedimentol?gica e estrutural das rochas silicicl?sticas da Forma??o Serra do Martins (FSM), no contexto dos plat?s de Portalegre, Martins e Santana, situados a sul da Bacia Potiguar, nas por??es sudoeste e central do Rio Grande do Norte. A referida forma??o, admitida por alguns pesquisadores como oligomioc?nica, baseado em rela??es de intrus?o com Vulcanismo Macau, tem idade ainda discut?vel, por n?o apresentar registros bio e/ou crono-estratigr?ficos que a posicionem temporalmente. Os dep?sitos da FSM, nos plat?s estudados, afloram entre as cotas de 650 a 750 m e constituem um capeamento sedimentar remanescente, repousando discordantemente sobre rochas precambrianas do embasamento cristalino, topograficamente elevado. Nas ?ltimas d?cadas, esses dep?sitos foram interpretados por alguns pesquisadores como o resultado de uma evolu??o paleoclim?tica do Terci?rio, associados a processos pedogen?ticos. Neste trabalho, a caracteriza??o sedimentol?gica das rochas da FSM est? representada por descri??es faciol?gicas, identifica??o dos aspectos petrogr?ficos e das fei??es diagen?ticas. A sistem?tica adotada no estudo das f?cies, envolvendo a descri??o dos aspectos de campo (p. ex. texturas e estruturas sedimentares), levantamento de perfis seq?enciais do empilhamento vertical dos estratos e elabora??o de se??es mostrando a suas rela??es laterais, revela que os lit?tipos da FSM s?o oriundos de um sistema fluvial entrela?ado a meandrante grosso. Esse sistema fluvial ? representado principalmente pelos dep?sitos de fundo de canal, preenchimento de canal, transbordamento de canal e de plan?cie de inunda??o. A petrografia, envolvendo os aspectos composicionais, associada a algumas caracter?sticas faciol?gicas e dire??es de paleocorrentes, mostra que a ?rea fonte desse sedimentos esteve relativamente distante, a sul dos plat?s estudados. O estudo diagen?tico caracteriza como eventos principais uma compacta??o mec?nica incipiente dos gr?os, pronunciada dissolu??o dos constituintes do arcabou?o, matriz e/ou cimento, precipita??o intensa de caulinita, s?lica e, eventualmente, ?xidos de ferro, al?m de infiltra??o mec?nica de argilas. Muitos desse eventos, associados na literatura a condi??es superficiais (eo ou telodiag?nese), revelam que os sedimentos da FSM n?o foram submetidos a soterramentos pronunciados. As rela??es topogr?ficas levantadas em se??es aproximadamente longitudinais e transversais ? Bacia Potiguar, permitem identificar algumas condi??es de mergulho atrav?s das quais podem ser discutidos os elos de correla??o entre os dep?sitos da FSM e outras unidades dessa Bacia. Os estudos sedimentol?gicos deste trabalho e as rela??es de intrus?o com o Vulcanismo Macau tamb?m foram utilizadas nessas correla??es, apoiando a idade oligomioc?nica tradicionalmente assumida para a FSM. No tocante ao contexto estrutural da FSM, foram investigadas a heran?a precambriana a cret?cea, e a deforma??o cenoz?ica, incluindo a caracteriza??o de fei??es de deforma??o pr?-, sin- e p?s-FSM. O embasamento cristalino est? estruturado segundo trends NE e ONO associados ?s zonas de cisalhamento d?cteis brasilianas da Faixa Serid?. No substrato cristalino s?o reconhecidas estruturas fr?geis a d?ctil-fr?geis de dire??o NE e NO, associadas a juntas de extens?o preenchidas por pegmatitos e veios de quartzo, oriundas dos esfor?os compressivos E-O dominantes no final do Ciclo Brasiliano. As juntas E-O e fraturas NE foram reativadas por extens?o N-S a NO no final do Jur?ssico ao in?cio do Cret?ceo, sendo preenchidas por diques de diab?sio do Vulcanismo Rio Cear?