In dieser Arbeit wurde die Affinitätsreifung des murinen anti-c-myc-Peptid-Antikörpers 9E10 analysiert. Hierfür wurden Fab-Fragmente mit Keimbahnrückmutationen gentechnisch hergestellt und in ihrem Bindungsverhalten zum humanen c-myc-Peptid charakterisiert. Das von 9E10 erkannte Epitop besitzt die Aminosäuresequenz EQKLISEEDLLRKR mit den darin sehr selektiv erkannten Schlüsselpositionen LISEXXL.Der 3300-fache Affinitätsgewinn während der 9E10-Reifung kommt sowohl durch eine Zunahme der Assoziations- als auch durch eine Abnahme der Dissoziationsgeschwindigkeit des Komplexes zustande. Der Affinitätsgewinn resultiert weniger aus zusätzlichen Kontakten des Antikörpers zum Peptid, sondern vor allem aus der Beeinflussung der Konformation und/oder der Flexibilität der an der Bindung beteiligten CDRs. Die außergewöhnlich lange CDR-H3 liefert einen wesentlichen Beitrag zur Affinitätsreifung. Die variable leichte Domäne dient dabei mit der langen CDR-L1 und -L3 als eine Bindungsplattform für die flexible CDR-H3. Änderungen in der Spezifität von 9E10 sind vorrangig auf die Reifung der variablen schweren Domäne zurückzuführen. Dabei ist die selektive Erkennung der Schlüsselpositionen im Peptid im Anfangsstadium der Affinitätsreifung von 9E10 stark ausgeprägt. / In this work the affinity maturation of the murine anti c-myc-peptide antibody 9E10 was analysed. Therefore Fab fragments with reversed mutations directed towards germline genes were genetically produced and characterised for their binding to the human c-myc peptide. The epitope recognized by 9E10 consists of the amino acid sequence EQKLISEEDLLRKR of which the key positions LISEXXL are very selectively recognized. The maturation of 9E10 leads to a 3300-fold higher affinity, which is achieved by a faster association as well as by a slower dissociation of the complex. For the gain in affinity formation of additional contacts to the peptide is less important than conformational and/or flexibility changes of the CDRs which are involved in binding. The exceptionally long CDR-H3 contributes essentially to the affinity maturation. The variable light domain serves thereby with its long CDR-L1 and -L3 as a binding platform for the flexible CDR-H3. Changes in specificity of 9E10 are primarily due to maturation of the variable heavy domain. Selective recognition of the key positions in the peptide is already highly pronounced in the initial stage of affinity maturation of 9E10.

除了文字訊息，張貼相片也是臉書使用者常用的功能，這些上傳的照片種類繁多，可能是自拍照、風景照、或食物照等等，本論文的研究以影像分析為出發點，探討相片內容跟發佈者間之關係，希望藉由相片獲得的資訊，輔助分析使用者樣貌。 本研究共收集32位受測者上傳至臉書的相片，利用電腦視覺技術分析圖像內容，如人臉偵測、環境識別、找出影像上視覺顯著的區域等，藉由這些工具所提供的資訊，將照片加註標籤，以及進行自動分類，並以此兩個層次的資訊做為特徵向量，利用階層式演算法進行使用者分群，再根據實驗結果去分析每一群的行為特性。 透過此研究，可對使用者進行初步分類、瞭解不同的使用者樣貌，並嘗試回應相關問題，如使用者所張貼之相片種類統計、不同性別使用者的上傳行為、 依據上傳圖像內容，進行使用者樣貌分類等，深化我們對於臉書相片上傳行為的理解。 / Apart from text messages, photo posting is a popular function of Facebook. The uploaded photos are of various nature, including selfie, outdoor scenes, and food. In this thesis, we employ state-of-the-art computer vision techniques to analyze image content and establish the relationship between user profile and the type of photos posted. We collected photos from 32 Facebook users. We then applied techniques such as face detection, scene understanding and saliency map identification to gather information for automatic image tagging and classification. Grouping of users can be achieved either by tag statistics or photo classes. Characteristics of each group can be further investigated based on the results of hierarchical clustering. We wish to identify profiles of different users and respond to questions such as the type of photos most frequently posted, gender differentiation in photo posting behavior and user classification according to image content, which will promote our understanding of photo uploading activities on Facebook.

