Durch parallele SPOT-Synthese wurden trisamino- und amino-oxo-substituierte [1,3,5]-Triazine auf Zellulose- und Polypropylenmembranen dargestellt. Neben der Entwicklung geeigneter Linkerstrategien und dem Einsatz von Aminen und Phenolaten als Bausteine konnte eine Mikrowellenbestrahlung für die Substitution an den membrangebundenen Monochlor-[1,3,5]-triazinen nutzbar gemacht werden. Der Einsatz von gasförmiger TFA zur Abspaltung der Syntheseprodukte von den planaren Oberflächen erlaubte den Erhalt der örtlichen Adressierbarkeit der Verbindungen für Analyse und Screening. Zusätzlich wurde ein neues Konzept für die Synthese von makrocyclischen Peptidmimetika entwickelt. Diese Methode bedient sich der sequenziellen SNAr-Reaktion von ursprünglich orthogonal geschützten Aminogruppen eines Peptides und anderer linearer Oligomere an halogenierten Heteroaromaten wie 2,4,6-Trichloro-[1,3,5]-triazin, 2,4,6-Trichloropyrimidin, 4,6-Dichloro-5-nitropyrimidin und 2,6,8-Trichloro-7-methyl-7H-purin. Die Möglichkeiten dieses neuen Zugangs zu Makrocyclen wurde systematisch mittels SPOT-Synthese untersucht. So wurden Fragen wie zugängliche Ringgrössen, Kompatibilität mit Aminosäuren, Cyclisierungsrichtungen und Verwendung von unterschiedlichen linearen Oligomeren adressiert. Es stellte sich heraus, dass eine Reihe von Peptidmimetika mit unterschiedlichen Ringgrössen (11- bis 37-gliedrige Ringe) und verschiedenen chemischen Strukturen des Rückgrades erhalten werden können. Die erhaltenden [1,3,5]-Triazin-Bibliotheken wurden sowohl in einem Festphasen-Screening als auch in einem Assay in Lösung eingesetzt. Es gelang, de novo Bindungspartner für den monoklonalen Antikörper TAB-2 aus einer Bibliothek aus 8000-zellulosegebundenen [1,3,5]-Triazinen zu finden und neuartige cyclische Peptid-Triazinderivate als Agonisten für einen Somatostatinrezeptor zu entwickeln. / Effective spatially addressed parallel assembly of trisamino- and amino-oxy-1,3,5-triazines was achieved by applying the SPOT-synthesis technique on cellulose and polypropylene membranes. In addition to developing a suitable linker strategy and employing amines and phenolate ions as building blocks, a highly effective microwave assisted nucleophilic substitution procedure at membrane-bound monochlorotriazines was developed. The 1,3,5-triazines obtained could be cleaved in parallel from the solid support by TFA-vapor to give compounds adsorbed on the membrane surface in a conserved spatially addressed format for analysis and screening. A novel concept for the synthesis of macrocyclic peptidomimetics which incorporate heteroaromatic units was developed. The method involves sequential SNAr reactions of former orthogonally protected amino groups of peptides and other linear oligomers on halo-genated heterocycles such as 2,4,6-trichloro-[1,3,5]-triazine, 2,4,6-trichloropyrimidine, 4,6-dichloro-5-nitropyrimidine and 2,6,8-trichloro-7-methyl-7H-purine. The scope of this novel solid phase approach was systematically evaluated by means of the SPOT-synthesis metho-dology. Besides the question of the accessibility of different ring sizes and the compatibility with protecting groups of commonly used amino acids, cyclization direction and the applicability of the technique towards peptidomimetics was studied. It was found that the procedure is well suited to assemble a wide variety of cyclic peptidomimetics differing in both size (11 to 37-membered rings) and chemical nature of the assembled backbones. The obtained [1,3,5]-triazine libraries were subjected to heterogeneous and homogeneous screening assays. De novo binding partners for the monoclonal antibody Tab2 were detected from a 8000-membered library of cellulose-bound 1,3,5-trazines. In addition novel cyclic peptide-triazine derivatives were identified as agonists for a somatostatin receptor.