-Mirim e controlando a abertura do Rifte Potiguar. A deforma??o sin-FSM est? representada por estruturas de fluidiza??o, observadas em algumas exposi??es do plat? de Portalegre, indicando a reativa??o dos cisalhamentos precambrianos. Nas fei??es tect?nica p?s-FSM, foram identificadas duas dire??es principais de lineamentos, NE e NO, al?m de uma outra subordinada, N-S, que em macroescala marcam os trends erosivos dos plat?s. A n?vel de afloramento, essas orienta??es normalmente coincidem com padr?es de fraturamentos os quais, embora possuam poucos indicadores cinem?ticos, mostram em geral uma boa correspond?ncia com a compress?o N-S neo-terci?ria sugerida para a regi?o (Dantas 1998, na Bacia Potiguar). Fraturas NE e NO com cinem?tica condicionada ? compress?o E-O oloc?nica foram tamb?m caracterizadas no dom?nio sedimentar das serras estudadas, controlando a eros?o ou preserva??o da FSM. A ocorr?ncia de marcadores crono-estruturais unusuais (preenchimento de fraturas por travertino, cascalho ou ?xido de ferro) nos levou a investigar a deforma??o holoc?nica no embasamento cristalino dos plat?s, com base nas exposi??es da Grota da Fervedeira, aba norte da Serra de Santana. Os padr?es de fraturamento, reconhecidos nesse riacho, como fei??es de deforma??o neotect?nica, evidenciaram um campo de tens?es mais complexo, atuante desde o Terci?rio superior ao Quatern?rio, onde os esfor?os principais s?o de extens?o em todas a dire??es, esfor?os estes tentativamente atribu?dos a um domo t?rmico associado ao Vulcanismo Macau (de idade mioc?nica), e que parece perdurar at? o Recente

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-01-20T21:18:47Z No. of bitstreams: 1 KeilaMarthaAmorimBarroso_TESE.pdf: 21818921 bytes, checksum: 6e052052d865c5daec54b63d43d919f5 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-01-22T20:15:18Z (GMT) No. of bitstreams: 1 KeilaMarthaAmorimBarroso_TESE.pdf: 21818921 bytes, checksum: 6e052052d865c5daec54b63d43d919f5 (MD5) / Made available in DSpace on 2016-01-22T20:15:18Z (GMT). No. of bitstreams: 1 KeilaMarthaAmorimBarroso_TESE.pdf: 21818921 bytes, checksum: 6e052052d865c5daec54b63d43d919f5 (MD5) Previous issue date: 2014-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / As neoplasias de gl?ndulas salivares exibem uma grande diversidade morfol?gica e comportamentos biol?gicos variados o que suscita o interesse na pesquisa destas les?es. A dissemina??o das c?lulas tumorais ? um passo inicial para a progress?o de neoplasias malignas e, dentro deste contexto, os vasos linf?ticos neoformados s?o considerados essenciais para que ocorra essa dissemina??o. O papel do VEGF (fator de crescimento endotelial vascular) na forma??o dos vasos ? fato conhecido mas, pouco se sabe a respeito de sua participa??o em tumores de gl?ndula salivar. Desta forma, o objetivo deste estudo foi avaliar a express?o do VEGF-C e VEGF-D, a densidade linf?tica tumoral (D2-40) e a prolifera??o endotelial linf?tica (dupla marca??o D2-40/Ki-67) em uma s?rie de neoplasias de gl?ndulas salivares. A amostra foi composta por 20 adenomas pleom?rficos, 20 carcinomas aden?ides c?sticos, 20 carcinomas mucoepiderm?ides e 10 casos de tecido glandular salivar com caracter?sticas de normalidade para efeito comparativo. Todos os casos estudados exibiram express?o positiva para VEGF-C em regi?o peritumoral e intratumoral, n?o sendo encontrada diferen?as de imunoexpress?o entre os grupos. No entanto, o grupo dos carcinomas aden?ides c?sticos demonstrou diferen?a significativa da imunoexpress?o do VEGF-C segundo o padr?o cribriforme e s?lido (p = 0,004). A maioria dos casos constantes do presente estudo, apresentou fraca marca??o para VEGF-D em regi?o peritumoral e intratumoral. Na avalia??o da densidade endotelial linf?tica peritumoral, intratumoral e total, os grupos estudados revelaram um gradiente crescente, com valores menores para o grupo dos adenomas pleom?rficos, seguido dos carcinomas mucoepiderm?ides e carcinomas aden?ides c?sticos. A densidade endotelial linf?tica foi maior entre os tumores malignos do que nos tumores benignos. N?o foi observada correla??o