The flood of 2000 caused extensive changes within the riparian landscape of the Sabie River, Kruger National Park (KNP). Changes within the riparian landscape and the removal of vegetation resulted in considerable changes in riparian vegetation characteristics. Open patches created by the flood served as a template for the establishment of new species and the regeneration of existing species, which consequently resulted in a patch mosaic. This memorable event encouraged an investigation into the response of the Sabie River ecosystem to the memorable Large Infrequent Disturbance (LID). Riparian ecosystems are driven by varying combinations of environmental factors, such as water availability, disturbance, herbivory, fire and river morphology. This complexity depicts unique vegetation structure and assemblages of associated plant species. The lack of sufficient knowledge on the role of riparian vegetation in the health assessment of surrounding ecosystems along semi-arid rivers prompted the establishment of the Kruger Rivers Post Flood Research Program (KRPFRP). Research conducted through this monitoring program four years after the 2000 flood, revealed no significant changes in the species composition, although the location and density of many common riparian species have been changed. There was a decrease in species density across the macro channel floor (MCF) and an increase in species density across the macro channel bank (MCB). Furthermore, it was reported that the flood altered the distribution of height classes across the macro channel. In general the riparian vegetation was shorter and bushier four years post-flood. These studies furthermore illustrated that the tree to shrub ratio did not change drastically from pre-flood conditions, although a decrease in the number of shrub individuals was reported. The research presented in this dissertation was designed to further explore changes in woody species composition and structure along the Sabie River, KNP at a post flood temporal interval, i.e. between the last survey in 2004 (by the KRPFRP) and 2010. For data compatibility, the sampling and analytical approach of this study conforms to the approach followed by the KRPFRP. Data were sampled within four preselected belt-transects that form part of the larger KRPFRP. All established woody individuals were counted and measured within each contiguous 10 m x 30 m plot within each of the four belt-transects. Log transformed species composition data were analysed through the application of the Bray Curtis dissimilarity index in combination with Ward’s method of clustering. Statistical significant differences between clusters were tested through the application of the Fisher’s exact relationship test. The MIXED Procedure or PROC MIXED model was used to investigate change within the vegetation structural data. Results obtained through the various analytical methods broadly support the findings of the KRPFRP. No significant change in woody species composition could be detected between 2004 and 2010. However, a change in the density (increase and decrease) of certain species across the MCB and MCF was revealed. Species richness and density increased significantly on the MCF oppose to small changes on the MCB. A significant increase in the total number of shrubs on the MCF contributed to an overall increase in woody density for the entire study area between 2004 and 2010. Shrubs therefore remained the most dominant growth form in both sampling years. Trees decreased across the MCB although the total number of established trees remained unchanged between 2004 and 2010. Riparian vegetation structure is directly linked to species assemblages, hence the continued dominance of shrub species along the Sabie River in the KNP The Sabie River riparian landscape is therefore still characterised by short and multi-stemmed woody individuals ten years after the LID. / Thesis (MSc (Environmental Sciences))--North-West University, Potchefstroom Campus, 2013

xviii, 105 p. : ill. (some col.) / Restoration of riparian ecosystems in semi-arid riparian ecosystems requires an understanding of geomorphic, hydrologic and biologic factors and how they relate to vegetation. Such an understanding allows prioritization of restoration projects and avoidance of activities that are either unnecessary or likely to fail. In this dissertation I examined a suite of factors controlling distribution of vegetation types in the Sprague Basin, OR, and used those factors to predict potential for natural recovery. Factors ranged from basin-wide (e.g. floodplain width and slope) to local (e.g. topography, hydrology and soil texture). Results of historical analysis and photographic mapping showed that basin-wide vegetation types have remained generally stable since the early 1940s and that wide floodplains have been without woody vegetation since the late 1800s. The most prevalent changes in floodplain vegetation due to land use included reduction of shrub cover in moderately wide floodplains and associated increases in herbaceous vegetation. Soil moisture conditions were studied using piezometers and nested clusters of soil moisture tension meters. The interrelations among soil texture, elevation and distance from the channel, and vegetation (herbaceous and woody) characteristics in the riparian zone were examined along 75 transects using a generalized additive model for non linear factors and Hurdle analysis for abundance data. On the Sprague mainstem, fine soils with high recession rates supported abundant shrubs, while on the Sycan (Sprague tributary) coarse soils with readily available moisture and greater subsurface water movements supported abundant shrubs. Habitats in the Sycan were well colonized with new shrub seedlings though long term persistence was unlikely. Results show that riparian shrubs are unlikely to influence stream shade or bank stability on the mainstem Sprague whether they germinate naturally or are planted through restoration efforts, as shrubs near the channel are unlikely to persist long term. In the Sycan, germination and persistence are more likely than on the Sprague, though risks of predation, trampling from grazers, and fluvial action will be constant threats to near-channel shrubs. Results emphasize the need to understand factors controlling vegetation prior to restoration in any basin or stream segment. / Committee in charge: Patricia F. McDowell， Chairperson； W. Andrew Marcus， Member； Patrick Bartlein， Member； Scott Bridgham， Outside Member

This work was done in the caatinga dominium in an area of 220 hectares of forest located in the Serra dos Macacos Canyon, municipality of Tobias Barreto, in the period 2009 to 2010. The aim was to diagnose the structure, composition and coverage of vegetation for conservation purposes and conduct a survey dendrochrological trees species. We sampled about 12000 m2 of forest, divided into three plots of 4000 m2 in order to determine the phytosociological parameters of the community tree-shrub canopy and herbaceous understory, species richness and dendrochronological survey of tree species forming rings growth. The plots were distributed in a transect of 1500 m with reference to the creek bed at the Burrow Monkeys at altitudes of 360, 420 and 500 meters to sea level. The trees of the Canyon have two defined strata of canopy and understory shrub and vegetation type with the formation of riparian vegetation, which closed canopies and structured with individuals up to 25 m high and trunk circumference (CAP) of up to 160 cm. The herbaceous layer in the understory has a rich diversity of bromeliads (8) species recorded in the Atlantic. The richness of the floristic composition of 93 