No description available.

Adult mammalian <a></a><a>cardiomyocytes </a>(CMs, or heart muscle cells) have little, if any, ability to proliferate in response to injury, and after myocardial infarction this defect underlies the poor regenerative ability of human hearts. In contrast, early stage of CMs (such as fetal CMs) still have some ability to proliferate, and we seek to identify novel gene regulators as potential therapeutic targets for heart regeneration. Here we use human pluripotent stem cells (hPSCs) as an in vitro human model to investigate the roles of emerging long non-coding RNAs (lncRNAs), with the lengths of over 200 nucleotides are able to be transcribed but not translated into protein, for heart regeneration. With public available RNA-sequencing data, we identified several human genes, including lncRNAs, that are highly enriched in fetal CMs. We generated targeted gene knockout hPSC lines using CRISPR/Cas9-mediated genome editing and will use them to study the roles of selected genes in regulating CM proliferation. To identify more therapeutic targets, we also generated a fluorescence ubiquitination cell cycle indicator (FUCCI) reporter cell line that express either green (indicating dividing cells) or red fluorescence (indicating non-dividing cells), on which we’ll perform unbiased genome-wide screening to identity genes that regulate CM proliferation. High-throughput chemical screening will also be performed on FUCCI reporter lines to identify potential therapeutic drugs for heart regeneration.

A content analysis was conducted to determine the amount and type of causal explanation included in coverage of the 1992 Los Angeles riots in The New York Times, the Los Angeles Times and The Christian Science Monitor. The data were analyzed to determine whether causal explanations were primarily societal or individual/situational. The primary purpose was to examine whether the press has altered its reporting techniques since the Kerner Commission report criticized the narrow, descriptive-based reporting of the 1960s riots. Study results indicate riot coverage was predominantly descriptive and similar in content to that detailed by the Kerner Commission. The most frequently cited cause was the triggering event, the Rodney King acquittal verdict.

There is currently very few information about the transaltion of linear cytoplasmatic plasmids occured in yeast cells Kluyveromyces lactis. However, there is a relatively well developer information about their transcription apparatus. A study of transkript linear plasmids revealed an atypical organization at the 5ʼ end. Those ends contain nontemplate polyadenylation and they are missing the N7 methylguanosine hat. Because of the presence of this structure, which is localized at 5ʼend of plasmids specific mRNA, raised a question regarding the iniciation of the translation. The present thesis is focused on the preparation of reporter systém suitable for studying the influence of a number of the nontemplate adenosins, which were added at the 5ʼ ends of mRNA linear plasmids. The frist step was making a construction of dual yeast cell plasmids carring two reporters genes, which are under the controle of two different promoters. After a successfull construction, the aktivity of promoters TEF1 and PGK1 was measured, whereby the promoter TEF1 proved twice stronger. The transcription start site of both promotor was determined. The second step was the construction of a reporter system directly in yeast cell plasmid pGKL. Reporter genes were under the controle of two promoters originating from the pGKL...

The COVID-19 pandemic has been one of the most impactful crises in recent times, with severe implications for people around the globe. Since its beginnings in late 2019, the crisis has been closely monitored by news media, which function as one of the primary information sources for the public. This study looks at the many different challenges the COVID-19 pandemic has brought upon journalism and how journalists reporting on the pandemic have been experiencing them. The project is based on the central premise that journalism holds an especially important position within the socio-political make-up of democratic societies, and employs the social responsibility theory of the press to argue that journalists should act with responsibility to the public in mind and stick to generally agreed on professional values. It further assumes, based on previous research, that a crisis calls for increased social responsibility but at the same time creates challenges for journalists, which put their ability to adhere to established standards at risk. To determine, which challenges of this sort journalists have experienced during the COVID-19 pandemic and how these have manifested, a series of semi-structured interviews has been conducted with German journalists who have been involved in reporting on the pandemic. The results show that the COVID-19 pandemic has caused major disruptions to newsrooms and has proven challenging to journalists in various ways regarding their work routines, physical safety, and emotional labour, which at times even put their ability to perform expected professional roles in jeopardy. This project adds to a growing body of research on COVID-19 from a journalism studies perspective, which can help determine grievances for the profession that need to be addressed and provide points of departure on how to deal with and prepare for future crises.

No description available.