entre a imunoexpress?o de VEGF-C e VEGF-D em rela??o a densidade linf?tica tumoral e a prolifera??o endotelial linf?tica / Salivary gland neoplasms exhibit a great morphological diversity and varied biological behavior which raises the interest in the study of these lesions. The spread of tumor cells is an early step in the progression of malignancies and the neoformed lymphatic vessels are considered essential in tumor dissemination. Vascular endotelial growth fator (VEGF) is a family of proteins involved in angiogenesis e lymphangiogenesis. However, in salivar tumors we have limited information on the expression. The aim of this study was to assess the expression of VEGF-C and VEGF-D, lymphatic vessel density (single-staining D2-40) and lymphatic endothelial proliferation (double labeling D2-40/Ki-67) in a series of salivary glands neoplasms. We selected 20 cases of pleomorphic adenoma, 20 of mucoepidermoide carcinoma, 20 of adenoid cystic carcinoma and 10 tissue sample of normal salivary gland. All cases studied showed positive expression of VEGF-C in intratumoral and peritumoral region, no differences in immunoreactivity was found between the groups. However, the group of adenoid cystic carcinoma showed a significant difference in immunoreactivity of VEGF-C by the cribriform and solid pattern (p = 0.004). Most of the cases included in this study showed weak immunoreactivity for VEGF-D in intratumoral and peritumoral region. In the assessment of lymphatic endotelial density peritumoral, intratumoral and total, the groups showed an increasing gradient, with lower values for the group of pleomorphic adenomas followed by mucoepidermoid carcinoma and adenoid cystic carcinoma. Lymphatic endothelial cell density was higher in malignant than benign tumors. No correlation was observed between the immunoreactivity of VEGF-C and VEGF-D in relation to tumor lymphatic density and lymphatic endothelial proliferation

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-30T19:21:48Z No. of bitstreams: 1 RosineyAraujoMartins_TESE.pdf: 7437829 bytes, checksum: d70e2a5bdc24f23fa0e528ff3972109f (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-30T23:50:23Z (GMT) No. of bitstreams: 1 RosineyAraujoMartins_TESE.pdf: 7437829 bytes, checksum: d70e2a5bdc24f23fa0e528ff3972109f (MD5) / Made available in DSpace on 2016-03-30T23:50:23Z (GMT). No. of bitstreams: 1 RosineyAraujoMartins_TESE.pdf: 7437829 bytes, checksum: d70e2a5bdc24f23fa0e528ff3972109f (MD5) Previous issue date: 2014-07-01 / A distribui??o das altera??es diagen?ticas em reservat?rio silicicl?stico do Cenomaniano Superior da Bacia Potiguar sofreu influ?ncia do arcabou?o estratigr?fico e do sistema deposicional. Se??es s?smicas e perfis geof?sicos de dois po?os perfurados na por??o SW da citada bacia registram superf?cies estratigr?ficas regionais representativas de inunda??es m?ximas relacionadas a evento transgressivo. Na an?lise sequencial de oitenta metros de testemunhos (~450m de profundidade) foram reconhecidas nove f?cies deposicionais cujo padr?o de empilhamento granodecrescente ascendente limita ciclos com base conglomer?tica erosiva (lag) sobreposta por intercala??es de arenitos m?dios a muito finos com estratifica??es cruzadas (acanalada, planar e de baixo ?ngulo) e horizontais (plano-paralela, wave e flaser). O topo dos ciclos ? marcado pela deposi??o de pelitos e desenvolvimento de paleossolos e laguna. A correla??o de f?cies geneticamente relacionadas revela associa??es de preenchimento de canal, crevasse e plan?cie de inunda??o, depositadas em trato de sistema transgressivo. Descri??es detalhadas de setenta e nove l?minas delgadas auxiliadas por an?lises de MEV-EBSD/EDS, DRX e is?topos est?veis em arenitos revelaram composi??o arcosiana e arranjos texturais complexos com abundantes franjas de esmectita cobrindo continuamente