191 |
Comportamento vegetativo e reprodutivo do mamoeiro em diferentes densidades de cultivo org?nico / Vegetative and reproductive behavior of papaya in different densities of organic farming.Rezende, Nilson Costa 18 February 2016 (has links)
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-04-05T11:16:21Z
No. of bitstreams: 1
2016 - Nilson Costa Rezende.pdf: 1161142 bytes, checksum: efc8ca5f40de89a345030c29f9450223 (MD5) / Made available in DSpace on 2017-04-05T11:16:21Z (GMT). No. of bitstreams: 1
2016 - Nilson Costa Rezende.pdf: 1161142 bytes, checksum: efc8ca5f40de89a345030c29f9450223 (MD5)
Previous issue date: 2016-02-18 / The study was conducted at the Integrated State Center for Rural Education - CEIER, in ?guia Branca, the Northwest Region of the Esp?rito Santo. The objective of this study was to evaluate the vegetative and reproductive behavior of papaya plant "Golden Sunrise", for different densities of cultivation under organic production system. The design chosen to effect the implementation of the experiment was randomized blocks, with six treatments (1667, 2167, 2667, 3167, 3667 and 4167 plants.hectare-1) and four replications (blocks). The density plantas.hectare 1667 was adopted as a control, the traditional crops of papaya 'Solo' group. Regarding the vegetative behavior planting density promoted in papaya plant: greater height, following a quadratic regression model; smaller diameter of the trunk, following a quadratic regression model; negatively affected the amount of leaves dropped by papaya plant, following a linear regression model; the presence of active photosynthetic leaves, following a quadratic regression model; the length of the leaves of papaya, following a quadratic regression model and leaf area, following a logarithmic regression model. In terms of floral behavior planting density did not affect the number of fruits and carpelloid pentandric and negatively influenced the number of perfect hermaphrodites fruits, following a quadratic regression model. Finally, for fruit production the density negatively influenced the number and weight of marketable fruit, following a quadratic regression model; positively the productivity of commercial fruits, following a quadratic regression model; the number of discarded fruits (out of commercial standard); negatively length and thickness of the fruit, following a quadratic regression model; negatively diameter of the fruit, following a logarithmic regression model and did not affect the number of seeds per fruit. / O estudo foi conduzido, no Centro Estadual Integrado de Educa??o Rural ? CEIER, situado no munic?pio de ?guia Branca, Regi?o Noroeste do Estado do Esp?rito Santo. O objetivo deste trabalho foi avaliar o comportamento vegetativo e reprodutivo do mamoeiro ?Sunrise Golden?, em rela??o a diferentes densidades de cultivo, sob sistema org?nico de produ??o. O delineamento escolhido para efetuar a implanta??o do experimento foi o de blocos ao acaso, com seis tratamentos (1667, 2167, 2667, 3167, 3667 e 4167 plantas.hectare-1) e quatro repeti??es (blocos). A densidade de 1667 plantas.hectare foi adotada como controle, tradicional nos cultivos de mamoeiro do grupo ?Solo'. Com rela??o ao comportamento vegetativo o adensamento de plantio promoveu nos mamoeiros: maior altura, seguindo um modelo de regress?o quadr?tico; menor di?metro do tronco, seguindo um modelo de regress?o quadr?tico; afetou de forma negativa a quantidade de folhas lan?adas pelos mamoeiros, seguindo um modelo de regress?o linear; a presen?a de folhas fotossinteticamente ativas, seguindo um modelo de regress?o quadr?tico; o comprimento das folhas dos mamoeiros, seguindo um modelo de regress?o quadr?tico e a ?rea foliar, seguindo um modelo de regress?o logar?tmica. Em termos de comportamento floral o adensamento de plantio n?o afetou o n?mero de frutos pent?ndricos e carpeloides e influenciou negativamente o n?mero de frutos hermafroditas perfeitos, seguindo um modelo de regress?o quadr?tico. Finalmente, para produ??o de frutos o adensamento influenciou negativamente o n?mero e o peso dos frutos comercializ?veis, seguindo um modelo de regress?o quadr?tico; positivamente a produtividade dos frutos comercializ?veis, seguindo um modelo de regress?o quadr?tico; o n?mero de frutos descartados (fora do padr?o comercial); negativamente o comprimento e espessura dos frutos, seguindo um modelo de regress?o quadr?tico; negativamente o di?metro dos frutos, seguindo um modelo de regress?o logar?tmico e n?o influenciou o n?mero de sementes por fruto.
|
192 |
As representa??es sociais do bioma cerrado entre os alunos do Curso de Tecnologia em Gest?o Ambiental do Instituto Federal Goiano ? Campus Uruta? / The social representations of the biome Between the students of the Course of Technology in Environmental Management of the Institute Federal Goiano - Campus Uruta?Mendes, Walter da Costa 20 April 2012 (has links)
Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-05-22T12:52:03Z
No. of bitstreams: 1
2012 - Walter da Costa Mendes.pdf: 1106183 bytes, checksum: ab9cc845c11d847e0f282a8723b9fd65 (MD5) / Made available in DSpace on 2017-05-22T12:52:03Z (GMT). No. of bitstreams: 1
2012 - Walter da Costa Mendes.pdf: 1106183 bytes, checksum: ab9cc845c11d847e0f282a8723b9fd65 (MD5)
Previous issue date: 2012-04-20 / The objective of this study was to investigate the social representations of students from the
Tecnologia em Gest?o Ambiental do Instituto Federal Goiano ? Campus Uruta?, on concepts
related to the Cerrado and what is its importance to the environment in general and contrast
the speech of these representations with scientific knowledge, considering that this biome has
become the main alternative for expanding agriculture and cattle raising frontier in Brasil,
because it has a mainly flat with ease to the possibility of mechanization and irrigation water
availability in the region. The research is a qualitative approach, where we designed a semistructured
questionnaire with questions related to the Cerrado and their forms of preservation.
The methodology is based on analysis of the collective subject discourse (DSC) according to
Moscovici's theory of social representations. The target audience were nineteen students from
the sixth grade of the second half of 2010 and twenty-two students from the first grade of the
first half of 2011, of the Course Tecnologia em Gest?o Ambiental of the Instituto Federal
Goiano ? Campus Uruta?, to make a comparison of students when they start the course and
when they finish, watching if the students in the DSC of the sixth period, there have been
advances in scientific knowledge regarding the Cerrado, when compared with the DSC
students of the first period. Participation in the survey was voluntary, and participants had to
submit a term of informed consent, because it is a study in humans, according to the National
Council of Ethics. The results found that the DSCs was both the first and sixth periods are
very similar, and the cerrado objectification appears with the presentation of the State of
Goi?s, suggest agriculture and cattle raising as the best option for investment in productive
arrangements for the cerrado, and still, emphasize the richness of biodiversity in the Cerrado.