Text in German with German and English summaries / In times of global crisis, eschatological questions gain relevance. The Old Testament concept of HàWH:J moWJ has primary bearing on Christian eschatological expectations. This study examines the discourse of the Day of Yahweh of Amos and Isaiah in the context of their message. Concept and origin of this discourse are controversial. A review of research shows that the current understandings do not adequately answer questions about the origin of this discourse. Both the definitions of “Day of Yahweh” passages and the certitude that HàWH:J moWJ is a technical term seem problematic. Analysis of Amos 5:18-20 shows that, contrary to commonly accepted understanding, a nontechnical understanding of the phrase HàWH:J moWJ is sufficient for interpreting the passage. Linguistically similar phrases in Isaiah also merely relate Yahweh’s historical intervention, and do not refer to an established concept. Only later did a tradition complex arise from this prophetic proclamation. / In Zeiten globaler Krisen gewinnen eschatologische Fragestellungen an Aktualität. Zentrale Bedeutung für die christliche Zukunftserwartung hat der Tag des Herrn, dessen alttestamentlicher Wurzelgrund der HàWH:J moWJ ist. Die Studie untersucht die Rede vom Tag Jahwes bei den Schriftpropheten Amos und Jesaja im Kontext ihrer Verkündigung. Konzept und Herkunft dieser Rede sind umstritten. Ein Forschungsüberblick zeigt, dass die gängigen Erklärungsmodelle die Frage nach der Herkunft dieser Rede nicht ausreichend beantworten. Als problematisch erscheinen die Definitionen von Tag Jahwes-Belegen und die Sicherheit, mit der man die Wendung HàWH:J moWJ als Terminus technicus bezeichnet. Die Untersuchung zu Am 5,18-20 erweist, dass, entgegen dem Common sense der Forschung, ein formales Verständnis der Wendung HàWH:J moWJ zur Interpretation der Rede ausreicht. Ähnliche sprachliche Wendungen bei Jesaja paraphrasieren ebenfalls nur das geschichtliche Eingreifen Jahwes und verweisen nicht auf eine Auseinandersetzung mit einem bestehenden Konzept. Ein Traditionskomplex namens Tag Jahwes erwuchs erst später aus dieser prophetischen Verkündigung. / Biblical and Ancient Studies / M. Th. (Old Testament)

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在這社會網路蓬勃發展之中、網際網路頻寬與速度相繼提昇的資訊年代，結合網路科技所衍生的Flickr網路相簿因應而生。Flickr提供許多API程式讓使用者或有興趣研究的專家學者能透過Flickr所收集及其所探討的議題，來觀察社會網路的變化情形。 社會網路主要是由節點以及節點間彼此相連結所形成，常見的網路模型大致可分為One-mode與Two-mode兩種網路結構，而本文則採用內部同時有兩種類節點、由兩個城市與Tags共同組合而成的Two-mode網路為基礎架構，期望藉此來闡述一個Tags系統分析法，利用Flickr使用者收集、標註之Flickr標記來與世界城市的概念輪廓相連結，透過提取城市語義分配給Flickr上照片的Tags，以及解決Part-Of-Speech （POS）、詞幹還原及雜訊處理…等問題，來達成依據排名結果分析出城市概念輪廓的最終目的。 除此之外，本文還運用了Flickr tag資料來彙整出41個城市的前100名tag，再篩選出前10名的tag，將其與相關的城市歸類一起比較。本文亦使用字詞共現指標（Tag co-occurrence）來計算與該城市的關聯性，再利用此法則來歸納出這兩個城市字詞共同出現的機會，以便於了解城市與城市之間的關連字詞組合。最後，本研究亦透過Flickr網站本身Popular Tags經由分析及匯出標籤雲的結果來與本文之實驗結果相對照，本實驗85%的吻合度驗證了可靠性。 / The Flickr Web Albums was born in the information age of social network growth, internet bandwidth and speed improvement. Users and researchers can observe the changing of social network from topics collected and studied by Flickr using API programs provided by Flickr. The main structure of social network can be distinguished one-mode and two-mode network which is composed by nodes, generally. An approach for world cities concept profiling analysis is developed in this study by conbineing two types of nodes and two cities with tag which is the two-mode network using extracting city semantics for tags assigned to photos on Flickr, solving Part-of-Speech（POS）, Stemming reduction and noise handing by collecting Flickr's tags from Flickr users. The top 100 tags were slected for 41 cities and then top 10 tags for each city were also extracted. The Tag co-occurrence was also applied to analysis the relationship of cities. Then the connection between the cities can be understood by the result of tag co-occurrence opportunities. The 85% accurancy was demonstrated by comparing the result of analysised and exported Popular Tags from Flickr Website service and the result of experiments in this study.

