Submitted by Vitor Frango (vitorpollo@yahoo.com.br) on 2014-03-10T14:51:32Z No. of bitstreams: 1 Dissertação Vitor Frango.pdf: 552731 bytes, checksum: be98ecc9e672434717e644336c1c4c32 (MD5) / Approved for entry into archive by JOANA MARTORINI (joana.martorini@fgv.br) on 2014-03-10T14:58:20Z (GMT) No. of bitstreams: 1 Dissertação Vitor Frango.pdf: 552731 bytes, checksum: be98ecc9e672434717e644336c1c4c32 (MD5) / Made available in DSpace on 2014-03-10T15:43:21Z (GMT). No. of bitstreams: 1 Dissertação Vitor Frango.pdf: 552731 bytes, checksum: be98ecc9e672434717e644336c1c4c32 (MD5) Previous issue date: 2014-01-30 / This study aims to estimate the control premium in the Brazilian stock market, based on the price differential between classes of stocks with different voting rights. In Brazil for the period from 01/07/2003 to 28/06/2013 the average control premium is positive, a result that differs from those found in previous studies, where the control premium is negative. This paper investigates the determinants that imply this voting shares valuation relative to the non-voting share. Particularly, the paper analyzes the impact of the liquidity, dividends differential, the negative impact of the extension of the right of the Tag Along for non-voting shares, the negative impact of ADR companies, a negative impact when firms are classified with upper level corporate governance in BMF&Bovespa. Finally, the positive impact when the major shareholder holding more than 50% of the voting shares and government holding more than 20% of voting shares. / Este trabalho visa estimar o prêmio por controle no mercado acionário brasileiro, com base no diferencial de preços entre espécies de ações com direitos diferenciados de voto. No Brasil para o período de 01/07/2003 a 28/06/2013 a média do prêmio por controle foi positivo, resultado que difere dos encontrados em estudos anteriores, onde o prêmio por controle é negativo. Este trabalho investiga os determinantes que implicam na valorização da ação ordinária em relação à ação preferencial. Em particular, o trabalho analisa os impactos da liquidez, do diferencial dos dividendos, o impacto negativo da extensão do direito do Tag Along para ações preferenciais, o impacto negativo das empresas que possuem ADR, impacto negativo quando a empresas está classificada com nível superior de governança corporativo da BMF&Bovespa e pro fim, o impacto positivo para quando o acionista majoritário detêm mais de 50% das ações ordinárias e da participação do governo nas empresas que possui 20% das ações ordinárias.

Medical rescue workers are routinely employed in emergency medicine. In emergencies where a large number of people are affected, knowledge of medical disaster practices is necessary. Within a second, the intervening crews should switch to another mode with a different approach to the patients than they use daily. The diploma thesis has set the following goals: to map the level of knowledge of medical rescuers in the field of emergency response with mass health impairment, their personal experience and, last but not least, education and training. Three research questions were asked to reach these objectives. What are the skills of medical rescuers in dealing with emergency health issues with mass health impairment? How do medical rescuers assess incidents with mass health impairment at which they were present and what is their attitude towards training and education in the field of dealing with emergencies with mass health impairment? The research part consists of interviews with two groups of participants: eight medical rescuers of the Medical Rescue Service of the Pardubice Region and the same number of medical rescuers of the Medical Rescue Service of the South Bohemian Region. These interviews supplemented the information provided by the crisis preparedness managers of both medical rescue services. The survey used a qualitative research strategy, conversations were recorded on a dictaphone, then processed into categories, subcategories, tables and evaluated. From the results of the work, a great difference in the knowledge of health rescuers in the field of emergency health problems can be seen. Participants of the South Bohemian Medical Emergency Rescue Service showed shortcomings in both the basic terminology and the solving of extraordinary events in particular, from reporting the situation with a report form to the classification of the disabled. On the contrary, the majority of participants in the Paramedical Rescue Service of the Pardubice Region would be able to file an initial emergency report and categorize and transport patients correctly. On the basis of the evaluated results, this difference in knowledge can be attributed mainly to the frequency and content of the training provided. In the case of South Bohemian participants, we can find the cause in the absence of practical training. The alarming results were noted in the participants' ability to use the START sorting method, from all of the sixteen respondents only one was able to answer there. The elaborated case report of an extraordinary event points to the fact that theoretical shortcomings can subsequently be transferred into practice.