But the responses of students in the sixth period had more coherent texts in relation to
scientific knowledge, proving that there was increase in knowledge, some even suggesting
alternatives to mitigate the conflict between agriculture and cattle raising production and
environmental conservation / O objetivo deste trabalho foi investigar as representa??es sociais dos alunos do Curso de
Tecnologia em Gest?o Ambiental do Instituto Federal Goiano ? Campus Uruta?, sobre
conceitos relacionados ao bioma Cerrado e qual sua import?ncia para o meio ambiente em
geral, e contrastar o discurso dessas representa??es sociais com o conhecimento cient?fico,
tendo em vista que esse bioma passou a ser a principal alternativa para a expans?o da fronteira
agropecu?ria no Brasil, pois apresenta um relevo predominantemente plano com facilidade
para mecaniza??o e possibilidade de irriga??o pela disponibilidade h?drica da regi?o. A
pesquisa ? de abordagem qualitativa, onde foi elaborado um question?rio semi-estruturado
com quest?es relacionadas com o bioma Cerrado e suas formas de preserva??o. A
metodologia adotada se baseia em an?lise de discurso do sujeito coletivo (DSC) de acordo
com a teoria moscoviciana de representa??es sociais. O p?blico alvo foram dezenove alunos
do sexto per?odo do segundo semestre do ano de 2010 e vinte e dois alunos do primeiro
per?odo do primeiro semestre do ano de 2011, do Curso de Tecnologia em Gest?o Ambiental
do Instituto Federal Goiano ? Campus Uruta?, para se fazer uma compara??o dos alunos
quando iniciam o curso e quando terminam, observando se no DSC dos alunos do sexto
per?odo, houve avan?o no conhecimento cient?fico em rela??o ao bioma Cerrado, quando
comparado com o DSC dos alunos do primeiro per?odo. A participa??o na pesquisa foi
volunt?ria, sendo que os participantes tiveram que entregar um termo de consentimento livre e
esclarecido, pois se trata de uma pesquisa com humanos, de acordo com o Conselho Nacional
de ?tica em Pesquisa. O resultado encontrado foi que os DSCs tanto do primeiro como do
sexto per?odos s?o bem semelhantes, sendo que o cerrado aparece com objetiva??o na
apresenta??o do Estado de Goi?s, sugerem a agropecu?ria como a melhor op??o de
investimento nos arranjos produtivos para o cerrado, e ainda, d?o ?nfase ? riqueza de
biodiversidade no bioma Cerrado. Por?m as respostas dos alunos do sexto per?odo tiveram
textos mais coerentes em rela??o ao conhecimento cient?fico, comprovando que houve avan?o
no conhecimento, alguns sugerindo inclusive alternativas para amenizar o conflito entre
produ??o agropecu?ria e conserva??o ambiental
|
193 |
A produ????o de pesquisa em educa????o e as pol??ticas p??blicas no DF (2006-2012): uma articula????o necess??riaChaves, Clara de Souza 31 March 2014 (has links)
Submitted by Kelson Anthony de Menezes (kelson@ucb.br) on 2016-11-23T17:01:44Z
No. of bitstreams: 1
ClaradeSouzaChavesDissertacaoparcial2014.pdf: 389135 bytes, checksum: f435020351a8b8ed008941359956c6cb (MD5) / Made available in DSpace on 2016-11-23T17:01:44Z (GMT). No. of bitstreams: 1
ClaradeSouzaChavesDissertacaoparcial2014.pdf: 389135 bytes, checksum: f435020351a8b8ed008941359956c6cb (MD5)
Previous issue date: 2014-03-31 / This work falls within the field of study of the research line of public policies managment
and economy of education from Universidade Cat??lica de Bras??lia (UCB) (Catolic
Bras??lias???s University). The goal of the study was the academic production from the
employees of Education Department of the State of Distrito Federal (SEDF) removed as
researchers to make studies in the scope of the career planning of the mastership of
Distrito Federal, started in 2005 with the Law # 3621 of July 14th of 2005. The study
was focused on Academic production of pos graduated programs strictly speaking
(stricto sensu) in Education developed on seach lines that involves policy, managment
and economy of education from Catolic University of Bras??lia and public policies and
education managment of Brasilia???s University. (UNB). Between 2006 and 2012 turned to
basic education. The methodology adopted on the study was: the qualitative search of
exploratory character, when deal with data source. It was used three qualitative search
methods: Bibliographic search, document analysis, and interview. The dissertation was
splited in 5 chapters. The first charpter deal with education as a inalienable right and
requirement as a practice to citizenship development. The second charpter presentes a
bibliography review about education researching developed on Brasil. The third charpter
show us the portrait of the pos graduated in Brasil and Distrito Federal (Capital). The
fourfh charpter show the public education on Distrito Federal the structure and career
profile of mastership. The charpter five presentes the feedback of documentary search
maded by library system of UNB and UCB on the Educationemployess improvment
school on Distrito Federal and the interviews maked. The results of the search
demonstrate a convergence between the seachers view point, universities and public
institutions when you talk about to stablish a conection between academic production
and the problems and challengers of public education on Distrito Federal. / O presente trabalho insere-se no campo de estudos da linha de pesquisa Pol??ticas
P??blicas, Gest??o e Economia da Educa????o da Universidade Cat??lica de Bras??lia (UCB).
O objeto de estudo foi a produ????o acad??mica dos servidores da (Secretaria de Estado
de Educa????o do Distrito Federal (SEDF) afastados para estudos, no ??mbito do Plano
de Carreira do Magist??rio do Distrito Federal, que entrou em vigor em 2005, com a lei
3.621, de 14 de julho de 2005. O estudo est?? focado na produ????o acad??mica dos
programas de p??s-gradua????o stricto sensu em educa????o, desenvolvida nas linhas de
pesquisa Pol??tica, Gest??o e Economia da Educa????o da Universidade Cat??lica de
Bras??lia (UCB) e Pol??ticas P??blicas e Gest??o da Educa????o da Universidade de Bras??lia
(UnB), entre os anos 2006 e 2012, voltada para a educa????o b??sica. A metodologia
adotada no estudo foi a pesquisa qualitativa de car??ter explorat??rio, no que se refere ??
