Made available in DSpace on 2015-04-14T13:35:29Z (GMT). No. of bitstreams: 1 438174.pdf: 7206240 bytes, checksum: f165d17261aed0c4f2179085d14a7f93 (MD5) Previous issue date: 2012-03-19 / Considering the high incidence of epilepsy (2-3% of world population) and the high rate of resistance to treatment with available antiepileptic drugs (20-30%) it is essential to be considered and made available new therapeutic options. In epilepsy, like other central nervous system disorders, there is loss of neuronal subpopulations, which represents an important consequence in the pathogenesis of the disease. Therapeutic strategies involving the transplantation of stem cells and gene therapy have been studied in experimental models of epilepsy. The genetic model of EL/Suz epilepsy mouse highlights among the experimental models. In this model, seizures originate close to the parietal lobe and spread to other brain regions such as the hippocampus. Some of the behavioral manifestations that they show are the manipulations of seizures associated with handling and vestibular stimulation, and this behavior is exacerbated by the increase with age of the animal. In order to exploit this potential, our study was to evaluate the effect of administration of bone marrow mononuclear cells in the treatment of genetics epilepsy by peripheral injection of these C57BL/6-EGFP cells in EL/Suz mice. The study was distributed in four groups: I) EL+Control (C), II) EL+Saline (S); III) EL+Bone Marrow Mononuclear Cells Inactivated (BMMCI), IV) EL+Bone Marrow Mononuclear Cells (BMMC). The animals were stimulated from birth with direct observation of behavioral manifestations from 30 days of life. Observation and stimulation of the animals were held once a week with the movement up and down the animal and commuting (goaround) in the four directions. To verify the presence of BMMC transplanted in the hippocampus and other brain structures, other agencies use the technique of polymerase chain reaction (PCR). The observation of the pro-and post-transplant groups was used as a parameter to infer that the transplanted mononuclear cells were able to migrate to the nerve tissue and reduce the number of seizures. Counting the number of seizures was evaluated in the pre and post-transplant record electroencephalographic (EEG) in groups EL+Saline and EL+BMMC for intensity control of seizures. Electrophysiological recordings in brain slices were done to evaluate the synaptic response using the paradigm of paired pulse stimulation. Our results show a gradual reduction in seizure frequency over a period of 240 days reaching 50%. To compare our findings to reduce crises we study the brain electrical activity by recording EEG in which we observed a higher intensity of ictal phase in EL+Saline group compared with the group EL+BMMC. We observed that the animals in the EL+Saline showed a significantly higher neuronal excitability compared to the control group and EL+BMMC proving the therapeutic effect of BMC. In the study of stimulus condition we saw that the three groups were facilitated induction of longterm potentiation (LTP) with a greater amplitude of excitatory postsynaptic potential in the group EL+Saline. Based on data obtained in this study, we conclude that the mononuclear bone marrow may have therapeutic effect on epilepsy genetics in the mouse model of EL/Suz. / Considerando-se a elevada incid?ncia da epilepsia (2-3% da popula??o mundial) e o alto ?ndice de refratariedade ao tratamento com f?rmacos antiepil?pticos dispon?veis (20-30%) torna-se fundamental que sejam estudadas e disponibilizadas novas alternativas terap?uticas. Na epilepsia, a exemplo de outros dist?rbios do sistema nervoso central, h? perda de sub-popula??es neuronais, o que representa uma consequ?ncia importante na patogenia da doen?a. Estrat?gias terap?uticas envolvendo o transplante de c?lulas-tronco e terapia g?nica come?am a ser estudadas em modelos experimentais de epilepsia. Entre os modelos experimentais destaca-se o modelo de epilepsia gen?tica do camundongo EL/Suz. Neste modelo, as crises se originam pr?ximos ao lobo parietal e se propagam para as outras regi?es do c?rebro como o hipocampo. Algumas das manifesta??es comportamentais que os animais apresentam s?o as manipula??es de crises associadas ? estimula??o vestibular, sendo que este comportamento ? exacerbado com o aumento com a idade do animal. Visando explorar este potencial, nosso estudo se prop?s a verificar o efeito da administra??o de c?lulas mononucleares da medula ?ssea no tratamento da epilepsia gen?tica atrav?s da inje??o perif?rica destas c?lulas (C57BL/6-EGFP) em camundongos EL/Suz. O estudo foi distribu?do em 4 grupos: I) EL+Controle (C); II) EL+Salina (S); III) EL+C?lulas-Inativadas (CI); IV) EL+C?lulas Mononucleares (CMMO). Os animais foram estimulados desde o nascimento com observa??o direta das manifesta??es comportamentais a partir de 30 dias de vida. A observa??o e a estimula??o dos animais foram realizadas uma vez por semana com o movimento de subida e descida do animal e movimentos pendulares (ir-vir) nas quatro dire??es. Para verificar a presen?a de CMMO transplantadas no hipocampo bem como em outras estruturas encef?licas e outros ?rg?os utilizamos a t?cnica da polymerase chain reaction (PCR). A observa??o dos grupos pr? e p?s-transplante foi usada como par?metro para que se infira que as c?lulas mononucleares transplantadas foram capazes de migrar para o tecido nervoso e diminuir o n?mero de crises convulsivas. A contagem do n?mero de crises foi avaliada no per?odo pr? e p?s-transplante com registro eletroencefalogr?fico (EEG) nos grupos EL+Salina e