Thesis (MA)--Stellenbosch University, 2012. / Includes bibliography / ENGLISH ABSTRACT: The onset of democracy in South Africa in 1994 was accompanied by the rise in bids for, and the hosting of sports mega-events so as to accomplish national interests and goals. This was done with the purpose of rebranding the South African image to the international community through national and international campaigns that sought to highlight the country’s aspirant status as a rainbow nation and its pan-Africanist ideals. This study investigates how, as host for the 2010 FIFA World Cup™, South Africa was reported on by two international online media newspapers, The New York Times (United States of America (USA)) and the Guardian (United Kingdom (UK)). The aim is to address an understudied aspect of South Africa’s hosting of the 2010 FIFA World Cup™ by reflecting systematically on the tone and content of international media portrayals of the event, both before and during the tournament. The study has two focuses. Firstly, it considers the motives for South Africa’s bid to host the 2010 FIFA World Cup™. Secondly, it appraises the content and nature of reporting in the two overseas newspapers. The study uses a mix of secondary and primary sources, which include academic journals, books, websites, newspaper articles and government and the FIFA websites. The findings of this study suggest that the bid to host the 2010 FIFA World Cup™ was based on the country’s positive experience from hosting previous sports mega-events. Additionally, South Africa wanted to showcase its commercial maturity, its development of physical infrastructure, and the presence of human skills. The motives underpinning the bid aimed at dispelling and challenging international misconceptions of the African continent. The novelty of an African country bidding to stage and hosting a sport mega-event such as the FIFA World Cup™ resulted in the country gaining extensive international media coverage from The New York Times and the Guardian. The qualitative and quantitative content analysis from these two newspapers yielded some commonality and recurrence of words such as: “stadium”, “tickets”, ‘vuvuzela”, “crime”, and “security”. The differences between the two newspapers were minimal, supporting the liberal-pluralist theoretical claim that the media acts as an agenda setter, and in line with the Marxist theory of the ideological role of the media. Media coverage of sports mega-events is important and influential in determining the way in which the host country is branded, and future studies are necessary to address the / AFRIKAANSE OPSOMMING: Die koms van demokrasie in Suid-Afrika in 1994 het gepaard gegaan met die toename in tenders en die gasheerskap van megasportgebeure om nasionale belange en doelwitte te bereik. Die doel was die herposisionering van die Suid-Afrikaanse beeld in die internasionale gemeenskap deur middel van nasionale en internasionale veldtogte wat daarna gestreef het om die land se reënboognasiebeeld en sy pan-Afrikanistiese ideale te beklemtoon. Hierdie studie ondersoek hoe Suid-Afrika, as gasheer vir die 2010 FIFA Wêreldbeker, deur twee internasionale aanlynmediakoerante, The New York Times (Verenigde State van Amerika) en die Guardian (Verenigde Koninkryk) uitgebeeld is. Die doel is om die meer onverkende aspekte van Suid-Afrika se gasheerskap onder oë te neem, en voorts om sistematiese peiling te doen van die toon en inhoud van internasionale media-uitbeeldings van die sport gebeurtenis. Die studie het twee fokuspunte. Eerstens word ondersoek ingestel na die motiewe van Suid-Afrika se bod om die 2010 FIFA Wêreldbeker aan te bied. Tweedens beoordeel dit die inhoud en aard van verslaggewing in die twee oorsese koerante. Die studie gebruik ’n mengsel van sekondêre en primêre bronne, insluitend akademiese tydskrifte, boeke, webwerwe, koerantberigte en die regering en FIFA se webwerwe. Die bevindinge van hierdie studie beklemtoon dat die motiewe van Suid-Afrika se bod om die 2010 FIFA Wêreldbeker aan te bied, gegrond was op die bewese positiewe prestasierekord wat die land as gasheer in vorige megasportgebeure opgebou het. Voorts wou Suid-Afrika sy kommersiële volwassenheid, die ontwikkeling van fisiese infrastruktuur, en die teenwoordigheid van mensvaardighede ten toon te stel. Die motiewe vir die bod was ook daarop gemik om internasionale wanopvattings oor die Afrika-vasteland uit te daag en uit die weg te ruim. Die ongekendheid van die aanbied van ’n megasportgebeurtenis soos die FIFA Wêreldbeker deur ’n Afrikaland, het daartoe gelei dat die land uitgebreide internasionale mediadekking in The New York Times en die Guardian geniet het. Die kwalitatiewe en kwantitatiewe inhoudontleding het getoon dat daar ’n mate van gemeenskaplikheid en herhaling van woorde was, soos: “stadium”, “tickets”, “vuvuzela”, “crime” en “security”. Die verskille tussen die twee koerante was minimaal en ondersteun liberaal-pluralistiese teorie wat die media as ’n agenda steller uitwys. Dit ondersteun ook Marxistiese teorie oor die ideologiese rol van die media. Mediadekking van megasportgebeure is belangrik en invloedryk in die bepaling van die manier waarop die gasheerland as handelsmerk voorgestel word, en toekomstige studies is nodig om die onderbestudeerde aspekte van die 2010 FIFA Wêreldbeker ™ te ontleed. Dit sluit onder andere in, ontleding van die langtermyn ekonomiese, politieke en maatskaplike nalatenskappe van so ’n gebeurtenis.