natureza dos dados foram utilizados tr??s m??todos da pesquisa qualitativa: pesquisa
bibliogr??fica, an??lise documental e entrevistas. A disserta????o est?? dividida em 5
cap??tulos. O cap??tulo primeiro aborda a educa????o como direito inalien??vel e pr??requisito
para a pr??tica da cidadania. O segundo cap??tulo apresenta uma revis??o de
literatura sobre as pesquisas educacionais desenvolvidas no Brasil. O cap??tulo terceiro
apresenta um retrato da p??s-gradua????o stricto sensu no Brasil e no Distrito Federal. O
quarto cap??tulo apresenta a educa????o p??blica no Distrito Federal, sua estrutura e perfil
da carreira magist??rio P??blico. O cap??tulo quinto apresenta os resultados da pesquisa
documental realizada nos sistemas de biblioteca da UCB e UnB, na Escola de
Aperfei??oamento dos Profissionais da Educa????o no DF (EAPE) e das entrevistas
realizadas. Os resultados da pesquisa demonstram uma converg??ncia entre os pontos
de vista de pesquisadores, universidades e institui????es p??blicas no que se refere ??
possibilidade de se estabelecer uma conex??o entre a produ????o acad??mica e o
enfrentamento dos problemas e desafios da educa????o p??blica no Distrito Federal.
|
194 |
Por uma Universidade-Ponte : processos sociais e pr?ticas discursivas na ?rea de psicologia social em programas de p?s-gradua??o do sul do BrasilD'oca, Karen Nunes Montes 14 January 2014 (has links)
Made available in DSpace on 2015-04-14T13:22:19Z (GMT). No. of bitstreams: 1
456495.pdf: 229205 bytes, checksum: 1bfa9d6b264af7d84663185772acc5ea (MD5)
Previous issue date: 2014-01-14 / The objective of this study is to investigate the different practices of research in Social Psychology in Rio Grande do Sul in today s scenario and according to the publication policies in periodicals established by the Improvement Coordination of Higher Education Personnel (known as CAPES). The dissertation comprises two studies: the first seeks to problematize the discourse of neutral and objective scientific development and its effects in the construction of the contemporary Brazilian academic knowledge through the writing of an essay supported by social constructionism. Assuming that scientific development is the result of a social construction and thus is not only a fact but a fact full of value that reflects a worldview, evidencing an ideology and revealing the marks of the historical context in which it inserts itself. Therefore, addressing practices such as: the technicality of scientific language, the corporatization of the academy and of many intellectuals who base their practices according to a capitalist logic; academic productivism and the consequent curriculum management, as well as the phenomenon of academic internationalization. Marked by such practices, the dominant discourse associated with scientific development may have as result the academic practices which consist notions of science and scientific criteria supported by the perspectives of neutrality and objectivity, which implies a process of naturalization. The second part discusses the epistemological diversity and scientific criteria presented in publications in journals (2009-2012) in the area of Social Psychology. To formulate this argument, a desk research was conducted associating the publications of research professors of post-graduate programs established in Rio Grande do Sul with Qualis periodicals classifications formulated by the CAPES Program. Thus, this study seeks to contribute to discussions about the social processes that provide epistemological protagonisms. Therefore, throughout this paper we show how the policies can provide homogeneous Qualis scientific criteria which, especially in Social Psychology, provide greater visibility to epistemologies that favor the construction of patterns and a priori expectations for social behavior. / O objetivo deste trabalho ? investigar as diferentes pr?ticas de pesquisa em Psicologia Social no Rio Grande do Sul, no atual cen?rio da P?s-Gradua??o nacional e segundo as pol?ticas de publica??o em peri?dicos institu?das pela Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES). A disserta??o contempla dois estudos: no primeiro busca-se problematizar o discurso do desenvolvimento cient?fico neutro e objetivo e seus efeitos na constru??o do conhecimento acad?mico brasileiro contempor?neo, atrav?s da escrita de um ensaio e com apoio no construcionismo social. Parte-se do pressuposto de que o desenvolvimento cient?fico ? fruto de uma constru??o social e, logo, n?o ? s? um fato, mas um fato carregado de valor que reflete uma vis?o de mundo, evidencia uma ideologia e revela as marcas do contexto hist?rico no qual se inscreve. Dessa forma, abordamos pr?ticas tais como: o tecnicismo da linguagem cient?fica; a empresarializa??o da academia e de muitos intelectuais que pautam suas pr?ticas segundo uma l?gica capitalista; o produtivismo acad?mico e o conseq?ente gerenciamento de curr?culos; bem como o fen?meno da internacionaliza??o acad?mica. Marcado por essas pr?ticas, o discurso predominante associado ao desenvolvimento cient?fico pode ter como efeito pr?ticas acad?micas que comp?em no??es de ci?ncia e crit?rios de cientificidade amparados nas perspectivas da neutralidade e da objetividade, o que implica em processos de naturaliza??o. O segundo estudo discute a diversidade epistemol?gica e os crit?rios de cientificidade presentes nas publica??es em peri?dicos (2009-2012), na ?rea de Psicologia Social. Para fazer essa discuss?o, realizamos uma pesquisa documental que associa as publica??es de professores-pesquisadores de Programas de P?s-Gradua??o institu?dos no Rio Grande do Sul com as classifica??es Qualis peri?dicos formulada pela CAPES. Desse modo buscamos contribuir com as discuss?es sobre os processos sociais que propiciam os protagonismos epistemol?gicos. Logo, ao longo deste trabalho mostramos como as pol?ticas Qualis podem propiciar crit?rios homog?neos de cientificidade e que, especialmente em Psicologia Social, conferem maior visibilidade a epistemologias que favorecem a constru??o de padr?es e expectativas a priori para o comportamento social.