Dans les dernières années, une explosion de la recherche sur les ARN a eu lieue à cause de nombreuses découvertes démontrant l’importance de l’ARN dans plusieurs processus biologiques. Ainsi, de grandes quantités d’ARN sont devenues indispensables au bon déroulement de plusieurs études, notamment pour la biologie structurale et la caractérisation fonctionnelle. Cependant, il existe encore peu de méthodes de purification simples, efficaces, fiables et produisant un ARN sous forme native. Dans les dernières années, le laboratoire Legault a mis au point une méthode de purification par affinité utilisant une étiquette ARiBo pour la purification d’ARN transcrits in vitro par la polymérase à ARN du phage T7. Cette méthode de purification d’ARN a été spécifiquement développée pour maximiser la pureté et le rendement. De plus, elle est très rapide et fonctionne avec plusieurs types d’ARN. Cependant, comme plusieurs autres méthodes de purification, cette méthode produit des ARN avec des extrémités 5′ hétérogènes. Dans ce mémoire, des solutions sont proposées pour remédier au problème d’hétérogénéité en 5ʹ′ des ARN transcrits avec la polymérase à ARN du phage T7 et purifiés par la méthode ARiBo. La première solution consiste à choisir la séquence en 5′ parmi celles des 32 séquences testées qui ne présentent pas d’hétérogénéité en 5ʹ′. La seconde solution est d’utiliser une étiquette clivable en 5ʹ′ de l’ARN d’intérêt, tel que le ribozyme hammerhead, déjà utilisée pour ce genre d’application, ou le système CRISPR/Cse3 que nous proposons dans l’article présenté dans ce mémoire. De plus, nous avons adapté la méthode ARiBo pour rendre possible la purification d’un long ARN de 614 nt, le polycistron miR-106b-25. Nous avons également démontré la possibilité d’utiliser la méthode ARiBo pour l’isolation de protéines qui se lient à un ARN donné, le précurseur de miRNA pre-miR-153-2. En conclusion, ce mémoire démontre la possibilité d’adapter la méthode ARiBo à plusieurs applications. / In recent years, the field of RNA research has exploded due to several discoveries demonstrating the importance of RNA in many biological processes. Along with the increased interest in this field, large amounts of RNA have become essential to the success of several studies, in particular for structural biology and functional characterization. However, there are still very few native purification methods that are simple, efficient and reliable. In the past few years, the Legault laboratory has established an affinity purification method using an ARiBo tag to purify RNAs produced by in vitro transcription with the T7 RNA polymerase. This RNA purification method was specifically developed to maximise purity and yield. In addition, this method is fast and works with several types of RNAs. However, like several other purification methods, this method produces RNAs with 5' heterogeneity. This Master’s thesis propose solutions to overcome the problem of 5' heterogeneity for RNAs transcribed with the T7 RNA polymerase and purified with the ARiBo method. The first solution proposed is to choose a 5' sequence among those of the 32 sequences tested that do not present 5'- heterogeneity. The other possibility is the use of a cleavable tag at the 5'-end of the RNA of interest, such as the hammerhead ribozyme, already used for this purpose or the CRISPR/Cse3 system, which is presented here. Furthermore, we have adapted the ARiBo method to purify an RNA of 614 nt, the miRNAs cluster miR- 106b-25. We also demonstrate the possibility to use the ARiBo method to isolate proteins that bind a given RNA, the miRNA precursor pre-miR-153-2. In conclusion, this Master’s thesis demonstrates the possibility of adapting the ARiBo method for several applications.