constituintes prim?rios, cut?culas mecanicamente infiltradas e poros m?ldicos e intragranulares. Crescimentos epitaxiais de K-feldspato cobrem continua ou descontinuamente gr?os de microcl?nio e ortocl?sio antes de qualquer outra fase. Abundante pseudomatriz da compacta??o de intraclastos lamosos, concentrados ao longo de planos de estratifica??o, localmente substitu?da por calcita macrocristalina e pirita microcristalina e framboidal. Caulinita (livrinhos e vermicular), esmectita microcristalina, minerais de tit?nio microcristalinos e pirita substituem constituintes prim?rios. Porosidade intergranular predomina sobre a m?ldica, intragranular e de contra??o. Os poros s?o mal conectados devido ? presen?a intergranular de esmectita, crescimentos de K-feldspato, argilas infiltradas e pseudomatriz. Os arenitos foram sujeitos a condi??es eodiagen?ticas pr?ximas ? superf?cie e mesodiagen?ticas de soterramento raso. As altera??es diagen?tica reduziram a porosidade e permeabilidade deposicionais principalmente pela precipita??o de franjas de esmectita, compacta??o de intraclastos lamosos ? pseudomatriz e cimenta??o por calcita poiquilot?pica, caracterizando diferentes petrof?cies de reservat?rio. Esses produtos diagen?ticos atuaram como barreiras e desvios ao fluxo de fluido reduzindo qualidade do reservat?rio. / The distribution of diagenetic alterations in Late Cenomanian siliciclastic reservoirs from Potiguar Basin was influenced by the stratigraphic framework and the depositional system. Seismic sections and geophysical logs of two wells drilled in the SW portion of the mentioned basin above register regional stratigraphic surfaces representing maximum floods related to a transgressive event. The sequential analysis of 80 m of drill core (~450 m deep) recognized nine depositional facies with an upwards granodecrescent standard piling that limits cycles with an erosional conglomeratic base (lag) overlain by intercalations of medium to very fine sandstones showing cross bedding (channel, planar and low angled) and horizontal bedding (plane-parallel , wave and flaser). The top of the cycles is marked by the deposition of pelites and the development of paleosoils and lagoons. The correlation of genetically related facies reveals associations of channel fillings, crevasse, and flood plains deposited in a transgressive system. Detailed descriptions of seventy nine thin sections aided by MEV-EBSD/EDS, DRX and stable isotope analyses in sandstones revealed an arcosian composition and complex textural arrays with abundant smectite fringes continuously covering primary components, mechanically infiltrated cuticles and moldic and intragrain pores. K-feldspar epitaxial overgrowth covers microcline and orthoclase grains before any other phase. Abundant pseudomatrix due to the compactation of mud intraclasts concentrate along the stratification planes, locally replaced by macrocristalline calcite and microcrystalline and framboidal pyrite. Kaolinite (booklets and vermicular), microcrystalline smectite, microcrystalline titanium minerals and pyrite replace the primary components. The intergrain porosity prevails over the moldic, intragrain and contraction porosities. The pores are poorly connected due to the presence of intergranular smectite, k-feldspar overgrowth, infiltrated mud and pseudomatrix. The sandstones were subjected to eodiagenetic conditions next to the surface and shallow burial mesodiagenetic conditions. The diagenetic alterations reduced the porosity and the permeability mainly due to the precipitation of smectite fringes, compactation of mud intraclasts onto the pseudomatrix and cementing by poikilotopic calcite characterizing different reservoir petrofacies. These diagenetic products acted as barriers and detours to the flow of fluids thus reducing the quality of the reservoir.