|
195 |
Variabilidade gen?tica e rea??o a doen?as em acessos de Capsicum baccatum / Genetic variability and disease reaction in Capsicum baccatum accessionsMARTINEZ, Aur?lio Ludovico de Almeida 28 July 2014 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-08-22T19:16:20Z
No. of bitstreams: 1
2014 - Aur?lio Ludovico de Almeida Martinez.pdf: 2604129 bytes, checksum: d6ebe0ec048ae2a4b877b584b5f65e6f (MD5) / Made available in DSpace on 2017-08-22T19:16:20Z (GMT). No. of bitstreams: 1
2014 - Aur?lio Ludovico de Almeida Martinez.pdf: 2604129 bytes, checksum: d6ebe0ec048ae2a4b877b584b5f65e6f (MD5)
Previous issue date: 2014-07-28 / CAPES / Originating from Americas, with Brazil as an important center of diversity, Capsicum occupies an important position among greenery. This study aimed to 1) to assess the genetic similarity and presence of duplicates; 2) to evaluate the resistance to anthracnose disease 3) screening viruses resistance in Capsicum baccatum accessions from Instituto Federal Goiano - Campus Ceres genebank. The genetic similarity was determined by molecular characterization at Embrapa Recursos Gen?ticos e Biotecnologia through RAPD and ISSR markers and morphological descriptors defined for Capsicum spp. According to the molecular and morphological characterization it was confirm the genetic diversity among C. baccatum accessions and the lack of duplicates. The anthracnose resistance was evaluated at 50 and 120 days after the dispersion of pepper powder prepared from infected peppers, considering incidence (presence or absence of lesions in fruits) and disease severity (surface damage percentage to the surface total of fruit) through images processing of diseased fruits. The essay was carried out under field conditions, using augmented randomized complete block design with fifteen plants per plot and three commercial varieties as control. Significant difference was observed especially in disease severity between treatments in both periods observed. The viruses incidence was evaluated in two field experiments in the periods between June-December 2012 and January-June 2013. DAS-ELISA test was performed with absorbance reading at 405 nm on a plate reader, all symptomatic and four asymptomatic plants in all plots was sampled, at 180 days after transplanting in the first experiment, and 160 days after transplanting in second one. The Viruses evaluated were tobamovirus Pepper mild motle virus (PMMoV), the cucumovirus Cucumber mosaic virus (CMV), the tospovirus Groundnut ring spot virus (GRSV), Tomato spot wilt virus (TSWV) and Tomato chlorotic spot virus (TCSV) and potyvirus Potato virus Y (PVY) and Pepper yellow mosaic virus (PepYMV). The percentage of incidence in the first and second period evaluated, were 30% e 7% PMMoV, 6% e 27% CMV, 8% e 12% PVY, 25% e 22% PepYMV, 10% e 2% GRSV, 11% e 0% TCSV, 0% e 11% TSWV. Virus were not detected several accessions studied and are potential sources of virus resistance that should be confirmed on resistance testing under controlled conditions. / Origin?rias das Am?ricas e tendo o Brasil como importante centro de diversidade, as pimentas do g?nero Capsicum ocupam posi??o de destaque entre as hortali?as. O presente trabalho teve como objetivos: 1) verificar a similaridade gen?tica e a presen?a de duplicatas; 2) avaliar a resist?ncia ? Colletotrichum spp.; e 3) avaliar a incid?ncia de viroses em acessos de Capsicum baccatum da cole??o de germoplasma do Instituto Federal Goiano - Campus Ceres. A similaridade gen?tica foi determinada pela caracteriza??o molecular realizada na Embrapa Recursos Gen?ticos e Biotecnologia, por meio de marcadores ISSR e RAPD e descritores morfol?gicos definidos para Capsicum spp. De acordo com a caracteriza??o molecular e morfol?gica p?de-se verificar diversidade gen?tica entre os acessos C. baccatum e a inexist?ncia de duplicatas na cole??o. A resist?ncia ? antracnose foi avaliada aos 50 e 120 dias ap?s a dispers?o de p? de pimenta preparado a partir de frutos infectados, considerando incid?ncia (presen?a e aus?ncia de les?es no fruto) e severidade de doen?a (percentual de superf?cie de les?o em rela??o ? superf?cie total do fruto), a partir do processamento de imagens dos frutos doentes. A avalia??o foi realizada em condi??es de campo, utilizando desenho experimental em blocos completos casualizados aumentados com quinze plantas por parcela e tr?s variedades comerciais como testemunhas. Foi observada diferen?a significativa entre os tratamentos, especialmente na severidade da doen?a, nos dois per?odos observados. A incid?ncia de viroses foi avaliada em dois experimentos conduzidos em campo, nos per?odos entre junho a dezembro 2012 e janeiro a junho de 2013. Foi realizado teste DAS-Elisa, com leitura de absorb?ncia a 405 nm em leitora de placas, em amostras das plantas sintom?ticas e quatro plantas assintom?ticas em todas as parcelas. Os v?rus avaliados foram o tobamovirus Pepper mild motle virus (PMMoV), o cucumovirus Cucumber mosaic virus (CMV), os tospovirus Groundnut ring spot virus (GRSV), Tomato spot wilt virus (TSWV) e Tomato chlorotic spot virus (TCSV) e os potyvirus Potato virus Y (PVY) e Pepper yelow mosaic virus (PepYMV). No per?odo de junho a dezembro de 2012, os v?rus detectados e respectivo percentual de incid?ncia foram PMMoV 30%, CMV 6%, PVY 8%, PepYMV 25%, GRSV 10%, TCSV 11%, TSWV 0%. J? no per?odo de janeiro a junho de 2013, o percentual de incid?ncia dos respectivos v?rus foram: PMMoV 7%, CMV 27%, PVY 12%, PepYMV 22%, GRSV 2%, TCSV 0% e TSWV 11%. Para todos os v?rus estudados, houveram acessos nos quais n?o foi detectada a presen?a do v?rus, com destaque para IFET 64 (n?o detectado PMMoV, CMV, PepYMV e PVY), IFET 273, (n?o detectados PMMoV, PVY, TSWV, TCSV e GRSV, IFET 19 (n?o detectado CMV, PVY, TSWV, TCSV e GRSV).