Uppsatsens syfte är att kartlägga hur nio svenska turistföretag ser på sociala mediers och konsumentens roll inom marknadsföring online. Vidare vill uppsatsförfattarna analysera potentiella effekter av virala marknadsföringskampanjer. Detta utifrån fyra teoretiska ramverk: webb 2.0, word-of-mouth, viral marknadsföring samt social valuta. I uppsatsens analys besvaras tre frågeställningar: hur ser turistföretagen på marknadsföring genom sociala medier, vad har konsumenternas sociala nätverk för betydelse i turistföretagens virala marknadsföring, samt vilka är de upplevda effekterna av virala marknadsföringskampanjer? Uppsatsens empiri baseras på data insamlad via nio semistrukturerade intervjuer. De generella resultaten visar på att konsumentens sociala nätverk online har en stor betydelse för företagens marknadsföring online, konsumenterna äger varumärket genom att de i sina sociala kanaler kan sprida både positiv och negativ word-of-mouth till det sociala nätverket. Sociala medier är en viktig faktor i turistföretagens marknadsföring och företagen måste idag vara närvarande på sociala medier då allt fler konsumenter befinner sig online. Studien har även visat att företagen endast upplever positiva effekter av företagens virala marknadsföringskampanjer. Kampanjerna leder till att konsumenterna integreras i marknadsföringen och sprider företagets varumärke till dess sociala nätverk. I och med detta bildas en positiv relation mellan företag och konsument. / The aim of this essay is to identify how nine Swedish tourism companies view the role of social media and the consumer in online marketing. Furthermore, the authors will analyze the potential effects of viral marketing campaigns. This is based on four theoretical frameworks: web 2.0, word-of-mouth, viral marketing and social currency. The analysis answer three questions: how tourism companies looks on marketing through social media, what consumer social networks importance of tourism businesses viral marketing, and what are the perceived effects of viral marketing campaigns? The essays empirics are based on data collected through nine semi-structured interviews. The overall results show that the social networks of consumers have a great significance for the companies’ online marketing. Through their social media channels, consumers can spread both positive and negative word-of-mouth to their social networks, this has the effect that consumers now can be said to own the companies’ brands. Social media is an important element of the tourism companies’ marketing strategies. Consumers increasing usage of social media means that companies today must be present online. Furthermore, the study has shown that tourism companies have only seen positive effects of their viral marketing campaigns. The campaigns have the effect of consumers integrating with the companies’ marketing and spreading the companies’ brands within their social networks. This creates a positive relation between company and consumer.

Made available in DSpace on 2016-04-25T16:44:55Z (GMT). No. of bitstreams: 1 Carlos Vital Giordano.pdf: 1165460 bytes, checksum: cfb404d6f557d208e96fb9400eb40248 (MD5) Previous issue date: 2007-05-16 / The present research aims to study the benefits that could be possible by using the technology supported by radio frequency for identification purposes. The RFID (radio frequency identification) allows the manufacturing the identification tags (smart labels) whose, accordingly fixed in products, boxes, and even in pallets, induct starting sensible modifications in organizations management supply chain, since supported by integrated information systems, both internal and external. The method used was the case study, supported by the creation of some question matrixes used for semi-structured interviews and questions applied on professionals working on development and implementation of fundamental solutions in this core technology, on the environment of relationships involving supply among companies. Initially were identified the contributions brought by companies where interviewed subjects works, for solution of the context studied. Following, the systemic and optimized operational stages were determined for the implementation of those considered more satisfactory ones, considering the quantitative analysis results. The conclusion demonstrates the existence of strong evidences concerning investment returns and operational success, as well as strong evidences on improvement of strategic level, further good evidences of improvements on management level. The results emphasized that, besides the slow evolution at moment, the recommended insertion of technology is mandatory and irreversible, when dissemination will occur. Further, strong similarity was encountered related to development and dissemination of bar code technology, on the supply chain context / Esta pesquisa tem por objetivo estudar as melhorias possibilitadas por uma tecnologia que utiliza como base de funcionamento a rádio freqüência, com propósitos de identificação. A RFID (radio frequency identification, identificação por rádio freqüência), permite a manufatura de tags (etiquetas), que devidamente colocadas em itens, caixas ou paletes servem de componente iniciador de sensíveis alterações na gestão da cadeia de suprimentos de produtos das organizações, desde que corretamente suportadas pelos sistemas de informações integrados, tanto internos como externos à empresa. O método de pesquisa utilizado foi o estudo de caso, tendo