species were identified. The vertical structure of vegetation in relation to height, CAP showed strong adjustment of the curves of distribution and abundance "J" reversed indicating that the community is present in mature or stable stage of regeneration. The phytosociological study determined the dominance of the family Fabaceae (Leguminosae - Caesalpinioideae, Faboideae and Mimosoideaee) comprising 17 species of caatinga. The structural patterns of the populations assessed by the Index of Importance Value Index Phytosociology and tree cover in the community of Canyon had a distance-dependent spatial distribution of stream and associated with the behavior of canopy opening, reinforced the idea of riparian vegetation with semideciduous. The analysis of tree cover and shrubs using the GLA (Gap Light Analyzer) demonstrated that semidecidual ranged 30-70% open canopy in the dry season or month in February and a canopy closure of 80 to 90% in September, post-rain season. Prospecting dendrochronological community found that 10 species in the Serra dos Macacos have potential in the formation of growth layers. The analysis of a population of 86 individuals Pseudobombax marginatum (Malvaceae), with the objective of determining the age of the trees through the rings of growth through cross-dating series, we obtained a chronology of 83 sets of growth rings 51 individuals (2353 rings P. marginatum), yielding a value of inter-correlation of 0.449, with mean sensitivity of 0.522, while the number of missing rings was less than 3.0%. The principal component analysis (PCA) indicated that the first components explained 64.58% by 61.23% and ARSTAN chronology Residual variance (ANOVA) of plant growth by estimating the chronological age of the trees up to 62 years in the community. The formation of the rings of annual growth is positively correlated with precipitation for the current year (between April and July) with the temperature of the previous year (September to November) and is associated with deciduous species showed that in the dry season. The behavior of tree growth of revealed significant variations in the rate of average annual increment of 2.15 mm / year, and the population mean age was 27 years. The analysis of climatic parameters was established through a series of 96 years (1914-2010) for precipitation and temperature series of about 50 years (1967-2007) obtained from the Meteorological Station of Samambaia, located at 9 km near of the Canyon. Temperature data were calibrated with data obtained from the SST NOAA (National Oceanic and Atmospheric Administration). Temperature data location had a temperature range above 9ºC, different from the 6ºC as described for the NEB (Northeast of Brazil) and showed marked seasonal climate with increased precipitation and lower temperatures during the months from March to July and rainfall lower and higher temperatures between September and February. The climate for the region of Tobias Barreto proved to be mainly influenced by anomalies in sea SST (surface temperatures). This study establishes for the first time a model for predicting age of tree species associated with the climate in the Northeast of Brazil. Species richness and occurrence of rare species in this enclave in the caatinga forest contributes significantly to the goals of preservation and conservation of this biome. / Este trabalho foi realizado em domínio da caatinga numa área de 220 hectares de floresta localizada no Canyon da Serra dos Macacos, Município de Tobias Barreto, no período de 2009 a 2010. O objetivo do estudo foi diagnosticar a estrutura, composição e cobertura da vegetação para fins de conservação e realizar uma prospecção dendrocrológica das espécies arbóreas. A análise dos parâmetros climáticos foi estabelecida através de séries de 96 anos (1914 a 2010) para a precipitação e séries de temperatura de cerca de 50 anos (1967 a 2007). Os dados de temperatura foram calibrados com dados das Temperaturas da Superfície do Mar (TSM) obtidos através do NOAA (National Oceanic and Atmospheric Administration). Os dados de temperatura local tiveram uma amplitude térmica superior a 9ºC, diferente dos 6ºC descritos para o NEB (Nordeste Brasileiro) e apresentaram acentuada sazonalidade climática tanto para a precipitação quanto para a temperatura. O clima para a região de Tobias Barreto mostrou-se, principalmente, influenciado pelas anomalias nas TSM. Foram amostrados cerca de 12000 m2 de floresta divididos em três parcelas de 4000 m2. As parcelas foram distribuídas em um transecto linear de 1500 metros tendo como referência o leito do riacho Macacos na Toca da Onça nas altitudes de 360, 420 e 500 metros ao nível do mar. Foram determinados os parâmetros fitossociológicos da comunidade arbórea arbustiva do dossel e herbácea do sub-bosque, riqueza de espécies e relações dendrocronológicas de espécies arbóreas formadoras de anéis de crescimento. A vegetação arbórea do Canyon possui dois estratos definidos de dossel e sub-bosque arbustivo e fitofisionomia com formação de vegetação ripária, com indivíduos de até 25 m de altura e Circunferência do Tronco (CAP) de até 160 cm. O estrato herbáceo no sub-bosque possui uma rica diversidade de bromélias (8) espécies de ocorrência na Mata Atlântica. A riqueza da composição floristica foi de 93 espécies. A estrutura vertical da vegetação em função da altura e CAP apresentou curvas de distribuição e abundância em J invertidos indicando que a comunidade atual está em estágio maduro ou estável de regeneração. O estudo fitossociológico determinou a dominância da família Fabaceae (Leguminosae - Caesalpinioideae, Mimosoideaee e Faboideae) compondo 17 espécies de caatinga. Os padrões estruturais das populações avaliados pelo Índice de Valor de Importância Fitossociologica e Índice de Cobertura Árbórea da comunidade do Canyon apresentaram uma distribuição espacial dependente da distância do riacho e associada ao comportamento de abertura do dossel, caracterizando a vegetação como riparia semidecidual. A análise da cobertura arbóreaarbustiva utilizando o Gap Light Analizer (GLA) demonstrou que a semidecidualidade, variou de 30 a 70% de abertura do dossel no mês fevereiro (estação seca) e um fechamento do dossel entre 80 a 90% no mês de setembro (estação pós-chuva). Na prospecção dendrocronológica da comunidade constatou-se que 10 espécies presentes na Serra dos Macacos formam camadas de crescimento. A análise de uma população de 86 indivíduos de Pseudobombax marginatum (Malvaceae), através da datação-cruzada das séries radiais de anéis de crescimento, resultou em uma cronologia com 83 séries para 51 indivíduos (2353 anéis), com valor de inter-correlação de 0,449 e sensibilidade média de 0,522, enquanto a quantidade de anéis ausentes foi inferior a 3,0%. A análise de componentes principais (PCA) explicou 64,58% para a cronologia Arstan da variância (ANOVA) do crescimento das plantas estimando a idade cronológica das árvores em até 62 anos na comunidade e 61,23% para a cronologia Residual. A formação dos anéis de crescimento anuais relacionou-se positivamente com a precipitação do ano corrente (entre abril e julho) e com a temperatura do ano anterior (setembro a novembro), bem como, com a caducifólia das espécies no período seco. A taxa de incremento médio anual das árvores de P. marginatum foi de 2,15 mm/ano, sendo que a população apresentou idade média de 27 anos. Este estudo estabelece pela primeira vez um modelo de predição de idade de espécie arbórea associado à sazonalidade climática no Nordeste do Brasil. A riqueza de espécies e ocorrência de espécies raras neste enclave de floresta na caatinga contribui significativamente aos objetivos de preservação e conservação deste bioma.