|
196 |
Desempenho produtivo e composi??o do leite e da carne de caprinos alimentados com res?duo de panifica??o / Performance and composition of milk and goat meat fed with bakery wasteMORENZ, Danilo Antonio 31 May 2016 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-01-26T16:31:14Z
No. of bitstreams: 1
2016 - Danilo Antonio Morenz.pdf: 881703 bytes, checksum: 6cfcd4c0f7c8a5dd52386788991fd786 (MD5) / Made available in DSpace on 2018-01-26T16:31:14Z (GMT). No. of bitstreams: 1
2016 - Danilo Antonio Morenz.pdf: 881703 bytes, checksum: 6cfcd4c0f7c8a5dd52386788991fd786 (MD5)
Previous issue date: 2016-05-31 / CAPES / The aim was evaluate the effect of replacing corn by bakery waste (BW) in goats diet on performance, dry matter and nutrients intake, carcass characteristics, chemical composition and fatty acid profile of the Longissimus dorsi muscle and the food intake, apparent digestibility, nitrogen balance, ruminal parameters, production and composition of milk by goats. Experiment 1: Four levels of inclusion of BW replacing corn (0, 33, 66, 100%) in the diet of kids distributed in a completely randomized design were analyzed. The diets were composed of concentrate and Cynodon spp. hay, with forage: concentrate ratio of 60:40. The intakes of dry matter, organic matter, crude protein, ether extract and carbohydrates decreased linearly, while the non-fiber carbohydrates intake, animal performance, carcass characteristics and chemical composition were not affected by replacement of corn by BW. The elaidic acid content (C18: 1 trans-9) increased in the fatty acid profile of the Longissimus dorsi muscle with the inclusion of RP, which can be used as a substitute for corn meal in goats feed composition. It was concluded that bakery waste can substitute the corn up to 100% in the concentrate mixture without affecting intake, performance and carcass and meat traits of kids. Experiment 2: Bakery waste was added replacing 0; 25; 50; 75 and 100% of the concentrate in dry matter basis. Five Saanen lactating goats, non-pregnant with an average weight of 55.7 kg were arranged in a 5 x 5 Latin Square design. Experimental diets were composed by Cynodon hay and concentrate, in a roughage:concentrate ration of 40:60. The inclusion of BW in diets decreases the intake of ether extract, acid detergent fiber and the coefficients of apparent digestibility of crude protein and ether extract. Different levels of substitution did not affect ruminal pH, but for the concentration of ruminal ammonia was found linear reduction with the level of replacement. The inclusion of BW in diets increased the efficiency of use of N. The bakery waste can replace conventional concentrate in diets for goats. / Objetivou-se avaliar o efeito da substitui??o do milho pelo res?duo de panifica??o (RP) na dieta de cabritos sobre o desempenho, consumo de mat?ria seca e dos nutrientes, caracter?sticas de carca?a, composi??o qu?mica e perfil dos ?cidos graxos do m?sculo Longissimus dorsi e em cabras em lacta??o o consumo alimentar, a digestibilidade aparente, o balan?o de compostos nitrogenados, os par?metros ruminais, a produ??o e a composi??o do leite. Experimento 1: Foram analisados quatro n?veis de inclus?o do RP em substitui??o ao milho (0, 33, 66, 100%) na dieta de cabritos distribu?dos em delineamento inteiramente casualizado. As dietas foram compostas de concentrado e feno de Cynodon spp, com rela??o volumoso:concentrado de 60:40. O consumo de mat?ria seca (MS), mat?ria org?nica (MO), prote?na bruta (PB), extrato et?reo (EE) e carboidratos totais (CT) foi linear decrescente, enquanto que o consumo de carboidratos n?o fibrosos (CNF), o desempenho dos animais, as caracter?sticas de carca?a e a composi??o centesimal n?o foram influenciados pela inclus?o do RP em substitui??o ao milho. O teor de ?cido ela?dico (C 18:1 trans-9) aumentou no perfil de ?cidos graxos do m?sculo Longissimus dorsi com a inclus?o do RP. Conclui-se que o RP pode substituir o milho em at? 100% na mistura do concentrado. Experimento 2: Foram avaliados cinco n?veis de substitui??o do concentrado pelo RP (0, 25, 50, 75, 100%), utilizando-se cinco cabras em lacta??o da ra?a Saanen, com peso m?dio de 55,7 kg, distribu?dos em delineamento em Quadrado Latino 5 X 5. As dietas experimentais foram compostas de concentrado e feno de Cynodon spp, numa rela??o volumoso:concentrado de 40:60 na mat?ria seca. Houve redu??o linear do consumo de EE, do consumo de FDA, da digestibilidade da PB e da digestibilidade do EE. A substitui??o crescente do concentrado pelo RP na dieta n?o influenciou o pH ruminal, por?m reduziu a concentra??o de N-NH3 no l?quido ruminal. A inclus?o do RP em n?veis crescentes nas dietas favoreceu a efici?ncia de utiliza??o de nitrog?nio. O RP pode substituir totalmente o concentrado na dieta de cabras em lacta??o.
|
197 |
A utiliza??o de software de controle de rebanho bovino como ferramenta educativa para o curso de Agropecu?ria / The use of bovine herd control software as educational tool for Agricultural courseCARVALHO, Joethe Moraes de 30 June 2016 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-05-11T18:40:57Z
No. of bitstreams: 1
2016 - Joethe Moraes de Carvalho.pdf: 6116852 bytes, checksum: afde03802608c7b8bd7209ceccfe8dfe (MD5) / Made available in DSpace on 2018-05-11T18:40:57Z (GMT). No. of bitstreams: 1
2016 - Joethe Moraes de Carvalho.pdf: 6116852 bytes, checksum: afde03802608c7b8bd7209ceccfe8dfe (MD5)
Previous issue date: 2016-06-30 / The research aimed to analyze the contributions of the application of a herd management educational software in Animal Production discipline of Technical Course in Agriculture at the Federal Institute of Education, Science and Technology Amazon - IFAM Campus Mau?s. Recent research has motivated this work, as pointed out that one of the major causes of truancy is the lack of practical classes. This is because the search location, to date, has not yet physical infrastructure necessary for implementation of practical activities related to cattle, study focus of Animal Production discipline 3. A computer program has been developed for agricultural education. We sought to evaluate the previous knowledge and opinions of 27 students through a questionnaire and an initial test. educational workshops were held where program was used as an educational tool for explanation of the races themes, food, health, dairy and meat production, reproduction, disposal and cattle weighing. After the workshops, knowledge was again tested by a final evaluation. We sought to evaluate the teaching resource by applying a final questionnaire with open and closed questions. The two discipline of teachers were also questioned. The results showed that the use of software, based pedagogically favored learning efficiently and aroused the interest of students by technology and the interdisciplinary. / A pesquisa objetivou analisar as contribui??es da aplica??o de um software educativo de gerenciamento de rebanhos na disciplina Produ??o Animal do curso T?cnico em Agropecu?ria do Instituto Federal de Educa??o, Ci?ncia e Tecnologia do Amazonas ? IFAM Campus Mau?s. Uma pesquisa recente motivou esse trabalho, pois apontou que uma das maiores causas de evas?o escolar ? a car?ncia de aulas pr?ticas. Isso ocorre porque o local da pesquisa, at? o presente momento, ainda n?o possui infraestrutura f?sica necess?ria ? execu??o de atividades pr?ticas inerentes ? bovinocultura, foco de estudo da disciplina Produ??o Animal 3. Foi desenvolvido um programa de computador para educa??o agr?cola. Procurou-se avaliar os conhecimentos e opini?es pr?vios dos 27 alunos por meio de um question?rio e uma prova inicial. Foram realizadas oficinas pedag?gicas onde o programa foi utilizado como ferramenta educacional para explana??o dos temas de ra?as, alimenta??o, sanidade, produ??o leiteira e de carne, reprodu??o, descarte e pesagem de bovinos. Ap?s as oficinas, o conhecimento foi novamente testado atrav?s de uma avalia??o final. Procurou-se avaliar o recurso did?tico atrav?s da aplica??o de um question?rio final, com perguntas abertas e fechadas. Os dois professores da disciplina tamb?m foram questionados. Os resultados obtidos apontaram que o uso do software, fundamentado pedagogicamente, favoreceu o aprendizado de modo eficiente e despertou o interesse dos alunos pelas tecnologias e pela interdisciplinaridade.