Made available in DSpace on 2014-06-11T19:26:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-07-20Bitstream added on 2014-06-13T18:54:09Z : No. of bitstreams: 1 jovino_dfr_me_jabo.pdf: 637580 bytes, checksum: dc449a2a52a9354da867effed1512963 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Sob condições de estresse hídrico, a cana-de-açúcar pode sofrer mudanças fisiológicas e bioquímicas, tais como diminuição nas atividades fotoquímicas, redução da fixação de CO2 e acúmulo de osmólitos e osmoprotetores. O objetivo deste trabalho foi identificar, através da técnica de macroarranjo de cDNA, o perfil de expressão de genes promotores das diferentes vias metabólicas em palmitos da variedade de cana-de-açúcar SP80-3280 submetidas ao estresse hídrico nos dias 5, 9, 13 e 17 após o início da condição de supressão de água, sendo considerado o dia 1 como controle. Os resultados do macroarranjo mostraram que as proteínas mais expressas sob déficit hídrico pertencem a quatro categorias das quais as ESTs mais importantes foram selecionadas. As quatro categorias descritas abaixo estão discutidas neste trabalho. As ESTs da via do metabolismo de açúcar e amido (invertase de parede celular (INV), sacarose fosfato sintase (SFS), sacarose fosfato fosfatase (SFF), trealose fosfato sintase (TFS), trealose fosfato sintase/fosfatase (TFS/F) e hexoquinase (HXQ)) pertencem a categoria de bioenergética. Colina monooxigenase (CMO) e betaína aldeído desidrogenase (BADH) as quais pertencem a via do metabolismo de glicina betaína, foram selecionadas a partir da categoria do metabolismo secundário. A terceira categoria compreende as ESTs do metabolismo de aminoácido (D-pirrolina-5- carboxilato sintase (P5CS), ornithina -aminotransferase (OAT) e prolil 4-hidroxilase (PH)) as quais pertencem a via da biossíntese de prolina, e a quarta categoria, resposta ao estresse, compreende as ESTs aleno oxido sintase (AOS), aleno oxido ciclase (AOC) e lipoxigenase (LOX), pertencentes a via de biossíntese do jasmonato. / Under water deficit, sugarcane undergoes physiological and biochemical alterations such as photochemical activity reduction, CO2 fixation reduction and the accumulation of osmolytes and osmoprotectants. This work was undertaken to identify the gene expression profile in sugarcane (var. SP80-3280) under water deficit through the cDNA macroarray technique. Leafroll tissues were collected from plants subjected to 5, 9, 13 and 17 days of water restriction, and day 1 was used as control. Macroarray results showed that most proteins expressed under water restriction belong to four categories, from which the most important ESTs were selected. The four categories described below are discussed in this work. Sugar and starch metabolism pathway ESTs (cell wall invertase (CWI), sucrose phosphate synthase (SPS), sucrose phosphate phosphatase (SPP), trehalose phosphate synthase (TPS), trehalose phosphate synthase phosphatase (TSP/P) and hexoquinase (HXQ) were selected from the bioenergetics category. Choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH), which belong to the glycine betaine metabolism pathway, were selected from the secondary metabolism category. The third category comprised the amino acid metabolism ESTs D- pyrroline-5-carboxylate synthase (P5CS), Ornithine - aminotransferase (OAT) and Prolyl 4-hydroxylase (PH), which belong to the proline biosynthesis pathway, and the fourth category, stress response, comprised the ESTs for Allene oxide synthase (AOS), Allene oxide cyclase (AOC) and Lipoxygenase (LOX), which belong to the jasmonate biosynthesis pathway.