Considering the degradation scenery of riparian vegetation along the São Francisco River. This work discusses through the evaluation of forest species with potential use in soil in a Natural Engineering techniques, the floristic rebuilding of the riverbank. The first chapter consists of the presentation of the problem of bank erosion and the necessary restoration of riparian vegetation in the riparian agroecosystems associated with soil in natural engineering techniques as a mitigation measure of this form of environmental degradation. The second chapter consists of the proposed riparian restoration, by assessing the development of seedlings of forest species on the right bank of the São Francisco River, an important determinant in the choice of species to be used in biotechnical planned for the waterfront. The third chapter tests shrubs / trees to determine which species that have potential for biotechnical composition of soil in natural engineering techniques, assessing morphological species, so that these can contribute in reducing the erosion of river banks. / Diante do cenário de degradação da vegetação ciliar nas margens do Rio São Francisco. O presente trabalho objetiva discutir por meio de avaliação de espécies florestais com potencial uso em técnicas de Engenharia Natural, a recomposição florística da margem do rio. O primeiro capítulo consiste na apresentação da problemática da erosão marginal e a necessária recomposição da vegetação ciliar nos agroecossistemas ribeirinhos associado às técnicas de Engenharia Natural como medida mitigadora dessa forma de degradação ambiental. O segundo capítulo consiste na proposta de recomposição ciliar, através da avaliação do desenvolvimento de mudas de espécies florestais na margem direita do rio São Francisco, importante na determinação nas escolhas das espécies a serem utilizadas nas biotécnicas planejadas para a margem do rio. O terceiro capítulo testa espécies arbustivas/arbóreas a fim de determinar quais espécies que possuem potencial biotécnico para composição de técnicas de Engenharia Natural, avaliando dados morfológicos das espécies, de modo que esses possam contribuir na redução do processo erosivo das margens fluviais

Este trabalho avaliou se os instrumentos legais do licenciamento ambiental da extração de areia contribuem para a recuperação das Áreas de Preservação Permanente (APP), além disso, investigou as condições e os recursos dos órgãos envolvidos e o grau de informação dos proprietários. A área de estudo foi a Bacia Hidrográfica do Rio Corumbataí, localizada no Estado de São Paulo. A estratégia de estudo se baseou: a) na análise dos processos de licenciamento ambiental e mineral dos 36 empreendimentos existentes na bacia; b) na análise de campo dos plantios em APP de 21 portos de extração de areia de leito de rio; c) em entrevistas com 15 empreendedores e 15 funcionários dos cinco principais órgãos envolvidos. Foi verificado que a maioria dos órgãos envolvidos apresentam alguma dificuldade na atribuição de suas funções dentro do licenciamento, o que reside principalmente da carência de recurso humano, além da carência de recursos financeiros e materiais e de condições de preparo dos técnicos envolvidos. As irregularidades ambientais e legais dos portos de areia são função de uma soma de fatores, entre eles, a falta de informação e negligência dos empreendedores e a lentidão e os elevados custos do licenciamento ambiental e mineral. A avaliação final mostrou que 52% dos plantios podem ser classificados como ruins, 38% como regulares e somente 10% puderam ser considerados como bom e ótimo. Na maioria das vezes, as técnicas de reflorestamento utilizadas não atenderam aos critérios mínimos para o restabelecimento da estrutura e da função de uma floresta. Foi evidenciado ainda uma situação preocupante de perda de biodiversidade, uma vez que a maioria dos plantios e projetos de reflorestamento possui baixa diversidade em espécies e uma tendência à homogeneidade florística quando comparado com áreas naturais. Chama a atenção o insucesso dos plantios efetivados. Grande parte deles apresentou alta mortalidade, baixa densidade, baixa cobertura do solo por sombreamento da copa, fisionomia florestal sem estrutura vertical bem definida, serapilheira ausente ou escassa e regeneração ausente ou de baixas quantidade e qualidade. Os projetos de recuperação por reflorestamento não são satisfatoriamente cumpridos pelos empreendedores e a maioria mostrou uma listagem florística idêntica a de outros projetos. Além disso, se fossem implementados, na sua maioria, não garantiriam a manutenção da biodiversidade natural das matas ciliares. De acordo com os resultados, pode-se inferir que os atuais instrumentos legais do licenciamento ambiental dos portos de areia não estão contribuindo para a recuperação das APP da Bacia do Rio Corumbataí. Entretanto, o licenciamento ambiental da atividade parece estar contribuindo com a conservação das matas naturais ainda existentes na bacia, em função da atual política ambiental de não desmatamento de novas matas em APP. / The purposes of this work were to evaluate whether the environmental licensing legislation for sand mining contributes to the recovery of the riparian vegetation of the Permanent Preservation Areas (river buffer zones), as well as the conditions and resources availability of the involved agencies, and the land owners’ information level. The study area was the Corumbatai River basin, located in the State of São Paulo. The study strategy involved: a) the analysis of the environmental licensing procedures of the 36 existing mining sites in the river basin; b) field analysis of the seedlings growth in the revegetated areas in the 21 sand mining sites; c) interviews with 15 entrepreneurs and 15 officers of the five main agencies involved. It was observed that most agencies have problems with their own attributions in the licensing process, mainly owing to human resource deficiencies, lack of financial and material resources, and poor conditions for the adequate technical capacitating of the responsible officers. The environmental and legal irregularities of the sand mining sites are due to several factors, such as entrepreneurs’ misinformation and negligence, slowness and high costs involved in the environmental and mineral licensing. A final evaluation showed that 52% of the revegetation areas could be classified as bad, 38% as regular, and only 10% could be considered good or excellent. Generally, the revegetation techniques used did not meet the minimal criteria for reestablishing the riparian forest structure and function. A worrying situation regarding biodiversity loss was also evidenced, since most of the revegetation projects presented low species diversity and a tendency to floristic homogeneity, as compared to what is observed in natural areas. Most of the revegetated areas showed high mortality rate, low density, little soil cover due to canopy shading, poorly defined vertical structure, absense or inadequate litter and absence or low regeneration quantity and quality. Revegetation projects are not succesfully accomplished by the entrepreneurs and most of them showed an identical floristic list of species to those found in previous projects. Moreover, if such projects were implemented, they would probably not guarantee the maintenance of the natural biodiversity of riparian forests. According to the results, it can be inferred that the current environmental licensing legislation of sand mining sites are not contributing to the recovery of the riparian vegetation in the Corumbatai River basin. However, the environmental licensing of the sand mining activity may seem to be contributing to the conservation of the remaining natural forests in the basin, due to the current environmental policy that prohibities the cutting of riparian forests.

Los corredores riparios en ambientes semiáridos mediterráneos son ecosistemas de gran biodiversidad y complejidad. En ellos confluyen una gran variedad de perturbaciones naturales capaces de crear un mosaico espacial y temporal con pocos paralelos en otros ecosistemas. Sin embargo, a pesar de su valor, los ecosistemas riparios están amenazados debido a los altos niveles de intervención humana. La construcción de presas (y la consecuente manipulación del caudal) está considerada como la perturbación humana más importante que se cierne sobre ellos. Esta tesis ha tenido como objetivo analizar el ecosistema ripario, abarcando diferentes escalas espaciales y temporales, y centrándose en los factores que influyen en la distribución, estructura, composición, calidad y dinámica de su vegetación, tanto en tramos naturales como hidrológicamente alterados de ríos mediterráneos, concretamente de la Demarcación Hidrográfica del Júcar (Este de España). Para lograr este objetivo, se han hecho los siguientes aportes a la investigación: A) Determinar los patrones de distribución de especies leñosas riparias en el gradiente transversal de la ribera y definir grupos de especies con respuesta similar a las condiciones físicas del hábitat; B) Comparar la respuesta de dichas especies en tramos naturales y alterados hidrológicamente; C) Definir curvas de respuesta y gremios hidrológicos de especies en tramos con régimen natural; D) Establecer los principales factores que determinan la calidad del hábitat ripario y fluvial y los patrones longitudinales de la composición florística, así como de las características del hábitat fluvial en un segmento hidrológicamente alterado; E) Describir los cambios espacio-temporales en la estructura y complejidad de la vegetación, y los cambios temporales en el régimen de caudales del corredor ripario de un tramo hidrológicamente alterado. Los objetivos anteriores fueron abordados con diferentes metodologías que implicaron la combinación de diversas fuentes de información y un esfuerzo importante en la toma de datos en varios lugares de estudio de los ríos Cabriel, Mijares y Serpis. Para cumplir