|
198 |
Proposta de abordagem tem?tica com enfoque CTS no ensino de f?sica: Produ??o de energia el?trica / Proposal of thematic approach with CTS focus to physics teaching: Electric energy productionCAMPOS, Lidiane Benites de 03 April 2017 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-06-13T19:12:48Z
No. of bitstreams: 1
2017 - Lidiane Benites de Campos.pdf: 4991873 bytes, checksum: b8396eff5b6aec80b0657e9e4c07eb15 (MD5) / Made available in DSpace on 2018-06-13T19:12:48Z (GMT). No. of bitstreams: 1
2017 - Lidiane Benites de Campos.pdf: 4991873 bytes, checksum: b8396eff5b6aec80b0657e9e4c07eb15 (MD5)
Previous issue date: 2017-04-03 / This work aimed to develop a thematic approach within a CTS (Science-Technology-Society) focus to physics teaching, dealing with the issue of "Electric Energy Production". The CTS focused teaching approaches scientific knowledge in the technological and social context of the learner and relates it to their everyday experiences, leading them to reflect on historical, ethical, political and socioeconomic aspects, and thus providing skills and values that help them to take responsible decisions and attitudes. In our case, the proposal was applied in a public school in the city of Angra dos Reis, in the State of Rio de Janeiro, to be evaluated and reworked according to the needs verified. The theme was chosen to be worked in this place, because the city houses the only nuclear power plant in Brazil, being a subject of extreme importance for the population and that allows the CTS approach with emphasis on the reality of the students. However, the approach was not restricted to the nuclear power plant, but involved all means of producing electric energy, such as hydroelectric, thermoelectric, with its variations of fuels, wave power plant, solar power plants and wind power plants, so that the general theme Production Of Electric Energy was divided into three sub-themes: Hydroelectric Plants, Other Modes of Energy Production and Nuclear Plants. The objective of this approach, besides teaching physical concepts involved in the processes of electric energy production, is also to make students aware of the risks, consequences and benefits of each mode of production, to have them reflect and evaluate the most to be able to actively participate in decision-making processes involving such issues. The final product of this work is a didactic sequence with varied didactic methods and resources, to serve as a guide for other teachers who wish to adopt the proposal. The text presents a brief history of science education in Brazil, its peculiarities and needs that justify the proposal and also presents the CTS movement and its main characteristics, that guide this work. The methodology adopted was the Action-research, which allowed the readjustment of the proposal throughout its application. The results were very promising and showed that the teaching of physics with a CTS approach, practiced through a thematic approach focused on students' reality, can be a means to promote a quality scientific education and to train citizens able to understand the world and the Society in which they live. / Este trabalho teve como objetivo desenvolver uma proposta de abordagem tem?tica com enfoque CTS (Ci?ncia-Tecnologia-Sociedade) para o ensino de f?sica, abordando o tema ?Produ??o de Energia El?trica?. O ensino com enfoque CTS aborda os conhecimentos cient?ficos no contexto tecnol?gico e social do educando e o relaciona com suas experi?ncias cotidianas, levando-o a refletir sobre aspectos hist?ricos, ?ticos, pol?ticos e socioecon?micos, fornecendo assim habilidades e valores que o auxiliem na tomada de decis?es respons?veis e atitudes. No nosso caso, a proposta foi aplicada em uma escola p?blica da cidade de Angra dos Reis, no Rio de Janeiro, para que fosse avaliada e reelaborada de acordo com as necessidades verificadas. O tema foi escolhido para ser trabalhado neste local, pois a cidade abriga a ?nica usina nuclear do Brasil, sendo um assunto de extrema import?ncia para a popula??o e que possibilita o enfoque CTS com ?nfase na realidade dos alunos. Por?m, a abordagem n?o ficou restrita ? usina nuclear, mas envolveu todos os meios de produ??o de energia el?trica, tais como hidrel?tricas, termoel?tricas e suas varia??es de combust?veis, usina de ondas, usinas solares e usinas e?licas, de modo que o tema geral Produ??o de Energia El?trica foi dividido em tr?s subtemas: Usinas Hidrel?tricas, Outros Modos de Produ??o de Energia e Usinas Nucleares. O objetivo desta abordagem, al?m de ensinar conceitos f?sicos que envolvem os processos de produ??o de energia el?trica, ?, tamb?m, conscientizar os alunos dos riscos, consequ?ncias e benef?cios de cada modo de produ??o, lev?-los a refletir e avaliar quais os modos mais vantajosos, para que estejam aptos a opinar e participar ativamente de processos decis?rios envolvendo tais quest?es. O produto final deste trabalho ? uma sequ?ncia did?tica com m?todos e recursos did?ticos variados, para servir como guia para outros professores que desejem adotar a proposta. O texto apresenta um breve hist?rico do ensino de ci?ncias no Brasil, suas peculiaridades e necessidades que justificam a proposta e apresenta tamb?m o movimento CTS e suas principais caracter?sticas, que norteiam este trabalho. A metodologia adotada foi a pesquisa-a??o, o que permitiu a readequa??o da proposta ao longo de sua aplica??o. Os resultados foram muito promissores e mostraram que o ensino de f?sica com enfoque CTS, praticado atrav?s de uma abordagem tem?tica voltada para a realidade dos alunos, pode ser um meio de promover uma educa??o cient?fica de qualidade e formar cidad?os aptos a compreender o mundo e a sociedade em que vivem.
