Les animaux de compagnie présentent un intérêt majeur dans les études cardiovasculaires soit comme modèles expérimentales soit comme modèles spontanées. De plus, le développement de méthodes d'exploration non invasives en médecine vétérinaire est devenu une nécessité à cause de régulations strictes concernant le bien être de l'animal. Un facteur limitant demeure l'exactitude et la fiabilité (répétabilité/reproductibilité) des techniques d'exploration pour leur utilisation répétitive chez des animaux vigiles. La mesure de la pression artérielle systémique (PA) non invasive et l'échocardiographie conventionnelle et avancée ont gagné de la place dans le domaine de recherche ; cependant, il n'existe pas de recommandations spécifiques sur la validation métrologique de méthodes quantitatives utilisées en recherche clinique ou fondamentale en médecine vétérinaire. Le présent travail a premièrement focalisé sur la validation métrologique de la mesure de la PA chez le chien et le chat en étudiant la répétabilité et la reproductibilité de la méthode Doppler (chez les deux espèces) et de la méthode oscillométrique (chez le chien) et en déterminant l'influence du niveau d'expérience de l'opérateur sur la variabilité intra- et inter-jour et le pourcentage de mesures réussies. Puis l'effet de la position de l'animal et du nombre de mesures répétées sur de variables sélectionnées d'échocardiographie bidimensionnelle (2D) et temps-mouvement (TM) a été évalué. De plus, la méthode de l'aire de la surface de l'isovélocité proximale (PISA) pour la quantification de la régurgitation mitrale (RM) ainsi que le strain (St) et strain rate (SR) dérivé du Doppler tissulaire ont été validé chez le chien par le calcul de la répétabilité et la reproductibilité des techniques. Dans un deuxième temps les techniques précédemment validées ont été appliquées chez des chiens sains, des chiens souffrant de maladie valvulaire mitrale dégénérative (MVD) spontanée et dans un modèle expérimental de myopathie centronucléaire (CNM) chez des Labradors retrievers. Toutes les études ont été réalisées à l'Unité de Cardiologie d'Alfort, à l'Ecole Nationale Vétérinaire d'Alfort. Pour les mesures de la PA, toutes les deux études ont montré que l'entrainement est important, notamment pour la mesure de la pression diastolique avec le Doppler, tandis que la répétabilité et reproductibilité sont bonnes pour un opérateur expérimenté. Concernant l'échocardiographie 2D et TM, la technique peut être réalisée chez des chiens couchés ou debout avec une répétabilité/reproductibilité similaire. La fraction régurgitée évaluée par la méthode PISA est une variable répétable et reproductible pour l'évaluation de la RM chez le chien souffrant de MVD. De façon similaire le St et SR peuvent être mesurés de manière fiable chez le chien vigile et leur utilisation combinée avec d'autres paramètres échocardiographiques offre une nouvelle approche à l'étude de la fonction systolique dans cette espèce et peut complémenter l'échocardiographie conventionnelle pour la mesure de la performance myocardique. L'intérêt de ces méthodes validées a été illustré dans des études avec des chiens souffrant de MVD de gravité variable et une différence significative des paramètres étudiés (PA et fraction régurgitée) a été retrouvée dépendant du stade de la maladie. Enfin dans le modèle de CNM, des altérations sous-cliniques ont été détectées et considérées comme significatives étant donnée les faible coefficients de variation de techniques utilisées. En conclusion, le présent travail a évalué la répétabilité et la reproductibilité de quelques techniques d'exploration cardiovasculaires et a démontré l'importance de la validation en les appliquant dans des circonstances cliniques. Cependant ces résultats ne sont valables que dans les conditions spécifiques utilisées dans ces études. / Small animais present a major interest in cardiovascular studies either as experimental or as spontaneous models. Additionally, the development of non-invasive exploration methods in veterinary medicine has been impeding since regulations applying to animais are getting stricter and animal welfare has been the subject of many debates recently. A limitation factor regarding noninvasive cardiovascular exploration by different methods remains the accuracy, and reliability of the different techniques, that is to say, if the methods are repeatable and reproducible, and able to be used in repeated manner in awake animais. Although non-invasive arterial blood pressure (ABP) measurement and conventional, as weil as, advanced echocardiographic techniques have gained place in the domain of veterinary research, there are no specifie guidelines on how to metrologically validate a quantitative imaging technique in veterinary medicine for use in clinical or fundamental research. The present work focused firstly on metrological validation of non-invasive ABP measurement in dogs and cats by assessment of repeatability and reproducibility of Doppler ultrasonography (DU, in both dogs and cats) and high definition oscillometry (in dogs) and by determination of the influence of the observer's level of experience on within-day and between-day variability and the percentage of successful measurements. Then, the effect of animal position and number of repeated measurements on selected 2-dimensional (20) and M-mode echocardiographic variables in heal~hy dogs was evaluated. Moreover, the proximal isoveJocity surface area (PISA) method for quantification of mitral regurgitation (MR) and the tissue Doppler-derived strain (St) and strain rate (SR) technique in the dog were validated by calculation of repeatability and reproducibility. In a second step, the previously validated techniques were applied to healthy dogs, dogs suffering from spontaneous mitral valve disease (MVD) and to an experimental model of centronudear myopathy (CNM) in labrador retrievers. A total of 7 studies were realized. Ali studies were conducted at the Cardiology Unit of Alfort, in the National Veterinary School of Alfort. For ABP measurements, bath studies showed that training is important especially for evaluation of diastolic ABP with DU, whereas repeatability and reproducibility are good for sufficiently experienced observers. Regarding transthoracic 20 and M-mode echocardiography, itcan be performed on dogs in both standing position and lateral recumbency, and the repeatability of measurements is similar. Regurgitant fraction assessed by the PISA method is a repeatable and reproducible variable for the non-invasive evaluation of MR in awake dogs with MVD. This Doppler index is reliable, and its assessment can therefore be recommended for use, in combination with evaluation of other Doppler echocardiographic variables, in longitudinal studies of mitral valve disease in dogs. SimilarJy, myocardial St and SR can be measured non-invasively and in real time from regional myocardial velocities by TOI with good repeatability and reproducibility in the awake dog. The combined use of. St and SR indices with other tissue Doppler parameter's offers a new ultrasound approach to the systolic function in this species and may complement conventional echocardiographic measure of myocardial performance. The interest of those validation methods was firstly iIIustrated in studies involving dogs suffering from MVD of differing severity, where a relevant difference of the evaluated parameters (ABP and regurgitant fraction) was found depending on the stage of the disease.