con los objetivos A y B, se llevaron a cabo un muestreo de suelos y un muestreo georreferenciado de vegetación a través de transectos transversales al cauce en dos tramos naturales y tres regulados. Los datos fueron analizados con estadística robusta y multivariante. Para cumplir el objetivo C, el muestreo georreferenciado anterior (junto con un muestreo dendrocronológico) fue acoplado con un modelo hidráulico en los dos tramos naturales, con el fin de obtener la serie temporal de cotas del agua a la que habían estado expuestas cada una de las plantas durante su vida. La respuesta de las especies y su posible agregación en gremios hidrológicos fue comparada mediante estadística robusta en relación a la duración de la inundación, duración de la inundación durante el período de crecimiento, duración continua de la inundación, frecuencia de inundación y elevación de la planta respecto al caudal base. Para lograr el objetivo D, toda la sección regulada del río Serpis (desde la presa Beniarrés - hasta la desembocadura; 40 km) se dividió en segmentos. En cada uno de ellos se llevó a cabo un inventario de flora y se aplicaron índices hidromorfológicos. Los segmentos fueron agrupados de acuerdo a su composición florística y características del hábitat fluvial utilizando diferentes técnicas de estadística multivariante. Finalmente se discutieron los factores principales que controlan los patrones espaciales de la composición florística, las características del hábitat fluvial, la calidad del hábitat ribereño y la heterogeneidad del hábitat fluvial. Para alcanzar el objetivo E, se combinaron series históricas de caudales y su manipulación humana con imágenes aéreas históricas (reveladoras de cambios en la cubierta vegetal) y observaciones de campo de la distribución de edades de la vegetación y morfología del corredor ripario del río Mijares en los últimos 60 años. De esta información, se extrajeron métricas del paisaje e índices hidrológicos para identificar y resumir los cambios espacio-temporales en la estructura de la ribera y en el régimen de caudales. El estudio a escala transversal nos ayudó a comprender en cada uno de los tramos la zonación de las especies leñosas riparias en función de la morfología y características del suelo, revelando que la alteración del caudal puede influir en la modificación de los patrones posicionales de las especies. Se definieron tres gremios hidrológicos: ¿altamente tolerante a la inundación¿, ¿tolerante intermedio¿ y ¿de transición entre ripario y terrestre¿. El estudio a escala longitudinal sugirió que la respuesta de la vegetación a un cambio hidrológico es altamente dependiente de la geomorfología local. Se constató que los factores principales que determinan los patrones hidromorfológicos y florísticos son un régimen de caudales artificial y altamente variable (identificado en los segmentos con peor calidad del hábitat ripario y fluvial), la presencia de estructuras laterales en el cauce y las características geomorfológicas. Como efectos importantes de la regulación del caudal a largo plazo, el estudio a escala espacio-temporal reveló un aumento en la cobertura y densidad de la vegetación leñosa, una deriva en la composición de especies y una disminución en las áreas de sedimento desnudo (esencial para el reclutamiento de las especies riparias pioneras), junto con una reducción sincrónica en la complejidad de la ribera. Estos cambios estarían relacionados con la disminución de la magnitud y variabilidad de los caudales en las últimas seis décadas. Solo una mejor comprensión de los procesos ecohidrológicos y de las implicaciones de la alteración hidrológica sobre los ecosistemas riparios mediterráneos podrá apoyar la integración eficaz de estos sistemas en las decisiones de gestión del agua. / Garófano Gómez, V. (2013). Riparian vegetation patterns according to hydrogeomorphological factors at different spatial and temporal scales in Mediterranean rivers [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/29395 / TESIS

Förändring av markanvändning i avrinningsområdet påverkar vattendrags karaktärer som vattenkvalitet och biodiversitet. Inom EU och Sverige utvecklas åtgärdsprogram för att förbättra ekologisk status av påverkade vattendrag. Det finns fortfarande osäkerheter när det kommer till effekten som jord- och skogsbruk har på bottenfauna. I studien användes data från ett nationellt miljöövervakningsprogram. Med dessa testades om proportionerna mellan jord- och skogsbruk påverkade på bottenfaunans diversitet och ekologisk status. 25 vattendrag i södra Sverige valdes med över 60% av någon av marktyperna inom avrinningsområdet inom tidserien 2007–2011. Statistiska analyserna gjordes i tre steg där det linjära sambandet mellan variabler testades. I första steget testades andelen jordbruksmark mot bottenfaunans diversitet och ekologisk status. I ett andra steg testades vattenkemi och vattenföring mot bottenfaunans diversitet och ekologisk status. I det sista steget testades andelen jordbruksmark mot vattenkemi och vattenföring. Resultaten visade att andelen jordbruksmark inte hade en effekt på bottenfaunans diversitet och ekologisk status, men hade en effekt på artsammansättningen. Specifikt, andelen jordbruksmark hade en effekt på vattendrags MISA-index, som indikerar vattnets surhetstillstånd. Ju mer jordbruksmark det fanns inom ett avrinningsområde desto mer surhetskänsliga arter hittades i vattendraget. Resultat visade därför att artsammansättningen skiljs mellan skogs- och jordbruksmark men inte antalet arter. För bättre utveckling av åtgärdsprogram behövs strandzons vegetationens betydelse undersökas mer. / Changes in catchment land-use affect stream morphology, water quality and biodiversity. In EU and Sweden measures are taken to improve the ecological status of streams. There are uncertainties about how land-use in terms of agriculture and forestry affect benthic invertebrates. In this study, data from national environmental monitoring programs was used. To test how the proportion of agricultural land-use in the catchment influence benthic invertebrate diversity and ecological status. In total 25 streams in southern Sweden with more than 60% of either land-use type in the catchment and data from 2007-2011 were selected. Statistical analyses were performed with simple linear regression in three steps. In a first step, the proportion of agriculture was tested against benthic invertebrate diversity and ecological status. In a second step, water chemistry and waterflow were tested against benthic invertebrate diversity and ecological status. In the final step, the proportion of agriculture was tested against water chemistry and waterflow. The results showed that the proportion of agricultural land-use did not influence benthic invertebrate diversity or ecological status. However, it influenced MISA, an index of stream acidification. The higher proportion of agricultural land-use there where in the catchment the more acid sensitive taxa where found. This can be explained by the fact that species composition alters between agriculture- and forestry land-use but not number of species or Simpson diversity-index. For better development of action programs, the importance of the riparian vegetation should be further investigated.

Human related activities have influenced the rivers of the southern Western Cape since as early as the 1700’s. As there is no detailed information available on ecological status of the Holsloot River, a tributary of the Breede River, this study aimed to gain insight into the effect of impacts associated with human activities on the habitat integrity of this river. The study intended to understand how seasonal changes, catchment characteristics and events are reflected in the ecological status of habitats along the river by applying bio-monitoring and river health measurements at selected sites in the upper, middle and lower reaches of the Holsloot River and compare the results to that of an undisturbed reference site. Results obtained in this study are compared with data gathered in 2008/2009 to determine if the ecological status of the river had changed in the period between the two sampling times. The study included assessment of the ecological status of the river based on standard bio-monitoring protocol (SASS5, IHI, IHAS and VEGRAI) as well as in situ water quality analysis (pH, dissolved oxygen, electrical conductivity and total dissolved solids). The construction of the instream Stettynskloof Dam changed the configuration of the riparian zone and river channel in the upper catchment area. Agricultural- and other human related activities, with consequent water abstraction, non-point-source pollution, loss of riparian vegetation, as well as dense stands of alien invader plants influence flow patterns and affects river ecology, especially in the dry summer months. Providing sufficient stream flow and adequate water levels, human related activities can create a larger variety of habitat types available that can support larger biodiversity and higher productivity. The level of inundation and stream flow, influenced by water abstraction as well as irrigation return-flow from extensive drainage systems especially in the dry months, contribute to the loss of biodiversity in the middle and lower reaches of the river. Where the upper reaches of the river are largely natural with few modifications, the habitat integrity deteriorates in the middle reaches so much so that ecosystem functioning are collectively impaired in lower reaches due to human related impacts. Sensitive macro-invertebrates found at lower seriously impacted parts of the river however, were in all probability washed down from lower impacted upstream habitats and may expectedly be able to again occupy habitats downstream if water quality and habitat availability improves. / Environmental Sciences / M. Sc. (Environmental Science)

Ph.d.-afhandling. Den kongelige Veterinær- og Landbohøjskole, 2002. / Haves også i trykt udg.