|
199 |
Avalia??o produtiva e econ?mica da suplementa??o a pasto e do confinamento de bovinos de corte com dieta de gr?o inteiro de milho / Productive and economic evaluation of supplementation at pasture and feedlot of beef cattle feeding whole corn dietARA?JO FILHO, Helio Jos? de 22 February 2017 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-08-22T18:12:38Z
No. of bitstreams: 1
2017 - Helio Jos? de Ara?jo Filho.pdf: 1260983 bytes, checksum: 99a5ebc69e0f2db8e73b43a3fbf3d09d (MD5) / Made available in DSpace on 2018-08-22T18:12:38Z (GMT). No. of bitstreams: 1
2017 - Helio Jos? de Ara?jo Filho.pdf: 1260983 bytes, checksum: 99a5ebc69e0f2db8e73b43a3fbf3d09d (MD5)
Previous issue date: 2017-02-22 / FAPERJ / CNPq / The growth of the world population has increased the demand for food, this implies in reduction of the available areas for animal production, which makes imperative the development, evaluation and application of techniques that propitiate the increase of the productivity of Brazilian livestock. Thus, the objective of this study was to evaluate and compare the productive and economic performance of beef cattle finishing under three management and feeding systems, namely: at pasture with mineral supplementation (PSM); at pasture with protein-energy supplementation (SPE); And raised at feedlot with high concentrate diet (AG). For this purpose, 60 castrated male bovines, crossbreed Nelore x Angus, which were distributed in a design Completely randomized, with three systems and twenty animals in each. The study was conducted at Fazenda Tr?s Morros, in the city of Casimiro de Abreu. The means of the studied variables were estimated by LSMEANS and compared by PDIFF, at 5% of probability (p<0.05%), of SAS?. The feedlot (AG) presented shorter finishing time (76 days) and higher weight gain (102.2 kg), total daily average gain (1.35 kg.animal-1.day-1) and average daily gain of carcass (1.02 kg.animal-1.day-1), followed by SPE and PSM, respectively, in relation to the finishing time and the average daily gain of carcass. Regarding weight gain and average daily gain, there was no significant difference (p <0.05) between SPE and PSM. In turn, the average carcass weights (ACW), carcass yields (CY), and carcass gains (CG) of AG (ACW 298.3 kg or 19.9@, CY 55.1% and CG 78.3 kg) and SPE (ACW 288.2 kg or 19.2@, CY 56.1% and CG 68.1 kg) were similar to each other and higher (p<0.05) to PSM. The system PSM had the lowest production costs (effective operational cost R$ 2,277, total operational cost R$ 2,323 and total cost R$ 2,466), while the highest effective operational cost was AG (R$ 2,598) and the highest total operational cost (TOC) and total cost (TC) of SPE (TOC of R$ 2,632 and R$ 2,668). The highest gross margin (R$ 341.1) and net margin (R$ 295.9), net result (R$ 153.0), profitability (5.84%) and simple rentability (4.54%) were obtained by PSM, followed by SPE and AG, in that order. However, when the cash flows were corrected by the general price index - domestic availability (GPI-DA), the gross margins (R$ 199.6) and net margin (R$ 152.2) of PSM remained the highest and followed by SPE and AG (respectively), but the other economic indicators were the lowest among the systems. In this way, the highest net result (R$ 70.7), profitability (2.43%) and simple rentability (1.81%) were observed in SPE. Therefore, the feedlot provided the smallest production cycle and increased animal productivity, the pasture system with mineral supplementation allowed the reduction of production costs, while the concentrate supplementation presented better economic result during the dry season of the year 2015. According to the information obtained, both confinement and supplementation with whole maize diet have the potential to be strategic alternatives to the finishing cattle at exclusively pasture, provided satisfactory market conditions. / O crescimento da popula??o mundial tem elevado a demanda por alimento, isso implica em redu??o das ?reas dispon?veis para produ??o animal, o que torna imperativo o desenvolvimento, avalia??o e aplica??o de t?cnicas que propiciem o aumento da produtividade da pecu?ria brasileira. Desta forma, o objetivo deste estudo foi avaliar e comparar o desempenho produtivo e econ?mico da termina??o de bovinos sob tr?s sistemas de manejo e alimenta??o, a saber: a pasto com suplementa??o mineral (PSM); a pasto com suplementa??o prot?ico-energ?tica (SPE); e confinamento com fornecimento de dieta alto gr?o (AG). Para tanto, foram utilizados 60 bovinos machos castrados, ? sangue Nelore x Angus, distribu?dos em um delineamento inteiramente casualizado, em tr?s sistemas e vinte animais em cada. O estudo foi conduzido na Fazenda Tr?s Morros, no munic?pio de Casimiro de Abreu/RJ. As m?dias das vari?veis estudadas foram estimadas pelo LSMEANS e comparadas pela PDIFF, a 5% de probabilidade (p<0,05), do SAS?. O confinamento (AG) apresentou menor tempo de termina??o (76 dias) e maiores ganho de peso (102,2 kg), ganho m?dio di?rio total (1,35 kg.animal-1.dia-1) e ganho m?dio di?rio de carca?a (1,02 kg.animal-1dia-1), seguido por SPE e PSM, respectivamente, em rela??o ao tempo de termina??o e ao ganho m?dio di?rio de carca?a. No que se refere ao ganho de peso e ao ganho m?dio di?rio total, n?o houve diferen?a significativa (p<0,05) entre SPE e PSM. Por sua vez, os pesos m?dios de carca?a (PMC), os rendimentos de carca?a (RC) e os ganhos de carca?a (GC) de AG (PMC de 298,3 kg ou 19,9 @, RC de 55,1% e GC de 78,3 kg) e SPE (PMC de 288,2 kg ou 19,2 @, RC de 56,1% e GC de 68,1 kg) foram semelhantes entre si e superiores (p<0,05) a PSM. J? o sistema PSM obteve os menores custos de produ??o (custo operacional efetivo de R$ 2.277, custo operacional total de R$ 2.323 e custo total de R$ 2.466), enquanto o maior custo operacional efetivo foi de AG (R$ 2.598) e os maiores custo operacional total (COT) e custo total (CT), de SPE (COT de R$ 2.632 e CT R$ 2.668). As maiores margem bruta (R$ 341,1) e l?quida (R$ 295,9), resultado l?quido (R$ 153,0), lucratividade (5,84%) e rentabilidade simples (4,54%) foram obtidas por PSM, seguidas por SPE e AG, nesta ordem. Por?m, quando os fluxos de caixa foram corrigidos pelo ?ndice geral de pre?os ? disponibilidade interna (IGP-DI), as margens bruta (R$ 199,6) e l?quida (R$ 152,2) de PSM permaneceram as mais elevadas e acompanhadas por SPE e AG (respectivamente), contudo, os demais indicadores econ?micos foram os menores entre os sistemas. Desta forma, os maiores resultado (R$ 70,7), lucratividade (2,43%) e rentabilidade simples (1,81%) foram observados em SPE. Portanto, o confinamento proporcionou o menor ciclo de produ??o e maior produtividade animal, o sistema a pasto com suplementa??o mineral possibilitou a redu??o dos custos de produ??o, enquanto a suplementa??o com concentrado apresentou melhor resultado econ?mico, durante a esta??o seca do ano de 2015. De acordo com as informa??es obtidas, tanto o confinamento quanto a suplementa??o com dieta ? base de milho inteiro possuem potencial para serem alternativas estrat?gicas ? termina??o de bovinos exclusivamente a pasto, desde que presentes condi??es satisfat?rias do mercado.