abstract: The critical infrastructures of the nation are a large and complex network of human, physical and cyber-physical systems. In recent times, it has become increasingly apparent that individual critical infrastructures, such as the power and communication networks, do not operate in isolation, but instead are part of a complex interdependent ecosystem where a failure involving a small set of network entities can trigger a cascading event resulting in the failure of a much larger set of entities through the failure propagation process. Recognizing the need for a deeper understanding of the interdependent relationships between such critical infrastructures, several models have been proposed and analyzed in the last few years. However, most of these models are over-simplified and fail to capture the complex interdependencies that may exist between critical infrastructures. To overcome the limitations of existing models, this dissertation presents a new model -- the Implicative Interdependency Model (IIM) that is able to capture such complex interdependency relations. As the potential for a failure cascade in critical interdependent networks poses several risks that can jeopardize the nation, this dissertation explores relevant research problems in the interdependent power and communication networks using the proposed IIM and lays the foundations for further study using this model. Apart from exploring problems in interdependent critical infrastructures, this dissertation also explores resource allocation techniques for environments enabled with cyber-physical systems. Specifically, the problem of efficient path planning for data collection using mobile cyber-physical systems is explored. Two such environments are considered: a Radio-Frequency IDentification (RFID) environment with mobile “Tags” and “Readers”, and a sensor data collection environment where both the sensors and the data mules (data collectors) are mobile. Finally, from an applied research perspective, this dissertation presents Raptor, an advanced network planning and management tool for mitigating the impact of spatially correlated, or region based faults on infrastructure networks. Raptor consolidates a wide range of studies conducted in the last few years on region based faults, and provides an interface for network planners, designers and operators to use the results of these studies for designing robust and resilient networks in the presence of spatially correlated faults. / Dissertation/Thesis / Doctoral Dissertation Computer Science 2016

Jahresbericht der Universitätsbibliothek Chemnitz - Berichtsjahr 2007 / Annual report of the University Library of Chemnitz in 2007

The aim of this thesis is to develop an educational methodology for the following languages: Markdown, HyperText Markup Language (HTML), eXtensible Markup Language (XML) and Scalable Vector Graphics (SVG); and to verify the methodology at Czech elementary schools on the lower secondary level. An integral part of the methodology is also an introduction to the history and development, as well as the theory of syntax of the mentioned markup languages, necessary for teaching according to this methodology. The methodology uses specific examples, on which the students can verify their newly acquired knowledge in practice. The verification of the methodology took place in the form of teaching at two Czech elementary schools. The teaching took place in the seventh grade within the compulsory elective subject of informatics, in the ninth grade within a compulsory subject. The expected differences in understanding the markup languages education in favor of the ninth year have not been confirmed. The motivation of the students proved to play much greater role than their age. The verification of the methodology has shown that it is necessary to pay careful attention to the precise formulation of the task assignments and guidelines to the students, to avoid confusion and substitution of the source code of the...

Transmissible spongiform encephalopathies (TSEs) or prion diseases are a class of fatal brain disorders better known as Creutzfeldt-Jacob Disease (CJD) in humans, bovine spongiform encephalopathy (BSE) in cattle, scrapie in sheep, and chronic wasting disease (CWD) in deer and elk. The infectious agent responsible for these diseases is a misfolded prion protein capable of catalyzing a conformational change in normal cellular prion proteins (PrPC) into aberrant disease-causing structural isoforms (PrPSc). Although the etiological agent for TSEs has clearly been defined as PrPSc, there are important gaps in our understanding of how these proteins target and invade brain tissue. It remains to be established how ingested PrPSc ultimately reach the brain and also to understand why these tissues are particularly targeted, notwithstanding that several other tissues highly express prion proteins. Certain viruses, retroviruses in particular, efficiently hijack host proteins and can carry these proteins with them when they are released from a cell. Several lines of evidence have shown that prions and retroviruses can interact and associate at various stages of the retroviral replication cycle. Of special interest is that most retroviruses can cross the blood-brain barrier and could therefore deliver host-derived proteins to neuronal cells. In view of these observations, this thesis investigates whether retroviruses can act as vectors to capture prions from an infected cell and deliver them to a susceptible target cell. In this work, I have cloned human and mouse prion cDNAs from PBMCs and the murine cell line NIH 3T3. Either a FLAG epitope tag or the eGFP reporter protein cDNA was inserted into a region of the prion cDNA that is predicted to be amenable to such genetic insertions without affecting protein folding or expression. I then confirmed using both fluorescent and confocal microscopy and that the recombinant proteins had a similar cell distribution to the endogenous prion protein. Using Western blot analysis, I then showed that endogenous and overexpressed prion proteins can be detected in co-transfected cells producing HIV and murine leukemia virus (MLV) retroviral particles. Finally, I went on to show that prions are also present at high levels in HIV and MLV retroviral particles released from these cells. This work constitutes the first step in determining whether retroviruses can act as vectors for prion dissemination. Establishing a strong and clear association between retroviruses, pathogenic prions and prion disease would provide the rationale for preventive measures to be taken directly against retroviruses in order to protect humans and animals that have been newly exposed to PrPSc-infected products or those who are genetically predisposed to develop prion diseases. Anti-retroviral drugs could also be potentially used to delay disease progression and reduce prion transmission in human and animal tissues. The availability of such a treatment would constitute a significant advancement because there is currently no cure or treatment for prion diseases.