Ziel dieser Arbeit ist es eine Toolbox zur Charakterisierung der anomalen Diffusion von Tracerpartikeln in dicht gepackten Systemen mit Fluoreszenz-Korrelationsspektroskopie (FCS) zur Verfügung zu stellen. Es wird gezeigt, dass die robusten Informationen über die Wahrscheinlichkeitsdichtefunktion (PDF) der Verschiebung des Tracers im asymptotischen Verhalten der FCS-Kurven auf langen, sowie auf kurzen Zeitskalen enthalten sind. So liefert die Analyse des Kurzzeitverhaltens zuverlässige Aussagen über die Werte des Exponenten der anomalen Diffusion, des Diffusionskoeffizienten und der niedrigeren Momente der PDF. Dies erlaubt es eine Gaußverteilung zu bestätigen oder zu widerlegen. Der Test auf Gaußverteilung könnte als Index verwendet werden, um die richtige Form der PDF aus einer Reihe von konkurrierenden Ergebnissen zu erraten. Darüber hinaus untersuchen wir die Konsequenz der nicht skalierenden PDF auf Ergebnis der FCS-Kurven. Wir berechnen die FCS für ein Continuous Time Random Walk Modell mit Wartezeiten gemäß einer Lévy-stabilen Verteilung mit exponentiellem cut-off. Die Ergebnisse zeigen, dass obwohl die Abweichungen vom Gauß’schen Verhalten bei der asymptotischen Analyse erkannt werden können, ihre Körper immer an Formen für die normale Diffusion perfekt angepasst werden können. Schließlich schlagen wir einen alternativen Ansatz für die Durchführung von Spot Variation FCS mit dem gewöhnlichen FCS-Setup vor. Wir führen eine nicht-lineare Transformation ein, die auf das mit Binning oder Kernel smoothing method geglättete Intensitätsprofil der detektierten Fluoreszenzphotonen angewendet wird. Ihre Autokorrelation imitiert die FCS-Kurven für die Größen des Laserspots, die im Experiment effektiv kleiner als die anfängliche Größe sind. Die erhaltenen FCS-Kurven werden verwendet, um künstliche dicht gepackte Systeme sowie lebende Zellen auf Nano-Domänen oder Barrieren hin zu untersuchen. / The objective of this thesis is to provide a toolbox for characterization of anomalous diffusion of tracer particle in crowded systems using fluorescence correlation spectroscopy (FCS). We discuss that the robust information about the probability density function (PDF) of the particle’s displacement is contained in the asymptotic behaviour of the FCS curves at long and short times. Thus, analysis of the short-time behaviour provides reliable values of exponent of anomalous, diffusion coefficient and lower moments of the PDF. This allows one to to confirm or reject its Gaussian nature. The Gaussianity test could be then used to guess the correct form of the PDF from a set of competing models. We show the applicability of the proposed analysis protocol in artificially crowded systems and in living cell experiments. Furthermore, we investigate the consequence of non-scaling PDF on the possible results of the FCS data. As an example of such processes, we calculate the FCS curve for a continues time random walk model with waiting times delivered from Lévy-stable distribution with an exponential cut-off in equilibrium. The results indicate that, although the deviations from Gaussian behaviour may be detected when analyzing the short- and long-time asymptotic of the corresponding curves, their bodies are still perfectly fitted by the fit form used for normal diffusion. Finally, we propose an alternative approach for performing spot variation FCS using an ordinary FCS set-up. We introduce a non-linear transformation which applies on the smoothed intensity profile of the detected fluorescence photons with binning or smoothing kernel method. Autocorrelation of the generated intensity profiles mimic the FCS curves for the sizes of laser spots which are effectively smaller than the initial one in the experiment. The obtained FCS curves are used to investigate the presence of nano-domains or barriers in artificially crowded systems and in living cells.