como suporte a criação de matrizes de questionamentos utilizadas para entrevistas semiestruturadas e questionários aplicados a profissionais que trabalham em desenvolvimento e implementação de soluções fundamentadas na tecnologia foco, no ambiente das relações envolvendo suprimentos entre empresas. Foram identificadas inicialmente as colaborações da empresa dos entrevistados nas soluções para o contexto estudado. A seguir foram determinadas as etapas sistêmicas e operacionais otimizadas para as implementações das soluções entendidas como as mais satisfatórias, considerando a efetivação da análise em termos qualitativos. A conclusão mostra que há fortes evidências de retornos e de sucesso no nível operacional, que há fortes evidências de melhorias no nível estratégico e que há boas evidências de melhoramentos no nível gerencial. Realçam os resultados, a preconização de que a inclusão da tecnologia no ambiente estudado, apesar de no momento se encontrar em compasso lento, é mandatória, iminente e de características irreversíveis quando acontecer sua disseminação, encontrando ainda, semelhanças acentuadas aos passos dados durante o aparecimento e a consolidação do código de barras no contexto da cadeia de suprimentos

The biofuel market is dominated by ethanol and biodiesel derived from cellulosic and lipid-based biomass crops. This is largely due to the relatively low costs and reliability of production. At present, production of non-food plant-derived oils for biofuel production in the U.S. is minimal. A research team from the Commonwealth Scientific and Industrial Research Organization (CSIRO), an independent Australian federal government research institution, has developed an efficient transgenic system to engineer oil production in tobacco leaves. This novel system is comprised of multiple transgenes that direct the endogenous metabolic flux of oil precursors towards triacylglycerol (TAG) production. Additional genes were incorporated to store and protect the accumulated oil in vegetative tissues. Preliminary greenhouse tests by the CSIRO research group indicated an oil content of > 30% by dry weight (DW) in tobacco leaf lamina. Here we evaluated two transgenic lines against a non-transgenic control in 2017 and 2018 in greenhouse and field production systems. The 2017 pilot study showed that the high leaf-oil tobacco line was viable and will grow in the field in Kentucky. Chemical analyses revealed significantly higher oil content compared to the non-transgenic control despite several logistical setbacks. These promising discoveries prompted the deployment of additional transgenic line assessments and further data validation in 2018. Line evaluations in 2018 revealed that the LEC2:WRI1:DGAT:OLE transgenic line had the highest leaf oil content (≥ 19.3% DW-1) compared to both the WRI1:DGAT:OLE transgenic line (≤ 5.6% DW-1) and non-transgenic control (≤ 2.1% DW-1). The results of this research will contribute to the successful development of transgenic tobacco lines engineered to accumulate high concentrations of TAG in the leaves.

隨著時間的推移演進，人們瞭解到將知識經驗著作成文獻典籍保存下來供後人研究開發的重要性。時至今日，以英語為主的學術寫作論文成為全世界最主要的研究交流媒介。而對於英語為非母語的研究專家而言，在進行英語學術寫作上常常會遇到用了不適當的字彙或搭配詞導致無法確切的傳達自己的研究成果，或是在表達上過於貧乏的問題，因此英語學術寫作字彙與搭配詞的學習與使用就顯得相當重要。 在本研究中，我們藉由收集大量不同國家以及不同研究領域的學術論文為基礎，建構現實中實際使用的語料庫，並且建立數種詞性標籤型態，使用關鍵詞擷取關鍵詞擷取(Keyword Extraction)技術從中擷取出學術著作中常用的學術寫作字彙候選詞，當作是學術常用寫作字彙之初步結果，隨即將候選詞導入關鍵詞分析的指標形態模型，將候選詞依照指標特徵選出具有代表指標意義的進一步候選詞。 在實驗方面，透過對不同範圍的樣本資料進行篩選，並導入統計上的方法對字彙進行不同領域共通性的分析檢證，再加上輔助篩選的機制後，最後求得名詞和動詞分別在學術寫作中常用的字彙，也以此字彙為基礎，發掘出語料庫中常用的搭配詞組合，提出以英語為外國語的研究學者以及學生在學術寫作上的常用字彙與搭配詞組合作為參考，在學術寫作上能夠提供更多樣性且正確的研究論述的協助。 / With the evolution over time, people start to know the importance of taking their knowledge and experience into literature texts and preserving them for future research. Until now, academic writing research papers mainly in English become the world’s leading communication media all over the world. For those non-native English researchers, they often encounter with the inappropriate vocabularies or collocations which causes them not to pass on their idea accurately or to express their research poorly. As a result, it’s very important to know how to learn or to use the correct academic writing in English vocabularies and collocations. In this study, we constructed the real academic thesis corpus which includes different countries and fields of academic research. The keyword extraction technique based on the several Part-of-Speech tag patterns is used for capturing the common academic writing vocabulary candidates in the academic works to be the initial result of the common vocabulary of academic writing. The candidate words would be introduced to the index analysis model of keyword and be picked out to the further meaningful candidate words according to the index characteristics. For the experiments, the sample data with different fields would be filtered and the vocabularies on different fields of commonality would be analyzed and verified through statistical methods. Moreover, the auxiliary filter mechanism would also be applied to get the common vocabularies in academic writing with nouns and verbs. Based on these vocabularies, we could discover the common combination with the words in the academic thesis corpus and provide them to the non-native English researchers and students as a reference with the common vocabularies and collocations in academic writing. Hopefully the study could help them to write more rich and correct research papers in the future.