Το αντικείμενο της παρούσας διδακτορικής διατριβής είναι η οικολογική αξιολόγηση δύο ποτάμιων οικοσυστημάτων του Αχέροντα και του Λούρου, με βάση τις κοινότητες υδρόβιων μακροφύτων, την ποιότητα του νερού και τα υδρογεωμορφολογικά χαρακτηριστικά, καθώς και η ανάπτυξη και η οριστικοποίηση μεθοδολογίας για τη συλλογή, την επεξεργασία, την ανάλυση και την αξιολόγηση των δεδομένων πεδίου προσαρμοσμένης και εφαρμόσιμης στις ελληνικές συνθήκες. Οι επιμέρους στόχοι της διδακτορικής διατριβής διακρίνονται σε 4 επί μέρους κεφάλαια. Το πρώτο κεφάλαιο (Κεφάλαιο Α) αφορά τη διερεύνηση των αβιοτικών παραμέτρων που επηρεάζουν την κατανομή των υδρόβιων μακροφύτων κατά μήκος των δύο λεκανών απορροής. Στο δεύτερο κεφάλαιο (Κεφάλαιο Β) πραγματοποιείται διερεύνηση της δομής της παρόχθιας ζώνης και εκτίμηση των βασικών προτύπων οικολογικής διαβάθμισης και ανθρώπινης παρέμβασης. Στο τρίτο κεφάλαιο (Κεφάλαιο Γ) πραγματοποιείται αβιοτική τυπολογική διάκριση των θέσεων δειγματοληψίας με σκοπό τη μείωση της χωρικής διακύμανσης, έλεγχο της διαχρονικής και εποχικής διακύμανσης στην κατανομή των υδρόβιων μακροφύτων και καθορισμός των κοινοτήτων αναφοράς σε κάθε τμήμα του ποτάμιου οικοσυστήματος. Τέλος στο τέταρτο κεφάλαιο (Κεφάλαιο Δ) δίνεται η μεθοδολογία ανάπτυξης του συστήματος αξιολόγησης των επιπτώσεων των υδρομορφολογικών τροποποιήσεων στη σύνθεση και αφθονία των υδρόβιων και των παρόχθιων ειδών και η δημιουργία του Πολυμετρικού Δείκτη Μακροφύτων. Τα δεδομένα συλλέχθηκαν από συνολικά 32 θέσεις δειγματοληψίας οι οποίες επιλέχθηκαν με κριτήρια 1) την κάλυψη ευρείας διαβάθμισης χρήσεων γης, 2) την επιλογή περισσοτέρων του ενός τμημάτων με παρόμοια γεωμορφολογικά χαρακτηριστικά, 3) τη σταθερή απόσταση μεταξύ των θέσεων δειγματοληψίας, 4) την ομοιογένεια των τμημάτων και τέλος 5) την προσβασιμότητα στα σημεία δειγματοληψίας καθ’ όλη τη διάρκεια της παρακολούθησης. Με βάση τα πιο πάνω κριτήρια επιλέχθηκαν 15 θέσεις δειγματοληψίας στη λεκάνη απορροής του ποταμού Αχέροντα [11 Αχέροντας (A1-8 & A11-13), 1 Κωκυτός (A9), 1 Βουβό Ρέμα (A15), 2 αρδευτικά κανάλια (A10 & A14)] και 17 Θέσεις Δειγματοληψίας στη λεκάνη απορροής του ποταμού Λούρου (S1-17). Η περιοχή αξιολόγησης περιλαμβάνει ένα ομοιογενές τμήμα ποταμού μήκους 100 m, το οποίο χωρίστηκε σε τρία επί μέρους τμήματα: α) το τμήμα της κοίτης το οποίο καλύπτεται με νερό, β) τα κράσπεδα, δηλαδή το ενεργό τμήμα της κοίτης γ) την παρόχθια-δασική ζώνη. Για τη συλλογή και την αξιολόγηση της υδρόβιας και παρόχθιας βλάστησης πραγματοποιήθηκαν εποχικές δειγματοληψίες κατά τη διάρκεια των βλαστητικών περιόδων των ετών 2005-2007 και η αφθονία ειδών καταγράφηκε με την 5-βάθμια κλίμακα DAFOR (5: κυριαρχία και 1: απουσία). Στο πεδίο μετρήθηκαν επίσης οι φυσικοχημικές παράμετροι του νερού (pH, διαλυμένο οξυγόνο, θερμοκρασία, αγωγιμότητα, ταχύτητα νερού και μέσο βάθος νερού) και συλλέχθηκαν δείγματα επιφανειακού νερού για χημικές αναλύσεις θρεπτικών αλάτων αζώτου (ΝΟ3-Ν, ΝΟ2-Ν, ΝΗ4-Ν) και φωσφόρου (PO4-P & TP), Chl-a και αλκαλικότητας. Επίσης καταγράφηκαν στο πεδίο και υπολογίστηκαν στο εργαστήριο υδρογεωμορφολογικά στοιχεία και δεδομένα τοπίου τα οποία αφορούν τόσο την περιοχή αξιολόγησης όσο και όλη τη λεκάνη απορροής. Για τη στατιστική επεξεργασία των δεδομένων χρησιμοποιήθηκαν 1) Ιεραρχική Ανάλυση Συστάδων για βιοτική τυπολογία των υδρόβιων μακροφύτων κατά μήκος των ποταμών και για τον προσδιορισμό της δομής της παρόχθιας βλάστησης, 2) Ανάλυση Ειδών Δεικτών (ISA), για την περιγραφή της ιεραρχικής δομής της κάθε ομάδας και για τη διάκριση των σημαντικών Ειδών-Δεικτών (IndSps), και 3) Ταξιθέτηση όπου χρησιμοποιήθηκαν τόσο έμμεσες όσο και άμεσες αναλύσεις διαβάθμισης. Στις έμμεσες αναλύσεις διαβάθμισης χρησιμοποιήθηκαν α) Ανάλυση Κύριων Συνιστωσών (PCA), για τον προσδιορισμό των Γεωμορφολογικών Ενοτήτων και στον Καθορισμό της κλίμακας πιέσεων και β) Βελτιωμένη Ανάλυση Αντιστοιχιών (DCA), για τη χωρική κατανομή της αφθονίας των ειδών και τον έλεγχος των εποχικών και διαχρονικών διακυμάνσεων των κοινοτήτων αναφοράς. Ως άμεσες αναλύσεις διαβάθμισης χρησιμοποιήθηκαν α) Ανάλυση Πλεονασμού (RDA), για συσχέτιση των φυσικοχημικών χαρακτηριστικών του νερού με τα είδη δείκτες και β) Ανάλυση Κανονικών Αντιστοιχιών (CCA), για συσχέτιση των γεωμορφολογικών παραμέτρων με τα είδη-δείκτες και τον καθορισμό κύριων μεταβλητών υποβάθμισης της παρόχθιας ζώνης. Τα αποτελέσματα έδειξαν ότι κατά μήκος των δύο λεκανών απορροής διακρίνονται έξι Ομάδες Βλάστησης με παρόμοιο περιβαλλοντικό πρότυπο (πηγές-εκβολές). Στις ορεινές περιοχές και των δύο λεκανών απορροής (Αχέροντα και Λούρου) κυριαρχούν τα είδη Pteridium aquilinum, είδη βρυόφυτων και είδη του γένους Carex, C. acuta για τον ποταμό Αχέροντα και C. pendula για τον ποταμό Λούρο. Στο μέσο ρου των ποταμών διακρίθηκαν 4 διαφορετικές ομάδες βλάστησης. Ο διαφορετικός βαθμός ανθρωπογενούς τροποποίησης των εκβολών στις δύο λεκάνες απορροής αντανακλά και στη σύνθεση των φυτοκοινοτήτων όπου στις εκβολές του ποταμού Αχέροντα κυριαρχούν χλωροφύκη και το ανθεκτικό είδος Potamogeton pectinatus ενώ στον ποταμό Λούρο οι εκβολές χαρακτηρίζονται από την απουσία χλωροφυκών και την κυριαρχία του είδους Potamogeton nodosus. Οι σημαντικότεροι φυσικοχημικοί παράγοντες οι οποίοι επηρεάζουν την κατανομή των υδρόβιων μακροφύτων κατά μήκος των λεκανών απορροής της περιοχής μελέτης είναι: το pH, η αγωγιμότητα, η θερμοκρασία νερού, η μέση ταχύτητα νερού, οι συγκεντρώσεις των αλάτων αζώτου (ΝΟ3-Ν, ΝΗ4-Ν, ΝΟ2-Ν) και ορθοφωσφορικών (PO4-P) και Chl-a. Ενώ οι σημαντικότεροι γεωμορφολογικοί παράγοντες είναι: το υψόμετρο, το υπόστρωμα κοίτης, το πλάτος της κοίτης, η σκίαση της κοίτης, ο τύπος ενδιαιτήματος και το βάθος του νερού. Η διερεύνηση της δομής της παρόχθιας ζώνης έδειξε την ύπαρξη 4 διαφορετικών ομάδων παρόχθιας βλάστησης. Οι ορεινές περιοχές και άνω μέσος ρους και των δύο λεκανών απορροής χαρακτηρίζεται από την κυριαρχία των ειδών Platanus orientalis και Quercus coccifera. Οι βασικές τροποποιήσεις που δέχονται οι περιοχές αυτές αφορούν μεταβολές σε τοπικό επίπεδο γεγονός που επιβεβαιώνεται από τις υψηλές τιμές δείκτη QBR. Οι πεδινές περιοχές χαρακτηρίζονται από την κυριαρχία των ειδών Salix alba και Populus alba. Οι σημαντικότερες πιέσεις που δέχεται το πεδινό τμήμα των ποταμών αφορούν τις γεωργικές δραστηριότητες, τη δημιουργία τεχνικών αναχωμάτων, την ενίσχυση της όχθης, τις αμμοχαλικοληψίες, τη διευθέτηση και την ευθυγράμμιση της όχθης. Το είδος Phragmites australis χαρακτηρίζει τις εκβολές των ποταμών, ενώ σημαντική ήταν η αφθονία των ειδών Arundo donax και του εισαγόμενου είδους Eucalyptus camaldulensis. Σύμφωνα με τα αποτελέσματα η αφθονία των ειδών ήταν χαμηλή σε όλες τις Ομάδες Βλάστησης. Εφαρμόστηκαν επίσης οι δείκτες: QBR ο οποίος αποτελεί μέτρο αξιολόγησης της ποιότητας της παρόχθιας ζώνης, HQA όπου αποτελεί δείκτη αξιολόγησης της οικολογικής ποιότητας του ποτάμιου ενδιαιτήματος και HMS ο οποίος είναι δείκτης ανθρωπογενούς τροποποίησης του ενδιαιτήματος. Τα αποτελέσματα από την εφαρμογή των πιο πάνω δεικτών έδειξαν ότι η οικολογική ακεραιότητα παρόχθιας ζώνης αυξάνει τη συνολική οικολογική ποιότητα. Σύμφωνα με τις υδρογεωμορφολογικές μεταβλητές, οι οποίες εξετάστηκαν, οι θέσεις δειγματοληψίας ομαδοποιηθήκαν σε τρεις Γεωμορφολογικές Ενότητες οι οποίες αντιστοιχούν στο Ορεινό, Πεδινό και Εκβολικό Τμήμα των ποταμών. Σε κάθε Γεωμορφολογική Ενότητα πραγματοποιήθηκε ανάλυση πιέσεων και διακρίθηκαν οι σταθμοί αναφοράς οι οποίοι για το ορεινό τμήμα ήταν 7, στο πεδινό 3 ενώ στο εκβολικό τμήμα μόνο 2 σταθμοί χαρακτηρίστηκαν ως περιοχές αναφοράς. Σε κάθε Γεωμορφολογική Ενότητα διερευνήθηκαν οι εποχικές και διαχρονικές μεταβολές στη σύνθεση των κοινοτήτων αναφοράς και προσδιορίστηκε δομή τους. Τα αποτελέσματα έδειξαν, ότι η σύνθεση των κοινοτήτων αναφοράς στο πεδινό τμήμα των ποταμών φαίνεται να παραμένουν σταθερές στις φυσικές διακυμάνσεις. Από την άλλη, οι μικρές διαφορές οι οποίες καταγράφηκαν στο ορεινό και εκβολικό τμήμα των ποταμών οφείλονται κυρίως στην όψιμη Άνοιξη και στις υψηλές θερμοκρασίες που επικρατούν κατά τους καλοκαιρινούς μήνες και εντοπίζονται στο μειωμένο αριθμό των βρυοφύτων και πτεριδοφύτων αντίστοιχα. Τέλος σύμφωνα τα αποτελέσματα η περίοδος διεξαγωγής των δειγματοληψιών προσδιορίζεται ανάμεσα στο χρονικό διάστημα μεταξύ τέλος Απριλίου και τέλος Σεπτεμβρίου αφού σε αυτό το χρονικό διάστημα οποιαδήποτε επίσκεψη στο πεδίο αναμένεται να δώσει την ίδια σύνθεση των κοινοτήτων αναφοράς. Διερευνήθηκαν, στο μεγαλύτερο δυνατό βαθμό, όλες οι πιθανές παράμετροι και πιέσεις, οι οποίες επηρεάζουν τη σύνθεση και την κατανομή υδρόβιων μακροφύτων κατά μήκος των λεκανών απορροής. Οι σημαντικότερες πιέσεις που δέχεται η περιοχή μελέτης επηρεάζουν κυρίως τα υδρομορφολογικά χαρακτηριστικά του οικοσυστήματος. Ως πιο αξιόπιστος και ολιστικός τρόπος αξιολόγησης της οικολογικής κατάστασης της περιοχής μελέτης επιλέχθηκε η Πολυμετρική προσέγγιση. Σαν πίεση επιλέχθηκε η υδρομορφολογική τροποποίηση. Συνολικά, διερευνήθηκαν 86 υποψήφιες μετρικές οι οποίες ανήκουν σε 5 κατηγορίες: 1) Αφθονίας/Ποικιλότητας, 2) Τροφικής κατάστασης, 3) Σύνθεσης φυτικών κοινοτήτων, 4) Ποιότητας Δομής της παρόχθιας ζώνης και 5) Ευαισθησίας/Ανοχής, οι οποίες προέρχονται από τις βιοκοινότητες του υγρού δίαυλου, της ενεργής κοίτης και της όχθης. Ο καθορισμός της κλίμακας πιέσεων πραγματοποιήθηκε με τη χρήση της Ανάλυσης Κύριων Συνιστωσών (PCA) με 36 υδρομορφολογικές μεταβλητές υποβάθμισης σε 2 χωρικά επίπεδα: Μακροκλίμακας και Μικροκλίμακας. Στον τελικό Πολυμετρικό δείκτη συμπεριλήφθηκαν μόνο οι μετρικές οι οποίες ικανοποιούσαν συγκεκριμένα κριτήρια. Τα κριτήρια αυτά αφορούσαν: την εκπλήρωση του κριτηρίου από την εφαρμογή των θηκογραμμάτων με απολήξεις (δηλαδή εξαιρούνται οι μετρικές οι οποίες έχουν στενό εύρος τιμών, πολλές ακραίες τιμές και πολλές απόμακρες παρατηρήσεις), την αποφυγή αλληλεπικαλυπτόμενων πληροφοριών (Spearman ǀrǀ>0,800) και την υψηλή συσχέτιση με τις κλίμακες πιέσεων (Spearman ǀrǀ>0,500). Συνολικά από τις 86 υποψήφιες μετρικές οι 6 θεωρήθηκαν ως κεντρικές μετρικές αφού ικανοποίησαν όλα τα πιο πάνω κριτήρια. Οι κεντρικές μετρικές είναι: 1) Δείκτης IBMR, 2) Δείκτης QBR, 3) Αριθμός Βρυοφυτικών taxa, 4) % Είδη Αναφοράς, 5) % Νιτρόφιλα είδη και 6) % Ελοφυτικά είδη (Phe_herbids). Ο Πολυμετρικός Δείκτης Μακροφύτων (Multimetric Macrophyte Index-MMI) είναι ο αριθμητικός μέσος των 6 κανονικοποιημένων «κεντρικών» μετρικών. Κανονικοποίηση καλείται η προσαρμογή του εύρους τιμών της κάθε μετρικής στο ενδεικτικό όριο από 0 μέχρι 1. Σημαντικό σημείο στην κανονικοποίηση των μετρικών είναι ο καθορισμός του ανώτερου (upper anchor) και κατώτερου ορίου (lower anchor) της κάθε «κεντρικής» μετρικής. Το ανώτερο και το κατώτερο όριο δείχνουν το ενδεικτικό εύρος της κάθε μετρικής όπου το ανώτερο όριο (anchor) αντιστοιχεί στην τιμή της μετρικής κάτω από τις συνθήκες αναφοράς. Στην παρούσα εργασία για το ανώτερο όριο κάθε μετρικής επιλέχθηκε το 75ο εκατοστημόριο (percentile) των μη διαταραγμένων περιοχών και για το κατώτερο όριο το 5ο εκατοστημόριο (percentile) των διαταραγμένων περιοχών. Για την αξιολόγηση της οικολογικής ποιότητας, των θέσεων δειγματοληψίας, με βάση τον Πολυμετρικό Δείκτη Μακροφύτων, απαιτείται ο καθορισμός των ορίων μεταξύ των 5 ποιοτικών κλάσεων όπως απαιτεί η Οδηγία Πλαίσιο για τα Ύδατα 2000/60/ΕΕ. Τα όρια του δείκτη καθορίστηκαν υπολογίζοντας αρχικά το όριο μεταξύ της «Υψηλής»/«Καλής» οικολογικής κατάστασης το οποίο ορίστηκε ως το 25o εκατοστημόριο μη διαταραγμένων περιοχών (0,623). Το υπόλοιπο εύρος τιμών χωρίστηκε σε τέσσερις ίσες κλάσεις. Τα αποτελέσματα έδειξαν, ότι ο ΜΜΙ δίνει πολύ καλή ένδειξη της οικολογικής κατάστασης των μεσαίου μεγέθους πεδινών ποταμών της Δυτικής Ελλάδας (RM-2). Σύμφωνα με τα αποτελέσματα το 29,6% των θέσεων δειγματοληψίας (8 θέσεις) ταξινομήθηκαν στην Υψηλή οικολογική κατάσταση, το 4% (14 θέσεις) στην Καλή οικολογική κατάσταση, το 22,3% (6 θέσεις) στην Μέτρια, το 29,6% (8 θέσεις) στην Φτωχή και μόνο το 3,7% (1 θέση) χαρακτηρίστηκε ως Κακής Οικολογικής Ποιότητας. Η παρούσα Διδακτορική Διατριβή, αποτελεί µια πρώτη προσπάθεια στον Ελληνικό χώρο για δημιουργία ενός ολοκληρωμένου συστήματος παρακολούθησης της οικολογικής ποιότητας των ρεόντων υδάτων με χρήση των υδρόβιων μακροφύτων ως βιολογικών ποιοτικών στοιχείων. Η μεθοδολογία αναπτύχθηκε και εφαρμόστηκε πιλοτικά σε δύο λεκάνες απορροής στα μεσαίου μεγέθους πεδινά ποτάμια της Ηπείρου Αχέροντα και Λούρου ακολουθώντας πιστά τις απαιτήσεις και τις κατευθυντήριες γραμμές της Ευρωπαϊκής Οδηγίας Πλαίσιο για τα Ύδατα 2000/60/ΕΕ. Επίσης το σύστημα αξιολόγησης το οποίο προτείνεται αναπτύχθηκε με σκοπό την εφαρμογή του σε όλα τα παρόμοιου τύπου ποτάμια της Ελλάδας αφού τόσο ο κατάλογος με τις υποψήφιες μετρικές όσο και ο ίδιος ο πολυμετρικός δείκτης μπορεί να ελεγχθεί και σε άλλους τύπους ποταμών της Ελλάδας (RM-1, RM-3, RM-4 & RM-5). Τέλος, τα αποτελέσματα που προκύπτουν συμβάλλουν στην ορθολογικότερη διαχείριση και ανάδειξη των ποτάμιων ενδιαιτημάτων και στην εφαρμογή της