Lam Kwan Heung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 135-137). / Abstracts in English and Chinese; appendices in English with some Chinese. / Abstract --- p.ii-iv / Acknowledgement --- p.v / Table of Contents --- p.vi / Chapter Chapter 1 --- Introduction --- p.1-6 / Chapter Chapter 2 --- Literature Review --- p.7-22 / Chapter Chapter 3 --- Research Methods --- p.23-35 / Chapter Chapter 4 --- An Overview on Hainan --- p.36-46 / Chapter Chapter 5 --- Framing of Hainan --- p.47-92 / Chapter ´Ø --- International law and order / Chapter ´Ø --- U.S. peacekeeping surveillance / Chapter ´Ø --- Victimized U.S / Chapter Chapter 6 --- Framing of China --- p.93-125 / Chapter ´Ø --- China's skewed media / Chapter ´Ø --- China as a problematic communist state / Chapter ´Ø --- China as a secretive military power / Chapter ´Ø --- China's aggression towards Taiwan / Chapter ´Ø --- China's buying off U.S. politicians / Chapter Chapter 7 --- Conclusion --- p.126-134 / Bibliography --- p.135-137 / Appendices --- p.138-182

The thesis presents a contribution to the topic of critical examination of various ways to evaluate and compare the level of press (media) freedom in countries throughout the world. It analyses the process of presentation the conclusions of press freedom indices to the public. The analysis is guided on two levels. First, the form in which the press freedom indices are published is investigated. And second, the media reflection of these publications is examined. The starting-point of this analysis focuses on theoretical view on various definitions of press freedom. First part of the thesis therefore provides the summary of important fields that should not be forgotten by any conceptualization of press freedom. Second chapter deals with the problems connected to evaluations and comparisons of press freedom in general and then in particular concerning the Freedom of the Press index by Freedom House and World Press Freedom Index by Reporters Without Borders. In the second part of the thesis, Czech media reflection of the two concerned indices is analyzed. First, the specifics of the Czech media landscape are dealt with. Next, the content analysis is applied that should approach the way Czech media use the data provided by the indices. The thesis then concludes the most serious weaknesses in the process through...

L'organisation orientationnelle bio-moléculaire des lipides et des protéines dans la membrane plasmique constitue un facteur important dans les processus biologiques au cours desquelles les fonctions peuvent être reliées aux mécanismes d'orientation et d'organisation. Le concept de séparation transitoire des phases à l'échelle nanométrique dans les domaines ordonnés et désordonnés, aussi appelé « radeau lipidique », est maintenant largement accepté. De plus, les domaines ordonnés contiennent des protéines de signalisation, ce qui souligne l'importance des séparations de phase au cours des processus de signalisation. Dans cette thèse de doctorat, nous avons étudié l'ordre orientationnel moléculaire de la protéine de signalisation MHC Class I et de reporters lipidiques tels que di-8-ANEPPQ et DiI(C18). Nous avons étudié l'ordre orientationnel moléculaire de la protéine de signalisation MHC Class I et des reporters lipidiques par imagerie d'anisotropie de fluorescence résolue en polarisation. Nous avons observé l'influence du cytosquelette d'actine sur l'ordre orientationnel moléculaire de la protéine MHC et des reporters lipidiques dans la membrane plasmique. De plus, nous avons trouvé que l'ordre orientationnel moléculaire des reporters dépend de la morphologie cellulaire. Nous avons examiné les plis membranaires en modifiant la forme des cellules de façon mécanique ou pharmacologique. / Biomolecular orientational organization of lipids and proteins in the plasma membrane is a crucial factor in biological processes where functions can be closely related to orientation and ordering mechanisms. The concept of transient nanosized phase separations in ordered and disordered domains, called "lipid rafts" is now widely accepted. Furthermore, the ordered domains are enriched in signaling proteins, which highlights the crucial impact of phase separation during the signaling processes. While this field has been so far largely addressed by studying the translational diffusion behavior of membrane proteins and lipid reporters by Single Molecule Tracking (SMT) or Fluorescence Correlation Spectroscopy (FCS), only little is known about the orientational behavior of signaling proteins and lipid reporters in the plasma membrane. In this PhD thesis we investigated the molecular orientational order of the signaling molecule MHC Class I protein using fluorescence anisotropy imaging as well as of lipid reporter di-8-ANEPPQ using polarization-resolved fluorescence imaging. Fluorescence anisotropy imaging requires a fluorescent label rigidly attached to the system under study, able to report its orientational order behavior. Thus, MHC Class I protein has been successfully labeled in a rigid way. We analyzed the orientational order of MHC Class I protein quantitatively in the endomembrane and plasma membrane and we found that the orientational order of MHC Class I protein in both membranes depends primarily on the maturation state of the protein and its interaction with the cytoskeleton.