EL+CMMO para controle de intensidade das crises. Os registros eletrofisiol?gicos in vitro avaliaram a resposta sin?ptica utilizando o paradigma de estimula??o por pulso pareado em fatias de c?rebro. Nossos resultados mostram uma redu??o gradual da frequ?ncia de crises num per?odo de 240 dias chegando a 50%. Para comparar nossos achados de redu??o de crises fizemos estudo da atividade el?trica cerebral atrav?s do registro EEG onde observamos nos tra?ados eletroencefalogr?ficos uma intensidade maior da fase ictal no grupo EL+Salina comparado com o grupo EL+CMMO. Observamos que os animais do grupo EL+Salina apresentaram uma excitabilidade neuronal significamente maior comparado ao grupo controle e o grupo EL+CMMO comprovando o efeito terap?utico das CMMO. No estudo de est?mulo condicionante podemos observar que os tr?s grupos apresentaram indu??o facilitada de potencia??o de longa dura??o (LTP) com uma maior amplitude do potencial p?ssin?ptico excitat?rio no grupo EL+Salina. Com base nos dados obtidos no presente estudo, conclu?mos que as c?lulas mononucleares da medula ?ssea podem ter efeito terap?utico na epilepsia gen?tica no modelo do camundongo EL/Suz.

Made available in DSpace on 2016-04-28T20:12:56Z (GMT). No. of bitstreams: 1 2006-Andrea Simone Gomes Rente.pdf: 3625015 bytes, checksum: 7b6af838afbe9d1b02c310b4329bba14 (MD5) Previous issue date: 2006-03-10 / This dissertation had as foundation the case study: the creation APA - Alter do Ch?o, located in the area of the Eixo Forte, in the district of Alter do Ch?o, in Santar?m, Par?, Amazonian. The used methodological base was a Bibliographical Revision that it allowed a dialogue of the main themes and, a Research of Field, where we took place open interviews and collection of information in municipal and federal government institutions. The field research had as last end the characterization of the area and the rebuilding of the process of creation of APA - Alter of the Ground through oral report and of participant observation. It was looked for, to leave of those methods, to reach the objectives ends. Such objectives are related with the understanding attempt of as the process of creation of an Area of Environmental Protection - APA, in the case APA - Alter do Ch?o, can be or no seen as inspiration for the maintainable development with freedom in a given area, taking into account aspects political, economical, social and environmental that you/they permeate such process. That process that, in all of the levels, it is sustained starting from the relationship society-nature and starting from the faith that Unit of Conservation - UC of Maintainable Use, as APA's and the development can be configured as possible relationships since there is a perception that the society has freedom and she needs to acquire capacities to do choices, making possible to the members of this to reach a landing in that you/they recognize that his/her survival and of other generations they depend on these choices. In this sense, they are two the main results of this study: the first is related to the perception that APA's while strategies of environmental public politics can be seen as means of making possible the relationship society-nature starting from a democratic participation process in that the main involved actors agree and stimulated her use the same ones in the search of the development. And, according to the positive vision of the development, harnessing it to processes that unchain creation of UC's of Maintainable Use, like APA - Alter do Ch?o. / Esta disserta??o teve como fundamento o estudo de caso: a cria??o APA Alter do Ch?o, situada na regi?o do Eixo Forte, no distrito de Alter do Ch?o, em Santar?m, Par?, Amaz?nia. A base metodol?gica utilizada foi uma Revis?o Bibliogr?fica que permitiu um di?logo das principais tem?ticas referenciadas e, uma Pesquisa de Campo, onde se realizou entrevistas abertas e coleta de informa??es em institui??es governamentais municipais e federais. A pesquisa de campo teve como fim ?ltimo a caracteriza??o da ?rea e a reconstitui??o do processo de cria??o da APA Alter do Ch?o atrav?s de relato oral e de observa??o participante. Buscou-se, a partir desses m?todos, atingir os objetivos fins. Tais objetivos est?o relacionados com a tentativa de compreens?o de como o processo de cria??o de uma ?rea de Prote??o Ambiental - APA, no caso a APA Alter do Ch?o, pode ser ou n?o visto como inspira??o para o desenvolvimento sustent?vel com liberdade em uma dada regi?o, levando em conta aspectos pol?ticos, econ?micos, sociais e ambientais que permeiam tal processo. Esse processo que, em todos os n?veis, se sustenta a partir da rela??o sociedade-natureza e a partir da cren?a de que Unidade de Conserva??o UC de Uso Sustent?vel, como as APA s e o desenvolvimento podem configurar-se como rela??es poss?veis desde que haja uma percep??o que a sociedade tem liberdade e precisa adquirir capacidades de fazer escolhas, possibilitando aos membros desta atingir um patamar em que reconhe?am que a sua sobreviv?ncia e de outras gera??es dependem destas escolhas. Neste sentido, s?o dois os principais resultados deste estudo: o primeiro est? relacionado ? percep??o de que as APA s enquanto estrat?gias de pol?ticas p?blicas ambientais podem ser vistas como meios de viabilizar a rela??o sociedade-natureza a partir de um processo de participa??o democr?tico em que os principais atores envolvidos estejam de acordo e estimulados a usarem as mesmas na busca do desenvolvimento. E, segundo a vis?o positiva do desenvolvimento, atrelando-o a processos que desencadeiam cria??o de UC s de Uso Sustent?vel, como a APA Alter do Ch?o.

Made available in DSpace on 2016-04-28T20:18:33Z (GMT). No. of bitstreams: 1 2009 - Claudia Borges da Costa.pdf: 2611847 bytes, checksum: bac6f485f20793fe627e66f8a53c973e (MD5) Previous issue date: 2009-06-26 / This experiment aimed at recognizing and quantifying the possible macro and microscopic tissue alterations that may occur with the application of bovine pericardium preserved in different concentrations of glutaraldehyde on the abdominal wall. Besides, it also intended to identify the possible interferences in the implant incorporation, according to the variation in the concentration of each solution. Fresh bovine pericardium, after cleaning and the removal of fat, were fractioned and treated with 98% glycerin solution for 30 days (control groups) or with 0.625%, 1% and 1.5% glutaraldehyde solution for 18 days (experimental groups). The fragments were implanted on the abdominal wall of 60 mice (n=15 per treatment) for 7, 14 and 30 days. After each of these periods, five animals of each group were sacrificed for collection, and macro and microscopic analysis of tissue samples. It was observed that the inflammatory reaction in the groups treated with glycerin and 0.625% glutaraldehyde was similar in terms of inflammatory cells infiltration and the repairing process. The groups whose implants were treated with 1% glutaraldehyde chronically presented (30 days) not only more intense fibrosis and necrosis, when compared to other glutaraldehyde groups, but also tissue destruction. These were also observed in the animals that received the pericardium treated with 1.5% glutaraldehyde, analyzed after 14 and 30 days, what leads to the conclusion that this concentration is unviable for the treatment of biological membranes. / Este experimento teve como objetivo reconhecer e quantificar as poss?veis altera??es teciduais macro e microsc?picas que podem ocorrer ap?s a aplica??o de peric?rdio bovino conservado em diferentes concentra??es de glutaralde?do em parede abdominal. Tamb?m foram identificadas poss?veis interfer?ncias na incorpora??o do enxerto de acordo com a varia??o da concentra??o da solu??o de tratamento. Peric?rdios bovinos frescos, ap?s limpeza e retirada de gordura, foram fracionados em fragmentos de 1cm2 e tratados com solu??o de glicerina 98% por 30 dias (grupos controle) ou com solu??o de gutaralde?do 0,625%, 1% e 1,5% por 18 dias (grupos experimentais). Os fragmentos foram implantados na parede abdominal de 60 camundongos (n=15 por tratamento) por 7, 14 e 30 dias e ap?s estes per?odos, cinco animais de cada grupo foram sacrificados para coleta e an?lise macro e microsc?pica das amostras teciduais. Observou-se que a resposta inflamat?ria dos grupos com tratamento com glicerina e com glutaralde?do 0,625% foi semelhante tanto na infiltra??o das c?lulas inflamat?rias quanto no processo de repara??o. Os grupos com implantes tratados com glutaralde?do 1% apresentaram cronicamente (30 dias) a presen?a de fibrose e necrose mais intensa na musculatura da parede abdominal, quando comparado com os outros grupos de glutaralde?do, al?m de destrui??o tecidual. Esta tamb?m foi observada nos animais que receberam peric?rdio tratado com glutaralde?do 1,5% e foram analisados com 14 e 30 dias, o que permitiu a conclus?o de que esta concentra??o ? invi?vel para o tratamento de membranas biol?gicas.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-03T15:45:32Z No. of bitstreams: 1 2010 - Raquel Silva Lisboa.pdf: 4230911 bytes, checksum: e7087ccd29d417f592ca09ec5f49c657 (MD5) / Made available in DSpace on 2016-08-03T15:45:32Z (GMT). No. of bitstreams: 1 2010 - Raquel Silva Lisboa.pdf: 4230911 bytes, checksum: e7087ccd29d417f592ca09ec5f49c657 (MD5) Previous issue date: 2010-04-14 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, CNPq. / Dogs can be infected with various hemoparasites, and the occurrence of co-infections between Ehrlichia canis, Babesia canis, Anaplasma platys, and Hepatozoon canis species is very common, since they have the same tick vector. The objectives of this study were to delineate a multiplex PCR technique for the simultaneous diagnostic of microorganisms of Babesia and Ehrlichia genera in canine blood samples, and to realize the partial characterization