Cochlear dead regions (DRs) are areas in the cochlea where inner hair cells and/or neurones are functioning so poorly that a sound that causes peak basilar membrane motion in that region is more efficiently detected via off-frequency listening. The Threshold Equalising Noise (TEN) test is a clinical test procedure for detecting DRs. Psychophysical Tuning Curves (PTCs) can be used to identify the boundary frequency of the DR although the clinical importance of doing this has yet to be determined. Some studies have suggested that the reduction of amplification well inside the DR may be beneficial; however, other studies have been unable to replicate these findings in a more typical clinical population. Three studies were completed in order to:1. determine the prevalence of DRs in a clinical sample of the UK adult population,2. investigate repeatability, agreement and clinical feasibility of the TEN-test and fast PTCs in a clinical setting, and 3. determine the benefit of high-frequency amplification in ears with and without DRs, when listening to nonsense syllable speech material in quiet and babble. In the first study, 343 hearing-impaired adults were tested for DRs using the TEN-test. In total, 36% (95% confidence interval 31-41) of these adults had a DR in at least one ear, but frequently at 4 kHz only. Only 3% (1-5) of participants had a DR spanning more than three consecutive frequencies. These findings suggest that DRs usually only span 1 or 2 clinically-relevant frequencies. In the second study, the TEN-test was completed on 70 ears at frequencies between 0.5 and 4 kHz. Fast PTCs were measured on 20 ears at ≥ 2 frequencies. The TEN-test and fast PTCs were highly repeatable on retest (97% and 100%, respectively). There was 87% agreement between the two procedures in terms of the presence of off-frequency listening, with the TEN-test less likely to detect a DR than fast PTCs. Compared to the TEN-test, fast PTCs had a 10% lower ‘conclusive finding’ rate and the test duration was typically 40 minutes longer. Therefore, the TEN-test is more clinically acceptable, but it may underestimate the extent of a DR because of its inability to precisely identify the boundary frequency. In the third study, 18 ears with a high-frequency DR and 18 matched ears without a DR were tested. Vowel-Consonant-Vowel (VCV) stimuli were presented in quiet and babble when listening with an unfiltered and three low-pass filtered hearing aid settings. Best performance was obtained in the unfiltered condition; however the DR group performed significantly poorer than the controls in babble. There was no evidence to support reducing amplification in ears with a DR. However, participants with DRs may benefit from counseling about the limitations of listening in noise. In summary, DRs are relatively prevalent in hearing-impaired adults and can be diagnosed most efficiently in a clinical setting using the TEN-test. However, DRs are often restricted to a narrow frequency range and, in the typical adult clinical population, there is no evidence to support deviating from prescription targets.