Groat oil content and composition are important quality traits in oats (Avena sativa L). These traits are controlled by many genes with additive effects. The chromosomal regions containing these genes, known as quantitative trait loci (QTL), can be discovered through their close association with markers. This study investigated total oil content and fatty acid components in an oat breeding population derived from a cross between high oil ('Dal') and low oil ('Exeter') parents. A genetic map consisting of 475 DArT (Diversity Array Technology) markers spanning 1271.8 cM across 40 linkage groups was constructed. QTL analysis for groat oil content and composition was conducted using grain samples grown at Aberdeen, ID in 1997. QTL analysis for multiple agronomic traits was also conducted using data collected from hill plots and field plots in Ottawa, ON in 2010. QTLs for oil content, palmitic acid (16:0), stearic acid (18:0), oleic acid (18:1), linoleic acid (18:2) and linolenic acid (18:3) were identified. Two of the QTLs associated with oil content were also associated with all of the fatty acids examined in this study, and most oil-related QTL showed similar patterns of effect on the fatty acid profile. These results suggest the presence of pleiotropic effects on oil-related traits through influences at specific nodes of the oil synthesis pathway. In addition, 12 QTL-associated markers (likely representing nine unique regions) were associated with plant height, heading date, lodging, and protein content. The results of this study will provide information for molecular breeding as well as insight into the genetic mechanisms controlling oil biosynthesis in oat.

The diploma thesis deals with optimization of material flow using RFID technology in Bosch Diesel company at the Expedition department. The theoretical part defines the basic concepts related to logistics, RFID technology and material flows. The analytical section describes the process of packing, data confirmation and dispatching CP4 pumps to the customer. Based on the analytical part, there are suggestions that optimize the time-consuming process and reduce the error rate of the recorded data to the system.

The bacterium Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection worldwide. When left untreated, chlamydial infections can lead to severe complications, such as infertility. Lack in current prevention and management due to its asymptomatic course of infection highlight the need for an effective vaccine against chlamydia. There is no vaccine at present to protect against chlamydia, but research is ongoing. A research group at Örebro University has developed a protein antigen candidate. This project focused on the production of the candidate, here called Protein X, for preclinical trials. This included optimising production in Escherichia coli to maximise formation of soluble protein, optimising purification, buffer exchange and removal of His-tag. It was found that formation of soluble protein was favoured in lower expression temperatures. Furthermore, purification was performed on soluble and insoluble protein fractions using immobilised metal affinity chromatography. However, issues with inefficient binding to the resin and purity could not be solved and further optimisation is needed. Buffers were tested to find a suitable buffer for preclinical experiments, but the protein precipitated in all buffers. It was however found that protein from the insoluble fraction dissolved in pure water. Lastly, removal of the His-tag was performed with a non-enzymatic method that utilises nickel ions instead of expensive proteases. Efficient removal was however not achieved and enzymatic methods may be considered instead. In conclusion, this project highlighted issues in the production of Protein X and may guide the research group towards improving this process for efficient preclinical preparations.