περιβαλλοντικής πολιτικής του νερού στην Ελλάδα. / The main objective of the present thesis was the ecological assessment of two river basins, Acheron and Louros; based on aquatic plant communities, water quality and hydromorphological characteristics, as well as, the development of a methodology for collecting, analyzing and assessing field data, specific adapted to Greek conditions. The specific objectives of the doctoral thesis include: a) the investigation of abiotic parameters influencing the distribution of aquatic macrophytes along the two river basins (Chapter A); b) the investigation of riparian zone structure and the assessment of “key” environmental gradient and human intervention (Chapter B); c) abiotic typology for reducing the spatial variability, monitoring the temporal and seasonal variation in of aquatic macrophytes distribution, and determination of reference communities in each geomorphological unit of the riverine ecosystem (Chapter C), and d) the development of the Macrophyte Multimetric Index (MMI) which is described step by step in Chapter D. Data were collected from 32 sampling sites, which were chosen according to the following criteria: i) covering a wide gradient of land used i.e. from natural to artificial; ii) selection of more than one site with similar geomorphological characteristics; iii) the constant distance between sampling sites; iv) the homogeneity of the sampling sites, and v) the accessibility to the sampling points throughout the duration of monitoring. Based on the above criteria, 15 sites were selected along Acheron river basin [11, Acheron river (A1-8 & A11-13); 1, Cocytos (A9); 1, Vouvo Rema (A15); 2, irrigation canals (A10 & A14)]. Additionally, 17 sampling sites were selected along Louros river (S1-S15). The survey area was subdivided into 3 zones, a) the wetted part of the channel, b) the marginal-active channel and c) the riparian woodland plot. The survey area length was standardized at 100 m according to the widely accepted methods [e.g. Mean Trophic Rank, (MTR); Riparian Forest Quality, (QBR)]. On the other hand, the width of each plot includes the area between, the end of the active channel and the riparian woodland. The field surveys were conducted during the vegetation periods (April to September) of the years 2005 to 2007. The coverage of each macrophyte species was visually estimated using DAFOR 5-point scale (1: Rare, 2: Occasional, 3: Frequent, 4: Abundant and 5: Dominant). Also, water physicochemical parameters such as temperature, DO, pH, conductivity, were measured in situ using portable equipment (WTW340i/SET). Additionally, surface water samples were collected for determination of nutrients such as nitrate and nitrite nitrogen, total nitrogen, ammonium, phosphorus nutrients (soluble reactive phosphorus and total phosphorus), Chl-a, and alkalinity (HCO-3, CO3=). Nutrient samples were collected and analyzed according to APHA standard analytical method (APHA, 1989). For the statistical processing of the data were used: 1) Hierarchical cluster analysis (Bray-Curtis), for the biotic typology of macrophyte data along river basins and to determine the structure of the riparian vegetation; 2) Indicator Species Analysis, was used to describe the hierarchical structure of each group to distinguish the indicator species (Ind_Sps) for each vegetation group; 3) Ordination, which were used both Indirect and Direct gradient analyses. Indirect gradient analyzes were used: a) Principal Component Analysis (PCA), to identify Geomorphological Units and to determining the pressure gradients; and b) Detrended Correspondence Analysis (DCA), for researching the spatial distribution of species abundance, and for seasonal and inter-annual variability of the reference communities. For direct gradient analyzes were used: a) Redundancy Analysis (RDA), for correlation between, water physicochemical parameters and Indicator species; and b) Canonical Correspondence Analysis (CCA), for correlation between geomorphological characteristics and indicator species, as well as, to determine the key variables best explain the degradation of the riparian zone. The results showed that along the two river basins, six vegetation groups can be distinguished, with similar environmental gradient pattern (springs-estuaries). The dominant species for the upper part of Acheron and Louros river basins were: Pteridium aquilinum, bryophyte taxa and Carex species (C. acuta and C. pendula respectively). Moreover, four different vegetation groups were characterized the middle part of both river basins. The different degree of anthropogenic alteration of river estuaries (Acheron and Louros), reflects in the composition of the plant communities. The estuaries of Acheron were characterized by the dominant of green algae and the tolerant species Potamogeton pectinatus, while the estuaries of Louros river was characterized by the absence of green algae and the dominance of Potamogeton nodosus. The most important physicochemical variables that significantly affect the distribution of aquatic macrophytes along the river basins were: pH, conductivity, water temperature, and mean water velocity, concentrations of nitrogen nutrients (ΝΟ3-Ν, ΝΗ4-Ν, ΝΟ2-Ν) and orthophosphate (PO4-P), as well as, Chl-a. Whereas, the most important geomorphological factors were: altitude, bed substrate, channel width, channel shading, habitat type, water depth. Hierarchical cluster analysis distinguished five vegetation groups of the riparian zone of the study area. The following groups were indentified and characterized by the dominant riparian species. The upper reaches were characterized by the Vegetation Group Ia: Platanus orientalis and Ib: Quercus coccifera-P. orientalis; the middle reaches by II: Salix alba and Populus alba; and lowland areas until estuaries by III: Phragmites australis. Vegetation Group IV was characterized by the dominant of non native species Eucalyptus camaldulensis and the giant reed Arundo donax. According to the results, species abundance was low in all Vegetation Groups. Also, the applied indices Riparian quality assessment (QBR), Habitat Quality Assessment (HQA) and Habitat Modification Score (HMS) revealed that the ecological integrity of the riparian zone increases the overall ecological quality of the riparian ecosystem. According to the hydromorphological characteristics, the sampling sites were grouped into three