This research examined the effects of acclimation to lowered salinity, elevated temperature, and hypoxia on aerobic metabolism of the Gulf killifish, Fundulus grandis, a common estuarine resident of the Gulf of Mexico. Standard metabolic rate (SMR), maximum metabolic rate (MMR), absolute aerobic scope (AAS), and critical oxygen tension (Pcrit) were each influenced by one or more acclimation treatments. Assessing the consistency of these traits measured in the same individuals over time, all were found to be significantly repeatable with no indication that the repeatability of any traits was affected by acclimation conditions. Significant correlations were found between SMR and Pcrit (positively correlated), between SMR and AAS (negatively correlated), between MMR and AAS (positive), and between AAS and Pcrit (negative). This study, therefore, documents the effects of acclimation on these traits, their repeatability, and correlations among them. It further suggests that repeatability of these traits is not context dependent.

The diploma thesis deals with an analysis of the processing machine capability in bus production. The goal is to evaluate the capability of the CNC laser processing machine in conditions of Iveco Czech Republic, Ltd., Vysoke Myto. In the theoretical part, the thesis studies measurements and capability of the processing machine; the practical part describes the present state of production and suggests a solution of the assigned task.

The object of this work is design braking device with high repeatability and reproducibility that is used to analyse the measurement system. I developed this work in cooperation with TRW-DAS Dačice a.s. At the beginning, I made a search of the chassis system, testing of ball joints and steering. Furthermore, I made search of the MSA and the types of braking devices. The rest of the work is focused on the design and the thermal analysis of braking device. Then there are the actual test.

No description available.

Nanopositioners have been developed with electrostatic, piezoelectric, magnetic, thermal, and electrochemical actuators. They move with as many as six degrees of freedom; some are composed of multiple stages that stack together. Both macro-scale and micro-scale nanopositioners have been fabricated. A summary of recent research in micropositioning and nanopositioning is presented to set the background for this work. This research project demonstrates that a dual-stage nanopositioner can be created with microelectromechanical systems technology such that the two stages are integrated on a single silicon chip. A nanopositioner is presented that has two stages, one for coarse motion and one for fine motion; both are fabricated by surface micromachining. The nanopositioner has one translational degree of freedom. Thermal microactuators operate both stages. The first stage includes a bistable mechanism: it travels 52 micrometers between two discrete positions. The second stage is mounted on the first stage and moves continuously through an additional 8 micrometers in the same direction as the first stage. Two approaches to the control of the second stage are evaluated: first, an electrical input is transmitted to an actuator that moves with the first stage; second, a mechanical input is applied to an amplifier mechanism mounted on the first stage after completing the coarse motion. Four devices were designed and fabricated to test these approaches; the one that performed best was selected to fulfill the objective of this work. Thermal analysis of the actuators was performed with previously developed tools. Pseudo-rigid-body models and finite element models were created to analyze the mechanical behavior of the devices. The nanopositioners were surface micromachined in a two-layer polysilicon process. Experiments were performed to characterize the resolution, repeatability, hysteresis, and drift of the second stages of the nanopositioners with open-loop control. Position measurements were obtained from scanning electron micrographs by a numerical procedure, which is described in detail. The selected nanopositioner demonstrated 170-nanometer resolution and repeatability within 37 nanometers. The hysteresis of the second stage was 6% of its full range. The nanopositioner drifted 25 nanometers in the first 60 minutes of operation with a time constant of about 6 minutes. The dual-stage nanopositioner may be useful for applications such as variable optical attenuators or wavelength-specific add--drop devices.