A soldagem a ponto por resistência é o processo de união de partes mais comum utilizado na indústria automobilística. Vários métodos de representação de pontos de solda estão disponíveis, e este trabalho se propõe comparar dois métodos de modelagem de pontos de solda através de um estudo de correlação, em termos da força máxima suportada pela junta soldada, entre os resultados de testes virtuais e de laboratório. Estes métodos de representação dos pontos de solda são os elementos de viga e os elementos sólidos. Os testes utilizados neste estudo de correlação carregam o corpo de provas sob ação de forças de cisalhamento e normal aplicadas diretamente no ponto de solda. E para completar este estudo de correlação, o entendimento dos modos e critérios de falha de juntas soldadas também é necessário. Os modelos de elementos finitos dos corpos de prova gerados para serem testados sob condições de cisalhamento e de força normal são detalhados pela apresentação dos tipos de materiais, elementos, formulações de contato e carregamento utilizados. Estes modelos foram analisados pelo LS-DYNA, que é um código de elementos finitos de propósito geral para análise de respostas dinâmicas provenientes de grandes deformações de estruturas. Da mesma forma, os testes físicos e os corpos de prova utilizados são detalhados, e os resultados provenientes dos testes são comparados aos obtidos nas simulações. Dos resultados obtidos, a melhor correlação se deu com a utilização do elemento sólido para representar o ponto de solda, com diferenças entre os resultados físicos e virtuais em torno de 4%. Uma representação simples do ponto de solda é indicada no caso de modelos completos e para aplicações na indústria, alguns fatores, tais como a modelagem da ZTA, podem ser suprimidos. Uma breve descrição do processo de soldagem a ponto, alguns tópicos da análise de elementos finitos não-linear e algumas características do LS-DYNA, além das conclusões e recomendações para trabalhos posteriores, completam este estudo. / Resistance spot welding is the most common jointing process of parts used in automotive industry. Several methods to represent spot welds are available, and the purpose of this work is to compare two different methods of spot weld modeling through a correlation study, taking into consideration the maximum force withstood by the welded joint, between virtual and lab test results. These spot weld representation methods are beam and solid elements. The tests used in this correlation study load the specimens under pure shear and pure normal forces. And in order to complete this correlation study, the understanding of the failure modes and criteria is also necessary. The specimens finite element models generated to be tested under purê shear and normal conditions are detailed by the presentation of materials, elements contact formulations and loading types used. These models were analyzed by the LS-DYNA, which is general purpose finite element code for analyzing the large deformation dynamic response of structures. As well, the physical tests and used specimens are detailed, and the results from the tests are compared to the results obtained in the simulations. Taking the obtained results, the best correlation was found using solid elements to represent the spot weld, due to differences between the physical and the virtual results about 4%. A simple representation of the spot weld is indicated in case of a complete model, and for the industry application, some factors such as the HAZ, may be suppressed. A brief description of the spot welding process, some topics of the non-liner finite element analysis and some features of the LS-DYNA, and also the conclusions and recommendations for future works, complete this study.

[pt] No novo mapa das mediações, onde se reconhece o papel essencial dos mei-os de comunicação no Brasil, especialmente a televisão, como espaços-chave de produção cultural, ao lançar um olhar para os textos das chamadas da telenovela e para a forma como estas são veiculadas pela mídia de chamadas, percebemos uma possibilidade de criar um sentido para a trama dessa telenovela, que é estruturada de forma seriada, continuada, por um longo período de até oito meses. Esta tese se propõe a demonstrar como, a partir da produção dos textos de chamadas de uma telenovela veiculados por um mecanismo estratégico mercadológico, usado pela emissora Rede Globo de televisão, que é a mídia de chamadas, constrói-se a tessi-tura de uma micro narrativa dos temas desenvolvidos pela narrativa do folhetim eletrônico ao longo de sua exibição. Para empreender essa pesquisa foram anali-sados todos os textos das chamadas produzidas para o lançamento e a manutenção da telenovela Avenida Brasil, último fenômeno de audiência, também com grande repercussão no ambiente digital, veiculada pela Rede Globo em 2012. / [en] In the new map of mediation, where the essencial role of communication media is recognized, in Brazil especially the television, as key spaces of cultural production, by looking at the texts of de telenovela s so-called TV spot teasers and the way they are broadcast by the TV spot teasers media, one recognizes the possibility of creating sense for this telenovela s plot, which is structured in a se-rial, continued, way for a long period up to eight months. This thesis proposes to demonstrate how, starting from the production of a telenovela s TV spot teasers text broadcast by a strategical market-oriented mechanism, used by the Rede Glo-bo television network, which is the TV spot teasers media, one architects the composition of a micro narrative of the themes developed by the telenovela s nar-rative throughout its exhibition. To undertake this research we analyzed all the texts of the TV spot teasers produced for the release and maintenance of the telenovela Avenida Brasil (translated as Brazil Avenue for the international mar-ket), last audience phenomenon, also with considerable repercussion on the digital environment, broadcast by Rede Globo in 2012.