of fragments of the 16S rRNA gene of the family Anaplasmataceae agents and, of 18S rRNA gene of Babesia detected in some samples PCR-positive, comparing the sequences obtained with sequences of other strains previously deposited in GenBank. Total DNA of 119 blood samples was extracted, of these, 40 were selected by showing cytoplasmatic inclusions in leukocytes and/or platelets suggesting infection by agents of Anaplasmataceae family (1E to 40E), 37 by showing piroplasms (1B to 37B), and two by presenting structures of both agents (M1 and M2), and finally, 40 samples with negative parasitological diagnostic and hematological exam without alterations. All these samples were tested by PCR to confirm the absence or presence of these hemoparasites, and them utilized in the multiplex PCR delineation. In multiplex PCR reactions the primers A17/EC3 were used to amplify an approximately 600bp region of the 16S rRNA gene of Ehrlichia species and the primers PIRO-A1/PIRO-B were used to amplify an approximately 450bp region of the 18S rRNA gene of Babesia species. Validation of multiplex PCR was performed by real time multiplex PCR. The multiplex PCR was able to simultaneously detect both agents in a DNA sample of a dog naturally co-infected and all the single infections by Babesia, but does not detected all the Ehrlichia infections. The real-time multiplex PCR was more sensitive in detect both single and also co-infections, as well as positive DNA mixtures for the two agents. The sequencing results confirmed the isolates identity, and that the primers PIRO-A1/PIRO-B also amplified the DNA of Hepatozoon canis. Phylogenetic analysis indicated that E. canis, A. platys, B. canis and H. canis species found in this study showed close similarities with sequences previously deposited in GenBank, forming monophyletic groups. / Os c?es podem se infectar com diversos hemoparasitos, sendo muito comum a ocorr?ncia de coinfec??es entre as esp?cies Ehrlichia canis, Babesia canis, Anaplasma platys e Hepatozoon canis, visto que possuem o mesmo carrapato vetor. Este estudo teve como objetivos delinear uma t?cnica de PCR multiplex para diagnosticar simultaneamente microrganismos dos g?neros Ehrlichia e Babesia em amostras de sangue de c?es e realizar a caracteriza??o parcial de fragmentos do gene 16S rRNA de agentes da fam?lia Anaplasmataceae e do gene 18S rRNA de Babesia detectados em algumas amostras positivas pela PCR, comparando as sequ?ncias obtidas com as sequ?ncias de outras cepas depositadas previamente no GenBank. O DNA total de 119 amostras de sangue foi extra?do. Destas, 40 foram selecionadas por apresentar inclus?es citoplasm?ticas em leuc?citos e/ou plaquetas sugestivas de infec??o por agentes da fam?lia Anaplasmataceae (1E a 40E), 37 por apresentar formas parasit?rias de piroplasm?deos (1B a 37B), duas por apresentar estruturas de ambos os agentes (M1 e M2) e, finalmente, 40 amostras com diagn?stico parasitol?gico negativo e exame hematol?gico sem altera??es. Todas estas amostras foram testadas por PCR, para a confirma??o da aus?ncia ou presen?a destes hemoparasitos, e depois utilizadas no delineamento da PCR multiplex. Nas rea??es de PCR multiplex utilizou-se os oligonucleot?deos iniciadores A17/EC3 que amplificam um produto de aproximadamente 600pb de uma por??o do gene 16S rRNA de esp?cies de Ehrlichia e os oligonucleot?deos iniciadores PIRO-A1/PIRO-B que amplificam um produto de aproximadamente 450pb de uma por??o do gene 18Sr RNA de esp?cies de Babesia. A valida??o da PCR multiplex foi realizada por PCR multiplex em tempo-real. A PCR multiplex foi capaz de detectar simultaneamente os dois agentes em uma amostra de DNA de um c?o naturalmente coinfectado e todas as infec??es individuais por Babesia, mas n?o detectou todas as infec??es por Ehrlichia. A PCR multiplex em tempo real foi mais sens?vel em detectar tanto infec??es ?nicas quanto coinfec??es, al?m de misturas de DNA positivo para os dois agentes. Os resultados dos sequenciamentos confirmaram a identidade dos isolados, e que os oligonucleot?deos PIRO-A1/PIRO-B amplificaram tamb?m, o DNA de Hepatozoon canis. As an?lises filogen?ticas indicaram que as esp?cies de E. canis, A. platys, B. canis e H. canis encontradas neste estudo possuem similaridades pr?ximas com sequ?ncias previamente depositadas no GenBank, formando grupos monofil?ticos.