Saab Surveillance in Järfä̈lla constructs complex products, such as radars and electronic support measures. Saab sees an advantage in manufacturing details with additive manufacturing as it enables a high level of complexity. Additive manufacturing is relatively new in the industry and consequently there are uncertainties regarding the process. The purpose of this bachelor thesis was to improve the knowledge of the repeatability of additive manufactured parts as well as compare additive manufactured test rods in two different directions, horizontally and vertically, to subtractive manufactured test rods with a vibration test. The vibration test was conducted to simulate the operative environment where the additive manufactured parts might be implemented in the future. Before the vibration test could be performed, the test rods were designed in a 3D-modeling program and analysed with a finite element method to achieve the required natural frequency range of 100 - 200 Hz and a maximal bending stress of 60 - 80 MPa in the notched area of the test rod. It was concluded that the subtractive manufactured test rods had the highest repeatability. The horizontally additive manufactured test rods had a higher repeatability than the vertically additive manufactured test rods, but the vertically additive manufactured test rods had the highest overall strength. It was also concluded that more studies are needed to ensure that additive manufactured parts can be produced with high repeatability while maintaining the structural integrity. / Saab Surveillance i Järfä̈lla konstruerar komplexa försvarsprodukter som till exempel radarsystem. Additiv tillverkning i metall möjliggör tillverkning av produkter med hög komplexitet, men då tillverkningsprocessen är relativt ny i industrin finns det en stor osäkerhet kring processen. Syftet med detta kandidatexamensarbete var att få en bättre förståelse för repeterbarheten hos additivt tillverkade delar samt att jämföra additivt tillverkade provstavar konstruerade i två olika riktningar, horisontellt och vertikalt, med svarvade provstavar med hjälp av ett vibrationstest. Vibrationstestet genomfördes för att simulera den operativa miljön där de additivt tillverkade detaljerna skulle kunna implementeras i framtiden. Innan vibrationstestet kunde utföras simulerades provstavarnas design i en mjukvara för 3D-modellering. En finit element-analys utfördes även fö̈r att få en egenfrekvens inom intervallet 100 - 200 Hz och en maximal böjspänning mellan 60 - 80 MPa i anvisningen på provstaven. Slutsatsen drogs att de traditionellt bearbetade stavarna hade den högsta repeterbarheten. De horisontellt additivt tillverkade stavarna hade högre repeterbarhet än de vertikalt additivt tillverkade stavarna, men att de vertikalt additivt tillverkade stavarna hade ett längre utmattningsliv. Det kunde även konstateras att fler studier inom ämnet behövs för att kunna säkerställa repeterbarheten hos additivt tillverkade delar utan att behöva kompromissa med hållfastheten.

Clinicians are becoming increasingly aware of the effect of various pathologieso n the characteristicso f saccadice ye movements.A s such, an efficient and non-invasivem eano f measuringe ye-movementisn a clinical environmenti s of interest to many. The aim of this thesis is to investigate the clinical application of a non-invasive eye movement recording technique as a part of a clinical examination. Eye movements were measured using an IRIS 6500 infrared limbal eye tracker, which we customized for the direct recording of oblique eye movements as well as horizontal and vertical. Firstly, the eye-tracker itself was assessed. Visually normal observers made saccadic eye movements to a 10' stimulus in eight directions of gaze. Primary (ANOVA) and secondary analyses (mean error less than 5%) resulted in acceptance that averaging four measurements would give a representative measurement of saccadic latency, peak velocity, amplitude and duration. Test-retest results indicated that this technique gives statistically (± 1.96*STDEVDifference) repeatable responses. Severalf actors that could potentially influence clinically basedm easureso f eye-movementsw ere examined. These included, the effect of ageing, viewing distances, dioptric blur and cataract. The results showed that saccadic latency and durationa re significantly (p< 0.05) longer in older (60-89 years)o bserversc ompared to younger (20-39 years). Peak velocity and amplitude were not significantly affectedb y the age of the observer.A ll saccadicp arameters( SP) were significantly affected by direction (Chapter 5). The compact nature of this eye movement methodology is obtainable since there is no significant effect on viewing distance (300 cm vs. 49 cm) (Chapter 6). There is also no significant effect of dioptric blur (up to +LOODS) on any of the four SP. In contrast, a higher level of defocus (+3.O ODS)h as a larger probability of interfering with the measurementso f peak velocity and duration (Chapter 7). Saccadice ye-movementsw ere also recorded whilst normally sighted subjects wore cataract simulation goggles. The results suggested that the presence of dense cataract introduces significant increases in saccadic latencies and durations. No effect was found on the peak velocities and amplitudes.T he effect of amblyopiao n SP was also investigatedin order to examine if this methodologyi s able to detectn ormal from abnormalr esponses(i . e. increased saccadicla tencies).T his set of data (Chapter9 ) showedt hat using IRIS 6500, longer than normal latencies may be recorded from the amblyopic eye but no consistent effect was found for the other SP (peak velocity, amplitude, duration). overall, the results of this thesis demonstrateth at the IRIS 6500 eye-tracker has many desirable elements (it is non-invasive; comfortable for the observers and gives repeatable and precise results in an acceptable time) that would potentially make it a useful clinical tool as a part of a routine examination.

No description available.