Refill Friction Stir Spot Welding (RFSSW) is a new solid-state joining technology, which is suitable for joining similar and dissimilar overlap sheets connections, particularly in aluminium and magnesium alloys. This welding method is expected to have wide applications in joining of body parts in the automotive industry. In the present study, RFSSW has been used to join 1.0 mm gauge sheets of two material combinations: similar AA6111-T4 automotive aluminium alloy joints and a dissimilar aluminium AA6111-T4 to magnesium AZ31-H24 alloy combinations. The performance of the joints was investigated in terms of the effect of the welding parameters (including tool rotation rate, sleeve plunge depth, and welding time etc.) to improve current understanding and allow optimisation of the process for short welding-cycles when joining similar and dissimilar light alloys. The results of the investigations on similar AA6111 welds showed the ability to use a wide window of process parameters that resulted in joints with a successfully refilled keyhole and flat weld surface, even when using a welding time as short as 0.5 s. The joints in the as-welded condition showed strengths as high as 4.2 kN, when using welding parameters of 1500 rpm, 1.0 mm with a range of welding times from 0.55 to 2.0 s. All joints showed a nugget pull-out failure mode when using a sleeve plunge depth of 0.8 mm or more, as a result of increasing the joint area. The strength of the joints further improved and reached peak loads of 5.15 and 6.43 kN after natural and artificial ageing, respectively, for welds produced using optimised welding parameters of a 2500 rpm tool rotation rate, a 1.5 s welding time and a 1.0 mm plunge. This improvement in strength resulted from the improvement in the local mechanical properties in the HAZ and other regions, which results from a minimal HAZ due to the rapid weld cycle and the re-precipitation of GPZs and clustering on natural ageing, or β on artificial ageing. A modification to the RFSSW process was developed in this project to solve the problems faced when dissimilar welding Mg to Al. This modified process involved adding a final brief pin plunge stage to consolidate refill defects and it was successful in producing nearly defect-free joints with improved mechanical properties, using a wide range of the process parameters. The average peak load of the joints increased with increasing tool rotation rate, to reach a maximum value at 2500 rpm due to eliminating the weld defects by increasing the material plasticity. However, increasing the tool rotation rate further to 2800 rpm led to a decrease in the average peak failure load due to eutectic melting at the weld interface. The optimum welding condition was thus found to be: 2500 rpm, 1.0 s, and 1.0 mm, which gave an average peak failure load of 2.4 kN and average fracture energy of 1.3 kN.mm. These values represent an improvement of about 10 % and 27 %, respectively, compared to welds produced with the conventional RFSSW process, and about 112 % and 78 % of the Mg-Mg similar joints produced using the same welding conditions. A FE model developed in this project was successful in increasing understanding of the behaviour of the RFSSW joints when subjected to lap tensile-shear loading. The stress and strain distribution in the modelled samples showed that the highest concentration occurring in the region of the confluence of the SZ with the two sheets. With increasing extension, these regions of highest stress and strain propagated to the outer surfaces of the two sheets and then annularly around the weld nugget. This annular ring of high strain concentration agreed well with the failure path and results in the full plug pull-out fracture mode shown by the experimentally tested samples. The predicted force-extension curves showed high agreement with the experimental results, especially when including the effect of the hook defect and correction of compliance in the experimental results.