|
200 |
Utiliza??o de marcadores moleculares na an?lise da caracter?stica de qualidade da carne em caprino (Capra hircus) / Use of molecular markers in the analysis of the meat quality characteristic in goats (Capra hircus)GARCIA, Odair Scatolin Rossafa 26 May 2017 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-09-04T21:36:01Z
No. of bitstreams: 1
2017 - Odair Scatolin Rossafa Garcia.pdf: 1812000 bytes, checksum: 37d6d5a375daf6bc6b0ef40d4687a7f7 (MD5) / Made available in DSpace on 2018-09-04T21:36:01Z (GMT). No. of bitstreams: 1
2017 - Odair Scatolin Rossafa Garcia.pdf: 1812000 bytes, checksum: 37d6d5a375daf6bc6b0ef40d4687a7f7 (MD5)
Previous issue date: 2017-05-26 / Goat meat has lower levels of fat than those found in other types of meat such as beef, pork, sheep and deer, but the lack of selection criteria for slaughter, storage and commercialization of meat leads to a low level because of the lack of standardization of the product presenting unpleasant sensory characteristics. A study of polymorphism, variation in gene expression and the association of these variations with the desired phenotype allows to broaden the understanding of the physiological processes, which helps in the strategies aimed at improving the characteristic of interest, resulting in the expected final phenotype. The objective of the present study was to evaluate polymorphisms and gene expression among some of the most promising genotypes of pleiotropic genes, comparing the polymorphism and expression among groups of animals with greater and lesser weight at slaughter to verify if there is any relation with the weight difference Or softness of the flesh. For this purpose, genotypes of 40 goats from the Saanen and Alpina breeds for the growth hormone (GH) gene, diacylglycerol acyltransferase 1 gene (DGAT1), myostatin gene (MSTN), growth factor gene Similar to insulin 1 (IGF1), fatty acid carrier protein (FATP1) gene, nuclear factor 1 (NF1) gene, gamma peroxisome proliferator activated receptor (PPAR?) gene. After analyzing the association of the different genotypes with the slaughter weight (AP), carcass weight (PC) and meat softness expressed in shear force (FC), some genes were selected for the analysis of expression and association with them Variables. The GH, NF1 and PPAR genes were not evaluated for expression, the first for not having presented a good result for the efficiency analysis of the other two primers due to the lack of substantial data for the preparation of the primers. For the softness test, previously performed in another study by the same team, the longissimus lumborum muscle was used. Polymerase chain reaction (PCR) technique was used for the genotyping and later, for some genes, the digestion of the fragments amplified by restriction enzymes, a technique known as PCR-RFLP. Gene expression was conducted using the Real Time PCR technique (qPCR) and the meat tenderness phenotype was analyzed in a texturometer. The data were statistically related using the SAS GLM procedure. The UNIVARIATE procedure was used to verify the normality of residues of expression of the genes under study (expressed as 2-?Ct) and softness data. The averages were compared by the Tukey test and the Pearson correlations tested by the t test. The polymorphism already described in the GH was also detected in the population studied in the present study, the genotype heterozygous AB presented a mean 2.78kg at slaughter weight more than the AA individuals, for the MSTN the individuals with heterozygous M1M2 genotype presented higher scores for weight at slaughter, while for the IGF1 gene the heterozygous AB animals present less tender meat. The group with lower weight at slaughter showed higher expression of the DGAT1 and FATP genes, which may reflect a higher deposition of fat in the carcass and greater softness, in comparison with the group of higher weight. / A carne caprina apresenta teores de gordura abaixo dos encontrados em outros tipos de carne como a de bovino, su?no, ovino e veado. Entretanto, a falta de crit?rio de sele??o para o abate, estocagem e comercializa??o da carne, acaba por gerar um baixo n?vel de consumo, devido ? falta de padroniza??o do produto apresentando caracter?sticas sensoriais desagrad?veis. Estudo de polimorfismo, varia??o na express?o g?nica e associa??o destas varia??es com o fen?tipo desejado permite ampliar a compreens?o sobre os processos fisiol?gicos, al?m de auxiliar programas de melhoramento gen?tico animal para a sele??o de animais com fen?tipos superiores para a caracter?stica de interesse. O objetivo do presente trabalho foi avaliar a associa??o de polimorfismos e express?o g?nica com a caracter?stica peso ao abate e verificar se h? rela??o entre a diferen?a de peso e a maciez da carne. Para este prop?sito foram identificados inicialmente os gen?tipos de cabritos das ra?as Saanen e Alpina para os seguintes genes: horm?nio do crescimento (GH), diacilglicerol aciltransferase 1 (DGAT1), miostatina (MSTN), fator de crescimento semelhante ? insulina 1 (IGF1), prote?na transportadora de ?cidos graxos (FATP1), fator nuclear 1 (NF1), receptor ativado por proliferadores de peroxissomas gama (PPAR?). Ap?s a an?lise de associa??o dos diferentes gen?tipos com o peso ao abate (PA), peso da carca?a (PC) e maciez da carne expressa em for?a de cisalhamento (FC), foram selecionados alguns genes para a an?lises de express?o e associa??o com as mesmas vari?veis. Os genes GH, NF1 e PPAR n?o foram avaliados quanto a express?o, o primeiro por n?o ter apresentado um bom resultado para as analise de efici?ncia dos primers os outros dois devido ? problemas no genoma refer?ncia para a confec??o dos primers. Para o teste de maciez foi utilizado o m?sculo longissimus lumborum. Para a genotipagem foi utilizada a t?cnica da rea??o em cadeia pela polimerase (PCR) e posteriormente, para alguns genes, digest?o dos fragmentos amplificados por enzimas de restri??o (PCR-RFLP). A express?o g?nica foi conduzida utilizando a t?cnica de PCR em Tempo Real (qPCR) e o fen?tipo de maciez da carne foi analisado em textur?metro. Os dados foram analisados estat?sticamente utilizando o procedimento GLM do SAS. O procedimento UNIVARIATE foi utilizado para verificar a normalidade dos res?duos da express?o dos genes em estudo (expressos com 2-?Ct) e dados de maciez. As m?dias foram comparadas pelo teste de Tukey e as correla??es de Pearson testadas pelo teste de t. O polimorfismo j? descrito no GH foi tamb?m detectado na popula??o estudada no presente trabalho, o gen?tipo heterozigoto AB apresentou m?dia 2,78kg a mais de peso ao abate do que os indiv?duos AA, para a MSTN os indiv?duos com gen?tipo heterozigoto M1M2 apresentaram maiores escores para peso ao abate, enquanto para o gene IGF1 os animais heterozigotos AB apresentam carne menos macia. O grupo com menor peso ao abate apresentou maior express?o dos genes DGAT1 e FATP, o que pode refletir maior deposi??o de gordura de gordura na carca?a e maior maciez, em compara??o com o grupo de maior peso.
|
Page generated in 0.0198 seconds