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-24T13:37:53Z No. of bitstreams: 1 2011- Veronica da Silva Cardoso.pdf: 2067813 bytes, checksum: a52c401412243d162e7030dda12cdf48 (MD5) / Made available in DSpace on 2016-08-24T13:37:53Z (GMT). No. of bitstreams: 1 2011- Veronica da Silva Cardoso.pdf: 2067813 bytes, checksum: a52c401412243d162e7030dda12cdf48 (MD5) Previous issue date: 2011-04-28 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Piperine interference (amide extracted from black pepper) added to the diet of broiler chickens experimentally intoxicated by aflatoxin B1 (mycotoxin of great importance in the poultry sector) and it?s chemoprotective capacity were the main goal of this work. The experiment was divided into two assays: (i) The first assay was carried out to determine the effects of different concentrations of piperine (0, 60, 120, 180 ppm) and it?s possible toxicity in broiler chickens diets. Ninety six male chicks (Cobb), seven days old were used, being randomly allocated into four experimental groups (n=24) during 35 consecutive days. The following parameters were evaluated: biochemical, hematological, histopathological (proventriculus, gizzard, liver, kidney), histomorphometric (small intestine) and zootecnic. The concentration of 60 ppm of piperine in the diet was safe for broilers, showing better performance of broilers on period from 36 to 42 days old. The concentration of 180 ppm caused leukopenia and concentrations of 120 and 180 ppm was observed decrease in the number of heterophils and monocytes. Hepatotoxicity was observed by elevated AST enzyme activity, histopathological changes and decreased absorption surface in the segments (jejunum and ileum) of small intestine were observed for both 120 and 180 ppm concentrations. (ii) In the second assay, 60 broilers with nine days old divided into four groups: control, piperine (60 ppm added to diet), aflatoxin B1 (0.5 mg of aflatoxin B1.Kg-1 of body weight, orally) and piperine associated aflatoxin B1, were evaluated by effect chemoprotector of piperine against toxics effects of aflatoxin B1 being evaluated for zootecnic, biochemical, histopathological and histomorphometric parameters, toxic heterophils in peripheral blood and genotoxic by comet assay and micronucleus were also determined. No changes in the performance parameters were observed after this experiment. Broiler chickens intoxicated with AFB1 (0.5 mg of aflatoxin B1.kg-1 of body weight ) showed: decreased body weight gain and increased feed conversion; reduced carcass and cuts yields; liver toxicity, with increased relative weight of the liver and heart, macroscopic variations of hepatic parenchyma and increase of liver enzymes activity; kidney enzymes increase without evidence of renal tissue damage macroscopic or microscopic; leukopenia with significant reduction of lymphocytes and heterophils; reduction in absorptive surface due to the reduction of the length and width of the villi of all studied segments of small intestine; presence of toxic heterophils. The cytotoxic and genotoxic effects of aflatoxin B1 described above were significantly reduced or absent in the group of broiler intoxicated with aflatoxin B1 and fed with with piperine. No significant difference between piperine associated aflatoxin B1 in control and piperine groups were observed. The addition of 60 ppm of piperine in the diet of broiler chickens was safe, promoting beneficial effect both in zootecnic parameters and in poultry health, preventing toxic effects of aflatoxin B1in broiler chickens. / A interfer?ncia da piperina (amida extra?da da pimenta do reino) adicionada ? ra??o de frangos de corte intoxicados experimentalmente por aflatoxina B1 (micotoxina de grande relev?ncia no setor av?cola) e sua capacidade quimioprotetora foram o principal objetivo deste trabalho. O experimento foi dividido em dois ensaios: (i) O primeiro ensaio foi realizado para determinar os efeitos de diferentes concentra??es de piperina (0, 60, 120 e 180 ppm) foram avaliados e sua poss?vel toxicidade. Noventa e seis pintos (Cobb), com 7 dias de idade foram divididos aleatoriamente