Geomorphological Units: Upper –Middle reaches-Estuaries. In each Geomorphological Unit (GU) a pressure gradient analysis was performed, and the reference sites were identified. For GU I the number of reference sites was 7, for GU II 3 reference sites were identified, while, for the estuaries the number of the reference sites were only 2. Also in each GU the seasonal and inter annual changes in reference communities, were investigated, and the structure of the reference community was determined. The results showed that the composition of the reference communities in lowland rivers appears to remain constant in the physical fluctuations. On the other hand, the slight differences which were recorded, in the GU I, during spring and summer sampling, referred to the reduction of bryophytes. This reduction might be cause due to the late spring (May), at the upper part of Louros river. Also, differences were recorded between autumn and spring samplings, in the GU III (estuaries). Those differences referred to the reduction of pteridophytes, maybe due to the high temperatures that prevail during the summer months. Finally, the sampling period could be set between late April and late September, since, during this period, any field visit will give the same composition of the reference communities. We investigated, to the greatest possible degree, all the potential parameters and pressures, which could influence the composition and distribution of aquatic macrophytes along the river basins. The most significant effect, from the current pressures, in the studied area, is the alteration of the hydromorphological characteristics of the ecosystem. The Multimetric approach was chosen, as the most holistic and reliable evaluation method of the ecological status of the study area. A preliminary list of potential plant metrics was compiled from a review of the literature, and in situ observations of plant community patterns. The metrics were grouped into five categories, so that each one representing a different ecological aspect of aquatic and riparian plant communities: 1) Richness/Diversity, 2) Trophic status, 3) Composition, 4) Riparian Integrity, and 5) Sensitivity/Tolerance. A total of 86 metrics were tested, of which 7 belonged to the category of "Abundance/Diversity", 4 to the category of "Trophic” status, 46 metric referred to “Composition” of plant communities, 11 referred to "Riparian Integrity”, and finally, 18 belonged to “Sensitivity/Tolerance” category. The estimation of the pressure gradient was performed using Principal Component Analyses (PCAs), with 36 hydromorphological degradation variables in 2 spatial scales: macroscale and microscale. Candidate metrics, which can be identified as robust and most informative, are scrutinized further, in the process of selecting core metrics. To be selected as a core metric the following aspects have to be considered: 1) core metrics should cover different metric types (see above); 2) metrics should not give redundant information. Inter-correlation tests between candidate metrics were carried out to detect redundant metrics (threshold value Spearman’s r<0.800). In case of redundancy we further investigate: the correlation of each metric with stress gradient (threshold value Spearman’s r<0.05); the correlation of each of the pair of metrics with the other metrics in order to finally omit the one that showed the higher overall mean correlation; and how well they separate stressed from unstressed sampling sites (graphical analysis of box-whisker plots). The final index includes 6 metrics out of 86 candidated metrics. The core metrics are: 1) IBMR, 2) QBR, 3) Number of Bryophyte, 4) % Reference Species, 5) % Nitrophilous taxa and 6) % Helophyte taxa (Phe_herbids). The different numerical scales of each core metric (e.g. abundance class, number of individuals) were normalized to unitless scores between 0 and 1. The upper and lower anchors mark the indicative range of a metric. The upper anchor corresponds to the upper limit of the metric’s value under reference conditions, and it was set as the 75th of the unstressed sites. The lower anchor corresponds to the lower limit of the metric’s value under the worst attainable conditions and it was set as 5th percentile of stressed sites. The multimetric index was calculated as the arithmetic mean of the normalized metrics (Böhmer et al., 2004). The final Multimetric Index provides a score that represents the overall relationship between the combined values of the biological parameters observed for a given site and the expected value under reference conditions. This score is – as for single metrics – expressed as a numerical value between zero and one. This range can be subdivided into any number of categories corresponding to various levels of impairment. To determine the boundaries of Multimetric Index, the 25th percentile of the unstressed sites (0,623) was set as the boundary for the high/good ecological class. We propose quality classes with equal ranges, to provide five ordinal rating categories for assessment of impairment in accordance with the demands of the WFD. The application of Macrophyte Multimetric Index (MMI) in the medium lowland rivers of Greece led to the ecological classification in five quality classes. The results indicated that the 29.6% of the sampling sites (8 sites) were classified in High ecological status, 4% (4 sites) in Good ecological status; the 22.3% (6 sites) in Moderate, 29.6% (8 sites) in the Poor and only 3.7% (1 site) was characterized as Bad ecological quality. The current doctoral thesis was the first attempt in the Greece, for establishing an integrated monitoring system of ecological quality of running water ecosystems, using aquatic macrophytes as biological quality element. The methodology developed, was pilot implemented in two river basins, Acheron and Louros, in Epirus, following the requirements and guidelines of the European Water Framework Directive, 2000/60/EE. Also, the evaluation system was developed to be proposed for implementation at all similar river types of Greece, since both the list of candidate metrics and the Multimetric Index itself, can be tested in other types of Greek rivers (RM-1, RM-3, RM-4 & RM-5). Finally, the results, will contribute to the sustainable management and conservation of riverine ecosystems, as well as, to the implementation of the water environmental policy in Greece.