O presente trabalho teve como objetivo desenvolver uma metodologia prática e confiável para previsão de vida à fadiga de juntas soldadas a cordão pelo processo MIG/MAG, quando estas estão submetidas a carregamentos cíclicos combinados. A máxima tensão linear no pé da solda, região típica de início de trinca, conhecida como hot spot foi utilizada para prever a vida através do método de Fadiga de Alto Ciclo S x N (Tensão x Vida), largamente empregado em códigos de projeto de estruturas soldadas. O Método dos Elementos Finitos foi utilizado para determinação das tensões estruturais resultantes do carregamento e das descontinuidades geométricas presentes nos cordões de solda. A análise de fadiga foi efetuada em ambiente virtual, através de um software (programa) de fadiga capaz de importar as tensões atuantes na região da solda para cada carregamento, combinando-as e obtendo assim a vida à fadiga decorrente da somatória dos diferentes tipos de carregamento ao qual a junta foi submetida. As propriedades monotônicas e cíclicas dos materiais da junta foram obtidas da literatura e de um extenso banco de dados disponível no software de fadiga. Estas propriedades foram ajustadas com base em ensaios de laboratório nas juntas investigadas. A medição ou modelagem das tensões residuais inerentes ao processo de soldagem não fazem parte do escopo deste trabalho. Contudo, os efeitos térmicos e metalúrgicos resultantes do processo de soldagem, como distorções, tensões residuais, variações microestruturais e propriedades mecânicas foram considerados de forma indireta, através da correção das curvas de fadiga nos corpos de prova investigados. Corpos de prova do tipo tubo-placa foram submetidos a carregamentos cíclicos combinados (flexão e torção) de amplitude constante. O resultado da análise virtual de durabilidade foi, portanto, calibrado com base nestes experimentos e curvas disponíveis em códigos de projeto de fadiga como BS7608 e Eurocode 3. A aplicabilidade deste método numérico-experimental e suas contribuições para a garantia da Integridade Estrutural do projeto de juntas soldadas são apresentadas. Seus desafios e melhorias são por fim discutidos. / The main purpose of this work is to develop a practical and robust methodology to evaluate the fatigue life in seam weld joints fabricated with GMAW process when subjected to combine cyclic loading. The maximum linear stress at the typical crack initiation region, better known as hot spot stress, was used to calculate the fatigue life through high cycle fatigue method S x N (Stress x Life), widely used in design codes for the life assessment of welded structures. The Finite Element Method (FEM) was used to obtain the structural stresses distribution due the external loading and geometric discontinuities very common in seam weld joints. The fatigue analysis was conducted in virtual environment. The FEM stress results from each loading were imported to fatigue code FE-Fatigue and combined to perform the fatigue life prediction. The monotonic and cyclic properties of the joint materials were obtained in the literature and from the fatigue software database. These properties were adjusted based on laboratory fatigue tests in the investigated welded joints configurations. The measurement or modeling of the residual stresses resulted from the welded process is not part of this work. However, the thermal and metallurgical effects, like distortions and residual stresses, were considered indirectly through fatigue curves corrections in the samples investigated. A tube-plate specimen was submitted to combine cyclic loading (bending and torsion) with constant amplitude. The virtual durability analysis result was calibrated based on these laboratory tests and design codes such as BS7608 and Eurocode 3. The feasibility and application of the proposed numerical-experimental methodology and contributions for the welded joints structural integrity design are presented. The challenges and improvements are finally discussed.