em 4 grupos (n=24), por 35 dias consecutivos. Os par?metros avaliados foram: hematol?gicos, bioqu?micos, histopatol?gicos (proventr?culo, moela, f?gado e rim), histomorfom?trico (intestino delgado) e par?metros zoot?cnicos. A concentra??o de 60 ppm de piperina adicionada ? ra??o foi segura para frangos de corte, tendo ainda resultado em melhor desempenho dos frangos na fase final (36-42 dias de idade). A concentra??o de 180 ppm promoveu leucopenia e nas concentra??es de 120 e 180 ppm foi observada diminui??o do n?mero de heter?filos e mon?citos; hepatotoxicidade, com eleva??o da enzima AST e altera??es histopatol?gicas em ambas as concentra??es; diminui??o da superf?cie de absor??o nos segmentos (jejuno e ?leo) do intestino delgado, por?m, sem altera??o dos par?metros zoot?cnicos. (ii) Para o segundo ensaio com a concentra??o de 60ppm de piperina: 60 frangos com 9 dias de idade, foram divididos em 4 grupos (n=15): grupo controle, grupo aflatoxina B1 (0,5 mg aflatoxina B1.kg-1 de peso vivo por via oral), grupo piperina (60 ppm adicionada ? ra??o) e grupo piperina associada a aflatoxina B1, determinando-se a capacidade quimioprotetora da piperina sendo avaliados os par?metros zoot?cnicos, hematol?gicos, bioqu?micos, histopatol?gicos, histomorfom?tricos, os efeitos genot?xicos da aflatoxina B1 pelo teste do cometa e do micron?cleo, presen?a de heter?filos t?xicos no sangue perif?rico. Os frangos intoxicados com aflatoxina B1 (0,5 mg de aflatoxina B1.Kg-1 de peso vivo) apresentaram: diminui??o do ganho m?dio de peso e piora da convers?o alimentar; diminui??o do rendimento de carca?a e cortes; hepatotoxicidade, com aumento de peso relativo do f?gado e cora??o, varia??es macrosc?picas do par?nquima hep?tico e eleva??o das enzimas hep?ticas; aumento das enzimas renais, sem evid?ncia de les?es macrosc?picas e microsc?picas no tecido renal; leucopenia, com diminui??o significativa de linf?citos e heter?filos; diminui??o da superf?cie de absor??o em fun??o da redu??o do comprimento e largura das vilosidades de todos os segmentos estudados do intestino delgado; presen?a de heter?filos t?xicos. Os efeitos citot?xicos e genot?xicos da aflatoxina B1 foram significativamente reduzidos ou ausentes no grupo piperina associada a aflatoxina B1, sem diferen?a significativa entre o grupo controle e piperina. A ra??o de frangos de corte com 60 ppm de piperina foi segura, promovendo efeito ben?fico tanto nos par?metros zoot?cnicos avaliados, como na sanidade av?cola, por impedir os efeitos t?xicos da aflatoxina B1 em frangos de corte.

Submitted by Verena Bastos (verena@uefs.br) on 2015-09-16T22:20:18Z No. of bitstreams: 1 Disserta??o finalpdf.pdf: 1860889 bytes, checksum: ee87b81fe0a21430df0bad836a2fe39f (MD5) / Made available in DSpace on 2015-09-16T22:20:18Z (GMT). No. of bitstreams: 1 Disserta??o finalpdf.pdf: 1860889 bytes, checksum: ee87b81fe0a21430df0bad836a2fe39f (MD5) Previous issue date: 2013-03-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The present work examined the floristic compositions and structures, and the similarities between seven areas degraded by mechanized diamond mining along the S?o Jos? River Valley (12o34? to 12?36? S - 41o22? to 41?35? W), in the municipality of Len??is, Bahia State, Brazil, after 23 years of abandonment and natural regeneration and compared them to an area of intact gallery forest along the same river. The collections were made between the months of April and December/2011 along 1 x 100 m transects established in each of the seven areas, applying the point method. A previously compiled species list was used to compare the seven recovering areas with an intact riverine forest site. A total of 62 species were encountered, distributed among 53 genera and 26 families. The families demonstrating the greatest species richness were Leguminosae (16), Poaceae (11), Cyperaceae (7), Melastomataceae (6), Malvaceae (4), and Lycopodiaceae (2). The sum of the richnesses of these families represented fully 74% of the total number of species recorded in the present study. In terms of the plant habits, 11 species (15%) were arboreal, 17 (26%) shrubs, 28 (42%) herbaceous, 07(11%) sub-shrubs, and 04 (6%) vines. The species demonstrating the greatest Relative Vigor