Made available in DSpace on 2014-12-17T15:32:16Z (GMT). No. of bitstreams: 1 DeborahPPI.pdf: 1504501 bytes, checksum: a7d060d1016a25d3b60f572c27bd03bc (MD5) Previous issue date: 2008-10-14 / We investigated the immunohistochemistry expression of claudins -1 and -7 in ameloblastoma and in human dental germs on the pattern of distribution (focal, regional or diffuse), the cells that expressed (if central or peripheral) and the location of that expression in the cell components recital membrane, cytoplasm and nucleus. Among the 29 cases of ameloblastoma, 24 were type solid and 6 unicystic. In 7 mandibular specimens of human fetuses found dental germs from the stage of bud to the crown. We note that the pattern of expression in the dental germs was variable for claudinas studied according to the cell type and stage of differentiation and was invariate only in the cells of stellate reticulum. In epithelium internal of enamel organ, claudin-1 has been decreasing with the progression of differentiation as to claudina-7 that was found in the cells of the peripheral papilla. For ameloblastoma the expression was more significant than that observed in dental germs. Fisher s exact test no found association between the expression of claudinas cells in central and peripheral and the type of ameloblastoma (solid or unicystic). Thus, in general the claudin-1 was positive in the central cell of 93,1% of the cases and in peripheral cells of 51,7%. The claudin-7 was expressed in the cells of all cases central and peripheral cells from 89,7%. For both claudins the distribution was predominantly diffuse cells both in central and peripheral cells. Given our findings it is suggested that the expression of claudins may be indicative of the involvement of these molecules in morphogenetics events culminating with the dental development and that possibly influence the development of neoplastic ameloblastoma / Investigamos a express?o imuno-histoqu?mica das claudinas -1 e -7 em ameloblastomas e germes dentais humanos, avaliando o padr?o de distribui??o (focal, regional ou difuso), as c?lulas que as expressavam (central ou perif?rica) e a localiza??o dessa express?o nos constituintes celulares considerando membrana, citoplasma e n?cleo. Dentre os 29 casos de ameloblastomas, 23 eram do tipo s?lido e 6 unic?sticos. Nos 7 esp?cimes mandibulares de fetos humanos observamos germes dentais desde a fase de capuz at? a fase de coroa. Constatamos que o padr?o de express?o nos germes dentais foi vari?vel para as claudinas estudadas de acordo com o tipo celular e est?gio de diferencia??o assemelhando-se apenas nas c?lulas do ret?culo estrelado. No epit?lio interno a express?o da claudina-1 foi decrescente com a progress?o da diferencia??o enquanto para a claudina-7 foi verificada nas c?lulas perif?ricas da papila. Para os ameloblastomas, de forma geral, a express?o foi mais significativa do que aquela observada nos germes dentais. Foi aplicado o teste estat?stico de Fisher o qual n?o demonstrou associa??o entre a express?o das claudinas nas c?lulas centrais e perif?ricas e o tipo do ameloblastoma (s?lido ou unic?stico). De uma maneira geral, a claudina-1 foi positiva nas c?lulas centrais de 93,1% dos casos e nas c?lulas perif?ricas de 51,7%. A claudina-7 esteve expressa nas c?lulas centrais de todos os casos e nas c?lulas perif?ricas de 89,7%. Para ambas as claudinas a distribui??o foi predominantemente difusa tanto nas c?lulas centrais como nas c?lulas perif?ricas. Diante dos nossos achados sugere-se que a express?o das claudinas pode ser indicativa da participa??o destas mol?culas nos eventos morfogen?ticos que culminam com a forma??o dental e possivelmente influenciam o desenvolvimento neopl?sico dos ameloblastomas