A crescente exigência do setor automotivo por maior produtividade com redução de custos, aliada aos novos materiais que compõe as carrocerias e seus revestimentos, levou ao estudo da correlação da resistência dinâmica, força de expansão térmica e indentação, com a formação do ponto de solda, a fim de qualificar e controlar a soldagem por resistência a ponto. Para o estudo ter validade prática a qualificação da solda, os parâmetros de soldagem e corpos de prova foram definidos conforme norma AWS-SAE D8-9M 2002. Os corpos de prova para os ensaios foram retirados das chapas que compõe uma carroceria veicular. O cabeçote de soldagem foi projetado e construído seguindo os padrões de máquinas industriais. Os instrumentos de medição foram desenvolvidos especificamente para atender as condições industriais da soldagem por resistência. Os ensaios simularam as variações de parâmetros e produção dos pontos de solda conforme ocorre na indústria automobilística. As análises foram realizadas através da comparação gráfica e matemática do comportamento das grandezas medidas com a qualificação dimensional do ponto. A correlação, apresentada, distingue chapas de espessuras diferentes, materiais de revestimento, variação de parâmetros, erros de posicionamento (ponto torto), expulsão de material (flash), variações na formação do ponto (undersize) e o mais importante, desgaste dos eletrodos. Esta correlação permite criar um sistema de qualificação e controle do ponto de solda. / The growing demand from the automotive sector for greater productivity, reduced costs, combined with new materials that make up their bodies and coats, led to the study of the correlation of the dynamic resistance, thermal force expansion and indentation, with the formation of the spot weld, in order to qualify and control the resistance spot welding. For the study to be valid practicing the qualification of spot weld, welding parameters and specimens were define according AWS - 9M SAE D8 2002 standard. The specimens for testing were removed from the sheets that make up a vehicle body. The welding head was designed and built according the standards of industrial machinery. Measuring instruments were developed specifically to meet the conditions of industrial resistance welding. The tests simulated the variations of parameters and production of welding spots as occurs in automotive industry. The analyzes were performed through the graphical comparison and mathematical behavior of measured quantities with dimensional spot qualification. The correlation presented distinguishes sheets of different thicknesses, coating materials, variation of parameters, positioning errors (crooked point), expulsion of material (flash), variations in spot formation (undersize) and most important, wear of the electrodes. This correlation allows to create a system of qualification and control of the spot welding.

This thesis is a report concerning the practical aspects on exhibition techniques. During an internship at the National History Museum in Stockholm I participated in the work with their new, temporary exhibition Gränslöst - en global utställning på Historiska museet [Boundless - a global voyage of discovery at the National Historical Museum]. I was part of a small project team of museum staff. The purpose of this exhibition was to show how cultures have met and influenced each other throughout history. The National Historical Museum has in its collections and exhibitions several examples of objects that has been imported from or inspired by other cultural areas. The primary goal was to give the visitors a new perspective, to show that these “Swedish” objects have a long history of trading and cultural exchange. Additionally, Gränslöst is an attempt to draw the visitors into the permanent exhibitions; it is not an independent production and shares some characteristics with so- called “Hot spot” exhibitions. My ambition is to describe the planning, implementation and delivery of the exhibition and highlight problems and solutions that arise during that process.

Welding is one of the most applied technics in the world for joining steel. Welds are liable to the phenomenon of fatigue, which is, primarily, the formation of a crack and consequently reduction of strength due to the action of time varying loads. Fatigue is one of the main causes of failure in steel structures. The aim of this thesis is to do static and dynamic analyses of a forestry crane with the purpose of using the analyses to determine the lifetime due to fatigue of welded components. Two methods for fatigue assessment are used in this work, the Hot-Spot Method and the Notch Stress Method. The first boom, which is a key component for the crane, is analyzed in a Finite Element Method (FEM) software. The found principal stress in accordance with the notch stress method in the first boom is ±165 MPa for the analyzed load case, rendering in a stress range of 330 MPa. The fatigue strength class FAT-225 (m=3), leads to an expected number of 633000 cycles, with a probability of survival of 97,7% for this case. / Svetsning är en av de vanligaste teknikerna för sammanfogning av stål. Svetsar är känsliga för utmattning. Utmattningsfenomenet består primärt av en initial dislokation som genom tidsvarierande belastning formar en spricka som växer och därmed reducerar styrkan i konstruktionen. Utmattning är en av de vanligaste orsakerna till skador i stålkonstruktioner. Målet med detta arbete är att genomföra både statiska och dynamiska analyser av en skogsmaskins kran i avseende att bestämma utmattningslivslängden för dess svetsade konstruktioner. Två metoder för utvärdering används i detta arbete, hot-spot-metoden och notch-stress-metoden. Kranens första bom (lyftarmen) som är en huvudkomponent i kranen analyseras med hjälp av ett Finita Element program i enlighet med notch-metoden. Högsta funna spänningsvariationen i första huvudspänningsriktningen var ±165 MPa för ett av de analyserade lastfallen. Utmattningsklass FAT 225 (m=3) ger en uppskattning om utmattningslivslängd på 633000 cykler med en sannolikhet för överlevnad på 97.7% i detta fall.