and Cover were Sebastiania corniculata, Aristida setifolia, and Homolepis aturensis. The slow natural regeneration of these areas was apparently due to the modification of the soil structure by the mechanized mining, as judged by the greater similarity (although low) between the areas regenerating for the longest periods and the intact gallery forest site along the same river as performing solo one little more structured.c / Este trabalho verificou a composi??o flor?stica, estrutura e similaridade entre sete ?reas degradadas pelo garimpo de diamantes no vale do rio S?o Jos? (12o34? e 12?36? S - 41o22? e 41?35? W), munic?pio de Len??is, Bahia, ap?s decorridos 13 anos de registros sobre a regenera??o natural, e comparou com a floresta ciliar do mesmo rio. As coletas foram realizadas entre abril a dezembro de 2011 ao longo de um transecto de 1m x 100m em cada uma das ?reas onde foi aplicado o m?todo de pontos em cada uma das ?reas. Para comparar com o remanescente florestal do entorno, foi usada a lista de esp?cies da floresta ciliar elaborada previamente. Foram amostradas 62 esp?cies distribu?das em 53 g?neros e 26 fam?lias. As fam?lias com maior riqueza de esp?cies s?o Leguminosae (16), Poaceae (11), Cyperaceae (7), Melastomataceae (6), Malvaceae (4), Lycopodiaceae (2). O somat?rio da riqueza destas fam?lias representa 74% do total das esp?cies levantadas neste estudo. Em termos de h?bito foram encontradas 11 (15%) esp?cies arb?reas, 17 (26%) esp?cies arbustivas, 28 (42%) herb?ceas, 07(11%) subarbustivas e 04 (6%) trepadeiras. As esp?cies que mais se destacaram por Vigor Relativo e Cobertura foram Sebastiania corniculata, Aristida setifolia e Homolepis aturensis. A lenta regenera??o natural das ?reas se deve ?s m?s condi??o do solo que apresenta-se modificado pelas a??es do garimpo, tendo sido evidenciada uma maior similaridade, embora baixa, entre as ?reas de maior tempo de abandono que apresentam solo um pouca mais estruturado e o remanescente florestal ciliar do rio S?o Jos?.

Vectorial Boolean functions are used as S-boxes in cryptosystems. To design inequivalent vectorial Boolean functions resistant to known attacks is one of the challenges in cryptography. Verifying whether two vectorial Boolean functions are equivalent or not is the final step in this challenge. Hence, finding a fast technique for determining whether two given vectorial Boolean functions are equivalent is an important problem. A special class of the equivalence called restricted extended affine (REA) equivalence is studied in this thesis. We study the verification complexity of REA-equivalence of two vectorial Boolean functions for some types, namely types I to VI. We first review the verification of the REA-equivalence types I to IV given in the recent work of Budaghyan and Kazymyrov (2012). Furthermore, we present the complexities of the verification of REA-equivalence types I and IV in the case basic simultaneous Gaussian elimination method is used. Next, we present two new REA-equivalence types V and VI with their complexities. Finally, we give the algorithms of each type I to VI with their MAGMA codes.

This thesis focuses on the treatment of insanity in the criminal law and its implications for the concepts and mechanisms of legal accountability. In order to address this issue, I examined the historical background of the insanity defense and five specific cases that demonstrate the complications arising from insanity’s present legal condition. From this case study I drew the conclusion that, because liability to punishment requires particular internal conditions, criminal responsibility is the proper measure of legal accountability for insane persons. Ultimately, my research demonstrated that insanity occupies a unique position in both the theory of crimes and the theory of punishment and that a trial by jury is not the most appropriate way for adjudicating issues of insanity. In each of these spheres, judges consider how mental conditions relate to criminal responsibility and the role that juries play shrinks as the content of guilt shifts to criminal responsibility. For this reason, I conclude that judges are the best candidates for addressing insanity and its effect on criminal responsibility.

Made available in DSpace on 2014-12-17T15:54:51Z (GMT). No. of bitstreams: 1 ClebiaBS.pdf: 2471439 bytes, checksum: 636a341d77ba52afab764309afdb7ef9 (MD5) Previous issue date: 2009-02-19 / The protected areas are created for conservation to nature. The way they associate with the communities and visitors are essential to its objectives are achieved. This study aims to analyze how tourism is developed in the. The Recifes dos Corais Protected Area (APARC) through the identification of the image that tourists and residents have with respect to Parracho of Maracaja? and knowledge of the factors that contribute to a satisfactory visit of tourists. For this was an exploratorydescriptive study with quali-quantitative approach, with 236 tourists and 70 residents of the district of Maracaja?, using questionnaires and interviews. Data analysis techniques were used and percentage of arithmetic, factor analysis and content analysis. The results showed that the importance and significance attributed by residents to Parracho related to economic gains. Information on the APARC and on their standards are not done efficiently and contribute to negatively impact the site. The profile of tourists is not the most appropriate for a protected area. The dimensions of satisfaction to the tourists demonstrated the need for actions to be undertaken by the administration of APARC. Conclude that the image that the residents and tourists have of APARC is not conducive to the image of a protected areas. The tourists are satisfied with the tour in APARC, however, the dimensions of satisfaction are not consistent with the conservation of the site / As unidades de conserva??o s?o criadas para proteger a natureza. A maneira como elas se relacionam com as comunidades vizinhas e com seu visitantes ? determinante para que seus objetivos sejam atingidos. O presente trabalho analisa a forma como ? desenvolvida a atividade tur?stica na ?rea de Prote??o Ambiental dos Recifes de Corais (APARC), atrav?s da identifica??o da imagem que moradores do distrito de Maracaja? e os turistas que visitam o parracho de Maracaja? t?m com rela??o a este, como tamb?m, atrav?s da identifica??o dos fatores que contribuem para uma visita satisfat?ria para turistas que fazem o passeio ao referido parracho. Para isso foi realizado um estudo explorat?rio e descritivo com abordagem qualiquantitativa, com 236 turistas e 70 moradores do distrito de Maracaja?. Os instrumentos de coleta de dados foram o question?rio e o formul?rio pr?-codificado de entrevista padronizada. Para analise dos dados foram utilizadas an?lise descritiva, an?lise fatorial e an?lise de conte?do. Os resultados mostraram que a import?ncia e o significado atribu?dos pelos moradores ao parracho est?o relacionados a ganhos econ?micos. As informa??es sobre a APARC e sobre suas normas n?o s?o feitas de forma eficiente e contribuem para impactar negativamente o local. O perfil dos turistas n?o ? o mais adequado para uma unidade de conserva??o. As dimens?es de satisfa??o, para os turistas mostram a necessidade de a??es a serem desenvolvidas pela administra??o da APARC. Conclui-se que a imagem que os moradores e turistas t?m da APARC n?o ? condizente com a imagem que deve ter uma unidade de conserva??o. Os turistas mostraram-se satisfeitos com o passeio na APARC, contudo, as dimens?es de satisfa??o encontradas n?o s?o condizentes com a conserva??o do local

Made available in DSpace on 2014-12-17T15:54:54Z (GMT). No. of bitstreams: 1 IltonAS_DISSERT.pdf: 1448193 bytes, checksum: f8d58bca2c062ed6c6946d11628a4495 (MD5) Previous issue date: 2010-02-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The purpose of this paper is to identify and analyze the main environmental impacts taking place in the permanent preservation areas located in the Cear?-Mirim River estuary/RN and its surrounding areas. For that, the study was divided in two chapters, structured in the shape of a scientific paper. The first is a theoretical proposition on the occupation of the APPs in light of the discussion of the geographic space and the sustainability of the development, where a bibliographical review was made emphasizing the production of the geographic space, the sustainability of the development and the permanent preservation areas. In the second chapter, from a systemic approach, geoprocessing techniques and the adaptation of the Pressure-State- Response Indicators System were used, the latter being developed by the Organization for Economic Co-operation and Development OECD. Therefore, from the methodological approaches used, it's been observed that the use of permanent preservation areas for purposes other than those determined in the legislation and, mainly guided by capitalist interests, leads to the environmental degradation of these areas, fact which has been confirmed in the empiric study conducted in the second chapter, where it was possible to identify a series of environmental impacts such as: deforestation of mangrove and riparian vegetation for carciniculture implantation, the devastation of dune areas caused by the urban expansion in the coastal stretch and siltation of fluvial channel / O objetivo desta disserta??o ? identificar e analisar os principais impactos ambientais ocorrentes nas ?reas de preserva??o permanente localizadas no estu?rio do Rio Cear?-Mirim/RN e suas ?reas adjacentes. Para isto, o estudo foi dividido em dois cap?tulos estruturados em forma de artigo cient?fico. O primeiro ? uma proposi??o te?rica sobre a ocupa??o das APPs a luz da discuss?o do espa?o geogr?fico e da sustentabilidade do desenvolvimento, onde foi feita uma revis?o bibliogr?fica enfatizando a produ??o do espa?o geogr?fico, a sustentabilidade do desenvolvimento e as ?reas de preserva??o permanente. No segundo cap?tulo, a partir de uma abordagem sist?mica foram utilizadas t?cnicas de geoprocessamento e a adapta??o do Sistema de Indicadores Press?o-Estado Resposta, desenvolvido pela Organization for Economic Cooperation and Development OECD. Desta forma, a partir das abordagens metodol?gicas utilizadas, observou-se que a utiliza??o de ?reas de preserva??o permanente para fins diferentes do que est? posta na legisla??o e, principalmente guiada por interesses capitalistas, leva a degrada??o ambiental destas ?reas, fato confirmado no estudo emp?rico realizado no segundo cap?tulo, onde foi poss?vel identificar uma s?rie de impactos ambientais como: desmatamento de vegeta??o de mangue e de mata ciliar para implanta??o da carcinicultura, a devasta??o de ?reas de dunas provocada pela expans?o urbana na faixa litor?nea e assoreamento de canal fluvial