Das Eingliedern festsitzender kieferorthopädischer Apparaturen macht das vorherige Anrauhen des Schmelzes notwendig. Das Ziel dieser Arbeit bestand darin zu ermitteln, ob ein zeit- und ausdehnungsbezogen überschüssiges kieferorthopädisches Ätzen des Schmelzes über die Fläche des zu befestigenden Brackets hinaus mit 30% Phosphorsäure zu einem erhöhten Risiko für White-Spot-Läsionen beitragen kann. Zusätzlich wurde getestet, inwieweit die Faktoren Zahnreinigung, Ätzdauer und Zeitdauer auf die mögliche Entstehung von White-Spot-Läsionen Einfluss nehmen. Für diese in-vitro-Studie wurden 90 extrahierte menschliche obere mittlere und seitliche Frontzähne verwendet. Die Zahnkronen mit einem Durchmesser von mindestens fünf mm wurden in Kunststoff eingebettet und mit Schleifpapier poliert. Es wurde jeweils eine Baseline-Messung mittels Quantitativer lichtinduzierter Fluoreszenz (QLF) durchgeführt, während der die Referenzbereiche für die nachfolgenden Messungen festgelegt wurden. Anschließend wurden die 90 Prüfkörper randomisiert in sechs Gruppen aufgeteilt. Drei Gruppen wurden täglich einer standardisierten Reinigung unterzogen und jeweils zu Versuchsbeginn 30 Sekunden, 15 Sekunden oder 0 Sekunden mit 30% Phosphorsäure angeätzt. Die Prüfkörper der anderen drei Gruppen wurden keiner Reinigung unterzogen und ebenfalls 30, 15 oder 0 Sekunden angeätzt. Während 42 aufeinanderfolgenden Tagen wurden alle Prüfkörper einem pH-Zyklus unterzogen: Die Demineralisation erfolgte für 60 Minuten. Hierauf folgte eine 120 minütige Remineralisation. Dieser Zyklus wurde drei Mal pro Tag durchgeführt. Nach dem letzten Zyklus wurden drei Gruppen mithilfe einer Zahnputzmaschine, die mit eingespannten Bürstenköpfen und einem Gemisch aus künstlichem Speichel und fluoridhaltiger Zahnpasta arbeitete, standardisiert gereinigt. Nach 2, 7, 14, 21 und 42 Tagen wurden die Fluoreszenzwerte der Schmelzoberflächen mittels QLF ermittelt. Bei der Auswertung der Messergebnisse zeigte sich ein signifikanter Einfluss  (p < 0,01) aller drei Faktoren (Reinigung, Ätzdauer, Versuchszeit) auf die Fluoreszenzwerte. Die Fläche der Demineralisation wurde nur durch die Ätzdauer signifikant beeinflusst. Der Einfluss der längeren Ätzdauer (30 Sekunden) verstärkte sich insbesondere bei den ungereinigten Prüfkörpern. Der DeltaQ-Wert wurde lediglich durch die verstrichene Versuchszeit in Kombination mit 30 sekündigem Ätzen signifikant beeinflusst (p < 0,02). Werden angeätzte Schmelzbereiche nicht von Bonding oder Bracket bedeckt, ist somit mit einer verstärkten Entstehung von White-Spot-Läsionen zu rechnen. Folglich ist bei der kieferorthopädischen Bracketbefestigung darauf zu achten, die Ätzfläche auf die Fläche des zu klebenden Brackets zu beschränken und Ätzzeiten von 15 Sekunden nicht zu überschreiten.